CN110862481A - A kind of self-healing hydrogel based on hydrophobic interaction and preparation method thereof - Google Patents

Abstract

The invention relates to a self-healing hydrogel based on hydrophobic interaction and a preparation method thereof, belonging to the technical field of biomedical materials. It can be prepared by the following method: with 96% octadecyl methacrylate (C ₁₈ ) as hydrophobic agent, C ₁₈ is dissolved in NaCl-surfactant system, and N-isopropylacrylamide (NIPA ) and acrylamide (AAm) as raw materials, with N,N,N',N'-tetramethylethylenediamine (TEMED) as coagulant and ammonium persulfate (APS) as initiator, by micellar copolymerization method Preparation of self-healing hydrogels. The hydrogel has good mechanical properties, biocompatibility and biodegradability, and excellent self-healing ability, and can be used as a material in the field of biomedicine.

Description

A kind of self-healing hydrogel based on hydrophobic interaction and preparation method thereof

technical field

The invention relates to a preparation method of a self-healing hydrogel, belonging to the technical field of biomedical materials.

Background technique

As a material with good biocompatibility, hydrogel can be endowed with a variety of functions (such as degradability, stimuli responsiveness, etc.), it can interact with cells to guide the expression of cell functions and further form new It can also store and release drugs, so it has great potential application value in the fields of tissue adhesives, drug controlled storage and release, etc. However, traditional hydrogels have problems such as inability to self-heal after damage and poor mechanical strength, which greatly limits their application in the biomedical field. Self-healing materials are a class of smart materials with inherent automatic damage repair capabilities. There are two existing healing methods. One is to self-trigger the healing process through external factors such as light, heat, pH or catalysts, but this process not only consumes Many energies, and their healing process is complex, especially in the field of biomedicine, which greatly limits the application of self-healing hydrogels; , self-healing has become one of the current research hotspots of smart materials. With the development of science and technology, scientists have done a lot of research on the blocking of self-healing hydrogels, but from the current research situation, it is necessary to make self-healing hydrogels meet the requirements of the biomedical field. required, which requires them to have good biocompatibility, biodegradability and non-toxicity, and self-repair without external force.

Self-healing hydrogels that heal without external force can be divided into physical self-healing hydrogels and chemical self-healing hydrogels. Chemical self-healing hydrogels usually self-heal through reversible covalent bonds, and physical self-healing hydrogels. Healing hydrogels generally self-heal through hydrogen bonds, electrostatic interactions, and hydrophobic interactions. The self-healing mechanism is based on the reversible action of dynamic chemical bonds, which requires complex chemical reactions, and the preparation process is cumbersome, and most of them use organic solvents as the reaction system, which is prone to environmental pollution and difficult disposal of waste liquids. Physically acting self-healing hydrogels can overcome these shortcomings, and their cross-linking points have transient characteristics and are in the dynamic equilibrium process of cross-linking and de-cross-linking, and the internal stress of the gel is also in this dynamic equilibrium process. Obtaining Songshe, the physical gel has a greater advantage in the preparation of gels with self-healing properties.

SUMMARY OF THE INVENTION

The object of the present invention is to provide a self-healing hydrogel based on hydrophobic interaction, which has good mechanical strength, biocompatibility and biodegradability, and excellent self-healing ability. The self-healing hydrogel based on hydrophobic interaction provided by the present invention can be prepared by the following methods:

Dissolve a certain amount of NaCl (0.7M) and surfactant in 9.8mL of distilled water at 25°C, stir with a stirrer to obtain a transparent solution, take 88uL of 96% octadecyl methacrylate (C ₁₈ ) Dissolve in NaCl-surfactant system, continue to stir for 2h, and seal with plastic wrap to prevent water evaporation during stirring. After C ₁₈ was dissolved, 0.5 g of N-isopropylacrylamide (NIPA) and 0.5 g of acrylamide (AAm) were added to react for 0.5 h. 25uL of N,N,N',N'-tetramethylethylenediamine (TEMED) and 0.1 mL of ammonium persulfate (APS) (0.08 g/mL) were added to the reaction solution and stirred well. The obtained solution was poured into a glass plate and allowed to stand for 24 hours, and the obtained gel was placed in a plastic wrap to prevent volatilization of the gel moisture.

Another object of the present invention is to provide a preparation method of self-healing hydrogel based on hydrophobic interaction, the preparation method comprising the following steps:

Dissolve a certain amount of NaCl (0.7M) and surfactant in 9.8mL of distilled water at 25°C, stir with a stirrer to obtain a transparent solution, take 88uL of 96% octadecyl methacrylate (C ₁₈ ) Dissolve in NaCl-surfactant system, continue to stir for 2h, and seal with plastic wrap to prevent water evaporation during stirring. After C ₁₈ was dissolved, 0.5 g of N-isopropylacrylamide (NIPA) and 0.5 g of acrylamide (AAm) were added to react for 0.5 h. 25uL of N,N,N',N'-tetramethylethylenediamine (TEMED) and 0.1 mL of ammonium persulfate (APS) (0.08 g/mL) were added to the reaction solution and stirred well. The obtained solution was poured into a glass plate and allowed to stand for 24 hours, and the obtained gel was placed in a plastic wrap to prevent volatilization of the gel moisture.

beneficial effect

The self-healing hydrogel based on the hydrophobic interaction provided by the present invention has good mechanical properties, biocompatibility, biodegradability, and excellent self-healing ability, and can be applied to the field of biomedicine.

Detailed ways

The technical solutions of the present invention will be further illustrated and described below through specific embodiments.

Example 1:

Dissolve NaCl (0.7M) and sodium dodecyl sulfate (SDS) (0.17M) in 9.8mL of distilled water at 25°C, stir with a stirrer to obtain a clear solution, take 88uL of 96% methacrylic acid The octaalkyl ester (C ₁₈ ) was dissolved in the NaCl-surfactant system, and the stirring was continued for 2 h. During the stirring, the seal was sealed with plastic wrap to prevent water evaporation. After C ₁₈ was dissolved, 0.5 g of N-isopropylacrylamide (NIPA) and 0.5 g of acrylamide (AAm) were added to react for 0.5 h. 25uL of N,N,N',N'-tetramethylethylenediamine (TEMED) and 0.1 mL of ammonium persulfate (APS) (0.08 g/mL) were added to the reaction solution and stirred well. The obtained solution was poured into a glass plate and allowed to stand for 24 hours, and the obtained gel was placed in a plastic wrap to prevent volatilization of the gel moisture.

Example 2:

Dissolve NaCl (0.7M) and cetyltrimethylammonium chloride (CTAC) (0.24M) in 9.8mL of distilled water at 25°C, stir with a stirrer to obtain a transparent solution, take 88uL of 96% Octadecyl methacrylate (C ₁₈ ) was dissolved in the NaCl-surfactant system, stirring was continued for 2 h, and during the stirring period, the seal was sealed with plastic wrap to prevent water evaporation. After C ₁₈ was dissolved, 0.5 g of N-isopropylacrylamide (NIPA) and 0.5 g of acrylamide (AAm) were added to react for 0.5 h. 25uL of N,N,N',N'-tetramethylethylenediamine (TEMED) and 0.1 mL of ammonium persulfate (APS) (0.08 g/mL) were added to the reaction solution and stirred well. The obtained solution was poured into a glass plate and allowed to stand for 24 hours, and the obtained gel was placed in a plastic wrap to prevent volatilization of the gel moisture.

Example 3:

Dissolve NaCl (0.7M) and Tween 80 (TW80) (0.31M) in 9.8mL of distilled water at 25°C, stir with a stirrer to obtain a transparent solution, take 88uL of 96% octadecyl methacrylate The ester (C ₁₈ ) was dissolved in the NaCl-surfactant system, stirring was continued for 2 h, and during stirring, the seal was sealed with plastic wrap to prevent water evaporation. After C ₁₈ was dissolved, 0.5 g of N-isopropylacrylamide (NIPA) and 0.5 g of acrylamide (AAm) were added to react for 0.5 h. 25uL of N,N,N',N'-tetramethylethylenediamine (TEMED) and 0.1 mL of ammonium persulfate (APS) (0.08 g/mL) were added to the reaction solution and stirred well. The obtained solution was poured into a glass plate and allowed to stand for 24 hours, and the obtained gel was placed in a plastic wrap to prevent volatilization of the gel moisture.

Example 4:

Dissolve NaCl (0.7M) and sodium oleate (NAOL) (0.17M) in 9.8mL of distilled water at 25°C, stir with a stirrer to obtain a transparent solution, take 88uL of 96% octadecyl methacrylate The ester (C ₁₈ ) was dissolved in the NaCl-surfactant system, stirring was continued for 2 h, and during stirring, the seal was sealed with plastic wrap to prevent water evaporation. After C ₁₈ was dissolved, 0.5 g of N-isopropylacrylamide (NIPA) and 0.5 g of acrylamide (AAm) were added to react for 0.5 h. 25uL of N,N,N',N'-tetramethylethylenediamine (TEMED) and 0.1 mL of ammonium persulfate (APS) (0.08 g/mL) were added to the reaction solution and stirred well. The obtained solution was poured into a glass plate and allowed to stand for 24 hours, and the obtained gel was placed in a plastic wrap to prevent volatilization of the gel moisture.

Example 5:

Dissolve NaCl (0.7M) and sodium dodecyl sulfate (SDS) (0.24M) in 9.8mL of distilled water at 25°C, stir with a stirrer to obtain a clear solution, take 88uL of 96% methacrylic acid ten The octaalkyl ester (C ₁₈ ) was dissolved in the NaCl-surfactant system, and the stirring was continued for 2 h. During the stirring, the seal was sealed with plastic wrap to prevent water evaporation. After C ₁₈ was dissolved, 0.5 g of N-isopropylacrylamide (NIPA) and 0.5 g of acrylamide (AAm) were added to react for 0.5 h. 25uL of N,N,N',N'-tetramethylethylenediamine (TEMED) and 0.1 mL of ammonium persulfate (APS) (0.08 g/mL) were added to the reaction solution and stirred well. The obtained solution was poured into a glass plate and allowed to stand for 24 hours, and the obtained gel was placed in a plastic wrap to prevent volatilization of the gel moisture.

Example 6:

Dissolve NaCl (0.7M) and cetyltrimethylammonium chloride (CTAC) (0.31M) in 9.8mL of distilled water at 25°C, stir with a stirrer to obtain a transparent solution, take 88uL of 96% Octadecyl methacrylate (C ₁₈ ) was dissolved in the NaCl-surfactant system, and stirring was continued for 2 h. During stirring, the seal was sealed with plastic wrap to prevent water evaporation. After C ₁₈ was dissolved, 0.5 g of N-isopropylacrylamide (NIPA) and 0.5 g of acrylamide (AAm) were added to react for 0.5 h. 25uL of N,N,N',N'-tetramethylethylenediamine (TEMED) and 0.1 mL of ammonium persulfate (APS) (0.08 g/mL) were added to the reaction solution and stirred well. The obtained solution was poured into a glass plate, and allowed to stand for 24 hours, and the obtained gel was placed in a plastic wrap to prevent volatilization of the gel moisture.

Claims (6)

1. A self-healing hydrogel based on hydrophobic interaction and a preparation method thereof, characterized in that it has good mechanical properties, biocompatibility and biodegradability, excellent self-healing ability, and the gel can be obtained from the following: Method made: Dissolve a certain amount of NaCl (0.7M) and surfactant in 9.8mL of distilled water at 25°C, stir with a stirrer to obtain a transparent solution, take 88uL of 96% octadecyl methacrylate (C ₁₈ ) Dissolve in NaCl-surfactant system, continue stirring for 2h, seal with plastic wrap during stirring to prevent water evaporation, after _C18 dissolves, add 0.5g N-isopropylacrylamide (NIPA) and 0.5g acrylamide (AAm) Reaction 0.5h. 25uL of N,N,N',N'-tetramethylethylenediamine (TEMED) and 0.1mL of ammonium persulfate (APS) (0.08g/mL) were added to the reaction solution, stirred well, and the resulting solution was poured into In a glass plate, let stand for 24 hours, and the prepared gel is placed in a plastic wrap to prevent the volatilization of the gel moisture. 2. The hydrogel according to claim 1, characterized in that it has good mechanical properties, biocompatibility and biodegradability, and excellent self-healing ability, and can be applied in the field of biomedicine. 3. A preparation method of self-healing hydrogel based on hydrophobic interaction, comprising the following steps: Dissolve a certain amount of NaCl (0.7M) and surfactant in 9.8mL of distilled water at 25°C, stir with a stirrer to obtain a transparent solution, take 88uL of 96% octadecyl methacrylate (C ₁₈ ) Dissolve in NaCl-surfactant system, continue stirring for 2h, seal with plastic wrap during stirring to prevent water evaporation, after _C18 dissolves, add 0.5g N-isopropylacrylamide (NIPA) and 0.5g acrylamide (AAm) Reaction 0.5h. 25uL N,N,N',N'-tetramethylethylenediamine (TEMED) and 0.1mL ammonium persulfate (APS) (0.08g/mL) were added to the reaction solution, stirred well, and the resulting solution was poured into In a glass plate, let stand for 24 hours, and the prepared gel is placed in a plastic wrap to prevent the volatilization of the gel moisture. 4. A method for preparing a self-healing hydrogel based on hydrophobic interaction according to claim 3, wherein the concentration of NaCl in the step is 0.7M. 5. a kind of self-healing hydrogel preparation method based on hydrophobic interaction according to claim 3, is characterized in that in described step, surfactant is sodium dodecyl sulfate (SDS), hexadecyl trimethyl Ammonium chloride (CTAC), sodium oleate (NAOL) Tween 80 (TW80), the concentration is 0.17M ~ 0.31M. 6 . The method for preparing a self-healing hydrogel based on hydrophobic interaction according to claim 3 , wherein in the step, NIPA:AAm=1:1. 7 .