CN110832322A - 利用双光子荧光测定法确定蛋白质结构的方法 - Google Patents

利用双光子荧光测定法确定蛋白质结构的方法 Download PDF

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CN110832322A
CN110832322A CN201880044826.4A CN201880044826A CN110832322A CN 110832322 A CN110832322 A CN 110832322A CN 201880044826 A CN201880044826 A CN 201880044826A CN 110832322 A CN110832322 A CN 110832322A
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protein
light
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巴森·克兰西
约书亚·萨拉夫斯基
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BlueLight Therapeutics Inc
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    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/636—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited using an arrangement of pump beam and probe beam; using the measurement of optical non-linear properties
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    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
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    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6445—Measuring fluorescence polarisation
    • G—PHYSICS
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    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
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    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
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    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
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    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
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    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)

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  • Optics & Photonics (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Nonlinear Science (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Optical Measuring Cells (AREA)
CN201880044826.4A 2017-05-03 2018-04-24 利用双光子荧光测定法确定蛋白质结构的方法 Pending CN110832322A (zh)

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US201762500912P 2017-05-03 2017-05-03
US62/500,912 2017-05-03
PCT/US2018/029234 WO2018204135A2 (en) 2017-05-03 2018-04-24 Methods of determining protein structure using two-photon fluorescence measurements

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US (1) US20200132604A1 (enExample)
EP (1) EP3619537A4 (enExample)
JP (1) JP2020519866A (enExample)
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WO (1) WO2018204135A2 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112102900A (zh) * 2020-10-12 2020-12-18 北京晶派科技有限公司 一种基于TopoMA定量构效关系模型的药物设计方法
CN114113314A (zh) * 2021-11-25 2022-03-01 厦门大学 一种用于滨海核电站的海洋致灾生物水声监测系统及方法
CN115356276A (zh) * 2022-07-26 2022-11-18 武汉新烽光电股份有限公司 含有固体残留物的检测试剂的吸光度值估计方法及系统

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016106286A1 (en) 2014-12-23 2016-06-30 Biodesy, Inc. Attachment of proteins to interfaces for use in nonlinear optical detection
WO2017196891A1 (en) 2016-05-09 2017-11-16 Biodesy, Inc. Methods and devices for detection of peripheral membrane protein interactions using nonlinear optical techniques
EP4191236A1 (en) * 2017-05-23 2023-06-07 Hamamatsu Photonics K.K. Orientation characteristic measurement method, orientation characteristic measurement program, and orientation characteristic measurement device
US11500352B2 (en) * 2019-05-01 2022-11-15 Dh Technologies Development Pte. Ltd. System and method for monitoring a production process
CN113456089B (zh) * 2021-06-30 2023-12-05 中国科学院半导体研究所 一种兼顾电生理信号记录的微型荧光成像系统
WO2023095154A1 (en) * 2021-11-24 2023-06-01 Sensoville Biotech Pvt Ltd Method for determining changes in a protein's structure
CN118737306B (zh) * 2024-06-28 2025-09-19 西安电子科技大学 基于强化隐扩散模型的共价药物从头设计方法及相关装置

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104583776A (zh) * 2012-04-25 2015-04-29 比奥德赛公司 用于检测蛋白质的变构调节剂的方法
US20150119270A1 (en) * 2012-02-05 2015-04-30 Biodesy,Inc. Methods for detecting allosteric modulators of protein
US20160292354A1 (en) * 2015-04-02 2016-10-06 Biodesy, Inc. Methods for determining protein structure using a surface-selective nonlinear optical technique

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU5054198A (en) * 1996-12-03 1998-06-29 Erkki Soini Biospecific, two photon excitation, fluorescence detection and device
US6953694B2 (en) * 2000-12-06 2005-10-11 The Trustees Of Columbia University In The City Of New York Attachment of second harmonic-active moiety to molecules for detection of molecules at interfaces
US7253287B1 (en) * 2004-05-14 2007-08-07 Research Foundation Of The University Of Central Florida, Inc. Reactive probes for two-photon fluorescent imaging and sensing
WO2016106286A1 (en) * 2014-12-23 2016-06-30 Biodesy, Inc. Attachment of proteins to interfaces for use in nonlinear optical detection

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150119270A1 (en) * 2012-02-05 2015-04-30 Biodesy,Inc. Methods for detecting allosteric modulators of protein
CN104583776A (zh) * 2012-04-25 2015-04-29 比奥德赛公司 用于检测蛋白质的变构调节剂的方法
US20160292354A1 (en) * 2015-04-02 2016-10-06 Biodesy, Inc. Methods for determining protein structure using a surface-selective nonlinear optical technique

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112102900A (zh) * 2020-10-12 2020-12-18 北京晶派科技有限公司 一种基于TopoMA定量构效关系模型的药物设计方法
CN112102900B (zh) * 2020-10-12 2024-02-23 北京晶泰科技有限公司 一种基于TopoMA定量构效关系模型的药物设计方法
CN114113314A (zh) * 2021-11-25 2022-03-01 厦门大学 一种用于滨海核电站的海洋致灾生物水声监测系统及方法
CN114113314B (zh) * 2021-11-25 2024-03-22 厦门大学 一种用于滨海核电站的海洋致灾生物水声监测系统及方法
CN115356276A (zh) * 2022-07-26 2022-11-18 武汉新烽光电股份有限公司 含有固体残留物的检测试剂的吸光度值估计方法及系统

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US20200132604A1 (en) 2020-04-30
EP3619537A4 (en) 2021-03-10
WO2018204135A3 (en) 2018-12-13
JP2020519866A (ja) 2020-07-02
EP3619537A2 (en) 2020-03-11

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Application publication date: 20200221