CN110845001A - Method for treating low-carbon urban sewage by polymer-driven denitrification - Google Patents

Method for treating low-carbon urban sewage by polymer-driven denitrification Download PDF

Info

Publication number
CN110845001A
CN110845001A CN201911046799.7A CN201911046799A CN110845001A CN 110845001 A CN110845001 A CN 110845001A CN 201911046799 A CN201911046799 A CN 201911046799A CN 110845001 A CN110845001 A CN 110845001A
Authority
CN
China
Prior art keywords
period
sewage
aerobic
cod
stage
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201911046799.7A
Other languages
Chinese (zh)
Other versions
CN110845001B (en
Inventor
杨国靖
王宇
唐力
张妮
夏静芬
徐佳杰
吴亦馨
张超妍
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zhejiang Wanli University
Zhejiang Wanli College
Original Assignee
Zhejiang Wanli College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zhejiang Wanli College filed Critical Zhejiang Wanli College
Priority to CN201911046799.7A priority Critical patent/CN110845001B/en
Publication of CN110845001A publication Critical patent/CN110845001A/en
Application granted granted Critical
Publication of CN110845001B publication Critical patent/CN110845001B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/02—Aerobic processes
    • C02F3/12—Activated sludge processes
    • C02F3/1236—Particular type of activated sludge installations
    • C02F3/1263—Sequencing batch reactors [SBR]
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/02—Aerobic processes
    • C02F3/12—Activated sludge processes
    • C02F3/1205—Particular type of activated sludge processes
    • C02F3/121—Multistep treatment
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/30—Aerobic and anaerobic processes
    • C02F3/301—Aerobic and anaerobic treatment in the same reactor
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/10—Biological treatment of water, waste water, or sewage

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Microbiology (AREA)
  • Hydrology & Water Resources (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)

Abstract

The invention relates to a method for treating low-carbon urban sewage by polymer-driven denitrification, belonging to the technical field of sewage treatment. The invention relates to a method for treating low-carbon urban sewage by polymer-driven denitrification, which comprises the following steps: s1, inoculation: inoculating flocculent activated sludge into a Sequencing Batch Reactor (SBR), and aerating; s2, operation regulation: the method comprises a domestication period, an adjustment period and an optimization period, wherein the domestication period comprises a first aerobic stage taking high-COD sewage as inflow water and a second aerobic stage taking low-COD sewage as inflow water. The method can quickly and efficiently realize the low-carbon urban sewage denitrification treatment driven by the inner polymer.

Description

Method for treating low-carbon urban sewage by polymer-driven denitrification
Technical Field
The invention belongs to the field of sewage treatment, and relates to a method for treating low-carbon urban sewage by polymer-driven denitrification.
Background
The biological sewage treatment technology has the characteristics of low treatment cost, good treatment effect, small environmental side effect and the like, is known to be an economical and effective method in the current water pollution control and treatment process, and is widely used for removing pollutants such as nitrogen, phosphorus and the like in urban domestic sewage.
The conventional and popular sewage biological treatment processes can be classified into two major types, a suspended growth system and an attached growth system, according to the existence state of microorganisms. The former is represented by an activated sludge process, and has the defects of low sludge concentration, low volume load and sensitivity to impact load; the reactor and the secondary sedimentation tank occupy large areas; easily causes sludge bulking, has much excess sludge, has large treatment difficulty and the like. The latter is represented by a biofilm method, and has the defect of large investment in carriers or fillers; under the operating condition, the biomass in unit volume is relatively constant, the sludge load is difficult to increase, and the volume of the carrier or the filler is larger than that of the actual reactor. Both of these two systems have the disadvantages of high capital investment, large floor space, high operation and management cost, and the like which must be overcome.
At present, the research and development of a compact, integrated, efficient and energy-saving novel process becomes a hotspot, particularly the aerobic granular sludge process is taken as a highlight, the process belongs to the category of microorganism self-immobilization, and has the obvious advantages of small occupied area, high biomass, high volume load, good sludge precipitation performance, low sludge yield, no need of a precipitation tank, no additional investment, rich microorganism phases, enrichment of enriched and strengthened functional flora and the like. Therefore, the popularization and the application of the aerobic granular sludge process can greatly improve the traditional process mode, and have important significance.
The aerobic granular sludge is a smooth, compact and regular spherical microorganism aggregate formed by mutual agglomeration of microorganisms under the induction of specific conditions, and compared with the traditional flocculent activated sludge, the aerobic granular sludge is concerned and favored by researchers due to the advantages of high settling speed, easiness in enriching functional microorganisms, small occupied volume of sewage treatment and the like. Because the inside of the aerobic granular sludge has mass transfer obstruction to the substrate and dissolved oxygen, three areas of aerobic, anoxic and anaerobic are naturally formed along the mass transfer direction. The unique spatial configuration can meet the requirements of aerobic, facultative aerobic and anaerobic microorganisms on the growth environment at the same time, so that various microorganisms find an ecological site suitable for the growth of the microorganisms to form a micro ecological system, and the synchronous denitrification and dephosphorization in the same system become possible. In addition, the microorganisms in the granular sludge are closely adhered to each other, so that the material exchange distance among bacteria is greatly shortened, the synergistic effect of functional bacteria is improved, and the degradation and removal of pollutants are promoted.
Compared with flocculent activated sludge, aerobic granular sludge has a plurality of advantages, but the formation process is complex and the mechanism is not clear. The characteristic that urban sewage in China mostly has low organic matter concentration (average COD is lower than 200mg/L) is a bottleneck of the engineering application of the aerobic granular sludge technology in China, because the low organic load is not beneficial to the formation of the aerobic granular sludge. The COD value of the urban sewage is low, the COD/N/P ratio of the sewage is low, and the shortage of carbon source becomes a limiting factor of denitrification and biological phosphorus removal. The aerobic granular sludge cultured by the high COD simulated sewage is applied to the actual urban sewage treatment, an external carbon source is needed, the cost of sewage treatment is increased, and the method has great limitation.
The main technical theories of the sewage biological treatment process comprise synchronous nitrification and denitrification, short-cut nitrification and denitrification, anaerobic ammonia oxidation, denitrification and phosphorus removal and the like.
The phenomenon in which nitrification and denitrification processes occur simultaneously in the same biological sewage treatment system under aerobic conditions is called Simultaneous Nitrification and Denitrification (SND). The mechanism of SND can be roughly summarized into the following two theories: one is a macroscopic theory starting from the environmental impact conditions under which the microorganisms live, and the other is a microenvironment theory starting from the physiological characteristics of the microorganisms themselves.
The macroscopic theory considers that under aerobic conditions, the phenomenon of uneven dissolved oxygen distribution exists in the same biological reaction system, so that an aerobic zone and an anoxic zone can exist simultaneously. In the biological treatment system in the aeration stage, because the microbial flocs have dissolved oxygen limitation, aerobic microorganisms are mainly distributed on the surface layer for nitrification, and the dissolved oxygen concentration in the inner layer is lower, so that a microenvironment condition is provided for the growth of denitrifying bacteria and denitrification.
The micro theory considers that under the aerobic condition, denitrification is realized by the combined action of aerobic denitrifying bacteria and heterotrophic nitrifying bacteria, nitrite, nitrate and oxygen are used as electron acceptors, and the generated nitrite nitrogen and nitrate nitrogen are reduced into nitrogen.
Compared with the traditional process, the SND process has the greatest advantages that the SND process can replace two stages of the traditional biological nitrification and denitrification, alkali does not need to be added for neutralization of a reaction system, the reaction time is shortened, the volume of a reactor can be saved, and the cost of sewage treatment is reduced.
The short-cut nitrification and denitrification means that the nitrification reaction process is carried out to the nitrosation stage, excessive nitrite nitrogen is accumulated, and then NO is directly used2 -As an electron acceptor into the denitrification stage. The key point for the realization of the shortcut nitrification-denitrification technology lies in how to excessively accumulate nitrite nitrogen produced in the nitrification process and not further oxidize the nitrite nitrogen into nitrate nitrogen on a macroscopic scale and in how to effectively control the AOB to be higher than the NOB in the number or activity of flora. In recent years, researchers have conducted a great deal of research on the control conditions for excessive accumulation of nitrite nitrogen, and the results show that the conditions for controlling the accumulation of nitrite nitrogen are very complicated, such as temperature, dissolved oxygen concentration, organic load, toxic and harmful substances, pH, sludge age and the like all affect the accumulation of nitrite nitrogen, and it is very difficult for the nitration reaction to stay in the nitrosation stage. At present, the control for realizing the short-cut nitrification is mainly started from the aspects of regulating and controlling the concentration of free ammonia, the pH value and the dissolved oxygen concentration, and the regulating and controlling methods are all based on that AOB and NOB have different biological activities under the same condition, and simultaneously, AOB growth is promoted and NOB growth is inhibited as far as possibleAnd (4) conditions.
Compared with the traditional process, the short-cut nitrification and denitrification process can not only save 40 percent of carbon source and 25 percent of oxygen demand, but also improve the nitrification rate and reduce the nitrification reaction time, thereby reducing the volume of the reactor by 30 to 40 percent and reducing the generation amount of sludge. Therefore, the short-cut nitrification and denitrification process can greatly save the construction cost and the operation cost, and has profound significance on the treatment of the sewage with low carbon-nitrogen ratio.
Anaerobic ammonia oxidation is that under anaerobic and anoxic conditions, microorganisms use NO2 -Or NO3 -Is an electron acceptor, and oxidizes ammonia nitrogen to generate nitrogen. The key control element for successful implementation of anammox is to ensure retention of certain abundant anammox bacteria in a biological reaction system. Numerous studies in recent years have also shown that factors such as organic loading, dissolved oxygen concentration, temperature, sludge age, etc. all affect the growth of anammox bacteria, and thus the creation of suitable reaction conditions is a prerequisite for the efficient operation of the process. Compared with the traditional denitrification process, the anaerobic ammonia oxidation process has the advantages of no need of oxygen supply and addition of an external carbon source, less excess sludge, strong affinity with a polluted substrate in the reaction process and the like, and is the current economic sewage denitrification technology. However, the existing anammox process is limited to the treatment of wastewater with high ammonia nitrogen content in the field of sewage treatment, and the anaerobic ammonia oxidizing bacteria (autotrophic microorganisms) have harsh living conditions and are not easy to be enriched and cultured in the sewage treatment process, so that the related defects and shortcomings are still needed to be further researched and broken by students.
Denitrifying phosphorus removal is mainly performed by a group of facultative anaerobic bacteria capable of simultaneously performing denitrifying and phosphorus removal metabolism, and the metabolic pathways of the bacteria are similar to those of phosphorus accumulating bacteria (PAOs), so that the bacteria are called denitrifying phosphorus accumulating bacteria (DPAOs).
At present, the research on the intrinsic mechanism of denitrifying phosphorus removal is still in the initial stage, and the macroscopically clear denitrifying phosphorus removal process is represented as follows:
under the anaerobic condition, the DPAOs carry out anaerobic phosphorus release as in the traditional phosphorus release process;
under hypoxic conditions, DPAOs react with NO2 -Is an electron receiverThe body oxidizes PHA, a coherent mass within the body, and produces a large amount of energy for assimilation and excessive absorption of inorganic phosphorus in the wastewater, while reducing nitrite nitrogen to nitrogen.
In order to overcome the denitrification and dephosphorization phenomenon, the scholars propose two hypotheses, namely a genus-I theory and a genus-II theory.
The former considers that denitrifying phosphorus removal is completed by phosphorus accumulating bacteria, and the key of whether the phosphorus accumulating bacteria show denitrifying capability is that the phosphorus accumulating bacteria have directional induction or not, so that denitrifying functional enzymes in the phosphorus accumulating bacteria play a role.
The latter considers that two types of phosphorus accumulating bacteria exist, one type is the traditional phosphorus accumulating bacteria, and the aerobic phosphorus removal can be carried out only by taking oxygen as an electron acceptor; another is that either or both of O and O can be substituted2As electron acceptors, and optionally NO2 -Or NO3 -Denitrifying phosphorus accumulating bacteria as electron acceptor.
For both hypotheses, researchers are currently more inclined to the two genera theory.
In addition, researchers have investigated the denitrification phosphorus removal process versus O by comparison2、NO2 -And NO3 -The use of these 3 electron acceptors was found to be NO2 -When the biological nitrogen and phosphorus removal system is used as an electron acceptor, the phosphorus release rate and the phosphorus absorption rate of the biological nitrogen and phosphorus removal system are fastest, and the running period is shortest.
Compared with the traditional phosphorus removal process, the denitrification phosphorus removal process realizes the effect of synchronously removing nitrogen and phosphorus from sewage in a one-carbon dual-purpose mode and a one-bacteria dual-purpose mode, can save about 50 percent of carbon source and 30 percent of oxygen demand, and can also reduce 50 percent of residual sludge.
The endopolymer is a high-energy polymer which is used for transforming and storing organic substances absorbed outside cells in the cells under certain conditions by microorganisms and resisting a starvation environment. In the field of biological treatment of waste water, mention may be made, among others, of mainly Polyhydroxyalkanoates (PHAs), Glycogen (Glycogen) and phosphorus (Poly-P).
PHAs are polymers stored in a granular state in microbial cells, are polymers which are limited in the process of acyl-CoA participating in the tricarboxylic acid cycle (TCA) of microorganisms under the conditions of nutrient imbalance and external carbon source sufficiency, are synthesized from surplus acyl-CoA, mainly comprise Poly- β -hydroxybutyrate (PHB), Poly- β -hydroxyvalerate (PHV) and 3-hydroxy-2-methylvalerate (PH2MV), are ideal carbon source, energy and reducing power storage materials for the microorganisms, and can be oxidized and decomposed under the condition of insufficient external carbon source, and generate a large amount of energy to maintain the basic survival of the microorganisms.
Glycogen is an important component of intracellular polymers and plays an important role in the biological phosphorus removal process. Glycogen is degraded and converted in the polyphosphate accumulating bacteria mainly through two pathways of glycolysis and 2-keto-3-deoxy-6-phosphogluconate, and reduction hydrogen is generated to provide reducing power for the phosphate accumulating bacteria to synthesize PHAs.
Poly-P is also mainly present in the phosphorus accumulating microorganisms, and is degraded under anaerobic conditions to generate energy to assist the phosphorus accumulating microorganisms in completing the process of converting an external carbon source into an internal carbon source.
Many researchers have studied the relevance of the polymer to denitrification and dephosphorization of a sewage biological treatment system.
Researchers research the synchronous nitrification and denitrification phenomenon of PHB as an electron donor, and research results show that PHB can be slowly degraded when an external carbon source is deficient and has the effect of the external carbon source, so that the system has better synchronous nitrification and denitrification performance.
Researches on the synthetic degradation rule of PHAs and glycogen for strengthening the biological phosphorus removal system show that PHAs and glycogen can be used as energy substances for driving denitrifying phosphorus removal. A great deal of research on the inner polymer in the biological nitrogen and phosphorus removal system shows that the synthesis of the inner polymer has a promoting effect on the decontamination performance of the system. However, in the future, how to make microorganisms maximally synthesize the endopolymer and maximally provide energy in the decomposition process, so that the endopolymer becomes a driving force for removing pollutants in sewage is still needed to be further researched and excavated.
Disclosure of Invention
The invention aims to provide a method for treating low-carbon urban sewage by controlling and driving denitrification through an inner polymer, aiming at the problems in the prior art.
The purpose of the invention can be realized by the following technical scheme: a method for treating low-carbon municipal sewage by polymer-driven denitrification comprises the following steps:
s1, inoculation: inoculating flocculent activated sludge into a Sequencing Batch Reactor (SBR), and aerating;
s2, operation regulation: the method comprises a domestication period, an adjustment period and an optimization period, wherein the domestication period comprises a first aerobic stage taking high-COD sewage as inflow water and a second aerobic stage taking low-COD sewage as inflow water.
Preferably, the flocculent activated sludge is taken from a secondary sedimentation tank of a sewage treatment plant.
Preferably, the SBR outer wall is organic glass material, and the main part is 140mm of internal diameter, high 1100 mm's cylindrical, and the bottom is the toper, effective treatment volume 13L. The reactor is filled with water from the top, three sampling ports with different heights are arranged on the outer wall, an anaerobic stirring device is arranged in the reactor, a sludge discharge port is arranged outside the reactor at the bottom, a microporous aeration head is arranged in the reactor at the bottom, air is supplied by an externally connected air compression pump, and the aeration amount is controlled by an LZB-6 glass ion flow meter.
Preferably, the aeration is continuous aeration for 24-30 h.
Through aeration, the flocculent activated sludge is fully absorbed with oxygen and awakened to gradually adapt to the new environment of the reactor, so as to facilitate the later operation regulation and control treatment.
Preferably, in the step of inoculating and operating and regulating, the pH value range of the system in the SBR is controlled to be 7-8, and the temperature is room temperature.
Preferably, the high COD sewage is simulated sewage, the COD value is 1500-2350, the low COD sewage is urban sewage, and the COD value is 160-230.
Preferably, the high COD sewage is simulated sewage, sodium acetate is used as a carbon source, and NH is adopted4Cl is nitrogen source and KH2PO4Is a phosphorus source, the pH value is adjusted by sodium bicarbonate, and trace elements are added for supplement.
The invention uses sodium bicarbonate to adjust the pH and also mainly acts as a buffer solution, so that the pH of the solution in the reactor is basically maintained at neutral and weak alkalinity in the whole operation period.
Preferably, the low COD sewage is urban sewage, the ammonia nitrogen concentration is 25 mg/L-35 mg/L, and the COD/N ratio is 5-10.
Preferably, the water inlet changing amount of the first aerobic stage is 60 to 80 percent, and the water inlet changing amount of the second aerobic stage is 40 to 60 percent
Preferably, the operation mode of the acclimatization period is high-COD sewage inflow (2 min-4 min) → first aerobic stage (60 min-90 min) → low-COD sewage inflow (4 min-6 min) → second aerobic stage (140 min-150 min) → anaerobic stage (120 min-180 min) → precipitation (5 min-10 min) → drainage (5 min-10 min), and the operation time of the acclimatization period is 12 d-16 d.
Preferably, the water inlet in the adjusting period and the water inlet in the optimizing period are urban sewage.
Preferably, the operation mode of the adjustment period is water inflow (4min to 6min) → aerobic phase (200min to 240min) → anaerobic phase (120min to 180min) → precipitation (5min to 10min) → water drainage (5min to 10min), and the operation time of the adjustment period is 18d to 23 d.
Preferably, the operation mode of the optimization period is water inlet (5min) → anaerobic stage (120 min-180 min) → aerobic stage (200 min-240 min) → precipitation (5 min-10 min) → water discharge (5 min-10 min), the aeration amount of the aerobic stage is 1.5L/min-2.5L/min, and the operation time of the optimization period is 30 d-37 d
Preferably, the domestication period, the adjustment period or the optimization period runs for 3-4 periods every day according to the running mode, the rest time is idle, the sludge is discharged quantitatively, and the sludge age is controlled to be 18-22 d.
Preferably, each stage in the operation mode is completed by the timed switching on and off of the SBR automatic control system.
The invention divides the operation regulation step into three stages of acclimatization period, adjustment period and optimization period, and carries out sectional water inflow of high COD sewage and low COD sewage in the acclimatization period, thereby effectively promoting the propagation and growth of heterotrophic microorganism micelle bacteria and leading the aerobic granular sludge generated by culture to be more suitable for the treatment of urban sewage.
The invention adopts high COD sewage in the first aerobic stage of the acclimatization period, the too high carbon source concentration can inhibit the absorption and utilization of the substrate by the microorganism, the carbon source absorption time of the microorganism is prolonged, and the propagation of zoogloea bacteria is easier to promote than filamentous bacteria. K of filamentous fungi according to Monod's equationSAnd mumaxThe value is lower than that of zoogloea bacteria, and the substrate utilization rate of the zoogloea bacteria is higher than that of filamentous bacteria when the substrate concentration is high, so that the zoogloea bacteria can be in an advantage position in growth competition.
The invention adopts the low COD sewage in the second aerobic stage of the acclimatization period, which is closer to the environment of low-carbon urban sewage, and can reduce the adaptation period of aerobic granular sludge in a new environment through the culture of the low COD sewage, thereby more effectively treating the urban sewage. Further, the low COD sewage is directly urban sewage, the components of the urban sewage are more complex than simulated sewage, the urban sewage contains various harmful substances besides nutrient components required by the growth of microorganisms, and the microorganisms in the sludge can secrete more Extracellular Polymers (EPS) to resist external impact when the microorganisms in the sludge do not adapt to the harmful substances in the urban sewage, so that the growth of aerobic granular sludge is promoted. The Extracellular Polymer (EPS) is an important chemical component of the granular sludge, and the main substances of the EPS are polysaccharide, protein, enzyme protein, nucleic acid, phospholipid and humic acid, which can assist cells to stick together, thereby enhancing the formation of aerobic granular sludge and promoting the rapid culture of the aerobic granular sludge.
The adjusting period of the invention aims to enrich denitrifying bacteria and strengthen the removal of nitrogen from aerobic granular sludge. Most of the denitrifying bacteria are facultative anaerobes, and low molecular organic matters can be used as electron donors and NO is used as the electron donors3 -Or NO2 -The denitrification is completed for the final electron acceptor. Under the operation mode of firstly aerobic and then anaerobic in the adjustment period, NH can be generated in a certain time of aerobic period4 +Oxidation of-N to NO3 -N, the macromolecular organic matters are oxidized and decomposed into smaller organic matters, and the oxidation reaction products provide rich nitrogen sources and carbon sources for the reproductive growth of denitrifying bacteria in the anaerobic period so that the denitrifying bacteriaHas more growth competitive advantages. Therefore, the invention is beneficial to the propagation, growth and enrichment of denitrifying bacteria by adopting an aerobic-then-anaerobic operation mode in the adjustment period and controlling the duration of each stage in the operation mode.
The optimization period of the invention aims to promote the aerobic granular sludge to synthesize and accumulate the inner polymer so as to drive the microorganism to remove nitrogen and phosphorus.
According to the invention, an operation mode of anaerobic operation and then aerobic operation is adopted in the optimization period, in an operation period of the optimization period, the anaerobic initial stage after water inflow is a period rich in external carbon sources relative to the whole operation period, and belongs to a period rich in external carbon sources.
The invention adopts the operation mode of anaerobic operation and then aerobic operation in the optimization period, which is beneficial to the growth of the flora with PHAs anabolism function, such as phosphorus-accumulating bacteria, and is beneficial to the enrichment of the microbial flora into the water carbon source to synthesize the inner polymer under the anaerobic condition, and further can metabolize the inner polymer for energy supply in the carbon source shortage period of the aerobic section. So that the aerobic granular sludge subjected to optimized culture has metabolic response of enriching an external carbon source to synthesize an inner polymer and timely decomposing the inner polymer for energy supply.
Meanwhile, the invention also promotes the accumulation/decomposition metabolism of energy storage polymers in heterotrophic microorganisms (zoogloea bacteria) in the aerobic granular sludge through an operation mode of rich-poor carbon source outside the optimization period. In the stage of enriching the external carbon source, the invention removes COD in the urban wastewater by a storage mechanism of heterotrophic microorganisms (zoogloea bacteria), and can greatly reduce the aeration quantity in the COD removal link in the wastewater because the COD is not removed by direct aerobic decomposition. In the following external carbon source depletion phase, heterotrophic microorganisms in the sludge can break down the polymers in the body as their own carbon and energy source.
In the aerobic stage of the optimization stage, the COD concentration is extremely low, the denitrifying bacteria enriched in the adjustment stage can be used as an electron donor to supply denitrifying bacteria to perform a denitrifying process (namely, polymer-driven denitrification) by utilizing the polymers in vivo stored when the carbon source is enriched outside the anaerobic stage of the optimization stage in the subsequent consumption process, and the phosphorus accumulating bacteria enriched in the optimization stage can absorb extracellular phosphorus by utilizing PHA (polyhydroxyalkanoate) as an energy source, so that synchronous nitrogen and phosphorus removal is completed.
The invention adopts the polymer to drive the denitrification, solves the contradiction that the COD in the wastewater needs to be removed before nitrification and the carbon source needs to be supplemented when denitrification is carried out, does not need to add the carbon source subsequently and does not need to carry out high-strength sewage backflow, thereby saving the operation cost and simplifying the operation management. More importantly, the heterotrophic microorganisms can provide a more balanced growth mode for the microorganisms based on a metabolic mechanism of rapid storage and slow consumption of an external carbon source, so that the microorganisms occupy an advantage position in a sewage treatment process and are easily cultured and obtained, and therefore, the bottleneck that the anaerobic ammonia oxidation microorganisms are abnormally sensitive to environmental conditions can be overcome by the polymer-driven denitrification, and the application of the heterotrophic microorganisms in an actual sewage denitrification system is facilitated.
Compared with the prior art, the invention has the following beneficial effects: according to the invention, through alternately feeding high-COD simulated sewage and low-COD urban sewage in the domestication stage, on one hand, filamentous bacteria propagation is inhibited, and zoogloea bacterial propagation is promoted, and on the other hand, a large amount of EPS is generated by utilizing the reaction of microorganisms to external environment stimulation, so that the rapid culture of aerobic granular sludge is promoted; by controlling the operation mode of the adjustment period, denitrifying bacteria are effectively enriched, and the removal of nitrogen by aerobic granular sludge is enhanced; and the control of the operation mode in the optimization period promotes the synthesis and accumulation of the endopolymer by the aerobic granular sludge, thereby driving the proceeding of the denitrification and the dephosphorization of the microorganism.
Detailed Description
The following are specific examples of the present invention and further describe the technical solutions of the present invention, but the present invention is not limited to these examples.
Example 1
The method for treating low-carbon urban sewage by utilizing the depolymerization driven by the inner polymer comprises the following steps:
(1) inoculation: flocculent activated sludge taken from a secondary sedimentation tank of a sewage treatment plant is inoculated into a Sequencing Batch Reactor (SBR) and continuously aerated for 26 hours, the outer wall of the SBR is made of organic glass, a main body is cylindrical with the inner diameter of 140mm and the height of 1100mm, the bottom of the SBR is conical, and the effective treatment volume is 13L. The reactor is filled with water from the top, three sampling ports with different heights are arranged on the outer wall, an anaerobic stirring device is arranged in the reactor, a sludge discharge port is arranged outside the reactor at the bottom, a microporous aeration head is arranged in the reactor at the bottom, air is supplied by an externally connected air compression pump, and the aeration amount is controlled by an LZB-6 glass ion flow meter.
(2) Operation regulation and control: comprises an acclimatization period, an adjustment period and an optimization period;
wherein the domestication period also comprises a first aerobic stage and a second aerobic stage taking the low COD sewage as the inlet water; in the first aerobic stage, high COD sewage is used as inlet water, high COD sewage is used as simulated sewage, COD value is 2000, sodium acetate is used as carbon source, NH is added4Cl is nitrogen source and KH2PO4As a phosphorus source, adjusting the pH value by sodium bicarbonate, and adding trace elements for supplement, wherein the water change amount of the inlet water in the first aerobic stage is 70 percent; the low COD sewage in the second aerobic stage is urban sewage, the COD value is 190, the ammonia nitrogen concentration is 30mg/L, the COD/N ratio is 6.3, and the water inlet changing amount in the second aerobic stage is 40-60 percent;
the operation mode of the whole acclimation period is high COD sewage inflow (3min) → first aerobic stage (75min) → low COD sewage inflow (5min) → second aerobic stage (145min) → anaerobic stage (150min) → precipitation (8min) → drainage (7min), and the operation time is 14 d.
The water inlet in the adjusting period and the water inlet in the optimizing period are urban sewage which is the same as the urban sewage in the second aerobic stage in the acclimatization period;
the operation mode of the adjustment period is water inlet (5min) → aerobic period (220min) → anaerobic period (150min) → precipitation (8min) → water discharge (7min), and the operation time is 20 d.
The operation mode of the optimization period is water inlet (5min) → anaerobic stage (150min) → aerobic stage (220min) → precipitation (8min) → water discharge (7min), the aeration amount of the aerobic stage is 2L/min, and the operation time is 35 d.
The acclimatization period, the adjustment period and the optimization period of the operation regulation and control are operated for 3 periods every day according to the operation mode, the rest time is idle, the sludge is discharged quantitatively, and the sludge age is controlled to be 20 days. And each stage in the operation mode is completed by the timing switching on and off of the SBR automatic control system.
And the pH value range of the system in the SBR is controlled to be 7 during the inoculation and operation regulation and control, and the temperature is room temperature.
Example 2
The method for treating low-carbon urban sewage by utilizing the depolymerization driven by the inner polymer comprises the following steps:
(1) inoculation: flocculent activated sludge taken from a secondary sedimentation tank of a sewage treatment plant is inoculated into a Sequencing Batch Reactor (SBR) and continuously aerated for 24 hours, the outer wall of the SBR is made of organic glass, a main body is cylindrical with the inner diameter of 140mm and the height of 1100mm, the bottom of the SBR is conical, and the effective treatment volume is 13L. The reactor is filled with water from the top, three sampling ports with different heights are arranged on the outer wall, an anaerobic stirring device is arranged in the reactor, a sludge discharge port is arranged outside the reactor at the bottom, a microporous aeration head is arranged in the reactor at the bottom, air is supplied by an externally connected air compression pump, and the aeration amount is controlled by an LZB-6 glass ion flow meter.
(2) Operation regulation and control: comprises an acclimatization period, an adjustment period and an optimization period;
wherein the domestication period also comprises a first aerobic stage and a second aerobic stage taking the low COD sewage as the inlet water; in the first aerobic stage, high COD sewage is used as inlet water, high COD sewage is used as simulated sewage, COD value is 2350, sodium acetate is used as carbon source, and NH is used as carbon source4Cl is nitrogen source and KH2PO4As a phosphorus source, adjusting the pH value by sodium bicarbonate, and adding trace elements for supplement, wherein the water change amount of the inlet water in the first aerobic stage is 60 percent; the low COD sewage in the second aerobic stage is urban sewage, the COD value is 230, the ammonia nitrogen concentration is 25mg/L, the COD/N ratio is 9.2, and the water inlet exchange amount in the second aerobic stage is 40 percent;
the operation mode of the whole acclimation period is high COD sewage inflow (3min) → first aerobic stage (60min) → low COD sewage inflow (5min) → second aerobic stage (150min) → anaerobic stage (180min) → precipitation (10min) → drainage (5min), and the operation time is 12 d.
The water inflow in the adjusting period and the optimizing period is urban sewage;
the operation mode of the adjustment period is water inlet (5min) → aerobic period (200min) → anaerobic period (180min) → precipitation (5min) → water discharge (5min), and the operation time is 23 d.
The operation mode of the optimization period is water inlet (5min) → anaerobic stage (180min) → aerobic stage (200min) → precipitation (5min) → water discharge (5min), the aeration amount of the aerobic stage is 2L/min, and the operation time is 30 d.
The acclimatization period, the adjustment period and the optimization period of the operation regulation and control are operated for 3 periods every day according to the operation mode, the rest time is idle, the sludge is discharged quantitatively, and the sludge age is controlled to be 18 d. And each stage in the operation mode is completed by the timing switching on and off of the SBR automatic control system.
And the pH value range of the system in the SBR is controlled to be 7 during the inoculation and operation regulation and control, and the temperature is room temperature.
Example 3
The method for treating low-carbon urban sewage by utilizing the depolymerization driven by the inner polymer comprises the following steps:
(1) inoculation: the flocculent activated sludge taken from a secondary sedimentation tank of a sewage treatment plant is inoculated into a Sequencing Batch Reactor (SBR) and continuously aerated for 30 hours, the outer wall of the SBR is made of organic glass, the main body is cylindrical with the inner diameter of 140mm and the height of 1100mm, the bottom of the SBR is conical, and the effective treatment volume is 13L. The reactor is filled with water from the top, three sampling ports with different heights are arranged on the outer wall, an anaerobic stirring device is arranged in the reactor, a sludge discharge port is arranged outside the reactor at the bottom, a microporous aeration head is arranged in the reactor at the bottom, air is supplied by an externally connected air compression pump, and the aeration amount is controlled by an LZB-6 glass ion flow meter.
(2) Operation regulation and control: comprises an acclimatization period, an adjustment period and an optimization period;
wherein the domestication period also comprises a first aerobic stage and a second aerobic stage taking the low COD sewage as the inlet water; in the first aerobic stage, high COD sewage is used as inlet water, high COD sewage is used as simulated sewage, COD value is 1500, sodium acetate is used as carbon source, NH is added4Cl is nitrogen source and KH2PO4As a phosphorus source, adjusting the pH value by sodium bicarbonate, and adding trace elements for supplement, wherein the water change amount of the inlet water in the first aerobic stage is 80 percent; the low COD sewage in the second aerobic stage is urban sewage, the COD value is 160, the ammonia nitrogen concentration is 35mg/L, the COD/N ratio is 4.6, and the water inlet exchange amount in the second aerobic stage is 60 percent;
the operation mode of the whole acclimation period is high COD sewage inflow (3min) → first aerobic stage (90min) → low COD sewage inflow (5min) → second aerobic stage (140min) → anaerobic stage (120min) → precipitation (5min) → drainage (10min), and the operation time is 16 d.
The water inflow in the adjusting period and the optimizing period is urban sewage;
the operation mode of the adjustment period is water inlet (5min) → aerobic period (240min) → anaerobic period (120min) → precipitation (10min) → water discharge (10min), and the operation time is 18 d.
The operation mode of the optimization period is water inlet (5min) → anaerobic stage (120min) → aerobic stage (240min) → precipitation (10min) → water discharge (10min), the aeration rate of the aerobic stage is 2L/min, and the operation time is 37d
The acclimatization period, the adjustment period and the optimization period of the operation regulation and control are operated for 3 periods every day according to the operation mode, the rest time is idle, the sludge is discharged quantitatively, and the sludge age is controlled to be 22 d. And each stage in the operation mode is completed by the timing switching on and off of the SBR automatic control system.
And the pH value range of the system in the SBR is controlled to be 7 during the inoculation and operation regulation and control, and the temperature is room temperature.
Comparative example 1
In the acclimation period, the operation mode was the same as that of example 1 except that the aerobic stage had only the first aerobic stage in which high COD wastewater was used as the influent water and had no second aerobic stage in which low COD wastewater was used as the influent water.
Comparative example 2
In the operation mode of the optimization period, the aerobic stage is performed first, and then the anaerobic stage is performed, that is, the original anaerobic stage and the aerobic stage are exchanged, and the rest is the same as that in the embodiment 1.
The drainage in the later period of the optimization period and the later stable operation period in the examples 1 to 3 and the comparative examples 1 to 2 of the invention is detected, and the pollutant removal rate is calculated, and the result is shown in table 1.
Table 1: removal rate of contaminants in wastewater during drainage period in examples 1 to 3 and comparative examples 1 to 2
Figure BDA0002254335820000141
As can be seen from Table 1, the removal rate of pollutants from municipal wastewater can reach a high level at the late stage of the optimization period and at the stable operation period thereafter by using the method of the present invention.
The technical scope of the invention claimed by the embodiments of the present application is not exhaustive, and new technical solutions formed by equivalent replacement of single or multiple technical features in the technical solutions of the embodiments are also within the scope of the invention claimed by the present application; in all the embodiments of the present invention, which are listed or not listed, each parameter in the same embodiment only represents an example (i.e., a feasible embodiment) of the technical solution, and there is no strict matching and limiting relationship between the parameters, wherein the parameters may be replaced with each other without departing from the axiom and the requirements of the present invention, unless otherwise specified.
The technical means disclosed by the scheme of the invention are not limited to the technical means disclosed by the technical means, and the technical scheme also comprises the technical scheme formed by any combination of the technical characteristics. While the foregoing is directed to embodiments of the present invention, it will be appreciated by those skilled in the art that various changes may be made in the embodiments without departing from the principles of the invention, and that such changes and modifications are intended to be included within the scope of the invention.

Claims (6)

1. A method for treating low-carbon municipal sewage by polymer-driven denitrification is characterized by comprising the following steps:
s1, inoculation: inoculating flocculent activated sludge into a Sequencing Batch Reactor (SBR), and aerating;
s2, operation regulation: the method comprises a domestication period, an adjustment period and an optimization period, wherein the domestication period comprises a first aerobic stage taking high-COD sewage as inflow water and a second aerobic stage taking low-COD sewage as inflow water.
2. The method according to claim 1, wherein the high COD sewage is simulated sewage with a COD value of 1500-2350, and the low COD sewage is municipal sewage with a COD value of 160-230.
3. The method according to claim 1, wherein the operation mode of the acclimatization period is high COD sewage inflow (2 min-4 min) → first aerobic stage (60 min-90 min) → low COD sewage inflow (4 min-6 min) → second aerobic stage (140 min-150 min) → anaerobic stage (120 min-180 min) → precipitation (5 min-10 min) → drainage (5 min-10 min), and the operation time of the acclimatization period is 12 d-16 d.
4. The method of claim 1, wherein the feed water during the conditioning period and the optimization period is municipal sewage.
5. The method according to claim 4, wherein the operation mode of the adjustment period is water inflow (4 min-6 min) → aerobic period (200 min-240 min) → anaerobic period (120 min-180 min) → precipitation (5 min-10 min) → water drainage (5 min-10 min), and the operation time of the adjustment period is 18 d-23 d.
6. The method according to claim 4, wherein the operation mode of the optimization period is water inflow (4 min-6 min) → anaerobic phase (120 min-180 min) → aerobic phase (200 min-240 min) → precipitation (5 min-10 min) → water drainage (5 min-10 min), the aeration amount of the aerobic phase is 1.5L/min-2L/min, and the operation time of the optimization period is 30 d-37 d.
CN201911046799.7A 2019-10-30 2019-10-30 Method for treating low-carbon urban sewage by polymer-driven denitrification Active CN110845001B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201911046799.7A CN110845001B (en) 2019-10-30 2019-10-30 Method for treating low-carbon urban sewage by polymer-driven denitrification

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201911046799.7A CN110845001B (en) 2019-10-30 2019-10-30 Method for treating low-carbon urban sewage by polymer-driven denitrification

Publications (2)

Publication Number Publication Date
CN110845001A true CN110845001A (en) 2020-02-28
CN110845001B CN110845001B (en) 2022-03-15

Family

ID=69599195

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201911046799.7A Active CN110845001B (en) 2019-10-30 2019-10-30 Method for treating low-carbon urban sewage by polymer-driven denitrification

Country Status (1)

Country Link
CN (1) CN110845001B (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111596021A (en) * 2020-05-26 2020-08-28 同济大学 Water body carbon source quality evaluation method, equipment and device and readable storage medium
CN111875046A (en) * 2020-06-29 2020-11-03 同济大学 Culture medium and domestication method for directionally enriching nitrite nitrogen accumulation type denitrifying bacteria from activated sludge
CN114684924A (en) * 2020-12-30 2022-07-01 中国石油化工股份有限公司 Denitrification treatment method for salt-containing wastewater
CN115611416A (en) * 2022-10-11 2023-01-17 哈尔滨工业大学 Method for rapidly enriching aerobic denitrifying bacteria based on PHA (polyhydroxyalkanoate) synthesis process by mixed flora
CN116562412A (en) * 2022-11-16 2023-08-08 广州市净水有限公司 Low-carbon operation optimization method for sewage biological treatment

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080053897A1 (en) * 2000-10-02 2008-03-06 Jingxu Zhu Liquid-solid fluidized bed waste water treatment system for simultaneous carbon, nitrogen and phosphorous removal
CN102010058A (en) * 2010-09-29 2011-04-13 北京师范大学 Aerobic granular sludge culture method suitable to low-concentration domestic sewage
CN103601341A (en) * 2013-11-22 2014-02-26 北京工业大学 SBR (Sequencing Batch Reactor) and SBBR (Sequencing Biofilm Batch Reactor) municipal sewage high-efficiency biological treatment method and device based on anaerobic ammonia oxidation
CN105174452A (en) * 2015-07-19 2015-12-23 北京工业大学 Apparatus for reinforcing nitrogen and phosphorus removal coupling and sludge reduction of low C/N ratio municipal domestic sewage by using carbon source in sludge, and method thereof
CN108117158A (en) * 2016-11-29 2018-06-05 中国石油化工股份有限公司 A kind of fast culture process of nitrogen removal granule sludge

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080053897A1 (en) * 2000-10-02 2008-03-06 Jingxu Zhu Liquid-solid fluidized bed waste water treatment system for simultaneous carbon, nitrogen and phosphorous removal
CN102010058A (en) * 2010-09-29 2011-04-13 北京师范大学 Aerobic granular sludge culture method suitable to low-concentration domestic sewage
CN103601341A (en) * 2013-11-22 2014-02-26 北京工业大学 SBR (Sequencing Batch Reactor) and SBBR (Sequencing Biofilm Batch Reactor) municipal sewage high-efficiency biological treatment method and device based on anaerobic ammonia oxidation
CN105174452A (en) * 2015-07-19 2015-12-23 北京工业大学 Apparatus for reinforcing nitrogen and phosphorus removal coupling and sludge reduction of low C/N ratio municipal domestic sewage by using carbon source in sludge, and method thereof
CN108117158A (en) * 2016-11-29 2018-06-05 中国石油化工股份有限公司 A kind of fast culture process of nitrogen removal granule sludge

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
杨国靖: "单级SBR系统好氧颗粒污泥实现同步除磷脱氮及其微生物机理的研究", 《中国优秀博硕士学位论文全文数据库(硕士) 工程科技Ⅰ辑》 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111596021A (en) * 2020-05-26 2020-08-28 同济大学 Water body carbon source quality evaluation method, equipment and device and readable storage medium
CN111596021B (en) * 2020-05-26 2022-02-11 同济大学 Water body carbon source quality evaluation method, equipment and device and readable storage medium
CN111875046A (en) * 2020-06-29 2020-11-03 同济大学 Culture medium and domestication method for directionally enriching nitrite nitrogen accumulation type denitrifying bacteria from activated sludge
CN114684924A (en) * 2020-12-30 2022-07-01 中国石油化工股份有限公司 Denitrification treatment method for salt-containing wastewater
CN115611416A (en) * 2022-10-11 2023-01-17 哈尔滨工业大学 Method for rapidly enriching aerobic denitrifying bacteria based on PHA (polyhydroxyalkanoate) synthesis process by mixed flora
CN116562412A (en) * 2022-11-16 2023-08-08 广州市净水有限公司 Low-carbon operation optimization method for sewage biological treatment
CN116562412B (en) * 2022-11-16 2024-02-20 广州市净水有限公司 Low-carbon operation optimization method for sewage biological treatment

Also Published As

Publication number Publication date
CN110845001B (en) 2022-03-15

Similar Documents

Publication Publication Date Title
CN110845001B (en) Method for treating low-carbon urban sewage by polymer-driven denitrification
CN112158952B (en) Device and method for treating low-carbon-nitrogen-ratio wastewater through continuous flow AOA (argon oxygen decarburization) shortcut nitrification and anaerobic ammonia oxidation coupling sludge fermentation denitrification
CN104986923B (en) A kind of apparatus and method of the multistage A/O biological denitrificaions based on municipal sewage short distance nitration Anammox
CN101985376B (en) Method for removing carbon and nitrogen pollutants in waste water in one step
CN113998783B (en) Low-carbon nitrogen and phosphorus removal device and method for municipal sewage based on partial return sludge deep anaerobic treatment
CN113087134A (en) Device and method for quickly realizing integration of partial shortcut nitrification/anaerobic ammonia oxidation by adding hydroxylamine and combining with low sludge age control
CN101538104B (en) Improved A2N denitrifying dephosphatation and deep denitrification device and method
CN106186323A (en) A kind of method starting integration short distance nitration anaerobic ammonia oxidation process
CN110330180A (en) A kind of system and method for mainstream short-cut nitrification and denitrification/Anammox
CN108658229B (en) Device and method for controlling autotrophic/heterotrophic deep denitrification process
CN112607861A (en) Process for treating municipal sewage by using excess sludge fermentation carbon source to strengthen denitrification dephosphorization, partial short-cut denitrification and anaerobic ammonia oxidation
CN113233593A (en) Sewage treatment process and sewage treatment device
CN114230006A (en) Natural enrichment method for anaerobic ammonium oxidation bacteria
CN110171904B (en) Continuous flow AAO dephosphorization and partial denitrification series-connection combined type fixed biological membrane activated sludge autotrophic denitrification device and method
CN114477448A (en) Intelligent integrated advanced denitrification sewage treatment system based on SND (selective non-catalytic reduction) process
CN113562834A (en) Return sludge recovery and reduction system and method
CN110054296B (en) A/O/A SBR process for treating low C/N ratio municipal sewage
CN115403150B (en) Device and method for treating low-carbon-nitrogen-ratio urban sewage by using IFAS-SBR
CN201538728U (en) Improved A2N denitrifying dephosphatation and deep denitrification device
CN115124139B (en) Starting method of sulfur autotrophic denitrification system in low-temperature environment
CN107129046B (en) A2Improved process for water treatment of/O-BCO
CN1597566A (en) Sewage treatment method using sequential batch type reactor
CN103011409B (en) Method for realizing stable operation of nitrosification of domestic sewage in sequencing batch reactor (SBR) by using intermittent aeration
CN112299561A (en) Landfill leachate short-cut nitrification and denitrification treatment method
CN117003390B (en) Three-reflux multistage A/O device and method for strengthening endogenous synchronous denitrification and dephosphorization sludge

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant