CN110947440B

CN110947440B - Dual-temperature-zone device for centrifugation and microfluidic analysis device

Info

Publication number: CN110947440B
Application number: CN201911105029.5A
Authority: CN
Inventors: 陈运
Original assignee: Shenzhen Gangzhu Medical Technology Co ltd
Current assignee: Shenzhen Chenghui Medical Technology Co.,Ltd.
Priority date: 2019-11-08
Filing date: 2019-11-08
Publication date: 2021-11-02
Anticipated expiration: 2039-11-08
Also published as: CN110947440A

Abstract

The application relates to a dual-temperature-zone device and a microfluidic analysis device for centrifugation, wherein the dual-temperature-zone device is provided with an inner temperature zone and an inner temperature zone opening, and an inner temperature zone heating assembly is arranged in the inner temperature zone of the dual-temperature-zone device; the dual-temperature-zone device is provided with an outer temperature zone and an outer temperature-zone opening, and an outer temperature-zone heating assembly is arranged in the outer temperature zone of the dual-temperature-zone device; the heat-insulating isolation structure is arranged between the inner temperature zone and the outer temperature zone, the heat-insulating isolation structure is provided with a groove so that one part of the structure to be centrifuged is accommodated in the inner temperature zone and the other part of the structure to be centrifuged is accommodated in the outer temperature zone, and the outer temperature zone is provided with an outer temperature zone inlet and a sealing cover structure. The centrifugal temperature control system is suitable for a centrifugal system with differentiated temperature requirements, on one hand, different requirements of different reaction stages on temperature can be met, on the other hand, the centrifugal temperature control system is suitable for a more complex reaction system, different temperature reaction systems aiming at different temperature rise rates can be designed, and on the other hand, the design of an outer temperature zone inlet is beneficial to realizing air cooling so as to improve the temperature drop rate of the outer temperature zone.

Description

Dual-temperature-zone device for centrifugation and microfluidic analysis device

Technical Field

The application relates to the field of centrifugal microfluidics, in particular to a dual-temperature-zone device for centrifugation and a microfluidic analysis device.

Background

A fully automatic nucleic acid analyzer is an apparatus for performing nucleic acid analysis by PCR (polymerase chain reaction). The device can realize the full automation of sample cracking, nucleic acid purification and PCR reaction of molecular diagnosis by matching with a centrifugal microfluidic chip. The technology belongs to the technical field of molecular diagnosis and also belongs to the technical field of centrifugal microfluidics.

The molecular diagnosis based on PCR amplification is to detect the existence of endogenous (genetic or variant) or exogenous (pathogen) target gene by primer-mediated specific amplification of the target gene, and further provide information and decision basis for disease diagnosis and treatment. Microfluidic (Microfluidics) systems refer to devices that manipulate liquids on a sub-millimeter scale, typically from a few microns to hundreds of microns. It integrates the basic operation units related to the biological and chemical fields, even the functions of the whole laboratory, including sampling, diluting, reacting, separating, detecting, etc. on a small Chip, so it is also called Lab-on-a-Chip. The chip generally comprises various liquid storage tanks and a micro-channel network which is connected with each other, can greatly shorten the sample processing time, and realizes the maximum utilization efficiency of reagent consumables by precisely controlling the liquid flow. Centrifugal microfluidics belongs to a branch of microfluidics, and particularly relates to a device for controlling liquid on a sub-millimeter scale by using centrifugal force through rotating a centrifugal microfluidic chip. It integrates the basic operation units involved in the fields of biology and chemistry on a small disc-shaped (disc-shaped) chip. In addition to the advantages specific to microfluidics, the overall device is more compact since only one motor is required for centrifugal microfluidics to provide the force required for liquid manipulation. And the ubiquitous centrifugal field on the disc chip can not only make liquid drive more effective and ensure that no liquid remains in the pipeline, but also can effectively realize sample separation based on density difference and make parallel processing simpler. Therefore, centrifugal microfluidics is also increasingly used in Point-of-care testing (POCT).

The molecular diagnosis based on PCR amplification is to detect the existence of endogenous (genetic or variant) or exogenous (pathogen) target gene by primer-mediated specific amplification of the target gene, and further provide information and decision basis for disease diagnosis and treatment. The main application scenes of the kit include diagnosis of infectious diseases, blood screening, tumor mutation site detection, diagnosis of genetic diseases, prenatal diagnosis, tissue typing and the like. Molecular diagnostics based on PCR amplification generally comprise the following steps: sample cracking, nucleic acid purification, nucleic acid amplification under the constraint of specific primers, and fluorescent signal acquisition and analysis. Although the advantages of molecular diagnosis technology are obvious, the steps are complicated, the process is time-consuming, professional operation is needed, and the construction cost of a clinical molecular diagnosis laboratory is generally high, so that the molecular diagnosis is expensive.

In addition, although centrifugal microfluidics has many advantages, since the chip generally comprises various reservoirs and interconnected microchannel networks, and a series of complicated pre-treatment processes including sample lysis, sample enrichment, sample washing, sample elution, etc. are required to be performed on the sample before PCR amplification, the pre-treatment process often needs to be performed at a certain temperature, or can be performed most efficiently and rapidly at a certain temperature. And often the temperature required for sample pretreatment is different from the temperature requirement for PCR amplification. The temperature change control of a single temperature zone of a plurality of times of circulation of three-step reaction of PCR reaction, namely high-temperature denaturation, low-temperature annealing and primer extension, can not meet the requirement of a complex PCR amplification system.

Disclosure of Invention

In view of the above, there is a need for a dual temperature zone device for centrifugation and a microfluidic analytical device.

A dual-temperature-zone device for centrifugation is provided with an inner temperature zone and an inner temperature zone opening, wherein the inner temperature zone is communicated with the outside through the inner temperature zone opening; the dual-temperature-zone device is provided with an internal temperature-zone heating assembly in the internal temperature zone; the dual-temperature-zone device is provided with an outer temperature zone and an outer temperature zone opening, and the outer temperature zone is used for being communicated with the outside through the outer temperature zone opening; the double-temperature-zone device is provided with an outer-temperature-zone heating assembly in the outer temperature zone; a heat-insulation isolation structure is arranged between the inner temperature zone and the outer temperature zone, the heat-insulation isolation structure is provided with a groove so that one part of the structure to be centrifuged is accommodated in the inner temperature zone and the other part of the structure to be centrifuged is accommodated in the outer temperature zone, and the heat-insulation isolation structure is used for isolating the air of the inner temperature zone and the air of the outer temperature zone and preventing the heat-insulation isolation structure from blocking the structure to be centrifuged; the outer temperature zone is provided with an outer temperature zone inlet and a sealing cover structure, and the sealing cover structure is used for sealing or opening the outer temperature zone inlet. Above-mentioned dual temperature district device, the design of interior warm area and outer warm area provides the different region of different temperature, is applicable to the centrifugal system to the temperature demand differentiation, is favorable to satisfying different reaction stage to the different demands of temperature on the one hand, and on the other hand is favorable to adapting to more complicated reaction system, to different biochemical reactions, can design the different temperature reaction systems to different temperature rise rates, again on the one hand through the design of outer warm area entry be favorable to realizing the forced air cooling to promote the speed of falling of outer warm area.

Further, in one embodiment, the dual-temperature-zone device further comprises an air duct cylinder, the air duct cylinder is provided with an air duct cylinder cavity and an air duct cylinder inlet, and the air duct cylinder cavity is used for being communicated with the outside through the air duct cylinder inlet; the air duct cylinder cavity is communicated with the outer temperature area through the outer temperature area inlet, and the sealing cover structure is used for sealing or opening the air duct cylinder inlet.

Further, in one embodiment, the dual-temperature-zone device further includes a cold air output device, an output end of the cold air output device is communicated with the air duct cylinder inlet through the sealing cover structure, or an output end of the cold air output device is communicated with the outer temperature-zone inlet through the sealing cover structure.

In one embodiment, the dual-temperature-zone device further comprises a fixing support, the fixing support separates the inner temperature zone into an inner heating zone and an inner chamber which are communicated, and the fixing support further separates the outer temperature zone into an outer heating zone and an outer chamber which are communicated; the inner heating zone is used for accommodating the inner temperature zone heating assembly, and the inner chamber is used for accommodating a part of the structure to be centrifuged; the outer heating zone accommodates the outer temperature zone heating assembly, and the outer chamber is used for accommodating the rest part of the structure to be centrifuged.

In one embodiment, the dual-temperature-zone device is provided with an inner temperature control system and an outer temperature control system; the inner temperature control system is provided with the inner temperature zone and an inner temperature zone opening, and the inner temperature control system is provided with the inner temperature zone heating assembly in the inner temperature zone; the outer temperature control system is provided with the outer temperature zone and an outer temperature zone opening, and an outer temperature zone heating assembly is arranged in the outer temperature zone of the outer temperature control system; the inner temperature control system and the outer temperature control system are arranged in a matched mode, and the heat insulation isolation structure is arranged between the inner temperature control system and the outer temperature control system.

In one embodiment, the dual temperature zone device comprises a centrifugal cover structure and a heating structure; the heating structure is rotationally arranged on the centrifugal cover structure, and the centrifugal cover structure is used for accommodating the structure to be centrifuged; the heating structure is provided with the inner temperature zone opening and the outer temperature zone opening, and the inner temperature zone heating assembly and the outer temperature zone heating assembly are respectively fixed on the heating structure and face the centrifugal cover structure; the centrifugal cover structure is provided with the outer temperature zone inlet and the sealing cover structure; the first isolation part of the heat-insulation isolation structure is fixed on the heating structure, the second isolation part of the heat-insulation isolation structure is fixed on the centrifugal cover structure, and the first isolation part and the second isolation part are matched to form the slot; the side part of the first isolation part facing the rotation center, the side part of the second isolation part facing the rotation center, the heating structure and the centrifugal cover structure jointly enclose to form the inner temperature zone; the side part of the first isolation part, the side part of the second isolation part, the heating structure and the centrifugal cover structure are enclosed together to form the outer temperature zone.

In one embodiment, the heating structure is provided with a cover body, an outer temperature zone first wall part and a fixed bracket; the cover body is provided with the inner temperature zone opening and the outer temperature zone opening; the first isolation part is fixed on the cover body and penetrates through the fixed support, the inner temperature zone heating assembly is positioned on one side of the first isolation part, which faces the rotation center, and the outer temperature zone heating assembly is positioned on one side of the first isolation part, which is far away from the rotation center; the centrifugal cover structure is provided with a cover body, a supporting piece and a second wall part of the outer temperature zone; the cover body is rotatably arranged on the supporting piece, and the supporting piece is fixed on the cover body; the cover body is provided with the outer temperature area inlet and the cover body is provided with the sealing cover structure; said second separator portion being secured to said centrifuge shield structure; the side part of the first isolation part facing the rotation center, the cover body and the fixed support jointly enclose an inner heating area of the inner temperature area, and the inner temperature area heating assemblies are all located in the inner heating area; the side part of the first isolation part, which is far away from the rotation center, the cover body, the first wall part of the outer temperature zone and the fixed support jointly enclose an outer heating zone of the outer temperature zone, and the outer temperature zone heating components are all positioned in the outer heating zone; the side part of the first isolation part facing the rotation center, the side part of the second isolation part facing the rotation center, the fixed support and the cover body jointly enclose an inner chamber of the inner temperature zone, and the inner chamber is used for accommodating a part of a structure to be centrifuged; the side part of the first isolation part, the side part of the second isolation part, the fixing support and the cover body enclose an outer chamber of the outer temperature zone, and the outer chamber is used for accommodating the rest part of the structure to be centrifuged.

In one embodiment, the internal temperature zone heating assembly is fixed on the cover body or the fixed bracket; and/or the outer temperature zone heating assembly is fixed on the cover body or the fixed support.

In one embodiment, the inner temperature zone opening is a one-way vent; and/or the opening of the outer temperature area is a one-way ventilation opening.

In one embodiment, the dual-temperature-zone device further comprises an inner cavity temperature detection assembly, the inner cavity temperature detection assembly is connected with the inner temperature-zone heating assembly, and the inner cavity temperature detection assembly is used for detecting the inner cavity temperature of the inner temperature zone and controlling the inner temperature-zone heating assembly according to the inner cavity temperature; and/or the dual-temperature-zone device further comprises an outer-chamber temperature detection assembly, the outer-chamber temperature detection assembly is connected with the outer-temperature-zone heating assembly, and the outer-chamber temperature detection assembly is used for detecting the outer-chamber temperature of the outer temperature zone and controlling the outer-temperature-zone heating assembly according to the outer-chamber temperature; further, the inner cavity temperature detection assembly is used for detecting the inner cavity temperature of an inner cavity of the inner temperature zone and controlling the inner temperature zone heating assembly according to the inner cavity temperature; and/or the outer cavity temperature detection assembly is used for detecting the outer cavity temperature of the outer cavity of the outer temperature zone and controlling the outer temperature zone heating assembly according to the outer cavity temperature.

In one embodiment, the heat-insulating isolation structure is used for synchronously rotating with the structure to be centrifuged; or the heat-insulating isolation structure and the structure to be centrifuged are arranged in a non-contact manner; or the heat-preservation isolation structure is provided with a sliding part for reducing friction force between the heat-preservation isolation structure and the structure to be centrifuged; the inner temperature zone heating component comprises an inner temperature zone heating module, and further comprises an inner stirring device; the outer temperature zone heating component comprises an outer temperature zone heating module, and further comprises an outer stirring device; furthermore, the dual-temperature-zone device is also provided with a stirring motor, and the stirring motor is positioned outside the inner temperature zone and the outer temperature zone; the stirring motor is respectively connected with the inner stirring device and the outer stirring device; the dual-temperature-zone device is further provided with a rotating shaft for centrifugation and a mounting rack fixed on the rotating shaft, the mounting rack is used for mounting the structure to be centrifuged, and at least part of the mounting rack is located in the internal temperature zone.

A microfluidic analytical device comprising any one of the dual temperature zone devices.

Drawings

Fig. 1 is a schematic structural diagram according to an embodiment of the present application. Fig. 2 is another schematic view of the embodiment shown in fig. 1. FIG. 3 is a schematic sectional view taken along the line A-A of the embodiment shown in FIG. 2. FIG. 4 is a schematic cross-sectional view along the direction B-B of the embodiment shown in FIG. 2. Fig. 5 is a partially enlarged schematic view of the embodiment shown in fig. 4. Fig. 6 is a schematic view of the embodiment of fig. 4 showing the direction of airflow. FIG. 7 is a schematic cross-sectional view along the direction C-C of the embodiment shown in FIG. 2. FIG. 8 is a schematic cross-sectional view taken along the direction D-D of the embodiment shown in FIG. 2. Fig. 9 is another schematic view of the embodiment of fig. 1. Fig. 10 is another schematic view of the embodiment of fig. 1. FIG. 11 is another schematic view of the embodiment of FIG. 1. FIG. 12 is a schematic cross-sectional view taken in the direction E-E of the embodiment shown in FIG. 11. FIG. 13 is another schematic view of the embodiment of FIG. 1.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present application more comprehensible, embodiments accompanying the present application are described in detail below with reference to the accompanying drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present application. This application is capable of embodiments in many different forms than those described herein and that modifications may be made by one skilled in the art without departing from the spirit and scope of the application and it is therefore not intended to be limited to the specific embodiments disclosed below.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only and do not represent the only embodiments.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein in the description of the present application is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

In one embodiment of the application, a dual-temperature-zone device for centrifugation is provided with an inner temperature zone and an inner temperature-zone opening, wherein the inner temperature zone is used for being communicated with the outside through the inner temperature-zone opening; the dual-temperature-zone device is provided with an internal-temperature-zone heating assembly in the internal temperature zone; the dual-temperature-zone device is provided with an outer temperature zone and an outer temperature-zone opening, and the outer temperature zone is communicated with the outside through the outer temperature-zone opening; the double-temperature-zone device is provided with an outer-temperature-zone heating assembly in an outer-temperature zone; a heat insulation isolation structure is arranged between the inner temperature zone and the outer temperature zone, the heat insulation isolation structure is provided with a groove so that one part of the structure to be centrifuged is accommodated in the inner temperature zone and the other part of the structure to be centrifuged is accommodated in the outer temperature zone, and the heat insulation isolation structure is used for isolating the air of the inner temperature zone and the air of the outer temperature zone and preventing the heat insulation isolation structure from blocking the structure to be centrifuged; the outer temperature zone is provided with an outer temperature zone inlet and a sealing cover structure, and the sealing cover structure is used for sealing or opening the outer temperature zone inlet. Above-mentioned dual temperature district device, the design of interior warm area and outer warm area provides the different region of different temperature, is applicable to the centrifugal system to the temperature demand differentiation, is favorable to satisfying different reaction stage to the different demands of temperature on the one hand, and on the other hand is favorable to adapting to more complicated reaction system, to different biochemical reactions, can design the different temperature reaction systems to different temperature rise rates, again on the one hand through the design of outer warm area entry be favorable to realizing the forced air cooling to promote the speed of falling of outer warm area.

In one embodiment, a dual temperature zone device for centrifugation comprises a part of or the whole structure of the following embodiments; that is, the dual temperature zone device includes the following partial technical features or all technical features. In one embodiment, the dual temperature zone device is provided with an inner temperature zone and an outer temperature zone, wherein one part of the structure to be centrifuged is accommodated in the inner temperature zone and the other part is accommodated in the outer temperature zone. For a circular, fan-shaped or circular-or fan-shaped structure to be centrifuged, such as a microfluidic chip, the inner part of the structure to be centrifuged close to the center of rotation is accommodated in the inner temperature zone and the outer part of the structure to be centrifuged far from the center of rotation is accommodated in the outer temperature zone. Further, in one embodiment, the inner temperature zone is formed in a circular shape, and the outer temperature zone is formed in a circular shape. It will be understood that the structure to be centrifuged includes the relevant structure that requires centrifugation, such as a cuvette or a centrifugal microfluidic chip or the like. Such design provides the different region of different temperature, is applicable to in the centrifugal system to the temperature demand differentiation, is favorable to satisfying the different demands to the temperature of different reaction stages.

In one embodiment, the inner temperature zone is used for communicating with the outside through the inner temperature zone openings; the dual-temperature-zone device is provided with an internal-temperature-zone heating assembly in the internal temperature zone; in one embodiment, the inner temperature zone heating assembly comprises an inner temperature zone heating module, and further in one embodiment, the inner temperature zone heating assembly further comprises an inner stirring device; the inner temperature zone heating component comprises an inner temperature zone heating module and an inner stirring device, and the rest of the embodiments are analogized in the same way. In one embodiment, the outer temperature zone is used for communicating with the outside through an outer temperature zone opening; the double-temperature-zone device is provided with an outer-temperature-zone heating assembly in an outer-temperature zone; in one embodiment, the outer temperature zone heating assembly comprises an outer temperature zone heating module, and further, in one embodiment, the inner temperature zone heating module and the outer temperature zone heating module are both circular rings. The inner temperature zone heating module and the outer temperature zone heating module are both heating modules, and only have certain difference in position and shape. The heating module includes, but is not limited to, the following heating methods: heating by a resistance wire device, heating by a heating pipe device or heating by a semiconductor refrigerating sheet device, and the like. Further, in one embodiment, the outer temperature zone heating assembly further comprises an outer stirring device. In one embodiment, the inner temperature zone heating assembly comprises an inner temperature zone heating module and an inner stirring device, the outer temperature zone heating assembly comprises an outer temperature zone heating module and an outer stirring device, and further, the inner stirring device and the outer stirring device are integrally arranged to form a stirring device. Agitating unit, including interior agitating unit and outer agitating unit, its main action includes: rapidly heating; providing a uniform temperature field; can provide a uniform temperature field with the requirement of +/-0.3 ℃ or even higher. When the inner temperature zone heating assembly further comprises an inner stirring device and/or the outer temperature zone heating assembly further comprises an outer stirring device, further, the dual-temperature zone device is also provided with a stirring motor which is positioned outside the inner temperature zone and the outer temperature zone; the stirring motor is respectively connected with the inner stirring device and/or the outer stirring device; when the inner temperature zone heating assembly further comprises an inner stirring device and the outer temperature zone heating assembly further comprises an outer stirring device, the stirring motor is respectively connected with the inner stirring device and the outer stirring device; the rest of the examples are analogized. The stirring motor is used for respectively driving the inner stirring device and/or the outer stirring device to rotate so as to improve the temperature rising and falling speed of the inner temperature area and/or the outer temperature area. When the internal temperature zone heating module and/or the external temperature zone heating module work, the stirring motor respectively drives the internal stirring device and/or the external stirring device to rotate so as to increase the heating rate, quickly reach the target temperature set by the chamber and enable the air in the whole internal/external chamber to be in a stable target temperature field; when the internal temperature zone heating module and/or the external temperature zone heating module do not work, the stirring motor drives the internal stirring device and/or the external stirring device to rotate respectively so as to increase the cooling rate. This embodiment embodies two important points of the invention: firstly, the dual-temperature area is divided, and the mutual influence between the inner chamber and the outer chamber is avoided; secondly, through stirring and realization thereof, the heating, ventilation and heat dissipation functions are improved, and the temperature uniformity of the dual-temperature area is realized.

In one embodiment, a heat preservation isolation structure is arranged between the inner temperature zone and the outer temperature zone, and mainly plays a role in heat preservation and heat insulation, and the heat preservation isolation structure has both isolation and heat preservation and heat insulation effects. The heat-insulation isolation structure is provided with a slot so that one part of the structure to be centrifuged is accommodated in the inner temperature zone and the other part of the structure to be centrifuged is accommodated in the outer temperature zone, and the heat-insulation isolation structure is used for isolating the air in the inner temperature zone and the air in the outer temperature zone and preventing the heat-insulation isolation structure from blocking the structure to be centrifuged; that is, the heat-insulating isolation structure forms a structure in which the inner temperature zone and the outer temperature zone are relatively isolated, and can be absolutely sealed or not, so that the dual-temperature-zone device can be loaded into a structure to be centrifuged, which needs centrifugal treatment, a part of the structure to be centrifuged is located in the inner temperature zone, and the other part of the structure to be centrifuged is located in the outer temperature zone, and the inner temperature zone and the outer temperature zone are isolated by the heat-insulating isolation structure, and the heat-insulating isolation structure does not form an obstacle to the rotation of the structure to be centrifuged, namely, does not influence the centrifugation of the structure to be centrifuged. The slotting is to form certain 'airflow obstruction' so as to achieve the aim that the air in the inner chamber and the air in the outer chamber are not communicated with each other and form relatively independent temperature areas. Further, in one embodiment, the heat-insulating isolation structure is used for synchronously rotating with the structure to be centrifuged, and the heat-insulating isolation structure can form absolute seal relative to the inner temperature zone and the outer temperature zone; or the heat-insulating isolation structure and the structure to be centrifuged are arranged in a non-contact manner; or, the heat-insulating isolation structure is provided with a sliding part for reducing friction force between the heat-insulating isolation structure and the structure to be centrifuged, and at the moment, the heat-insulating isolation structure can form relative sealing between the inner temperature zone and the outer temperature zone, namely, the 'airflow obstruction' is formed. The design is favorable for isolating the inner temperature zone and the outer temperature zone through the heat-insulating isolation structure without influencing the rotation of the structure to be centrifuged, thereby being favorable for adapting to more complex reaction systems, and different temperature reaction systems aiming at different temperature rise rates can be designed aiming at different biochemical reactions.

In one embodiment, the outer temperature zone has an outer temperature zone inlet and a cover structure for closing or opening the outer temperature zone inlet. Further, in one embodiment, the dual temperature zone device further includes an electronic control module for controlling the cover structure to close or open the inlet of the external temperature zone. Further, in one embodiment, the dual-temperature-zone device further comprises an air temperature adjusting device, and an output end of the air temperature adjusting device is communicated with the outer temperature-zone inlet through the cover structure. According to the design, the air cooling and cooling can be controlled and realized through the effective switch control of the inlet of the outer temperature area, so that the temperature reduction rate of the outer temperature area is improved, and warm air can be input through the inlet of the outer temperature area to improve the temperature rise rate of the outer temperature area when necessary.

Further, in one embodiment, the dual-temperature-zone device further comprises an air duct cylinder, the air duct cylinder is provided with an air duct cylinder cavity and an air duct cylinder inlet, and the air duct cylinder cavity is used for being communicated with the outside through the air duct cylinder inlet; the air duct cylinder cavity is communicated with the external temperature zone through the external temperature zone inlet, and the sealing cover structure is used for sealing or opening the air duct cylinder inlet. Further, in one embodiment, the dual-temperature-zone device further comprises a cold air output device, an output end of the cold air output device is communicated with the air duct cylinder inlet through the sealing cover structure, or an output end of the cold air output device is communicated with the outer temperature-zone inlet through the sealing cover structure. Further, in one embodiment, the dual-temperature-zone device further comprises an air temperature adjusting device, and an output end of the air temperature adjusting device is communicated with the air duct cylinder inlet through a sealing cover structure. The design is favorable for controlling the air inlet state of the outer temperature area, is matched with the realization of the control of the temperature rise rate and the temperature drop rate, and is particularly suitable for a centrifugal micro-fluidic structure or a nucleic acid analysis system which needs to carry out PCR reaction.

In one embodiment, the dual-temperature-zone device further comprises a fixing bracket, the fixing bracket separates the inner temperature zone into an inner heating zone and an inner chamber which are communicated, and the fixing bracket also separates the outer temperature zone into an outer heating zone and an outer chamber which are communicated; the inner heating zone is used for accommodating the inner temperature zone heating assembly, and the inner chamber is used for accommodating a part of the structure to be centrifuged; the outer heating zone accommodates the outer temperature zone heating assembly, and the outer chamber is used for accommodating the rest part of the structure to be centrifuged. Further, in one embodiment, the fixation bracket is fixed inside the dual temperature zone device. Further, in one of the embodiments, the fixing bracket has a lattice shape or a mesh shape. The fixed support is used for heating positions at intervals and enabling a structure to be centrifuged, so that the centrifugation result or the analysis result is prevented from being influenced.

In one embodiment, the dual-temperature-zone device is provided with an inner temperature control system and an outer temperature control system; the internal temperature control system is provided with an internal temperature zone and an internal temperature zone opening, and an internal temperature zone heating assembly is arranged in the internal temperature zone of the internal temperature control system; the outer temperature control system is provided with an outer temperature zone and an outer temperature zone opening, and an outer temperature zone heating component is arranged in the outer temperature zone of the outer temperature control system; the inner temperature control system and the outer temperature control system are arranged in a matched mode, and a heat preservation isolation structure is arranged between the inner temperature control system and the outer temperature control system. Further, in one embodiment, the dual-temperature-zone device further comprises a fixing support, the fixing support separates the inner temperature zone into an inner heating zone and an inner chamber which are communicated, and the fixing support also separates the outer temperature zone into an outer heating zone and an outer chamber which are communicated; the inner heating zone is used for accommodating the inner temperature zone heating assembly, and the inner chamber is used for accommodating a part of the structure to be centrifuged; the outer heating zone accommodates the outer temperature zone heating assembly, and the outer chamber is used for accommodating the rest part of the structure to be centrifuged. The rest of the examples are analogized. The design is favorable for adapting to more complex reaction systems, and different temperature reaction systems aiming at different temperature rise rates can be designed aiming at different biochemical reactions.

In one embodiment, the dual temperature zone device comprises a centrifugal cover structure and a heating structure; the heating structure is rotationally arranged on the centrifugal cover structure, and the centrifugal cover structure is used for accommodating a structure to be centrifuged; the heating structure is provided with an inner temperature zone opening and an outer temperature zone opening, and the inner temperature zone heating assembly and the outer temperature zone heating assembly are respectively fixed on the heating structure and face the centrifugal cover structure; the centrifugal cover structure is provided with an outer temperature area inlet and is provided with a sealing cover structure; a first isolation part of the heat-insulation isolation structure is fixed on the heating structure, a second isolation part of the heat-insulation isolation structure is fixed on the centrifugal cover structure, and the first isolation part and the second isolation part are matched to form a slot; the side part of the first isolation part facing the rotation center, the side part of the second isolation part facing the rotation center, the heating structure and the centrifugal cover structure jointly enclose to form an inner temperature zone; the side part of the first isolation part far away from the rotation center, the side part of the second isolation part far away from the rotation center, the heating structure and the centrifugal cover structure enclose together to form an outer temperature zone. In one embodiment, the heating structure is provided with a cover body, a first wall part of the outer temperature zone and a fixed bracket; the cover body is provided with an inner temperature zone opening and an outer temperature zone opening; the first isolation part is fixed on the cover body and penetrates through the fixing support, the inner temperature zone heating assembly is positioned on one side of the first isolation part, which faces the rotation center, and the outer temperature zone heating assembly is positioned on one side of the first isolation part, which is far away from the rotation center; the centrifugal cover structure is provided with a cover body, a supporting piece and a second wall part of the outer temperature zone; the cover body is rotationally arranged on the supporting piece, and the supporting piece is fixed on the cover body; the cover body is provided with an external temperature area inlet and is provided with a sealing cover structure; the second isolation part is fixed on the centrifugal cover structure; the side part of the first isolation part facing the rotation center, the cover body and the fixed support jointly enclose an inner heating area of an inner temperature area, and the inner temperature area heating assemblies are all positioned in the inner heating area; the side part of the first isolation part, which is far away from the rotation center, the cover body, the first wall part of the outer temperature zone and the fixed support enclose an outer heating zone of the outer temperature zone together, and the outer temperature zone heating assemblies are all positioned in the outer heating zone; the side part of the first isolation part facing the rotation center, the side part of the second isolation part facing the rotation center, the fixing support and the cover body jointly enclose an inner chamber of the inner temperature zone, and the inner chamber is used for accommodating a part of a structure to be centrifuged; the side part of the first isolation part, the side part of the second isolation part and the cover body enclose an outer chamber of an outer temperature zone, and the outer chamber is used for accommodating the rest parts of the structure to be centrifuged. Further, in one embodiment, the heating structure is rotatably disposed on the centrifugal cover structure through a rotating connecting member, the dual-temperature-zone device further includes a fixing member, such as a screw member or a buckle member, for rotatably fixing the heating structure on the centrifugal cover structure, and the fixing member and the rotating connecting member are respectively located on two opposite sides of the centrifugal cover structure. When the heating structure rotates to a certain position relative to the centrifugal cover structure, the first wall part of the outer temperature zone is contacted with the second wall part of the outer temperature zone, and the heating structure is rotationally fixed on the centrifugal cover structure to form a centrifugal environment. Further, in one embodiment, a deformation sealing layer is disposed between the first wall portion of the outer temperature zone and the second wall portion of the outer temperature zone, and is used for deforming when the heating structure is rotatably fixed on the centrifugal cover structure, so as to achieve a better sealing effect. Further, in one embodiment, the cover body is provided with a motor through hole, the dual-temperature-zone device is further provided with a stirring motor, and the stirring motor is positioned outside the heating structure and the centrifugal cover structure; the output end of the stirring motor penetrates through the motor through opening and is respectively connected with the inner stirring device and the outer stirring device. In one embodiment, the internal temperature zone heating assembly is fixed on the cover body or the fixed bracket; and/or the heating component of the outer temperature zone is fixed on the cover body or the fixed bracket. By the design, the centrifugal cover structure and the heating structure form two relatively independent parts, and the centrifugal cover structure is easy to open or close, convenient to place or replace the structure to be centrifuged and more convenient to use.

In one embodiment, the inner temperature zone opening and/or the outer temperature zone opening comprises a ventilation mesh, a ventilation grid or a ventilation groove, and the ventilation mesh, the ventilation grid or the ventilation groove is used for ventilation, so that the equipment is prevented from being damaged by expansion and contraction during heating. In one embodiment, the opening of the internal temperature zone is a one-way ventilation opening; or the opening of the outer temperature area is a one-way ventilation opening. In one embodiment, the opening of the internal temperature zone is a one-way ventilation opening; and the opening of the outer temperature area is a one-way ventilation opening. The rest of the examples are analogized. Further, in one embodiment, the inner temperature zone opening and the outer temperature zone opening are located on one side surface of the dual temperature zone device, and the outer temperature zone inlet is located on the other side surface of the dual temperature zone device opposite to the side surface. Due to the design, the heat-insulating isolation structure does not completely seal the inner temperature area and the outer temperature area and is matched with the inlet of the outer temperature area, so that the control of the direction of the airflow is facilitated, and the airflow enters from the inlet of the outer temperature area and flows out from the opening of the inner temperature area and the opening of the outer temperature area.

In one embodiment, the dual-temperature-zone device further comprises an inner-chamber temperature detection assembly, the inner-chamber temperature detection assembly is connected with the inner-temperature-zone heating assembly, and the inner-chamber temperature detection assembly is used for detecting the inner chamber temperature of the inner temperature zone and controlling the inner-temperature-zone heating assembly according to the inner chamber temperature; and/or the dual-temperature-zone device also comprises an outer-cavity temperature detection assembly, the outer-cavity temperature detection assembly is connected with the outer-temperature-zone heating assembly, and the outer-cavity temperature detection assembly is used for detecting the outer-cavity temperature of the outer temperature zone and controlling the outer-temperature-zone heating assembly according to the outer-cavity temperature; further, the inner cavity temperature detection assembly is used for detecting the inner cavity temperature of the inner cavity of the inner temperature zone and controlling the heating assembly of the inner temperature zone according to the inner cavity temperature; and/or the outer cavity temperature detection assembly is used for detecting the outer cavity temperature of the outer cavity of the outer temperature zone and controlling the outer temperature zone heating assembly according to the outer cavity temperature. It can be understood that the temperature of the inner cavity is the temperature of the inner temperature zone, and the temperature of the outer cavity is the temperature of the outer temperature zone; the inner cavity temperature and the outer cavity temperature are used for differentiation. The design is matched with other embodiments, so that the accurate control of the temperature of the inner temperature area and the temperature of the outer temperature area can be realized, the more complex reaction system can be adapted, and different temperature reaction systems aiming at different temperature rise rates can be designed aiming at different biochemical reactions.

In one embodiment, the dual-temperature-zone device is further provided with a rotating shaft for centrifugation and a mounting rack fixed on the rotating shaft, the mounting rack is used for mounting a structure to be centrifuged, and the mounting rack is at least partially positioned in the internal temperature zone. In one embodiment, the heat-insulating isolation structure is used for synchronously rotating with the structure to be centrifuged; or the heat-insulating isolation structure and the structure to be centrifuged are arranged in a non-contact manner; or the heat-insulating isolation structure is provided with a sliding part used for reducing friction force between the heat-insulating isolation structure and the structure to be centrifuged; the inner temperature zone heating assembly comprises an inner temperature zone heating module, and further comprises an inner stirring device, namely the inner temperature zone heating assembly comprises an inner temperature zone heating module and an inner stirring device; the outer temperature zone heating assembly comprises an outer temperature zone heating module, and further comprises an outer stirring device; furthermore, the double-temperature-zone device is also provided with a stirring motor, and the stirring motor is positioned outside the inner temperature zone and the outer temperature zone; the stirring motor is respectively connected with the inner stirring device and the outer stirring device; the dual-temperature-zone device is further provided with a rotating shaft for centrifugation and a mounting rack fixed on the rotating shaft, the mounting rack is used for mounting a structure to be centrifuged, and at least part of the mounting rack is located in the internal temperature zone. By the design, related parts with the centrifugal function can be integrally designed in the dual-temperature-zone device, so that the functions of the dual-temperature-zone device are enriched, and a user does not need to assemble accessories by himself to achieve the technical effect of buying and using the dual-temperature-zone device.

In one embodiment, as shown in fig. 2 and 4, a dual-temperature zone device for centrifugation is provided with an internal temperature zone 111 and an internal temperature zone opening 113, wherein the internal temperature zone 111 is used for being communicated with the outside through the internal temperature zone opening 113 so as to naturally cool the internal temperature zone; the double-temperature zone is arranged in the inner-temperature zone 111 and is provided with an inner-temperature zone heating component 888; the dual-temperature-zone device is provided with an outer temperature zone 140 and an outer temperature zone opening 190, and the outer temperature zone is communicated with the outside through the outer temperature zone opening so as to naturally cool the outer temperature zone; the dual-temperature zone device is provided with an external-temperature-zone heating assembly 999 in an external-temperature zone 140; a heat-insulating isolation structure 115 is arranged between the inner temperature zone and the outer temperature zone, the heat-insulating isolation structure 115 is provided with a groove so that one part of the structure to be centrifuged is accommodated in the inner temperature zone 111 and the other part of the structure to be centrifuged is accommodated in the outer temperature zone 140, and the heat-insulating isolation structure 115 is used for synchronously rotating with the structure to be centrifuged 117 so as to prevent the heat-insulating isolation structure 115 from forming obstruction relative to the structure to be centrifuged; the outer temperature zone 140 is provided with an outer temperature zone inlet 130 and a cover structure 123, and the outer temperature zone inlet is used for uniformly entering the outer temperature zone after cooling air is split; the cover structure 123 is used to close or open the external temperature area entrance 130. In this embodiment, referring to fig. 9, the dual-temperature-zone device further includes an air duct tube 121, which is used for cooling the intake air, storing the air, and shunting the air; the air duct cylinder is provided with an air duct cylinder cavity 120 and an air duct cylinder inlet 122, and the air duct cylinder cavity is communicated with the outside through the air duct cylinder inlet 122; the air duct cylinder inlet is used for air to enter the air duct cylinder cavity, the air duct cylinder cavity is communicated with the external temperature zone through the external temperature zone inlet, and the sealing cover structure is used for sealing or opening the air duct cylinder inlet. In this embodiment, referring to fig. 5, the dual-temperature-zone apparatus further includes a fixing bracket 118, the fixing bracket 118 separates the inner temperature zone 111 into an inner heating zone 126 and an inner chamber 127 which are communicated with each other, and the fixing bracket 118 separates the outer temperature zone 140 into an outer heating zone 128 and an outer chamber 129 which are communicated with each other; referring to fig. 7 and 8, the inner temperature zone 111 includes an inner heating zone 126 and an inner chamber 127, and the outer temperature zone 140 includes an outer heating zone 128 and an outer chamber 129; the inner heating zone 126 accommodates an inner temperature zone heating assembly, and the inner chamber 127 is used for accommodating a part of the structure 117 to be centrifuged; the outer heating zone 128 houses the outer warm zone heating assembly and the outer chamber 129 is used to house the rest of the structure 117 to be centrifuged. The internal temperature zone heating component comprises an internal temperature zone heating module 110 and an internal stirring device 114; the external temperature zone heating component comprises an external temperature zone heating module 180 and an external stirring device 170. The inner temperature zone heating module is used for providing an inner temperature zone heat source in a controlled mode, the outer temperature zone heating module is used for providing an outer temperature zone heat source in a controlled mode, the inner stirring device is used for stirring air in the inner temperature zone, and the outer stirring device is used for stirring air in the outer temperature zone to enable the air temperature to be uniform. The outer temperature zone heating module outer temperature zone wall 150 has an outer temperature zone first wall portion 303 and an outer temperature zone second wall portion 302, which serve as an outer temperature zone support member. Further, the inner wall of the outer temperature zone wall 150 is further provided with a heat preservation and insulation material layer 160 for preserving and insulating heat in the outer temperature zone. In this embodiment, referring to fig. 6, the rotation direction 212 of the stirring motor 112 is the same as the rotation direction 213 of the rotating shaft 119, but may be different in practical application; the air three-dimensional circulation is illustrated as follows: in the cooling stage, when the cover structure 123 is opened, the first airflow 201 enters the air duct cylinder cavity 120 from the air duct cylinder inlet 122, the formed second airflow 202 and third airflow 203 enter the outer chamber 129 of the outer temperature zone 140 through the outer temperature zone inlet 130, the formed fourth airflow 204 and fifth airflow 205 enter the outer heating zone 128 of the outer temperature zone 140 after the centrifugal structure is cooled, and the formed sixth airflow 206 leaves the outer temperature zone 140 through the outer temperature zone opening 190; at this time, a temperature difference is formed between the inner temperature zone and the outer temperature zone due to the existence of the heat insulating structure 115. In the temperature raising stage, the gas heated by the internal temperature zone heating module 110 forms a seventh gas flow 207 and an eighth gas flow 208, enters the inner chamber 127 of the internal temperature zone 111 from the internal heating zone 126 of the internal temperature zone 111 through the fixed support 118, heats the inner circle part of the structure to be centrifuged close to the rotation center, and returns the heated ninth gas flow 209 to the internal heating zone 126 through the fixed support 118 for further heating; the gas heated by the external temperature zone heating module 180 forms a tenth gas flow 210 and an eleventh gas flow 211, enters the outer chamber 129 of the external temperature zone 140 from the external heating zone 128 of the external temperature zone 140 through the fixing support 118, heats the outer ring part of the structure to be centrifuged, which is far away from the rotation center, and the fourth gas flow 204 and the fifth gas flow 205 formed after heating flow back to the external heating zone 128 through the fixing support 118 for further heating; at this time, due to the existence of the heat insulation isolation structure 115, a temperature difference is formed between the inner temperature zone and the outer temperature zone; and due to the existence of the cover structure 123, the airflow is not easy to escape from the outer temperature zone opening 190 or the inner temperature zone opening 113, and the outer temperature zone opening 190 or the inner temperature zone opening 113 also plays a certain role in exhausting air and avoiding expansion. In practice there may be a plurality of second 202, third 203, fourth 204, fifth 205, seventh 207, eighth 208, ninth 209, tenth 210 and eleventh 211 air streams. And (3) cooling the internal temperature zone: and naturally cooling the inner temperature zone through the opening of the inner temperature zone. And (3) cooling the outer temperature zone: the cold air is sent to the inlet of the air duct cylinder through the fan and the air duct and reaches the inlet of the outer temperature zone through the air duct cylinder, wherein the inlets of the outer temperature zone are circumferentially and uniformly distributed and enter the outer temperature zone, and after heat and cold exchange, high-temperature gas is discharged from the opening of the outer temperature zone, so that the temperature drop function of the outer temperature zone is achieved. Due to the design, the two temperature areas can effectively form respective air fluid circulation, the respective temperature uniformity of the two temperature areas is ensured, the two temperature areas are subjected to special treatment due to the existence of the heat insulation mechanism and the gap between the two temperature areas, for example, the heat insulation material adopts the convex groove to carry out maximum limitation, and the temperatures of the two temperature areas can be respectively and independently and effectively controlled. In one embodiment, the two temperature zone separation means are integrated in a stationary support, for example a stationary plate, which is in turn connected to the outer temperature zone wall of the heating structure. The strength of the structure is guaranteed, and on the premise of fixation, the design concept of driving the stirring impellers of two temperature areas to stir air by one stirring motor and the realization of the design concept are met.

In one embodiment, as shown in fig. 10, the dual temperature zone device comprises a centrifuge shield structure 200 and a heating structure 100; the heating structure is rotationally arranged on the centrifugal cover structure, and the centrifugal cover structure is used for accommodating a structure to be centrifuged; as shown in fig. 1, 2 and 3, the heating structure includes a cover 304, a first wall 303 of the outer temperature range, and a fixing bracket 118; the heating structure is provided with an inner temperature zone opening 113 and an outer temperature zone opening 190, and an inner temperature zone heating component 888 and an outer temperature zone heating component 999 are respectively fixed on the heating structure and face the centrifugal cover structure; the centrifugal cover structure is provided with an outer temperature area inlet 130 and a sealing cover structure 123 is arranged at the air duct cylinder inlet 122 of the air duct cylinder 121; referring to fig. 5, the insulation structure 115 is used to isolate the inner and outer temperature zones to form two relatively independent spaces, the first insulation part 124 of the insulation structure is fixed on the heating structure, the second insulation part 125 of the insulation structure is fixed on the centrifugal cover structure, and the first insulation part and the second insulation part cooperate to form a slot; the side of the first isolation part 124 facing the rotation center, the side of the second isolation part 125 facing the rotation center, the heating structure and the centrifugal cover structure together enclose an inner temperature zone 111; the side of the first isolation portion far from the rotation center, the side of the second isolation portion far from the rotation center, the heating structure and the centrifugal cover structure enclose together to form an outer temperature zone 140. The cover 304 is provided with an inner temperature zone opening 113 and an outer temperature zone opening 190; the first isolation part 124 is fixed on the cover 304 and penetrates through the fixing support 118, the inner temperature zone heating assembly is positioned on one side of the first isolation part 124 facing the rotation center, and the outer temperature zone heating assembly is positioned on one side of the first isolation part 124 far away from the rotation center; the centrifugal cover structure is provided with a cover body 301, a supporting piece 305 and an outer temperature zone second wall part 302; the cover 304 is rotatably disposed on the supporting member 305 through a rotating connector 313, and the supporting member 305 is fixed on the cover 301 for providing a rotating supporting point for the heating structure; the cover body 301 is provided with an external temperature area inlet 130 and the cover body 301 is provided with a sealing cover structure; the second separator portion 125 is secured to the centrifuge shield structure; the side part of the first isolation part 124 facing the rotation center, the cover body 304 and the fixing bracket 118 together enclose an inner heating zone 126 of the inner temperature zone 111, and the inner temperature zone heating components are all located in the inner heating zone 126; the side part of the first isolation part 124 far away from the rotation center, the cover body 304, the outer temperature zone first wall part 303 and the fixing support 118 together enclose an outer heating zone 128 of the outer temperature zone 140, and the outer temperature zone heating components are all located in the outer heating zone 128; the side part of the first isolation part 124 facing the rotation center, the side part of the second isolation part 125 facing the rotation center, the fixing bracket 118 and the cover body 301 jointly enclose an inner chamber 127 of the inner temperature zone 111, and the inner chamber 127 is used for accommodating a part of a structure to be centrifuged; the side of the first isolation part 124 far from the rotation center, the side of the second isolation part 125 far from the rotation center, the fixing bracket 118 and the cover 301 together enclose an outer chamber 129 forming an outer temperature zone 140, and the outer chamber 129 is used for accommodating the rest of the structure to be centrifuged. The cover body is a centrifugal cover bottom plate, and the cover body is a heating structure cover plate. In this embodiment, the cover 304 has at least one first fixing screw hole 307, at least one second fixing screw hole 310, etc. the first fixing screw hole is used to fix the related structure of the heating structure, such as the internal temperature heating module, etc.; the second fixing screw hole is used for fixing the centrifugal cover structure and the heating structure mutually, and separation after an inner temperature zone and an outer temperature zone are formed by closing is prevented. As shown in fig. 11, 12 and 13, the first end 308 and the second end 309 of the fixing bracket protrude from the first wall of the outer temperature range and the cover, which is advantageous for mounting the fixing bracket. The fixing bracket is further provided with a fixing strip 312 for supporting. In this embodiment, the cover 301 is provided with a mounting hole 306 for fixing the whole dual-temperature-zone device through the mounting hole. The number of mounting holes 306 is at least 3. In this embodiment, the inner stirring device is further provided with an inner stirring kit 315.

In one embodiment, as shown in fig. 4, the dual-temperature-zone device is provided with an inner temperature control system and an outer temperature control system; the internal temperature control system is provided with an internal temperature zone 111 and an internal temperature zone opening 113, and an internal temperature zone heating assembly is arranged in the internal temperature zone 111 of the internal temperature control system; the outer temperature control system is provided with an outer temperature zone 140 and an outer temperature zone opening 190, and the outer temperature control system is provided with an outer temperature zone heating component in the outer temperature zone 140; the inner temperature control system and the outer temperature control system are arranged in a matched mode, and a heat preservation isolation structure 115 is arranged between the inner temperature control system and the outer temperature control system. In this embodiment, the dual-temperature-zone device is further provided with a stirring motor 112, and the stirring motor 112 is located outside the inner temperature zone and the outer temperature zone; the stirring motor 112 is respectively connected with the inner stirring device 114 and the outer stirring device 170 and is used for providing power for the stirring devices of the inner and outer temperature zones; thus, the stirring devices of the inner and outer temperature areas are driven by a stirring motor to drive the stirring devices of the respective temperature areas to rotate at high speed, and the stirring devices of the respective temperature areas stir the air of the different temperature areas respectively to meet the uniformity requirement of the air in the respective temperature areas. In this embodiment, the dual temperature zone device further includes a rotating shaft 119 for centrifugation and a mounting bracket 191 fixed to the rotating shaft, the mounting bracket 191 is used for mounting the structure 117 to be centrifuged, and the mounting bracket 191 is at least partially located in the internal temperature zone 111. The rotating shaft is used for being connected with a power output device so as to transmit centrifugal force to the structure to be centrifuged. Furthermore, the structure to be centrifuged is further provided with an isolation mechanism 116 for isolating the inner temperature zone and the outer temperature zone in cooperation with a heat insulation isolation structure 115 or a first isolation part 124 and a second isolation part 125 thereof. The cover 301 further has at least one functional module interface or optical system interface 311.

In one embodiment, a microfluidic analytical device includes the dual temperature zone device of any of the embodiments. In one embodiment, the microfluidic analytical device further comprises a structure to be centrifuged; in one embodiment, the structure to be centrifuged is a microfluidic chip. In one embodiment, the microfluidic analysis device, also called a centrifugal microfluidic full-automatic nucleic acid analysis device with dual temperature zones, can be matched with a corresponding centrifugal microfluidic chip to realize full automation of a full-extraction molecular diagnosis project, so that molecular diagnosis becomes faster, the operation is simpler and the device does not depend on a molecular diagnosis laboratory any more. The design forms a novel centrifugal micro-fluidic full-automatic nucleic acid analysis device temperature control system with double temperature zones on the traditional centrifugal micro-fluidic PCR system, divides the space where the centrifugal micro-fluidic chip is located into two relatively independent temperature zones, and meets different temperature requirements of the centrifugal micro-fluidic full-automatic nucleic acid analysis device with the double temperature zones on different reaction stages. In one embodiment, the microfluidic analysis device comprises a centrifugal platform driven by a motor, an inner temperature control system and an outer temperature control system; wherein, the centrifugal micro-fluidic chip is combined with the inner temperature zone and the outer temperature zone to form two temperature control systems with relatively independent inner and outer temperature zones. The internal temperature control system is provided with an internal temperature zone heating module, an internal temperature zone temperature detection module, an internal chamber temperature reduction module, an internal temperature zone air stirring module and the like; the internal temperature zone heating module is mainly used for heating the internal chamber, the internal chamber temperature reduction module is mainly used for reducing the temperature of the internal chamber, and the internal temperature zone air stirring module is mainly used for stirring the air of the internal temperature zone so as to enable the temperature of the internal temperature zone to meet the requirement of uniformity. The outer temperature control system is provided with an outer temperature zone heating module, an outer chamber temperature detection module, an outer chamber temperature reduction module, an outer temperature zone air stirring module and the like; the two independent temperature control systems can independently control the heating modules and the temperature reduction modules in the systems so as to realize temperature rise or temperature reduction in the temperature zone. The outer temperature zone heating module is mainly used for heating the outer chamber, the outer chamber temperature reduction module is mainly used for cooling the outer chamber, and the outer temperature zone air stirring module is mainly used for stirring air in the outer temperature zone, so that the temperature of the outer temperature zone meets the uniformity requirement. In one embodiment, the centrifugal microfluidic chip is driven by a motor to rotate in the inner and outer temperature zones. The two temperature zones are temperature control systems with respective independent temperature control. The temperature control system is provided with a cavity heating module, a temperature detection module, a cavity temperature reduction module, an air stirring module and the like, and can respectively realize rapid temperature rise and rapid temperature reduction.

Examples of specific applications continue to be given below. In certain molecular diagnostic projects, there is a need for the following techniques: on the one hand, pyrolysis is required, typically at 95 ℃. On the other hand, it is desirable to hydrolyze proteins in proteinase K to prevent them from inhibiting the subsequent PCR reaction, and the typical temperature for hydrolyzing proteins is 60-65 ℃. On the other hand, presetting and releasing of a liquid reagent in the microfluidic chip are required, and in some application examples, presetting and releasing are realized by heating and melting a phase-change material at one end of a liquid storage container; in some application examples, the microfluidic chip needs to integrate phase change valves to better realize the manipulation of liquid, and the opening of the phase change valves needs temperature control. In another aspect, a typical PCR reaction requires three temperature cycles, and the temperature precision, uniformity and temperature increase and decrease rate are high. Therefore, the temperature control requirement of the PCR reaction is obviously different from other items, and the temperature increase and decrease rate of the PCR reaction needs to be increased to increase the efficiency of the PCR reaction, so that the PCR reaction area needs to be independent, and the heat capacity of the temperature control area is reduced; the temperature control precision and uniformity of the PCR reaction zone are higher, the cooling rate is also higher, and a cooling device is needed; other items are usually completed before the PCR reaction, so the PCR reaction is the final step, and other temperature control processes are preferably independent from the PCR reaction to prevent interference. Therefore, with the dual-temperature-zone device or the microfluidic analysis device according to each of the above embodiments, different temperature requirements of the nucleic acid pretreatment and the PCR amplification reaction in the full-automatic nucleic acid analysis device are met, so as to realize the full automation of the nucleic acid analysis device. Secondly, the centrifugal micro-fluidic chip is divided into two relatively independent temperature zones so as to meet different requirements of different reaction stages on temperature. And thirdly, the double-temperature zone can adapt to a more complex reaction system, different temperature reaction systems aiming at different temperature rise rates can be designed aiming at different biochemical reactions, the centrifugal micro-fluidic system is more likely to be used for PCR pretreatment, and different temperature requirements of the centrifugal micro-fluidic full-automatic nucleic acid analysis device of the double-temperature zone on different reaction stages are met. The PCR amplification cavity is separated into an external temperature area, and the temperature control of the external temperature area is easier to realize; the PCR amplification cavity is separated out of the external temperature zone, so that the temperature rise rate and the temperature drop rate of liquid in the PCR temperature zone can be greatly improved, and the PCR reaction time is shortened; and the PCR amplification cavity is separated from an external temperature zone, and the PCR amplification cavity is amplified by adopting one temperature zone, so that the temperature control precision is higher, the required heating power is lower, the heating time under the same power is greatly shortened, the resistance wire power can be greatly reduced, the running power of the whole instrument is further reduced, and the energy consumption is reduced. Finally, the fault tolerance rate of the centrifugal microfluidic full-automatic nucleic acid analysis device with the double temperature zones is increased, the inner temperature zone can accommodate a more complex reaction system and a microfluidic pipeline without prolonging the PCR amplification time, the presetting and releasing of a liquid reagent can be realized in a microfluidic chip without influencing the PCR amplification system, the cycle time of the PCR amplification is greatly shortened, and the possibility of small size and rapidness is provided for an in-vitro diagnostic instrument.

For a dual-temperature-zone device or a microfluidic analysis device, the working principle of temperature control of an internal temperature zone, such as an internal temperature control system, is simply explained as follows: the detection sample reagent is added into the centrifugal microfluidic chip, and the sample reagent is subjected to sample treatment when the inner cavity passes through various complex pipelines through the combination of centrifugal force and temperature change, wherein the sample treatment comprises but is not limited to the processes of sample separation, sample enrichment, sample cracking, sample extraction, sample cleaning and the like. Pyrolysis (typically 95 ℃) is required in certain molecular diagnostic projects; hydrolysis of proteins in proteinase K is required in certain molecular diagnostic projects to prevent these proteins from inhibiting subsequent PCR reactions, and the typical temperature for hydrolysis of proteins is 60-65 ℃; presetting and releasing of a liquid reagent in a microfluidic chip are required to be realized, and in some application examples, the presetting and releasing are realized by heating and melting a phase-change material at one end of a liquid storage container; in some application examples, the microfluidic chip needs to integrate phase change valves to better realize the manipulation of liquid, and the opening of the phase change valves needs temperature control. The temperature uniformity of the inner cavity is mainly completed by a stirring module, and the stirring module drives a stirring impeller to rotate at a high speed by a motor so as to ensure that the air in the cavity exchanges heat at a high speed and meets the requirement of temperature uniformity; the temperature change control of the inner chamber is mainly completed through the assistance of the heating module and the cooling module, when the temperature of the inner chamber is detected to be lower than a target temperature value (60-65 ℃), the inner chamber temperature control system enables the heating module to work to emit heat, the cooling module does not work to enable the overall temperature of the inner chamber to rise, when the temperature is detected to be higher than the target value or higher than the target value (60-65 ℃), the inner chamber temperature control system enables the cooling module to work, and the temperature cooling module does not work until the overall temperature of the inner chamber reaches the target value. The working principle of the temperature control of the outer temperature zone, such as the outer temperature control system, is simply explained as follows: after a series of sample treatments are carried out on the sample reagent in the centrifugal microfluidic chip, the sample reagent flows into the PCR amplification cavity in the outer cavity chamber through a pre-designed passage of the centrifugal microfluidic chip by centrifugal force. The temperature uniformity of the outer chamber is mainly completed by a stirring module, and the stirring module drives a stirring impeller to rotate at a high speed by a motor so as to ensure that the air in the chamber exchanges heat at a high speed and meets the requirement of temperature uniformity; the outer chamber temperature control is mainly completed by the assistance of the heating module and the cooling module, when the temperature is detected to be lower than a target temperature value (temperature of each stage of high-temperature denaturation, low-temperature annealing and primer extension), the outer chamber temperature control system enables the heating module to work to emit heat, the cooling module does not work to enable the overall temperature of the outer chamber to rise, and when the temperature is detected to be higher than the target value or higher than the target value (temperature of each stage of high-temperature denaturation, low-temperature annealing and primer extension), the outer chamber temperature control system enables the cooling module to work and the temperature cooling module does not work until the overall temperature of the outer chamber reaches the target value. The inner temperature zone and the outer temperature zone are formed into two relatively independent temperature zones by a centrifugal micro-fluidic chip and a special heat insulation structure of the temperature zones, and the temperatures of the two temperature zones can be separately controlled by respective temperature control systems without interference. The two temperature independent temperature zones can realize different temperature reaction systems in the same centrifugal micro-fluidic chip so as to realize the full automation of the nucleic acid analysis device. The two temperature regions with independent temperatures can be designed for different biochemical reactions and different temperature rise rates.

In addition, other embodiments of the present application include a dual temperature zone device for centrifugation and a microfluidic analysis device, which are formed by combining technical features of the above embodiments with each other and can be implemented.

The technical features of the above embodiments can be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the above embodiments are not described, but should be considered as the scope of the present specification as long as there is no contradiction between the combinations of the technical features. The above examples only express several embodiments of the present application, and the description thereof is more specific and detailed, but not construed as limiting the claims. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the concept of the present application, which falls within the scope of protection of the present application. Therefore, the protection scope of the present application shall be subject to the appended claims.

Claims

1. A dual temperature zone device for centrifugation is characterized in that,

the dual-temperature-zone device is provided with an inner temperature zone and an inner temperature zone opening, and the inner temperature zone is communicated with the outside through the inner temperature zone opening; the dual-temperature-zone device is provided with an internal temperature-zone heating assembly in the internal temperature zone;

the dual-temperature-zone device is provided with an outer temperature zone and an outer temperature zone opening, and the outer temperature zone is used for being communicated with the outside through the outer temperature zone opening; the double-temperature-zone device is provided with an outer-temperature-zone heating assembly in the outer temperature zone;

a heat-insulation isolation structure is arranged between the inner temperature zone and the outer temperature zone, the heat-insulation isolation structure is provided with a groove so that one part of the structure to be centrifuged is accommodated in the inner temperature zone and the other part of the structure to be centrifuged is accommodated in the outer temperature zone, and the heat-insulation isolation structure is used for isolating the air of the inner temperature zone and the air of the outer temperature zone and preventing the heat-insulation isolation structure from blocking the structure to be centrifuged;

the outer temperature zone is provided with an outer temperature zone inlet and a sealing cover structure, and the sealing cover structure is used for sealing or opening the outer temperature zone inlet;

the dual-temperature-zone device also comprises a centrifugal cover structure and a heating structure;

the heating structure is rotationally arranged on the centrifugal cover structure, and the centrifugal cover structure is used for accommodating the structure to be centrifuged;

the heating structure is provided with the inner temperature zone opening and the outer temperature zone opening, and the inner temperature zone heating assembly and the outer temperature zone heating assembly are respectively fixed on the heating structure and face the centrifugal cover structure;

the centrifugal cover structure is provided with the outer temperature zone inlet and the sealing cover structure;

the first isolation part of the heat-insulation isolation structure is fixed on the heating structure, the second isolation part of the heat-insulation isolation structure is fixed on the centrifugal cover structure, and the first isolation part and the second isolation part are matched to form the slot;

the side part of the first isolation part facing the rotation center, the side part of the second isolation part facing the rotation center, the heating structure and the centrifugal cover structure jointly enclose to form the inner temperature zone;

the side part of the first isolation part, the side part of the second isolation part, the heating structure and the centrifugal cover structure are enclosed together to form the outer temperature zone.

2. The dual temperature zone device according to claim 1, further comprising a fixing bracket, wherein the fixing bracket separates the inner temperature zone into an inner heating zone and an inner chamber which are communicated with each other, and the fixing bracket further separates the outer temperature zone into an outer heating zone and an outer chamber which are communicated with each other;

the inner heating zone is used for accommodating the inner temperature zone heating assembly, and the inner chamber is used for accommodating a part of the structure to be centrifuged;

the outer heating zone is used for accommodating the outer temperature zone heating assembly, and the outer chamber is used for accommodating the rest part of the structure to be centrifuged; alternatively, the first and second electrodes may be,

the dual-temperature-zone device also comprises an air duct cylinder, wherein the air duct cylinder is provided with an air duct cylinder cavity and an air duct cylinder inlet, and the air duct cylinder cavity is communicated with the outside through the air duct cylinder inlet; the air duct cylinder cavity is communicated with the outer temperature area through the outer temperature area inlet, and the sealing cover structure is used for sealing or opening the air duct cylinder inlet.

3. The dual-temperature-zone device according to claim 2, further comprising a cold air output device, wherein an output end of the cold air output device is communicated with the air duct inlet through the cover structure, or an output end of the cold air output device is communicated with the outer-temperature-zone inlet through the cover structure.

4. The dual temperature zone device according to claim 1, wherein the dual temperature zone device is provided with an inner temperature control system and an outer temperature control system;

the inner temperature control system is provided with the inner temperature zone and an inner temperature zone opening, and the inner temperature control system is provided with the inner temperature zone heating assembly in the inner temperature zone;

the outer temperature control system is provided with the outer temperature zone and an outer temperature zone opening, and an outer temperature zone heating assembly is arranged in the outer temperature zone of the outer temperature control system;

the inner temperature control system and the outer temperature control system are arranged in a matched mode, and the heat insulation isolation structure is arranged between the inner temperature control system and the outer temperature control system.

5. The dual temperature zone device according to claim 1,

the heating structure is provided with a cover body, a first wall part of the outer temperature zone and a fixed bracket;

the cover body is provided with the inner temperature zone opening and the outer temperature zone opening; the first isolation part is fixed on the cover body and penetrates through the fixed support, the inner temperature zone heating assembly is positioned on one side of the first isolation part, which faces the rotation center, and the outer temperature zone heating assembly is positioned on one side of the first isolation part, which is far away from the rotation center;

the centrifugal cover structure is provided with a cover body, a supporting piece and a second wall part of the outer temperature zone; the cover body is rotatably arranged on the supporting piece, and the supporting piece is fixed on the cover body;

the cover body is provided with the outer temperature area inlet and the cover body is provided with the sealing cover structure; said second separator portion being secured to said centrifuge shield structure;

the side part of the first isolation part facing the rotation center, the cover body and the fixed support jointly enclose an inner heating area of the inner temperature area, and the inner temperature area heating assemblies are all located in the inner heating area;

the side part of the first isolation part, which is far away from the rotation center, the cover body, the first wall part of the outer temperature zone and the fixed support jointly enclose an outer heating zone of the outer temperature zone, and the outer temperature zone heating components are all positioned in the outer heating zone;

the side part of the first isolation part facing the rotation center, the side part of the second isolation part facing the rotation center, the fixed support and the cover body jointly enclose an inner chamber of the inner temperature zone, and the inner chamber is used for accommodating a part of a structure to be centrifuged;

the side part of the first isolation part, the side part of the second isolation part, the fixing support and the cover body enclose an outer chamber of the outer temperature zone, and the outer chamber is used for accommodating the rest part of the structure to be centrifuged.

6. The dual temperature zone device according to claim 5, wherein the internal temperature zone heating element is fixed on the cover or the fixing bracket; and/or the outer temperature zone heating assembly is fixed on the cover body or the fixed support.

7. The dual temperature zone device of claim 1, wherein the internal temperature zone opening is a one-way vent; and/or the opening of the outer temperature area is a one-way ventilation opening.

8. The dual-temperature-zone device according to claim 1, further comprising an inner-chamber temperature detection component connected to the inner-temperature-zone heating component, the inner-chamber temperature detection component being configured to detect an inner-chamber temperature of the inner temperature zone and control the inner-temperature-zone heating component according to the inner-chamber temperature; and/or the dual-temperature-zone device further comprises an outer-cavity temperature detection assembly, the outer-cavity temperature detection assembly is connected with the outer-temperature-zone heating assembly, and the outer-cavity temperature detection assembly is used for detecting the outer-cavity temperature of the outer temperature zone and controlling the outer-temperature-zone heating assembly according to the outer-cavity temperature.

9. The dual-temperature-zone device according to claim 8, wherein the inner-chamber temperature detecting component is used for detecting the inner-chamber temperature of the inner chamber of the inner temperature zone and controlling the inner-temperature-zone heating component according to the inner-chamber temperature; and/or the outer cavity temperature detection assembly is used for detecting the outer cavity temperature of the outer cavity of the outer temperature zone and controlling the outer temperature zone heating assembly according to the outer cavity temperature.

10. The dual temperature zone device according to any one of claims 1 to 9, wherein the thermal insulation isolation structure is configured to rotate synchronously with the structure to be centrifuged; or the heat-insulating isolation structure and the structure to be centrifuged are arranged in a non-contact manner; or the heat-preservation isolation structure is provided with a sliding part for reducing friction force between the heat-preservation isolation structure and the structure to be centrifuged;

the inner temperature zone heating component comprises an inner temperature zone heating module;

the outer temperature zone heating component comprises an outer temperature zone heating module.

11. The dual temperature zone device of claim 10, wherein the internal temperature zone heating assembly further comprises an internal stirring device.

12. The dual temperature zone device of claim 11, wherein the outer temperature zone heating assembly further comprises an outer stirring device.

13. The dual temperature zone device according to claim 12, wherein the dual temperature zone device is further provided with a stirring motor, and the stirring motor is located outside the inner temperature zone and the outer temperature zone; the stirring motor is respectively connected with the inner stirring device and the outer stirring device;

the dual-temperature-zone device is further provided with a rotating shaft for centrifugation and a mounting rack fixed on the rotating shaft, the mounting rack is used for mounting the structure to be centrifuged, and at least part of the mounting rack is located in the internal temperature zone.

14. A microfluidic analytical device comprising a dual temperature zone device according to any of claims 1 to 13.