CN110917120A - Sticky antibacterial repairing material and preparation method and application thereof - Google Patents
Sticky antibacterial repairing material and preparation method and application thereof Download PDFInfo
- Publication number
- CN110917120A CN110917120A CN201911163261.4A CN201911163261A CN110917120A CN 110917120 A CN110917120 A CN 110917120A CN 201911163261 A CN201911163261 A CN 201911163261A CN 110917120 A CN110917120 A CN 110917120A
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- Prior art keywords
- antibacterial
- sticky
- bioactive glass
- antimicrobial
- uniformly mixing
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Abstract
The invention discloses a sticky antibacterial repairing material and a preparation method and application thereof, and the sticky antibacterial repairing material comprises, by mass, 0.15-0.2% of mussel mucin, 0.2-2% of D-panthenol, 0.5-10% of modified chitosan, 0.5-10% of bioactive glass, 0.5-10% of a forming agent, 20-50% of a humectant, 0.3-1% of a preservative, 1-5% of a protein protective agent, 0.5-5% of a pH regulator, 13-30% of a functional assistant and the balance of purified water. The preparation method comprises the step of mixing the raw materials in sequence under aseptic condition. The adhesive antibacterial repairing material is directly applied to an affected part in a natural cavity with secretion on a human body or an animal body so as to realize the effects of resisting bacteria, repairing and promoting healing. The invention not only can realize the antibacterial repair function to achieve the aim of healing, but also is convenient for patients and medical care personnel to use, has little or no smell, is not easy to be wiped off, and is easier to get the favor of the patients.
Description
Technical Field
The invention belongs to the technical field of biological antibacterial repair materials, and particularly relates to a sticky antibacterial repair material and a preparation method and application thereof.
Background
The antibacterial repair material has attracted attention as a material which can realize surface antibacterial and wound healing and repair functions. Various products such as antibacterial repair liquid, antibacterial repair gel, antibacterial repair paste and the like are full of eyes in the market. However, although these antibacterial repairing products can achieve the antibacterial repairing effect to a certain extent, the disadvantages of inconvenience in administration, easy scraping or strong odor often occur in the using process of patients, and meanwhile, due to the pharmacological action of the antibacterial components of some antibacterial repairing materials, the bacteria have drug resistance, so that the use of these products is limited.
The antibacterial repairing gynecological gel prepared by using the chitosan iodine solution and the bioactive glass provided by the patent No. CN201410108988.3, the antibacterial repairing functional dressing formed by crosslinking and compounding chitosan and collagen on a bacterial cellulose membrane through a crosslinking agent provided by the patent No. CN201510856221.3 and the iodine-containing cervical antibacterial repairing material prepared by gelatin, water-soluble chitosan, iodine particles and acidic aqueous solution provided by the patent No. CN201010594303.2 can achieve the antibacterial repairing effect. However, these antibacterial repairing materials are difficult to be adhered to the affected part, so as to realize continuous antibacterial repairing, and meanwhile, the phenomenon of medicine abrasion is inevitably generated, so that inconvenience is brought to the patient.
Disclosure of Invention
The invention aims to overcome the problems in the prior art, and provides an antibacterial repairing material with a sticky effect, which not only can realize the antibacterial repairing function to achieve the purpose of healing, but also is convenient for patients and medical care personnel to use, has little or no odor, is not easy to wipe off, and is easier to get the favor of patients.
The invention provides a sticky antibacterial repairing material which comprises, by mass, 0.15-0.2% of mussel mucin, 0.2-2% of D-panthenol, 0.5-10% of modified chitosan, 0.5-10% of bioactive glass, 0.5-10% of a forming agent, 20-50% of a humectant, 0.3-1% of a preservative, 1-5% of a protein protective agent, 0.5-5% of a pH regulator, 13-30% of a functional additive and the balance of purified water.
According to one embodiment of the tacky antibacterial repairing material, the pH value of the tacky antibacterial repairing material is 3.5-5.5.
According to an embodiment of the mucoadhesive antibacterial repair material of the invention, the mussel mucin is present in a liquid form at a concentration of 0.01-300 mg/mL.
According to one embodiment of the tacky antimicrobial repair material, the modified chitosan is carboxymethyl chitosan and/or chitosan quaternary ammonium salt.
According to one embodiment of the sticky antibacterial repairing material, the bioactive glass is 45s bioactive glass powder with the particle size of 20-38 mu m, and SiO is weighed according to mass percentage during preparation 240～58％、P2O54～6％、Na2And uniformly mixing 20-25% of O and 20-35% of CaO, melting at high temperature, cooling, crushing and sieving to obtain bioactive glass powder with a predetermined particle size.
According to one embodiment of the tacky antibacterial repairing material, the forming agent is one or more of polyvinyl alcohol, carbomer, sodium alginate, gelatin and xanthan gum, and the humectant is one or more of glycerin, sorbitol and dodecaneoyl.
According to one embodiment of the mucoadhesive antibacterial repair material, the preservative is dehydroacetic acid and/or methyl paraben, and the protein protectant is human blood albumin.
According to one embodiment of the adhesive antibacterial repairing material, the pH regulator is one or more of citric acid, acetic acid and ethyl acetate, and the functional auxiliary agent is one or more of a trace element agent, a urea capsule and hyaluronic acid.
In another aspect of the present invention, a preparation method of a sticky antibacterial repairing material is provided, which comprises the following steps:
A. under the aseptic condition, uniformly mixing purified water, a humectant and D-panthenol, adding modified chitosan and a forming agent, uniformly mixing, adding bioactive glass, a preservative and a functional assistant, uniformly mixing to obtain a mixed solution, adjusting the pH value of the mixed solution to 3.5-5.5 by using a pH regulator, sterilizing at 120-125 ℃, and cooling for later use; or under the aseptic condition, adding a forming agent into purified water, heating, mixing and dissolving, cooling, adding a humectant and D-panthenol, uniformly mixing, adding modified chitosan, uniformly mixing, adding bioactive glass, a preservative and a functional assistant, uniformly mixing to obtain a mixed solution, adjusting the pH value of the mixed solution to 3.5-5.5 by using a pH regulator, sterilizing at 120-125 ℃, and cooling for later use;
B. under the aseptic condition, adding a protein protective agent into mussel mucin, diluting the mussel mucin to 1-2% of the concentration of the raw material by using purified water, and filtering and sterilizing by using a filter membrane of 0.2-0.5 mu m to obtain a mussel mucin solution for later use;
C. under the aseptic condition, uniformly mixing the substance obtained in the step A and the mussel mucoprotein solution obtained in the step B to obtain the sticky antibacterial repairing material; or carrying out circulating freezing and thawing subsequent treatment to obtain the sticky antibacterial repairing material.
Wherein the adding amount of each raw material is as follows by mass percent: 0.15-0.2% of mussel adhesive protein, 0.2-2% of D-panthenol, 0.5-10% of modified chitosan, 0.5-10% of bioactive glass, 0.5-10% of a forming agent, 20-50% of a humectant, 0.3-1% of a preservative, 1-5% of a protein protective agent, 0.5-5% of a pH regulator, 13-30% of a functional additive and the balance of purified water.
The invention also provides the sticky antibacterial repairing material prepared by the preparation method of the sticky antibacterial repairing material.
In a further aspect, the invention provides an application of the above adhesive antibacterial repairing material, and the adhesive antibacterial repairing material is directly applied to an affected part in a natural cavity with secretion on a human body or an animal body to realize the effects of resisting bacteria, repairing and promoting healing.
The sticky antibacterial repairing material provided by the invention not only can realize the antibacterial action of an affected part, but also can be stuck to the affected part for a long time, so that the continuous antibacterial action of the affected part is realized, and the healing of the affected part is accelerated; moreover, the sticky antibacterial repairing material is not easy to wipe off in the using process, is convenient for patients to use, has the characteristics of no toxic or side effect and small irritation, and has good histocompatibility.
Drawings
Fig. 1 shows a graph of the change in the activity of mussel mucin and pure mussel mucin over time in each example of the experimental examples.
Detailed Description
All of the features disclosed in this specification, or all of the steps in any method or process so disclosed, may be combined in any combination, except combinations of features and/or steps that are mutually exclusive.
Any feature disclosed in this specification may be replaced by alternative features serving equivalent or similar purposes, unless expressly stated otherwise. That is, unless expressly stated otherwise, each feature is only an example of a generic series of equivalent or similar features.
According to an exemplary embodiment of the invention, the sticky and sticky antibacterial repairing material comprises, by mass, 0.15-0.2% of mussel adhesive protein, 0.2-2% of D-panthenol, 0.5-10% of modified chitosan, 0.5-10% of bioactive glass, 0.5-10% of a forming agent, 20-50% of a humectant, 0.3-1% of a preservative, 1-5% of a protein protective agent, 0.5-5% of a pH regulator, 13-30% of a functional additive and the balance of purified water.
The mussel mucoprotein, the D-panthenol, the modified chitosan and the bioactive glass are main functional components of the invention, and the forming agent, the humectant, the preservative, the protein protective agent, the pH regulator and the functional additive are auxiliary components of the invention.
Mussel mucin can form a water-proof adhesion microscopic biological scaffold with positive charges, and the structure of the mussel mucin mainly has three characteristics: 1) wherein, after the dopa is oxidized into the o-diquinone, the dopa can be firmly adhered to the surface of a human body; 2) in the presence of physiological solution, part of hydrophobic genes of the mussel mucin are exposed and can be mutually combined with lipid bilayers of cell membranes through hydrophobic interaction to strengthen the water-proof adhesion of cells; 3) the existence of lysine enables protein to have high-load positive charges, and human patient cells have negative charges, so that the adherence of epidermal cells is promoted, and the healing of a wound surface or an ulcer surface is promoted. Meanwhile, the mussel mucin can be combined with nerve ending cells through electrostatic action, wherein dopa is combined with dopa receptors on the cell surface to block partial signal transmission of nerve cells, so that the effect of relieving itching is achieved.
The modified chitosan as the derivative of nontoxic cationic chitosan can generate electrostatic interaction with bacteria with negative charges on the surface, or be adsorbed on the surface of the bacteria to cause the metabolism to be disordered so as to achieve the bactericidal effect. The modified chitosan has wide antibacterial spectrum, has good antibacterial effect on staphylococcus aureus, candida albicans, escherichia coli and the like, and is not easy to generate drug-resistant strains.
D-panthenol is a precursor of vitamin B5, can deeply penetrate into stratum corneum, has strong moisturizing effect, and can stimulate growth of epithelial cells, promote wound healing, shorten healing time of epidermal wound, repair tissue wound, relieve skin discomfort and pruritus, and stimulate growth of epidermal cells.
Bioactive glass, also known as calcium sodium phosphosilicate, has SiO as main component2、CaO、Na2O and P2O5And the like, which are materials capable of repairing, replacing and regenerating body tissues. Can rapidly react in an aqueous solution environment to release calcium ions, phosphate ions and the like and form a local high-pH environment, thereby inhibiting the growth of various pathogenic bacteria on the affected part and contacting the affected part for a long time to achieve continuous antibiosis and wound repairThe present invention achieves the desired antimicrobial repair effect by selectively adding bioactive glass.
At present, no case of adding bioactive glass, D-panthenol, chitosan quaternary ammonium salt and mussel mucin into the antibacterial repair material is found. The invention organically combines the four functional components together, obtains the antibacterial repair material which can be adhered to a patient for a long time to realize the effects of continuous antibiosis, repair and healing promotion by adjusting the proportion, selecting the auxiliary agent and improving the adaptability of the preparation process, is convenient and quick to use, has no other toxic or side effect and has obvious synergistic effect.
Specifically, the mussel mucin used in the invention exists in a liquid form with the concentration of 0.01-300 mg/mL.
The modified chitosan in the invention can be carboxymethyl chitosan and/or chitosan quaternary ammonium salt, has excellent spectrum antibacterial capability, and is not easy to generate drug-resistant strains. The bioactive glass is preferably 45s bioactive glass powder with the particle size of 20-38 mu m, and SiO is weighed according to mass percentage during preparation 240～58％、P2O54～6％、Na220-25% of O and 20-35% of CaO, uniformly mixing, melting at high temperature, cooling, crushing and sieving to obtain bioactive glass powder with a predetermined particle size, wherein the product of the bioactive glass powder after reaction with water can promote the generation of cell growth factors, improve the activity of cells and promote the proliferation and proliferation of cells. In the case of D-panthenol, it is represented as a deeply penetrating moisturizer in skin care, and it is capable of soothing the skin and relieving the red swelling and stinging symptom of the skin, and it is also an activator of skin regeneration.
In addition, each functional auxiliary agent in the invention can be selected and prepared according to different action positions. Wherein the forming agent can be one or more of polyvinyl alcohol, carbomer, sodium alginate, gelatin and xanthan gum; the preservative can be dehydroacetic acid and/or methyl paraben, and the protein protective agent is preferably human serum albumin; the humectant may be one or more of glycerin, sorbitol, and dodecaneoyl.
The pH regulator can be one or more of citric acid, acetic acid and ethyl acetate, and the functional auxiliary agent can be one or more of microelement agent, urea capsule and hyaluronic acid.
The invention also provides a preparation method of the sticky antibacterial repairing material, which comprises the following steps.
Step A:
under the aseptic condition, uniformly mixing purified water, a humectant and D-panthenol, adding modified chitosan and a forming agent, uniformly mixing, adding bioactive glass, a preservative and a functional assistant, uniformly mixing to obtain a mixed solution, adjusting the pH value of the mixed solution to 3.5-5.5 by using a pH regulator, sterilizing at 120-125 ℃, and cooling for later use; or under the aseptic condition, adding a forming agent into purified water, heating, mixing and dissolving, cooling, adding a humectant and D-panthenol, uniformly mixing, adding modified chitosan, uniformly mixing, adding bioactive glass, a preservative and a functional assistant, uniformly mixing to obtain a mixed solution, adjusting the pH value of the mixed solution to 3.5-5.5 by using a pH regulator, sterilizing at 120-125 ℃, and cooling for later use.
Among them, all the instruments are washed in advance and sterilized.
And B:
under the aseptic condition, adding 1-5% of protein protective agent into mussel mucin, diluting the mussel mucin to 1-2% of the concentration of the raw material by using purified water, and filtering and sterilizing by using a filter membrane of 0.2-0.5 mu m to obtain a mussel mucin solution for later use.
And C:
under the aseptic condition, uniformly mixing the substance obtained in the step A and the mussel mucoprotein solution obtained in the step B to obtain the sticky antibacterial repairing material; or, the uniformly mixed substances are placed in an environment with the temperature of-15 to-20 ℃ for 3 to 5 times of circulating freezing and thawing, and finally the sticky antibacterial repairing material is obtained.
The preparation method comprises the following steps of: 0.15-0.2% of mussel adhesive protein, 0.2-2% of D-panthenol, 1-10% of modified chitosan, 0.5-10% of bioactive glass, 0.5-10% of a forming agent, 20-50% of a humectant, 0.3-1% of a preservative, 1-5% of a protein protective agent, 0.5-5% of a pH regulator, 13-30% of a functional additive and the balance of purified water.
Of course, the sticky antibacterial repairing material prepared by the preparation method of the sticky antibacterial repairing material is also the protection object of the invention.
The sticky antibacterial repairing material provided by the invention has a more advantageous application direction, and particularly can be directly applied to an affected part with secretion in a natural cavity on a human body or an animal body so as to realize the effects of resisting bacteria, repairing and promoting healing. Among them, natural cavities having secretions in the human or animal body include the oral cavity, the liver and intestine, the vagina, and so on.
In order to make the technical means, inventive features, objectives and effects achieved by the present invention easily understandable, the present invention is further described in detail by examples and comparative examples below.
Example 1:
the sticky antibacterial repairing film for treating oral ulcer is prepared according to the following mass percentages: mussel mucin 0.18%, D-panthenol 0.2%, modified chitosan 10%, bioactive glass 0.5%, forming agent 10%, humectant 30%, preservative 0.5%, protein protectant 4%, pH regulator 5%, functional assistant 13% and purified water in balance.
The forming agent is polyvinyl alcohol, the humectant is glycerol and dodecyl neopentyl, the preservative is dehydroacetic acid, the protein protective agent is human blood albumin, the pH regulator is ethyl acetate, the modified chitosan is chitosan quaternary ammonium salt, and the functional auxiliary agent is a mixture of trace element solution including zinc, iron, selenium, copper and the like, hyaluronic acid and urea capsules.
The preparation of this example comprises the following steps:
(1) sterilizing all appliances;
(2) dissolving polyvinyl alcohol in purified water, cooling to room temperature after 2 hours in a water bath at 95 ℃, adding glycerol, dodecyl neopentyl and D-panthenol, continuing stirring, then adding chitosan quaternary ammonium salt, bioactive glass and a functional assistant, uniformly stirring, adding dehydroacetic acid, adjusting the pH value of the mixed solution to 3.5-4.0 by using ethyl acetate, sterilizing at 120 ℃ under high pressure, and cooling the mixed solution for later use;
(3) measuring a certain amount of mussel mucin raw material liquid, adding 4% of protein protective agent, diluting with purified water to 1% of the raw material concentration, and filtering and sterilizing by using a 0.2 μm filter membrane under aseptic condition to obtain mussel mucin solution for later use;
(4) and (3) under the aseptic condition, uniformly mixing the mixed solution in the step (2) with the mussel mucin solution in the step (3), then placing the mixture in an environment with the temperature of between 15 ℃ below zero and 20 ℃ below zero, freezing and unfreezing for 3 times, and finally obtaining the sticky antibacterial repairing material for treating the oral ulcer.
Example 2:
the sticky antibacterial repairing film for treating oral ulcer is prepared according to the following mass percentages: mussel mucin 0.16%, D-panthenol 2%, modified chitosan 5%, bioactive glass 5%, forming agent 6%, humectant 25%, preservative 0.7%, protein protective agent 3%, pH regulator 2.6%, functional assistant 20% and purified water in balance.
The forming agent is polyvinyl alcohol, the humectant is sorbitol and dodecyl neopentyl, the preservative is dehydroacetic acid, the protein protective agent is human blood albumin, the pH regulator is acetic acid, the modified chitosan is carboxymethyl chitosan, and the functional auxiliary agent is a mixture of trace element solution including zinc, iron, selenium, copper and the like, hyaluronic acid and urea capsules.
The preparation of this example comprises the following steps:
(1) sterilizing all appliances;
(2) dissolving polyvinyl alcohol in purified water, cooling to room temperature after 2 hours in a water bath at 95 ℃, adding dodecaneoyl and D-panthenol, continuing stirring, then adding carboxymethyl chitosan, bioactive glass and a functional assistant, uniformly stirring, adding dehydroacetic acid, then adjusting the pH value of the mixed solution to 3.5-4.0 by using acetic acid, sterilizing at the high pressure of 125 ℃, and cooling the mixed solution for later use;
(3) measuring a certain amount of mussel mucin raw material liquid, adding 3% of protein protective agent, diluting with purified water to 1.5% of the raw material concentration, and filtering and sterilizing by using a 0.25 mu m filter membrane under an aseptic condition to obtain mussel mucin solution for later use;
(4) and (3) under the aseptic condition, uniformly mixing the mixed solution in the step (2) with the mussel mucin solution in the step (3), and then placing the mixture in an environment with the temperature of between 15 ℃ below zero and 20 ℃ below zero for freezing and thawing for 4 times, thereby finally obtaining the sticky antibacterial repairing material for treating the oral ulcer.
Example 3:
the sticky antibacterial repairing film for treating oral ulcer is prepared according to the following mass percentages: mussel mucin 0.2%, D-panthenol 2%, modified chitosan 1%, bioactive glass 10%, forming agent 8%, humectant 20%, preservative 0.3%, protein protective agent 5%, pH regulator 0.5%, functional assistant 26% and purified water in balance.
The forming agent is polyvinyl alcohol, the humectant is glycerin, the preservative is methyl paraben, the protein protective agent is human blood albumin, the pH regulator is citric acid, the modified chitosan is chitosan quaternary ammonium salt, and the functional auxiliary agent is a mixture of trace element solution including zinc, iron, selenium, copper and the like, hyaluronic acid and urea capsules.
The preparation of this example comprises the following steps:
(1) sterilizing all appliances;
(2) dissolving polyvinyl alcohol in purified water, cooling to room temperature after 2 hours in a water bath at 95 ℃, adding glycerol and D-panthenol, continuously stirring, then adding chitosan quaternary ammonium salt, bioactive glass and a functional assistant, uniformly stirring, adding methyl paraben, adjusting the pH value of the mixed solution to 3.5-5.0 by using citric acid, sterilizing at the high pressure of 125 ℃, and cooling the mixed solution for later use;
(3) measuring a certain amount of mussel mucin raw material liquid, adding a 5% protein protective agent, diluting with purified water to 2% of the raw material concentration, and filtering and sterilizing by using a 0.3 mu m filter membrane under an aseptic condition to obtain a mussel mucin solution for later use;
(4) and (3) under the aseptic condition, uniformly mixing the mixed solution in the step (2) with the mussel mucin solution in the step (3), and then placing the mixture in an environment with the temperature of between 15 ℃ below zero and 20 ℃ below zero for 5 times of freezing and thawing cycles to finally obtain the sticky antibacterial repairing material for treating the oral ulcer.
Example 4:
the sticky antibacterial repairing gel for anorectal parts is prepared according to the following mass percentages: mussel mucin 0.2%, D-panthenol 1%, modified chitosan 5%, bioactive glass 8%, forming agent 3%, humectant 35%, preservative 1%, protein protectant 4.5%, pH regulator 0.5%, functional assistant 20% and purified water in balance.
The forming agent is carbomer, the humectant is lauryl neoyl and glycerol, the pH regulator is acetic acid, the preservative is dehydroacetic acid, the modified chitosan is carboxymethyl chitosan, and the functional auxiliary agent is a mixture of trace element solution including zinc, iron, selenium, copper and the like, medium molecular weight hyaluronic acid, small molecular weight hyaluronic acid and urea capsules.
The preparation of this example comprises the following steps:
(1) sterilizing all appliances;
(2) dissolving carbomer in a mixed solution of purified water, dodecaneoyl and glycerol, adding D-panthenol after uniformly stirring, continuously stirring, then adding carboxymethyl chitosan, dehydroacetic acid, bioactive glass and a functional assistant, uniformly stirring, then adjusting the pH value of the mixed solution to 3.8-4 by using acetic acid, sterilizing at 120 ℃ under high pressure, and cooling the mixed solution for later use;
(3) measuring a certain amount of mussel adhesive protein raw material liquid, adding 4.5% of protein protective agent, diluting with purified water to 1.5% of the raw material concentration, and filtering and sterilizing with a 0.45 μm filter membrane under aseptic condition to obtain mussel adhesive protein solution for later use;
(4) and (3) under the aseptic condition, uniformly mixing the mixed solution in the step (2) with the mussel mucoprotein solution in the step (3) to obtain the sticky antibacterial repairing material.
Example 5:
the sticky antibacterial repairing gel for anorectal parts is prepared according to the following mass percentages: mussel mucin 0.18%, D-panthenol 1.6%, modified chitosan 10%, bioactive glass 8%, forming agent 1%, humectant 25%, preservative 0.6%, protein protectant 3.5%, pH regulator 2.5%, functional assistant 30% and purified water in balance.
The forming agent is xanthan gum, the humectant is dodecyl neopentyl, the pH regulator is ethyl acetate, the preservative is methyl paraben, the modified chitosan is chitosan quaternary ammonium salt, and the functional auxiliary agent is a mixture of trace element solution including zinc, iron, selenium, copper and the like, medium molecular weight hyaluronic acid, small molecular weight hyaluronic acid and urea capsules.
The preparation of this example comprises the following steps:
(1) sterilizing all appliances;
(2) dissolving xanthan gum in a mixed solution of purified water and dodecyl neopentyl glycol, adding D-panthenol after uniformly stirring, continuously stirring, then adding chitosan quaternary ammonium salt, methyl paraben, bioactive glass and a functional assistant, uniformly stirring, then adjusting the pH value of the mixed solution to 3.5-4.5 by using ethyl acetate, sterilizing at 120 ℃ under a high pressure condition, and cooling the mixed solution for later use;
(3) measuring a certain amount of mussel mucin raw material liquid, adding 3.5% of protein protective agent, diluting with purified water to 2% of the raw material concentration, and filtering and sterilizing by using a 0.3 mu m filter membrane under an aseptic condition to obtain mussel mucin solution for later use;
(4) and (3) under the aseptic condition, uniformly mixing the mixed solution in the step (2) with the mussel mucoprotein solution in the step (3) to obtain the sticky antibacterial repairing material.
Example 6:
the sticky antibacterial repairing gel for anorectal parts is prepared according to the following mass percentages: mussel mucin 0.16%, D-panthenol 2%, modified chitosan 6%, bioactive glass 3%, forming agent 5%, humectant 22%, preservative 0.8%, protein protective agent 1%, pH regulator 5%, functional assistant 25% and purified water in balance.
The forming agent is gelatin, the humectant is glycerol and sorbitol, the pH regulator is ethyl acetate, the preservative is dehydroacetic acid, the modified chitosan is chitosan quaternary ammonium salt, and the functional auxiliary agent is a mixture of trace element solution including zinc, iron, selenium, copper and the like, medium molecular weight hyaluronic acid, small molecular weight hyaluronic acid and urea capsules.
The preparation of this example comprises the following steps:
(1) sterilizing all appliances;
(2) dissolving gelatin in a mixed solution of purified water, glycerol and sorbitol, adding D-panthenol after uniformly stirring, continuously stirring, then adding chitosan quaternary ammonium salt, dehydroacetic acid, bioactive glass and a functional assistant, uniformly stirring, then adjusting the pH value of the mixed solution to 4-5 by using the dehydroacetic acid, sterilizing at 125 ℃ under a high pressure condition, and cooling the mixed solution for later use;
(3) measuring a certain amount of mussel mucin raw material liquid, adding 1% of protein protective agent, diluting with purified water to 1.4% of the raw material concentration, and filtering and sterilizing by using a 0.5-micron filter membrane under aseptic condition to obtain mussel mucin solution for later use;
(4) and (3) under the aseptic condition, uniformly mixing the mixed solution in the step (2) with the mussel mucoprotein solution in the step (3) to obtain the sticky antibacterial repairing material.
Example 7:
in this example, the sticky antibacterial repairing liquid for the female vaginal part is prepared according to the following mass percentages: mussel mucin 0.15%, D-panthenol 2.0%, modified chitosan 8%, bioactive glass 5%, forming agent 0.5%, humectant 33%, preservative 0.3%, protein protectant 2%, pH regulator 3%, functional assistant 13% and purified water in balance.
The forming agent is sodium alginate, the humectant is a mixed solution of dodecyl neopentyl, sorbitol and glycerol, the pH regulator is citric acid, the preservative is methyl paraben, the modified chitosan is chitosan quaternary ammonium salt, and the functional additive is a mixture of medium molecular weight hyaluronic acid, small molecular weight hyaluronic acid and urea capsules.
The preparation of this example comprises the following steps:
(1) sterilizing all appliances;
(2) dissolving sodium alginate in a proper amount of purified water and a mixed solution of dodecyl neopentyl, sorbitol and glycerol, uniformly stirring, adding D-panthenol, continuously stirring, adding chitosan quaternary ammonium salt, bioactive glass and a functional assistant, uniformly stirring, adding methyl hydroxybenzoate, continuously stirring, adjusting the pH value of the mixed solution to 3.8-4 by using citric acid after the sodium alginate is dissolved, sterilizing at 120 ℃ under high pressure, and cooling the mixed solution for later use;
(3) measuring a certain amount of mussel adhesive protein raw material liquid, adding 2% of protein protective agent, diluting with purified water to 2% of the raw material concentration, and filtering and sterilizing with a 0.3 μm filter membrane under aseptic condition to obtain mussel adhesive protein liquid for later use;
(4) and (3) under the aseptic condition, uniformly mixing the mixed solution in the step (2) with the mussel mucoprotein solution in the step (3) to obtain the sticky antibacterial repairing material.
Example 8:
in this example, the sticky antibacterial repairing liquid for the female vaginal part is prepared according to the following mass percentages: mussel mucin 0.17%, D-panthenol 1.5%, modified chitosan 9%, bioactive glass 3%, forming agent 0.5%, humectant 50%, preservative 0.2%, protein protectant 3%, pH regulator 3%, functional assistant 18% and purified water in balance.
The forming agent is polyvinyl alcohol, the humectant is a mixed solution of dodecyl neopentyl and sorbitol, the pH regulator is ethyl acetate, the preservative is dehydroacetic acid, the modified chitosan is carboxymethyl chitosan, and the functional auxiliary agent is a mixture of medium molecular weight hyaluronic acid, small molecular weight hyaluronic acid and urea capsules.
The preparation of this example comprises the following steps:
(1) sterilizing all appliances;
(2) adding polyvinyl alcohol into a proper amount of purified water, heating to 95 ℃, stirring, cooling to room temperature after the polyvinyl alcohol is completely dissolved, adding the polyvinyl alcohol into a mixed solution of dodecyl neopentyl and sorbitol, adding D-panthenol after stirring uniformly, continuing stirring, then adding carboxymethyl chitosan, bioactive glass and a functional assistant, continuing stirring, adding dehydroacetic acid, continuing stirring, adjusting the pH value of the mixed solution to 3.5-4 by using ethyl acetate after the polyvinyl alcohol is dissolved, sterilizing at 125 ℃ under high pressure, and cooling the mixed solution for later use;
(3) measuring a certain amount of mussel adhesive protein raw material liquid, adding 3% of protein protective agent, diluting with purified water to 1.6% of the raw material concentration, and filtering and sterilizing with a 0.2 μm filter membrane under aseptic condition to obtain mussel adhesive protein liquid for later use;
(4) and (3) under the aseptic condition, uniformly mixing the mixed solution in the step (2) with the mussel mucoprotein solution in the step (3) to obtain the sticky antibacterial repairing material.
Example 9:
in this example, the sticky antibacterial repairing liquid for the female vaginal part is prepared according to the following mass percentages: mussel mucin 0.19%, D-panthenol 1.1%, modified chitosan 7%, bioactive glass 6%, forming agent 0.7%, humectant 32%, preservative 0.5%, protein protectant 4%, pH regulator 3%, functional assistant 25% and purified water in balance.
The forming agent is xanthan gum, the humectant is a mixed solution of sorbitol and glycerol, the pH regulator is acetic acid, the preservative is methyl paraben, the modified chitosan is chitosan quaternary ammonium salt, and the functional auxiliary agent is a mixture of medium molecular weight hyaluronic acid, small molecular weight hyaluronic acid and urea capsules.
The preparation of this example comprises the following steps:
(1) sterilizing all appliances;
(2) dissolving xanthan gum in a mixed solution of purified water, glycerol and sorbitol, adding D-panthenol after uniformly stirring, continuously stirring, then adding chitosan quaternary ammonium salt, methyl paraben, bioactive glass and a functional assistant, uniformly stirring, then adjusting the pH value of the mixed solution to 4-5 by using acetic acid, sterilizing at 125 ℃ under a high pressure condition, and cooling the mixed solution for later use;
(3) measuring a certain amount of mussel mucin raw material liquid, adding 4% of protein protective agent, diluting with purified water to 1.4% of the raw material concentration, and filtering and sterilizing by using a 0.25 mu m filter membrane under an aseptic condition to obtain mussel mucin solution for later use;
(4) and (3) under the aseptic condition, uniformly mixing the mixed solution in the step (2) with the mussel mucoprotein solution in the step (3) to obtain the sticky antibacterial repairing material.
Comparative example 1: essentially the same procedure as in example 1 is followed, except for the mussel mucin and protein protectant and the corresponding addition step.
Comparative example 2: essentially the same procedure as in example 1 was followed, except for the addition of the bioactive glass, D-panthenol and modified chitosan and the corresponding addition steps.
Comparative example 3: essentially the same procedure as in example 1 is followed, except that mussel mucin and protein protectant, bioactive glass, D-panthenol and modified chitosan and the corresponding addition steps.
Comparative example 4: essentially the same procedure as in example 4 is followed, except for the mussel mucin and protein protectant and the corresponding addition step.
Comparative example 5: essentially the same procedure as in example 4 was followed, except for the addition of the bioactive glass, D-panthenol and modified chitosan and the corresponding addition steps.
Comparative example 6: essentially the same procedure as in example 4 is followed, except for the mussel mucin and protein protectant, bioactive glass, D-panthenol and modified chitosan and the corresponding addition steps.
Comparative example 7: essentially the same procedure as in example 7 is followed, except for the mussel mucin and protein protectant and the corresponding addition step.
Comparative example 8: essentially the same procedure as in example 7 was followed, except for the addition of the bioactive glass, D-panthenol and modified chitosan and the corresponding addition steps.
Comparative example 9: essentially the same procedure as in example 7 is followed, except that mussel mucin and protein protectant, bioactive glass, D-panthenol and modified chitosan and the corresponding addition steps.
A plurality of tests were carried out below with respect to each of the above examples and comparative examples.
Experimental example 1: stability test
In order to verify the stability performance of the product, the product is subjected to stability experimental verification. The materials of the examples were packaged according to the operating protocol, and were placed at a low temperature of 5 ℃ and a normal temperature of 20 ℃ for 12 months, respectively, to be sampled, and the performance indexes thereof were measured as shown in table 1 below.
Table 1 stability test results for various examples under different storage conditions
From the results shown in Table 1, it is understood that the tacky antibacterial restorative materials provided in examples 1, 4 and 7 were all free from the appearance of mildew, discoloration and odor when stored at 5 ℃ and 20 ℃ for 1 to 12 months, and in example 7, the mixture had insoluble substances, so that a part of the material was precipitated, and the material was shaken up and down several times during use. The biological activity of the mytilus edulis mucin of the sticky antibacterial repairing material is poor in stability at the room temperature of 20 ℃, the activity is obviously reduced after the material is placed for 3 months, and the biological activity is not obviously changed at the temperature of 5 ℃. Examples 2 to 3, examples 5 to 6 and examples 8 to 9 also have similar test results to those described above, in addition to example 1, example 4 and example 7.
Experimental example 2: tack time test for tacky materials
The examples 1 to 9 and comparative examples 1, 4 and 7 were subjected to tack time comparison tests, the results of which are shown in Table 2. Meanwhile, the examples and pure mussel mucin were subjected to a mussel mucin bioactivity test, and the results are shown in fig. 1 with pure mussel mucin as a blank group.
TABLE 2 tack time and Mytilus galloprovincialis mucin bioactivity test results for examples 1-9 and comparative examples 1, 4, 7
As shown in table 2, the sticky antibacterial repairing material provided by the embodiment of the present invention has a longer sticky time than the antibacterial repairing material in the comparative example, so that the antibacterial repairing material applied to the affected part has an increased time for repairing the affected part, which is beneficial to healing the affected part. From FIG. 1, it can be seen that mussel mucin has still good biological activity in the substrates of examples 1-9.
Example 3: antibacterial test
Sample preparation: inoculating bacteria (Escherichia coli) into sterilized broth culture medium, culturing at 35 deg.C overnight, and making into bacterial suspension; diluting the original bacterial suspension (mother liquor) to 106cfu/ml was used as a bacterial solution for the experiment.
The samples of examples 1 to 9 and comparative examples 1 to 9 were inoculated into the above-mentioned bacterial solution, and the bacteriostatic effect was measured using sterile water as a blank, and the results are shown in table 3 below.
TABLE 3 antibacterial Effect of examples 1 to 9 and comparative examples 1 to 9
As can be seen from Table 3, examples 1-9, comparative example 1, comparative example 4 and comparative example 7 provided by the present invention all have good bacteriostatic effect, but the bacteriostatic effect of the example products obtained after adding the mussel mucin is better. Comparing examples 1-9 with comparative examples 1-9, it can be seen that: the antibacterial property of the material is greatly improved by adding the bioactive glass, the D-panthenol and the modified chitosan, and the material is an antibacterial main raw material of the material.
Experimental example 4: skin irritation test
In the guinea pig skin irritation experiment, after the skin is respectively smeared on a depilated area once or for multiple times by using the examples 1-9 provided by the invention, the skin is observed to have no red, rash, blister and other phenomena, and the result shows that the sticky repairing material provided by the invention has no irritation reaction.
Experimental example 5: skin allergy test
In a guinea pig skin irritation experiment, the examples 1 to 9 of the present invention were applied to a depilatory area three times at intervals of 1 week, and 14 days after the last sensitization, the examples were applied to an excitation area, and no redness, rash, blisters, etc. were observed, and the results showed that the adhesive repair material of the present invention had no allergic reaction.
Experimental example 6: clinical trial for treating oral ulcer
1) Case selection: patients were randomized A, B into two groups, and group A was randomized into treatment, control and blank groups (corresponding to example 1, commercially available ordinary canker sore patches and blank groups, respectively), group B was in the same pattern as group A, and the number of patients in each group was 20; the age is 18-55 years, and the average age is 30 years; the course of disease is 1-3 days, and the average course of disease is 2 days.
2) Group a treatment methods: the treatment group is prepared by adopting the material prepared in the embodiment 1, after each meal, cleaning the oral cavity, sticking the ulcer patch to the ulcer part, and lightly pressing for 2-3 s; in the control group, after each meal, cleaning the oral cavity, and selecting a commercially available common oral ulcer patch to be stuck to the ulcer part; in the blank group, after each meal, the oral cavity was cleaned and then rinsed with normal saline, and the therapeutic effects are shown in table 4.
3) Group B treatment methods: the treatment group is prepared by adopting the material prepared in the embodiment 1, and after breakfast and breakfast, cleaning the oral cavity, sticking the ulcer patch to the ulcer part, and lightly pressing for 2-3 s; in the control group, after breakfast and breakfast, cleaning oral cavity, selecting common oral ulcer patch on market to be pasted on ulcer; the blank group, after breakfast only, cleaned the oral cavity and rinsed with normal saline, and the therapeutic effects are shown in table 5.
TABLE 4 clinical observations of oral ulcers
TABLE 5 clinical observations of oral ulcers
As can be seen from tables 4 and 5, the material obtained in example 1 had a better therapeutic effect on canker sores within 36 hours, and was superior to the commercially available canker sore patches. Meanwhile, as can be seen from table 5, since the sticky antibacterial repairing material obtained in example 1 can be permanently stuck to the affected part to realize antibacterial repairing, the reduction of the dressing change times has little influence on the effect of the material of example 1 in treating dental ulcer, but obviously influences the effect of the control group in treating dental ulcer. The materials prepared in examples 2 to 3 were subjected to the same clinical test, and the results of the test and the tendency of change were the same as those of example 1.
Experimental example 7: clinical trial for the treatment of hemorrhoids
The invention carries out clinical effect experiments on the above example 4, and purchases the existing Mayinglong musk hemorrhoid ointment for comparison as a control group. General data: the comparative clinical trial of example 4 above was conducted in comparison with the comparative example, and 300 patients, 150 male and 150 female, aged 18-40 years, were evenly distributed, wherein 300 patients suffered from hemorrhoids for 1 year on average and the longest one was not more than two years, and were randomly divided into A, B two groups of 150 patients each, and then A, B two groups were divided into 50 patients each, a treatment group, a control group and a blank group.
Group a treatment methods: the treatment group, using the material prepared in example 4, was applied to the affected area uniformly in the morning and evening; selecting a commercially available Malilong musk hemorrhoid ointment for uniformly applying to the affected part in the morning and at night; blank group, using normal saline to wash affected part, once in the morning and evening. The condition of the patients after use is continuously observed for 30 days. The treatment results are shown in table 6 below.
Group B treatment methods: the treatment group, using the material prepared in example 4, was applied to the affected part evenly only at night; in the control group, the commercially available Mayinglong musk hemorrhoid ointment is selected and uniformly applied to the affected part only at night; blank group, the affected part was washed with normal saline only once at night. The condition of the patients after use is continuously observed for 30 days. The treatment results are shown in table 7 below.
The curative effect standard is as follows:
1. and (3) healing: the hemorrhoids obviously subside, and the hemorrhoids of the patient have no bleeding and pruritus symptoms;
2. improvement: the hemorrhoids are partially resolved, and the itching of the patient is obviously relieved
3. And (4) invalidation: the hemorrhoids were unchanged.
TABLE 6 clinical observations of hemorrhoids
TABLE 7 clinical observations of hemorrhoids
As can be seen from tables 6 and 7, the material of example 4 of the present invention has a hemorrhoid cure rate of 90% or more, and the therapeutic effect is significantly better than that of the currently available ointment. Meanwhile, as can be seen from table 7, since the tacky antibacterial restorative material obtained in example 4 could be stuck to the affected part, continuous antibacterial restoration was achieved. Therefore, the ointment can be used once a day, and has obvious advantages compared with other commercially available ointments. The materials prepared in examples 5 to 6 were subjected to the same clinical test, and the results of the test and the tendency of change were the same as those of example 4.
Experimental example 8: clinical trial for treating vaginitis
180 vaginitis patients aged 19-58 years were selected and randomized into A, B groups of 90 people each. A, B were then divided into two groups, a treatment group, a control group and a blank group, each group containing 30 persons.
Group a treatment methods: treatment groups, using the material prepared in example 7, were vaginal irrigated, once in the morning and once in the evening; selecting commercially available vaginitis lotion for vaginal irrigation in the morning and evening respectively for a control group; the blank group was washed with water and the vagina was washed once in the morning and once in the evening. The patient was observed for 20 consecutive days of use. The treatment results are shown in table 8 below.
Group B treatment methods: the treatment group, using the material prepared in example 7, was vaginal flushed and used once at night; selecting commercially available vaginitis lotion for vaginal irrigation in a control group, and using the vaginitis lotion once at night; the blank group is washed with clear water for vaginal irrigation and is used once at night. The patient was observed for 20 consecutive days of use. The treatment results are shown in table 9 below.
The curative effect standard is as follows:
1. and (3) healing: leukorrhagia and pruritus vulvae;
2. improvement: the leucorrhea is reduced, and the itching symptom of the patient is obviously relieved
3. And (4) invalidation: the symptoms of vaginitis are unchanged.
TABLE 8 clinical observations of vaginitis
TABLE 9 clinical observations of vaginitis
As can be seen from tables 8 and 9, the antibacterial repair material of the present invention has a cure rate of vaginitis as high as 90% or more, and has obvious advantages over commercially available vaginal lotions. Through the treatment groups of comparison table 8 and table 9, it can be found that the daily administration frequency has little influence on the treatment effect of vaginitis, and the main reason is that the sticky antibacterial repair material obtained in example 7 can be stuck to the affected part and is not easy to drop, so that the affected part can be continuously repaired in an antibacterial manner, and the sticky antibacterial repair material has obvious advantages compared with other commercially available lotion. The materials prepared in examples 8 to 9 were subjected to the same clinical test, and the results of the test and the tendency of change were the same as those of example 7.
The invention is not limited to the foregoing embodiments. The invention extends to any novel feature or any novel combination of features disclosed in this specification and any novel method or process steps or any novel combination of features disclosed.
Claims (10)
1. The sticky antibacterial repairing material is characterized by comprising, by mass, 0.15-0.2% of mussel mucin, 0.2-2% of D-panthenol, 0.5-10% of modified chitosan, 0.5-10% of bioactive glass, 0.5-10% of a forming agent, 20-50% of a humectant, 0.3-1% of a preservative, 1-5% of a protein protective agent, 0.5-5% of a pH regulator, 13-30% of a functional assistant and the balance of purified water.
2. The tacky antimicrobial restorative material of claim 1, wherein the tacky antimicrobial restorative material has a pH of 3.5-5.5.
3. The tacky antimicrobial restorative material of claim 1, wherein the mussel mucin is present in a liquid form at a concentration of 0.01-300 mg/mL.
4. The tacky antimicrobial repair material of claim 1 wherein the modified chitosan is carboxymethyl chitosan and/or chitosan quaternary ammonium salt.
5. The tacky antimicrobial repair material of claim 1 wherein the bioactive glass is 45s bioactive glass with a particle size of 20-38 μmPowder prepared by weighing SiO in mass percent240～58％、P2O54～6％、Na2And uniformly mixing 20-25% of O and 20-35% of CaO, melting at high temperature, cooling, crushing and sieving to obtain bioactive glass powder with a predetermined particle size.
6. The tacky antimicrobial repair material of claim 1 wherein the forming agent is one or more of polyvinyl alcohol, carbomer, sodium alginate, gelatin and xanthan gum, the humectant is one or more of glycerin, sorbitol and lauryl, the preservative is dehydroacetic acid and/or methylparaben, and the protein protectant is human serum albumin.
7. The tacky antimicrobial repair material of claim 1 wherein the pH modifier is one or more of citric acid, acetic acid and ethyl acetate and the functional adjuvant is one or more of a trace element agent, urea vesicle and hyaluronic acid.
8. The preparation method of the sticky antibacterial repairing material is characterized by comprising the following steps of:
A. under the aseptic condition, uniformly mixing purified water, a humectant and D-panthenol, adding modified chitosan and a forming agent, uniformly mixing, adding bioactive glass, a preservative and a functional assistant, uniformly mixing to obtain a mixed solution, adjusting the pH value of the mixed solution to 3.5-5.5 by using a pH regulator, sterilizing at 120-125 ℃, and cooling for later use; or under the aseptic condition, adding a forming agent into purified water, heating, mixing and dissolving, cooling, adding a humectant and D-panthenol, uniformly mixing, adding modified chitosan, uniformly mixing, adding bioactive glass, a preservative and a functional assistant, uniformly mixing to obtain a mixed solution, adjusting the pH value of the mixed solution to 3.5-5.5 by using a pH regulator, sterilizing at 120-125 ℃, and cooling for later use;
B. under the aseptic condition, adding a protein protective agent into mussel mucin, diluting the mussel mucin to 1-2% of the concentration of the raw material by using purified water, and filtering and sterilizing by using a filter membrane of 0.2-0.5 mu m to obtain a mussel mucin solution for later use;
C. under the aseptic condition, uniformly mixing the substance obtained in the step A and the mussel mucoprotein solution obtained in the step B to obtain the sticky antibacterial repairing material; or, the sticky antibacterial repairing material is obtained after the subsequent treatment of circulating freezing and thawing.
Wherein the adding amount of each raw material is as follows by mass percent: 0.15-0.2% of mussel adhesive protein, 0.2-2% of D-panthenol, 0.5-10% of modified chitosan, 0.5-10% of bioactive glass, 0.5-10% of a forming agent, 20-50% of a humectant, 0.3-1% of a preservative, 1-5% of a protein protective agent, 0.5-5% of a pH regulator, 13-30% of a functional additive and the balance of purified water.
9. An adhesive antibacterial restorative material produced by the method for producing an adhesive antibacterial restorative material according to claim 8.
10. Use of the mucoadhesive antimicrobial repair material according to any one of claims 1 to 7 and 9, wherein the mucoadhesive antimicrobial repair material is applied directly to the affected part of the human or animal body in the natural orifice with secretions to achieve the antimicrobial, repair and healing promoting effects.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111973551A (en) * | 2020-08-31 | 2020-11-24 | 西安中天生物医药有限公司 | Mussel mucin antibacterial gel and preparation method thereof |
| CN113975228A (en) * | 2021-10-18 | 2022-01-28 | 江西省肿瘤医院(江西省第二人民医院、江西省癌症中心) | Gynecological antibacterial gel and application thereof |
| CN115887732A (en) * | 2022-12-23 | 2023-04-04 | 稳得希林(沈阳)生物科技有限公司 | Medical gelatin dressing and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104127437A (en) * | 2014-07-24 | 2014-11-05 | 北京大清生物技术有限公司 | Composition with multiple oral treatment and healthcare functions and preparation method thereof |
| CN104645320A (en) * | 2015-01-28 | 2015-05-27 | 南京航空航天大学 | Mussel mucoprotein gel for wound repair, and preparation method and application thereof |
-
2019
- 2019-11-25 CN CN201911163261.4A patent/CN110917120A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104127437A (en) * | 2014-07-24 | 2014-11-05 | 北京大清生物技术有限公司 | Composition with multiple oral treatment and healthcare functions and preparation method thereof |
| CN104645320A (en) * | 2015-01-28 | 2015-05-27 | 南京航空航天大学 | Mussel mucoprotein gel for wound repair, and preparation method and application thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111973551A (en) * | 2020-08-31 | 2020-11-24 | 西安中天生物医药有限公司 | Mussel mucin antibacterial gel and preparation method thereof |
| CN113975228A (en) * | 2021-10-18 | 2022-01-28 | 江西省肿瘤医院(江西省第二人民医院、江西省癌症中心) | Gynecological antibacterial gel and application thereof |
| CN115887732A (en) * | 2022-12-23 | 2023-04-04 | 稳得希林(沈阳)生物科技有限公司 | Medical gelatin dressing and preparation method thereof |
| CN115887732B (en) * | 2022-12-23 | 2024-03-22 | 稳得希林(沈阳)生物科技有限公司 | Medical gelatin dressing and preparation method thereof |
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