CN1108305A

CN1108305A - Process for prepn. of amino-acid by fermentation

Info

Publication number: CN1108305A
Application number: CN 94102211
Authority: CN
Inventors: 蒋庆林; 孙安帮; 孙凯
Original assignee: Individual
Current assignee: Individual
Priority date: 1994-03-11
Filing date: 1994-03-11
Publication date: 1995-09-13

Abstract

Distillers' grains is used as main raw material which is added with vegetable oil, ammonium bicarbonate and potassium dihydrogen phosphate and mixed thoroughly, then adding hydrochloric acid, mixing and regulating the pH value to 1-2, bagging immediately to ferment, controlling the temp. in bag at 30-40 deg.C, setting 12 days, adding NaOH, mixing and adjusting the pH value to 6, setting 12 hr, and separating the solid and liquid to obtain the amino acid bearing extract. Said technology features high finished product content, amino acid being 19%, humic acid being 4%, lower cost, simple process and good economic and social beneficial results.

Description

Process for prepn. of amino-acid by fermentation

The present invention relates to a kind of technology for extracting amino acid.

Amino acid generally adopts the biological chemistry multicomponent reaction to produce, it is that raw material extracts method of compound amino-acid with high protein things such as earthworm, silk cocoons that name is called " extract method of compound amino-acid and disacidify equipment " from the high protein thing patent application document (number of patent application 85109664) discloses a kind of, its method is: raw material processing, acid catalyzed hydrolysis, disacidify, detoxification, battle arrayization purification, sterilization etc., reparation technology is complicated, and it is higher to produce cost.

The object of the present invention is to provide that a kind of to produce cost lower, the better simply amino acid new process of production of reparation technology.

The object of the present invention is achieved like this:

With vinasse is main raw material, adds vegetables oil, bicarbonate of ammonia, potassium primary phosphate, and the back that stirs adds hydrochloric acid (HCl), stir and transfer pH value PH1-2, instant pack is fermented, temperature is controlled at 30-40 ℃ in the bag, place after 8-12 days, add NaOH, stir, transfer pH value to PH6, place after 12 hours, carry out solid-liquid separation, must contain amino acid, humic acid extracting solution.

Each component content (weight percent): vinasse 94.6-96.5%, vegetables oil 0.65-1.5%, bicarbonate of ammonia 2.1-3.2%, potassium primary phosphate 0.6-0.8%.

The extracting solution that adopts technology of the present invention to produce contains amino acid/11 9%, humic acid 4%, finished product content height, the amino acid cost is 440 yuan/ton, the humic acid cost is 520 yuan/ton, cost is lower, and production technique is simple, raw material sources are extensive, and the tankage vinasse of brewery are fully used, turn waste into wealth, have favorable economic benefit and social benefit.

Below in conjunction with embodiment the present invention is described in detail.

Following table is depicted as specific embodiment of the invention component content: (weight percent)

Component	Content (%)
				Vinasse	94.6	95.3	96.5
Vegetables oil	1.4	1.1	0.8
				Bicarbonate of ammonia	3.2	2.9	2.1
Potassium primary phosphate	0.8	0.7	0.6
				Content	100	100	100

As above arbitrary group of component content shown in the table can be by following explained hereafter, adopt first group of component content: wherein vegetables oil can be soya-bean oil or peanut oil, 189.2 kilograms in vinasse (94.6%), 2.8 kilograms of vegetables oil (1.4%), 6.4 kilograms in bicarbonate of ammonia (3.2%), 1.6 kilograms of potassium primary phosphates (0.8%).Back adding hydrochloric acid (HCl) stirs, stir, transfer pH value to PH1-2, can be PH1 or PH2, instant pack, every bag of 25kg, ferment, the bag in temperature be controlled at 30-40 ℃, the bag in a temperature be controlled at 30 ℃, 35 ℃, 40 ℃ all can, place after 8-12 days, place and all could in 8 days, 10 days, 12 days, add NaOH, stir, transfer pH value to PH6, place after 12 hours, put into plate-and-frame filter press (450 type) and carry out solid-liquid separation, aminoacids content is 19% in the isolated liquid, and the content of humic acid is 4%.Residue contains more rich protein, can be used as feed and soil improvement agent use after oven dry is handled.

Claims

1, amino acid technology is produced in a kind of fermentation, it is characterized in that vinasse, vegetables oil, bicarbonate of ammonia, the potassium primary phosphate back that stirs is added hydrochloric acid, stirs, the accent pH value is PH1-2, and instant pack is fermented, temperature is controlled at 30-40 ℃ in the bag, place after 8-12 days, add NaOH, stir, transfer pH value to PH6, place after 12 hours, carry out solid-liquid separation, must contain the amino acid extracting solution.

2, amino acid technology is produced in a kind of fermentation according to claim 1, it is characterized in that each component content (weight percent): vinasse 94.6-96.5%, vegetables oil 0.65-1.5%, bicarbonate of ammonia 2.1-3.2%, potassium primary phosphate 0.6-0.8%.