CN110951824A - Application of composite buffer system in preparation of α -amylase determination kit - Google Patents
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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Abstract
The invention discloses an application of a composite buffer system in preparing α -amylase determination kits, wherein the composite buffer system is a mixed buffer solution containing MES, ACES and PIPES, the α -amylase determination kit has good stability, and simultaneously ensures the accuracy of the detection result of the kit, the α -amylase determination kit containing the composite buffer system of the scheme of the invention can be stably stored at (2-8) DEG C for more than 21 months, and the kit has low cost and simple preparation, and is worthy of wide popularization.
Description
Technical Field
The invention relates to the technical field of in-vitro diagnosis, in particular to application of a composite buffer system in preparation of α -amylase determination kits.
Background
Serum Amylase, namely blood Amylase, is one of Amylase, is mainly secreted by salivary glands and pancreas, and is also secreted in a small amount in organs such as proximal duodenum, lung, uterus, mammary gland in lactation period, the function of the serum Amylase is mainly to decompose polysaccharides, such as starch and glycogen, the serum Amylase assay is the most commonly used diagnostic index for acute pancreatitis, more than 200 methods for serum Amylase assay in various clinical laboratories in China are provided at present, the reaction principle is different, and the standardization of the serum Amylase assay is difficult, the most effective means for unifying the assay results is to establish a reference system, the international clinical chemistry and inspection medicine combination (international patent medicine and laboratory medicine, IFCC) shows the influence of reagent raw materials on the measurement results in a first-level reference measurement program (37 ℃) published in 2006, 5639-Amylase (α -Amylase, AMY) is a commonly used Amylase assay raw material, the characteristic α -glucosidase and 4-Ethylidene-4-phenylethyl-4834-NPC specified in the reference method are used as Amylase assay raw materials, the stability of starch-Amylase, the Amylase-6-NPK-3635-NPK-5, the stability of the serum Amylase-NPK-5, the protein is used as a stable reagent kit for detecting the protein, the stability of the protein, the protein-beta-Amylase, the protein.
The buffer solution is usually a pair of conjugates composed of weak acid and its weak acid salt or weak base and its weak base salt, and is mainly used to maintain the pH value and ionic strength of the system in biochemical research work, so as to ensure that the reaction system is in dynamic balance. Different buffer systems are required to be selected for different reaction systems, and the common buffer solution achieves the buffer effect by utilizing single weak acid-conjugate base or acid-base balance between weak base-conjugate acid. However, for some reaction systems, a buffer system consisting of a single acid and base is difficult to meet. Pridraux proposed in 1916 a broad buffer system with substantially unchanged coexisting ions but with a broadened pH range (between 2 and 12), which was modified to be designated as Britton-Robinson broad buffer. Besides maintaining the pH value of the system, the ionic strength of the buffer solution also has a great effect on the stability of the biochemical reaction reagent, so that the control of the pH value and the ionic strength has an important significance on the maintenance of the stability of the biochemical reaction reagent.
Based on the above, the development of a composite buffer system is expected to solve the defect of poor stability of the α -amylase determination kit in the prior art.
Disclosure of Invention
Therefore, the invention provides the application of a composite buffer system in the preparation of α -amylase determination kit, and the composite buffer system can improve the stability of α -amylase determination kit.
The invention also provides an α -amylase determination kit containing the composite buffer system.
The invention also provides a method for detecting α -amylase by using the kit.
Use of a complex buffer system according to an embodiment of the first aspect of the invention, which is a mixed buffer containing 2- (N-morpholine) ethanesulfonic acid (2-Morpholinoethanesulfonic acid, MES), N- (Acetamido) -2-Aminoethanesulfonic acid (N- (2-Acetamido) -2-Aminoethanesulfonic acid, ACES) and Piperazine-1, 4-disulfonic acid (piperine-1, 4-bisethanesulfonic acid, PIPES) in the preparation of α -amylase assay kit.
According to some embodiments of the invention, in the composite buffer system, the MES content is (1-2)%, the ACES content is (0.5-1)%, and the PIPES content is (0.5-1)%.
According to some embodiments of the invention, in the composite buffer system, the MES is 1% by mass, the ACES is 0.8% by mass, and the PIPES is 0.5% by mass.
According to some embodiments of the present invention, the α -amylase assay kit further comprises a first reagent and a second reagent, wherein the first reagent is a complex buffer system dissolved with α -gluconidase, sodium chloride, tween-20 and sodium azide, and the second reagent is a complex buffer system dissolved with pNP-G7 and sodium azide.
According to some embodiments of the invention, the first reagent comprises α -glucopsidase 5KU/L, sodium chloride 0.1G/L, tween-200.1G/L and sodium azide 0.1G/L, and the second reagent comprises pNP-G710G/L and sodium azide 0.1G/L.
According to some embodiments of the invention, the volume ratio of the first reagent to the first reagent is 3: 1.
According to some embodiments of the invention, the applying comprises the steps of:
the first reagent is prepared by weighing MES, ACES and PIPES, dissolving in water, adding Tween-20 as emulsifier, emulsifying, adding biological antiseptic such as sodium azide, ionic stabilizer such as sodium chloride, α -glucopyranosidase and water, stirring, and filtering;
preparation of the second reagent: weighing MES, ACES and PIPES, adding water to dissolve, adding biological antiseptic such as sodium azide, pNP-G7 and water, stirring, and filtering.
According to some embodiments of the invention, the kit further comprises a calibrator in the raw materials for preparation.
The application of the embodiment of the invention has at least the following beneficial effects that a reaction system of the amylase determination kit in the scheme of the invention adopts various buffer solutions to control pH value and ionic strength so as to ensure the reaction strength and stability of the reagent, the principle of the α -amylase determination kit is that amylase cleavage substrate 4, 6-ethylene-p-nitrophenyl- α -D-maltoheptaoside (Ethylidene-4-nitrophenyl-a-D-maltoheptaside, EPS-pNP-G7), the products of G2 pNP, G3 pNP and G4 pNP can be hydrolyzed into p-nitrophenol and glucose by glucosidase, the generation amount of p-nitrophenol is in proportion to AMY activity in a certain range, the activity of AMY can be continuously monitored at 405nm/700nm, the preservation time of the glucosidase in the reagent R1 can be directly influenced to clinical use, therefore, the mixed buffer solution is adopted in the scheme of the invention to establish a stable ion preservation system for the glucosidase, the preservative can be used as a certain amount of preservative, the preservative can be added into micro-air, the preservative can be added, the preservative can be used for promoting micro-pollution of the biological air, and the preservative can be added, and the biological preservative can be used for prolonging the biological use, the biological preservative can be used for promoting the growth of the detection of the biological detection of7The light absorption coefficient is easily influenced by pH, temperature, protein concentration in a sample, ionic strength, an activator and the like, and the mixed buffer system added with the scheme of the invention can be PNP-G7Isolate itA series of interferences ensure that PNP-G7And further the stability of the whole kit is improved.
According to the kit of the second aspect embodiment of the present invention, the kit contains a complex buffer system, and the complex buffer system is a buffer solution containing MES, ACES and PIPES.
The kit provided by the embodiment of the invention has at least the following beneficial effects that the α -amylase determination kit containing the composite buffer system provided by the invention can be stably stored for more than 21 months at the temperature of (2-8). The kit is low in cost, simple to prepare and worthy of further wide popularization.
The detection method comprises the following steps of adding the detection reagent in the kit into a sample to be detected for catalytic reaction, detecting a reaction product by using a detector after the reaction is finished, recording a detection result, and calculating α -amylase concentration according to the detection result.
The detection method according to the embodiment of the third aspect of the invention has at least the following beneficial effects that the α -amylase determination kit has good stability, and meanwhile, the accuracy of the detection result of the kit is ensured.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Drawings
FIG. 1 is a comparison of the sera of example 200 of the kit for determination of amylase α and Roche α -amylase diagnostic kit in the examples of the present invention.
Detailed Description
In order to explain technical contents, achieved objects, and effects of the present invention in detail, the following description is made with reference to the accompanying drawings in combination with the embodiments.
The following examples of the present invention relate to apparatus comprising: hitachi full-automatic biochemical analyzer 1 (Hitachi 7020, instruments with similar functions of other brands can be used).
The embodiment of the invention is an application of a composite buffer system in preparation of a α -amylase determination kit, wherein the preparation raw materials of the determination kit comprise a reagent 1 and a reagent 2, the reagent 1 is the composite buffer system dissolved with α -glucondase 5KU/L, sodium chloride 0.1G/L, tween-200.1G/L and sodium azide 0.1G/L, the reagent 2 is the composite buffer system dissolved with pNP-G710G/L and sodium azide 0.1G/L, the composite buffer system contains 1 wt% MES, 0.8 wt% ACES and 0.5 wt% PIPES, and after the effective components (MES, ACES and PIPES) in the composite buffer system are fully dissolved and mixed, other components are added, so that the reagent R1 and the reagent R2 of the kit can be formed.
And evaluating the three aspects of the long-term stability validity period, the accuracy and the proportion of the composite buffer system of the kit prepared by the operation.
(1) Long term stability expiration date and accuracy
The test takes British Lange serum calibrator 3(CAL2351-914UE), quality control level 2(HN1530-1044UN) and serum quality control level 3(HE1532-793UE) as samples, the standard is British Lange conventional calibrator, a Hitachi 7020 full-automatic biochemical analyzer is adopted for detection, a blank test (the sample is replaced by physiological saline) and two sample tests are set, the ratio of the sample to the reagent 1 and the reagent 2 is 8:180:60 muL, the main wavelength is set to be 405nm and the auxiliary wavelength is set to be 700nm, the sample and the reagent 1 are uniformly mixed, incubation is carried out for 5min at 37 ℃, the absorbance A1 of each tube is read, the reagent 2 is added for reaction for 5min after uniform mixing, the absorbance A2 is read, the absorbance change delta A of each tube is A2-A1, the absorbance change delta A of the sample and the standard is calculated after the blank of the reagent is deducted, and then the content of α -amylase in the sample is automatically calculated, the target value of the starch control enzyme in quality control 2 is α -5393U/α U, and the allowable deviation of the starch in 67233L/233L range.
According to the determination method, the quality control 2 and the quality control 3 in the same batch and the same bottle are used as detection samples, the α -amylase determination kit detects once every period of time, the detection period is 20 months, and the stability result is judged from three aspects:
first, the calculated relative Deviation, Standard Deviation (SD), and Coefficient of Variation (CV) of the measurement control results are shown in table 1, and the stability of the measurement results can be reflected by the relative Deviation and Coefficient of Variation (CV):
TABLE 1 Change in quality control over time
As can be seen from Table 1, the quality control of the α -amylase assay kit of the example begins to decrease after 21 months, and the precision CV is less than 5%.
Second, calculate and determine another batch number of calibrator (CAL2351-877UE), national general chemical laboratory quality assessment sample and health department accuracy verification sample relative deviation to reflect the accuracy of the kit, the results are shown in Table 2, Table 3 and Table 4.
TABLE 2 determination of CAL2351-877UE behavior over time
| Time (moon) | 1 | 5 | 8 | 10 | 12 | 14 | 18 | 21 | 23 |
| Indicating value | 228 | 228 | 228 | 228 | 228 | 228 | 228 | 228 | 228 |
| Test 1 | 227 | 218.5 | 232.3 | 206.8 | 231 | 212.2 | 229 | 210.1 | 194 |
| Test 2 | 231 | 217.3 | 232 | 212.1 | 235.6 | 217.2 | 226 | 209.2 | 192.2 |
| Test 3 | 227 | 218.5 | 232 | 220.3 | 231.6 | 216 | 227 | 208.5 | 192 |
| Mean value of | 228.3 | 218.1 | 232.1 | 213.1 | 232.7 | 215.1 | 227.3 | 209.3 | 192.7 |
| Deviation of | 0.13％ | -4.34％ | 1.80％ | -6.54％ | 2.06％ | -5.66％ | -0.31％ | -8.22％ | -15.48％ |
As can be seen from Table 2, the variation of the calibrator for the next lot measured at month 21 had begun to increase, and by 23 months the variation had exceeded 10%. The test kit can keep good stability within 21 months.
TABLE 32019 national quality assessment of conventional chemistry in Chamber
| Sample numbering | Measurement results | Target value | Bias (%) | Allowable range | Evaluation results |
| 201911 | 225 | 214 | 5.14 | 182-246 | By passing |
| 201912 | 72 | 71 | 1.41 | 60-82 | By passing |
| 201913 | 474 | 445 | 6.52 | 378-512 | By passing |
| 201914 | 249 | 239 | 4.18 | 203-275 | By passing |
| 201915 | 344 | 325 | 5.85 | 276-374 | By passing |
TABLE 42019 statistical results of the quality assessment of amylases in laboratory on the accuracy of enzymology
| Specimen (variants) | Mean value of | Target value | Bias (%) | Evaluation Range | 2 standard deviation | Coefficient of variation (%) | Evaluation ofResults |
| 201901 | 66.4 | 66.00 | 0.61 | 61.05-70.95 | 1.80 | 1.40 | By passing |
| 201902 | 287.8 | 301.95 | -4.69 | 279.30-324.60 | 6.00 | 1.00 | Not evaluated |
As can be seen from tables 3 and 4, the national conventional chemical laboratory evaluation and the accuracy verification of the kit are passed, which indicates that the kit has good accuracy of the measurement result.
Thirdly, 200 sera were measured simultaneously with the Roche α -amylase assay kit (EPS method) in a hospital, and the results are shown in FIG. 1 (y in FIG. 1 is the test result of the kit according to the embodiment of the present invention, and x is the test result of the Roche α -amylase diagnostic kit) and Table 5:
TABLE 5 results of the determination of 200 sera with the α -Amylase assay kit and the Roche α -Amylase diagnostic kit of this example
As can be seen from table 5 and fig. 1, 200 sera were measured using the well-performing roche α -amylase assay kit (EPS method) as the comparison kit, wherein the abnormal sample accounts for 30% of the ratio, and the linear regression equation y (0.9827 x-1.898) of the two kits shows that the a value (0.9827) is close to 1 and the b value (-1.898) is close to 0, indicating that the reagent fitting degree in the two kits is good.
In conclusion, the composite buffer system and other components in the scheme of the invention provide good stability, accuracy and precision for the α -amylase determination kit.
Proportioning experiment of (II) composite buffer system
1. A composite buffer system: preparing composite buffer systems with different proportions, comprising:
proportioning 1: 1% MES buffer, 1% ACES buffer, 1% PIPES buffer;
and (2) proportioning: 1% MES buffer, 0.5% ACES buffer, 1% PIPES buffer;
proportioning 3: 1% MES buffer, 1% ACES buffer, 0.5% PIPES buffer;
and (4) proportioning: 0.5% MES buffer, 1% ACES buffer, 1% PIPES buffer;
and (2) proportioning 5: 1% MES buffer, 0.8% ACES buffer, 0.5% PIPES buffer.
2. The α -amylase assay kit for the test consists of a reagent R1 and a reagent R2:
α -glucopsidase 5KU/L, sodium chloride 0.1g/L, Tween-200.1 g/L, and sodium azide 0.1g/L as reagent 1.
Reagent 2, pNP-G710G/L, sodium azide 0.1G/L.
3 test method and results:
the 5 compound buffer systems in the mixture ratio are respectively added into an α -amylase determination kit (a reagent R1 and a reagent R2 are added according to the same amount), the determination of the content of α -amylase in a sample is carried out, the relative deviation (CV), the Standard Deviation (SD) and the relative standard deviation (CV) of the determination quality control and CAL2351-877UE results are calculated according to the determination method of the stability test of the invention, and the results are shown in the following table 6:
TABLE 6 data statistics table for composite buffer system determination quality control and CAL2351-877UE with different proportions
As can be seen from the data in the table, the results of the quality control products with high and low concentration levels and CAL2351-877UE measured by five composite buffer systems with different proportions are compared, and the proportion 5 is determined to be the optimal proportion.
The analysis results of all the data show that the α -amylase assay kit with the proportion can maintain the storage time of 21 months under the action of a composite buffer system and other stabilizers, the results of the laboratory evaluation and the accuracy verification are qualified, the test stability, the accuracy and the precision are effectively improved, and the α -amylase assay kit has the advantages of low composite stabilizer cost, simple preparation and worth further popularization and use.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all equivalent changes made by using the contents of the present specification and the drawings, or applied directly or indirectly to the related technical fields, are included in the scope of the present invention.
Claims (10)
1. The application of the composite buffer system in the preparation of α -amylase determination kit is characterized in that the composite buffer system is a mixed buffer containing MES, ACES and PIPES.
2. Use according to claim 1, characterized in that: in the composite buffer system, the mass percentage of MES is (1-2)%, the mass percentage of ACES is (0.5-1)%, and the mass percentage of PIPES is (0.5-1)%.
3. Use according to claim 2, characterized in that: in the composite buffer system, the mass percentage of MES is 1%, the mass percentage of ACES is 0.8%, and the mass percentage of PIPES is 0.5%.
4. The use according to any one of claims 1 to 3, wherein the α -amylase assay kit further comprises a first reagent and a second reagent in the raw materials for preparing, wherein the first reagent is a complex buffer system dissolved with α -glucopsidase, sodium chloride, Tween-20 and sodium azide, and the second reagent is a complex buffer system dissolved with pNP-G7 and sodium azide.
5. The use of claim 4, wherein the first reagent comprises α -glucopsidase 5KU/L, sodium chloride 0.1G/L, Tween-200.1G/L and sodium azide 0.1G/L, and the second reagent comprises pNP-G710G/L and sodium azide 0.1G/L.
6. Use according to claim 5, characterized in that: the volume ratio of the first reagent to the first reagent is 3: 1.
7. Use according to claim 4, characterized in that: the application comprises the following steps:
the first reagent is prepared by weighing MES, ACES and PIPES, dissolving in water, adding Tween-20 as emulsifier, emulsifying, adding biological antiseptic such as sodium azide, ionic stabilizer such as sodium chloride, α -glucopyranosidase and water, stirring, and filtering;
preparation of the second reagent: weighing MES, ACES and PIPES, adding water to dissolve, adding biological antiseptic such as sodium azide, pNP-G7 and water, stirring, and filtering.
8. Use according to any one of claims 1 to 3, characterized in that: the preparation raw materials of the kit also comprise a calibrator.
9. An α -amylase assay kit is characterized in that the kit contains a composite buffer system, and the composite buffer system is a buffer solution containing MES, ACES and PIPES.
10. A detection method of α -amylase is characterized by comprising the following steps of adding a detection reagent in the kit according to claim 9 into a sample to be detected for catalytic reaction, detecting a reaction product by using a detector after the reaction is finished, recording a detection result, and calculating the concentration of α -amylase according to the detection result.
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4945043A (en) * | 1986-06-25 | 1990-07-31 | Boehringer Mannheim Gmbh | Process and reagent for the determination of α-amylase |
| ATE97131T1 (en) * | 1986-07-11 | 1993-11-15 | Wako Pure Chem Ind Ltd | METHOD FOR DETERMINING ALPHA-AMYLASE USING MODIFIED OLIGOSACCHARIDS AND METHOD FOR THEIR PREPARATION. |
| JP2000189194A (en) * | 1998-12-30 | 2000-07-11 | Shino Test:Kk | Reagent and method for measuring alfa-amylase activity |
| JP2004325280A (en) * | 2003-04-25 | 2004-11-18 | Toyobo Co Ltd | Stabilization method for detecting substrate and reagent therefor |
| CN103207156A (en) * | 2013-03-28 | 2013-07-17 | 山东博科生物产业有限公司 | Stable alpha-amylase reagent and its application |
| CN104198691A (en) * | 2014-08-14 | 2014-12-10 | 上海睿康生物科技有限公司 | Stable alpha-amylase detection kit |
| CN105021543A (en) * | 2015-06-30 | 2015-11-04 | 广州金域医学检验中心有限公司 | Alpha-amylase detection reagent and application thereof |
| JP2016123302A (en) * | 2014-12-26 | 2016-07-11 | キッコーマン株式会社 | Amadoriase stable to good-buffer |
| JP2018011556A (en) * | 2016-07-21 | 2018-01-25 | 関東化學株式会社 | Amylase activity measurement reagent and amylase activity measurement method |
| US20190018025A1 (en) * | 2015-12-31 | 2019-01-17 | Siemens Healthcare Diagnostics Inc. | Non-recombinant human insulin-like growth factor binding protein concentrate |
-
2019
- 2019-11-15 CN CN201911116972.6A patent/CN110951824B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4945043A (en) * | 1986-06-25 | 1990-07-31 | Boehringer Mannheim Gmbh | Process and reagent for the determination of α-amylase |
| ATE97131T1 (en) * | 1986-07-11 | 1993-11-15 | Wako Pure Chem Ind Ltd | METHOD FOR DETERMINING ALPHA-AMYLASE USING MODIFIED OLIGOSACCHARIDS AND METHOD FOR THEIR PREPARATION. |
| JP2000189194A (en) * | 1998-12-30 | 2000-07-11 | Shino Test:Kk | Reagent and method for measuring alfa-amylase activity |
| JP2004325280A (en) * | 2003-04-25 | 2004-11-18 | Toyobo Co Ltd | Stabilization method for detecting substrate and reagent therefor |
| CN103207156A (en) * | 2013-03-28 | 2013-07-17 | 山东博科生物产业有限公司 | Stable alpha-amylase reagent and its application |
| CN104198691A (en) * | 2014-08-14 | 2014-12-10 | 上海睿康生物科技有限公司 | Stable alpha-amylase detection kit |
| JP2016123302A (en) * | 2014-12-26 | 2016-07-11 | キッコーマン株式会社 | Amadoriase stable to good-buffer |
| CN105021543A (en) * | 2015-06-30 | 2015-11-04 | 广州金域医学检验中心有限公司 | Alpha-amylase detection reagent and application thereof |
| US20190018025A1 (en) * | 2015-12-31 | 2019-01-17 | Siemens Healthcare Diagnostics Inc. | Non-recombinant human insulin-like growth factor binding protein concentrate |
| JP2018011556A (en) * | 2016-07-21 | 2018-01-25 | 関東化學株式会社 | Amylase activity measurement reagent and amylase activity measurement method |
Non-Patent Citations (6)
| Title |
|---|
| KLAUS LORENTZ 等: "Evaluation of a Direct α-Amylase Assay Using 2-Chloro-4-nitrophenyl-α-D-maltotrioside", 《CLIN CHEM LAB MED》 * |
| KLAUS LORENTZ 等: "Evaluation of a Direct α-Amylase Assay Using 2-Chloro-4-nitrophenyl-α-D-maltotrioside", 《CLIN CHEM LAB MED》, vol. 37, no. 11, 31 December 1999 (1999-12-31) * |
| 孙慧颖等: "试剂原料对α-淀粉酶参考方法测定结果影响的临床研究", 《检验医学与临床》 * |
| 孙慧颖等: "试剂原料对α-淀粉酶参考方法测定结果影响的临床研究", 《检验医学与临床》, no. 03, 14 February 2016 (2016-02-14) * |
| 宋焰桃: "两种试剂测定血清α-淀粉酶的方法比对和偏倚评估", 《国际检验医学杂志》 * |
| 宋焰桃: "两种试剂测定血清α-淀粉酶的方法比对和偏倚评估", 《国际检验医学杂志》, vol. 33, no. 7, 30 July 2012 (2012-07-30) * |
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