CN110840396A - Patterned microarray of odor molecules, preparation method and application thereof - Google Patents
Patterned microarray of odor molecules, preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to a printing technology, in particular to a patterned microarray of odor molecules and a preparation method and application thereof. The patterned microarray comprises a substrate and a core-shell structure formed on the substrate, wherein the core-shell structure takes odor molecules as a core and takes poly (lactic acid-co-glycolic acid) as a shell. The preparation method comprises the following steps: printing a suspension containing scent molecules on a substrate in a predetermined pattern; printing the poly (lactic-co-glycolic acid) solution on the suspension of the odor molecules on the substrate in situ, and obtaining the patterned microarray with the core-shell structure after the solvent in the poly (lactic-co-glycolic acid) solution is volatilized. The effective odor release characteristics of the patterned microarray of odorant molecules provided by the present invention make it promising for odor on-demand release, research of olfactory sensitivity and enhancement of olfactory exercise. In addition, the patterned microarray of the odor molecules provided by the invention has the characteristic of high stability, and can not deform after being stored in an atmospheric environment for a long time.
Description
Technical Field
The invention relates to a printing technology, in particular to a patterned microarray of odor molecules and a preparation method and application thereof.
Background
Olfaction is a perception sensed by an organ. Humans also have an unusually sensitive sense of smell and unknowingly have a large impact on our lives. Subtle odors can affect mood, behavior, and decision making without being perceived by us. The sense of smell decreases with age and the occurrence of olfactory disorders. Among them, olfactory sensitivity is a major indicator for evaluating certain neurodegenerative diseases and can be detected by an odor test, but olfactory performance is greatly affected by the kind of odor tested.
Olfactory degeneration requires treatment and measures can be taken to improve and maintain the sense of smell in daily life. One of the measures taken is to delay degeneration by constantly "exercising" the nose receptors and constantly stimulating the olfactory nerves. Therefore, a plurality of types of products capable of quantitatively releasing odor have potential application value in the aspect of olfactory sensitivity detection.
Disclosure of Invention
The object of the present invention is to provide a product which allows the quantitative release of the smell, thus allowing an effective detection or "training" of the olfactory sensitivity.
In order to achieve the above objects, the present invention provides, in one aspect, a patterned microarray of odor molecules, including a substrate and a core-shell structure formed on the substrate, the core-shell structure having odor molecules as a core and poly (lactic-co-glycolic acid) as a shell.
In a second aspect, the present invention provides a method of preparing a patterned microarray of odorant molecules, the method comprising:
(1) printing a suspension containing scent molecules on a substrate in a predetermined pattern;
(2) printing a poly (lactic-co-glycolic acid) solution in situ on the suspension of the odor molecules printed on the substrate, and volatilizing the solvent in the poly (lactic-co-glycolic acid) solution to obtain the patterned microarray having a core-shell structure with the odor molecules as cores and the poly (lactic-co-glycolic acid) as shells arranged according to a predetermined pattern.
In a third aspect, the present invention provides a patterned microarray prepared by the method described above.
In a fourth aspect, the present invention provides the use of a patterned microarray as described above in the manufacture of a product for scent on demand release, olfactory sensitivity detection and olfactory sensitivity exercise.
The ink-jet printing technology is used as a technical means capable of accurately distributing micro-nano materials in space and function, and the unique property of the ink-jet printing technology is reflected in the aspect of realizing the integration of materials and functions. The technology carries out direct patterning deposition on various types of specific functional materials in a non-contact mode, has the advantages of simple and convenient method, low cost, flexibility, rapidness and the like, becomes one of the most promising patterning methods, and is widely concerned in the fields of functional device research and popularization and application. In recent years, the inkjet printing technology can realize large-area direct writing of microstructures and functional patterns, and is widely applied to patterning of biological functional materials. The inventor of the invention develops the application of ink-jet printing by starting from the existing technology of preparing a microstructure by ink-jet printing, and provides a simple, efficient, green and environment-friendly odor patterning technology capable of being prepared in a large area, so that a core-shell structure (taking odor molecules as cores and poly (lactic-co-glycolic acid) (PLGA) as shells) microarray containing odor is manufactured and used for programmable release of various perfumes. After scratching the protective PLGA shells, a quantitative concentration of the odor molecules can be released from the microarray-based chip (the concentration of odor release can be controlled by scratching different numbers of shell layers). By arranging the scent molecule core layer as one or more scents, the release of multiple mixed scents can be achieved. The effective odor release characteristics of the patterned microarray of odorant molecules provided by the present invention make it promising for odor on-demand release, research of olfactory sensitivity and enhancement of olfactory exercise.
In addition, the patterned microarray of the odor molecules provided by the invention has the characteristic of high stability, and can not deform after being stored in an atmospheric environment for a long time.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention.
FIG. 1 is a schematic representation of a patterned microarray of scent molecules produced by ink jet printing according to the present invention.
FIG. 2 is a detailed view of a patterned microarray of scent molecules prepared by ink-jet printing in example 1 of the present invention.
FIG. 3 is a diagram of a patterned microarray of three odor molecules of longan, milk and vanilla prepared by inkjet printing in examples 1-3 of the present invention.
Fig. 4 is a diagram of a patterned microarray of scent molecules in multiple patterns prepared by inkjet printing in examples 4 and 5 of the present invention.
Fig. 5 is a diagram of a patterned microarray of odor molecules prepared by inkjet printing on the surfaces of a glass sheet, a silicon wafer (hard material), and a PET film (flexible material) in example 6 of the present invention.
FIG. 6 is a diagram of a patterned microarray of odor molecules of ammonia odor prepared by ink-jet printing in example 7 of the present invention, and the quantitative release of the odor molecules was detected by precision test paper with pH 8.0-10.0.
FIG. 7 shows the KMnO samples of the products prepared in the examples4Stability in solution test results.
Detailed Description
The endpoints of the ranges and any values disclosed herein are not limited to the precise range or value, and such ranges or values should be understood to encompass values close to those ranges or values. For ranges of values, between the endpoints of each of the ranges and the individual points, and between the individual points may be combined with each other to give one or more new ranges of values, and these ranges of values should be considered as specifically disclosed herein.
In a first aspect, the present invention provides a patterned microarray of odor molecules, comprising a substrate and a core-shell structure formed on the substrate, the core-shell structure having odor molecules as a core and poly (lactic-co-glycolic acid) as a shell.
According to the invention, in the core of the core-shell structure, in addition to the odour molecules, a carrier for the odour molecules can be present, for example a solvent in which the odour molecules are dissolved, and an auxiliary agent which helps to disperse the odour molecules uniformly in the solvent. According to a preferred embodiment of the invention, the odor molecules are present in the form of a suspension of odor molecules, which suspension contains odor molecules, emulsifiers, alcohols and solvents. The amount of the components in the suspension can be selected within wide limits as long as the odor molecules are stably and homogeneously present in the suspension, preferably, in the odor molecule-containing suspension, the odor molecules are present in an amount of 0.01 to 50 wt.%, the alcohol is present in an amount of 2 to 3 wt.%, and the emulsifier is present in an amount of 0.25 to 5 wt.%, preferably 2 to 4.5 wt.%.
Preferably, the emulsifier is polyvinyl alcohol.
Preferably, the alcohol is ethylene glycol.
Preferably, the solvent is water.
According to a preferred embodiment of the invention, the suspension contains odor molecules, polyvinyl alcohol, ethylene glycol and water, wherein the content of the odor molecules is 0.01 to 50 wt.%, the content of the ethylene glycol is 2 to 3 wt.%, and the content of the polyvinyl alcohol is 0.25 to 5 wt.%. In the preferred case, the prepared core-shell structure is more stable to store in the atmospheric environment, and the release of the odor molecules is more stable and easier to control.
According to the present invention, the type of the odorant is not limited, and may be any odorous substance, such as, but not limited to, alcohol, aldehyde, ester, protein, etc., and more specifically, may be various essences, such as, but not limited to, one or more of milk essence, longan essence, vanilla essence, cola essence, apple essence, cumin essence, lemon essence, walnut essence, grape essence, almond essence, rose essence, and ammonia water. The skilled person can select the desired one according to the actual need. In addition, the core-shell structure of the invention may contain a single odor molecule, or different odor molecules, where the different odor molecules may be different odor molecules mixed in one core-shell structure, or multiple single odor molecules may be respectively arranged in different core-shell structures, so as to release different odors according to requirements.
According to the invention, the substrate can be various substrates, can be a hard substrate or a flexible substrate, and the hard substrate can be but is not limited to one of a silicon chip, a glass sheet, a quartz sheet, an iron sheet, a copper sheet, an aluminum sheet and an aluminum oxide sheet; the flexible substrate may be, but is not limited to, one of a PDMS film, a PET film, a PS film, a PU film, and a PI film.
According to the invention, the shape of the core-shell structure may vary in particular depending on the surface tension of the suspension and the type of substrate, generally hemispherical. Preferably, the particle size of the core-shell structure is 0.5-1.5 mm.
According to the present invention, it is preferable that, in the core-shell structure, the particle diameter of the core layer is 0.8 to 1.0mm, and the thickness of the shell layer is 0.10 to 0.15 mm.
According to the present invention, the pattern in the patterned microarray can be any pattern, and can be designed according to specific situations and actual preferences. According to a specific embodiment of the present invention, the pattern is a lattice pattern of a core-shell structure, and preferably, the lattice pattern is a periodic single-point array. More preferably, the distance between the central connecting lines of two adjacent core-shell structures is 0.5-1.5 cm.
According to the present invention, the method for preparing the patterned microarray is not particularly limited, and for example, a fine needle may be used to uniformly spread a suspension containing the odor molecule on a substrate, and then a solution containing poly (lactic-co-glycolic acid) may be used to wrap the spread suspension liquid, and the core-shell structure may be obtained after the solution containing poly (lactic-co-glycolic acid) is dried.
The solvent in the solution containing poly (lactic acid-co-glycolic acid) may be any solvent capable of dissolving the solution containing poly (lactic acid-co-glycolic acid), but the solvent is preferably a solvent that is easily volatilized because of the subsequent drying procedure. According to a preferred embodiment of the invention, the solvent is dichloromethane.
According to the present invention, in the solution containing poly (lactic acid-co-glycolic acid), the concentration of the poly (lactic acid-co-glycolic acid) is not particularly limited, and in view of the stability of the core-shell structure to be formed, the concentration of the poly (lactic acid-co-glycolic acid) is preferably 2 to 3% by weight.
According to a preferred embodiment of the invention, the patterned microarray of scent molecules is prepared by printing techniques. Thus, in a second aspect, as shown in fig. 1, the present invention provides a method of preparing a patterned microarray of odorant molecules, the method comprising:
(1) printing a suspension containing scent molecules on a substrate in a predetermined pattern;
(2) printing a poly (lactic-co-glycolic acid) solution in situ on the suspension of the odor molecules printed on the substrate, and volatilizing the solvent in the poly (lactic-co-glycolic acid) solution to obtain the patterned microarray having a core-shell structure with the odor molecules as cores and the poly (lactic-co-glycolic acid) as shells arranged according to a predetermined pattern.
According to the invention, the suspension may contain, in addition to the odour molecules, a carrier for the odour molecules, for example a solvent in which the odour molecules are dissolved and an adjuvant which helps to disperse the odour molecules uniformly in the solvent. According to a preferred embodiment of the invention, the odor molecules are present in the form of a suspension of odor molecules, which suspension contains odor molecules, emulsifiers, alcohols and solvents. The amount of the components in the suspension can be selected within wide limits as long as the odor molecules are stably and homogeneously present in the suspension, preferably, in the odor molecule-containing suspension, the odor molecules are present in an amount of 0.01 to 50 wt.%, the alcohol is present in an amount of 2 to 3 wt.%, and the emulsifier is present in an amount of 0.25 to 5 wt.%, preferably 2 to 4.5 wt.%.
Preferably, the emulsifier is polyvinyl alcohol.
Preferably, the alcohol is ethylene glycol.
Preferably, the solvent is water.
According to a preferred embodiment of the invention, the suspension contains odor molecules, polyvinyl alcohol, ethylene glycol and water, wherein the content of the odor molecules is 0.01 to 50 wt.%, the content of the ethylene glycol is 2 to 3 wt.%, and the content of the polyvinyl alcohol is 0.25 to 5 wt.%. In the preferred case, the prepared core-shell structure is more stable to store in the atmospheric environment, and the release of the odor molecules is more stable and easier to control.
According to the present invention, the type of the odorant is not limited, and may be any odorous substance, such as, but not limited to, alcohol, aldehyde, ester, protein, etc., and more specifically, may be various essences, such as, but not limited to, one or more of milk essence, longan essence, vanilla essence, cola essence, apple essence, cumin essence, lemon essence, walnut essence, grape essence, almond essence, rose essence, and ammonia water. The skilled person can select the desired one according to the actual need. In addition, the core-shell structure of the invention may contain a single odor molecule, or different odor molecules, where the different odor molecules may be different odor molecules mixed in one core-shell structure, or multiple single odor molecules may be respectively arranged in different core-shell structures, so as to release different odors according to requirements.
According to the invention, the substrate can be various substrates, can be a hard substrate or a flexible substrate, and the hard substrate can be but is not limited to one of a silicon chip, a glass sheet, a quartz sheet, an iron sheet, a copper sheet, an aluminum sheet and an aluminum oxide sheet; the flexible substrate may be, but is not limited to, one of a PDMS film, a PET film, a PS film, a PU film, and a PI film.
According to the invention, the shape of the core-shell structure may vary in particular depending on the surface tension of the suspension and the type of substrate, generally hemispherical. Preferably, the particle size of the core-shell structure is 0.5-1.5 mm.
According to the present invention, it is preferable that, in the core-shell structure, the particle diameter of the core layer is 0.8 to 1.0mm, and the thickness of the shell layer is 0.10 to 0.15 mm.
According to the present invention, the pattern in the patterned microarray can be any pattern, and can be designed according to specific situations and actual preferences. According to a specific embodiment of the present invention, the pattern is a lattice pattern of a core-shell structure, and preferably, the lattice pattern is a periodic single-point array. More preferably, the distance between the central connecting lines of two adjacent core-shell structures is 0.5-1.5 cm.
The solvent in the solution containing poly (lactic acid-co-glycolic acid) may be any solvent capable of dissolving the solution containing poly (lactic acid-co-glycolic acid), but the solvent is preferably a solvent that is easily volatilized because of the subsequent drying procedure. According to a preferred embodiment of the invention, the solvent is dichloromethane.
According to the present invention, in the solution containing poly (lactic acid-co-glycolic acid), the concentration of the poly (lactic acid-co-glycolic acid) is not particularly limited, and in view of the stability of the core-shell structure to be formed, the concentration of the poly (lactic acid-co-glycolic acid) is preferably 2 to 3% by weight.
According to the invention, the printing is preferably ink-jet printing. Specifically, the suspension containing the odor molecules is filled into an ink box of an ink-jet printer as ink, a printing nozzle with a proper inner diameter (for example, 0.06mm) is selected to print according to a preset pattern, then the poly (lactic-co-glycolic acid) solution is filled into the ink box of the ink-jet printer, in-situ printing is carried out according to the preset pattern, the poly (lactic-co-glycolic acid) solution is laid on the odor molecule ink drop, and after the solvent in the poly (lactic-co-glycolic acid) solution is volatilized to separate out PLGA solid, the core-shell structure can be obtained.
Wherein the term "in situ printing" is to use the same pattern, overlaying poly (lactic-co-glycolic acid) on the odor molecule suspension pattern to form a core-shell protective structure.
In a third aspect, the present invention provides a patterned microarray prepared by the method described above.
In a fourth aspect, the present invention provides the use of a patterned microarray as described above in the manufacture of a product for scent on demand release, olfactory sensitivity detection and olfactory sensitivity exercise.
The present invention will be described in detail below by way of examples.
Example 1
This example illustrates a patterned microarray of odor molecules and preparation thereof
Dissolving 5g of longan essence solution (water-based spice), 2.5g of ethylene glycol and 4.5g of polyvinyl alcohol (PVA, 10 wt%) in 100g of water, uniformly mixing to obtain a suspension containing odor molecules, filling the suspension into an ink box of an ink-jet printer after preparation, selecting a printing nozzle with the inner diameter of 0.06mm, and printing a 9 x 4 dot matrix liquid drop pattern on a PET film substrate according to a preset pattern. Poly (lactic-co-glycolic acid) (PLGA, 2.5 wt%, solvent is dichloromethane) is loaded into an ink box of an ink-jet printer, a printing nozzle with the inner diameter of 0.06mm is selected, in-situ printing is carried out according to a preset pattern, the printing nozzle is covered on perfume molecular ink drops, and PLGA solid is separated out after the dichloromethane is volatilized, so that a core-shell structure (the core-shell structure is hemispherical with the particle size of 0.9-1.15mm, in the core-shell structure, the particle size of a core layer is 0.8-1.0mm, and the thickness of a shell layer is 0.1-0.15mm) is formed, as shown in figures 2 and 3. By scraping different numbers of the smell balls, longan fragrance with different concentrations can be smelled.
Example 2
This example illustrates a patterned microarray of odor molecules and preparation thereof
5g of milk essence solution (water-based spice), 2g of ethylene glycol and 2g of polyvinyl alcohol (PVA, 10 wt%) are uniformly mixed to form suspension containing odor molecules, the suspension is loaded into an ink box of an ink-jet printer after being prepared, a printing nozzle with the inner diameter of 0.06mm is selected, and a 9X 4 dot matrix liquid drop pattern is printed on a PET film substrate according to a preset pattern. Poly (lactic-co-glycolic acid) (PLGA, 2 wt%, solvent is dichloromethane) is loaded into an ink box of an ink-jet printer, a printing nozzle with the inner diameter of 0.06mm is selected, in-situ printing is carried out according to a preset pattern, the printing nozzle is covered on perfume molecular ink drops, PLGA solid is separated out after the dichloromethane is volatilized, and a core-shell structure is formed (the core-shell structure is hemispherical with the grain diameter of 0.9-1.15 mm; in the core-shell structure, the grain diameter of a core layer is 0.8-1.0mm, and the thickness of a shell layer is 0.1-0.15mm), as shown in figure 3. By scraping different numbers of the smell balls, milk fragrance of different concentrations can be smelled.
Example 3
This example illustrates a patterned microarray of odor molecules and preparation thereof
5g of vanilla essence solution (oily flavor), 3g of ethylene glycol and 3g of polyvinyl alcohol (PVA, 10 wt%) are uniformly mixed to form suspension containing odor molecules, the suspension is loaded into an ink box of an ink-jet printer after being prepared, a printing nozzle with the inner diameter of 0.06mm is selected, and a 9X 4 dot matrix liquid drop pattern is printed on a PET film substrate according to a preset pattern. Poly (lactic-co-glycolic acid) (PLGA, 3 wt%, solvent is dichloromethane) is loaded into an ink box of an ink-jet printer, a printing nozzle with the inner diameter of 0.06mm is selected, in-situ printing is carried out according to a preset pattern, the printing nozzle is covered on perfume molecular ink drops, PLGA solid is separated out after the dichloromethane is volatilized, and a core-shell structure is formed (the core-shell structure is hemispherical with the grain diameter of 0.9-1.15 mm; in the core-shell structure, the grain diameter of a core layer is 0.8-1.0mm, and the thickness of a shell layer is 0.1-0.15mm), as shown in figure 3. By scraping different numbers of scent balls, different concentrations of vanilla scent can be smelled.
Example 4
This example illustrates a patterned microarray of odor molecules and preparation thereof
Mixing 5g longan essence solution (water-based perfume), 2.5g ethylene glycol and 4.5g polyvinyl alcohol (PVA, 10 wt%) to obtain suspension containing odor molecules, loading into ink box of ink-jet printer, selecting printing nozzle with inner diameter of 0.06mm, and printing on PET film substrate according to preset patternPrinting-out
And
dot matrix drop pattern of (a). Poly (lactic-co-glycolic acid) (PLGA, 2.5 wt%, solvent is dichloromethane) is loaded into an ink box of an ink-jet printer, a printing nozzle with the inner diameter of 0.06mm is selected, in-situ printing is carried out according to a preset pattern, the printing nozzle is covered on perfume molecular ink drops, and PLGA solid is separated out after the dichloromethane is volatilized, so that a core-shell structure (the core-shell structure is hemispherical with the particle size of 0.9-1.15mm, in the core-shell structure, the particle size of a core layer is 0.8-1.0mm, and the thickness of a shell layer is 0.1-0.15mm) is formed, as shown in figure 4. By scraping different numbers of the smell balls, longan fragrance with different concentrations can be smelled.
Example 5
This example illustrates a patterned microarray of odor molecules and preparation thereof
Uniformly mixing 5g of longan essence solution (water-based spice), 2.5g of ethylene glycol and 4.5g of polyvinyl alcohol (PVA, 10 wt%) to obtain a suspension containing odor molecules, filling the suspension into an ink box of an ink-jet printer after preparation, selecting a printing nozzle with the inner diameter of 0.06mm, and printing a 'mil' dot matrix droplet pattern on a PET film substrate according to a preset pattern. Poly (lactic-co-glycolic acid) (PLGA, 2.5 wt%, solvent is dichloromethane) is loaded into an ink box of an ink-jet printer, a printing nozzle with the inner diameter of 0.06mm is selected, in-situ printing is carried out according to a preset pattern, the printing nozzle is covered on perfume molecular ink drops, and PLGA solid is separated out after the dichloromethane is volatilized, so that a core-shell structure (the core-shell structure is hemispherical with the particle size of 0.9-1.15mm, in the core-shell structure, the particle size of a core layer is 0.8-1.0mm, and the thickness of a shell layer is 0.1-0.15mm) is formed, as shown in figure 4. By scraping different numbers of the smell balls, longan fragrance with different concentrations can be smelled.
Example 6
This example illustrates a patterned microarray of odor molecules and preparation thereof
Uniformly mixing 5g of longan essence solution (water-based spice), 2.5g of ethylene glycol and 4.5g of polyvinyl alcohol (PVA, 10 wt%) to obtain a suspension containing odor molecules, filling the suspension into an ink box of an ink-jet printer after preparation, selecting a printing nozzle with the inner diameter of 0.06mm, and respectively printing 9 x 4 dot matrix droplet patterns on a glass sheet, a silicon wafer (hard material) and a PET film (flexible material) according to preset patterns. Poly (lactic-co-glycolic acid) (PLGA, 2.5 wt%, solvent is dichloromethane) is loaded into an ink box of an ink-jet printer, a printing nozzle with the inner diameter of 0.06mm is selected, in-situ printing is carried out according to a preset pattern, the printing nozzle is covered on perfume molecular ink drops, when the dichloromethane volatilizes to separate out PLGA solid, the materials of the three substrates can form a core-shell structure (the core-shell structure is hemispherical with the grain diameter of 0.9-1.15 mm; in the core-shell structure, the grain diameter of the core layer is 0.8-1.0mm, and the thickness of the shell layer is 0.1-0.15mm), as shown in figure 5. By scraping different numbers of the smell balls, longan fragrance with different concentrations can be smelled.
Example 7
This example illustrates a patterned microarray of odor molecules and preparation thereof
5g of 25-28% ammonia water solution, 2.5g of ethylene glycol and 4.5g of polyvinyl alcohol (PVA, 10 wt%) are uniformly mixed to form a suspension containing odor molecules, the suspension is loaded into an ink box of an ink-jet printer after preparation, a printing nozzle with the inner diameter of 0.06mm is selected, and a 9X 4 dot matrix liquid drop pattern is printed on a PET film substrate according to a preset pattern. Poly (lactic-co-glycolic acid) (PLGA, 2.5 wt%, solvent is dichloromethane) is filled into an ink box of an ink-jet printer, a printing nozzle with the inner diameter of 0.06mm is selected, in-situ printing is carried out according to a preset pattern, the printing nozzle is covered on perfume molecular ink drops, and PLGA solid is separated out after the dichloromethane is volatilized to form a core-shell structure (the core-shell structure is hemispherical with the grain diameter of 0.9-1.15mm, in the core-shell structure, the grain diameter of a core layer is 0.8-1.0mm, and the thickness of a shell layer is 0.1-0.15 mm). Preparing a precision test paper with the pH value of 8.2-10.0, and wetting the precision test paper with secondary water (the pH value is 5.9) for standby; after scratching different numbers of odor balls, the odor emitted was detected using a wetted test strip, as shown in fig. 6, which was seen to turn gradually blue as the amount of odor balls scratched increased.
Example 8
This example illustrates a patterned microarray of odor molecules and preparation thereof
5g of rose essence solution (oily spice), 2.5g of ethylene glycol and 4.5g of polyvinyl alcohol (PVA, 10 wt%) are uniformly mixed to form suspension containing odor molecules, the suspension is loaded into an ink box of an ink-jet printer after preparation, a printing nozzle with the inner diameter of 0.06mm is selected, and a 9X 4 dot matrix liquid drop pattern is printed on a PET film according to a preset pattern. Poly (lactic-co-glycolic acid) (PLGA, 2.5 wt%, solvent is dichloromethane) is filled into an ink box of an ink-jet printer, a printing nozzle with the inner diameter of 0.06mm is selected, in-situ printing is carried out according to a preset pattern, the printing nozzle is covered on perfume molecular ink drops, PLGA solid is separated out after the dichloromethane is volatilized, and materials of three substrates can form a core-shell structure (the core-shell structure is a hemisphere with the particle size of 0.9-1.15mm, in the core-shell structure, the particle size of a core layer is 0.8-1.0mm, and the thickness of a shell layer is 0.1-0.15 mm). By scraping different numbers of scent balls, different concentrations of rose scent can be smelled.
Example 9
This example illustrates a patterned microarray of odor molecules and preparation thereof
Preparation of a patterned microarray of odor molecules was performed according to the method of example 1, except that poly (lactic-co-glycolic acid) was used at concentrations of 0.5 wt%, 1 wt%, 1.5 wt%, 2 wt%, 2.25 wt%, 2.5 wt%, 3 wt%, 5 wt%, respectively.
Example 10
This example illustrates a patterned microarray of odor molecules and preparation thereof
The preparation of a patterned microarray of odor molecules was performed according to the method of example 1, except that,
comparative example
This comparative example illustrates the production of a reference odor molecule and its preparation
Referring to the components and formulation of example 1, except that the perfume was directly sprayed on the a4 printing paper using the conventional perfume test paper preparation method, the fragrance was not noticeable within 2 days.
Test example 1
Preparation of KMnO4Solution (6.13X 10)-5mg/mL, several portions, 15mL each, in a petri dish ready for use), samples prepared in the above examples were taken for testing, each sample was scraped with a different number of odor spheres and placed in KMnO4In solution, the microarrays prepared in examples 1-8 changed from purple to orange-red, then orange, and finally yellow as the amount of scraped odor spheres increased. It was found that the odor was stable without leakage without scraping the odor ball, as shown in fig. 7.
In example 9, when the concentration of poly (lactic acid-co-glycolic acid) is less than 2 wt%, the core-shell coating structure is formed, but the substance in the core leaks to a different extent; when the concentration is higher than 3 wt%, a core-shell coating structure can be formed, but substances in the core are difficult to release at the later stage; at concentrations between 2 and 3 wt%, a change of the solution from purple to orange-red, then orange, and finally yellow, occurs as the amount of odor spheres scraped increases.
Test example 2
Preparation of KMnO4Solution (6.13X 10)-5mg/mL, several portions, 15mL each, in a petri dish ready for use), samples prepared in examples 1-9 were taken for testing, placed in KMnO4 solution, and left to stand for 24 hours. After 24 hours, the solutions of examples 1-8 did not change color (as shown in fig. 7), and no odor ball was observed, and the odor was stable and did not leak. In addition, the samples of examples 1-9 were stored in air and scraped off after one month, and examples 1-8 still had a strong aroma, whereas the taste was not noticeable after 2 days of trial manufacture of the comparative example, as described above.
In example 9, when the poly (lactic acid-co-glycolic acid) concentration is less than 2 wt%, although the core-shell coating structure can be formed, substances in the core leak to different degrees, and the color of the solution changes; when the concentration is between 2 and 5 wt%, the color of the solution is not changed. After one month of storage, the samples with a concentration of 2-3 wt% poly (lactic-co-glycolic acid) still had a strong fragrance and a weak odor.
The preferred embodiments of the present invention have been described above in detail, but the present invention is not limited thereto. Within the scope of the technical idea of the invention, many simple modifications can be made to the technical solution of the invention, including combinations of various technical features in any other suitable way, and these simple modifications and combinations should also be regarded as the disclosure of the invention, and all fall within the scope of the invention.
Claims (10)
1. A patterned microarray of odor molecules, comprising a substrate and a core-shell structure formed on the substrate, wherein the core-shell structure comprises odor molecules as a core and poly (lactic-co-glycolic acid) as a shell.
2. The microarray of claim 1, wherein the core-shell structure is hemispherical with a particle size of 0.5-1.5 mm;
preferably, in the core-shell structure, the particle size of the core layer is 0.8-1.0mm, and the thickness of the shell layer is 0.10-0.15 mm.
3. The patterned microarray of claim 1 or 2, wherein the scent molecules are selected from one or more of alcohols, aldehydes, esters, proteins, milk essence, longan essence, vanilla essence, cola essence, apple essence, cumin essence, lemon essence, walnut essence, grape essence, almond essence, rose essence, and ammonia; and/or
The substrate is selected from one of a silicon chip, a glass sheet, a quartz sheet, an iron sheet, a copper sheet, an aluminum oxide sheet, a PDMS film, a PET film, a PS film, a PU film and a PI film.
4. The patterned microarray of any of claims 1-3, wherein the patterned microarray is a lattice pattern of the core-shell structures;
preferably, the lattice pattern is a periodic array of single dots.
5. A method of making a patterned microarray of odorant molecules, the method comprising:
(1) printing a suspension containing scent molecules on a substrate in a predetermined pattern;
(2) printing a poly (lactic-co-glycolic acid) solution in situ on the suspension of the odor molecules printed on the substrate, and volatilizing the solvent in the poly (lactic-co-glycolic acid) solution to obtain the patterned microarray having a core-shell structure with the odor molecules as cores and the poly (lactic-co-glycolic acid) as shells arranged according to a predetermined pattern.
6. The method of claim 5, wherein the odor molecule-containing suspension consists of odor molecules, emulsifiers, alcohols, and solvents;
preferably, in the suspension containing the odor molecules, the content of the odor molecules is 0.01 to 50 wt.%, the content of the alcohol is 2 to 3 wt.%, and the content of the emulsifier is 0.25 to 5 wt.%;
preferably, the emulsifier is polyvinyl alcohol;
preferably, the solvent is water;
preferably, the alcohol is ethylene glycol.
7. The method of claim 5 or 6, wherein the printing in step (1) and step (2) is each independently inkjet printing.
8. The method of claim 5, wherein the poly (lactic-co-glycolic acid) solution is a dichloromethane solution of poly (lactic-co-glycolic acid);
preferably, the concentration of poly (lactic-co-glycolic acid) in the poly (lactic-co-glycolic acid) solution is 2 to 3% by weight.
9. A patterned microarray produced by the method of any one of claims 5-8.
10. Use of the patterned microarray of any of claims 1-4 and 9 in the preparation of products for scent on demand release, olfactory sensitivity detection, and olfactory sensitivity exercise.
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