CN110823855A - Method for rapidly detecting amphetamine drugs in human urine - Google Patents
Method for rapidly detecting amphetamine drugs in human urine Download PDFInfo
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- CN110823855A CN110823855A CN201911315924.XA CN201911315924A CN110823855A CN 110823855 A CN110823855 A CN 110823855A CN 201911315924 A CN201911315924 A CN 201911315924A CN 110823855 A CN110823855 A CN 110823855A
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
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- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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Abstract
The invention discloses a method for rapidly detecting amphetamine drugs in human urine, which utilizes the selective adsorption of hydrophobic ionic liquid to the amphetamine drugs and utilizes a sulfonyl chloride probe substance to rapidly react with the amphetamine drugs to generate fluorescent substances so as to judge whether the urine contains the amphetamine drugs, wherein the amphetamine drugs comprise: amphetamine, methamphetamine, methylenedioxyamphetamine, methylenedioxymethamphetamine, and the like. The invention fully utilizes the selective adsorption effect of the hydrophobic ionic liquid on the amphetamine drugs in urine to achieve the purpose of enrichment, thereby realizing the effect of high detection limit of the method. The sulfonyl chloride probe substance and the amphetamine drugs are quickly reacted in the hydrophobic ionic liquid to generate the fluorescent substance, so that the characteristics of high accuracy, quick detection, simple operation and easy control of the method are realized.
Description
Technical Field
The invention relates to the field of drug detection, in particular to a method for rapidly detecting amphetamine drugs in human urine.
Background
Smoking and injecting drugs have become a worldwide source of public nuisance, a source of crime to spread white plague. In recent decades, the number of people who take poison is continuously increasing and spreading all over the world, and various unstable factors brought to the society due to poison take have reached the stage of bringing about serious pain to the public, the harm to the public security, the prevalence and spread of infectious diseases caused by the virus take, and the prevalence of venereal diseases and AIDS viruses.
The method is very important for examining related personnel by using a proper analysis and detection means, accurately identifying a drug addict, strengthening social security, carrying out drug contraband work, giving up drugs of the drug addict in time, carrying out tracking investigation on the drug addict, implementing community correction assistant and teaching measures, improving the withdrawal consolidation rate and reducing the relapse rate. However, the existing methods for detecting and identifying drug addicts use devices such as a gas chromatograph and a gas-mass spectrometer. The method has the advantages of high equipment price, complex operation and high detection cost, is difficult to realize on-site rapid detection, and cannot meet the requirements of actual work.
The ionic liquid is a salt composed of anions and cations and is in a liquid state at or near room temperature, and is also called low-temperature molten salt. Ionic liquids are receiving increasing attention from workers in various disciplines due to the incomparable advantages they possess over many conventional solvents. One of the most important characteristics of ionic liquids is their selective dissolution capacity. At present, researchers have utilized this characteristic to separate and enrich multiple substances by different methods.
Disclosure of Invention
In order to solve the problems, the invention provides a method for rapidly detecting amphetamine type drugs in human urine.
In order to achieve the purpose, the invention adopts the technical scheme that:
a method for rapidly detecting amphetamine drugs in human urine utilizes hydrophobic ionic liquid added with a sulfonyl chloride probe substance to detect the amphetamine drugs in the urine.
Furthermore, selective adsorption of the hydrophobic ionic liquid to the phenylalamine narcotic drugs is utilized, and the sulfonyl chloride probe substance and the phenylalamine narcotic drugs are quickly reacted to generate fluorescent substances, so that whether the urine contains the phenylalamine narcotic drugs is judged.
Further, the drugs of amphetamine type include: amphetamine, methamphetamine, methylenedioxyamphetamine, methylenedioxymethamphetamine, and the like.
Further, the hydrophobic ionic liquid is N-alkyl pyridine ionic liquid, alkyl imidazole ionic liquid, functional imidazole ionic liquid, quaternary ammonium salt ionic liquid, pyrrolidine ionic liquid or piperidine ionic liquid.
Further, the method comprises the following steps:
s1, uniformly mixing dichloromethane, hydrophobic ionic liquid and sulfonyl chloride reagent in a mass ratio of 500: 100: 1, making into test paper or test plate, and air drying;
s2, collecting urine sample of the tested person, putting test paper or test board in the urine sample, immersing for 3-5min, and under the irradiation of ultraviolet lamp light, if fluorescence is generated, it indicates that the tested person takes or injects amphetamine drugs, otherwise, it indicates that the tested person does not take or inject amphetamine drugs.
The invention has the following beneficial effects:
1. the selective adsorption effect of the hydrophobic ionic liquid on the amphetamine drugs in urine is fully utilized to achieve the purpose of enrichment, so that the effect of high detection limit of the method is realized.
2. The sulfonyl chloride probe substance and the amphetamine drugs are quickly reacted in the hydrophobic ionic liquid to generate the fluorescent substance, so that the characteristics of high accuracy, quick detection, simple operation and easy control of the method are realized.
3. The method can be made into test paper, test board and the like which are convenient for field use by the processes of coating, dipping, carrying, depositing, grafting and the like, can judge whether the tested personnel is toxic or not on the field, and has strong practicability.
Drawings
FIG. 1 is a chemical structural formula of amphetamine drugs in an embodiment of the present invention.
FIG. 2 is a chemical structural formula of the sulfonyl chloride reagent in the embodiment of the present invention.
Detailed Description
The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that variations and modifications can be made by persons skilled in the art without departing from the spirit of the invention. All falling within the scope of the present invention.
The embodiment of the invention provides a method for rapidly detecting amphetamine drugs in human urine, which utilizes the selective adsorption of hydrophobic ionic liquid on the amphetamine drugs and utilizes the rapid reaction of sulfonyl chloride probe substances and the amphetamine drugs to generate fluorescent substances so as to judge whether the urine contains the amphetamine drugs. The drug of amphetamines comprises: amphetamine, methamphetamine, methylenedioxyamphetamine, methylenedioxymethamphetamine, and the like, and the chemical structural formula is shown in figure 1. The common characteristic of amphetamine drugs can be found from the chemical structural formula in fig. 1 that the structures all contain primary amine or secondary amine groups, which provides an opportunity for finding a chemical analysis method with high accuracy, rapid detection, simple operation and easy mastering.
The hydrophobic ionic liquid used in the invention aims at efficiently and selectively adsorbing amphetamine drugs in urine, and the specific ionic liquid comprises (as shown in table 1):
table 1: hydrophobic ionic liquid species
The sulfonyl chloride probe substance in the invention refers to a sulfonyl chloride reagent which can rapidly react with amphetamine drugs in hydrophobic ionic liquid and generate fluorescent compounds, and specifically includes but is not limited to: 5- (diethylamino) naphthalene-1-sulfonyl chloride, 5- (dimethylamino) naphthalene-2-sulfonyl chloride, 6- (methylamino) naphthalene-2-sulfonyl chloride, 5-ethoxynaphthalene-1-sulfonyl chloride, 6-methoxynaphthalene-2-sulfonyl chloride, anthracene-2-sulfonyl chloride and 4-methoxybenzene-1-sulfonyl chloride, wherein the chemical structural formula is shown in figure 2.
The implementation method of the invention comprises the following steps:
uniformly mixing dichloromethane, hydrophobic ionic liquid and sulfonyl chloride reagent in a mass ratio of 500: 100: 1, preparing test paper or test plate by coating, dipping, carrying, depositing, grafting and other processes, and airing for use. After urine sample of a tested person is collected, test paper, a test plate and the like are put into the urine sample to be soaked for 3-5min, under the irradiation of ultraviolet lamp light, if fluorescence is generated, the tested person inhales or injects the amphetamine drugs, otherwise, the tested person does not inhale or inject the amphetamine drugs.
Verification test of anti-drug interference capability:
after 1mg of common drugs or standard substances are respectively added into 100mL of blank urine, the detection is carried out by the method, and the results are shown in the following table 2:
TABLE 2
The foregoing description of specific embodiments of the present invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes or modifications may be made by one skilled in the art within the scope of the appended claims without departing from the spirit of the invention. The embodiments and features of the embodiments of the present application may be combined with each other arbitrarily without conflict.
Claims (5)
1. A method for rapidly detecting amphetamine drugs in human urine is characterized by comprising the following steps: the hydrophobic ionic liquid added with the sulfonyl chloride probe substance is used for detecting the amphetamine drugs in the urine.
2. The method for rapidly detecting amphetamine-type drugs in human urine as claimed in claim 1, wherein the method comprises the following steps: by utilizing the selective adsorption of the hydrophobic ionic liquid to the phenylpropylamine drugs, the sulfonyl chloride probe substance and the phenylpropylamine drugs are quickly reacted to generate the fluorescent substance, so that whether the urine contains the phenylpropylamine drugs or not is judged.
3. The method for rapidly detecting amphetamine-type drugs in human urine as claimed in claim 1, wherein the method comprises the following steps: the drug of amphetamines comprises: amphetamine, methamphetamine, methylenedioxyamphetamine, methylenedioxymethamphetamine.
4. The method for rapidly detecting amphetamine-type drugs in human urine as claimed in claim 1, wherein the method comprises the following steps: the hydrophobic ionic liquid is N-alkyl pyridine ionic liquid, alkyl imidazole ionic liquid, functional imidazole ionic liquid, quaternary ammonium salt ionic liquid, pyrrolidine ionic liquid or piperidine ionic liquid.
5. The method for rapidly detecting amphetamine-type drugs in human urine as claimed in claim 1, wherein the method comprises the following steps: the method comprises the following steps:
s1, uniformly mixing dichloromethane, hydrophobic ionic liquid and sulfonyl chloride reagent in a mass ratio of 500: 100: 1 to prepare test paper or a test plate, and drying;
s2, collecting urine sample of the tested person, immersing the test paper or the test plate in the urine sample for 3-5min, and under the irradiation of ultraviolet light, if fluorescence is generated, the tested person takes or injects the amphetamine drugs, otherwise, the tested person does not take or inject the amphetamine drugs.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114890958A (en) * | 2022-02-23 | 2022-08-12 | 四川警察学院 | Two-photon dye compound, preparation method and application thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102043052A (en) * | 2009-10-13 | 2011-05-04 | 陈洁 | Rapid detection kit for two items of joint inspection of drug |
| CN104927834A (en) * | 2015-05-11 | 2015-09-23 | 池州学院 | Disubstituted polyacetylene fluorescent dye containing carbazolyl group, and preparation method thereof |
| CN105572241A (en) * | 2014-11-05 | 2016-05-11 | 复旦大学 | Method for assaying amphetamines, ketamine, pethidine and methadone in blood and urine |
| CN106687811A (en) * | 2014-07-15 | 2017-05-17 | C2感官有限公司 | Formulations for enhanced chemiresistive sensing |
| CN109425600A (en) * | 2017-08-30 | 2019-03-05 | 株式会社东芝 | Sensor and reagent |
| CN110327662A (en) * | 2019-04-30 | 2019-10-15 | 云南警官学院 | A kind of Capillary zone electropheresis molecularly imprinted solid phase extraction column and preparation method thereof |
-
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102043052A (en) * | 2009-10-13 | 2011-05-04 | 陈洁 | Rapid detection kit for two items of joint inspection of drug |
| CN106687811A (en) * | 2014-07-15 | 2017-05-17 | C2感官有限公司 | Formulations for enhanced chemiresistive sensing |
| CN105572241A (en) * | 2014-11-05 | 2016-05-11 | 复旦大学 | Method for assaying amphetamines, ketamine, pethidine and methadone in blood and urine |
| CN104927834A (en) * | 2015-05-11 | 2015-09-23 | 池州学院 | Disubstituted polyacetylene fluorescent dye containing carbazolyl group, and preparation method thereof |
| CN109425600A (en) * | 2017-08-30 | 2019-03-05 | 株式会社东芝 | Sensor and reagent |
| CN110327662A (en) * | 2019-04-30 | 2019-10-15 | 云南警官学院 | A kind of Capillary zone electropheresis molecularly imprinted solid phase extraction column and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 唐英章 等: "《保健食品中功效成分及危害物质的分析》", 31 December 2004, 中国标准出版社 * |
| 王军 等: "10-乙基吖啶酮-2-磺酰氯的合成及对甲基苯丙胺的检测", 《化学试剂》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114890958A (en) * | 2022-02-23 | 2022-08-12 | 四川警察学院 | Two-photon dye compound, preparation method and application thereof |
| CN114890958B (en) * | 2022-02-23 | 2023-10-20 | 四川警察学院 | Two-photon dye compound, preparation method and application thereof |
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