CN110927277A - Method for rapidly detecting concentrations of vitamin B1 and vitamin C based on liquid chromatography-tandem mass spectrometry technology - Google Patents
Method for rapidly detecting concentrations of vitamin B1 and vitamin C based on liquid chromatography-tandem mass spectrometry technology Download PDFInfo
- Publication number
- CN110927277A CN110927277A CN201911234804.7A CN201911234804A CN110927277A CN 110927277 A CN110927277 A CN 110927277A CN 201911234804 A CN201911234804 A CN 201911234804A CN 110927277 A CN110927277 A CN 110927277A
- Authority
- CN
- China
- Prior art keywords
- vitamin
- concentrations
- solution
- mass spectrometry
- liquid chromatography
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/86—Signal analysis
- G01N30/8675—Evaluation, i.e. decoding of the signal into analytical information
- G01N30/8679—Target compound analysis, i.e. whereby a limited number of peaks is analysed
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N2030/042—Standards
- G01N2030/045—Standards internal
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Library & Information Science (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The invention discloses a method for rapidly detecting concentrations of vitamin B1 and vitamin C based on a liquid chromatography-tandem mass spectrometry technology, which comprises the steps of preparing a vitamin B1 and vitamin C reference solution and a vitamin B1 and vitamin C isotope internal standard solution; establishing a standard curve of vitamin B1 and vitamin C; collecting a chromatogram of a serum sample solution; determination of vitamin B1 and vitamin C concentrations in serum samples. The detection method disclosed by the invention is simple, sensitive, rapid and accurate, strong in specificity and good in repeatability, and can be used for rapidly detecting vitamin B1 and vitamin C in serum.
Description
Technical Field
The invention relates to a detection method of water-soluble vitamins, in particular to a rapid detection method of vitamin B1 and vitamin C concentration based on a liquid chromatography-tandem mass spectrometry technology.
Background
Vitamins, also known as vitamins, are nutrients that maintain life. Plays an important role in the growth, development and metabolism of human bodies, and the substances cannot be synthesized in the bodies or the synthesis amount is insufficient, so the substances must be continuously supplied from the outside. Vitamins are classified into two major classes, fat-soluble vitamins and water-soluble vitamins, according to their structure and solubility. Water-soluble vitamins refer to vitamins that are soluble in water, usually as a component of a coenzyme or prosthetic group, including B vitamins and vitamin C.
The invention has the following patent: 2016105698131, a method for rapidly determining vitamin B1 and vitamin C in multi-vitamin tablets. The multi-dimensional film is detected, but the detection method combines various technologies, is complex, has long detection time, and can be seen from the graph for at least 20 min.
The existing methods for detecting the concentrations of vitamin B1 and vitamin C are generally based on ion mass spectrometry, the detection time is long, the result is unstable, and a detection method capable of quickly, simply, accurately and efficiently realizing the concentrations of vitamin B1 and vitamin C is urgently needed.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: how to quickly and accurately measure the concentration of various vitamins provides a method for quickly detecting the concentration of vitamin B1 and vitamin C based on a liquid chromatography-tandem mass spectrometry technology.
The invention solves the technical problems through the following technical scheme, and the invention comprises the following steps:
(1) preparing vitamin B1 and vitamin C reference solution and vitamin B1 and vitamin C isotope internal standard solution;
preparing reference stock solutions of vitamin B1 and vitamin C with known concentrations and isotope internal standard stock solutions of vitamin B1 and vitamin C with known concentrations;
then diluting the vitamin B1 and vitamin C reference stock solution to obtain a mixed reference solution of vitamin B1 and vitamin C with known concentrations, and further diluting to obtain vitamin B1 and vitamin C reference working solutions with different known concentrations;
diluting vitamin B1 and vitamin C isotope internal standard stock solution into mixed internal standard solution of vitamin B1 and vitamin C with known concentration;
(2) establishing a standard curve of vitamin B1 and vitamin C; respectively sucking a series of mixed reference substance working solutions of vitamin B1 and vitamin C with different known concentrations, and respectively adding mixed internal standard solutions of vitamin B1 and vitamin C to prepare a series of mixed standard curve working solutions of vitamin B1 and vitamin C with different known concentrations;
then detecting mixed standard curve working solution of vitamin B1 and vitamin C with different known concentrations under the same preset condition of ultra performance liquid chromatography tandem mass spectrometry to obtain chromatograms and peak areas of vitamin B1 and vitamin C, vitamin B1 and vitamin C isotope internal standards with different known concentrations,
establishing a standard curve of vitamin B1 and vitamin C by taking the ratio of the vitamin B1 peak area to the vitamin B1 isotope internal standard peak area and the ratio of the vitamin C peak area to the vitamin C isotope internal standard peak area in a series of mixed standard curve working solutions as the ordinate or the abscissa and taking the concentrations of vitamin B1 and vitamin C in corresponding vitamin B1 and vitamin C standard curve working solutions as the abscissa or the ordinate;
(3) collecting a chromatogram of a serum sample solution;
adding a mixed internal standard solution of vitamin B1 and vitamin C into the serum sample to obtain a serum sample solution, and obtaining a chromatogram and a peak area of the serum sample solution under the same ultra performance liquid chromatography tandem mass spectrometry condition as the step (2);
(4) determination of vitamin B1 and vitamin C concentrations in serum samples
And respectively bringing the ratio of the vitamin B1 peak area to the vitamin B1 isotope internal standard peak area and the ratio of the vitamin C peak area to the vitamin C isotope internal standard peak area in the chromatogram of the serum sample solution into the established vitamin B1 and vitamin C standard curves, and calculating the concentrations of the vitamin B1 and the vitamin C in the serum sample.
In the step (1):
dissolving vitamin B1 and vitamin C reference substances and vitamin B1 and vitamin C isotope internal standards respectively by using a methanol aqueous solution containing an antioxidant with the volume fraction of 50% to prepare a vitamin B1 and vitamin C reference substance stock solution with known concentrations and a vitamin B1 and vitamin C isotope internal standard stock solution with known concentrations;
diluting a vitamin B1 and vitamin C reference stock solution with known concentration into a vitamin B1 and vitamin C mixed reference solution with known concentration by using a methanol water solution with 50% of volume fraction of antioxidant, and further diluting the vitamin B1 and vitamin C mixed reference solution into a series of vitamin B1 and vitamin C reference working solutions with different known concentration by using a methanol water solution with 50% of volume fraction of antioxidant;
and diluting the isotope internal standard stock solution of vitamin B1 and vitamin C with known concentration by using methanol containing antioxidant into mixed internal standard solution of vitamin B1 and vitamin C with known concentration.
The antioxidant is at least one selected from sodium sulfite, sodium pyrosulfite, dithiothreitol, sodium bisulfite, sodium thiosulfate and dibutylphenol.
In the step (2), respectively absorbing a series of mixed reference substance working solutions of vitamin B1 and vitamin C with different known concentrations, adding mixed internal standard solutions of vitamin B1 and vitamin C with a volume ratio of 1: 1-5, uniformly mixing in a vortex mode, centrifuging, taking supernate, adding pure water with a volume ratio of 1: 1-10, and mixing in a vortex mode to obtain a series of mixed standard curve working solutions of vitamin B1 and vitamin C with different known concentrations; the centrifugation conditions were: centrifuging at 10000-14000 rpm at 2-8 ℃ for 5-20 min.
The vitamin B1 and vitamin C isotope internal standards are 13C or 2H labeled vitamin B1 and vitamin C. The accuracy and repeatability of the isotope internal standard are higher than those of the non-isotope internal standard, and the isotope internal standard is adopted to improve the accuracy and repeatability of detection.
The conditions of the ultra-high performance liquid chromatography tandem mass spectrometry are as follows:
ultra-high performance liquid chromatography conditions:
a chromatographic column: a reverse phase chromatography column; column temperature: 30-60 ℃; flow rate: 0.3-0.8 mL/min; mobile phase system: a water-methanol system adopts gradient elution;
the mass spectrometry conditions include:
an ion source: electrospray ion source, positive ion mode; scanning mode: multiple reactions monitor MRM mode.
The reversed phase chromatographic column is a polar group embedded C18 chromatographic column, the filler particle size is 1.7-5.0 mu m, the inner diameter is 2.1-4.6 mm, and the column length is 25-150 mm.
The mobile phase system is a 0.1% formic acid aqueous solution of the phase A to a 0.1% formic acid methanol solution of the phase B.
The gradient elution conditions were: 0-0.8 min: 5-30% of B, 0.8-1 min: 30-100% B, 1-1.2 min: 100-5% of B, 1.2-2 min: 5% of B.
The condition of the multi-stage reaction monitoring MRM mode is as follows: vitamin B1, m/z265.03> 121.98; vitamin B1-13C4, m/z 269.10> 121.99; vitamin C, m/z 177.03>94.93, vitamin C-13C6, m/z 183.03> 99.96.
Compared with the prior art, the invention has the following advantages: the detection method is simple, sensitive, rapid and accurate, has strong specificity and good repeatability, can be used for rapidly detecting the vitamin B1 and the vitamin C in the serum, can directly detect biological samples by adopting a mass spectrometry method, can detect each sample in only 2min, can operate nearly thousands of samples in 24h, and greatly improves the detection time and effect.
Drawings
FIG. 1 is a typical LC-MS/MS chromatogram of a vitamin B1 and vitamin C mixed standard curve working solution;
FIG. 2 is a typical LC-MS/MS chromatogram of a serum sample.
Detailed Description
The following examples are given for the detailed implementation and specific operation of the present invention, but the scope of the present invention is not limited to the following examples.
The specific detection process of this embodiment is as follows:
first, standard substance preparation
1. Preparing a standard substance stock solution: precisely weighing appropriate amount of vitamin B1 and vitamin C standard substance, respectively placing in 10mL volumetric flasks, dissolving with 50% methanol aqueous solution of dithiothreitol 10mg/mL, and fixing volume to scale, to obtain vitamin B1 and vitamin C stock solution with concentration of 0.787mg/mL and 0.945mg/mL respectively. Dissolving with 10mg/mL dithiothreitol 50% methanol aqueous solution, transferring vitamin B1-13C4 and vitamin C-13C6 into 10mL volumetric flasks, and making into vitamin B1-13C4 and vitamin C-13C6 stock solutions with concentrations of 0.1mg/mL and 0.2mg/mL respectively.
2. Preparation of mixed standard solution: precisely transferring a proper amount of vitamin B1 and vitamin C stock solution, and adding 10mg/mL of dithiothreitol 50% methanol aqueous solution to obtain vitamin B1 and vitamin C mixed working standard solutions with the concentrations of 78.7ng/mL and 37.8 mu g/mL respectively; the mixed standard solution is diluted by a 50% methanol aqueous solution of dithiothreitol with the concentration of 10mg/mL in a multiple ratio to obtain a series of mixed standard solutions with different concentrations, and the specific concentrations are shown in Table 1.
TABLE 1 concentration of mixed standard solutions
| Standard solution | VB1(ng/mL) | VC(μg/mL) |
| Std1 | 78.70 | 37.80 |
| Std2 | 39.35 | 18.90 |
| Std3 | 19.68 | 9.450 |
| Std4 | 7.870 | 3.780 |
| Std5 | 3.935 | 1.890 |
| Std6 | 1.968 | 0.945 |
| Std7 | 0.787 | 0.378 |
| Std8 | 0.394 | 0.189 |
3. Preparation of mixed internal standard solution: precisely transferring a proper amount of vitamin B1-13C4 and vitamin C-13C6 stock solutions, and adding 10mg/mL dithiothreitol methanol to obtain mixed internal standard solutions of vitamin B1-13C4 and vitamin C-13C6 with the concentrations of 10ng/mL and 2 mug/mL respectively.
Secondly, sample preparation:
and adding 200 mul of mixed internal standard solution into 100 mul of serum or standard solution, uniformly mixing by vortex for 3min, centrifuging for 10min at 14000rpm at 4 ℃, taking 50 mul of supernatant, adding 150 mul of pure water, and mixing by vortex for 1min to obtain the sample to be detected.
Third, liquid chromatogram tandem mass spectrum detection condition
1. Chromatographic conditions are as follows: a chromatographic column: acquity UPLC HSS T3 (2.1X 50mm,1.8 μm); column temperature: 50 ℃; mobile phase: and (3) carrying out gradient elution from 0.1% formic acid aqueous solution (A) to 0.1% formic acid methanol solution (B), wherein the specific gradient elution conditions are shown in Table 2:
TABLE 2 gradient elution conditions
2. Mass spectrum conditions: an ion source: electrospray ion source, positive ion mode; capillary voltage: 3.0 kV; ion source temperature: 150 ℃; desolventizing temperature: 550 ℃; removing the solvent gas: 700L/Hr; taper hole gas: 50L/Hr; scanning mode: the MRM mode, specific MRM conditions, are shown in table 3:
TABLE 3 MRM Condition for the Compounds
| Compound (I) | Ion channel | Taper hole voltage (V) | Collision voltage (V) |
| Vitamin B1(VB1) | 265.03>121.98 | 22 | 18 |
| Vitamin C (VC) | 177.03>94.93 | 20 | 10 |
| Vitamin B1-13C4(VB1-13C4) | 269.10>121.99 | 22 | 14 |
| Vitamin C-13C6(VC-13C6) | 183.03>99.96 | 18 | 12 |
Fourth, data analysis
Under the conditions of the liquid chromatography tandem mass spectrometry detection, chromatograms of vitamin B1 and vitamin C standards and serum samples are shown in FIGS. 1 and 2. The retention time of vitamin B1 and vitamin C is 0.55 min and 0.66min respectively.
And (3) carrying out quantitative analysis by adopting an internal standard method, namely taking the ratio of the peak area of each compound to the peak area of the corresponding internal standard as a dependent variable (y) and substituting the dependent variable into a linear regression equation to calculate the concentration.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (10)
1. A method for rapidly detecting the concentrations of vitamin B1 and vitamin C based on a liquid chromatography-tandem mass spectrometry technology is characterized by comprising the following steps:
(1) preparing vitamin B1 and vitamin C reference solution and vitamin B1 and vitamin C isotope internal standard solution;
preparing reference stock solutions of vitamin B1 and vitamin C with known concentrations and isotope internal standard stock solutions of vitamin B1 and vitamin C with known concentrations;
then diluting the vitamin B1 and vitamin C reference stock solution to obtain a mixed reference solution of vitamin B1 and vitamin C with known concentrations, and further diluting to obtain vitamin B1 and vitamin C reference working solutions with different known concentrations;
diluting vitamin B1 and vitamin C isotope internal standard stock solution into mixed internal standard solution of vitamin B1 and vitamin C with known concentration;
(2) establishing a standard curve of vitamin B1 and vitamin C;
respectively sucking a series of mixed reference substance working solutions of vitamin B1 and vitamin C with different known concentrations, and respectively adding mixed internal standard solutions of vitamin B1 and vitamin C to prepare a series of mixed standard curve working solutions of vitamin B1 and vitamin C with different known concentrations;
then detecting mixed standard curve working solution of vitamin B1 and vitamin C with different known concentrations under the same preset condition of ultra performance liquid chromatography tandem mass spectrometry to obtain chromatograms and peak areas of vitamin B1 and vitamin C, vitamin B1 and vitamin C isotope internal standards with different known concentrations,
establishing a standard curve of vitamin B1 and vitamin C by taking the ratio of the vitamin B1 peak area to the vitamin B1 isotope internal standard peak area and the ratio of the vitamin C peak area to the vitamin C isotope internal standard peak area in a series of mixed standard curve working solutions as the ordinate or the abscissa and taking the concentrations of vitamin B1 and vitamin C in corresponding vitamin B1 and vitamin C standard curve working solutions as the abscissa or the ordinate;
(3) collecting a chromatogram of a serum sample solution;
adding a mixed internal standard solution of vitamin B1 and vitamin C into the serum sample to obtain a serum sample solution, and obtaining a chromatogram and a peak area of the serum sample solution under the same ultra performance liquid chromatography tandem mass spectrometry condition as the step (2);
(4) determination of vitamin B1 and vitamin C concentrations in serum samples
And respectively bringing the ratio of the vitamin B1 peak area to the vitamin B1 isotope internal standard peak area and the ratio of the vitamin C peak area to the vitamin C isotope internal standard peak area in the chromatogram of the serum sample solution into the established vitamin B1 and vitamin C standard curves, and calculating the concentrations of the vitamin B1 and the vitamin C in the serum sample.
2. The method for rapidly detecting the concentrations of vitamin B1 and vitamin C based on the liquid chromatography-tandem mass spectrometry technology as claimed in claim 1, wherein in the step (1):
dissolving vitamin B1 and vitamin C reference substances and vitamin B1 and vitamin C isotope internal standards respectively by using a methanol aqueous solution containing an antioxidant with the volume fraction of 50% to prepare a vitamin B1 and vitamin C reference substance stock solution with known concentrations and a vitamin B1 and vitamin C isotope internal standard stock solution with known concentrations;
diluting a vitamin B1 and vitamin C reference stock solution with known concentration into a vitamin B1 and vitamin C mixed reference solution with known concentration by using a methanol water solution with 50% of volume fraction of antioxidant, and further diluting the vitamin B1 and vitamin C mixed reference solution into a series of vitamin B1 and vitamin C reference working solutions with different known concentration by using a methanol water solution with 50% of volume fraction of antioxidant;
and diluting the isotope internal standard stock solution of vitamin B1 and vitamin C with known concentration by using methanol containing antioxidant into mixed internal standard solution of vitamin B1 and vitamin C with known concentration.
3. The method for rapidly detecting the concentrations of vitamin B1 and vitamin C according to claim 2, wherein the antioxidant is at least one selected from the group consisting of sodium sulfite, sodium metabisulfite, dithiothreitol, sodium bisulfite, sodium thiosulfate and dibutylphenol.
4. The method for rapidly detecting the concentrations of vitamin B1 and vitamin C based on the liquid chromatography tandem mass spectrometry technology as claimed in claim 1, wherein in the step (2), a series of mixed reference substance working solutions of vitamin B1 and vitamin C with different known concentrations are respectively sucked, mixed internal standard solutions of vitamin B1 and vitamin C with a volume ratio of 1: 1-5 are added, vortex mixing is performed, centrifugation is performed, supernatant is taken, pure water with a volume ratio of 1: 1-10 is added, and vortex mixing is performed to obtain a series of mixed standard curve working solutions of vitamin B1 and vitamin C with different known concentrations; the centrifugation conditions were: centrifuging at 10000-14000 rpm at 2-8 ℃ for 5-20 min.
5. The method for rapidly detecting the concentrations of vitamin B1 and vitamin C based on the liquid chromatography-tandem mass spectrometry technology as claimed in claim 1, wherein the isotope internal standards of vitamin B1 and vitamin C are 13C or 2H labeled vitamin B1 and vitamin C.
6. The method for rapidly detecting the concentrations of vitamin B1 and vitamin C based on the liquid chromatography-tandem mass spectrometry technology as claimed in claim 1, wherein the conditions of the ultra performance liquid chromatography-tandem mass spectrometry are as follows:
ultra-high performance liquid chromatography conditions:
a chromatographic column: a reverse phase chromatography column; column temperature: 30-60 ℃; flow rate: 0.3-0.8 mL/min; mobile phase system: a water-methanol system adopts gradient elution;
the mass spectrometry conditions include:
an ion source: electrospray ion source, positive ion mode; scanning mode: multiple reactions monitor MRM mode.
7. The method for rapidly detecting the concentrations of vitamin B1 and vitamin C based on the liquid chromatography-tandem mass spectrometry technology as claimed in claim 6, wherein the reversed phase chromatographic column is a polar group embedded C18 chromatographic column, the filler particle size is 1.7-5.0 μm, the inner diameter is 2.1-4.6 mm, and the column length is 25-150 mm.
8. The method for rapidly detecting the concentrations of vitamin B1 and vitamin C based on the liquid chromatography-tandem mass spectrometry technology as claimed in claim 6, wherein the mobile phase system is from A phase 0.1% formic acid aqueous solution to B phase 0.1% formic acid methanol solution.
9. The method for rapidly detecting the concentration of vitamin B1 and vitamin C based on the liquid chromatography-tandem mass spectrometry technology as claimed in claim 6, wherein the gradient elution conditions are as follows: 0-0.8 min: 5-30% of B, 0.8-1 min: 30-100% B, 1-1.2 min: 100-5% of B, 1.2-2 min: 5% of B.
10. The method for rapidly detecting the concentrations of vitamin B1 and vitamin C based on the liquid chromatography-tandem mass spectrometry technology as claimed in claim 6, wherein the conditions for monitoring the MRM mode by the multistage reaction are as follows: vitamin B1, m/z265.03> 121.98; vitamin B1-13C4, m/z 269.10> 121.99; vitamin C, m/z 177.03>94.93, vitamin C-13C6, m/z 183.03> 99.96.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911234804.7A CN110927277A (en) | 2019-12-05 | 2019-12-05 | Method for rapidly detecting concentrations of vitamin B1 and vitamin C based on liquid chromatography-tandem mass spectrometry technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911234804.7A CN110927277A (en) | 2019-12-05 | 2019-12-05 | Method for rapidly detecting concentrations of vitamin B1 and vitamin C based on liquid chromatography-tandem mass spectrometry technology |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110927277A true CN110927277A (en) | 2020-03-27 |
Family
ID=69857051
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911234804.7A Pending CN110927277A (en) | 2019-12-05 | 2019-12-05 | Method for rapidly detecting concentrations of vitamin B1 and vitamin C based on liquid chromatography-tandem mass spectrometry technology |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110927277A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114527230A (en) * | 2022-02-28 | 2022-05-24 | 上海药明奥测医疗科技有限公司 | Method for detecting concentration of vitamin A and vitamin E in human dry blood spots |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120048003A1 (en) * | 2010-08-24 | 2012-03-01 | GOLDEN PRODUCTS LLC, Limited Liability Company | Vitamin assay methods |
| CN105424854A (en) * | 2015-11-23 | 2016-03-23 | 济南英盛生物技术有限公司 | Method for detecting various water-soluble vitamins in blood sample at same time |
| CN106248773A (en) * | 2016-07-19 | 2016-12-21 | 中国检验检疫科学研究院 | Vitamin B in a kind of quickly mensuration multidimensional sheet1with ascorbic method |
| CN106442838A (en) * | 2016-10-19 | 2017-02-22 | 杭州佰辰医学检验所有限公司 | Isotope dilution ultra-high performance liquid chromatography-mass spectrometry method for measuring vitamin B1 in serum |
| CN110412175A (en) * | 2019-09-05 | 2019-11-05 | 北京和合医学诊断技术股份有限公司 | A kind of method of water soluble vitamin in detection blood |
-
2019
- 2019-12-05 CN CN201911234804.7A patent/CN110927277A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120048003A1 (en) * | 2010-08-24 | 2012-03-01 | GOLDEN PRODUCTS LLC, Limited Liability Company | Vitamin assay methods |
| CN105424854A (en) * | 2015-11-23 | 2016-03-23 | 济南英盛生物技术有限公司 | Method for detecting various water-soluble vitamins in blood sample at same time |
| CN106248773A (en) * | 2016-07-19 | 2016-12-21 | 中国检验检疫科学研究院 | Vitamin B in a kind of quickly mensuration multidimensional sheet1with ascorbic method |
| CN106442838A (en) * | 2016-10-19 | 2017-02-22 | 杭州佰辰医学检验所有限公司 | Isotope dilution ultra-high performance liquid chromatography-mass spectrometry method for measuring vitamin B1 in serum |
| CN110412175A (en) * | 2019-09-05 | 2019-11-05 | 北京和合医学诊断技术股份有限公司 | A kind of method of water soluble vitamin in detection blood |
Non-Patent Citations (3)
| Title |
|---|
| GENG CM 等: "LC-MS/MS for the Determination of Four Water-Soluble Vitamins: Method Development, Validation and Comparison to EC Method", 《CHROMATOGRAPHIA》 * |
| 全思思: "超高效液相色谱/超临界流体色谱—串联质谱联用技术同时测定婴幼儿配方乳粉中多种维生素的研究", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
| 宁霄 等: "超高效液相串联质谱法同时测定保健食品中10种水溶性维生素", 《中国药事》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114527230A (en) * | 2022-02-28 | 2022-05-24 | 上海药明奥测医疗科技有限公司 | Method for detecting concentration of vitamin A and vitamin E in human dry blood spots |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110542735A (en) | method for high-throughput determination of multiple fat-soluble vitamins by ultra-high performance liquid mass spectrometry | |
| CN111024874A (en) | Method for quantitatively detecting catecholamine and metabolites thereof by liquid chromatography-mass spectrometry | |
| CN106855545B (en) | Method for simultaneously detecting fat-soluble vitamins and water-soluble vitamins in feed | |
| CN105424854A (en) | Method for detecting various water-soluble vitamins in blood sample at same time | |
| CN109001329B (en) | High performance liquid chromatography tandem mass spectrometry detection method for 25-hydroxy vitamin D in dried blood tablets | |
| CN104614479A (en) | Detection method of vitamin in food | |
| CN111175405A (en) | Method for simultaneously detecting multiple fat-soluble vitamins in blood sample and application thereof | |
| den Boer et al. | AU–HPLC–ESI–MS/MS–based stable isotope dilution method for the detection and quantitation of methotrexate in plasma | |
| CN110174476A (en) | The detection method of the Liquid Chromatography-Tandem Mass Spectrometry of a variety of liposoluble vitamins in a kind of dried blood spot | |
| CN110455954A (en) | A kind of catecholamine levels in plasma metabolin high performance liquid chromatography tandem mass spectrum detection method | |
| CN112834677A (en) | Method for simultaneously detecting homocysteine and metabolic related substances thereof | |
| CN112557539A (en) | Method for simultaneously determining vitamin A, vitamin E and 25-hydroxyvitamin D in blood plasma | |
| CN110320302A (en) | The quickly method of measurement methotrexate (MTX) blood concentration | |
| CN111830146B (en) | LC-MS/MS high-throughput detection method and kit for 25-hydroxyvitamin D in dried blood slices | |
| CN112782328A (en) | Method and kit for detecting catecholamine and metabolites thereof in urine and application of kit | |
| CN110927277A (en) | Method for rapidly detecting concentrations of vitamin B1 and vitamin C based on liquid chromatography-tandem mass spectrometry technology | |
| CN107153103B (en) | Method for determining contents of various mycotoxins in fresh milk sample | |
| CN116223693B (en) | Method for measuring folic acid and metabolite thereof in erythrocytes by high performance liquid chromatography tandem mass spectrometry | |
| CN113607854A (en) | Method and detection kit for simultaneously detecting multiple vitamins | |
| Abe et al. | Determination of intracellular ascorbic acid using tandem mass spectrometry | |
| CN111175406B (en) | Method for simultaneously detecting multiple water-soluble vitamins in blood sample and application thereof | |
| CN113075305A (en) | Method for quantitatively detecting content of lipid-soluble vitamins in peripheral blood sample | |
| CN108008035B (en) | Method for detecting purity of 3-ethoxy-4-methoxybenzaldehyde | |
| CN112485340A (en) | Method for detecting 1, 5-sorbitan in plasma by ultra-high performance liquid chromatography tandem mass spectrometry | |
| CN102435699B (en) | Method for rapidly determining melamine in milk and dairy products by liquid chromatography-tandem mass spectrometry |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200327 |
|
| RJ01 | Rejection of invention patent application after publication |