CN110830768B - Method and device for acquiring and processing colony image - Google Patents
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Abstract
The embodiment of the invention discloses a method and a device for acquiring and processing a colony image, wherein the method comprises the following steps: acquiring current environment data, and determining an image recording standard according to the environment data; and acquiring a current colony image according to a preset period, and acquiring the colony image if the colony image is judged to meet the image recording standard. According to the embodiment of the invention, the image recording standard is determined through the environmental data, and different image recording standards can be determined according to different growth speeds of bacterial colonies in different environments, so that bacterial colony images meeting the image recording standard are collected for analysis and processing, the bacterial colony images do not need to be collected frequently, a large number of bacterial colony images do not need to be analyzed and processed, the processing performance of the system is greatly improved, and the storage space of the system is greatly saved.
Description
Technical Field
The invention relates to the technical field of computers, in particular to a method and a device for acquiring and processing a colony image.
Background
In the microbiological examination of food, medicine, cosmetics, feed and the like, the items such as total bacterial colony number, coliform bacteria, escherichia coli, lactic acid bacteria and the like are involved, and a large amount of bacterial colony analysis and counting are needed in the statistical result. The colony is a single bacterium group which grows on the surface of a solid culture medium and can be seen by naked eyes after the bacterium is inoculated on the surface of the solid culture medium and cultured. Counting the number of colonies generated after sampling a target is a fundamental and important task in the related art. The colony image is an image formed by shooting the colony with an industrial camera after the colony is cultured on a culture medium, so that the colony target information is counted conveniently.
At present, the work of colony analysis is mainly completed by the observation of artificial naked eyes, and the work is heavy, tedious and low in efficiency, poor in detection repeatability, low in precision, incapable of storing original data and the like. The automatic colony analysis device can overcome the defects of manual analysis and counting, and the automation equipment for colony analysis in the prior art greatly reduces the processing performance of the system due to the fact that images are collected too frequently, and simultaneously greatly wastes the storage space of the system.
Disclosure of Invention
Because the existing method has the problems, the embodiment of the invention provides a method and a device for acquiring and processing a colony image.
In a first aspect, an embodiment of the present invention provides a method for acquiring and processing a colony image, including:
acquiring current environment data, and determining an image recording standard according to the environment data;
and acquiring a current colony image according to a preset period, and acquiring the colony image if the colony image is judged to meet the image recording standard.
Optionally, the acquiring current environment data and determining an image recording standard according to the environment data specifically include:
acquiring current temperature data and humidity data, and determining a colony diameter threshold corresponding to the current temperature data and humidity data according to an environment data growth diameter correspondence table;
correspondingly, the current colony image is obtained according to the preset period, and if the colony image is judged to meet the image recording standard, the colony image is collected, and the method specifically comprises the following steps:
acquiring a current colony image according to a preset period, and acquiring the colony image if the diameter of a new first colony appearing in the colony image is larger than the colony diameter threshold value.
Optionally, the acquiring current environment data and determining an image recording standard according to the environment data specifically include:
acquiring current temperature data and humidity data, and determining a bacterial colony growth threshold corresponding to the current temperature data and humidity data according to an environment data growth speed correspondence table;
correspondingly, the current colony image is obtained according to the preset period, and if the colony image is judged to meet the image recording standard, the colony image is collected, and the method specifically comprises the following steps:
and acquiring a current colony image according to a preset period, and acquiring the colony image if the difference value between the diameter of the second colony in the colony image and the diameter of the second colony in the last recorded colony image is larger than the colony growth threshold value.
Optionally, the obtaining a current colony image according to a preset period, and if it is determined that the diameter of a new first colony appearing in the colony image is greater than the colony diameter threshold, after the collecting the colony image, further includes:
acquiring the number of newly added bacterial colonies of newly appeared bacterial colonies with the diameter larger than the diameter threshold of the bacterial colonies in the bacterial colony image;
and obtaining the current colony number according to the existing colony number and the newly added colony number.
Optionally, the obtaining a current colony image according to a preset period, and if it is determined that the colony image meets the image recording standard, acquiring the colony image, specifically including:
and receiving the current colony image sent by a terminal according to a preset period, and acquiring the colony image if the colony image is judged to meet the image recording standard.
In a second aspect, an embodiment of the present invention further provides an apparatus for acquiring and processing a colony image, including:
the standard determining module is used for acquiring current environment data and determining an image recording standard according to the environment data;
and the image acquisition module is used for acquiring the current colony image according to a preset period, and acquiring the colony image if the colony image is judged to accord with the image recording standard.
Optionally, the standard determination module is specifically configured to:
acquiring current temperature data and humidity data, and determining a colony diameter threshold corresponding to the current temperature data and humidity data according to an environment data growth diameter correspondence table;
correspondingly, the image acquisition module is specifically configured to:
acquiring a current colony image according to a preset period, and acquiring the colony image if the diameter of a new first colony appearing in the colony image is larger than the colony diameter threshold value.
Optionally, the standard determination module is specifically configured to:
acquiring current temperature data and humidity data, and determining a bacterial colony growth threshold corresponding to the current temperature data and humidity data according to an environment data growth speed correspondence table;
correspondingly, the image acquisition module is specifically configured to:
and acquiring a current colony image according to a preset period, and acquiring the colony image if the difference value between the diameter of the second colony in the colony image and the diameter of the second colony in the last recorded colony image is larger than the colony growth threshold value.
Optionally, the device for acquiring and processing colony images further comprises:
the number obtaining module is used for obtaining the number of newly added bacterial colonies of newly appeared bacterial colonies with the diameter larger than the diameter threshold value of the bacterial colonies in the bacterial colony image;
and the quantity updating module is used for obtaining the current colony quantity according to the existing colony quantity and the newly added colony quantity.
Optionally, the image acquisition module is specifically configured to:
and receiving the current colony image sent by a terminal according to a preset period, and acquiring the colony image if the colony image is judged to meet the image recording standard.
In a third aspect, an embodiment of the present invention further provides an electronic device, including:
at least one processor; and
at least one memory communicatively coupled to the processor, wherein:
the memory stores program instructions executable by the processor, which when called by the processor are capable of performing the above-described methods.
In a fourth aspect, an embodiment of the present invention further provides a non-transitory computer-readable storage medium storing a computer program, which causes the computer to execute the above method.
According to the technical scheme, the image recording standard is determined through the environment data, and different image recording standards can be determined according to different growth speeds of bacterial colonies in different environments, so that bacterial colony images meeting the image recording standard are collected for analysis and processing, the bacterial colony images do not need to be collected frequently, a large number of bacterial colony images do not need to be analyzed and processed, the processing performance of the system is greatly improved, and the storage space of the system is greatly saved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
Fig. 1 is a schematic flow chart of a method for acquiring and processing a colony image according to an embodiment of the present invention;
fig. 2 is a schematic structural diagram of a device for acquiring and processing a colony image according to an embodiment of the present invention;
fig. 3 is a logic block diagram of an electronic device according to an embodiment of the present invention.
Detailed Description
The following further describes embodiments of the present invention with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and the protection scope of the present invention is not limited thereby.
Fig. 1 shows a schematic flow chart of a method for acquiring and processing a colony image provided in this embodiment, including:
s101, obtaining current environment data, and determining an image recording standard according to the environment data.
Wherein the environmental data is data related to the environment, such as temperature data and humidity data.
The image recording standard is a standard for judging whether an image needs to be acquired, for example, the diameter of a new colony appearing in the colony image satisfies a condition, or the growth speed of an existing colony in the colony image and the growth speed of a colony recorded last time satisfy a condition.
Specifically, in the process of collecting the colony image, the colony continuously grows, the growth speed of the colony is closely related to the external environment, particularly has a large relation with the temperature and the humidity, so that the image recording standard can be determined according to the current temperature data and the current humidity data, and whether the current colony image is collected or not can be determined according to the standard.
S102, acquiring a current colony image according to a preset period, and acquiring the colony image if the colony image is judged to meet the image recording standard.
Wherein the preset period is predetermined according to the general growth rate of the colonies, for example, the current colony image is taken every 5 minutes.
Specifically, after a current colony image is obtained, judging a colony in the colony image, and collecting the current colony image when the colony in the image meets an image recording standard; when the colonies in the image do not meet the image registration criteria, then no current colony image is acquired.
The image recording standard is confirmed through environmental data to this embodiment, can confirm different image recording standards according to the different growth rates of bacterial colony in the different environment to the bacterial colony image that the collection accords with image recording standard is used for analysis and processing, need not frequently to gather the bacterial colony image, also need not to carry out analysis processes to a large amount of bacterial colony images, has improved the processing performance of system greatly, has saved the storage space of system greatly simultaneously.
Further, on the basis of the above method embodiment, S101 specifically includes:
and acquiring current temperature data and humidity data, and determining a colony diameter threshold corresponding to the current temperature data and humidity data according to the environment data growth diameter correspondence table.
Correspondingly, S102 specifically includes:
acquiring a current colony image according to a preset period, and acquiring the colony image if the diameter of a new first colony appearing in the colony image is larger than the colony diameter threshold value.
Wherein the temperature data is the current temperature in the colony culture dish.
The humidity data is the humidity in the current colony culture dish.
The environment data growth diameter corresponding table is a corresponding relation table of preset environment data and the growth diameter of the bacterial colony, and the growth diameter is a bacterial colony diameter threshold value used for subsequently judging the diameter of the bacterial colony.
The first colony is a colony with a diameter larger than a colony diameter threshold value in a currently acquired colony image.
The colony diameter threshold is a threshold for judging the colony diameter.
Specifically, the colony diameter threshold value corresponding to each different temperature and humidity is stored in the environment data growth diameter correspondence table, after the current temperature data and humidity data are obtained, the colony diameter threshold value corresponding to the temperature data and the humidity data can be directly determined according to the environment data growth diameter correspondence table, and whether the diameter of the first colony newly appearing in the currently obtained colony image is larger than the colony diameter threshold value or not is judged through the colony diameter threshold value. And when the diameter of the first colony is larger than the colony diameter threshold value, acquiring a current colony image, otherwise, not acquiring.
For example, the current temperature in the colony culture dish is 25 degrees, the humidity is 40%, the growth diameter corresponding table can be obtained according to the query environment data, and the colony diameter threshold value corresponding to 25 degrees and 40% is 30 micrometers. There are 3 newly grown colonies in the colony image who obtains, be colony 1, colony 2 and colony 3 respectively, wherein colony 1's diameter is 20 microns, and colony 2's diameter is 35 microns, and colony 3's diameter is 33 microns, because colony 2 and colony 3's diameter all is greater than 30 microns, consequently need gather current colony image.
Whether the colony image is collected is judged through the diameter of the colony, and the colony image with analysis value can be collected by combining the growth condition of a new colony.
In another embodiment, S101 specifically includes:
and acquiring current temperature data and humidity data, and determining a bacterial colony growth threshold corresponding to the current temperature data and humidity data according to the environment data growth speed corresponding table.
Correspondingly, S102 specifically includes:
and acquiring a current colony image according to a preset period, and acquiring the colony image if the difference value between the diameter of the second colony in the colony image and the diameter of the second colony in the last recorded colony image is larger than the colony growth threshold value.
Wherein the colony growth threshold is a threshold for judging the colony growth speed.
The environment data growth speed corresponding table is a corresponding relation table of preset environment data and the growth speed of the bacterial colony, and the growth speed is a threshold value for subsequently judging the growth size of the diameter of the bacterial colony.
Specifically, the colony growth threshold corresponding to each different temperature and humidity is stored in the environment data growth speed corresponding table, after the current temperature data and humidity data are obtained, the colony growth threshold corresponding to the temperature data and the humidity data can be directly determined according to the environment data growth diameter corresponding table, the difference value between the diameter of the existing second colony in the currently obtained colony image and the diameter of the second colony in the last recorded colony image is judged to be larger than the colony growth threshold through the colony growth threshold, then the current colony image is collected, and otherwise, the current colony image is not collected.
For example, the current temperature in the colony culture dish is 25 degrees, the humidity is 40%, the current temperature can be obtained by inquiring the environment data growth diameter corresponding table, and the colony growth threshold value corresponding to 25 degrees and 40% is 10 micrometers. All 6 colonies in the colony images obtained, 2 of which were newly grown and 4 of which were present. The 4 existing colonies are colony 4, colony 5 and colony 6 respectively, wherein the diameter of colony 4 increases to 20 microns, the diameter of colony 5 increases to 8 microns, the diameter of colony 6 increases to 12 microns, because the diameter of colony 4 and colony 6 increases and is all greater than 10 microns, therefore need gather current colony image.
By judging the growth speed of the bacterial colony in the bacterial colony images of the previous and next two times, the bacterial colony image with analysis value can be collected by combining the growth condition of the existing bacterial colony.
Further, on the basis of the above method embodiment, after S102, the method further includes:
s103, acquiring the number of newly added colonies of the newly appeared colonies with the diameter larger than the colony diameter threshold value in the colony image.
And S104, obtaining the current colony number according to the existing colony number and the newly added colony number.
Wherein, the newly increased colony quantity is newly increased colony quantity in the colony image of gathering.
The number of the existing colonies is the number of the colonies in the colony image collected last time.
And adding the number of the existing colonies and the number of the newly added colonies to obtain the total number of the colonies in the current colony image.
Specifically, count through newly-increased colony in to current colony image, combine the colony quantity of record in the last collection image simultaneously, obtain current colony quantity, only need record newly-increased colony quantity in the newly-gathered image can, adopt the mode of accumulating up to obtain total colony quantity in the current colony image, can realize the accurate count of colony.
Further, on the basis of the above method embodiment, the S102 specifically includes:
and receiving the current colony image sent by a terminal according to a preset period, and acquiring the colony image if the colony image is judged to meet the image recording standard.
Specifically, after the terminal acquires the bacterial colony image, send this bacterial colony image to the high in the clouds, carry out the analysis to this bacterial colony image by the high in the clouds, after the bacterial colony image accords with the image record standard, gather this bacterial colony image, realize the automatic acquisition of cloud to the bacterial colony image to and the automatic counting of bacterial colony.
Fig. 2 is a schematic structural diagram of an apparatus for acquiring and processing a colony image according to this embodiment, where the apparatus includes: a criterion determination module 201 and an image acquisition module 202, wherein:
the standard determining module 201 is configured to obtain current environment data and determine an image recording standard according to the environment data;
the image acquisition module 202 is configured to acquire a current colony image according to a preset period, and acquire the colony image if it is determined that the colony image meets the image recording standard.
Specifically, the standard determining module 201 obtains current environment data, and determines an image recording standard according to the environment data; the image acquisition module 202 acquires a current colony image according to a preset period, and acquires the colony image if the colony image is judged to meet the image recording standard.
The image recording standard is confirmed through environmental data to this embodiment, can confirm different image recording standards according to the different growth rates of bacterial colony in the different environment to the bacterial colony image that the collection accords with image recording standard is used for analysis and processing, need not frequently to gather the bacterial colony image, also need not to carry out analysis processes to a large amount of bacterial colony images, has improved the processing performance of system greatly, has saved the storage space of system greatly simultaneously.
Further, on the basis of the above device embodiment, the criterion determining module 201 is specifically configured to:
acquiring current temperature data and humidity data, and determining a colony diameter threshold corresponding to the current temperature data and humidity data according to an environment data growth diameter correspondence table;
accordingly, the image acquisition module 202 is specifically configured to:
acquiring a current colony image according to a preset period, and acquiring the colony image if the diameter of a new first colony appearing in the colony image is larger than the colony diameter threshold value.
Further, on the basis of the above device embodiment, the criterion determining module 201 is specifically configured to:
acquiring current temperature data and humidity data, and determining a bacterial colony growth threshold corresponding to the current temperature data and humidity data according to an environment data growth speed correspondence table;
accordingly, the image acquisition module 202 is specifically configured to:
and acquiring a current colony image according to a preset period, and acquiring the colony image if the difference value between the diameter of the second colony in the colony image and the diameter of the second colony in the last recorded colony image is larger than the colony growth threshold value.
Further, on the basis of the above device embodiment, the device for acquiring and processing colony images further includes:
the number obtaining module is used for obtaining the number of newly added bacterial colonies of newly appeared bacterial colonies with the diameter larger than the diameter threshold value of the bacterial colonies in the bacterial colony image;
and the quantity updating module is used for obtaining the current colony quantity according to the existing colony quantity and the newly added colony quantity.
Further, on the basis of the above device embodiment, the image acquisition module 202 is specifically configured to:
and receiving the current colony image sent by a terminal according to a preset period, and acquiring the colony image if the colony image is judged to meet the image recording standard.
The device for collecting and processing colony images described in this embodiment can be used for executing the above method embodiments, and the principle and technical effect are similar, and are not described herein again.
Referring to fig. 3, the electronic device includes: a processor (processor)301, a memory (memory)302, and a bus 303;
wherein,
the processor 301 and the memory 302 complete communication with each other through the bus 303;
the processor 301 is configured to call program instructions in the memory 302 to perform the methods provided by the above-described method embodiments.
The present embodiments disclose a computer program product comprising a computer program stored on a non-transitory computer readable storage medium, the computer program comprising program instructions which, when executed by a computer, enable the computer to perform the methods provided by the above-described method embodiments.
The present embodiments provide a non-transitory computer-readable storage medium storing computer instructions that cause the computer to perform the methods provided by the method embodiments described above.
The above-described embodiments of the apparatus are merely illustrative, and the units described as separate parts may or may not be physically separate, and parts displayed as units may or may not be physical units, may be located in one place, or may be distributed on a plurality of network units. Some or all of the modules may be selected according to actual needs to achieve the purpose of the solution of the present embodiment. One of ordinary skill in the art can understand and implement it without inventive effort.
Through the above description of the embodiments, those skilled in the art will clearly understand that each embodiment can be implemented by software plus a necessary general hardware platform, and certainly can also be implemented by hardware. With this understanding in mind, the above-described technical solutions may be embodied in the form of a software product, which can be stored in a computer-readable storage medium such as ROM/RAM, magnetic disk, optical disk, etc., and includes instructions for causing a computer device (which may be a personal computer, a server, or a network device, etc.) to execute the methods described in the embodiments or some parts of the embodiments.
It should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (8)
1. A method for collecting and processing a colony image is characterized by comprising the following steps:
acquiring current environment data, and determining an image recording standard according to the environment data;
acquiring a current colony image according to a preset period, and acquiring the colony image if the colony image is judged to meet the image recording standard;
the acquiring of the current environment data and the determining of the image recording standard according to the environment data specifically include:
acquiring current temperature data and humidity data, and determining a colony diameter threshold corresponding to the current temperature data and humidity data according to an environment data growth diameter correspondence table;
correspondingly, the current colony image is obtained according to the preset period, and if the colony image is judged to meet the image recording standard, the colony image is collected, and the method specifically comprises the following steps:
acquiring a current colony image according to a preset period, and acquiring the colony image if the diameter of a new first colony appearing in the colony image is larger than the colony diameter threshold value;
obtain current colony image according to preset cycle, if judge that the diameter that appears new first colony in the colony image is greater than colony diameter threshold, then gather behind the colony image, still include:
acquiring the number of newly added bacterial colonies of newly appeared bacterial colonies with the diameter larger than the diameter threshold of the bacterial colonies in the bacterial colony image;
and obtaining the current colony number according to the existing colony number and the newly added colony number.
2. The method for acquiring and processing colony images as claimed in claim 1, wherein the acquiring current environment data and determining image recording standards according to the environment data specifically comprise:
acquiring current temperature data and humidity data, and determining a bacterial colony growth threshold corresponding to the current temperature data and humidity data according to an environment data growth speed correspondence table;
correspondingly, the current colony image is obtained according to the preset period, and if the colony image is judged to meet the image recording standard, the colony image is collected, and the method specifically comprises the following steps:
and acquiring a current colony image according to a preset period, and acquiring the colony image if the difference value between the diameter of the second colony in the colony image and the diameter of the second colony in the last recorded colony image is larger than the colony growth threshold value.
3. The method for acquiring and processing the colony image according to claim 1, wherein the current colony image is acquired according to a preset period, and if the colony image is judged to meet the image recording standard, the colony image is acquired, which specifically comprises:
and receiving the current colony image sent by a terminal according to a preset period, and acquiring the colony image if the colony image is judged to meet the image recording standard.
4. An apparatus for collecting and processing a colony image, comprising:
the standard determining module is used for acquiring current environment data and determining an image recording standard according to the environment data;
the image acquisition module is used for acquiring a current colony image according to a preset period, and acquiring the colony image if the colony image is judged to meet the image recording standard;
the standard determination module is specifically configured to:
acquiring current temperature data and humidity data, and determining a colony diameter threshold corresponding to the current temperature data and humidity data according to an environment data growth diameter correspondence table;
correspondingly, the image acquisition module is specifically configured to:
acquiring a current colony image according to a preset period, and acquiring the colony image if the diameter of a new first colony appearing in the colony image is larger than the colony diameter threshold value;
the colony image acquisition and processing device further comprises:
the number obtaining module is used for obtaining the number of newly added bacterial colonies of newly appeared bacterial colonies with the diameter larger than the diameter threshold value of the bacterial colonies in the bacterial colony image;
and the quantity updating module is used for obtaining the current colony quantity according to the existing colony quantity and the newly added colony quantity.
5. The device for collecting and processing colony images as claimed in claim 4, wherein the standard determination module is specifically configured to:
acquiring current temperature data and humidity data, and determining a bacterial colony growth threshold corresponding to the current temperature data and humidity data according to an environment data growth speed correspondence table;
correspondingly, the image acquisition module is specifically configured to:
and acquiring a current colony image according to a preset period, and acquiring the colony image if the difference value between the diameter of the second colony in the colony image and the diameter of the second colony in the last recorded colony image is larger than the colony growth threshold value.
6. The device for collecting and processing colony images as claimed in claim 4, wherein the image collecting module is specifically configured to:
and receiving the current colony image sent by a terminal according to a preset period, and acquiring the colony image if the colony image is judged to meet the image recording standard.
7. An electronic device comprising a memory, a processor and a computer program stored on the memory and executable on the processor, wherein the processor implements the method for collecting and processing colony images as claimed in any one of claims 1 to 3 when executing the program.
8. A non-transitory computer-readable storage medium, on which a computer program is stored, wherein the computer program, when executed by a processor, implements the method for acquiring and processing the colony image according to any one of claims 1 to 3.
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| WO2013192377A1 (en) * | 2012-06-20 | 2013-12-27 | Global Biodiagnostics | Portable diagnostic system and uses thereof |
| CN107580715A (en) * | 2015-04-23 | 2018-01-12 | Bd科斯特公司 | Method and system for automatic counting microbial colonies |
| CN107771212A (en) * | 2015-04-23 | 2018-03-06 | Bd科斯特公司 | Bacterium colony contrast is collected |
| CN108192814A (en) * | 2017-01-23 | 2018-06-22 | 英特科学公司 | The method and apparatus counted for bacterium colony |
| CN109722381A (en) * | 2019-03-05 | 2019-05-07 | 南华大学 | Device and method for microculture and growth monitoring |
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