CN110947024B - Amniotic membrane preparation system and amniotic membrane production system - Google Patents
Amniotic membrane preparation system and amniotic membrane production system Download PDFInfo
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- CN110947024B CN110947024B CN201811126739.1A CN201811126739A CN110947024B CN 110947024 B CN110947024 B CN 110947024B CN 201811126739 A CN201811126739 A CN 201811126739A CN 110947024 B CN110947024 B CN 110947024B
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Classifications
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- A—HUMAN NECESSITIES
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/40—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing ingredients of undetermined constitution or reaction products thereof, e.g. plant or animal extracts
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- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
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- A—HUMAN NECESSITIES
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/005—Ingredients of undetermined constitution or reaction products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/40—Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking
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Abstract
The invention provides an amniotic membrane preparation system and an amniotic membrane production system, and relates to the technical field of amniotic membrane preparation equipment. The amniotic membrane preparation system comprises a storage unit, a cleaning unit, a decellularizing unit, a paving unit, a prefreezing unit, a cutting unit and a liquid control unit, wherein the storage unit, the cleaning unit, the decellularizing unit, the paving unit, the prefreezing unit and the cutting unit are sequentially connected, and the liquid control unit is respectively connected with the cleaning unit, the paving unit and the decellularizing unit. The system can realize continuous amniotic membrane preparation, and each step is automatic, compared with manual operation, the system has the advantages of small error, high preparation efficiency, time and labor saving, low labor cost, high degree of mechanization and strong continuity, can be used for preparing the amniotic membrane in a large scale, and meets the market demand.
Description
Technical Field
The invention relates to the technical field of amniotic membrane preparation equipment, in particular to an amniotic membrane preparation system and an amniotic membrane production system.
Background
The amniotic membrane is used as a natural polymer biological material, contains various components such as collagen, glycoprotein, proteoglycan, integrins, lamellar bodies and the like, expresses various growth factors and related proteins, can provide abundant nutrition components for cell proliferation and differentiation, is beneficial to the growth and propagation of cells, contains various growth factors including basic fibroblast growth factors, platelet growth factors and vascular endothelial growth factors, transforming growth factor beta, hepatocyte growth factors and the like, plays an important role in promoting tissue repair metaphase, can reduce inflammatory reaction, reduce scar tissue formation, and promote the structural repair and functional repair of normal soft tissues. Currently, amniotic membrane is widely used in surgery, wherein the amniotic membrane comprises 1, eye surface diseases such as cornea repair, congenital glaucoma, corneal ulcer and the like, 2, tympanic membrane injury repair, 3, hernia repair, 4, adhesion prevention after surgery, 5, surface coverage after burn, biological dressing and the like.
The existing amniotic membrane preparation methods are various, have no uniform standard, the quality of the prepared amniotic membrane is also nonuniform, most of the amniotic membrane stays in an experimental preparation stage, no large-scale production line is formed, and the amniotic membrane preparation method mainly depends on manual operation, so that the amniotic membrane preparation efficiency is low, the amniotic membrane cost is high, and the market demand cannot be met at all.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention aims to provide an amniotic membrane preparation system which is convenient to operate and capable of automatically preparing amniotic membrane and improving the preparation efficiency.
The invention further aims to provide an amniotic membrane production system which is simple in structure, convenient to operate and high in efficiency and is used for automatically preparing the amniotic membrane.
Embodiments of the present invention are implemented as follows:
the amniotic membrane preparation system comprises a storage unit, a cleaning unit, a decellularizing unit, a paving unit, a prefreezing unit, a cutting unit and a liquid control unit, wherein the storage unit, the cleaning unit, the decellularizing unit, the paving unit, the prefreezing unit and the cutting unit are sequentially connected, and the liquid control unit is respectively connected with the cleaning unit, the paving unit and the decellularizing unit.
Further, in a preferred embodiment of the present invention, the cleaning unit includes at least one cleaning device, the cleaning device includes a base, a stirring tank and a liquid storage tank, the stirring tank is disposed on the base, a first control device and a heating device are disposed in the base, the stirring tank and the heating stirring tank are used for controlling, the liquid storage tank is connected with the stirring tank through a liquid inlet pipe, and a liquid outlet is disposed at the bottom of the liquid storage tank.
Further, in the preferred embodiment of the invention, the stirring tank is internally provided with the net bag, the bottom of the net bag is of a hemispherical structure, and the opening of the net bag is sleeved on the opening of the stirring tank through the ferrule.
Further, in a preferred embodiment of the present invention, the decellularizing unit includes a thermostatic control box, a tray and a spraying device, the spraying device is disposed on a top wall inside the thermostatic control box, and the tray is disposed on a bottom wall inside the thermostatic control box.
Further, in a preferred embodiment of the present invention, the paving unit includes a water tank and a bonding table, the bonding table is disposed in the water tank, the bonding table is movably connected with the water tank, and the bonding table can be lifted relative to the water tank.
Further, in the preferred embodiment of the present invention, the peripheral side wall of the water tank is provided with a plurality of water outlets.
Further, in a preferred embodiment of the present invention, the prefreezing unit includes a freeze-drying device and a freeze-drying box, the freeze-drying device includes a fixing frame, a freeze-drying column and a sleeve, the freeze-drying column is rotationally connected with the fixing frame, the freeze-drying column includes a column body, a cavity is arranged in the column body, the sleeve is sleeved on the freeze-drying column, and a gap is left between the sleeve and the freeze-drying column.
Further, in a preferred embodiment of the present invention, the cutting unit includes a cutting table, a cutting knife, and a second control device, where the second control device is electrically connected to the cutting knife and is used to control the operation of the cutting knife.
Further, in a preferred embodiment of the present invention, the liquid control unit comprises a liquid storage tank, a liquid delivery power device, a flow control valve, and a third control device electrically connected to the liquid delivery power device and the flow control valve for controlling the liquid delivery power device and the flow control valve.
An amnion production system comprises an alarm system and the amnion preparation system, wherein the alarm system is electrically connected with the amnion preparation system.
The beneficial effects of the embodiment of the invention include:
the amnion preparation system comprises a storage unit, a cleaning unit, a paving unit, a cell removing unit, a prefreezing unit, a cutting unit and a liquid control unit, is a whole set of amnion preparation system, can realize continuous amnion preparation, has small error compared with manual operation, has high preparation efficiency, time and labor saving, low labor cost and high mechanical degree, is high in continuity, can be used for preparing the amnion in a large scale, and meets the market demand.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
Fig. 1 is a schematic structural diagram of an amniotic membrane preparation system according to an embodiment of the present invention;
fig. 2 is a schematic structural diagram of a cleaning unit according to an embodiment of the present invention;
FIG. 3 is a schematic structural view of a paving unit according to an embodiment of the present disclosure;
fig. 4 is a schematic structural diagram of a freeze-drying apparatus according to an embodiment of the present invention;
fig. 5 is a schematic structural diagram of a freeze-drying apparatus according to an embodiment of the present invention;
fig. 6 is a schematic structural diagram of a sleeve according to an embodiment of the present invention.
Icon: 100-amniotic membrane preparation system; 110-a storage unit; 120-a cleaning unit; 121-a base; 122-a stirred tank; 1221-a net bag; 1223-handle; 123-a liquid storage tank; 1231-liquid outlet pipe; 1232-liquid outlet valve; 1233-pump; 1234-inlet valve; 1235-liquid inlet pipe; 130-decellularized unit; 140-paving units; 141-a water tank; 143-a laminating table; 145-a clamping groove; 150-a prefreezing unit; 151-a lyophilization apparatus; 1511-a fixing frame; 1512-lyophilization column; 1514-a sleeve; 1514-scaffold; 1515-a first barrel; 1516-a second cylinder; 153-freeze drying oven; 160-clipping unit; 170-a liquid control unit; 171-a storage tank; 172-a fluid delivery power device; 173-a flow control valve; 174-third control means.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments of the present invention. The systems of the embodiments of the invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.
In the description of the present invention, it should be noted that, directions or positional relationships indicated by terms such as "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., are directions or positional relationships based on those shown in the drawings, or are directions or positional relationships conventionally put in use of the inventive product, are merely for convenience of describing the present invention and simplifying the description, and are not indicative or implying that the apparatus or element to be referred to must have a specific direction, be constructed and operated in a specific direction, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used merely to distinguish between descriptions and should not be construed as indicating or implying relative importance.
In the description of the present invention, it should also be noted that, unless explicitly specified and limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
In the present invention, unless expressly stated or limited otherwise, a first feature "above" or "below" a second feature may include both the first and second features being in direct contact, as well as the first and second features not being in direct contact but being in contact with each other through additional features therebetween. Moreover, a first feature being "above," "over" and "on" a second feature includes the first feature being directly above and obliquely above the second feature, or simply indicating that the first feature is higher in level than the second feature. The first feature being "under", "below" and "beneath" the second feature includes the first feature being directly under and obliquely below the second feature, or simply means that the first feature is less level than the second feature.
Some embodiments of the present invention are described in detail below with reference to the accompanying drawings. In the present invention, all the embodiments, implementations and features of the invention may be combined with each other without contradiction or conflict. In the present invention, conventional equipment, devices, components, etc., are either commercially available or homemade in accordance with the present disclosure. In the present invention, some conventional operations and apparatuses, devices, components are omitted or only briefly described in order to highlight the gist of the present invention.
Example 1
Referring to fig. 1, the embodiment provides an amniotic membrane preparation system 100, which includes a storage unit 110, a cleaning unit 120, a decellularization unit 130, a paving unit 140, a prefreezing unit 150, a cutting unit 160, and a liquid control unit 170, wherein the storage unit 110, the cleaning unit 120, the decellularization unit 130, the paving unit 140, the prefreezing unit 150, and the cutting unit 160 are sequentially connected, and the liquid control unit 170 is respectively connected with the cleaning unit 120, the paving unit 140, and the decellularization unit 130.
The storage unit 110 is used for storing amniotic membrane, and in this embodiment, the storage unit 110 is a storage tank. The storage tank is a general device in the technical field, and the invention is not limited thereto.
Referring to fig. 2, the cleaning unit 120 includes at least one cleaning device, and when a plurality of cleaning devices are included, the cleaning devices can be simultaneously cleaned, thereby saving time and improving efficiency. Specifically, the cleaning device includes a base 121, a stirring tank 122 and a liquid storage tank 123, the stirring tank 122 is disposed on the base 121, and a first control device (not shown) and a heating device (not shown) are disposed in the base 121, and are used for controlling the stirring tank 122 and heating the stirring tank 122. A string bag 1221 is arranged in the stirring tank 122, and an opening of the string bag 1221 is sleeved with an opening of the stirring tank 122 through a ferrule. The net 1221 is provided with a handle 1223 for detaching the net 1221.
Because the amniotic membrane is cleaned for multiple times in the stirring tank 122, in order to avoid damage of the amniotic membrane, the amniotic membrane is not moved in the process of cleaning for multiple times, but the cleaning liquid in the stirring tank 122 is replaced. The liquid storage tank 123 is connected with the agitator tank 122 through the feed liquor pipe 1235, and the bottom of liquid storage tank 123 is equipped with the liquid outlet, and the liquid outlet is connected with drain pipe 1231, and drain pipe 1231 is equipped with out liquid valve 1232. The liquid inlet pipe 1235 is provided with a pump 1233 and a liquid inlet valve 1234, and the first control device is electrically connected with the pump 1233, the liquid outlet valve 1232 and the liquid inlet valve 1234, and is used for controlling the opening and closing of the pump 1233, the liquid outlet valve 1232 and the liquid inlet valve 1234.
During cleaning, the amnion to be cleaned is placed in the string bag 1221, the control device of the base 121 controls the pump 1233 and the liquid inlet valve 1234, so that the cleaning liquid enters the stirring tank 122, the heating device heats the stirring tank 122, and then stirring cleaning is started. After cleaning, the handle 1223 is held, and the net bag 1221 is taken out.
Referring to fig. 3, the paving unit 140 includes a water tank 141 and a bonding table 143, the bonding table 143 is disposed in the water tank 141, the bonding table 143 is movably connected with the water tank 141, and the bonding table 143 can be lifted relative to the water tank 141. The attaching base 143 is provided with a clamping groove 145 for fixing the amniotic lining. In this embodiment, the inner lining may be aluminum foil, nitrocellulose filter paper, PVDF film, or the like. The side walls around the water tank 141 are provided with a plurality of water outlets for realizing the unfolding and spreading of the amniotic membrane through water flow. In this embodiment, the water outlet is disposed on the peripheral side wall of the lower portion of the water tank.
The amnion lining is clamped on the attaching table 143, the amnion is put into a water pool, a plurality of water outlets are opened, and water is injected into the water pool. Along with the injection of water around, the amniotic membrane is attached to the lining on the attaching table 143, so that the amniotic membrane is unfolded and paved. The amniotic membrane is paved through the acting force of water, manual paving is omitted, tools such as manual or scraper are avoided, time and strength are saved, the amniotic membrane is prevented from being damaged, and the completeness and quality of the amniotic membrane are ensured.
The decellularizing unit 130 includes a constant temperature control box (not shown), a tray (not shown) and a spraying device (not shown), the spraying device is disposed on a top wall inside the constant temperature control box, and the tray is disposed on a bottom wall inside the constant temperature control box. The tray is used for placing the amniotic membrane, the spraying device is used for spraying the decellularized liquid to the amniotic membrane on the tray, and the constant temperature control box is used for controlling the temperature and time of the decellularized process of the amniotic membrane.
Referring to fig. 4, 5 and 6, the prefreezing unit 150 includes a freeze-drying device 151 and a freeze-drying box 153, the freeze-drying device 151 includes a fixing frame 1511, a freeze-drying column 1512 and a sleeve 1514, the freeze-drying column 1512 is rotatably connected with the fixing frame 1511, the freeze-drying column 1512 includes a column body, and a cavity is disposed in the column body for accommodating silicone oil. In actual use, the amniotic membrane is spread out over the liner and then the liner is fixed to the surface of the lyophilization column 1512. In order to fix and attach the amniotic lining well, two ends of the column body are respectively provided with a clamping piece for fixing the lining. The structure can ensure the heat transfer to the amniotic membrane, so that the amniotic membrane is heated uniformly, wrinkles and white textures can not appear, and the uniform and attractive appearance of the amniotic membrane is ensured.
The sleeve 1514 is sleeved on the freeze-drying column 1512, and in order to avoid contact between the sleeve 1514 and the amniotic membrane on the surface of the freeze-drying column 1512 and damage the shape of the amniotic membrane, a gap is reserved between the sleeve 1514 and the freeze-drying column 1512. Meanwhile, the sleeve 1514 is provided to prevent cold air in the vacuum freeze-drying chamber 153 from directly blowing onto the surface of the amniotic membrane, thereby affecting the beauty of the amniotic membrane.
As one implementation, the amniotic membrane lyophilization apparatus 151 further comprises a support 1514 for supporting the sleeve 1514 such that a gap is left between the sleeve 1514 and the lyophilization column 1512. Specifically, the two ends of the support 1514 are connected with the two ends of the freeze-drying column 1512, a gap is formed between the support 1514 and the freeze-drying column 1512, and the sleeve 1514 is sleeved on the support 1514 and the freeze-drying column 1512.
Because sleeve 1514 is sleeved on the outer surface of freeze-drying column 1512, sleeve 1514 comprises first cylinder 1515 and second cylinder 1516, and first cylinder 1515 and second cylinder 1516 enclose sleeve 1514 for convenient installation and disassembly of amniotic membrane. I.e., first cylinder 1515 and second cylinder 1516 are semi-circular in structure. The side end of first cylinder 1515 is movably connected with the side end of second cylinder 1516, and the free side end of first cylinder 1515 is selectively moved closer to or farther from the free side end of second cylinder 1516.
In this embodiment, the side end of first cylinder 1515 is hinged to the side end of second cylinder 1516 to facilitate opening and closing of the cylinders, and in other embodiments of the invention, first cylinder 1515 and second cylinder 1516 may be connected in other ways, which are not limited by the invention.
Working principle: before lyophilization, the amniotic membrane to be pre-frozen is spread and attached to the inner liner, then the two ends of the inner liner are placed into the annular clamping grooves 145 to clamp, the inner liner is attached to the surface of the lyophilization column 1512, and then the sleeve 1514 is sleeved on the outer surface of the lyophilization column 1512. Because of the support 1514, the support 1514 can support the sleeve 1514 out of contact with the lyophilization column 1512, leaving a gap.
The cutting unit 160 includes a cutting table (not shown), a cutting knife (not shown), and a second control device (not shown) electrically connected to the cutting knife for controlling the operation of the cutting knife. After the pre-freezing unit 150 freeze-dries the amniotic membrane, the amniotic membrane is transferred to a cutting unit 160, and the amniotic membrane is cut.
The liquid control unit 170 is used to provide the required solvent to the washing unit 120, the paving unit 140, and the decellularization unit 130. It includes a liquid storage tank 171, a liquid delivery power device 172, a flow control valve 173, and a third control device 174, the third control device 174 being electrically connected to the liquid delivery power device 172 and the flow control valve 173 for controlling the liquid delivery power device 172 and the flow control valve 173. In this embodiment, the number of the liquid storage tanks 171 and the flow control valves 173 is three, and the three liquid storage tanks 171 are respectively filled with different liquids to respectively supply the required solvents to the washing unit 120, the spreading unit 140 and the decellularization unit 130.
The first control device, the second control device, the third control device 174, the control valve, the constant temperature control box, the spraying device and the stirring tank 122 in this embodiment are all general devices that are used in the present technology, and the present invention is not limited thereto.
Working principle: the amnion is transferred from the storage unit 110 to the cleaning unit 120, the operations of cleaning, virus inactivation, de-scaling and the like are completed in the cleaning unit 120, the cleaned amnion is transferred to the decellularization unit 130, the decellularization liquid is sprayed for decellularization, the amnion is transferred to the prefreezing unit 150 for drying and freezing, and after the dried amnion is obtained, the dried amnion is transferred to the cutting unit 160 for cutting into required sizes, and the required amnion is obtained.
The invention also provides an amniotic membrane production system (not shown), which comprises an alarm system (not shown) and an amniotic membrane preparation system 100, wherein the alarm system is electrically connected with the amniotic membrane preparation system 100, and when at least one unit in the amniotic membrane preparation system 100 stops working, the alarm system gives an alarm, and a worker performs inspection and repair. The alarm system in this embodiment is a general device in the art, and the connection and the operation manner of the alarm system and the amniotic membrane preparation system 100 are known in the art, and the present invention is not limited thereto.
The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, but various modifications and variations can be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (6)
1. The amniotic membrane preparation system is characterized by comprising a storage unit, a cleaning unit, a decellularizing unit, a paving unit, a prefreezing unit, a cutting unit and a liquid control unit, wherein the storage unit, the cleaning unit, the decellularizing unit, the paving unit, the prefreezing unit and the cutting unit are sequentially connected, and the liquid control unit is respectively connected with the cleaning unit, the paving unit and the decellularizing unit;
the cleaning unit comprises at least one cleaning device, the cleaning device comprises a base, a stirring tank and a liquid storage tank, the stirring tank is arranged on the base, a first control device and a heating device are arranged in the base and used for controlling the stirring tank and heating the stirring tank, the liquid storage tank is connected with the stirring tank through a liquid inlet pipe, and a liquid outlet is formed in the bottom of the liquid storage tank;
the cell removing unit comprises a constant temperature control box, a tray and a spraying device, wherein the spraying device is arranged on the top wall inside the constant temperature control box, and the tray is arranged on the bottom wall inside the constant temperature control box;
the paving unit comprises a water tank and a bonding table, the bonding table is arranged in the water tank and is movably connected with the water tank, and the bonding table can be lifted relative to the water tank;
the pre-freezing unit comprises a freeze-drying device and a freeze-drying box, the freeze-drying device comprises a fixing frame, a freeze-drying column and a sleeve, the freeze-drying column is rotationally connected with the fixing frame and comprises a column body, a cavity is arranged in the column body, the sleeve is sleeved on the freeze-drying column, and a gap is reserved between the sleeve and the freeze-drying column.
2. The amniotic membrane preparation system according to claim 1, wherein a string bag is arranged in the stirring tank, the bottom of the string bag is of a hemispherical structure, and an opening of the string bag is sleeved on the opening of the stirring tank through a ferrule.
3. The amniotic membrane preparation system according to claim 1, wherein the circumferential side wall of the water tank is provided with a plurality of water outlets.
4. The amniotic membrane preparation system according to any one of claims 1-3, wherein the cutting unit includes a cutting table, a cutting blade, and a second control device electrically connected to the cutting blade for controlling operation of the cutting blade.
5. The amniotic membrane preparation system according to any one of claims 1-3, wherein the liquid control unit includes a liquid storage tank, a liquid delivery motive device, a flow control valve, and a third control device electrically connected to the liquid delivery motive device and the flow control valve for controlling the liquid delivery motive device and the flow control valve.
6. An amniotic membrane production system comprising an alarm system and the amniotic membrane preparation system of any one of claims 1 to 5, wherein the alarm system is electrically connected to the amniotic membrane preparation system.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104189950A (en) * | 2014-09-04 | 2014-12-10 | 成都清科生物科技有限公司 | Amniotic biological agent and preparation method thereof |
| CN204882129U (en) * | 2015-06-25 | 2015-12-16 | 成都清科生物科技有限公司 | Take off cell amnion freeze -drying diaphragm cutting device |
| WO2017017474A1 (en) * | 2015-07-30 | 2017-02-02 | Ucl Business Plc | Methods and devices for the production of decellularised tissue scaffolds |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN209347705U (en) * | 2018-09-26 | 2019-09-06 | 成都峻宇生物科技有限公司 | Amnion preparation system and amnion production system |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104189950A (en) * | 2014-09-04 | 2014-12-10 | 成都清科生物科技有限公司 | Amniotic biological agent and preparation method thereof |
| CN204882129U (en) * | 2015-06-25 | 2015-12-16 | 成都清科生物科技有限公司 | Take off cell amnion freeze -drying diaphragm cutting device |
| WO2017017474A1 (en) * | 2015-07-30 | 2017-02-02 | Ucl Business Plc | Methods and devices for the production of decellularised tissue scaffolds |
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