CN110935060A

CN110935060A - Orthopedic implant and preparation method thereof

Info

Publication number: CN110935060A
Application number: CN201911364855.1A
Authority: CN
Inventors: 孙中业; 虞连奎
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-12-26
Filing date: 2019-12-26
Publication date: 2020-03-31

Abstract

The invention discloses an orthopedic implant and a preparation method thereof, firstly, a hydrothermal method is used for preparing rod-shaped TiO on a porous orthopedic implant₂To do so byIncreasing the surface area of the porous orthopedic implant, modifying the immunosuppressant cyclosporin to bind the cyclosporin to the TiO rods of the primary orthopedic implant component₂On the surface, TiO₂The rod-shaped structure greatly increases the drug-loading rate of the cyclosporine; when the orthopedic implant is implanted into a human body, the cyclosporine can be slowly released to inhibit the proliferation and functions of macrophages such as T cells, B cells and the like related to immune response, so that the immune response of an antibody and the rejection of human muscles to the orthopedic implant are reduced, and the pain of a patient is reduced; and further modifying the orthopedic implant by polydopamine, loading nano silver ions and controlling the release speed of the nano silver ions, so that the orthopedic implant material with biocompatibility and long-term antibacterial performance is prepared, and the application of the orthopedic implant material in clinic and industrialization is realized.

Description

Orthopedic implant and preparation method thereof

Technical Field

The invention relates to the technical field of preparation of orthopedic medical instruments, in particular to an orthopedic implant and a preparation method thereof.

Background

The orthopedic implant refers to a device which is introduced into the human body to replace the tissue or remain in the operation position in the body by an invasive method; medical devices that are partially introduced into the body by invasive means and remain in the operative position for at least 30 days may also be considered implants. Mainly bone engaging implants and bone and joint replacements, etc.

The orthopedic implant belongs to medical consumables with high added value, has various product types, is widely applied to various orthopedic surgeries and has large market demand. At present, most orthopedic implant products depend on foreign import, so that the cost is high. Orthopedic implant products have complicated three-dimensional structure more, and domestic enterprise adopts traditional machining technology production usually, and the process is loaded down with trivial details, and raw and other materials utilization ratio is low, and manufacturing cost is high, is difficult to automatic batch production, and this will lead to traditional processing technology can't satisfy the demand that increases rapidly.

Patent document CN201410015648.6 discloses a method for preparing an orthopedic implant, which comprises mixing metal powder or ceramic powder with a binder, heating, mixing, cooling, and granulating; injecting the feed into a mold at a proper injection temperature, injection pressure and injection speed, and maintaining the pressure and cooling to obtain a green body; sequentially carrying out solvent degreasing and thermal degreasing on the green body to obtain a degreased body; and placing the degreased blank in a sintering furnace, heating to 800-1100 ℃ under vacuum, then heating to 1300-1600 ℃ under argon atmosphere, and sintering for 90-300 min to obtain the orthopedic implant.

Although the preparation method of the invention can realize the one-step molding and mass production of the orthopedic implant, has high utilization rate of raw materials and low cost, the method only improves the preparation of the orthopedic implant from a machining technology to a metal injection one-step molding technology, but due to the existence of the immune system of a human body, the immune system of the human body has natural rejection reaction to foreign matters entering the human body, the rejection reaction is mainly manifested as pain, the wound is difficult to heal and other symptoms, and the pain of a patient can be increased. Therefore, how to reduce or eliminate rejection of the human immune system to the orthopedic implant is a problem which needs to be solved urgently at present.

Disclosure of Invention

The invention aims to provide an orthopedic implant and a preparation method thereof aiming at the problems in the prior art.

In order to achieve the purpose, the invention adopts the technical scheme that:

a method of making an orthopedic implant, comprising the steps of:

s1, preparing the porous orthopedic implant by using a 3D printing technology;

s2, mixing TiO₂Dissolving in KOH solution, placing in a hydrothermal reaction kettle after ultrasonic dispersion is uniform, placing the porous orthopedic implant prepared in the step S1 in the hydrothermal reaction kettle, carrying out hydrothermal reaction, cooling to room temperature after the reaction is finished, taking out, washing to be neutral by deionized water, and then carrying out high-temperature sintering treatment;

s3, preparing a binding solution: dissolving cyclosporine in deionized water, and then adding HBr and ZnBr₂Heating and dissolving to obtain a binding solution;

s4, adding the bonding liquid obtained in the step S3 into a container, heating the bonding liquid to 60-70 ℃, soaking iridium oxide into the bonding liquid to serve as an anode, soaking the porous orthopedic implant primary piece prepared in the step S2 into the bonding liquid to serve as a cathode, and continuously electrifying direct current for 2-3 hours to obtain an orthopedic implant finished product;

s5, immersing the finished product of the orthopedic implant prepared in the step S4 into a dopamine hydrochloride solution, and oscillating for 20-24 hours at a constant temperature of 30-45 ℃;

and S6, under the condition of keeping out of the sun, immersing the orthopedic implant processed in the step S5 into a silver nitrate solution, taking out the orthopedic implant after immersion, washing, drying, and irradiating by ultraviolet light to obtain the orthopedic implant.

As a further limitation of the above technical solution, in step S1, the porous orthopedic implant is selected from any one of a porous titanium implant, a porous titanium alloy implant, a porous magnesium implant and a porous magnesium alloy implant, and the pore diameter is 500-700 μm.

As a further limitation of the above technical solution, in step S2, the hydrothermal reaction is carried out at 180 ℃ for 12 hours; the high-temperature sintering treatment temperature is 500 ℃ for 3 h.

As a further limitation of the above technical solution, in step S3, the method for preparing the binding solution comprises: according to the mass portion, 10 portions of cyclosporine are taken and dissolved in 120 portions of 100-one deionized water, and then 1-2 portions of HBr and 0.5-0.8 portion of ZnBr are added₂Heating to 60-70 deg.C, and reacting for 60-80min to obtain binding solution.

As a further limitation of the above technical solution, in step S4, ultrasonic wave is used for intermittent stirring during the bonding process, wherein the ultrasonic stirring power is 800-1000W, and the frequency is 20-30 KHz.

As a further limitation of the above technical solution, in step S4, the distance between the cathode and the anode is controlled to be 3-5cm, and the current is controlled to be 0.2-0.6A.

As a further limitation of the above technical solution, in step S5, the concentration of dopamine hydrochloride is 2 to 5 mg/mL.

As a further limitation of the technical scheme, in the step S6, the concentration of the silver nitrate solution is 0.02-0.5 mol/L.

Another object of the present invention is to provide an orthopedic implant made according to the above method.

Compared with the prior art, the invention has the beneficial effects that:

(1) firstly, preparing rod-shaped TiO on a porous orthopedic implant by a hydrothermal method₂To increase the specific surface area of the porous orthopedic implant, and then the immunosuppressant cyclosporin is modified to bind the cyclosporin to the rod-shaped TiO of the primary orthopedic implant component₂On the surface, TiO₂The rod-shaped structure greatly increases the ringDrug loading of the sporimycin; when the orthopedic implant is implanted into a human body, the cyclosporine can be slowly released to inhibit the proliferation and functions of macrophages such as T cells, B cells and the like related to immune reaction, so that the antibody immune reaction is reduced, the rejection reaction of human muscles to the orthopedic implant is reduced, and the pain of a patient is reduced; and further modifying the nano-silver particles by using polydopamine, loading the nano-silver particles and controlling the release speed of the nano-silver particles, so that the nano-silver particles have biocompatibility and long-term antibacterial performance.

(2) The porous orthopedic implant is prepared by the 3D printing technology, the production efficiency is high, and batch production can be carried out; the density of the prepared porous orthopedic implant is close to that of human skeleton, and the implant has good biomechanical compatibility, can promote the proliferation and differentiation of osteocytes and promote the growth and healing of the skeleton.

(3) The porous orthopedic implant prepared by the preparation method provided by the invention has low cytotoxicity and good bone promoting effect.

Drawings

Fig. 1 (a) and (b) show the sem characterization results of the orthopedic implants prepared in example 1 after the processing in steps S2 and S6, respectively.

FIG. 2 is a graph showing Ag in PBS for the orthopedic implant samples prepared in examples 1-3⁺Release profile.

FIG. 3 shows the results of the antibacterial ratio (Rp) of planktonic bacteria around the samples of orthopedic implants obtained in examples 1 to 3.

FIG. 4 shows the results of antibacterial ratio (Ra) of bacteria adhered to the surface of the samples of orthopedic implants prepared in examples 1 to 3.

FIG. 5 shows the results of cell compatibility experiments after culturing cells on the surface of the orthopedic implant samples prepared in examples 1-3 for 1d and 3 d.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more clearly apparent, the present invention is further described in detail with reference to the following embodiments; it should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention; reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.

In the following embodiment of the present invention, the prepared orthopedic implant is soaked in a PBS buffer solution, and then the concentration of silver in PBS is detected to observe the silver release condition of the sample. The samples were soaked in 6mL PBS for 1d in dark and then removed for re-soaking in 6mL fresh PBS. This process is repeated until the total soaking time is 14 days. The PBS solution collected at several time points is selected to measure the silver content by ICP-MS, and the silver release of the sample is evaluated.

(2) Antibacterial testing of orthopedic implants

a) Quantitative test of bacteriostasis rate

The bacteria used in the bacteriostasis experiment are staphylococcus aureus. 0.5mL of the cell suspension was added to 9mL of BHI medium, and the mixture was shake-cultured at 37 ℃ for 24 hours for activation. The activated bacteria were diluted in a gradient to an OD of 0.1. The number of planktonic bacteria was determined by serial dilution by plate colony counting. To determine the number of adherent bacteria, the non-adherent bacteria were removed by first rinsing the titanium plate with 3 aspirates of PBS (1mL) using a tip, followed by vortexing in a centrifuge tube (containing 2mL PBS) for 30s and vortexing for 15 min. The number of bacteria in the resulting suspension was then determined using the same method as planktonic bacteria. The same samples were used for re-incubation, then thoroughly cleaned and dried and sterilized. Using the above method, the numbers of planktonic bacteria and adherent bacteria were also determined on days 7 and 14. Between two subsequent time points, samples were stored in 3mL PBS, which was refreshed daily. The antibacterial rate of planktonic bacteria in the medium and the antibacterial rate of live bacteria adhered to the surface of the sample were calculated by the following formulas:

antibacterial rate of planktonic bacteria around coating sample

Antibacterial rate of bacteria adhered to surface of coating sample

Wherein: a is the number of viable bacteria in the culture medium surrounding the orthopedic implant sample, B is the number of viable bacteria in the culture medium not cultured with the sample (control); c is the viable count of the surface of the orthopedic implant sample, and D is the viable count of the surface of the porous orthopedic implant prepared by using a 3D printing technology.

(3) In vitro cell testing of orthopedic implants

Cytotoxicity is an important factor limiting the application of antibacterial agents applied to implants. The titanium oxide and silver nanoparticles have good biocompatibility, and in order to verify the biocompatibility of the orthopedic implant prepared by the invention, an MTT (methyl thiazolyl tetrazolium) experiment is carried out. The specific experimental steps are as follows:

the preparation method of the MTT solution comprises the following steps: MTT (1.0g) was weighed out and dissolved in phosphate buffered saline (PBS, 100mL), sterilized and stored in a refrigerator at 4 ℃ in the dark. Preparation of a culture medium solution: 45mL of high-sugar culture solution, 5mL of newborn bovine serum and 500. mu.L of streptavidin solution are uniformly mixed and stored in a refrigerator at 4 ℃ in the dark. The sample in vitro cell compatibility test object adopts 293-A cells, and the specific experimental steps are as follows:

a. placing the sterilized sample into a 24-pore plate;

b. taking 293-A cells with proper growth cycle, removing culture medium, digesting the cells with pancreatin, and diluting with BMEM culture medium to 1x10⁴Single cell suspension/mL, seeded in 24-well plates (set experimental, blank and control), with blank without material, 3 replicates per group. In the presence of 5% CO₂Culturing in a cell culture box at 37 deg.C for 1, 4, and 7 days;

c. after the incubation is finished, adding a certain amount of MTT solution (10 mu L, 10mg/mL) into each well, and continuously culturing at the temperature of 37 ℃ for 4 h;

d. the 24-well plate was removed, the supernatant gently aspirated from each well (care was taken not to aspirate the blue-violet crystals at the bottom of the well), 100. mu.L of DMSO solution was added to each well, and the well bottom crystals were allowed to dissolve thoroughly by shaking on a shaker for 15min at low speed. Using a pipette gun, 100. mu.L of the solution in each well was transferred to a new 96-well plate, and the absorbance OD value at a wavelength of 490nm was measured using a multifunctional microplate reader.

e. Cell viability was measured as follows: .

Over a range of cell numbers, MTT formazan formation was directly proportional to cell number, and absorbance values (OD) were directly reflective of blue-violet crystalline formazan formation. Thus, the larger the OD value, the larger the number of living cells and the stronger the cell activity.

In order to observe the survival of cells more intuitively, we observed the cells after calcein and PI staining by using a fluorescence microscope, and the specific steps are as follows:

a. the sample was sterilized and placed in a 6-well plate, and 2mL of a 10/mL cell culture solution was inoculated onto the surface. Setting experimental group, control group and blank group, wherein the blank group is not added with cells and is added with 5% CO₂Culturing for 24 hours under the condition of 37 ℃;

b. after 24 hours of incubation, the samples were removed from the culture wells. Digesting the cells on the lower sample by pancreatin, and pouring the cells into a new pore plate;

c. the well plate was centrifuged and the medium was discarded, followed by gentle centrifugal rinsing twice with sterile PBS. Adding 1mL of calcein dye and 1mL of pyridine iodide dye into each hole in a dark room, and incubating for 25min at room temperature in a dark place;

d. the incubated sample was discarded from the dye, gently rinsed twice with sterile PBS and finally observed with a fluorescence microscope. Live cells showed green fluorescent spots and dead cells showed red fluorescent spots.

The present invention will be described in further detail below with reference to specific embodiments and with reference to the attached drawings.

Example 1

A method of making an orthopedic implant, comprising the steps of:

s1, preparing the porous orthopedic implant with interconnected pores by using a 3D printing technology;

an electronic beam melting device with the model of EBM S12, which is produced by ARCAM company in Sweden, is used for printing an individualized and adaptive porous Ti-6Al-4V implant, the aperture is 600 mu m, the pillar diameter is 400 mu m, and the implant has a diamond lattice pore structure;

s2, mixing 1g TiO₂Dissolving the porous bone implant in 50mL of 10mol/L KOH solution, placing the solution in a hydrothermal reaction kettle after uniform ultrasonic dispersion, placing the porous bone implant prepared in the step S1 in the hydrothermal reaction kettle, carrying out hydrothermal reaction for 12 hours at 180 ℃, cooling to room temperature after the reaction is finished, taking out the porous bone implant, washing the porous bone implant to be neutral by deionized water, and carrying out high-temperature sintering treatment for 3 hours at 500 ℃;

s3, preparing a binding solution: according to the mass portion, 10 portions of cyclosporine are taken and dissolved in 110 portions of deionized water, and then 1.5 portions of HBr and 0.6 portion of ZnBr are added₂Heating to 65 ℃, and reacting for 70min to obtain a combined solution;

s4, adding the bonding liquid obtained in the step S3 into a container, heating the bonding liquid to 65 ℃, soaking iridium oxide into the bonding liquid to serve as an anode, soaking the porous orthopedic implant primary piece prepared in the step S2 into the bonding liquid to serve as a cathode, and performing intermittent stirring by adopting ultrasonic waves in the bonding process, wherein the ultrasonic stirring power is 900W, the frequency is 25KHz, the distance between the cathode and the anode is controlled to be 4cm, the current is 0.4A, and the direct current is continuously supplied for 2.5 hours to obtain an orthopedic implant finished product;

s5, immersing the orthopedic implant finished product prepared in the step S4 in 3mg/mL dopamine hydrochloride solution, and oscillating for 20 hours at a constant temperature of 40 ℃;

and S6, under the condition of keeping out of the sun, immersing the orthopedic implant processed in the step S5 in 0.5mol/L silver nitrate solution for 1h, taking out after immersion, washing, drying, and irradiating for 1h by using ultraviolet light with the wavelength of 250nm to obtain the orthopedic implant.

FIG. 1 (a) and (b) are the SEM representation results of the orthopedic implants processed in steps S2 and S6 of this example, and it can be seen from the results that the orthopedic implants are coated with a layer of TiO with an average rod diameter of about 100nm and arranged in order after being processed by hydrothermal method₂The nano rods have uniform sizes and do not have the defects of disordered stacking and the like; after further modification of polydopamine, the silver nanoparticles obtained by the impregnation photochemical reduction method have uniform size and are uniformly distributed on TiO₂On the nano-rod, the sample still has a good surface structure after the nano-silver particles are loaded.

Examples 2 to 3

Examples 2-3 provide a method of making an orthopedic implant, differing from example 1 in that: in step S6, the concentration of the silver nitrate solution is changed, and the rest is the same as that in example 1, and is not repeated here, and the specific experimental condition parameters are shown in the following table.

Examples	Silver nitrate solution concentration (mol/L)
		2	0.02
3	0.5

FIG. 2 is a graph showing Ag in PBS for the orthopedic implant samples prepared in examples 1-3⁺And (3) a release diagram, wherein the results show that the release amount of the silver of different samples is in the following sequence: example 3>Example 1>Example 2, and at the beginning of the experiment, the amount of released silver ions was large for each sample, with the sample prepared in example 3 releasing the largest amount, and then the amount of released silver gradually decreased with the increase of the soaking time; and after two weeks, Ag⁺The above results indicate that the orthopedic implant prepared by the method of the present invention has a longer silver ion release period in PBS, thereby contributing to the reduction of Ag⁺Toxicity and rejection reactions caused by the release of the drug to the body; but when the concentration of the silver nitrate solution is too high, Ag⁺The release amount is higher, and the concentration of the silver nitrate solution is 0.1 mol/L.

FIG. 3 shows the results of the antibacterial ratio (Rp) of planktonic bacteria around the orthopedic implant samples prepared in examples 1 to 3, and it can be seen from the graph that the Rp value of the sample decreases with time and the antibacterial ratio (Rp) of planktonic bacteria around the sample with a low silver content decreases at a higher rate. The antibacterial rate (Rp) of planktonic bacteria around the orthopedic implant samples prepared in example 1 and example 3 was about 100% in the first two days; by day 14, the antibacterial ratio (Rp) of planktonic bacteria around the orthopedic implant sample prepared in example 1 was about 83%; the antibacterial rate (Rp) of planktonic bacteria around the orthopedic implant sample prepared in example 2 is about 69%; the antibacterial ratio (Rp) of planktonic bacteria around the orthopedic implant sample prepared in example 3 was about 87%.

FIG. 4 shows the results of the antibacterial rate (Ra) against bacteria adhered to the surface of the samples of the orthopedic implants obtained in examples 1 to 3, and it can be seen that the Ra value of the samples decreases with time, and the decrease rate of the antibacterial rate (Ra) against bacteria adhered to the surface of the samples with a low silver content is faster. The antibacterial rate (Ra) of the bacteria adhered to the surfaces of the orthopedic implant samples prepared in the example 1 and the example 3 is about 100 percent in the first two days; by day 14, the antibacterial rate (Ra) of the bacteria adhered to the surface of the orthopedic implant sample prepared in example 1 was about 79%; the antibacterial rate (Ra) of the bacteria adhered to the surface of the orthopedic implant sample prepared in the example 2 is about 63 percent; the antibacterial rate (Ra) of the bacteria adhered to the surface of the orthopedic implant sample prepared in example 3 was about 82%.

FIG. 5 shows the results of cell compatibility experiments after culturing cells on the surface of the orthopedic implant samples prepared in examples 1-3 for 1d and 3d, and it can be seen from the results in the figure that the orthopedic implant sample containing a proper amount of silver has better biocompatibility, but when the silver loading exceeds 0.1mol/L, the prepared orthopedic implant sample shows higher cytotoxicity. Therefore, the concentration of the silver nitrate solution is preferably 0.02-0.1 mol/L, and the prepared orthopedic implant sample shows good cell proliferation, so that the surface of the orthopedic implant sample loaded with a proper amount of silver can improve the antibacterial performance of the implant and has good biocompatibility.

Examples 4 to 6

Examples 4-6 provide a method of making an orthopedic implant, as compared to example 1, with the following differences: in step S5, the concentration of dopamine hydrochloride is changed, and the rest is the same as that in embodiment 1, and is not described herein again, and specific experimental condition parameters and performance test results are shown in the following table.

As can be seen from the above results, changing the concentration of dopamine hydrochloride in step S5 will result in Ag for the prepared orthopedic implant samples⁺The release amount and the antibacterial performance are obviously influenced, and the results of comparing examples 1, 4-5 and 6 show that the polydopamine modification is carried out on the primary orthopedic implant part modified by cyclosporine, so that the load fastness of nano silver particles is improved, the release rate is controlled, the cytotoxicity of the implant part and the rejection reaction of a matrix are reduced, and the prepared orthopedic implant sample has biocompatibility and can obtain long-term antibacterial performance.

Comparative example 1

This comparative example provides a method of manufacturing an orthopedic implant, which differs from example 1 in that: in the preparation process, the processing of step S2 is not performed, and the rest is the same as that of embodiment 1, and is not described herein again.

Comparative example 2

This comparative example provides a method of manufacturing an orthopedic implant, which differs from example 1 in that: in the preparation process, the steps S3 to S4 are not performed, and the rest is the same as that in embodiment 1, and are not described again.

Comparative example 3

This comparative example provides a method of manufacturing an orthopedic implant, which differs from example 1 in that: in the preparation process, the processing of step S6 is not performed, and the rest is the same as that of embodiment 1, and is not described herein again.

Comparative example 4

This comparative example provides a method of manufacturing an orthopedic implant, which differs from example 1 in that: the step S6 is not subjected to the ultraviolet irradiation treatment, and the rest is the same as that in embodiment 1, which is not described herein again.

The results of the performance tests on the orthopedic implant samples prepared in comparative examples 1 to 4 are shown in the following table.

As can be seen from the results of comparative example 1 and comparative examples 1 to 2, the present invention prepares a rod-shaped TiO on a porous orthopedic implant by a hydrothermal method₂Is helpful to increase the specific surface area of the porous orthopedic implant, and then modifies the immunosuppressant cyclosporine to combine the cyclosporine to the rod-shaped TiO of the primary orthopedic implant piece₂On the surface, TiO₂The rod-shaped structure greatly increases the drug loading rate of the cyclosporine, and the biocompatibility and the bacteriostatic property of the prepared orthopedic implant sample can be obviously improved through the reaction synergistic effect, so that the rejection reaction of human muscles to the orthopedic implant and the pain of a patient are reduced; the results of the comparative examples 1 and 3 show that the antibacterial property of the implant can be remarkably improved by loading the nano silver particles on the polydopamine modified orthopedic implant material; the results of comparative example 1 and comparative example 4 show that the antibacterial performance of the orthopedic implant can be synergistically improved by loading the nano silver particles on the surface of the orthopedic implant sample modified by polydopamine modification through the dipping method in cooperation with the photochemical reduction method.

While the invention has been described with respect to specific embodiments thereof, it will be understood by those skilled in the art that the foregoing and other changes, omissions and deviations in the form and detail thereof may be made without departing from the scope of this invention; those skilled in the art should appreciate that they can readily use the disclosed conception and specific embodiments as a basis for designing or modifying other structures for carrying out the same purposes of the present invention without departing from the spirit and scope of the invention; meanwhile, any equivalent changes, modifications and alterations of the above embodiments according to the spirit and techniques of the present invention are also within the scope of the present invention.

Claims

1. A method of making an orthopedic implant, comprising the steps of:

s1, preparing the porous orthopedic implant by using a 3D printing technology;

2. The method for preparing an orthopedic implant according to claim 1, wherein in step S1, the porous orthopedic implant is selected from any one of porous titanium implant, porous titanium alloy implant, porous magnesium implant, and porous magnesium alloy implant, and the pore size is 500-700 μm.

3. The method for preparing an orthopedic implant according to claim 1, wherein in step S2, the hydrothermal reaction temperature is 180 ℃ for 12 h; the high-temperature sintering treatment temperature is 500 ℃ for 3 h.

4. The method for preparing an orthopedic implant according to claim 1, wherein in step S3, the method for preparing the bonding solution is as follows: according to the mass portion, 10 portions of cyclosporine are taken and dissolved in 120 portions of 100-one deionized water, and then 1-2 portions of HBr and 0.5-0.8 portion of ZnBr are added₂Heating to 60-70 deg.C, and reacting for 60-80min to obtain binding solution.

5. The method as set forth in claim 1, wherein in step S4, ultrasonic agitation is applied intermittently with an ultrasonic agitation power of 800- "1000W and a frequency of 20-30 KHz.

6. The method for preparing an orthopedic implant according to claim 1, wherein in step S4, the distance between the cathode and the anode is controlled to be 3-5cm and the current is controlled to be 0.2-0.6A.

7. The method for preparing an orthopedic implant according to claim 1, wherein in step S5, the concentration of dopamine hydrochloride is 2-5 mg/mL.

8. The method for preparing an orthopedic implant according to claim 1, wherein in step S6, the concentration of the silver nitrate solution is 0.02-0.5 mol/L.

9. An orthopedic implant, characterized by being prepared by the preparation method of any one of claims 1 to 8.