CN110934143A

CN110934143A - Sterilization composition containing difenoconazole and fluopicolide

Info

Publication number: CN110934143A
Application number: CN201911346121.0A
Authority: CN
Inventors: 陈诺
Original assignee: Six Step Crop Protection Ltd
Current assignee: Six Step Crop Protection Ltd
Priority date: 2019-12-24
Filing date: 2019-12-24
Publication date: 2020-03-31

Abstract

The invention provides a bactericidal composition containing fluroxypyr and fluopicolide and application thereof in preventing and treating various plant diseases, wherein the ratio of the fluroxypyr to the fluopicolide is 1: 1-1: 20.

Description

Sterilization composition containing difenoconazole and fluopicolide

technical field

The invention belongs to the field of pesticides, and particularly provides a bactericidal composition containing fluroxypyr and fluopicolide and application thereof in preventing and treating various plant diseases.

Two background art

At present, with the use of a large amount of the existing bactericides on the market, the resistance of plant diseases tends to be gradually increased. Therefore, one of the trends in pesticide development is to develop a combination of agents with different action mechanisms, so as to realize synergistic interaction of the two agents and slow down the generation of disease resistance.

Chlorofluoroether azole, a novel 1 st isopropyl triazole fungicide discovered and developed by basf, has the chemical name of (2RS) -2- [4- (4-chlorophenoxy) - α -trifluoro-o-tolyl ] -1- (1H-1,2, 4-triazol-1-yl) propan-2-ol, is a C14-demethylation inhibitor that inhibits cell growth and destroys cell membrane function by preventing ergosterol biosynthesis, unlike other triazole fungicides, contains an isopropyl alcohol group in its molecule so that it can freely rotate from a free state and bind to a target to reduce mutation of germs, delay the development and development of resistance, and is relatively low in toxicity and safer for bees.

Fluopyram, the chemical name of which is 2, 6-dichloro-N- [ (3-chloro-5-trifluoromethyl-2-pyridyl) methyl ] benzamide, is a picolinamide fungicide developed by Bayer corporation. Fluopyram mainly acts on specific proteins such as spectrin between cell membranes and cell skeletons, thereby influencing mitosis of cells, having good inhibitory activity on each main form of pathogenic bacteria, having good persistence and no cross resistance of other bactericides. Fluopyram has good mobility in xylem, and can be applied to one part of a plant to protect other parts. Fluopyram is mainly used for preventing and treating common oomycete diseases such as downy mildew, epidemic disease, late blight and damping-off on various vegetables and grapes.

At present, the compounding of two bactericides of fluroxypyr and fluopicolide or the compounding of bactericides with similar mechanisms is not reported.

Disclosure of the invention

Aiming at the problems, the inventor discovers that the combination of the fluroxypyr and the fluopicolide in a certain proportion has obvious synergistic effect on cucumber downy mildew, potato late blight, rice sheath blight, pepper anthracnose, wheat rust and the like through a large number of bioassay screening tests in recent years.

In one aspect, the invention provides a bactericidal composition comprising fluroxypyr and fluopicolide.

Further, the ratio of the fluroxypyr to the fluopicolide in the bactericidal composition is 20: 1-1: 60.

further, the ratio of the fluroxypyr to the fluopicolide in the bactericidal composition is 1: 1-1: 20.

further, the ratio of the fluroxypyr to the fluopicolide in the bactericidal composition is 1: 20.

the bactericidal composition further comprises one or more components selected from solvents, emulsifiers, wetting agents, dispersants, antifreeze agents, thickeners, binders, antifoaming agents, stabilizers and fillers

Further, the formula of the bactericidal composition is as follows: 2% of fluroxypyr, 40% of fluopicolide, 10% of cyclohexanone, 2% of toluene-2, 4-diisocyanate, 4% of hexamethylene diamine, 4% of Tween-206%, 0.2% of xanthan gum, 2% of white carbon black, 4% of ethylene glycol and the balance of deionized water.

Further, the bactericidal composition is a microcapsule suspending agent, and the preparation method comprises the following steps: adding cyclohexanone into a shearing kettle, starting stirring, adding the raw medicine into the stirring kettle, stirring for 30min, adding toluene-2, 4-diisocyanate after complete dissolution, adding the reaction capsule shell, and stirring for 10min to prepare an oil phase. Cutting, adding water and emulsifier, and high-speed cutting for 30min to obtain O/W emulsion. And (4) cutting, slowly adding hexamethylene diamine while stirring, and heating and curing to obtain the preparation microcapsule. Adding ethylene glycol, white carbon black and xanthan gum into the prepared microcapsule, and stirring for 30min to prepare the microcapsule suspending agent. Filtering, sampling and inspecting to obtain the microcapsule suspending agent after being qualified.

Further, the bactericidal composition also comprises a photosynthesis enhancer.

Further, the photosynthesis enhancer is chlorella extract 0.02%.

On the other hand, the invention provides application of the bactericidal composition in preventing and treating rice sheath blight disease, grape downy mildew, tomato late blight, cucumber powdery mildew, wheat rust, cotton rhizoctonia disease, strawberry gray mold or rice blast.

The fluroxypyr and the fluopicolide can be products which meet the corresponding standards of various manufacturers, and the products can be in the forms of chemical pure products and various specific preparation products.

The solvent is one or a mixture of more than two of methyl oleate, ethyl acetate, vegetable oil, 200# solvent oil, 150# solvent oil, ethanol, n-propanol, dimethyl carbonate and the like.

The emulsifier is selected from one or a mixture of more than two of agricultural milk 400, agricultural milk 500, polymethyl acrylate, castor oil polyoxyethylene ether, fatty acid polyoxyethylene ether, polyoxyethylene fatty alcohol ether, ethoxylated castor oil, polymethyl acrylate, fatty acid polyglycol ester and the like.

The wetting agent and the dispersing agent both belong to surfactants, and can be one or a mixture of more than two of modified sodium dodecyl benzene sulfonate, sodium lauryl dimethyl polycarboxylate, fatty alcohol polyoxyethylene ether, alkylphenol ethoxylates, polyoxyethylene fatty acid, alkylaryl polyglycol ether, alkyl sulfonate, aryl sulfonate and fatty acid polyglycol.

The antifreezing agent is one or more of ethylene glycol, propylene glycol, glycerol, ethylene glycol butyl ether, propylene glycol butyl ether, ethylene glycol butyl ether acetate, dichloromethane, 1-dichloroethane, 1, 2-dichloroethane, formamide, calcium chloride, sodium acetate and magnesium chloride.

The thickening agent is one or a mixture of more than two of xanthan gum magnesium aluminum silicate, xanthan gum and carboxymethyl cellulose.

The binder is selected from one or more of glucose, sucrose, carboxyethyl cellulose, soluble starch, polyethylene glycol, methyl cellulose, polycarboxylic acids and water-soluble high molecular compound sodium carboxymethyl cellulose, polyvinyl alcohol, paraffin, gypsum, carboxypropyl methyl cellulose, polyacrylamide and the like.

The defoaming agent is one or a mixture of more than two of polytriasiloxane, polyether modified polysiloxane and polydimethylsiloxane.

The stabilizer is one or a mixture of more than two of sodium citrate, resorcinol, epoxidized soybean oil, epichlorohydrin, epoxidized linseed oil, pentaerythritol, xylitol and the like.

The filler is mainly one or a mixture of more than two of kaolin, corn starch, ammonium sulfate, light calcium, white carbon black, bentonite, gypsum, attapulgite, urea, diatomite and the like.

The photosynthesis enhancer includes, but is not limited to, trace and medium element fertilizers, organic fertilizers, amino acid fertilizers, etc., such as seaweed extract, having photosynthesis promoting effects.

The rice sheath blight disease, grape downy mildew, tomato late blight, cucumber powdery mildew, wheat rust, cotton rhizoctonia, strawberry gray mold or rice blast are not limited to diseases caused by specific strains.

The compound agent of the invention has the advantages that: (1) compared with a single agent, the bactericidal composition has obvious synergistic effect on downy mildew, late blight, sheath blight, damping off and the like, and improves the control effect; (2) the field dosage can be reduced, and the environmental pollution and pesticide residue can be effectively reduced; (3) the action mechanisms of the effective components in the bactericidal composition are different from each other, which is beneficial to avoiding and delaying the generation of drug resistance of pathogenic bacteria. (4) Compared with single medicament, the preparation method can reduce the production and use cost.

Detailed description of the invention

The following examples disclose a series of germicidal compositions and their uses. It should be noted that these examples are merely representative illustrations of the invention, and do not limit the scope of the invention. The scope of the invention is defined by the claims. Those skilled in the art can make routine adjustments to the inventive arrangements without departing from the invention.

The following examples, particularly the formulation examples, are only representative of some of the experimental results, and more of the experimental results cannot be fully demonstrated for reasons of space.

Example 1: indoor toxicity determination method for rice sheath blight by compounding fluroxypyr and fluopicolide

Test subjects: rice sheath blight disease (Rhizoctonia solani), collected in the field, and purified by indoor separation.

The test method comprises the following steps: referring to agricultural industry standard NY/T1156.5-2006 of the people's republic of China, a broad bean leaf method is adopted. Selecting a disease-susceptible broad bean pot culture, shearing leaves with the same parts, consistent growth vigor and petioles, moisturizing, and placing in a culture dish. Dissolving the raw materials with acetone, diluting with 0.1% Tween 80 water, and making into 6 series of mass concentrations. Soaking the leaves in the prepared liquid medicine for 5s, draining off excessive liquid medicine, naturally drying, inoculating the side with mycelium of fungus cake with diameter of 5mm to the center of the leaves, inoculating 30 leaves for each treatment, marking, and culturing under moisture. The test was run with a treatment containing no agent as a blank. And (5) measuring the length and width (mm) of the disease spot according to the disease condition of the blank control, and calculating the prevention and treatment effect.

The preventing and treating effect (%) is (blank control lesion diameter-medicament treatment lesion diameter) × 100/blank control lesion diameter

Calculating the inhibitory median concentration EC by least square method₅₀Then, the cotoxicity coefficient (CTC) was calculated by the Sun Yunpei method. When CTC is < 80, the composition exhibits antagonism, when 80 < CTC < 120, the composition exhibits additive effect, when CTC is>120, the composition shows a synergistic effect.

Measured virulence index (ATI) ═ standard agent EC₅₀Reagent for test EC₅₀)×100

Theoretical virulence index (TTI) ═ A agent virulence index x percent of A in the mixture + B agent virulence index x percent of B in the mixture

Co-toxicity coefficient (CTC) × 100 [ measured toxicity index (ATI) of the mixture)/Theoretical Toxicity Index (TTI) of the mixture ].

Virulence assay results are shown in table 1:

table 1 shows that the mixture ratio of the fluroxypyr to the fluopicolide is 40: 1-1: 80, the fluroxypyr and the fluopicolide have good synergistic effect on rice sheath blight, the co-toxicity coefficient is more than 120, particularly the mixture ratio of the fluroxypyr to the fluopicolide is 10: 1-1: 80, the synergistic effect is more remarkable, the co-toxicity coefficient is more than 200, wherein 1: at 20, the co-degree coefficient reaches 672.2, which is obviously higher than other mixture ratios.

Example 2: indoor toxicity determination method of fluroxypyr and fluopicolide compounded on potato late blight

Test subjects: late blight of potato

The test method comprises the following steps: the test adopts a leaf method according to agricultural industry standard NY/T1156.13-2006 of the people's republic of China.

The tested pathogen is wild potato late blight pathogen collected by Zhengzhou Mazhuang, the tested crop is a potato variety infected with potato late blight, leaves with the same leaf position and consistent growth vigor are selected on a potted seedling, the leaves are cut off from 1-2 cm of a leaf stem, the leaf stem is wrapped by a wet cotton ball and placed in a culture dish, and moisture is kept for later use.

Culturing the collected pathogenic bacteria in a suitable culture medium, washing the spore with sterile water after the spore sac is generated, filtering with double-layer gauze to obtain a spore sac suspension, and directly preparing into 1 × 10 spore sac suspension⁵sporangia/mL suspension served as inoculum.

The medicament is dissolved by acetone, diluted by 0.05 percent of Tween 80 aqueous solution, and 6 series of mass concentrations are set. And uniformly spraying the prepared liquid medicine on the back of the leaves, after the liquid medicine is naturally dried, enabling the back of each treated leaf to face upwards, and discharging the liquid medicine into a moisture preservation box according to treatment marks. Each treatment was repeated 4 times with no less than 10 leaves, and a blank was set for treatment containing acetone alone and no active ingredient.

The sporangia suspension was sprayed for inoculation. The test is a therapeutic test, typically by vaccination 24h prior to treatment with the agent. After inoculation, the leaves are continuously illuminated/darkened for 12 hours (illumination intensity is 5000 Lux-20000 Lux) alternately every day at the temperature of 18-20 ℃, water films are kept on the leaves 24 hours after inoculation, and then the leaves are cultured for 7 days under the condition that the relative humidity is more than 90%. And (5) when the blank control disease leaf rate reaches more than 50%, investigating the disease condition of each treatment in a grading way. The grading method comprises the following steps:

level 0: the disease is not developed;

level 1: only a few small disease spots are on the leaves, and the disease spots account for less than 10% of the leaf area;

and 3, level: the disease spots on the leaves occupy 10 to 25 percent of the area of the leaves;

and 5, stage: the disease spots on the leaves occupy 25 to 50 percent of the area of the leaves;

and 7, stage: the disease spots on the leaves occupy more than 50% of the area of the leaves;

and 9, stage: the whole leaves are infected with blight.

Calculating disease index and preventing and treating effect. The calculation and statistical analysis method comprises the following steps:

preventing and treating effect (%) (blank control disease index-medicament treatment disease index) × 100/blank control disease index

Co-toxicity coefficient (CTC) ═ measured toxicity index (ATI) of the mixture/Theoretical Toxicity Index (TTI) of the mixture x 100

The results of the virulence determination are shown in Table 2

Table 2 shows that the ratio of the fluroxypyr to the fluopicolide is 60: 1-1: 80, the fluroxypyr and the fluopicolide have good synergistic effect on the potato late blight, the co-toxicity coefficient is more than 120, particularly the ratio of the fluroxypyr to the fluopicolide is 20: 1-1: 80, the synergistic effect is more remarkable, the co-toxicity coefficient is more than 200, wherein 1: at 20, the co-degree coefficient reaches 540.1, which is obviously higher than other mixture ratios.

Example 3: indoor toxicity determination method for cucumber downy mildew by compounding chlorofluoromethane and fluopicolide

Test subjects: cucumber downy mildew

The test method comprises the following steps: the test adopts a plate-blade method according to agricultural industry standard NY/T1156.3-2006 of the people's republic of China.

Selecting a cucumber susceptible variety for pot culture, cutting leaves which have the same position, consistent growth vigor and 1 cm-2 cm leafstalks from top to bottom at 4-6 leaf positions, wrapping the leafstalks with wet cotton balls, placing the wrapped leafstalks in a culture dish, and preserving moisture for later use.

Selecting diseased leaf, washing the back of the leaf with distilled water at 4 deg.C to obtain suspension (concentration controlled at 1 × 10/ml)⁵One) and storing at 4 ℃ for later use.

The crude drug was dissolved in acetone and diluted with 0.05% tween 80 water. 6 series of mass concentrations were set. Uniformly spraying the liquid medicine on the back of the leaves, after the liquid medicine is naturally dried, back-facing each treated leaf, and discharging the liquid medicine in a moisture preservation box after treatment marks are formed. The test set-up contained no agent treatment as a blank.

A10. mu.L drop of fresh sporangia suspension was used to inoculate the leaf back. 4 drops were inoculated per leaf, not less than 5 leaves per treatment. The test is a therapeutic test, typically by vaccination 24h prior to treatment with the agent. And covering the inoculated seeds with a dish cover, placing the inoculated seeds in an artificial climate box, and culturing for 7d under the conditions of continuous illumination/darkness for 12h alternately every day, the temperature of 17-22 ℃ and the relative humidity of more than 90%.

Virulence assay results are shown in table 3:

table 3 shows that the ratio of the fluroxypyr to the fluopicolide is 60: 1-1: 80, the fluroxypyr and the fluopicolide have good synergistic effect on cucumber downy mildew, the co-toxicity coefficient is more than 120, particularly the ratio of the fluroxypyr to the fluopicolide is 10: 1-1: 60, the synergistic effect is more remarkable, the co-toxicity coefficient is more than 200, wherein 1: 20 and 1: when the ratio is 1, the degree of coincidence coefficient reaches over 330, which is obviously higher than other ratios.

Example 4: indoor toxicity determination method for capsicum anthracnose by compounding chlorofluoromethane and fluopicolide

Test subjects: anthracnose of hot pepper

The test method comprises the following steps: refer to agricultural industry Standard NY/T1156.2-2006 of the people's republic of China, the hypha growth rate method.

The growth rate method (plate method) was used for the experiments. On the basis of a preliminary test, quantitatively adding a pre-melted sterile culture medium into a sterile conical flask according to test treatment, sequentially taking 5ml of liquid medicine from low concentration to high concentration, respectively adding the liquid medicine into the conical flask filled with 45ml of hot culture medium (PDA culture medium, 45-50 ℃), shaking up, quickly pouring the liquid medicine into glass culture dishes with the diameter of 90mm when the culture medium is cooled to a proper temperature, and pouring 10ml of culture medium with the liquid medicine into each culture dish. Standing horizontally, cooling, and making into flat plate. Five replicates per concentration. The blank control was performed with treatment containing no active ingredient. Cutting the cultured pathogenic bacteria from the edge of the bacterial colony by using a sterilization puncher with the diameter of 4mm under aseptic conditions, inoculating the bacterial cake to the center of a drug-containing flat plate by using an inoculator, attaching the side with the hyphae downwards to a culture medium, and covering a dish cover. All the operations are carried out in a super clean bench for sterile operation. After treatment, the mixture is put into a constant-temperature sterile incubator at 28 +/-0.5 ℃ for culture and is taken out after 2 days. The colony diameters (in mm) of the respective treatments were measured by the cross method, and the average of the colony diameters was calculated. And calculating the net growth amount and the hypha growth inhibition rate of each treatment hypha.

The net growth (mm) is a measure of colony diameter-4

Hypha growth rate (%) [ (control group net growth amount-treatment group net growth amount)/control group net growth amount ] × 100

Converting the hypha growth rate into several values (y), converting the liquid medicine concentration (mug/ml) into logarithm value (x), calculating a virulence equation and an inhibitory median concentration EC50 by a least square method, and calculating the virulence index and the cotoxicity coefficient (CTC) of the medicament by a Sun cloud Pepper method. The virulence determination results are shown in table 1.

Measured virulence index (ATI) ═ standard agent EC 50/test agent EC50 x 100

When the CTC is less than or equal to 80, the composition shows antagonism, when the CTC is more than 80 and less than 120, the composition shows additive action, and when the CTC is more than or equal to 120, the composition shows synergistic action.

Virulence assay results are shown in table 4:

table 4 shows that the mixture ratio of the fluroxypyr to the fluopicolide is 60: 1-1: 80, the fluroxypyr and the fluopicolide have good synergistic effect on pepper anthracnose, the co-toxicity coefficient is more than 120, particularly the mixture ratio of the fluroxypyr to the fluopicolide is 10: 1-1: 40, the synergistic effect is more remarkable, the co-toxicity coefficient is more than 200, wherein 1: 20 and 1: when 1, the co-degree coefficient reaches about 570 and is obviously higher than other mixture ratios.

Example 5: indoor toxicity determination method for wheat rust by compounding fluroxypyr and fluopicolide

Test subjects: rust of wheat

The test method comprises the following steps: referring to agricultural industry standard NY/T1156.15-2006 of the people's republic of China, the experiment adopts a potting method.

Sowing wheat rust-infected varieties, performing pot culture until 2-4 true leaves are obtained, and numbering for later use. Using purified water with small amount of surface active substance to absorb fresh spores on wheat leaves full of powdery mildew to obtain spores with concentration of 1 × 10⁵spores/mL of spore suspension are ready for use. On the basis of the preliminary experiments, 6 series of mass concentrations were set according to the agent activity. The liquid medicine is evenly sprayed on the leaf surfaces until the leaf surfaces are completely wetted, and the liquid medicine is naturally dried for later use. Each treatment was repeated 4 times for 3 tubs and a blank was set for treatment containing only solvent and surfactant and no active ingredient. 24h after the application, rust spores are inoculated. After culturing for 7 days in a greenhouse, counting the disease spot area on each wheat seedling leaf, and calculating the disease index and the prevention and treatment effect. The grading method comprises the following steps:

level 0: the disease is not developed;

and 9, stage: the whole leaves are infected with blight.

The results of the virulence determination are shown in Table 5

Table 1 shows that the mixture ratio of the fluroxypyr to the fluopicolide is 60: 1-1: 80, the fluroxypyr and the fluopicolide have good synergistic effect on wheat rust, the co-toxicity coefficient is more than 120, particularly the mixture ratio of the fluroxypyr to the fluopicolide is 10: 1-1: 40, the synergistic effect is more remarkable, the co-toxicity coefficient is more than 200, wherein 1: at 20, the co-degree coefficient reaches 360.1, which is obviously higher than other mixture ratios.

Example 6: 22% Fluroxypyr-Fluopyram suspension

The active ingredient raw drug, the auxiliary agent and other components are uniformly mixed according to the proportion of the formula, and the 22 percent of fluroxypyr-meptyl-fluopicolide suspending agent is obtained after grinding and/or high-speed shearing.

The embodiment is applied to the prevention and treatment of rice sheath blight disease. The 22% of the fluroxypyr-mepiquat chloride-fluopicolide suspending agent is diluted by water to 1000 times and sprayed, and the control effects of the fluroxypyr-mepiquat chloride-fluopicolide suspending agent in 7 days and 15 days after the spraying are 93.5% and 95.7% respectively. The 25% fluroxypyr suspension is 1000 times and the 50% flupyrad water dispersible granule is 2000 times, the same method is used, the control effect is 63.8% and 59.7% respectively after the medicine is taken for 7 days, and the control effect is 72.9% and 65.3% after the medicine is taken for 15 days. After the fluroxypyr and the fluopicolide are compounded, the synergistic effect is obvious, the control effect on rice sheath blight is obviously better than that of a single agent, the control effect is improved, the using amount of active ingredients is obviously reduced compared with that of the active ingredients when the fluroxypyr and the fluopicolide are used alone, and the pollution to the environment is reduced.

Example 7: 30% chlorofluoromethrizole-fluopicolide microemulsion

Putting the raw medicines into a stirring kettle, stirring for a specified time, adding an emulsifier and other auxiliary agents, stirring again to prepare an oil phase, adding water while shearing until the water is completely added, shearing for 30min, filtering, sampling, inspecting, discharging after the product is qualified, and obtaining the 30% chlorofluoroether conazole-fluopicolide microemulsion.

This example applies to the control of grape downy mildew. The 30% of the microemulsion of the fluroxypyr and the fluopicolide is diluted by water by 1000 times for spraying, and the control effects of the microemulsion after being applied for 7 days and 15 days are respectively 92.1% and 95.2%. The 25% fluroxypyr suspension is 1000 times and the 50% fluopicolide water dispersible granule is 2000 times, the same method is used, the control effect is 73.4% and 65.9% respectively 7 days after the application, and 78.8% and 72.0% 15 days after the application. After the fluroxypyr and the fluopicolide are compounded, the synergistic effect is obvious, the control effect on the grape downy mildew is obviously better than that of a single agent, the control effect is improved, the using amount of the effective components is obviously reduced compared with that of the effective components when the fluroxypyr and the fluopicolide are used alone, and the pollution to the environment is reduced.

Example 8: 30% aqueous dispersion granule of fluroxypyr-meptyl-fluopicolide

According to the specified formula, the original drug, the auxiliary agent and the filler are put into a conical mixer, are stirred for 30min, are uniformly mixed, enter a jet mill for jet milling, enter the conical mixer again for secondary mixing, are stirred for 30min, are uniformly mixed for the second time, are sampled and inspected, and are qualified, so that the 30% chlorofluoroether triflumizole-fluopicolide water dispersible granule is obtained.

The embodiment is applied to the control of tomato late blight. 30 percent of the aqueous dispersion granule of the fluroxypyr-mepiquat chloride and the fluopicolide is diluted by water to 1000 times and sprayed, and the control effects of the mixture in 7 days and 15 days after the application are 91.9 percent and 94.5 percent respectively. The 25% fluroxypyr suspension concentrate is 1000 times and the 50% fluopicolide water dispersible granule is 2000 times, the same method is used, the control effect is 69.2% and 63.4% respectively after the drug is applied for 7 days, and 80.9% and 76.8% after the drug is applied for 15 days. The synergistic effect is obvious after the fluroxypyr and the fluopicolide are compounded, the control effect on the tomato late blight is obviously better than that of a single agent, the control effect is improved, the using amount of active ingredients is obviously reduced compared with that of the active ingredients when the active ingredients are used alone, and the pollution to the environment is reduced.

Example 9: 15% fluroxypyr-mepiquat chloride-fluopicolide soluble solution

Accurately weighing the original medicine, the auxiliary agent and the solvent according to the requirement of the formula, putting the original medicine, the auxiliary agent and the solvent into a stirring kettle, fully stirring, uniformly mixing, extracting a sample, and obtaining the 15 percent soluble solution of the fluroxypyr-meprazole and fluopyram after the sample is detected to be qualified.

The embodiment is applied to controlling cucumber powdery mildew. The 15 percent of the fluroxypyr-mepiquat chloride-fluopicolide soluble solution is diluted by water by 1000 times and sprayed, and the control effect is 89.6 percent and 93.7 percent respectively after 7 days and 15 days of spraying. The 25% fluroxypyr suspension is 1000 times and the 50% flupyrad water dispersible granule is 2000 times, the same method is used, the control effect is 52.9% and 60.6% respectively after the drug is applied for 7 days, and the control effect is 59.3% and 67.2% after the drug is applied for 15 days. The synergistic effect is obvious after the fluroxypyr and the fluopicolide are compounded, the control effect on the cucumber powdery mildew is obviously better than that of a single agent, the control effect is improved, the using amount of the effective components is obviously reduced compared with that of the effective components when the fluroxypyr and the fluopicolide are used alone, and the pollution to the environment is reduced.

Example 10: 20% of fluroxypyr-mepiquat chloride-fluopicolide missible oil

According to the specified formula, firstly putting cyclohexanone and No. 150 solvent oil into a stirring kettle, starting stirring, then putting the original drug into the stirring kettle, stirring for 30min, finally adding other auxiliary agents, stirring for 30min, standing, filtering, sampling and inspecting, and obtaining the 20% chlorofluoroether ipconazole-fluopicolide missible oil after the product is qualified.

This example is applied to control wheat rust. The 20 percent of the fluroxypyr-mepiquat chloride-fluopicolide missible oil is diluted by water by 1000 times and sprayed, and the control effects of the fluroxypyr-mepiquat chloride-fluopicolide missible oil in 7 days and 15 days after the spraying are respectively 92.7 percent and 94.9 percent. The 25% fluroxypyr suspension is 1000 times and the 50% flupyrad water dispersible granule is 2000 times, the same method is used, the control effect is 72.9% and 69.8% respectively after the drug is applied for 7 days, and the control effect is 81.5% and 80.4% after the drug is applied for 15 days. After the fluroxypyr and the fluopicolide are compounded, the synergistic effect is obvious, the control effect on wheat rust is obviously better than that of a single agent, the control effect is improved, the using amount of active ingredients is obviously reduced compared with that of the active ingredients when the fluroxypyr and the fluopicolide are used alone, and the pollution to the environment is reduced.

Example 11: 26% Chlorofloxacin Fluopyram suspoemulsion

Uniformly mixing all components of part of the auxiliary agent according to the proportion of the formula, grinding and/or shearing at a high speed to obtain a suspending agent, directly emulsifying the original drug and other auxiliary agents into a suspending agent by using a high-speed stirrer, sampling, inspecting, and obtaining the 26% chlorofluoroether conazole-fluopicolide suspending agent after the suspending agent is qualified.

The embodiment is applied to preventing and treating cotton rhizoctonia rot. The 26 percent of the suspension emulsion of the fluroxypyr and the fluopicolide is diluted by water to 1000 times for spraying, and the control effects of the suspension emulsion for 7 days and 15 days after the application are respectively 88.2 percent and 92.1 percent. The 25% fluroxypyr suspension is 1000 times and the 50% flupyrad water dispersible granule is 2000 times, the same method is used, the control effect is respectively 59.9% and 62.4% after the drug is applied for 7 days, and the control effect is 72.3% and 74.6% after the drug is applied for 15 days. The synergistic effect is obvious after the fluroxypyr and the fluopicolide are compounded, the control effect on cotton rhizoctonia is obviously better than that of a single agent, the control effect is improved, the using amount of active ingredients is obviously reduced compared with that of the active ingredients when the fluroxypyr and the fluopicolide are used alone, and the pollution to the environment is reduced.

Example 12: 33% fluroxypyr-mepiquat chloride-fluopicolide aqueous emulsion

Adding solvent into a shearing kettle, stirring, adding the raw medicine into the shearing kettle, stirring for a specified time, adding other additives such as emulsifier, stirring again to obtain an oil phase, shearing while adding water until the water is completely added, shearing for 30min, filtering, sampling, inspecting, and discharging after qualification to obtain 33% chlorofluoroether azole-fluopicolide water emulsion.

The embodiment is applied to preventing and controlling the gray mold of the strawberry. The 33% of the fluroxypyr-mepiquat chloride-fluopicolide water emulsion is diluted by water to 1000 times and sprayed, and the control effect is 93.1% and 96.3% respectively after 7 days and 15 days of application. The 25% fluroxypyr suspension is 1000 times and the 50% fluopicolide water dispersible granule is 2000 times, the same method is used, the control effect is 80.5% and 74.3% respectively after the drug is applied for 7 days, and the control effect is 85.2% and 81.7% after the drug is applied for 15 days. The synergistic effect is obvious after the fluroxypyr and the fluopicolide are compounded, the control effect on the gray mold of the strawberries is obviously better than that of a single agent, the control effect is improved, the using amount of active ingredients is obviously reduced compared with that of the active ingredients when the fluroxypyr and the fluopicolide are used alone, and the pollution to the environment is reduced.

Example 13: 42% fluroxypyr-mepiquat chloride-fluopicolide microcapsule suspending agent

Adding cyclohexanone into a shearing kettle, starting stirring, adding the raw medicine into the stirring kettle, stirring for 30min, adding toluene-2, 4-diisocyanate after complete dissolution, adding the reaction capsule shell, and stirring for 10min to prepare an oil phase. Cutting, adding water and emulsifier, and high-speed cutting for 30min to obtain O/W emulsion. And (4) cutting, slowly adding hexamethylene diamine while stirring, and heating and curing to obtain the preparation microcapsule. Adding ethylene glycol, white carbon black and xanthan gum into the prepared microcapsule, and stirring for 30min to prepare the microcapsule suspending agent. Filtering, sampling and inspecting to obtain the 42 percent fluroxypyr-mepiquat chloride-fluopicolide microcapsule suspending agent after the components are qualified.

This example was applied to control of rice blast. 42 percent of the fluroxypyr-mepiquat chloride-fluopicolide microcapsule suspending agent is diluted by water to 1000 times and sprayed, and the control effects of the spraying agent in 7 days and 15 days are respectively 92.5 percent and 94.8 percent. The 25% fluroxypyr suspension is 1000 times and the 50% flupyrad water dispersible granule is 2000 times, the same method is used, the control effect is 72.3% and 75.9% respectively after the medicine is taken for 7 days, and 82.9% and 84.7% after the medicine is taken for 15 days. After the fluroxypyr and the fluopicolide are compounded, the synergistic effect is obvious, the rice blast prevention effect is obviously better than that of a single agent, the prevention and treatment effect is improved, the using amount of the effective components is obviously reduced compared with that of the effective components when the fluroxypyr and the fluopicolide are used alone, and the pollution to the environment is reduced.

Microemulsion and suspoemulsion of fluopicolide 40%, fluroxypyr 2% and fluroxypyr 42% fluroxypyr-mepiquat chloride are prepared according to the methods of example 7 and example 11, and diluted 1000-fold with water for spraying, and the rice blast control effect is 86.5% and 87.8% respectively 7 days and 15 days after application.

Example 14 application of photosynthesis enhancer

To the formulations of examples 6-13, it was attempted to add 0.02% of photosynthesis enhancer (chlorella extract) to further increase crop yield and extend the duration of action, increasing the duration of action from around 25 days to 35 days for rice blast, wheat rust and tomato late blight.

Claims

1. A bactericidal composition comprising fluroxypyr and fluopicolide.

2. The fungicidal composition according to claim 1, wherein the ratio of fluroxypyr to fluopicolide is 20: 1-1: 60.

3. the fungicidal composition according to claim 2, wherein the ratio of fluroxypyr to fluopicolide is 1: 1-1: 20.

4. the fungicidal composition according to claim 3, wherein the ratio of fluroxypyr to fluopicolide is 1: 20.

5. the fungicidal composition according to any one of claims 1 to 4, further comprising an ingredient selected from one or more of a solvent, an emulsifier, a wetting agent, a dispersant, an antifreeze, a thickener, a binder, an antifoaming agent, a stabilizer, and a filler.

6. The germicidal composition of claim 5, formulated as: 2% of fluroxypyr, 40% of fluopicolide, 10% of cyclohexanone, 2% of toluene-2, 4-diisocyanate, 4% of hexamethylene diamine, 4% of Tween-206%, 0.2% of xanthan gum, 2% of white carbon black, 4% of ethylene glycol and the balance of deionized water.

7. The germicidal composition according to claim 6, which is a suspension of microcapsules, and is prepared by: adding cyclohexanone into a shearing kettle, starting stirring, adding the raw medicine into the stirring kettle, stirring for 30min, adding toluene-2, 4-diisocyanate after complete dissolution, adding the reaction capsule shell, and stirring for 10min to prepare an oil phase. Cutting, adding water and emulsifier, and high-speed cutting for 30min to obtain O/W emulsion. And (4) cutting, slowly adding hexamethylene diamine while stirring, and heating and curing to obtain the preparation microcapsule. Adding ethylene glycol, white carbon black and xanthan gum into the prepared microcapsule, and stirring for 30min to prepare the microcapsule suspending agent. Filtering, sampling and inspecting to obtain the microcapsule suspending agent after being qualified.

8. The fungicidal composition according to any one of claims 1 to 7, further comprising a photosynthesis enhancer.

9. The bactericidal composition according to claim 8, wherein the photosynthesis enhancer is chlorella extract 0.02%.

10. The use of a fungicidal composition according to any of claims 1 to 9 for the control of rice sheath blight, grape downy mildew, tomato late blight, cucumber powdery mildew, wheat rust, cotton rhizoctonia, strawberry gray mold or rice blast.