CN110879461A - Incident angle controllable light beam control method suitable for microscope light source - Google Patents
Incident angle controllable light beam control method suitable for microscope light source Download PDFInfo
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- CN110879461A CN110879461A CN201911271729.1A CN201911271729A CN110879461A CN 110879461 A CN110879461 A CN 110879461A CN 201911271729 A CN201911271729 A CN 201911271729A CN 110879461 A CN110879461 A CN 110879461A
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
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Abstract
The invention relates to a light beam control method with controllable incident angle suitable for a microscope light source, which is characterized in that incident light is directly adjusted, or an equivalent adjustable light source is formed by a light beam modulator, so that the incident light source forms a light source with different shapes, different brightness and different flicker frequencies; or the adjustable light source forms a light source with different shapes, different brightness and different flicker frequencies under the action of the light beam modulator. The invention flexibly combines the states of the light source or the equivalent light source and realizes the accurate control of the illumination light beam.
Description
Technical Field
The invention relates to a light beam control method with a controllable incident angle, which is suitable for a microscope light source.
Background
Critical illumination refers to a type of light collection system suitable for small projections where the collection optics image the light source onto or near the projection, and is referred to as critical illumination. Film projectors, slide projectors, and print enlargers all use critical lighting. Its advantages are high utilization rate of light energy and not easy uniform illumination.
However, the filament image of the light source is superposed with the plane of the detected object, so that the illumination of the detected object presents non-uniformity, and the part with the filament is bright; the part without filament is dim, which not only affects the quality of imaging, but also is not suitable for photomicrography, which is the main defect of critical illumination. The method for remedying the problem is to place a milky white and heat-absorbing color filter in front of the light source, so that the illumination becomes more uniform and the object to be detected is prevented from being damaged due to long-time irradiation of the light source.
The Kohler illumination can provide the best illumination mode for the sample, so that the sample can be uniformly and fully brightly illuminated, and dazzling glare can not be generated, therefore, modern laboratory microscope manufacturers recommend the method, and microscope users can explore all potential abilities of the microscope.
However, the incident angle of the light source affects the observation of the sample by the microscope, so that the sample cannot be accurately analyzed.
Disclosure of Invention
In order to solve the above technical problems, an object of the present invention is to provide a light beam control method suitable for a microscope light source with a controllable incident angle.
In order to achieve the purpose, the invention adopts the following technical scheme:
a light beam control method suitable for the controllable incident angle of the light source of the microscope, in incident the light source to regulate and control directly, or make the light source form an equivalent adjustable and controllable light source through the light beam modulator, make incident light source and adjustable and controllable light source form a light source with different shapes, different luminance, and different flicker frequency; or under the action of the light beam modulator, the light source forms a light source with different shapes, different brightness and different flicker frequencies.
Preferably, the controllable light source is integrated at the light source position through the beam modulator to form an equivalent controllable light source.
Preferably, the light beam control method is suitable for a microscope light source with a controllable incident angle, and the light source is a light source with a customizable shape, different positions of brightness and flicker frequency.
Preferably, in the method for controlling the light beam suitable for the microscope light source with the controllable incident angle, the shape of the light source realizes the shapes of different light sources by constructing different patterns on the lens array.
Preferably, in the method for controlling the light beam suitable for the microscope light source with the controllable incident angle, the brightness of different positions of the light source is realized by controlling the duty ratio of the position lens switch.
Preferably, in the light beam control method suitable for the microscope light source with the controllable incident angle, the flicker frequency of the light source is realized by rapidly switching on and off the micro-lens or rapidly adjusting the flicker of the light source.
Preferably, according to the light beam control method suitable for the microscope light source with the controllable incident angle, the shape of the light source, the brightness at different positions and the flicker frequency can be adjusted simultaneously.
By the scheme, the invention at least has the following advantages:
the invention makes the illumination light source incident at different positions of the back focal plane of the objective lens by modulating the light source/the light source, thereby achieving the purpose of controlling the incident angle of the light source. In addition, the state of the light source is flexibly combined, so that the illumination light beam is accurately controlled, and a high-quality intelligent imaging function is achieved.
The foregoing description is only an overview of the technical solutions of the present invention, and in order to make the technical solutions of the present invention more clearly understood and to implement them in accordance with the contents of the description, the following detailed description is given with reference to the preferred embodiments of the present invention and the accompanying drawings.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
FIG. 1 is a schematic diagram of the light source of the present invention incident on the left;
FIG. 2 is a schematic view of the light source of the present invention at intermediate incidence;
FIG. 3 is a schematic diagram of the right-hand incidence of the light source of the present invention;
fig. 4 is a complete schematic diagram of the incidence of the light source at different positions according to the present invention.
Detailed Description
The following detailed description of embodiments of the present invention is provided in connection with the accompanying drawings and examples. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
In order to make the technical solutions of the present invention better understood, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without making any creative effort, shall fall within the protection scope of the present invention.
Examples
A light beam control method suitable for microscope light source with controllable incident angle is to directly regulate the incident light source or to form an equivalent adjustable light source by a light beam modulator, so that the incident light source forms a light source with different shapes, different brightness and different flash frequencies, or the adjustable light source forms a light source with different shapes, different brightness and different flash frequencies under the action of the light beam modulator.
Preferably, the controllable light source is integrated at the light source position through the beam modulator to form an equivalent controllable light source.
The light source is a light source with a customizable shape, brightness at different positions and flicker frequency.
The shape of the light source in the invention realizes the shape of different light sources by constructing different patterns on the lens array.
The brightness of the light source at different positions is realized by controlling the ratio of the space to width of the position lens switch. The forming of the light source belongs to the technology known to those skilled in the art, and will not be described in detail here.
The flicker frequency of the light source is realized by rapidly switching the micro lens or rapidly adjusting the flicker of the light source. The above adjustments are made only with respect to the light source.
The shape of the light source, the brightness at different positions and the flicker frequency can be adjusted simultaneously.
The brightness of the different positions of the light source is realized by controlling the ratio of the occupation of width of the position lens switch, the flicker frequency of the light source is adjusted by rapidly switching the micro lens, or the flicker of the light source is rapidly adjusted to realize the flicker frequency and the shape of the light source, and the brightness and the flicker frequency at different positions can be adjusted at the same time, and the DLP/DMD of Texas instruments is adopted for control, which is a device known by the skilled person and will not be described in detail herein.
Example one
As shown in figure 1 of the drawings, in which,
the light source 1 is in the lower part, after passing through the lens a, to the conjugate focus 2, where there will be the first flare to control the intensity of the illumination. Behind the conjugate focus 2 there are a lens B and a lens C, between which there is a field of view that is smooth to adjust the extent of the field of view. After passing through the reflection/semi-transmission/semi-reflection lens, the light source is clearly imaged on a back focal plane 3 of the objective lens, and finally reaches the sample 5 in a left incident mode after passing through the objective lens 4, so that the sample 5 is clearly illuminated.
Example two
As shown in figure 2 of the drawings, in which,
the light source 1 is in the middle, passes through the lens a, and reaches the conjugate focus 2, where there is the first flare to control the intensity of the illumination. Behind the conjugate focus 2 there are a lens B and a lens C, between which there is a field of view that is smooth to adjust the extent of the field of view. After passing through the reflection/semi-transmission/semi-reflection lens, the light source is clearly imaged on a back focal plane 3 of the objective lens, and finally reaches the sample 5 in a middle incidence mode after passing through the objective lens 4, so that the sample 5 is clearly illuminated.
EXAMPLE III
As shown in figure 3 of the drawings,
the light source 1 is at the top, passes through the lens a, and reaches the conjugate focus 2, where there is the first flare to control the intensity of the illumination. Behind the conjugate focus 2 there are a lens B and a lens C, between which there is a field of view that is smooth to adjust the extent of the field of view. After passing through the reflection/semi-transmission/semi-reflection lens, the light source is clearly imaged on a back focal plane 3 of the objective lens, and finally reaches the sample in a right incidence mode after passing through the objective lens 4, so that the sample 5 is clearly illuminated.
Example four
As shown in figure 4 of the drawings,
the light source 7 comprises (upper, middle and lower light sources) and reaches the conjugate focus 2 after passing through the lens a, where there will be the first light to control the intensity of the illumination. Behind the conjugate focus 2 there are a lens B and a lens C, between which there is a field of view that is smooth to adjust the extent of the field of view. After the reflection/semi-transmission and semi-reflection lens, the light source clearly images on the back focal plane 3 of the objective lens, and after the objective lens 4, the camera assembly is adopted to observe simultaneously, so that the sample 5 is clearly illuminated.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, it should be noted that, for those skilled in the art, many modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.
Claims (7)
1. A light beam control method suitable for the controllable incident angle of the light source of the microscope, wherein in the direct regulation and control to the incident light source, or make the light source form an equivalent regulatable and controllable light source through the light beam modulator, make the incident light source and regulatable and controllable light source form a light source with different shapes, different luminance, and different flicker frequency; or under the action of the light beam modulator, the light source forms a light source with different shapes, different brightness and different flicker frequencies.
2. The method of claim 1, wherein the light beam control method comprises: the adjustable light source is combined at the position of the light source through the light beam modulator to form an equivalent adjustable light source.
3. The method of claim 1, wherein the light beam control method comprises: the light source is a light source with a customizable shape, brightness at different positions and flicker frequency.
4. A method of beam steering at a controllable angle of incidence for a light source for a microscope as claimed in any one of claims 1 to 3 wherein: the shape of the light source realizes the shape of different light sources by constructing different patterns on the lens array.
5. A method of beam steering at a controllable angle of incidence for a light source for a microscope as claimed in any one of claims 1 to 3 wherein: the brightness of the light source at different positions is realized by controlling the ratio of the occupation to the width of the position lens switch.
6. A method of beam steering at a controllable angle of incidence for a light source for a microscope as claimed in any one of claims 1 to 3 wherein: and the flicker frequency of the light source is realized by rapidly switching on and off the micro lens or rapidly adjusting the flicker of the light source.
7. A method of beam steering at a controllable angle of incidence for a light source for a microscope as claimed in any one of claims 1 to 3 wherein: the shape of the light source, the brightness at different positions and the flicker frequency can be adjusted simultaneously.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911271729.1A CN110879461A (en) | 2019-12-12 | 2019-12-12 | Incident angle controllable light beam control method suitable for microscope light source |
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| CN201911271729.1A CN110879461A (en) | 2019-12-12 | 2019-12-12 | Incident angle controllable light beam control method suitable for microscope light source |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN208766385U (en) * | 2018-02-13 | 2019-04-19 | 成都筋斗云影视传媒有限公司 | Stacking imaging system based on multi-angle illumination and Fourier domain coded modulation |
| CN110161670A (en) * | 2019-07-05 | 2019-08-23 | 麦克奥迪实业集团有限公司 | A kind of microscope illumination system of adjustable color |
| CN110441898A (en) * | 2019-08-21 | 2019-11-12 | 苏州精濑光电有限公司 | A kind of microscope and its control method |
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2019
- 2019-12-12 CN CN201911271729.1A patent/CN110879461A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN208766385U (en) * | 2018-02-13 | 2019-04-19 | 成都筋斗云影视传媒有限公司 | Stacking imaging system based on multi-angle illumination and Fourier domain coded modulation |
| CN110161670A (en) * | 2019-07-05 | 2019-08-23 | 麦克奥迪实业集团有限公司 | A kind of microscope illumination system of adjustable color |
| CN110441898A (en) * | 2019-08-21 | 2019-11-12 | 苏州精濑光电有限公司 | A kind of microscope and its control method |
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