CN110940811A - Detection card for simultaneously and quantitatively detecting two toxins in flour as well as preparation and application thereof - Google Patents

Detection card for simultaneously and quantitatively detecting two toxins in flour as well as preparation and application thereof Download PDF

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CN110940811A
CN110940811A CN201911237059.1A CN201911237059A CN110940811A CN 110940811 A CN110940811 A CN 110940811A CN 201911237059 A CN201911237059 A CN 201911237059A CN 110940811 A CN110940811 A CN 110940811A
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colloidal gold
detection
monoclonal antibody
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杜道林
杜霞
洪霞
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Jiangsu Wise Science and Technology Development Co Ltd
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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    • G01N33/532—Production of labelled immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody

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Abstract

The invention discloses a detection card for simultaneously and quantitatively detecting two toxins in flour, which comprises a sample pad, a colloidal gold film, a nitrocellulose film and a water absorption pad; wherein the colloidal gold membrane is coated with a colloidal gold marker for detecting an antibody, and the nitrocellulose membrane is coated with a detection line T1 for vomitoxin antigen, a detection line T2 for fumonisin antigen and a quality control line C for IgG antibody. Its preparing process and application are also disclosed. The invention can simultaneously realize the rapid detection of fumonisins and vomitoxin in the flour, and the detection method is rapid, simple and convenient, low in cost and high in sensitivity.

Description

Detection card for simultaneously and quantitatively detecting two toxins in flour as well as preparation and application thereof
Technical Field
The invention belongs to the technical field of agricultural product safety detection, and particularly relates to a detection card for simultaneously and quantitatively detecting two toxins in flour, and preparation and application thereof.
Background
Fumonisins (FB) are a mycotoxin, which mainly contaminates foodstuffs and their products, and can produce acute toxic reactions and potentially carcinogenic forms.
Vomitoxin, also known as Deoxynivalenol (DON), belongs to trichothecene compounds, is named because it can cause vomiting of pigs, has certain harm to human bodies, and is a third-level carcinogen.
Fumonisins and vomitoxins threaten the health of human beings and livestock all the time, and the existing detection method comprises a colloidal gold lateral chromatography technology which can qualitatively detect the residue of a single type of mycotoxin and meet the requirement of quick field, but can only qualitatively detect the residue, and has the advantages of low sensitivity, strong subjectivity of visual observation and poor repeatability. Therefore, a detection method which can simultaneously detect residues of various fungoids, has strong repeatability and can quantitatively detect the residues is urgently needed to be developed.
Disclosure of Invention
The application provides a detection card for simultaneously and quantitatively detecting two toxins in flour, can simultaneously and rapidly detect pollution of vomitoxin and fumonisin in flour, and is convenient to operate and simple in method.
The technical scheme is as follows:
the detection card for simultaneously and quantitatively detecting two toxins in flour comprises a test strip;
the test strip comprises: the device comprises a sample pad, a colloidal gold film, a nitrocellulose film, a water absorption pad and a bottom plate;
the colloidal gold film is coated with a detection antibody colloidal gold marker, and a detection antibody in the detection antibody colloidal gold marker comprises a vomitoxin monoclonal antibody and a fumonisin monoclonal antibody;
the IgG antibody on the nitrocellulose membrane is a goat anti-mouse IgG antibody.
Preferably, the vomitoxin antigen is vomitoxin-bovine serum albumin coupled antigen; the fumonisin antigen is fumonisin-bovine serum albumin coupled antigen.
Preferably, the detection card further comprises a detection card shell, and the detection card shell is provided with a sample adding window and a detection result display window;
the sample pad, the colloidal gold film, the nitrocellulose film and the water absorption pad are sequentially adhered to the bottom plate, the sample pad and the colloidal gold film are overlapped in a crossed mode, the colloidal gold film and the nitrocellulose film are overlapped in a crossed mode, the nitrocellulose film and the water absorption pad are overlapped in a crossed mode, the test paper strip is placed in the shell of the detection card, the sample pad is located at the sample adding window, and the nitrocellulose film is located at the detection result display window.
The preparation method of the detection card for simultaneously and quantitatively detecting two toxins in flour comprises the following steps: preparing a colloidal gold film, coating a nitrocellulose film, preparing a sample pad and assembling a detection card;
the preparation method of the colloidal gold film comprises the following steps:
(1) preparing a colloidal gold solution: adding ultrapure water and chloroauric acid into a container treated by aqua regia overnight, magnetically heating and stirring until the solution is boiled, adding trisodium citrate solution, continuously stirring and heating until the color is completely changed into transparent mauve, keeping the solution boiling for 1-5min, stopping heating, stirring at room temperature, cooling, and storing for later use;
(2) pretreatment of detection antibody: respectively dialyzing vomitoxin monoclonal antibody and fumonisin monoclonal antibody to be marked with dialysis solution for 12-48h, filtering with microporous membrane, and diluting for later use;
(3) preparation of detection antibody colloidal gold marker: taking two parts of the colloidal gold solution obtained in the step (1), adjusting the pH value of the solution, then respectively adding the vomitoxin monoclonal antibody and the fumonisin monoclonal antibody diluted in the step (2) for reaction, carrying out sealing treatment after the reaction is finished, centrifuging the solution and discarding supernatant after the sealing is finished, and diluting the precipitate to obtain a vomitoxin monoclonal antibody colloidal gold marker and a fumonisin monoclonal antibody colloidal gold marker;
(4) preparing a colloidal gold film: and (3) uniformly mixing the vomitoxin monoclonal antibody colloidal gold marker and the fumonisin monoclonal antibody colloidal gold marker in the step (3) in equal volume, spraying the mixture on a carrier glass cellulose membrane, and naturally airing or drying.
Preferably, the specific method for preparing the colloidal gold film comprises the following steps:
(1) preparing a colloidal gold solution: adding ultrapure water, and adding chloroauric acid (HAuCl) in a container for treating aqua regia overnight4·3H2O) to prepare 0.005-0.05% chloroauric acid aqueous solution, magnetically heating and stirring until the solution boils for 1-3min, and immediately adding 0.5-2% trisodium citrate (Na)3C6H5O7·2H2O) solution, continuously stirring and heatingWhen the color of the solution is completely changed into transparent purple red, keeping the solution boiling for about 1-3min, stopping heating, cooling, and storing for later use; the prepared colloidal gold solution is evaluated through ultraviolet scanning and a transmission electron microscope;
(2) pretreatment of detection antibody: dialyzing the vomitoxin monoclonal antibody and the fumonisin monoclonal antibody to be marked respectively with 0.005M NaCl solution for 12-24h, removing salt ions, filtering by a microporous filter membrane, and diluting to 0.5-5mg/mL by PB;
(3) preparation of detection antibody colloidal gold marker: taking two parts of the colloidal gold solution in the step (1), and using 0.05-0.5mol/L K2CO3Adjusting the pH value of the colloidal gold solution to 8.0, then respectively adding 1.5-10 mu L of vomitoxin monoclonal antibody and fumonisin monoclonal antibody diluted in the step (2), standing and reacting in a constant temperature cabinet at 25 ℃ for 20-50min, then adding BSA (bovine serum albumin) for sealing, standing and reacting in a constant temperature cabinet at 25 ℃ for 20-50min to obtain a gold-labeled antibody solution; centrifuging the gold-labeled antibody solution at 10000r/min for 10-25min, removing the supernatant, collecting the precipitate formed by the nano-gold particles, and diluting the precipitate with a gold-labeled antibody diluent to a constant volume of 1mL to prepare a vomitoxin monoclonal antibody colloidal gold marker and a fumonisin monoclonal antibody colloidal gold marker;
gold-labeled antibody diluent: taking 5-15g of sucrose, 0.5-2g of BSA (calf serum albumin) and 3-8g of trehalose, and preparing a sample pad treatment solution by using 0.02-0.1mol/L of Tris buffer solution for constant volume.
(4) Preparing a colloidal gold film: uniformly mixing the vomitoxin monoclonal antibody colloidal gold marker and the fumonisin monoclonal antibody colloidal gold marker in the step (3) in equal volume, uniformly spraying the mixture on a carrier glass cellulose membrane by using a membrane scratching instrument at the concentration of 2-10 mu L/cm, and naturally airing at room temperature or drying at 37 ℃ for 1-3h to prepare a colloidal gold membrane;
preferably, the sample pad preparation method comprises: and (3) uniformly coating the sample pad treatment solution on a glass cellulose membrane, and naturally airing at room temperature under the condition that the air humidity is lower than 40%.
Preferably, the sample pad treatment solution is prepared by adding PVP-K30, BSA and NaCl to a constant volume of 0.005-0.05mol/L PBS.
More preferably, the sample pad treatment solution is prepared by adding 2.5-2g of PVP-K30, 0.5-2g of BSA and 0.5-2g of NaCl to 0.005-0.05mol/L of PBS to a constant volume.
Preferably, the coating method of the nitrocellulose membrane is as follows: and respectively spraying the IgG antibody, the vomitoxin antigen and the fumonisin antigen on a nitrocellulose membrane by using a membrane scratching instrument, coating the nitrocellulose membrane into a quality control line C, a detection line T1 and a detection line T2, and drying.
Preferably, the nitrocellulose membrane coating method specifically comprises the following steps:
diluting goat anti-mouse IgG antibody to 0.5-5mg/mL with PB (phosphate buffer), spraying on nitrocellulose membrane with a striping instrument at a concentration of 0.5-5 μ L/cm, and coating to obtain IgG antibody quality control line C;
diluting vomitoxin-bovine serum albumin coupled antigen to 0.5-5mg/mL, spraying on a nitrocellulose membrane with a membrane scratching instrument at a concentration of 0.5-5 muL/cm, and coating to form a vomitoxin detection line;
diluting fumonisin-bovine serum albumin coupled antigen to 0.5-5mg/mL, spraying the antigen on a nitrocellulose membrane by using a membrane scratching instrument at the concentration of 0.5-5 mu L/cm, and coating the antigen to form a fumonisin detection line;
drying for 3-6h at 40-70 ℃ after spraying.
Preferably, the assembly of the test card: sequentially adhering a sample pad, a colloidal gold film, a nitrocellulose film and a water absorption pad on the bottom plate to assemble a test strip, and mounting the test strip in a detection card shell after cutting; the sample pad is positioned at the sample adding window, and the nitrocellulose membrane is positioned at the detection result display window.
Preferably, the bottom plate is a PVC rubber plate.
The detection method of the detection card for simultaneously and quantitatively detecting the two toxins in the flour comprises the following steps:
⑴, pretreating a sample to be detected, namely dissolving the flour sample to be detected, fully stirring and mixing, centrifuging and taking supernatant to obtain sample liquid;
⑵, reacting the sample solution with the test card by adding a proper amount of sample solution into the sample pad of the test card, and incubating in a thermostat immediately after adding the sample solution;
⑶, reading the detection result, namely, placing the detection card into a colloidal gold detection device within 5min after the incubation is finished to detect the reading.
Preferably, the pretreatment of the sample to be detected: weighing 0.5-2g of flour sample to be detected, adding 0.5-20mL of 10% methanol solution (methanol: deionized water is 1:9), fully stirring and mixing (3-10min), and centrifuging at 3000-6000rpm for 5-10min to obtain sample liquid.
Preferably, the sample fluid reacts with the test card: dropping 2-5 drops of sample liquid into the sample adding window, immediately adding the sample into a constant temperature box for incubation at 30-45℃ for 5-10min。
The detection card has high sensitivity and high specificity, and can be used for simultaneously and rapidly detecting whether the flour is polluted by vomitoxin and fumonisin; the detection operation is convenient, simple and quick, and the result is accurate, economic and applicable.
Description of the drawings:
FIG. 1: the structure diagram of the colloidal gold detection card;
FIG. 2: the cross-sectional structure diagram of the test strip in the colloidal gold test card;
FIG. 3: colloidal gold scanning electron microscope image.
Detailed Description
As shown in attached figures 1 and 2, the colloidal gold test card for simultaneously testing vomitoxin and fumonisin in flour comprises a test strip 6 and a test card shell 7.
The test strip 6 comprises a sample pad 8, a colloidal gold membrane 9, a nitrocellulose membrane 11, a water absorption pad 12 and a bottom plate 10.
The sample pad 8, the colloidal gold film 9, the nitrocellulose membrane 11 and the absorbent pad 12 are sequentially fixed on the bottom plate 10, the sample pad 8 and the colloidal gold film 9 are overlapped in a crossing manner, the colloidal gold film 9 and the nitrocellulose membrane 11 are overlapped in a crossing manner, and the nitrocellulose membrane 11 and the absorbent pad 12 are overlapped in a crossing manner. The base plate 10 is a PVC rubber plate.
The detection card shell 7 is provided with a sample adding window 1 and a detection result display window 5. The test strip 6 is arranged in the shell 7 of the test card, the sample pad 8 is positioned at the position of the sample adding window 1, and the nitrocellulose membrane 11 is positioned at the position of the test result display window 5.
The colloidal gold film 9 is a glass cellulose film and is coated with a vomitoxin monoclonal antibody colloidal gold marker and a fumonisin monoclonal antibody colloidal gold marker; the nitrocellulose membrane 11 is provided with a detection line T1, a detection line T2 and a quality control line C, the detection lines T1 and T2 are arranged in sequence from the end 1 of the sample adding window, and are respectively coated with vomitoxin-bovine serum albumin coupling antigen and fumonisin-bovine serum albumin coupling antigen, and the quality control line C is coated with goat anti-mouse IgG antibody.
Preparation of the detection card:
(1) preparing a colloidal gold solution: taking 1 flask of 150mL, treating the flask with aqua regia overnight, adding 100mL of ultrapure water, and adding 1% chloroauric acid (HAuCl)4·3H2O)1mL, preparing 100mL of 0.01% chloroauric acid aqueous solution, magnetically heating and stirring until the solution boils for 2min, and immediately adding 0.8mL of 2% trisodium citrate (Na)3C6H5O7·2H2O) continuing stirring and heating the solution, keeping the solution boiling for about 2min when the color of the solution is completely changed into transparent purple red, stopping heating, stirring and cooling at room temperature, storing at 4 ℃ for later use, and evaluating the prepared colloidal gold solution by ultraviolet scanning and a transmission electron microscope; as shown in FIG. 3, the size of the colloidal gold particles is 18-25 nm;
(2) pretreatment of detection antibody: dialyzing vomitoxin monoclonal antibody and fumonisin monoclonal antibody to be marked with 0.005M NaCl solution for 24h, removing salt ions, filtering with 0.22 μ M microporous membrane, and diluting with 0.01mol/LPB to 1 mg/mL;
(3) preparation of detection antibody colloidal gold marker: taking 1mL of the colloidal gold solution obtained in the step (1) in two parts, and using 0.2mol/LK2CO3Adjusting the pH value of the colloidal gold solution to 8.0, then respectively adding 5 mu L of vomitoxin monoclonal antibody (1mg/mL) and fumonisin monoclonal antibody (1mg/mL) diluted in the step (2), standing and reacting for 30min in a constant temperature box at 25 ℃, adding 100 mu L of 19% BSA (bovine serum albumin) for sealing, and standing and reacting for 30min in the constant temperature box at 25 ℃; after the reaction is finished, centrifuging the solution at 8000r/min for 15min, removing supernatant, collecting precipitate formed by nano-gold particles, and diluting the precipitate with a gold-labeled antibody diluent to a constant volume of 1mL to prepare a vomitoxin monoclonal antibody colloidal gold marker and a fumonisin monoclonal antibody colloidal gold marker;
preferably, the gold-labeled antibody dilution: a sample pad treatment solution was prepared by taking 10g of sucrose, 1g of BSA (calf serum albumin) and 5g of trehalose and diluting to 100mL with 0.05mol/L of the buffer solution.
(4) Preparing a colloidal gold film: uniformly mixing the vomitoxin monoclonal antibody colloidal gold marker and the fumonisin monoclonal antibody colloidal gold marker in the step (3) in equal volume, uniformly spraying the mixture on a carrier glass cellulose membrane by using a membrane scratching instrument at the concentration of 5 mu L/cm, and naturally airing at room temperature or drying at 37 ℃ for 3 hours to prepare a colloidal gold membrane;
(5) coating of nitrocellulose membrane: diluting goat anti-mouse IgG antibody to 1mg/mL with 0.01mol/LPB (phosphate buffer), spraying on nitrocellulose membrane at a concentration of 1 μ L/cm with a membrane drawing instrument, and coating to obtain quality control line C; diluting vomitoxin coupling antigen to 1mg/mL, spraying on a nitrocellulose membrane with a membrane scratching instrument at the concentration of 1 muL/cm, and coating to form a vomitoxin detection line T1; diluting the fumonisin coupled antigen to 1mg/mL, spraying the fumonisin coupled antigen on a nitrocellulose membrane by using a membrane scratching instrument at the concentration of 1 mu L/cm, and coating the fumonisin coupled antigen to a test line T2; drying for 4h at 60 ℃ after spraying; the detection line T1, the detection line T2 and the quality control line C on the nitrocellulose membrane are sequentially arranged from the sample pad to the absorbent pad (as shown in figure 1).
(6) Preparation of sample pad: taking 1g of PVP-K30 (polyvinylpyrrolidone-K30), 1g of BSA (calf serum albumin) and 1g of NaCl (sodium chloride), and making a sample pad treatment solution by using 0.01mol/LPBS (phosphate buffer solution) to fix the volume to 100 mL; uniformly coating the sample pad treatment solution on a glass cellulose membrane, and naturally airing at room temperature under the condition that the air humidity is lower than 40%;
(7) assembling the detection card: sequentially adhering a sample pad, a colloidal gold film, a nitrocellulose membrane and a water absorption pad on a bottom plate to assemble a test strip (shown in figure 2), cutting the test strip into 3.2mm multiplied by 60mm, and installing the test strip in a shell of a detection card to ensure that the sample pad is positioned at a sample adding window and the nitrocellulose membrane is positioned at a detection result display window.
The detection method comprises the following steps:
weighing 1g of flour sample to be detected, adding 10mL of 10% methanol solution (methanol: deionized water is 1:9), fully stirring and mixing for 5min, centrifuging at 4000rpm for 5min, sucking supernatant, dropwise adding 3 drops of the supernatant into a sample adding window, immediately placing the sample in a constant temperature box for incubation at 37 ℃ for 10min, and placing a detection card in a colloidal gold food safety analyzer (HG-1721) within 5min after the incubation is finished to detect and read, thus completing sample detection.
Example 1
Flour was mixed with different masses of vomitoxin and fumonisin, configured into 6 toxin flour and blank controls and numbered: the flour numbered 1-3 contains vomitoxin with contents of 50, 100 and 200 mug/kg in sequence, 5-7 contains fumonisin with contents of 100, 200 and 500 mug/kg in sequence, and numbers 4 and 8 are blank references. 7 samples were tested in the above test method using two toxin test cards, 3 replicates, and the results are shown in Table 1.
The results show that the recovery rate of the vomitoxin in the flour sample by the detection cards of the two toxins is 90.4-113.4%, and the coefficient of variation is 4.25% -9.20%; the recovery rate range of fumonisins in the flour sample is 93.4-111.7%, and the coefficient of variation is 5.69-8.79%, and in conclusion, the recovery rate variation range and the coefficient of variation of the two toxin detection cards are within 25% and within 15%, which indicates that the detection card has good accuracy and precision.
TABLE 1 test for the recovery of emetic and fumonisins by addition to flour samples
Figure BDA0002305161810000091
Example 2
Randomly selecting 10 kinds of market flour, detecting 10 flour samples by using two toxin detection cards according to the detection method, setting blank control, repeating each part for 3 times, and comparing the result with the HPLC detection result (shown in table 2). The result shows that the coincidence rate of the two is 100%, and the detection card has an advantage in sensitivity under the condition that the vomitoxin content in flour is lower than 90 (mu g/kg) and the fumonisin content is lower than 20 (mu g/kg).
TABLE 2 comparison of the results of flour determination and instrument determination using two types of quantitative toxin test cards
Figure BDA0002305161810000092
Figure BDA0002305161810000101

Claims (3)

1. The detection card for simultaneously and quantitatively detecting two toxins in flour is characterized by comprising a test strip;
the test strip comprises: the device comprises a sample pad, a colloidal gold film, a nitrocellulose film, a water absorption pad and a bottom plate;
the colloidal gold film is coated with a detection antibody colloidal gold marker, and a detection antibody in the detection antibody colloidal gold marker comprises a vomitoxin monoclonal antibody and a fumonisin monoclonal antibody;
the nitrocellulose membrane is respectively provided with a detection line and a quality control line; the detection lines comprise a detection line coated with vomitoxin antigen and a detection line coated with fumonisin antigen; the quality control line is coated with IgG antibody; the IgG antibody is a goat anti-mouse IgG antibody.
2. The preparation method of the detection card for simultaneously and quantitatively detecting two toxins in flour is characterized by comprising the following steps: preparing a colloidal gold film, coating a nitrocellulose film, preparing a sample pad and assembling a detection card;
the preparation method of the colloidal gold film comprises the following steps:
(1) preparing a colloidal gold solution;
(2) pretreatment of detection antibody: respectively dialyzing vomitoxin monoclonal antibody and fumonisin monoclonal antibody to be marked with dialysis solution for 12-48h, filtering with microporous membrane, and diluting for later use;
(3) preparation of detection antibody colloidal gold marker: taking two parts of the colloidal gold solution prepared in the step (1), adjusting the pH value of the solution, then respectively adding the vomitoxin monoclonal antibody and the fumonisin monoclonal antibody diluted in the step (2) for reaction, carrying out sealing treatment after the reaction is finished, centrifuging the solution, discarding supernatant after the sealing is finished, and diluting the precipitate to obtain a vomitoxin monoclonal antibody colloidal gold marker and a fumonisin monoclonal antibody colloidal gold marker;
(4) preparing a colloidal gold film: and (3) uniformly mixing the vomitoxin monoclonal antibody colloidal gold marker and the fumonisin monoclonal antibody colloidal gold marker in the step (3) in equal volume, spraying the mixture on a carrier glass cellulose membrane, and naturally airing or drying.
3. The detection method for simultaneously and quantitatively detecting the two toxins in the flour is characterized by comprising the following steps of:
⑴, pretreating a sample to be detected, namely dissolving the flour sample to be detected, fully stirring and mixing, centrifuging and taking supernatant to obtain sample liquid;
⑵, reacting the sample solution with the test card, adding a proper amount of the sample solution to the sample pad of the test card of claim 1 or 2, and incubating in a thermostat immediately after the sample is added;
⑶, reading the detection result, namely, placing the detection card into a colloidal gold detection device within 5min after the incubation is finished to detect the reading.
CN201911237059.1A 2019-12-05 2019-12-05 Detection card for simultaneously and quantitatively detecting two toxins in flour as well as preparation and application thereof Pending CN110940811A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1410771A (en) * 2002-01-17 2003-04-16 大连普瑞康生物技术有限公司 Aflatoxin fast detecting apparatus and its making method
CN101281195A (en) * 2008-05-19 2008-10-08 中国计量学院 Colloidal gold immune chromatography test paper for detecting biotoxin and detecting method thereof
CN204314301U (en) * 2014-12-26 2015-05-06 北京康源泰博生物科技有限公司 The immuno-chromatographic test paper strip of a kind of synchronous detection vomitoxin and fumonisin
WO2017211214A1 (en) * 2016-06-07 2017-12-14 江南大学 Preparation method of salmonella-detecting colloidal gold test strip for food based on salmonella core polysaccharide monoclonal antibody

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1410771A (en) * 2002-01-17 2003-04-16 大连普瑞康生物技术有限公司 Aflatoxin fast detecting apparatus and its making method
CN101281195A (en) * 2008-05-19 2008-10-08 中国计量学院 Colloidal gold immune chromatography test paper for detecting biotoxin and detecting method thereof
CN204314301U (en) * 2014-12-26 2015-05-06 北京康源泰博生物科技有限公司 The immuno-chromatographic test paper strip of a kind of synchronous detection vomitoxin and fumonisin
WO2017211214A1 (en) * 2016-06-07 2017-12-14 江南大学 Preparation method of salmonella-detecting colloidal gold test strip for food based on salmonella core polysaccharide monoclonal antibody

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