CN110804098B - A BK channel light control adjustment element - Google Patents

Abstract

The invention provides a BK channel light-operated regulating element, which comprises a peptide toxin, a light-sensitive protein LOV structural domain, a membrane localization protein and a fluorescent protein. The BK channel light-operated adjusting element has high sensitivity, can realize the regulation and control of an endogenous BK channel, and realizes the precise optical regulation and control of the endogenous BK channel and the regulation and control of subcellular structures such as organelles in cells by utilizing the high time resolution and the high spatial resolution of light.

Description

A BK channel light control adjustment element

technical field

The invention belongs to the field of biotechnology, and in particular relates to a BK channel light control and regulation element.

Background technique

Ion channels are the basis of life activities. There are ion channels on the cell membranes of animals or plants, unicellular organisms or multicellular organisms. The most basic function of ion channels is to generate cellular bioelectric phenomena on excitatory nerve and muscle tissue, which is directly related to the excitability of cells, and the release of neurotransmitters, gland secretion, muscle movement, and even important higher-level neural activities such as learning and memory. Therefore, ion channels are the basic excitatory units on excitable tissue membranes and have extremely important physiological functions. The study of ion channels is directly related to the pathogenesis and drug treatment of ion channel-related diseases.

Large-conductance calcium-activated potassium channel KCa channel is a special type of large-conductance calcium-activated potassium channel (BK). 4～20ps), medium conductance potassium channel (20～80ps). The uniqueness of BK channels lies not only in their large single-channel conductance, but also in that they are dual-regulated by intracellular Ca ²⁺ and membrane depolarization signals. The integration of the two signals makes BK channels a coupled intracellular An important negative feedback system between increased Ca ²⁺ concentration and outward hyperpolarized potassium currents. In addition, signals such as cell membrane stress, cytokines, hormones, ATP, NO, etc. can also activate BK channels.

The main feature of optogenetics technology is the combination of optics and genetics to control the biological functions of neurons through light. Optogenetics was first discovered in the field of neurology and has now been widely used in neural circuits in the brain. The research on neural function regulation mainly uses implantable optical fiber catheters and stereotaxic transfer implantation, so that the corresponding light acts on neurons, and realizes the functions of neuron rapid excitation, rapid inhibition, bistable modulation, and intracellular biochemical information regulation.

As an indispensable tool in optogenetics, many types of photoreceptors have been discovered or synthesized so far, and they have been successfully used in the study of cell function, signal transduction, and molecular function at the subcellular level. Among them, light-oxygen-voltage sensing domain (LOV) domain protein is a kind of photosensitive receptor that has been studied more at present. Its relative molecular mass is small, it is soluble, and its required cofactors exist in most types of photoreceptors. There are multiple ways of regulating signaling molecules in the cells of , these features make LOV an ideal molecule for exploring light-controlled ion channels. Previous studies have incorporated various polypeptides and enzymes into or linked to LOV domains in various ways (LOV regulation).

In neurons and glial cells, ion channels are involved in the regulation of intracellular signals and information integration, and are also important drug targets for many neurological diseases. Gene regulation of ion channels is an ideal model for assessing the role of ion channels in complex nervous systems. There are several strategies for gene regulation of ion channels: one is the expression of genetically encoded membrane-targeted peptide toxins in different types of cells. Peptide toxins are composed of a series of polypeptides genetically encoded from poisonous animals that regulate ion channels, and can specifically recognize various ion channels. These toxins do not require the addition of exogenous chemicals, induce reversible responses over a period of hours to days, and have been shown to function in the living mammalian brain. The second strategy utilizes the modulation of photosensitizing compounds, which are anchored at specific sites of ion channels (eg, SPARK, LiGluR, HyLighter) and can induce rapid and reversible photosensitivity responses. They bind exogenous compounds that bind to target ion channels. The first strategy is completely encoded by genes, but cannot be regulated by light control; the second strategy is regulated by light, but inserts exogenous ion channels and cannot study the function of endogenous ion channels. There are few studies on the regulation of endogenous ion channels by optogenetic means.

SUMMARY OF THE INVENTION

The purpose of the present invention is to provide a BK channel light control regulating element with high sensitivity and capable of effectively regulating the BK channel, aiming at the above technical problems to be solved.

To this end, the present invention provides a BK channel light-controlled regulatory element, which includes a peptide toxin, a light-sensitive protein LOV domain, a membrane-localized protein and a fluorescent protein.

Preferably, the membrane-localized protein is a PDGF (platelet-derived factor) receptor transmembrane protein or a transferrin receptor transmembrane protein. Preferably, the fluorescent protein is mCherry fluorescent protein.

The peptide toxin referred to herein is also called peptide toxin, which is a kind of biologically active substance, and a class of active polypeptides directly encoded by genes. Preferably, the peptide toxin is ibis scorpion toxin or Charybaud scorpion toxin.

Preferably, the N-terminus to the C-terminus of the BK channel light-controlled regulatory element are: peptide toxin, first linker (linker), photosensitive protein LOV domain, second linker, transmembrane protein, mCherry fluorescent protein. The light-sensing protein LOV domain is linked to the second linker through its Jα helix.

Preferably, the N-terminus to the C-terminus of the BK channel light control regulatory element are: peptide toxin, photosensitive protein LOV domain, third linker, transmembrane protein, mCherry fluorescent protein. The light-sensing protein LOV domain is linked to the peptide toxin through its Jα helix.

Preferably, the nucleotide sequences encoding the first linker, the second linker and the third linker are shown in SEQ ID NO.16.

Preferably, the BK channel is an endogenous BK channel.

Preferably, the Jα helix fragment of the light-sensing protein LOV domain is truncated from the 404th amino acid to the 546th amino acid, or the 404th amino acid to the 545th amino acid, or the 404th amino acid to the 544th amino acid, or amino acids 404 to 543, or amino acids 404 to 542, or amino acids 404 to 541, or amino acids 404 to 540, or amino acids 404 to Variants of amino acid 539. Most preferably, the Jα helical fragment of the light-sensing protein LOV domain is truncated as a variant from amino acid 404 to amino acid 539.

The experimental results show that the BK channel light control and adjustment element of the present invention has high sensitivity, can realize the regulation of the endogenous BK channel, and utilizes the high temporal resolution and high spatial resolution of light to realize the precise optical control of the endogenous BK channel. Regulation, as well as the regulation of various intracellular organelles (such as nuclear membrane, mitochondria, etc.) and other subcellular structures.

Description of drawings

FIG. 1 is a schematic diagram of the structure and principle of the BK channel light control and adjustment element of the present invention.

FIG. 2 is a schematic diagram of the electrophysiological results of the BK channel light-controlled regulatory element of the present invention.

FIG. 3 is a schematic diagram of another structural principle of the BK channel light control and adjustment element of the present invention.

Fig. 4 is the electrophysiological result of regulating each variant of BK channel by the BK channel light control regulatory element of the present invention.

Detailed ways

The technical solutions of the present invention will be described in further detail below with reference to the accompanying drawings and specific embodiments, but the present invention is not limited to the following embodiments.

Unless otherwise specified, the reagents mentioned below are all commercially available. For the sake of brevity, some operations do not detail the operation parameters, steps and used instruments, and it should be understood that these are well known and reproducible by those skilled in the art.

1 Materials and methods

1.1 Vectors, cells and strains

Plasmids pcDNA3.1-DTXK-lumitoxin and pmCherry-C1-mTrim21 were purchased from Addgene Company in the United States, with the product numbers on the official website being 51691 and 105516, respectively. Competent DH5α was purchased from Sangon Bioengineering (Shanghai) Co., Ltd., IBTX (Iberiotoxin, Yibi) The nucleotide sequences of scorpion toxin) and CHTX (Charybdotoxin, Charybdotoxin) are shown in SEQ ID NO. 1 and 13, which were synthesized by Sangon Bioengineering (Shanghai) Co., Ltd.

1.2 Main reagents and instruments

Fetal bovine serum, trypsin cell digestion solution, and DMEM medium were purchased from Gibico, USA; Lipofectamine 3000 was purchased from Invitrogen, USA; Ampicillin and nucleic acid dyes were purchased from Saiguo Biological Co., Ltd.; 6×DNA Loading Buffer and DNA marker were purchased from Bao Bio Engineering (Dalian) Co., Ltd.; restriction enzymes NdeI, XholI, Xba I, BamH I were purchased from NEB Company; high-purity plasmid miniprep kit and endotoxin-depleted plasmid extraction kit were purchased from Omega Company.

1.3 Construction of recombinant expression plasmid

1.3.1 Construction of recombinant expression plasmids IBTX-lumitoxin, CHTX-lumitoixn and linker-lumitoxin (the nucleotide sequences of the plasmids are shown in SEQ ID NO.2, SEQ ID NO.3, and SEQ ID NO.4, respectively)

Human-derived IBTX (Iberiotoxin) and CHTX (Charybdotoxin) are followed by a linker sequence. The linker nucleotide sequence is: GGTACCGCCGCCGCCGACTACAAGGACGACGACGACAAGATCGACGCCGCCGCCGGCGGCGCCCTGTGCAAC (as shown in SEQ ID NO. 16), and the HindIII/EcorI enzyme cleavage site is connected at the same time and gene synthesis is performed, The size of the target gene fragments were all 195bp. The synthetic IBTX+linker sequence (as shown in SEQ ID NO. 14) and the CHTX+linker sequence (as shown in SEQ ID NO. 15) were digested by HindIII and EcorI, respectively, and replaced on pcDNA3.1-DTXK-lumitoxin. The DTXK+linker fragment was purified and recovered by nucleic acid electrophoresis, ligated with T4 DNA ligase from Invitrogen for 3 hours, transformed into E.coli DH5α cells, and 8 clones were picked for bacterial liquid PCR verification. The positive clones were expanded and cultured and the plasmid was extracted. IBTX-lumitoxin and CHTX-lumitoixn were obtained, respectively. The recombinant expression plasmids were identified by double enzyme digestion with HindIII and EcorI, and the positive plasmids were sent to Sangon Bioengineering (Shanghai) Co., Ltd. for sequencing. The recombinant bacteria with correct sequencing were expanded and cultured, and the recombinant plasmid was extracted according to the endotoxin-free plasmid extraction kit of Omega Company, and the concentration of the plasmid was determined by NanoDrop One ultra-micro spectrophotometer.

HiFi DNA Assembly无缝克隆试剂盒去除IBTX-lumitoxin中的IBTX基因序列，构建成linker-lumitoxin重组质粒，引物序列如下：正向引物5’-3’：ggacgacgacgacaagctgaagcttGGTACCGCCGCCGCCGAC(SEQ ID NO.17)，反向引物5’-3’：AAGCTTCAGCTTGTCGTCGTCGTCCTTG(SEQ ID NO.18)。同理，新构建linker-lumitoxin质粒经过PCR、双酶切验证和测序后，阳性克隆扩大培养并用无内毒素质提取试剂盒提取重组质粒。use

HiFi DNA Assembly Seamless Cloning Kit removes the IBTX gene sequence in IBTX-lumitoxin and constructs a linker-lumitoxin recombinant plasmid. The primer sequences are as follows: Forward primer 5'-3': ggacgacgacgacaagctgaagcttGGTACCGCCGCCGCCGAC (SEQ ID NO.17), reverse Primers 5'-3': AAGCTCAGCTTGTCGTCGTCGTCCTTG (SEQ ID NO. 18). Similarly, after the newly constructed linker-lumitoxin plasmid was verified by PCR, double-enzyme digestion and sequencing, the positive clones were expanded and cultured, and the recombinant plasmid was extracted with an endotoxin-free extraction kit.

1.3.2 IBTX light-controlled regulation of BK channel plasmid construction—Optogenetic control of ion channel blocking (OCIB, optogenetic control of ion channel blocking) (the nucleotide sequences of the plasmids are shown in SEQ ID NO.5-12, respectively)

Using pmCherry-C1-mTrim21 as the vector plasmid and using the principle of seamless cloning, primers were designed to remove the open reading frame mCherry post-sequence, and insert the transferrin receptor transmembrane segment and IBTX sequence at the same time.

The primer sequences are as follows:

Vector forward primer 5'-3': GCGGCCGCTCTAGATAAC (SEQ ID NO. 19),

Vector reverse primer 5'-3': TGAATTCGAAGCTTGAGCTC (SEQ ID NO.20),

transferrinR insert forward primer: atctcgagctcaagcttcgaattcaATGATGGATCAAGCCAGGAG (SEQ ID NO. 21),

transferrinR insert reverse primer:

gctgccgccgccgccgctgccgccgccgccACAGTAGCCCAGGTAGCC (SEQ ID NO. 22),

IBTX insert forward primer:

ggcggcggcggcagcggcggcggcggcagcCAGTTTACAGATGTGGATTG (SEQ ID NO. 23),

IBTX insert reverse primers 5'-3': atgatcagttatctagagcggccgcCTACTGGTAGCATCTACAC (SEQ ID NO. 24).

HiFi DNA Assembly无缝克隆试剂盒同时连接载体和transferrin受体(transferrinR)和IBTX插入片段。连接产物转化E.coli DH5α细胞，挑取8个克隆进行菌液PCR验证，阳性克隆送生工生物工程(上海)股份有限公司测序，测序正确克隆扩大培养后提取质粒。PCR amplification was carried out using KOD DNA polymerase of TOYOBO company, the PCR product was electrophoresed on agarose, and the Omega kit was used to cut the gel for recovery.

The HiFi DNA Assembly Seamless Cloning Kit ligates the vector and transferrin receptor (transferrinR) and IBTX insert simultaneously. The ligation product was transformed into E.coli DH5α cells, and 8 clones were picked for bacterial liquid PCR verification. The positive clones were sent to Sangon Bioengineering (Shanghai) Co., Ltd. for sequencing.

Subsequently, LOV fragments of different lengths (from the 404th amino acid to the 546th amino acid to the 404th amino acid to the 539th amino acid) were inserted into the above vector, and the primer sequences were as follows:

Vector forward primer: CAGTTTACAGATGTGGATTGCAGCGTGAGCAAGGAGTGCTGGAG (SEQ ID NO. 25),

Vector reverse primer: GCTGCCGCCGCCGCCGCT (SEQ ID NO. 26),

LOV fragment universal forward primer: cggcggcagcggcggcggcggcagcTTGGCTACTACACTTGAAC (SEQID NO.27),

LOV 546 fragment reverse primer: cgctgcaatccacatctgtaaactgAAGTTCTTTTGCCGCCTC (SEQID NO.28),

LOV 545 fragment reverse primer: cgctgcaatccacatctgtaaactgTTCTTTTGCCGCCTCATC (SEQ ID NO. 29),

LOV 544 fragment reverse primer: cgctgcaatccacatctgtaaactgTTTTGCCGCCTCATCAATATTTTC (SEQ ID NO. 30),

LOV 543 fragment reverse primer: cgctgcaatccacatctgtaaactgTGCCGCCTCATCAATATTTTC (SEQ ID NO. 31),

LOV 542 fragment reverse primer: cgctgcaatccacatctgtaaactgCGCCTCATCAATATTTTCTG (SEQID NO.32),

LOV 541 fragment reverse primer: cgctgcaatccacatctgtaaactgCTCATCAATATTTTCTGCAG (SEQ ID NO. 33),

LOV 540 fragment reverse primer: cgctgcaatccacatctgtaaactgATCAATATTTTCTGCAGTTTTC (SEQ ID NO. 34),

LOV 539 fragment reverse primer: cgctgcaatccacatctgtaaactgAATATTTTCTGCAGTTTTCTTAATC (SEQ ID NO. 35).

HiFi DNA Assembly无缝克隆试剂盒分别连接载体和不同长度的LOV插入片段。所得产物质粒的核苷酸序列分别如SEQ ID NO.5(对应LOV404-546)、SEQID NO.6(对应LOV 404-545)、SEQ ID NO.7(对应LOV 404-544)、SEQ ID NO.8(对应LOV404-543)、SEQ ID NO.9(对应LOV 404-542)、SEQ ID NO.10(对应LOV 404-541)、SEQ IDNO.11(对应LOV 404-540)、SEQ ID NO.12(对应LOV 404-539)所示。连接产物转化E.coliDH5α细胞，随机挑取8个克隆进行菌液PCR验证，阳性克隆送生工生物工程(上海)股份有限公司测序，测序正确克隆扩大培养后提取质粒。PCR amplification was carried out using KOD DNA polymerase of TOYOBO company, the PCR product was electrophoresed on agarose, and the Omega kit was used to cut the gel for recovery.

HiFi DNA Assembly Seamless Cloning Kit ligates the vector and LOV inserts of different lengths respectively. The nucleotide sequences of the resulting product plasmids are respectively as SEQ ID NO.5 (corresponding to LOV404-546), SEQ ID NO.6 (corresponding to LOV 404-545), SEQ ID NO.7 (corresponding to LOV 404-544), SEQ ID NO. .8 (corresponding to LOV404-543), SEQ ID NO.9 (corresponding to LOV404-542), SEQ ID NO.10 (corresponding to LOV404-541), SEQ ID NO.11 (corresponding to LOV404-540), SEQ ID NO. .12 (corresponding to LOV 404-539). The ligation product was transformed into E.coliDH5α cells, and 8 clones were randomly selected for bacterial liquid PCR verification. The positive clones were sent to Sangon Bioengineering (Shanghai) Co., Ltd. for sequencing.

1.4 Transient transfection and expression

The above recombinant plasmids were respectively transfected into HEK 293T cells with lip3000 of Invitrogen Company, and the BK channel plasmids were co-transfected at the same time. Lip3000 liposome transfection step: HEK293T cells were seeded into 24-well plates and transfected when they grew to 80% adherent.

Tube A: Dilute 1 μL Lipofectamine ^TM 3000 reagent with 25 μL Opti-MEM ^TM medium and mix well.

Tube B: Add 25 μL Opti-MEM ^TM medium to 0.5 μg DNA, add 1 μL P3000 ^TM reagent, and mix well. After mixing tube A and tube B 1:1, incubate at room temperature for 15 min. Add 50 μL of DNA-lipid complex to each well of cells. Put it back in the 37°C incubator, and do electrophysiological experiments 24 hours later.

1.5 Patch clamp experimental steps:

1.5.1 Preparation of microelectrodes: Pull the electrode to about 10mm in length and less than 0.2mm in diameter, and use a pulling electrode instrument

1.5.2 Set the procedure for the second drawing, so that the final use diameter of the tip is 1-2 μm. Coat the front of the electrode with silicone resin to reduce the capacitance between the electrode and the perfusate. Pour the electrode solution into the electrode: first immerse the electrode tip in the inner solution for about 5s, then insert a polypropylene syringe from the back end of the electrode to the vicinity of the tip, fill the solution to 1/4 of the length, and eject the air bubbles at the tip.

1.5.3 Put the electrode into contact with the cell membrane, first suck gently to form a high-resistance seal between the microelectrode and the cell membrane, and the resistance reaches more than 1GΩ, and then continue to suck hard to break the cell membrane, so that the liquid in the electrode communicates with the liquid in the cell, which can be recorded. Whole cell potential. When the high-resistance seal is formed, the electrode is gently withdrawn, and a small piece of cell membrane can be torn off, and the transmembrane ionic current on this piece of membrane can be recorded. If there is only one ion channel, a single-channel current can be obtained.

When recording single-channel data, clamp the membrane potential at -70mV, and after the current stabilizes, irradiate blue light with a wavelength of 450nm for 20s, and record the current amplitude change with gapfree.

When recording whole-cell data, clamp the membrane potential at -70mV, then depolarize the stimulus to +40mV for 300ms, and then irradiate with 450nm wavelength blue light for 20s.

2. Results

2.1 Electrophysiological results of IBTX light-regulated BK channel plasmids

Figure 1 is a schematic diagram of the structural principle of the IBTX light-regulated BK channel plasmid OCIB. The N-terminus to C-terminus of the plasmid are: IBTX peptide toxin, linker, photosensitive protein LOV domain and Jα helix, linker, PDGF transmembrane segment, mCherry fluorescent protein. As shown, the BK ion channel is an endogenous BK channel.

In the dark state, the IBTX peptide toxin specifically binds to BK channels and reduces K ion efflux. Under the irradiation of 450nm blue light, the LOV domain is photosensitive, and the conformation of Jα changes, which increases the IBTX peptide toxin, IBTX cannot block the BK channel, and the outflow of K ions increases.

According to the above structure, three light-controlling BK channel plasmids were prepared: negative control linker-lumitoxin, CHTX-lumitoxin, and IBTX-lumitoxin. IBTX is Iberiotoxin, and CHTX is charybdotoxin.

Figure 2 shows the electrophysiological results of CHTX-lumitoxin, IBTX-lumitoxin and negative control linker-lumitoxin transfecting HEK293T cells before and after blue light (n=6). After blue light, the current of IBTX-lumitoxin and CHTX-lumitoxinBK increased. After the light is removed, the current decreases. This shows that both CHTX-lumitoxin and IBTX-lumitoxin have the function of regulating BK channel.

Figure 3 is a schematic diagram of the structural principle of another IBTX light-regulated BK channel plasmid, OCIB. As shown in the figure, the N-terminus to the C-terminus of the plasmid are: IBTX peptide toxin, Jα helix and photosensitive protein LOV domain, linker, transferrin receptor transmembrane segment, mCherry fluorescent protein. Among them, the BK ion channel is an endogenous BK channel.

In the dark state, the IBTX peptide toxin specifically binds to BK channels and reduces K ion efflux. Under the irradiation of 450nm blue light, the LOV domain is photosensitive, and the conformation of Jα changes, which increases the IBTX peptide toxin, IBTX cannot block the BK channel, and the outflow of K ions increases.

According to the above structure, three light-controlling BK channel plasmids were prepared: negative control linker-lumitoxin, CHTX-lumitoxin, and IBTX-lumitoxin. IBTX is Iberiotoxin, and CHTX is charybdotoxin. Electrophysiological results of HEK293T cells transfected with CHTX-lumitoxin, IBTX-lumitoxin and negative control linker-lumitoxin before and after blue light exposure (n=6). After blue light exposure, IBTX-lumitoxin and CHTX-lumitoxin BK currents increased. After the light is removed, the current decreases. This shows that both CHTX-lumitoxin and IBTX-lumitoxin have the function of regulating BK channel.

In order to find the variant that best regulates BK current, LOV-Jα was truncated into different variants, truncated from the 404th amino acid to the 546th amino acid to the 404th amino acid to the 539th amino acid, and transfected with 293T blue light After irradiation, electrophysiological experiments found that variants 539 (that is, the fragment was from amino acids 404 to 539), 540 (that is, the fragment was from amino acids 404 to 540), and 541 (that was, the fragment was from amino acids 404) amino acid to amino acid 541), 545 (that is, the fragment is amino acid 404 to amino acid 545), and 546 (that is, the fragment is amino acid 404 to amino acid 546), all increase the BK current, among which 539 increases the BK current. The BK current increased the most (n=5). Figure 4 shows the electrophysiological results of IBTX regulating each variant of BK channel.

sequence listing

<110> Sun Yat-Sen University

<120> A BK channel light control element

<160> 35

<170> SIPOSequenceListing 1.0

<210> 1

<211> 111

<212> DNA

<213> Artificial Sequence

<400> 1

cagtttacag atgtggattg cagcgtgagc aaggagtgct ggagcgtgtg caaggatctg 60

tttggagtgg atagaggaaa gtgcatgggc aagaagtgta gatgctacca g 111

<210> 2

<211> 6925

<212> DNA

<213> Artificial Sequence

<400> 2

gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

atgagcgccc tgctgatcct ggccctggtg ggcgccgccg tggccgacta caaggacgac 960

gacgacaagc tgaagcttca gtttacagat gtggattgca gcgtgagcaa ggagtgctgg 1020

agcgtgtgca aggatctgtt tggagtggat agaggaaagt gcatgggcaa gaagtgtaga 1080

tgctaccagg gtaccgccgc cgccgactac aaggacgacg acgacaagat cgacgccgcc 1140

gccggcggcg ccctgtgcaa cgaattcttg gctactacac ttgaacgtat tgagaagaac 1200

tttgtcatta ctgacccaag attgccagat aatcccatta tattcgcgtc cgatagtttc 1260

ttgcagttga cagaatatag ccgtgaagaa attttgggaa gaaactgcag gtttctacaa 1320

ggtcctgaaa ctgatcgcgc gacagtgaga aaaattagag atgccataga taaccaaaca 1380

gaggtcactg ttcagctgat taattataca aagagtggta aaaagttctg gaacctcttt 1440

cacttgcagc ctatgcgaga tcagaaggga gatgtccagt actttattgg ggttcagttg 1500

gatggaactg agcatgtccg agatgctgcc gagagagagg gagtcatgct gattaagaaa 1560

actgcagaaa atattgatga ggcggcaaaa gaacttagcg gccgcggtac tcgagttgct 1620

gtgggccagg acacgcagga ggtcatcgtg gtgccacact ccttgccctt taaggtggtg 1680

gtgatctcag ccatcctggc cctggtggtg ctcaccatca tctcccttat catcctcatc 1740

atgctttggc agaagaaacc acgtaggatt cgtatggtga gcaagggcga ggaggataac 1800

atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc 1860

cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc 1920

aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag 1980

ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag 2040

ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg 2100

gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg 2160

cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag 2220

gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg 2280

ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag 2340

aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac 2400

aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc 2460

ggcatggacg agctgtacaa gtaatctaga gggcccgttt aaacccgctg atcagcctcg 2520

actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc 2580

ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt 2640

ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat 2700

tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa 2760

agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg 2820

gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 2880

cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 2940

aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 3000

cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 3060

ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 3120

aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg 3180

ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc 3240

agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 3300

tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 3360

aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 3420

ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 3480

atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt 3540

ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg 3600

atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt 3660

ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct 3720

gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga 3780

ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg 3840

ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact 3900

ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg 3960

agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct 4020

gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg 4080

gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt 4140

tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg 4200

cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc 4260

ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag 4320

agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt 4380

cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt 4440

cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc 4500

cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca 4560

gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa 4620

tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 4680

ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac 4740

ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 4800

gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 4860

atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 4920

cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 4980

tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 5040

agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 5100

aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 5160

gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 5220

tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 5280

cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 5340

ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 5400

cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 5460

atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 5520

agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 5580

gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 5640

gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 5700

tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 5760

agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 5820

gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 5880

aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 5940

aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 6000

ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 6060

gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 6120

aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 6180

ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 6240

tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 6300

ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 6360

cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 6420

agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 6480

gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 6540

gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 6600

acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 6660

acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 6720

agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 6780

aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 6840

gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 6900

tccccgaaaa gtgccacctg acgtc 6925

<210> 3

<211> 6925

<212> DNA

<213> Artificial Sequence

<400> 3

gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

atgagcgccc tgctgatcct ggccctggtg ggcgccgccg tggccgacta caaggacgac 960

gacgacaagc tgaagcttca gtttacaaac gtcagctgca caacaagcaa ggagtgctgg 1020

agcgtgtgcc agagactgca caacacaagc agaggaaagt gcatgaacaa gaagtgcaga 1080

tgctacagcg gtaccgccgc cgccgactac aaggacgacg acgacaagat cgacgccgcc 1140

gccggcggcg ccctgtgcaa cgaattcttg gctactacac ttgaacgtat tgagaagaac 1200

tttgtcatta ctgacccaag attgccagat aatcccatta tattcgcgtc cgatagtttc 1260

ttgcagttga cagaatatag ccgtgaagaa attttgggaa gaaactgcag gtttctacaa 1320

ggtcctgaaa ctgatcgcgc gacagtgaga aaaattagag atgccataga taaccaaaca 1380

gaggtcactg ttcagctgat taattataca aagagtggta aaaagttctg gaacctcttt 1440

cacttgcagc ctatgcgaga tcagaaggga gatgtccagt actttattgg ggttcagttg 1500

gatggaactg agcatgtccg agatgctgcc gagagagagg gagtcatgct gattaagaaa 1560

actgcagaaa atattgatga ggcggcaaaa gaacttagcg gccgcggtac tcgagttgct 1620

gtgggccagg acacgcagga ggtcatcgtg gtgccacact ccttgccctt taaggtggtg 1680

gtgatctcag ccatcctggc cctggtggtg ctcaccatca tctcccttat catcctcatc 1740

atgctttggc agaagaaacc acgtaggatt cgtatggtga gcaagggcga ggaggataac 1800

atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc 1860

cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc 1920

aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag 1980

ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag 2040

ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg 2100

gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg 2160

cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag 2220

gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg 2280

ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag 2340

aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac 2400

aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc 2460

ggcatggacg agctgtacaa gtaatctaga gggcccgttt aaacccgctg atcagcctcg 2520

actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc 2580

ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt 2640

ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat 2700

tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa 2760

agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg 2820

gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 2880

cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 2940

aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 3000

cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 3060

ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 3120

aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg 3180

ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc 3240

agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 3300

tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 3360

aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 3420

ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 3480

atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt 3540

ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg 3600

atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt 3660

ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct 3720

gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga 3780

ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg 3840

ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact 3900

ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg 3960

agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct 4020

gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg 4080

gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt 4140

tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg 4200

cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc 4260

ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag 4320

agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt 4380

cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt 4440

cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc 4500

cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca 4560

gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa 4620

tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 4680

ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac 4740

ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 4800

gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 4860

atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 4920

cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 4980

tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 5040

agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 5100

aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 5160

gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 5220

tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 5280

cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 5340

ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 5400

cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 5460

atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 5520

agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 5580

gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 5640

gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 5700

tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 5760

agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 5820

gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 5880

aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 5940

aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 6000

ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 6060

gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 6120

aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 6180

ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 6240

tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 6300

ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 6360

cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 6420

agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 6480

gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 6540

gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 6600

acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 6660

acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 6720

agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 6780

aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 6840

gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 6900

tccccgaaaa gtgccacctg acgtc 6925

<210> 4

<211> 6814

<212> DNA

<213> Artificial Sequence

<400> 4

gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

atgagcgccc tgctgatcct ggccctggtg ggcgccgccg tggccgacta caaggacgac 960

gacgacaagc tgaagcttgg taccgccgcc gccgactaca aggacgacga cgacaagatc 1020

gacgccgccg ccggcggcgc cctgtgcaac gaattcttgg ctactacact tgaacgtatt 1080

gagaagaact ttgtcattac tgacccaaga ttgccagata atcccattat attcgcgtcc 1140

gatagtttct tgcagttgac agaatatagc cgtgaagaaa ttttgggaag aaactgcagg 1200

tttctacaag gtcctgaaac tgatcgcgcg acagtgagaa aaattagaga tgccatagat 1260

aaccaaacag aggtcactgt tcagctgatt aattatacaa agagtggtaa aaagttctgg 1320

aacctctttc acttgcagcc tatgcgagat cagaagggag atgtccagta ctttattggg 1380

gttcagttgg atggaactga gcatgtccga gatgctgccg agagagaggg agtcatgctg 1440

attaagaaaa ctgcagaaaa tattgatgag gcggcaaaag aacttagcgg ccgcggtact 1500

cgagttgctg tgggccagga cacgcaggag gtcatcgtgg tgccacactc cttgcccttt 1560

aaggtggtgg tgatctcagc catcctggcc ctggtggtgc tcaccatcat ctcccttatc 1620

atcctcatca tgctttggca gaagaaacca cgtaggattc gtatggtgag caagggcgag 1680

gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc 1740

gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc 1800

cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg 1860

tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac 1920

tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac 1980

ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag 2040

gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg 2100

ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc 2160

aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac 2220

aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc 2280

acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac 2340

tccaccggcg gcatggacga gctgtacaag taatctagag ggcccgttta aacccgctga 2400

tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct 2460

tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca 2520

tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag 2580

ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct 2640

gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca 2700

ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 2760

gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 2820

caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 2880

cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 2940

tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 3000

acaacactca accctatctc ggtctattct tttgattt aagggatttt gccgatttcg 3060

gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga 3120

atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 3180

gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 3240

gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 3300

ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 3360

ttttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag 3420

gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt 3480

tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc 3540

acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 3600

caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 3660

ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat 3720

cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 3780

gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 3840

ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 3900

cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 3960

tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 4020

ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc 4080

atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 4140

actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 4200

ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 4260

ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac 4320

tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc 4380

caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 4440

gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc 4500

agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 4560

ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat 4620

accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 4680

ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 4740

gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 4800

gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 4860

tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 4920

gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 4980

ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 5040

ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 5100

acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 5160

tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 5220

ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 5280

ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 5340

ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 5400

actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 5460

gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 5520

tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 5580

caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 5640

atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 5700

acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 5760

ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 5820

ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 5880

tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 5940

tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 6000

gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 6060

tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 6120

tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 6180

ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 6240

tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 6300

ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 6360

gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 6420

ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 6480

cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 6540

ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 6600

ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 6660

gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 6720

ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 6780

gcgcacattt ccccgaaaag tgccacctga cgtc 6814

<210> 5

<211> 5520

<212> DNA

<213> Artificial Sequence

<400> 5

cagtttacag atgtggattg cagcgtgagc aaggagtgct ggagcgtgtg caaggatctg 60

tttggagtgg atagaggaaa gtgcatgggc aagaagtgta gatgctacca gtagtctaga 120

taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc 180

ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 240

attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 300

tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta 360

aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 420

ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 480

agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 540

cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 600

atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 660

ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 720

gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 780

acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt 840

gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 900

acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag 960

aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 1020

agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 1080

tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 1140

cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 1200

ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 1260

cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac 1320

aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 1380

ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 1440

cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 1500

cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 1560

cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 1620

gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 1680

catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 1740

ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 1800

tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 1860

caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 1920

gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 1980

ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 2040

cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 2100

cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 2160

gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 2220

aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 2280

ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca 2340

ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt 2400

gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca 2460

ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca 2520

agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc 2580

tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 2640

taggtgaaga tccttttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 2700

cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc ttttttttctg 2760

cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 2820

gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 2880

aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 2940

cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 3000

tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 3060

acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 3120

ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 3180

ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 3240

tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 3300

tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 3360

ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 3420

gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt 3480

tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 3540

accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 3600

aatagggact ttccattgac gtcaatgggt ggattatta cggtaaactg cccacttggc 3660

agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 3720

gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 3780

ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg 3840

tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag 3900

tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt 3960

gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt 4020

gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggaggat 4080

aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac 4140

ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc 4200

gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct 4260

cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg 4320

aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc 4380

gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag 4440

ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg 4500

gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag 4560

aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc 4620

aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc 4680

cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc 4740

ggcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc ttcgatgatg 4800

gatcaagcca ggagcgcctt cagcaatctg ttcggcggag agcctctgag ctacaccaga 4860

ttcagcctgg ccaggcaagt ggatggcgat aacagccacg tggagatgaa gctggctgtg 4920

gacgaagaag agaacgccga caacaacacc aaggccaacg tgaccaagcc taagagatgc 4980

agcggcagca tctgctatgg aaccatcgcc gtgatcgtgt tcttcctgat cggcttcatg 5040

atcggctacc tgggctactg tggcggcggc ggcagcggcg gcggcggcag cttggctact 5100

acacttgaac gtattgagaa gaactttgtc attactgacc caagattgcc agataatccc 5160

attattattcg cgtccgatag tttcttgcag ttgacagaat atagccgtga agaaattttg 5220

ggaagaaact gcaggtttct acaaggtcct gaaactgatc gcgcgacagt gagaaaaatt 5280

agagatgcca tagataacca aacagaggtc actgttcagc tgattaatta tacaaagagt 5340

ggtaaaaagt tctggaacct ctttcacttg cagcctatgc gagatcagaa gggagatgtc 5400

cagtacttta ttggggttca gttggatgga actgagcatg tccgagatgc tgccgagaga 5460

gagggagtca tgctgattaa gaaaactgca gaaaatattg atgaggcggc aaaagaactt 5520

<210> 6

<211> 5517

<212> DNA

<213> Artificial Sequence

<400> 6

cagtttacag atgtggattg cagcgtgagc aaggagtgct ggagcgtgtg caaggatctg 60

tttggagtgg atagaggaaa gtgcatgggc aagaagtgta gatgctacca gtagtctaga 120

taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc 180

ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 240

attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 300

tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta 360

aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 420

ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 480

agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 540

cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 600

atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 660

ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 720

gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 780

acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt 840

gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 900

acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag 960

aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 1020

agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 1080

tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 1140

cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 1200

ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 1260

cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac 1320

aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 1380

ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 1440

cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 1500

cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 1560

cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 1620

gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 1680

catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 1740

ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 1800

tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 1860

caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 1920

gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 1980

ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 2040

cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 2100

cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 2160

gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 2220

aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 2280

ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca 2340

ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt 2400

gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca 2460

ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca 2520

agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc 2580

tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 2640

taggtgaaga tccttttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 2700

cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc ttttttttctg 2760

cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 2820

gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 2880

aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 2940

cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 3000

tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 3060

acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 3120

ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 3180

ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 3240

tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 3300

tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 3360

ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 3420

gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt 3480

tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 3540

accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 3600

aatagggact ttccattgac gtcaatgggt ggattatta cggtaaactg cccacttggc 3660

agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 3720

gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 3780

ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg 3840

tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag 3900

tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt 3960

gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt 4020

gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggaggat 4080

aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac 4140

ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc 4200

gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct 4260

cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg 4320

aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc 4380

gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag 4440

ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg 4500

gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag 4560

aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc 4620

aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc 4680

cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc 4740

ggcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc ttcgatgatg 4800

gatcaagcca ggagcgcctt cagcaatctg ttcggcggag agcctctgag ctacaccaga 4860

ttcagcctgg ccaggcaagt ggatggcgat aacagccacg tggagatgaa gctggctgtg 4920

gacgaagaag agaacgccga caacaacacc aaggccaacg tgaccaagcc taagagatgc 4980

agcggcagca tctgctatgg aaccatcgcc gtgatcgtgt tcttcctgat cggcttcatg 5040

atcggctacc tgggctactg tggcggcggc ggcagcggcg gcggcggcag cttggctact 5100

acacttgaac gtattgagaa gaactttgtc attactgacc caagattgcc agataatccc 5160

attattattcg cgtccgatag tttcttgcag ttgacagaat atagccgtga agaaattttg 5220

ggaagaaact gcaggtttct acaaggtcct gaaactgatc gcgcgacagt gagaaaaatt 5280

agagatgcca tagataacca aacagaggtc actgttcagc tgattaatta tacaaagagt 5340

ggtaaaaagt tctggaacct ctttcacttg cagcctatgc gagatcagaa gggagatgtc 5400

cagtacttta ttggggttca gttggatgga actgagcatg tccgagatgc tgccgagaga 5460

gagggagtca tgctgattaa gaaaactgca gaaaatattg atgaggcggc aaaagaa 5517

<210> 7

<211> 5514

<212> DNA

<213> Artificial Sequence

<400> 7

cagtttacag atgtggattg cagcgtgagc aaggagtgct ggagcgtgtg caaggatctg 60

tttggagtgg atagaggaaa gtgcatgggc aagaagtgta gatgctacca gtagtctaga 120

taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc 180

ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 240

attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 300

tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta 360

aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 420

ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 480

agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 540

cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 600

atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 660

ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 720

gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 780

acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt 840

gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 900

acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag 960

aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 1020

agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 1080

tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 1140

cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 1200

ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 1260

cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac 1320

aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 1380

ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 1440

cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 1500

cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 1560

cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 1620

gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 1680

catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 1740

ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 1800

tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 1860

caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 1920

gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 1980

ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 2040

cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 2100

cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 2160

gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 2220

aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 2280

ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca 2340

ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt 2400

gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca 2460

ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca 2520

agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc 2580

tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 2640

taggtgaaga tccttttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 2700

cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc ttttttttctg 2760

cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 2820

gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 2880

aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 2940

cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 3000

tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 3060

acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 3120

ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 3180

ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 3240

tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 3300

tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 3360

ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 3420

gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt 3480

tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 3540

accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 3600

aatagggact ttccattgac gtcaatgggt ggattatta cggtaaactg cccacttggc 3660

agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 3720

gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 3780

ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg 3840

tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag 3900

tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt 3960

gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt 4020

gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggaggat 4080

aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac 4140

ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc 4200

gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct 4260

cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg 4320

aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc 4380

gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag 4440

ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg 4500

gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag 4560

aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc 4620

aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc 4680

cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc 4740

ggcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc ttcgatgatg 4800

gatcaagcca ggagcgcctt cagcaatctg ttcggcggag agcctctgag ctacaccaga 4860

ttcagcctgg ccaggcaagt ggatggcgat aacagccacg tggagatgaa gctggctgtg 4920

gacgaagaag agaacgccga caacaacacc aaggccaacg tgaccaagcc taagagatgc 4980

agcggcagca tctgctatgg aaccatcgcc gtgatcgtgt tcttcctgat cggcttcatg 5040

atcggctacc tgggctactg tggcggcggc ggcagcggcg gcggcggcag cttggctact 5100

acacttgaac gtattgagaa gaactttgtc attactgacc caagattgcc agataatccc 5160

attattattcg cgtccgatag tttcttgcag ttgacagaat atagccgtga agaaattttg 5220

ggaagaaact gcaggtttct acaaggtcct gaaactgatc gcgcgacagt gagaaaaatt 5280

agagatgcca tagataacca aacagaggtc actgttcagc tgattaatta tacaaagagt 5340

ggtaaaaagt tctggaacct ctttcacttg cagcctatgc gagatcagaa gggagatgtc 5400

cagtacttta ttggggttca gttggatgga actgagcatg tccgagatgc tgccgagaga 5460

gagggagtca tgctgattaa gaaaactgca gaaaatattg atgaggcggc aaaa 5514

<210> 8

<211> 5511

<212> DNA

<213> Artificial Sequence

<400> 8

cagtttacag atgtggattg cagcgtgagc aaggagtgct ggagcgtgtg caaggatctg 60

tttggagtgg atagaggaaa gtgcatgggc aagaagtgta gatgctacca gtagtctaga 120

taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc 180

ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 240

attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 300

tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta 360

aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 420

ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 480

agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 540

cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 600

atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 660

ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 720

gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 780

acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt 840

gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 900

acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag 960

aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 1020

agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 1080

tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 1140

cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 1200

ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 1260

cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac 1320

aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 1380

ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 1440

cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 1500

cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 1560

cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 1620

gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 1680

catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 1740

ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 1800

tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 1860

caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 1920

gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 1980

ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 2040

cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 2100

cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 2160

gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 2220

aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 2280

ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca 2340

ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt 2400

gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca 2460

ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca 2520

agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc 2580

tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 2640

taggtgaaga tccttttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 2700

cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc ttttttttctg 2760

cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 2820

gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 2880

aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 2940

cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 3000

tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 3060

acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 3120

ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 3180

ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 3240

tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 3300

tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 3360

ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 3420

gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt 3480

tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 3540

accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 3600

aatagggact ttccattgac gtcaatgggt ggattatta cggtaaactg cccacttggc 3660

agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 3720

gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 3780

ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg 3840

tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag 3900

tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt 3960

gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt 4020

gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggaggat 4080

aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac 4140

ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc 4200

gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct 4260

cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg 4320

aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc 4380

gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag 4440

ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg 4500

gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag 4560

aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc 4620

aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc 4680

cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc 4740

ggcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc ttcgatgatg 4800

gatcaagcca ggagcgcctt cagcaatctg ttcggcggag agcctctgag ctacaccaga 4860

ttcagcctgg ccaggcaagt ggatggcgat aacagccacg tggagatgaa gctggctgtg 4920

gacgaagaag agaacgccga caacaacacc aaggccaacg tgaccaagcc taagagatgc 4980

agcggcagca tctgctatgg aaccatcgcc gtgatcgtgt tcttcctgat cggcttcatg 5040

atcggctacc tgggctactg tggcggcggc ggcagcggcg gcggcggcag cttggctact 5100

acacttgaac gtattgagaa gaactttgtc attactgacc caagattgcc agataatccc 5160

attattattcg cgtccgatag tttcttgcag ttgacagaat atagccgtga agaaattttg 5220

ggaagaaact gcaggtttct acaaggtcct gaaactgatc gcgcgacagt gagaaaaatt 5280

agagatgcca tagataacca aacagaggtc actgttcagc tgattaatta tacaaagagt 5340

ggtaaaaagt tctggaacct ctttcacttg cagcctatgc gagatcagaa gggagatgtc 5400

cagtacttta ttggggttca gttggatgga actgagcatg tccgagatgc tgccgagaga 5460

gagggagtca tgctgattaa gaaaactgca gaaaatattg atgaggcggc a 5511

<210> 9

<211> 5508

<212> DNA

<213> Artificial Sequence

<400> 9

cagtttacag atgtggattg cagcgtgagc aaggagtgct ggagcgtgtg caaggatctg 60

tttggagtgg atagaggaaa gtgcatgggc aagaagtgta gatgctacca gtagtctaga 120

taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc 180

ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 240

attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 300

tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta 360

aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 420

ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 480

agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 540

cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 600

atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 660

ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 720

gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 780

acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt 840

gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 900

acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag 960

aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 1020

agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 1080

tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 1140

cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 1200

ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 1260

cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac 1320

aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 1380

ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 1440

cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 1500

cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 1560

cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 1620

gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 1680

catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 1740

ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 1800

tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 1860

caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 1920

gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 1980

ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 2040

cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 2100

cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 2160

gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 2220

aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 2280

ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca 2340

ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt 2400

gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca 2460

ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca 2520

agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc 2580

tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 2640

taggtgaaga tccttttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 2700

cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc ttttttttctg 2760

cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 2820

gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 2880

aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 2940

cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 3000

tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 3060

acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 3120

ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 3180

ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 3240

tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 3300

tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 3360

ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 3420

gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt 3480

tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 3540

accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 3600

aatagggact ttccattgac gtcaatgggt ggattatta cggtaaactg cccacttggc 3660

agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 3720

gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 3780

ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg 3840

tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag 3900

tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt 3960

gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt 4020

gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggaggat 4080

aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac 4140

ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc 4200

gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct 4260

cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg 4320

aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc 4380

gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag 4440

ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg 4500

gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag 4560

aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc 4620

aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc 4680

cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc 4740

ggcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc ttcgatgatg 4800

gatcaagcca ggagcgcctt cagcaatctg ttcggcggag agcctctgag ctacaccaga 4860

ttcagcctgg ccaggcaagt ggatggcgat aacagccacg tggagatgaa gctggctgtg 4920

gacgaagaag agaacgccga caacaacacc aaggccaacg tgaccaagcc taagagatgc 4980

agcggcagca tctgctatgg aaccatcgcc gtgatcgtgt tcttcctgat cggcttcatg 5040

atcggctacc tgggctactg tggcggcggc ggcagcggcg gcggcggcag cttggctact 5100

acacttgaac gtattgagaa gaactttgtc attactgacc caagattgcc agataatccc 5160

attattattcg cgtccgatag tttcttgcag ttgacagaat atagccgtga agaaattttg 5220

ggaagaaact gcaggtttct acaaggtcct gaaactgatc gcgcgacagt gagaaaaatt 5280

agagatgcca tagataacca aacagaggtc actgttcagc tgattaatta tacaaagagt 5340

ggtaaaaagt tctggaacct ctttcacttg cagcctatgc gagatcagaa gggagatgtc 5400

cagtacttta ttggggttca gttggatgga actgagcatg tccgagatgc tgccgagaga 5460

gagggagtca tgctgattaa gaaaactgca gaaaatattg atgaggcg 5508

<210> 10

<211> 5505

<212> DNA

<213> Artificial Sequence

<400> 10

cagtttacag atgtggattg cagcgtgagc aaggagtgct ggagcgtgtg caaggatctg 60

tttggagtgg atagaggaaa gtgcatgggc aagaagtgta gatgctacca gtagtctaga 120

taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc 180

ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 240

attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 300

tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta 360

aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 420

ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 480

agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 540

cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 600

atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 660

ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 720

gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 780

acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt 840

gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 900

acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag 960

aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 1020

agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 1080

tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 1140

cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 1200

ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 1260

cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac 1320

aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 1380

ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 1440

cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 1500

cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 1560

cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 1620

gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 1680

catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 1740

ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 1800

tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 1860

caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 1920

gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 1980

ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 2040

cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 2100

cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 2160

gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 2220

aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 2280

ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca 2340

ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt 2400

gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca 2460

ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca 2520

agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc 2580

tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 2640

taggtgaaga tccttttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 2700

cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc ttttttttctg 2760

cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 2820

gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 2880

aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 2940

cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 3000

tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 3060

acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 3120

ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 3180

ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 3240

tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 3300

tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 3360

ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 3420

gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt 3480

tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 3540

accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 3600

aatagggact ttccattgac gtcaatgggt ggattatta cggtaaactg cccacttggc 3660

agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 3720

gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 3780

ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg 3840

tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag 3900

tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt 3960

gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt 4020

gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggaggat 4080

aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac 4140

ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc 4200

gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct 4260

cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg 4320

aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc 4380

gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag 4440

ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg 4500

gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag 4560

aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc 4620

aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc 4680

cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc 4740

ggcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc ttcgatgatg 4800

gatcaagcca ggagcgcctt cagcaatctg ttcggcggag agcctctgag ctacaccaga 4860

ttcagcctgg ccaggcaagt ggatggcgat aacagccacg tggagatgaa gctggctgtg 4920

gacgaagaag agaacgccga caacaacacc aaggccaacg tgaccaagcc taagagatgc 4980

agcggcagca tctgctatgg aaccatcgcc gtgatcgtgt tcttcctgat cggcttcatg 5040

atcggctacc tgggctactg tggcggcggc ggcagcggcg gcggcggcag cttggctact 5100

acacttgaac gtattgagaa gaactttgtc attactgacc caagattgcc agataatccc 5160

attattattcg cgtccgatag tttcttgcag ttgacagaat atagccgtga agaaattttg 5220

ggaagaaact gcaggtttct acaaggtcct gaaactgatc gcgcgacagt gagaaaaatt 5280

agagatgcca tagataacca aacagaggtc actgttcagc tgattaatta tacaaagagt 5340

ggtaaaaagt tctggaacct ctttcacttg cagcctatgc gagatcagaa gggagatgtc 5400

cagtacttta ttggggttca gttggatgga actgagcatg tccgagatgc tgccgagaga 5460

gagggagtca tgctgattaa gaaaactgca gaaaatattg atgag 5505

<210> 11

<211> 5502

<212> DNA

<213> Artificial Sequence

<400> 11

cagtttacag atgtggattg cagcgtgagc aaggagtgct ggagcgtgtg caaggatctg 60

tttggagtgg atagaggaaa gtgcatgggc aagaagtgta gatgctacca gtagtctaga 120

taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc 180

ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 240

attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 300

tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta 360

aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 420

ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 480

agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 540

cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 600

atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 660

ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 720

gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 780

acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt 840

gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 900

acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag 960

aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 1020

agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 1080

tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 1140

cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 1200

ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 1260

cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac 1320

aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 1380

ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 1440

cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 1500

cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 1560

cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 1620

gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 1680

catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 1740

ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 1800

tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 1860

caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 1920

gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 1980

ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 2040

cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 2100

cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 2160

gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 2220

aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 2280

ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca 2340

ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt 2400

gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca 2460

ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca 2520

agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc 2580

tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 2640

taggtgaaga tccttttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 2700

cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc ttttttttctg 2760

cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 2820

gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 2880

aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 2940

cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 3000

tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 3060

acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 3120

ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 3180

ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 3240

tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 3300

tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 3360

ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 3420

gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt 3480

tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 3540

accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 3600

aatagggact ttccattgac gtcaatgggt ggattatta cggtaaactg cccacttggc 3660

agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 3720

gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 3780

ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg 3840

tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag 3900

tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt 3960

gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt 4020

gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggaggat 4080

aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac 4140

ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc 4200

gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct 4260

cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg 4320

aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc 4380

gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag 4440

ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg 4500

gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag 4560

aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc 4620

aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc 4680

cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc 4740

ggcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc ttcgatgatg 4800

gatcaagcca ggagcgcctt cagcaatctg ttcggcggag agcctctgag ctacaccaga 4860

ttcagcctgg ccaggcaagt ggatggcgat aacagccacg tggagatgaa gctggctgtg 4920

gacgaagaag agaacgccga caacaacacc aaggccaacg tgaccaagcc taagagatgc 4980

agcggcagca tctgctatgg aaccatcgcc gtgatcgtgt tcttcctgat cggcttcatg 5040

atcggctacc tgggctactg tggcggcggc ggcagcggcg gcggcggcag cttggctact 5100

acacttgaac gtattgagaa gaactttgtc attactgacc caagattgcc agataatccc 5160

attattattcg cgtccgatag tttcttgcag ttgacagaat atagccgtga agaaattttg 5220

ggaagaaact gcaggtttct acaaggtcct gaaactgatc gcgcgacagt gagaaaaatt 5280

agagatgcca tagataacca aacagaggtc actgttcagc tgattaatta tacaaagagt 5340

ggtaaaaagt tctggaacct ctttcacttg cagcctatgc gagatcagaa gggagatgtc 5400

cagtacttta ttggggttca gttggatgga actgagcatg tccgagatgc tgccgagaga 5460

gagggagtca tgctgattaa gaaaactgca gaaaatattg at 5502

<210> 12

<211> 5499

<212> DNA

<213> Artificial Sequence

<400> 12

cagtttacag atgtggattg cagcgtgagc aaggagtgct ggagcgtgtg caaggatctg 60

tttggagtgg atagaggaaa gtgcatgggc aagaagtgta gatgctacca gtagtctaga 120

taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc 180

ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 240

attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 300

tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta 360

aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 420

ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 480

agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 540

cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 600

atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 660

ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 720

gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 780

acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt 840

gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 900

acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag 960

aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 1020

agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 1080

tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 1140

cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 1200

ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 1260

cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac 1320

aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 1380

ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 1440

cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 1500

cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 1560

cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 1620

gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 1680

catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 1740

ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 1800

tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 1860

caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 1920

gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 1980

ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 2040

cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 2100

cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 2160

gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 2220

aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 2280

ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca 2340

ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt 2400

gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca 2460

ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca 2520

agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc 2580

tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 2640

taggtgaaga tccttttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 2700

cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc ttttttttctg 2760

cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 2820

gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 2880

aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 2940

cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 3000

tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 3060

acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 3120

ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 3180

ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 3240

tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 3300

tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 3360

ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 3420

gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt 3480

tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 3540

accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 3600

aatagggact ttccattgac gtcaatgggt ggattatta cggtaaactg cccacttggc 3660

agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 3720

gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 3780

ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg 3840

tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag 3900

tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt 3960

gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt 4020

gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggaggat 4080

aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac 4140

ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc 4200

gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct 4260

cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg 4320

aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc 4380

gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag 4440

ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg 4500

gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag 4560

aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc 4620

aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc 4680

cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc 4740

ggcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc ttcgatgatg 4800

gatcaagcca ggagcgcctt cagcaatctg ttcggcggag agcctctgag ctacaccaga 4860

ttcagcctgg ccaggcaagt ggatggcgat aacagccacg tggagatgaa gctggctgtg 4920

gacgaagaag agaacgccga caacaacacc aaggccaacg tgaccaagcc taagagatgc 4980

agcggcagca tctgctatgg aaccatcgcc gtgatcgtgt tcttcctgat cggcttcatg 5040

atcggctacc tgggctactg tggcggcggc ggcagcggcg gcggcggcag cttggctact 5100

acacttgaac gtattgagaa gaactttgtc attactgacc caagattgcc agataatccc 5160

attattattcg cgtccgatag tttcttgcag ttgacagaat atagccgtga agaaattttg 5220

ggaagaaact gcaggtttct acaaggtcct gaaactgatc gcgcgacagt gagaaaaatt 5280

agagatgcca tagataacca aacagaggtc actgttcagc tgattaatta tacaaagagt 5340

ggtaaaaagt tctggaacct ctttcacttg cagcctatgc gagatcagaa gggagatgtc 5400

cagtacttta ttggggttca gttggatgga actgagcatg tccgagatgc tgccgagaga 5460

gagggagtca tgctgattaa gaaaactgca gaaaatatt 5499

<210> 13

<211> 111

<212> DNA

<213> Artificial Sequence

<400> 13

cagtttacaa acgtcagctg cacaacaagc aaggagtgct ggagcgtgtg ccagagactg 60

cacaacacaa gcagaggaaa gtgcatgaac aagaagtgca gatgctacag c 111

<210> 14

<211> 195

<212> DNA

<213> Artificial Sequence

<400> 14

aagcttcagt ttacagatgt ggattgcagc gtgagcaagg agtgctggag cgtgtgcaag 60

gatctgtttg gagtggatag aggaaagtgc atgggcaaga agtgtagatg ctaccagggt 120

accgccgccg ccgactacaa ggacgacgac gacaagatcg acgccgccgc cggcggcgcc 180

ctgtgcaacg aattc 195

<210> 15

<211> 195

<212> DNA

<213> Artificial Sequence

<400> 15

aagcttcagt ttacaaacgt cagctgcaca acaagcaagg agtgctggag cgtgtgccag 60

agactgcaca acacaagcag aggaaagtgc atgaacaaga agtgcagatg ctacagcggt 120

accgccgccg ccgactacaa ggacgacgac gacaagatcg acgccgccgc cggcggcgcc 180

ctgtgcaacg aattc 195

<210> 16

<211> 72

<212> DNA

<213> Artificial Sequence

<400> 16

ggtaccgccg ccgccgacta caaggacgac gacgacaaga tcgacgccgc cgccggcggc 60

gccctgtgca ac 72

<210> 17

<211> 43

<212> DNA

<213> Artificial Sequence

<400> 17

ggacgacgac gacaagctga agcttggtac cgccgccgcc gac 43

<210> 18

<211> 28

<212> DNA

<213> Artificial Sequence

<400> 18

aagcttcagc ttgtcgtcgt cgtccttg 28

<210> 19

<211> 18

<212> DNA

<213> Artificial Sequence

<400> 19

gcggccgctc tagataac 18

<210> 20

<211> 20

<212> DNA

<213> Artificial Sequence

<400> 20

tgaattcgaa gcttgagctc 20

<210> 21

<211> 45

<212> DNA

<213> Artificial Sequence

<400> 21

atctcgagct caagcttcga attcaatgat ggatcaagcc aggag 45

<210> 22

<211> 48

<212> DNA

<213> Artificial Sequence

<400> 22

gctgccgccg ccgccgctgc cgccgccgcc acagtagccc aggtagcc 48

<210> 23

<211> 50

<212> DNA

<213> Artificial Sequence

<400> 23

ggcggcggcg gcagcggcgg cggcggcagc cagtttacag atgtggattg 50

<210> 24

<211> 44

<212> DNA

<213> Artificial Sequence

<400> 24

atgatcagtt atctagagcg gccgcctact ggtagcatct acac 44

<210> 25

<211> 44

<212> DNA

<213> Artificial Sequence

<400> 25

cagtttacag atgtggattg cagcgtgagc aaggagtgct ggag 44

<210> 26

<211> 18

<212> DNA

<213> Artificial Sequence

<400> 26

gctgccgccg ccgccgct 18

<210> 27

<211> 44

<212> DNA

<213> Artificial Sequence

<400> 27

cggcggcagc ggcggcggcg gcagcttggc tactacactt gaac 44

<210> 28

<211> 43

<212> DNA

<213> Artificial Sequence

<400> 28

cgctgcaatc cacatctgta aactgaagtt cttttgccgc ctc 43

<210> 29

<211> 43

<212> DNA

<213> Artificial Sequence

<400> 29

cgctgcaatc cacatctgta aactgttctt ttgccgcctc atc 43

<210> 30

<211> 49

<212> DNA

<213> Artificial Sequence

<400> 30

cgctgcaatc cacatctgta aactgttttg ccgcctcatc aatattttc 49

<210> 31

<211> 46

<212> DNA

<213> Artificial Sequence

<400> 31

cgctgcaatc cacatctgta aactgtgccg cctcatcaat attttc 46

<210> 32

<211> 45

<212> DNA

<213> Artificial Sequence

<400> 32

cgctgcaatc cacatctgta aactgcgcct catcaatatt ttctg 45

<210> 33

<211> 45

<212> DNA

<213> Artificial Sequence

<400> 33

cgctgcaatc cacatctgta aactgctcat caatattttc tgcag 45

<210> 34

<211> 47

<212> DNA

<213> Artificial Sequence

<400> 34

cgctgcaatc cacatctgta aactgatcaa tattttctgc agttttc 47

<210> 35

<211> 50

<212> DNA

<213> Artificial Sequence

<400> 35

cgctgcaatc cacatctgta aactgaatat tttctgcagt tttcttaatc 50

Claims (1)

1. A BK channel light-operated regulating element is characterized by comprising a peptide toxin, a light-sensitive protein LOV structural domain, a membrane localization protein and a fluorescent protein which are sequentially connected; the nucleotide sequence for coding the BK channel light-operated regulating element is shown as SEQ ID NO. 12.

Images