CN110870798B

CN110870798B - Mouse tail intravenous route device

Info

Publication number: CN110870798B
Application number: CN201811015891.2A
Authority: CN
Inventors: 李泓胜
Original assignee: Shenyang Grenster Biotechnology Co ltd
Current assignee: Shenyang Grenster Biotechnology Co ltd
Priority date: 2018-09-01
Filing date: 2018-09-01
Publication date: 2022-06-07
Anticipated expiration: 2038-09-01
Also published as: CN110870798A

Abstract

A mouse tail vein injection method relates to an injection method, and comprises the steps that an elastic bolt (24) is screwed anticlockwise, a U-shaped clamping plate (12) is clamped and loosened, a placing tool (1) is rotated clockwise by hands to enable the placing tool (1) to be in a vertical placing state, the elastic bolt (24) is screwed clockwise to enable the U-shaped clamping plate (12) and a vertical plate (23) to be clamped and fastened, and the position of the placing tool (1) is fixed; the invention discloses a mouse tail vein injection method, which can realize the integration of mouse fixation and mouse placement and injection processes of different specifications and weights in the pharmacological experiment process, solves the problems that a fixing device has limitation on the weight of a mouse and the mouse needs hard plugging during filling in the traditional pharmacological experiment, is convenient and fast to operate, and optimizes the mouse filling mode and the mouse tail vein injection operation.

Description

Mouse tail intravenous route device

Technical Field

The invention relates to an injection device, in particular to a mouse tail vein injection device.

Background

The pharmacological experiment stage is divided into in vitro experiments, animal experiments and clinical experiments (human bodies), the essential stage before the development of the drugs is applied to the human bodies in the animal experiments, the drugs enter the human bodies, the influence is irreversible, the influence of the digestion often brings pain to the human bodies, even the life is damaged, and therefore, a large amount of animal experiments for a long time are needed to observe. Animal pharmacological experiments can effectively evaluate the indexes of the medicine such as effective rate, safety and the like. The method can help to determine the safe dosage range of the medicine application, avoid injury caused by overlarge dosage and is beneficial to avoiding or reducing the incidence rate of adverse reactions of the medicine in human bodies. Animal experiments become an indispensable important experimental technology in the field of biomedical science, and are widely applied.

Among mammalian experimental animals, human mice are used as an experiment as early as the 17 th century, and because the gene sequence of the mouse is similar to that of the human, some medical scientific research and clinical experiments are completed by the mouse, and many diseases which are difficult to cure by the human can be found by finding similar characters on the mouse, and the mouse is the mammalian experimental animal with the largest use amount, the most detailed research and the most use in various experimental researches. Mouse experiments are widely applied to experimental research in various fields, and particularly, the mouse experiments are more widely applied to the research of drug sensitivity, drug effect, pharmacokinetics and the like in large batches in the field of new drug research and development. The administration mode of the experimental mice comprises tail vein injection, enema administration, intraperitoneal injection, subcutaneous injection, lateral ventricle injection, nasal cavity administration and the like, wherein intravenous injection is one of the most common administration modes of the experimental mice.

In pharmacological experiments, because a mouse belongs to a living animal and is small in size, quick in action and uncontrollable in action, time and labor are wasted in the tail vein injection process of the mouse, and sometimes an experimenter can be bitten by the mouse, the mouse needs to be effectively fixed in the experiment process in order to protect the physical health of the experimenter and ensure the safe experiment. Although there is a fixing device to assist the pharmacological experiment of the mouse, the experimental operation has some disadvantages and problems, as follows:

1. when the tail vein injection of the mouse is carried out, most of the traditional fixing devices are used for fixing the mouse in a specially-made fixer, but the fixing effect is poor, and the effect of the auxiliary pharmacological experiment is poor, so that the experimental equipment capable of effectively fixing the mouse is in the shortage;

2. most of the existing mouse fixing devices are used for placing a mouse in a glass container, and then the tail of the mouse is left outside the glass container, so that the mouse can still move in the container within a small range in the experimental process and easily escape from the opening of the glass container, and the mouse needs to be placed back in the glass container by an experimenter, so that the experimenter is easily bitten by the mouse;

3. the used container for fixing the mouse has a fixed volume, can not be changed according to the weight and the size of the mouse, has great limitation in the practical process, and is not beneficial to the implementation of pharmacological experiments;

4. the tail of a mouse cannot be fixed by the conventional fixing device in the using process, and the tail of the mouse cannot be straightened in tail vein injection, so that an injection needle cannot accurately enter the tail vein of the mouse, the tail vein injection process is time-consuming and labor-consuming, and the tail vein injection efficiency is greatly reduced;

5. the existing fixing device, a cylinder for containing a mouse and an experiment fixing table matched with the cylinder are used together to perform experiments, so that the structure is complex and heavy, and the operation is inconvenient;

6. the currently used experiment fixing table cannot adjust the angle according to the experiment requirement, and is not beneficial to the implementation of pharmacological experiments;

7. before tail vein injection, mice must be weighed and then the dose administered is calculated based on the body weight of the mice. However, in the weighing process, the mouse often climbs on a balance tray and even escapes, so that the weighing is inaccurate, other containers are used for fixing the mouse in a certain moving range, and then the mouse and the containers are placed on the balance together for measurement, but the containers cannot fix all parts of the mouse, the mouse still moves in a small range, so that the result is inaccurate, the time for tail vein injection administration is delayed, and the experimental process is influenced.

In the process of carrying out pharmacological experiments on mice, the problems cannot be solved, and the mouse tail vein injection device capable of effectively fixing the mice in the pharmacological experiments is provided, and has important significance for medical scientific research and clinical experiments.

Disclosure of Invention

In order to overcome the defects in the background art, the invention discloses a mouse tail vein injection device, which can realize the integration of mouse fixation and mouse placement and injection processes of different specifications and body weights in the pharmacological experiment process, solves the problems that the fixing device has limitation on the body weight of a mouse and the mouse needs a hard plug during filling in the traditional pharmacological experiment, is convenient and fast to operate, and optimizes the mouse filling mode and the mouse tail vein injection operation.

The technical scheme for realizing the invention is as follows:

a mouse tail vein injection device comprises the following steps:

s1: the placing tool is vertically placed, and specifically comprises the following steps: screwing the loosening bolt anticlockwise, clamping the U-shaped clamping plate loosely, rotating the placing tool clockwise by hands to enable the placing tool to be in a vertical placing state, screwing the loosening bolt clockwise to enable the U-shaped clamping plate and the vertical plate to be clamped and fastened, and fixing the position of the placing tool;

s2: the clamping of the mouse is as follows: the plane plate is opened, the head fixing piece on the partition plate is taken out, the lower opening of the inner ring of the arc-shaped plastic plate is clamped and sleeved at the neck of a mouse, the mouse is fixed through the plug and the jack on two sides of the arc-shaped plastic plate, and after the head fixing piece is sleeved on the head fixing piece, an experimenter carries the mouse by grabbing the head fixing piece, so that the mouse is prevented from biting the hand of the experimenter in the mouse carrying process;

s3: mouse filling, specifically: putting the head fixing piece and the mouse into the opening on the partition plate, putting the head fixing piece into the clamping opening, realizing the limiting and clamping of the head fixing piece through a limiting edge on the outer wall of the head fixing piece so as to obtain the clamping and placing limit of the head of the mouse, penetrating the tail of the upper limb strapping tape through a through hole of the penetrating head, pulling outwards for adjustment, determining that the upper limb of the mouse is not easy to move and bounce when being bundled, then bundling the lower limb of the mouse through the lower limb strapping tape, and at the moment, penetrating the tail of the mouse through the through hole to the outside of the placing tool;

s4: the injection operation position of the fixing and fixing tool is as follows: screwing the loosening bolt anticlockwise, clamping and loosening the U-shaped clamping plate, rotating the placing tool anticlockwise by hands to enable the placing tool to rotate downwards, rotating to an inclination angle suitable for experimental operation, screwing the loosening bolt clockwise to enable the U-shaped clamping plate and the vertical plate to be clamped and fastened, and fixing the position of the placing tool;

s5: mouse tail injection: the mouse tail is placed in a groove which is arranged in the middle of a foam pad on the rear surface of the lower extension plate, is communicated with the front and the back of the foam pad and is connected with a through hole on the bottom surface of the placement device, the mouse tail is pressed to be placed in a plurality of open type buckles in the groove, the mouse tail is limited, and then the tail of a mouse is injected intravenously.

The mouse tail vein injection device has the beneficial effects that in the pharmacological experiment process, the mouse fixing and mouse placing and injection process integration meeting different specifications and weight sizes can be realized, and the inconvenience that the fixing device limits the weight of a mouse and needs to be hard plugged when the mouse is filled in the traditional pharmacological experiment is solved;

the head fixing piece is arranged in the cover body, the cover body can be clamped and sleeved at the neck of a mouse according to the embodiment of the mouse, the hanging of the mouse is convenient, convenience is provided for experimenters to carry and take the mouse, the hands of the experimenters are effectively prevented from being bitten by the mouse, great convenience is provided for the experimenters, the hanging of the mouse can be realized only by lifting the head of the mouse to clamp the neck of the mouse in a small opening at the lower part of an inner ring of an arc-shaped plastic plate when the mouse is filled, the mouse can be directly loaded in one step, the inconvenience that the mouse is hard-plugged in the cover body with a fixed space size in the prior art is avoided, and meanwhile, the hanging mode of the mouse can adapt to the filling of the mice with different sizes;

the upper limb strapping tape and the lower limb strapping tape are arranged, so that the binding and fixing can be carried out close to the skin according to different weights of mice, the mouse tail vein injection device is suitable for fixing the mice with various body types and weights, the upper limbs and the lower limbs of the mice can be effectively fixed in the fixing process, the mice can not move in the pharmacological experiment process, the tail vein injection in the pharmacological experiment can be favorably carried out, the head of the mice can be fixed, and the mice can be prevented from biting workers;

the angle between the cover body and the base can be adjusted by arranging the elastic bolts, so that the cover body is rotated to be vertically placed when a mouse is loaded, and then the mouse is rotated to be horizontal or slightly inclined to realize injection after the loading is finished, thereby avoiding the inconvenience that the traditional equipment needs to detach the cover body from the fixed table to load the mouse and then install the mouse to inject;

through fixing the tail of mouse in the ditch inslot to make its tail straightened, the going on of the mouse tail intravenous route of being convenient for can effectively avoid because of the swing of mouse afterbody, can't carry out the pharmacology experiment, can make the tail intravenous route process very light swift, greatly improve mouse tail intravenous route efficiency and have.

Through setting up U type splint, riser and elasticity bolt, can be according to the suitable injection angle that carries out tail vein injection of experimenter's demand adjustment, and clockwise rotation of accessible elasticity bolt behind the angle modulation, fasten more of centre gripping between U type splint and the riser, realize putting fixed and the fixed integration of angle modulation and mouse of utensil, make to put the utensil difficult rocking in pharmacology experimentation, be favorable to going on of tail vein injection, when avoiding traditional pharmacology experiment, need use the apparatus of dress mouse and the laboratory bench cooperation of fixed utensil to use and just can experiment, the structure is complicated heavy, the operation is inconvenient.

Through set up the through-hole and set up the slot that meets with the through-hole on the foam pad upper surface at the bottom surface of putting the utensil, in the pharmacology experimental process of tail vein injection, can fix the mouse tail, be convenient for intravenous injection, avoid because the mouse tail swing leads to can not accomplish the injection process or prick the mouse, be favorable to going on of pharmacology experiment, can be corresponding less for the pharmacology experiment process time.

Through setting gradually the baffle in the inside from the top down of putting the utensil, upper limbs strapping and low limbs strapping, and set up the lock neck mouth at the middle part of baffle, the fixed of mouse head can be realized to baffle and lock neck mouth, the head that makes the mouse is difficult for rocking, avoid the mouse to escape or sting the experimenter, cooperation upper limbs strapping and low limbs strapping use of mutually supporting, tie up the upper limbs and the low limbs of mouse, realize the mouse fixed in putting the utensil, avoid traditional pharmacology experiment, put the mouse in fixing device inside, the mouse does not have whole body to fix and still can carry out the drunkenness, be unfavorable for going on of tail vein injection, the staff of perhaps catching.

Set up respectively through the one end at upper limbs strapping and low limbs strapping and prick the head, and put the upper and lower lateral wall hole that the utensil wall set up, the head of penetrating of upper limbs strapping and low limbs strapping is fixed through upper and lower lateral wall hole and through the rivet, upper limbs strapping and low limbs strapping are the pine formula ribbon, can bind "next to the shin" to the different realization of body shape of mouse, it is limited to have solved traditional fixing device's volume, can not change with the size according to mouse self weight, make fixing device's use have the problem of a lot of limitations.

In addition, the invention can also assist in measuring the weight of the mouse, the mouse is fixed on the mouse tail vein injection device and then placed on a balance for measurement, the weight of the mouse per se can be obtained by subtracting the weight of the mouse tail vein injection device, the phenomenon that the experimental process is influenced because the mouse cannot be fixed and the tail vein injection administration time is delayed due to inaccurate measurement of the weight of the mouse when the weight of the mouse is measured in the traditional method can be avoided, the experimental error is reduced, the experimental precision is improved, and the convenience of the pharmacological experiment is greatly improved. The mouse fixing device has good stability for fixing the mouse, and is simple in structure and convenient to operate. Meanwhile, the experimenter can quickly, reasonably, stably and independently carry out tail vein injection on the mouse, and the experimenter is prevented from being scratched or bitten by the mouse, so that the pharmacological experiment can be carried out smoothly.

Drawings

The invention is further illustrated with reference to the following figures and examples.

FIG. 1 is a schematic structural view of a placement tool for a mouse tail intravenous injection device according to the present invention.

Fig. 2 is a schematic view of the overall structure of the mouse tail vein injection device of the present invention.

FIG. 3 is a schematic diagram of the structure of the partition board of the mouse tail intravenous injection device of the present invention.

Fig. 4 is a schematic structural view of a head fixing piece of the mouse tail intravenous injection device.

Fig. 5 is a schematic view of the lower board structure of the mouse tail intravenous injection device of the present invention.

In the figure, 1, a placing tool; 2. an opening; 3. a partition plate; 4. a head fixing member; 5. upper limb strapping tape; 6. a sidewall hole; 7. puncturing the head; 8. a hand-press handle; 9. lower limb strapping; 10. a through hole; 11. a forward extension panel; 12, U-shaped splints; 13. perforating; 14. a nut; 15. a flat plate; 16. hinge; 17. a handle; 18. a locking and absorbing piece; 19. a lock plug-in; 20. a lower extension plate; 21. a foam pad; 22. a trench; 23. a vertical plate; 24. tightening and loosening the bolts; 25. a base, 26. a card release opening; 27. an opening; 28. an arc-shaped plastic plate; 29. a jack; 30. a plug; 31. a limiting edge; 32, a first step of removing the first layer; an open type buckle.

Detailed Description

The present invention will now be described in further detail with reference to the accompanying drawings. These drawings are simplified schematic views illustrating only the basic structure of the present invention in a schematic manner, and thus show only the constitution related to the present invention.

A mouse tail vein injection device comprises the following steps:

s1: the placing tool 1 is vertically placed, and specifically comprises the following steps: screwing the turnbuckle 24 anticlockwise to clamp the U-shaped clamp plate 12 loosely, rotating the placing tool 1 clockwise by hand to enable the placing tool 1 to be in a vertical placing state, screwing the turnbuckle 24 clockwise to clamp and fasten the U-shaped clamp plate 12 and the vertical plate 23, and fixing the position of the placing tool 1;

s2: the clamping of the mouse is as follows: the plane plate 15 is opened, the head fixing piece 4 on the partition plate 3 is taken out, the neck of the mouse is clamped and sleeved at the neck of the mouse through a small opening at the lower part of the inner ring of the arc-shaped plastic plate 28, the mouse is fixed through the plug 30 and the jack 29 at the two sides of the arc-shaped plastic plate 28, and after the head fixing piece 4 is clamped and sleeved on the head fixing piece 4 of the mouse, an experimenter can carry the mouse by grasping the head fixing piece 4, so that the mouse is prevented from biting the hand of the experimenter in the mouse carrying process;

s3: mouse filling, specifically: putting the head fixing piece 4 and the mouse into the baffle plate 3 from the opening 27 and putting the head fixing piece 4 into the clamping opening 26, realizing the limiting clamping of the head fixing piece 4 through the limiting edge 31 on the outer wall of the head fixing piece 4 so as to obtain the clamping limiting position of the head of the mouse, penetrating the ribbon tail of the upper limb strapping tape 5 through the through hole of the piercing head 7, pulling outwards for adjustment, determining that the upper limb of the mouse is not easy to be moved when being pricked, then pricking the lower limb of the mouse through the lower limb strapping tape 9, and at the moment, penetrating the tail of the mouse out of the placing tool 1 through the through hole 10;

s4: the fixing and placing tool 1 comprises an injection operation position, specifically: screwing the turnbuckle 24 anticlockwise, clamping and loosening the U-shaped clamp plate 12, rotating the placing tool 1 anticlockwise by hands to enable the placing tool 1 to rotate downwards, rotating to an inclination angle suitable for experimental operation, screwing the turnbuckle 24 clockwise to enable the U-shaped clamp plate 12 and the vertical plate 23 to be clamped and fastened, and fixing the position of the placing tool 1;

s5: mouse tail injection: the mouse tail is placed in a groove 22 which is arranged in the middle of a foam pad 21 on the rear surface of a lower supporting plate 20, is communicated front and back and is connected with a through hole 10 on the bottom surface of a placing tool 1, the mouse tail is pressed to be placed in a plurality of open type buckles 32 in the groove 22, the mouse tail is limited, and then the tail of a mouse is injected intravenously.

The invention also provides a mouse tail vein injection device, which combines the structure shown in the attached figures 1-5 and comprises a bracket body, wherein the bracket body consists of a placing tool 1 and a base 25, the placing tool 1 is made of transparent glass material with a semicircular cylinder structure, one side edge of the placing tool 1 is movably connected with one side edge of a front end plane plate 15 through a plurality of hinges 16 which are vertically arranged, the other side edge of the placing tool 1 is connected with the other side edge of the plane plate 15 through a plurality of matched mortise locks which are vertically arranged, each mortise lock consists of a locking suction piece 18 and a locking insertion piece 19, the locking suction pieces 18 are respectively and fixedly connected with the upper part and the lower part of the front opening side edge of the placing tool 1, the locking insertion pieces 19 which are opposite to the locking suction pieces 18 are fixedly connected on one side edge surface of the plane plate 15, each locking insertion piece 19 is respectively provided with a plug which is inserted into each locking suction piece 18, each plug is respectively provided with a threaded hole, a bolt rod matched with the threaded hole on the plug is transversely inserted into the socket of each locking and sucking piece 18, the outer end of the bolt rod is connected with a rotating handle, the bolt rod in the locking and sucking piece 18 is screwed into the threaded hole on the plug of the locking and sucking piece 19 through screwing the rotating handle, so that the fastening and closing of the plane plate 15 and the placing tool 1 can be realized, the upper end of the placing tool 1 is provided with an arc-shaped opening 2, the opening 2 is arranged for facilitating the breathing of an experimental mouse and avoiding the death of the experimental mouse due to oxygen deficiency, a clapboard 3, an upper limb strapping band 5 and a lower limb strapping band 9 are sequentially arranged in the placing tool 1 from top to bottom, the front side of the clapboard 3 is provided with an opening 27, a clamping and releasing hole 26 is arranged in the opening 27, a head fixing piece 4 is clamped and released in the clamping and releasing hole 26, and the head fixing piece 4 consists of an arc-shaped plastic plate 28, the mouse is convenient for experimenters to carry the mouse, the head of the mouse can be prevented from shaking to escape or bite the hands of the experimenters, the upper limb strapping 5 and the lower limb strapping 9 are respectively provided with a threading head 7 at one end, each threading head 7 respectively passes through an upper side wall hole 6 and a lower side wall hole 6 which are arranged on the wall surface of the placing tool 1 and is respectively fixed in the upper side wall hole 6 and the lower side wall hole 6 through rivets, a hand handle 8 is movably connected on each threading head 7 respectively, the upper limb strapping 5 and the lower limb strapping 9 are releasable straps, the hand handle 8 can be directly punched and pressed, the binding tape can be repeatedly bound by automatically loosening the binding tape by the clamping teeth of the binding tape, a through hole 10 opposite to the head fixing part 4 is arranged on the bottom surface of the placing tool 1, the through hole 10 is arranged for enabling the tail of an experimental mouse to penetrate out of the placing tool 1, a front extension plate 11 is arranged at the front end of the bottom surface of the placing tool 1, an inverted U-shaped clamping plate 12 is fixedly connected to the middle part of the front edge of the lower surface of the front extension plate 11, the upper part of a vertical plate 23 fixedly connected with the upper surface of a base 25 is movably inserted into the U-shaped clamping plate 12, a loose bolt 24 sequentially penetrates through a through hole 13 on one side surface of the U-shaped clamping plate 12 and a through hole on the upper part of the vertical plate 23 to be in threaded connection with a nut 14 embedded in the through hole 13 on the other side surface of the U-shaped clamping plate 12, when the loose bolt 24 rotates clockwise, the U-shaped clamping plate 12 and the vertical plate 12 are clamped more tightly, and the fixing position of the placing tool 1 is not easy to rotate, when the elastic bolt 24 rotates anticlockwise, the clamping distance between the U-shaped clamping plate 12 and the vertical plate 12 is opened, the U-shaped clamping plate 12 is loosened, the angle of the placing tool 1 can be adjusted, the U-shaped clamping plate can be horizontal and vertical, the placing tool 1 can be fixed by rotating the elastic bolt 24 clockwise after the angle is determined, the lower end of the overlapped part of the bottom surface of the placing tool 1 and the front extension plate 11 is connected with the lower extension plate 20, the height of the lower extension plate 20 is lower than that of the vertical plate 23, when the placing tool 1 is erected, the lower extension plate 20 is erected simultaneously to avoid the lower extension plate 20 from touching the upper surface of the base 25, the rear surface of the lower extension plate 20 is glued with the foam pad 21, the middle part of the outer surface of the foam pad 21 is provided with the groove 22 which is penetrated from front to back and is connected with the through hole 10 of the bottom surface of the placing tool 1, the groove 22 on the lower extension plate 20 is orderly provided with a plurality of open type buckles 32 at intervals, after the tail of a mouse passes through hole 10, is placed in the groove 22, and the mouse tail is caught in the open type catch 32 by pressing the tail, thereby preventing the mouse tail from swinging.

When the mouse tail vein injection device is used for carrying out a pharmacological experiment, a white mouse for experiment is generally used for testing, when the device is used, the placing tool 1 is firstly ensured to be in a standing state, the plane plate 15 is just opposite to the front, the plane plate 15 is opened, the head fixing piece 4 on the partition plate 3 is taken out, the lower small opening of the inner ring of the arc-shaped plastic plate 28 is clamped at the neck of the mouse, the fixing is carried out through the plugs 30 and the jacks 29 on the two sides of the arc-shaped plastic plate 28, and after the head fixing piece 4 is sleeved on the head clamping piece 4 of the mouse, the mouse can be lifted by holding the head fixing piece 4 of the mouse, so that the mouse is effectively prevented from biting the hand of the experimenter in the mouse lifting process; putting the head fixing piece 4 and the mouse into the opening 27 on the clapboard 3 and putting the head fixing piece 4 in the clamping opening 26, realizing the limiting clamping of the head fixing piece 4 through the limiting edge 31 on the outer wall of the head fixing piece 4, thereby realizing the limiting clamping of the head of the mouse, clamping the head of the mouse above the clapboard 3 at the moment, leading the head of the mouse not to shake easily and avoiding escaping, penetrating the tail of the binding belt of the upper limb binding belt 5 through the perforation of the binding head 7, pulling outwards to adjust, determining the upper limb of the mouse to be bound and not to move easily, then binding the lower limb of the mouse through the lower limb binding belt 9, leading the tail of the mouse to penetrate through the through hole 10 to the outside of the placing tool 1, placing the tail of the mouse in the groove 22 in a proper way, covering the plane plate 15 and locking, screwing the elastic bolt 24 anticlockwise, clamping and loosening the U-shaped clamping plate 12, shifting the hand to the placing tool 1 to rotate downwards, rotating to the inclination angle suitable for experimental operation, clockwise screwing an elastic bolt 24 to clamp and fasten the U-shaped clamping plate 12 and the vertical plate 23, simultaneously driving the placing tool 1 to fix a position, wherein the plane plate 15 faces downwards and is opposite to the base 25, the experimental white mouse is in a belly-downward lying shape in the placing tool 1, the mouse tail is placed in the groove 22, and the mouse tail card is placed in a plurality of open type buckles 32 in the groove 22 by pressing the mouse tail, so that the swinging of the mouse tail is avoided, and the experimental test can be carried out on the tail of the white mouse; in the pharmacological experiment process, the mouse fixing and tail vein injection adjusting device is integrated at an angle suitable for experiments, and the problems that in the traditional pharmacological experiment, the fixing device is complex in structure and complex to operate, and the angle of an operation table cannot be changed are solved.

In light of the foregoing description of the preferred embodiment of the present invention, many modifications and variations can be made by the worker in the light of the above teachings without departing from the spirit of the invention. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.

Claims

1. A mouse tail vein injection device is characterized in that: the bracket comprises a bracket body, wherein the bracket body consists of a placing tool (1) and a base (25), one side edge of the placing tool is movably connected with one side edge of a front end plane plate through a plurality of hinges which are vertically arranged, the other side edge of the placing tool is connected with the other side edge of the front end plane plate through a plurality of matched mortise locks which are vertically arranged, and an arc-shaped opening is arranged at the upper end of the placing tool; a clapboard, an upper limb strapping tape and a lower limb strapping tape are sequentially arranged in the placing tool from top to bottom, an opening is arranged on the front side of the clapboard, and a clamping opening is arranged in the opening; a head fixing piece is clamped in the clamping opening and consists of an arc-shaped plastic plate, a limiting edge is arranged on the lower side of the outer side surface of the arc-shaped plastic plate, penetrating heads are respectively arranged at one ends of the upper limb strapping tape and the lower limb strapping tape, each penetrating head respectively penetrates through an upper side wall hole and a lower side wall hole which are arranged on the wall surface of the placing tool and is respectively fixed in the upper side wall hole and the lower side wall hole through rivets, a hand handle is respectively and movably connected to each penetrating head, and a through hole which is opposite to the head fixing piece is arranged on the bottom surface of the placing tool; the front end of the bottom surface of the placing tool is provided with a front extension plate, the front middle part of the front edge of the lower surface of the front extension plate is fixedly connected with an inverted U-shaped clamp plate, the upper part of a vertical plate fixedly connected with the upper surface of the base, which is provided with a through hole, is movably inserted into the U-shaped clamp plate, a tightening bolt sequentially penetrates through the through hole on one side surface of the U-shaped clamp plate and the through hole on the upper part of the vertical plate to be in threaded connection with a nut embedded in the through hole on the other side surface of the U-shaped clamp plate, the lower end of the overlapped part of the bottom surface of the placing tool and the front extension plate is connected with a lower extension plate, the height of the lower extension plate is lower than that of the vertical plate, the rear surface of the lower extension plate is glued with a foam pad, the middle part of the outer surface of the foam pad is provided with a groove which is penetrated from front to back and is connected with the through hole on the bottom surface, and a plurality of open type buckles are arranged on the lower extension plate at intervals in order;

the operation method of the mouse tail vein injection device comprises the following steps:

s1: the placing tool (1) is vertically placed, and specifically comprises the following steps: screwing a turnbuckle (24) anticlockwise, clamping and loosening the U-shaped clamping plate (12), rotating the placing tool (1) clockwise by hands to enable the placing tool (1) to be in a vertical placing state, screwing the turnbuckle (24) clockwise to enable the U-shaped clamping plate (12) and the vertical plate (23) to be clamped and fastened, and fixing the position of the placing tool (1);

s2: the clamping of the mouse is as follows: the front end plane plate (15) is opened, the head fixing piece (4) on the partition plate (3) is taken out, the lower part of the inner ring of the arc-shaped plastic plate (28) is clamped and sleeved at the neck of a mouse through a small opening at the lower part of the inner ring of the arc-shaped plastic plate (28), and the head fixing piece (4) is fixed through a plug (30) and a jack (29) at two sides of the arc-shaped plastic plate (28), so that an experimenter can take the mouse by grabbing the head fixing piece (4) after the mouse head clamping piece (4) is sleeved on the head fixing piece (4), and the mouse is prevented from biting the hand of the experimenter;

s3: mouse filling, specifically: putting the head fixing piece (4) and the mouse into the clamping opening (26) from the opening (27) on the partition plate (3), and putting the head fixing piece (4) in the clamping opening, realizing the limiting clamping of the head fixing piece (4) through a limiting edge (31) on the outer wall of the head fixing piece (4), thereby obtaining the clamping limiting position of the head of the mouse, enabling the tail of the binding belt of the upper limb binding belt (5) to penetrate through a through hole penetrating through the binding head (7), pulling outwards for adjustment, determining that the upper limb of the mouse is not easy to move and bounce, and then binding the lower limb of the mouse through the lower limb binding belt (9), wherein the tail of the mouse can penetrate through the through hole (10) to penetrate out of the placing tool (1);

s4: the injection operation position of the fixing and placing tool (1) is as follows: screwing a turnbuckle (24) anticlockwise, clamping and loosening the U-shaped clamping plate (12), rotating the placing tool (1) anticlockwise by hands to enable the placing tool (1) to rotate downwards, rotating to an inclination angle suitable for experimental operation, screwing the turnbuckle (24) clockwise to enable the U-shaped clamping plate (12) and the vertical plate (23) to be clamped and fastened, and fixing the position of the placing tool (1);

s5: mouse tail injection: the mouse tail is placed in a groove (22) which is arranged in the middle of a foam pad (21) on the rear surface of a lower supporting plate (20), is communicated with the front and the back and is connected with a through hole (10) on the bottom surface of a placing tool (1), the mouse tail is pressed to be placed in a plurality of open type buckles (32) in the groove (22), the mouse tail is limited, and then the tail of a mouse is injected intravenously.