CN110882838A - Method for automatically screening active substance - Google Patents
Method for automatically screening active substance Download PDFInfo
- Publication number
- CN110882838A CN110882838A CN201911046964.9A CN201911046964A CN110882838A CN 110882838 A CN110882838 A CN 110882838A CN 201911046964 A CN201911046964 A CN 201911046964A CN 110882838 A CN110882838 A CN 110882838A
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- screening
- stirring rod
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- screened
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
Abstract
The invention provides an active substance automatic screening method, which screens and separates active substances by using an active substance automatic screening device, wherein the screening device is a closed box body, and a horizontally movable sample device and a vertically movable stirring device are arranged in the screening device, and the method comprises the following steps: moving the pipette tip to suck the cleaning solution, and translating the stirring rod to enable the spray port of the stirring rod to be aligned with the sample pool; starting the air suction pump to perform air suction and air discharge actions, so that the magnetic bead emulsion and the substances to be screened are fully and uniformly mixed to perform affinity reaction; moving the magnetic stone to the lower end of the test material pool, opening the electromagnetic valve to enable magnetic bead particles to be adsorbed at the bottom of the test material pool, and fully combining active substances in the substances to be screened with the magnetic beads; the cleaning is repeated for a plurality of times, and the waste liquid is discharged into a waste material tray.
Description
Technical Field
The invention relates to a screening method, in particular to an automatic active substance screening method.
Background
The nano magnetic microspheres are concerned by people in the aspects of molecular biology, medical diagnosis and treatment, bioengineering and environmental pollutant removal. In particular to the diagnosis, extraction, drug delivery and cancer thermotherapy of the nanometer magnetic microsphere in DNA, protein and virus cells; carrying out magnetic SNP detection and mRNA analysis after nucleic acid immobilization; the antibody-immobilized magnetic material provides a novel method for measuring a trace amount of antigen. The target cell (such as cancer cell separation) separated from the pathogen has great application value in the targeted therapy and the evaluation of the effect of the treatment process. It has become one of the most important analytical tools in the life science of the 21 st century and in the field of exploring human mystery and disease treatment.
There have been few patents and reports on methods for automatically screening bioactive substances (proteins). Because the biological active substance protein and the small molecular compound are easy to be inactivated due to the change of the structure of the biological active substance protein and the small molecular compound caused by heating, the temperature keeping is crucial during screening, an automatic device used in the active substance screening method in the current market has a large volume, is provided with a constant temperature tank, a separation tank, a cleaning tank and a waste liquid discharge tank, all the tanks are relatively independent, and the constant temperature is difficult to achieve during the operation of all the links. The purified active substance is impure or inactivated when not used under uniform temperature conditions for a long time. Meanwhile, the thermostatic bath has the defects of large volume, long cooling time and high cost.
Disclosure of Invention
In view of the above, the present invention overcomes the above disadvantages and provides an automated screening method for active substances capable of maintaining the temperature of the active substances constant during screening and separation.
In order to achieve the above object, the present invention provides an active material automatic screening method, which is suitable for screening and separating an active material, wherein the active material automatic screening method utilizes an active material automatic screening device to automatically screen, the screening device is a closed box body, a horizontally movable sample device and a vertically movable stirring device are arranged in the closed box body, the sample device comprises a moving platform and a sample cell, and the moving platform drives the sample cell to horizontally move; the stirring device comprises a stirring rod and a magnet attraction device, wherein the stirring rod drives the magnet attraction device to move up and down, a substance to be screened is put into the sample cell, and the substance to be screened is combined with the nano magnetic beads on the magnet attraction device for screening and separation.
As a further improvement of the invention, the screening device also comprises a heater and a cooler which are arranged at the rear part of the stirring rod and move synchronously with the stirring rod.
As a further improvement of the invention, the screening device also comprises a liquid transfer gun which is arranged at the upper part of the stirring rod, and the liquid transfer gun performs air suction and air discharge through an air suction pump to realize suction and discharge of the cleaning liquid.
As a further improvement of the invention, the screening device also comprises a waste material tray, and the waste liquid after being cleaned is discharged into the waste material tray.
The present invention also provides an active material automatic screening method, which is applicable to the active material automatic screening apparatus, and comprises: moving the pipette tip to suck the cleaning solution, and translating the stirring rod to enable the spray port of the stirring rod to be aligned with the sample pool; starting the air suction pump to perform air suction and air discharge actions, so that the magnetic bead emulsion and the substances to be screened are fully and uniformly mixed to perform affinity reaction; moving the magnetic stone to the lower end of the test material pool, opening the electromagnetic valve to enable magnetic bead particles to be adsorbed at the bottom of the test material pool, and fully combining active substances in the substances to be screened with the magnetic beads; the cleaning is repeated for a plurality of times, and the waste liquid is discharged into a waste material tray.
Compared with the prior art, the automatic active substance screening device and the automatic active substance screening method have the advantages that the sample device and the stirring device are arranged in the closed box body, so that the whole screening processes of injection, cleaning, separation and waste discharge are carried out in the closed box body, the temperature is controllable, the screening effect is good, and the cost is low.
Drawings
FIG. 1 is a schematic view of a sealed box of an automatic active material screening apparatus according to the present invention;
FIG. 2 is a schematic view showing the use of the automatic screening apparatus for active substances according to the present invention.
Detailed Description
Please refer to fig. 1, which is a schematic diagram of an automatic active material screening apparatus of the present invention, which is adapted to screen and separate an active material, the screening apparatus is a sealed box 1, and the whole screening process of injecting, cleaning, separating, and discharging is performed in the sealed box 1.
Referring to fig. 2, which is a schematic view of the automatic active material screening apparatus of the present invention, as shown in the figure, a horizontally movable sample device and a vertically movable stirring device are disposed in the sealed box 1, wherein the sample device includes a movable platform 10 and a sample cell 20, and the movable platform 10 drives the sample cell 20 to move horizontally;
the stirring device comprises a stirring rod 30 and a magnetic attraction device (not shown), the stirring rod 30 drives the magnetic attraction device to move up and down, a substance to be screened is put into the sample cell 20, and the substance to be screened is combined with the nano magnetic beads on the magnetic attraction device for screening and separation.
In this embodiment, the screening apparatus further includes a heater 40 and a cooler 50, which are disposed at the rear of the stirring rod 30 and move synchronously with the stirring rod 30.
In this embodiment, the screening apparatus further includes a liquid-transferring gun (not shown) disposed on the upper portion of the stirring rod 30, wherein the liquid-transferring gun is pumped and discharged by an air-sucking pump 60 to suck and discharge the cleaning liquid.
In this embodiment, the screening apparatus further includes a waste tray (not shown), and the waste liquid after cleaning is discharged into the waste tray.
The automatic screening method of the active substance comprises the following steps:
the method comprises the following steps: moving the pipette tip to suck the cleaning solution, and translating the stirring rod to enable the spray port of the stirring rod to be aligned with the sample pool;
step two: starting the air suction pump to perform air suction and air discharge actions, so that the magnetic bead emulsion and the substances to be screened are fully and uniformly mixed to perform affinity reaction;
step three: moving the magnetic stone to the lower end of the test material pool, opening the electromagnetic valve to enable magnetic bead particles to be adsorbed at the bottom of the test material pool, and fully combining active substances in the substances to be screened with the magnetic beads;
step four: the cleaning is repeated for a plurality of times, and the waste liquid is discharged into a waste material tray.
The embodiments and examples of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the embodiments and examples, and various changes can be made within the knowledge of those skilled in the art without departing from the spirit of the present invention.
Claims (4)
1. An active material automatic screening method, including utilizing the active material automatic screening device to carry on the automatic screening, characterized by that, the screening device is the closed box, there are removable horizontal assay device and removable vertical mixing unit in it, wherein:
the test material device comprises a moving platform and a test material pool, wherein the moving platform drives the test material pool to move horizontally;
the stirring device comprises a stirring rod and a magnet attraction device, wherein the stirring rod drives the magnet attraction device to move up and down, a substance to be screened is put into the sample cell, and the substance to be screened is combined with the nano magnetic beads on the magnet attraction device for screening and separation;
the screening method comprises the following steps:
the method comprises the following steps: moving the pipette tip to suck the cleaning solution, and translating the stirring rod to enable the spray port of the stirring rod to be aligned with the sample pool;
step two: starting the air suction pump to perform air suction and air discharge actions, so that the magnetic bead emulsion and the substances to be screened are fully and uniformly mixed to perform affinity reaction;
step three: moving the magnetic stone to the lower end of the test material pool, opening the electromagnetic valve to enable magnetic bead particles to be adsorbed at the bottom of the test material pool, and fully combining active substances in the substances to be screened with the magnetic beads;
step four: the cleaning is repeated for a plurality of times, and the waste liquid is discharged into a waste material tray.
2. The automatic active material screening method according to claim 1, wherein the screening apparatus further comprises a heater and a cooler, both provided at the rear of the stirring rod and moving in synchronization with the stirring rod.
3. The method for automatically screening active substances according to claim 1, wherein the screening device further comprises a pipette gun disposed at an upper portion of the stirring rod, the pipette gun performing suction and discharge of the cleaning solution by sucking and discharging air via a suction pump.
4. The method for automatically screening an active material according to claim 1, wherein the screening apparatus further comprises a waste tray into which the waste liquid after washing is discharged.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911046964.9A CN110882838A (en) | 2019-10-30 | 2019-10-30 | Method for automatically screening active substance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911046964.9A CN110882838A (en) | 2019-10-30 | 2019-10-30 | Method for automatically screening active substance |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110882838A true CN110882838A (en) | 2020-03-17 |
Family
ID=69746680
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911046964.9A Withdrawn CN110882838A (en) | 2019-10-30 | 2019-10-30 | Method for automatically screening active substance |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110882838A (en) |
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2019
- 2019-10-30 CN CN201911046964.9A patent/CN110882838A/en not_active Withdrawn
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| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20200317 |
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| WW01 | Invention patent application withdrawn after publication |