CN110804098B - BK channel light-operated adjusting element - Google Patents

BK channel light-operated adjusting element Download PDF

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CN110804098B
CN110804098B CN201911022935.9A CN201911022935A CN110804098B CN 110804098 B CN110804098 B CN 110804098B CN 201911022935 A CN201911022935 A CN 201911022935A CN 110804098 B CN110804098 B CN 110804098B
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李勃兴
黄潋滟
王裴
安芮昕
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Sun Yat Sen University
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Abstract

The invention provides a BK channel light-operated regulating element, which comprises a peptide toxin, a light-sensitive protein LOV structural domain, a membrane localization protein and a fluorescent protein. The BK channel light-operated adjusting element has high sensitivity, can realize the regulation and control of an endogenous BK channel, and realizes the precise optical regulation and control of the endogenous BK channel and the regulation and control of subcellular structures such as organelles in cells by utilizing the high time resolution and the high spatial resolution of light.

Description

BK channel light-operated adjusting element
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a BK channel light-operated adjusting element.
Background
Ion channels are the basis of life activities, and exist on cell membranes of animals or plants, unicellular organisms and multicellular organisms. The most basic function of ion channels is to generate cellular bioelectricity on excitatory nerve and muscle tissues, that is, to directly relate to the excitability of cells, and to derive important higher nervous activities such as release of neurotransmitters, secretion of glands, movement of muscles, and even learning and memory through the propagation of bioelectricity. Thus, ion channels are the basic excitatory units on excitable tissue membranes and have vital physiological functions. The research on the ion channel is directly connected with the research on the pathogenesis and the drug treatment of the ion channel related diseases.
A special type of the KCa channels with large conductance calcium ion activated potassium channels is the KCa channels (BK) with large conductance calcium ion activated potassium channels, the single-channel conductance of the BK can reach 100-300 ps, and the BK is obviously different from other family members, such as small conductance potassium channels (4-20 ps) and medium conductance potassium channels (20-80 ps). The BK channel is unique not only in its large single-channel conductance, but also in that it is exposed to intracellular Ca 2+ And the dual regulation of membrane depolarization signals, and the integration of the two signals, the BK channel becomes the coupled intracellular Ca 2+ In addition, signals such as cell membrane stress, cytokines, hormones, ATP, NO, etc. can also activate BK channels.
The main characteristic of optogenetic technology is the combination of optics and genetics, and the biological functions of neurons are controlled by light. Optogenetics was first discovered in the neural field and is now widely used in the cerebral neural circuits. The study on the regulation and control of nerve functions mainly adopts an implantable optical fiber catheter and three-dimensional directional transfer implantation, so that corresponding illumination acts on neurons, and the functions of rapid excitation, rapid inhibition, bistable modulation and intracellular biochemical information regulation and control of the neurons are realized.
As an essential tool in optogenetic technology, photoreceptors have been discovered or synthesized in many ways so far, and have been successfully used for studies on cellular functions, signal transduction, molecular functions at the sub-cellular level, and the like. Among them, light-oxygen-voltage sensing domain (LOV) domain protein is a photo receptor which is currently being researched more, has small relative molecular mass and solubility, and has many ways of regulating signal molecules because its required cofactors exist in most types of cells, which make LOV an ideal molecule for exploring photo-controlled ion channels. Previous studies have used various means to embed or attach different polypeptides and enzymes in proximity to the LOV domain (LOV regulation).
Ion channels are involved in regulating intracellular signaling and information integration in neurons and glial cells, and are also important drug targets for many neurological diseases. To assess the role of ion channels in the complex nervous system, gene-regulated ion channels are a desirable model. In the context of gene regulation of ion channels, there are several strategies: one aspect is the expression of genetically encoded membrane-targeted peptide toxins in different types of cells. The peptide toxin is composed of a series of polypeptides from toxic animal genetic code regulation ion channels, and can specifically recognize various ion channels. These toxins induce a reversible response over a period of hours to days without the addition of exogenous chemicals, and have been shown to be effective in the living brain of mammals. The second strategy is to use a photosensitive compound that anchors at specific positions in the ion channel (e.g., SPARK, LiGluR, HyLighter) and can induce a rapid and reversible light sensing response. They bind exogenous compounds and bind to the ion channel of interest. The first strategy is completely encoded by the gene, but cannot be regulated by photocontrol; the second strategy is through photomodulation, but the insertion of exogenous ion channels, and the inability to study the function of endogenous ion channels. There have been few studies on the regulation of endogenous ion channels by optogenetic means.
Disclosure of Invention
The invention aims to solve the technical problems and provides a BK channel light control adjusting element which is high in sensitivity and can effectively adjust and control a BK channel.
To this end, the invention provides a BK channel photocontrol modulator element, which comprises a peptide toxin, a light sensitive protein LOV structural domain, a membrane localization protein and a fluorescent protein.
Preferably, the membrane localization protein is a PDGF (platelet derived factor) receptor transmembrane protein or a transporter (transferrin) receptor transmembrane protein. Preferably, the fluorescent protein is a mCherry fluorescent protein.
The peptide toxin referred to herein is also called polypeptide toxin (polypeptide toxin), which is a kind of bioactive substance, and is an active polypeptide directly encoded by a gene. Preferably, the peptide toxin is an ebiximab scorpion toxin or a charybrid scorpion toxin.
Preferably, the BK channel light control adjusting element has, from N terminal to C terminal: a peptide toxin, a first linker (linker), a photoprotein LOV domain, a second linker, a transmembrane protein, and a mCherry fluorescent protein. The photoprotein LOV domain is linked to the second linker by its ja helix.
Preferably, the BK channel light control adjusting element has, from N terminal to C terminal: peptide toxin, light sensitive protein LOV structural domain, third joint, transmembrane protein, mCherry fluorescent protein. The photoprotein LOV domain is linked to the peptide toxin via its J α helix.
Preferably, the nucleotide sequences encoding the first, second and third linkers are as shown in SEQ ID No. 16.
Preferably, the BK channel is an endogenous BK channel.
Preferably, the ja helix fragment of the leptin LOV domain is truncated to a variant from amino acid 404 to 546, or from amino acid 404 to 545, or from amino acid 404 to amino acid 544, or from amino acid 404 to amino acid 543, or from amino acid 404 to amino acid 542, or from amino acid 404 to amino acid 541, or from amino acid 404 to amino acid 540, or from amino acid 404 to amino acid 539. Most preferably, the ja helix fragment of the photoprotein LOV domain is truncated to a variant from amino acid 404 to amino acid 539.
Experimental results show that the BK channel light-operated adjusting element is high in sensitivity, can realize adjustment and control of an endogenous BK channel, and realizes precise optical adjustment and control of the endogenous BK channel and adjustment and control of subcellular structures of organelles (such as a nuclear membrane, mitochondria and the like) and the like in a cell by utilizing high time resolution and high spatial resolution of light.
Drawings
Fig. 1 is a schematic view of the structural principle of the BK channel light control adjusting element of the present invention.
FIG. 2 is a schematic diagram of the electrophysiological results of the BK channel light control element of the present invention.
Fig. 3 is a schematic diagram of another structure of the BK channel light control adjusting element of the present invention.
FIG. 4 is the electrophysiological results of the BK channel photocontrol elements of the present invention modulating various variants of the BK channel.
Detailed Description
The technical solutions of the present invention will be further described below with reference to the accompanying drawings and specific examples, but the present invention is not limited to the following examples.
Unless otherwise specified, the reagents referred to below are all commercially available. For the sake of brevity, the parameters of the procedures, steps and equipment used are not described in detail in part of the procedures, it being understood that these are well known to those skilled in the art and are reproducible.
1 materials and methods
1.1 vectors, cells and strains
pcDNA3.1-DTXK-lumixin and pmCherry-C1-mTrim21 plasmids were purchased from Addgene, USA, respectively, official gazette numbers 51691 and 105516, respectively, competent DH5 α was purchased from Bioengineering (Shanghai) Inc., IBTX (Iberiotoxin, Eibotoxin), CHTX (Charybdotoxin, Calybrids scorpion toxin) nucleotide sequences are shown in SEQ ID NO.1, 13, and were synthesized by Bioengineering (Shanghai) Inc.
1.2 Main reagents and instruments
Fetal bovine serum, pancreatin cell digest, DMEM medium were purchased from Gibico, usa; lipofectamine 3000 was purchased from Invitrogen, USA; ampicillin and nucleic acid dyes were purchased from saiko bio ltd; 6 XDNA Loading Buffer and DNA marker are purchased from Takara Bio engineering (Dalian) Co., Ltd; restriction enzymes NdeI, XholI, Xba I, BamH I were purchased from NEB; high purity plasmid miniprep kit and endotoxin removal plasmid extraction kit were purchased from Omega.
1.3 construction of recombinant expression plasmids
1.3.1 construction of recombinant expression plasmids IBTX-lumixin, CHTX-lumitioxn and linker-lumitioxin (the nucleotide sequences of the plasmids are respectively shown as SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO. 4)
Human IBTX (Iberiotoxin), CHTX (Charybdotoxin) and linker sequence, wherein the linker nucleotide sequence is as follows: GGTACCGCCGCCGCCGACTACAAGGACGACGACGACAAGATCGACGCCGCCG CCGGCGGCGCCCTGTGCAAC (shown as SEQ ID NO. 16), and simultaneously connecting HindIII/EcorI enzyme cutting sites and carrying out gene synthesis, wherein the sizes of target gene fragments are all 195 bp. The synthesized IBTX + linker sequence (shown as SEQ ID NO. 14) and the synthesized CHTX + linker sequence (shown as SEQ ID NO. 15) are subjected to double enzyme digestion by HindIII and Ecori to respectively replace DTXK + linker fragments on pcDNA3.1-DTXK-lucitoxin, after nucleic acid electrophoresis purification and recovery, the E.coli DH5 alpha cells are transformed after being connected for 3 hours by using T4 DNA ligase of Invitrogen company, 8 clones are selected for bacterial liquid PCR verification, and plasmids are extracted after amplification culture of positive clones. IBTX-lumitoxin and CHTX-lumitioxn are obtained respectively. The recombinant expression plasmid is subjected to HindIII and EcorI double enzyme digestion identification respectively, and the positive plasmid is subjected to sequencing by the company of Biotechnology engineering (Shanghai) Ltd. Carrying out amplification culture on the recombinant bacteria with correct sequencing, extracting recombinant plasmids according to an endotoxin-free plasmid extraction kit of Omega company, and measuring the plasmid concentration by adopting a NanoDrop One ultramicro spectrophotometer.
Use of
Figure BDA0002247800990000041
Removing the IBTX gene sequence in the IBTX-lumixin by using the HiFi DNA Assembly seamless cloning kit to construct a linker-lumixin recombinant plasmid, wherein the primer sequence is as follows: forward primer 5 '-3': ggacgacgacgacaagctgaagcttGGTACCGCCGCCGCCGAC (SEQ ID NO.17), reverse primer 5 '-3': AAGCTTCAGCTTGTCGTCGTCGTCCTTG (SEQ ID NO. 18). In the same way, after PCR, double enzyme digestion verification and sequencing, the newly constructed linker-lucitoxin plasmid is subjected to amplification culture of positive clones and recombinant plasmid is extracted by an endotoxin-free extraction kit.
1.3.2IBTX optically-controlled regulation of BK channel plasmid construction-Optogenetic control of ion channel blocking (OCIB) (the nucleotide sequences of the plasmids are respectively shown in SEQ ID NO. 5-12)
The pmCherry-C1-mTrim21 is used as a vector plasmid, a primer is designed by utilizing a seamless cloning principle, a sequence behind an open reading frame mCherry is removed, and a transferrin receptor transmembrane segment and an IBTX sequence are inserted at the same time.
The primer sequences are as follows:
vector forward primer 5 '-3': GCGGCCGCTCTAGATAAC (SEQ ID NO.19),
vector reverse primer 5 '-3': TGAATTCGAAGCTTGAGCTC (SEQ ID NO.20),
transferrinR insert forward primer: atctcgagctcaagcttcgaattcaATGATGGATCAAGCCAGGAG (SEQ ID NO.21),
transferrinR insert reverse primer:
gctgccgccgccgccgctgccgccgccgccACAGTAGCCCAGGTAGCC(SEQ ID NO.22)，
IBTX insert forward primer:
ggcggcggcggcagcggcggcggcggcagcCAGTTTACAGATGTGGATTG(SEQ ID NO.23)，
IBTX insert reverse primer 5 '-3': atgatcagttatctagagcggccgcCTACTGGTAGCATCTACAC (SEQ ID NO. 24).
PCR amplification was performed using KOD DNA polymerase from TOYOBO, Inc., and the PCR product was subjected to agarose electrophoresisElectrophoresis, Omega kit gel cutting recovery and use
Figure BDA0002247800990000051
The HiFi DNA Assembly seamless cloning kit simultaneously ligates the vector with the transferrin receptor (transferrinR) and IBTX insert. Transforming E.coli DH5 alpha cells with the ligation products, selecting 8 clones for bacterial liquid PCR verification, sequencing positive clones by the company of Biotechnology engineering (Shanghai) GmbH, cloning correctly for amplification culture, and extracting plasmids.
Subsequently, LOV fragments of different lengths (from amino acid 404 to amino acid 546 to amino acid 404 to amino acid 539) were inserted into the above-described vector with the following primer sequences:
vector forward primer: CAGTTTACAGATGTGGATTGCAGCGTGAGCAAGGAGTGCTGGAG (SEQ ID NO.25),
vector reverse primer: GCTGCCGCCGCCGCCGCT (SEQ ID NO.26),
LOV fragment universal forward primer: cggcggcagcggcggcggcggcagcTTGGCTACTACACTTGAAC (SEQ ID NO.27),
LOV 546 fragment reverse primer: cgctgcaatccacatctgtaaactgAAGTTCTTTTGCCGCCTC (SEQ ID NO.28),
LOV 545 fragment reverse primer: cgctgcaatccacatctgtaaactgTTCTTTTGCCGCCTCATC (SEQ ID NO.29),
LOV 544 fragment reverse primer: cgctgcaatccacatctgtaaactgTTTTGCCGCCTCATCAATATTTTC (SEQ ID NO.30),
LOV 543 fragment reverse primer: cgctgcaatccacatctgtaaactgTGCCGCCTCATCAATATTTTC (SEQ ID NO.31),
LOV 542 fragment reverse primer: cgctgcaatccacatctgtaaactgCGCCTCATCAATATTTTCTG (SEQ ID NO.32),
LOV 541 fragment reverse primer: cgctgcaatccacatctgtaaactgCTCATCAATATTTTCTGCAG (SEQ ID NO.33),
LOV 540 fragment reverse primer: cgctgcaatccacatctgtaaactgATCAATATTTTCTGCAGTTTTC (SEQ ID NO.34),
LOV 539 fragment reverse primer: cgctgcaatccacatctgtaaactgAATATTTTCTGCAGTTTTCTTAATC (SEQ ID NO. 35).
PCR amplification with KOD DNA polymerase from TOYOBO, agarose electrophoresis of PCR product, gel cutting and recovery with Omega kit, and use
Figure BDA0002247800990000061
The HiFi DNA Assembly seamless cloning kit was ligated to the vector and LOV inserts of different lengths, respectively. The nucleotide sequences of the obtained product plasmids are respectively shown as SEQ ID NO.5 (corresponding to LOV404-546), SEQ ID NO.6 (corresponding to LOV 404-545), SEQ ID NO.7 (corresponding to LOV 404-544), SEQ ID NO.8 (corresponding to LOV 404-543), SEQ ID NO.9 (corresponding to LOV 404-542), SEQ ID NO.10 (corresponding to LOV 404-541), SEQ ID NO.11 (corresponding to LOV 404-540) and SEQ ID NO.12 (corresponding to LOV 404-539). Transforming E.coli DH5 alpha cells with the ligation products, randomly selecting 8 clones to perform bacterial liquid PCR verification, sequencing positive clones by the company of Biotechnology engineering (Shanghai) GmbH, cloning correctly for sequencing, performing amplification culture, and extracting plasmids.
1.4 transient transfection expression
The recombinant plasmids were transfected into HEK293T cells with the aid of a lip3000 from Invitrogen, respectively, and BK channel plasmids were co-transfected therewith. lip3000 lipofection step: HEK293T cells were seeded into 24-well plates and transfected when grown to 80% adherent.
Tube A: 25 μ L of Opti-MEM TM Dilution of 1. mu.L Lipofectamine in culture Medium TM 3000 reagent, mix well.
And (B) tube: to 0.5. mu.g of DNA was added 25. mu.L of Opti-MEM TM Adding 1 mu L P3000 of culture medium TM And (5) fully and uniformly mixing the reagents. Mix tube A and tube B at a ratio of 1:1, incubate at room temperature for 15 min. 50 μ L of DNA-lipid complex was added to each well of cells. The culture medium is returned to the 37 ℃ incubator and subjected to electrophysiological experiments after 24 hours.
1.5 patch clamp experiment steps:
1.5.1 preparation of microelectrodes: drawing the electrode to a length of about 10mm and a diameter of less than 0.2mm, and pulling the electrode
1.5.2 the procedure was set to perform a second draw to a final tip use diameter of 1-2 μm. The front end of the electrode is coated with silicone resin to reduce the capacitance between the electrode and the perfusion liquid. Pouring electrode liquid into the electrode: the tip of the electrode was first immersed in the internal solution for about 5 seconds and then inserted from the rear end of the electrode to the vicinity of the tip using a polypropylene syringe, the solution was filled to 1/4 length, and the air bubbles at the tip were flicked off.
1.5.3 the electrode is contacted with the cell membrane, and the microelectrode and the cell membrane are slightly sucked to form high-resistance sealing, the resistance reaches more than 1G omega, and then the cell membrane is broken by forcefully sucking to ensure that the liquid in the electrode is communicated with the liquid in the cell, and the potential of the whole cell can be recorded. When a high-resistance seal is formed, the electrode is slightly withdrawn, a small piece of cell membrane can be torn off, transmembrane ion current on the membrane is recorded, and if only one ion channel exists, single-channel current can be obtained.
When single-channel data is recorded, the clamped membrane potential is at-70 mV, after the current is stabilized, blue light with the wavelength of 450nm is irradiated for 20s, and the change of the current amplitude is recorded by using gapfree.
For whole cell data, the membrane potential was clamped at-70 mV, followed by depolarization to +40mV with a depolarization time of 300ms with a 450nm wavelength of blue light for 20 s.
2. Results
2.1IBTX light-operated Regulation of electrophysiological results of BK channel plasmids
FIG. 1 is a schematic diagram of the structure principle of IBTX light-controlled regulation of BK channel plasmid OCIB. The plasmid comprises the following components from the N end to the C end in sequence: IBTX peptide toxin, linker, light sensation protein LOV structural domain and J alpha helix, linker, PDGF transmembrane segment, mCherry fluorescent protein. As shown, the BK ion channel is an endogenous BK channel.
In the dark state, IBTX peptide toxins specifically bind to BK channels, reducing K ion efflux. Under the irradiation of blue light of 450nm, the LOV structural domain is sensitized, the J alpha conformation is changed, IBTX peptide toxin is lifted, the BK channel pore passage cannot be blocked by IBTX, and the outflow of K ions is increased.
According to the structure, three optically-controlled BK channel plasmids are respectively prepared: negative controls are linker-lumixin, CHTX-lumixin and IBTX-lumixin. IBTX is ibotoxin (ibberiotoxin) and CHTX is charybdotoxin (charybdotoxin).
FIG. 2 shows electrophysiological results (n ═ 6) before and after transfection of HEK293T cells with CHTX-lumixin, IBTX-lumixin and the negative control linker-lumixin to blue light, and after blue light, the currents of IBTX-lumixin and CHTX-lumixin BK increased. After light is removed, the current decreases. Therefore, both the CHTX-lumixin and the IBTX-lumixin have the function of regulating BK channels.
FIG. 3 is a schematic diagram of the structural principle of another IBTX light-controlled BK channel plasmid OCIB. As shown in the figure, the sequence from the N end to the C end of the plasmid is as follows: IBTX peptidotoxin, J alpha helix and light sensitive protein LOV structural domain, linker, transferrin receptor transmembrane segment and mCherry fluorescent protein. Wherein the BK ion channel is an endogenous BK channel.
In the dark state, IBTX peptide toxins specifically bind to BK channels, reducing K ion efflux. Under the irradiation of blue light of 450nm, the LOV structural domain is sensitized, the J alpha conformation is changed, IBTX peptide toxin is lifted, the BK channel pore passage cannot be blocked by IBTX, and the outflow of K ions is increased.
According to the structure, three optically-controlled BK channel plasmids are respectively prepared: negative controls are linker-lumixin, CHTX-lumixin and IBTX-lumixin. IBTX is ibotoxin (ibberiotoxin) and CHTX is charybdotoxin (charybdotoxin). After the HEK293T cells were transfected with CHTX-lumixin, IBTX-lumixin and negative control linker-lumixin, the electrophysiological results (n is 6) before and after blue light were given, and after blue light was given, the currents of IBTX-lumixin and CHTX-lumixin BK were increased. After light is removed, the current is reduced. Therefore, both the CHTX-lumixin and the IBTX-lumixin have the function of regulating BK channels.
In order to find the variant which can optimally regulate BK current, the LOV-J alpha is truncated into different variants, the variants are truncated from amino acid 404 to amino acid 546 to amino acid 404 to 539, after 293T blue light irradiation is transfected, the variants 539 (namely the fragment is from amino acid 404 to amino acid 539), 540 (namely the fragment is from amino acid 404 to amino acid 540), 541 (namely the fragment is from amino acid 404 to amino acid 541), 545 (namely the fragment is from amino acid 404 to amino acid 545) and 546 (namely the fragment is from amino acid 404 to amino acid 546) all increase BK current, wherein the 539 increases BK current at most (n is 5). As shown in fig. 4, is the electrophysiological result of IBTX modulating individual variants of the BK channel.
Sequence listing
<110> Zhongshan university
<120> BK channel light control adjusting element
<160> 35
<170> SIPOSequenceListing 1.0
<210> 1
<211> 111
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 1
cagtttacag atgtggattg cagcgtgagc aaggagtgct ggagcgtgtg caaggatctg 60
tttggagtgg atagaggaaa gtgcatgggc aagaagtgta gatgctacca g 111
<210> 2
<211> 6925
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 2
gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60
ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120
cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180
ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240
gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300
tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360
cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420
attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480
atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540
atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600
tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660
actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720
aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780
gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840
ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900
atgagcgccc tgctgatcct ggccctggtg ggcgccgccg tggccgacta caaggacgac 960
gacgacaagc tgaagcttca gtttacagat gtggattgca gcgtgagcaa ggagtgctgg 1020
agcgtgtgca aggatctgtt tggagtggat agaggaaagt gcatgggcaa gaagtgtaga 1080
tgctaccagg gtaccgccgc cgccgactac aaggacgacg acgacaagat cgacgccgcc 1140
gccggcggcg ccctgtgcaa cgaattcttg gctactacac ttgaacgtat tgagaagaac 1200
tttgtcatta ctgacccaag attgccagat aatcccatta tattcgcgtc cgatagtttc 1260
ttgcagttga cagaatatag ccgtgaagaa attttgggaa gaaactgcag gtttctacaa 1320
ggtcctgaaa ctgatcgcgc gacagtgaga aaaattagag atgccataga taaccaaaca 1380
gaggtcactg ttcagctgat taattataca aagagtggta aaaagttctg gaacctcttt 1440
cacttgcagc ctatgcgaga tcagaaggga gatgtccagt actttattgg ggttcagttg 1500
gatggaactg agcatgtccg agatgctgcc gagagagagg gagtcatgct gattaagaaa 1560
actgcagaaa atattgatga ggcggcaaaa gaacttagcg gccgcggtac tcgagttgct 1620
gtgggccagg acacgcagga ggtcatcgtg gtgccacact ccttgccctt taaggtggtg 1680
gtgatctcag ccatcctggc cctggtggtg ctcaccatca tctcccttat catcctcatc 1740
atgctttggc agaagaaacc acgtaggatt cgtatggtga gcaagggcga ggaggataac 1800
atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc 1860
cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc 1920
aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag 1980
ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag 2040
ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg 2100
gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg 2160
cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag 2220
gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg 2280
ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag 2340
aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac 2400
aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc 2460
ggcatggacg agctgtacaa gtaatctaga gggcccgttt aaacccgctg atcagcctcg 2520
actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc 2580
ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt 2640
ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat 2700
tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa 2760
agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg 2820
gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 2880
cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 2940
aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 3000
cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 3060
ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 3120
aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg 3180
ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc 3240
agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 3300
tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 3360
aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 3420
ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 3480
atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt 3540
ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg 3600
atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt 3660
ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct 3720
gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga 3780
ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg 3840
ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact 3900
ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg 3960
agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct 4020
gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg 4080
gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt 4140
tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg 4200
cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc 4260
ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag 4320
agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt 4380
cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt 4440
cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc 4500
cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca 4560
gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa 4620
tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 4680
ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac 4740
ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 4800
gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 4860
atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 4920
cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 4980
tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 5040
agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 5100
aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 5160
gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 5220
tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 5280
cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 5340
ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 5400
cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 5460
atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 5520
agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 5580
gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 5640
gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 5700
tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 5760
agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 5820
gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 5880
aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 5940
aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 6000
ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 6060
gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 6120
aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 6180
ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 6240
tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 6300
ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 6360
cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 6420
agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 6480
gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 6540
gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 6600
acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 6660
acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 6720
agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 6780
aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 6840
gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 6900
tccccgaaaa gtgccacctg acgtc 6925
<210> 3
<211> 6925
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 3
gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60
ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120
cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180
ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240
gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300
tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360
cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420
attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480
atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540
atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600
tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660
actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720
aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780
gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840
ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900
atgagcgccc tgctgatcct ggccctggtg ggcgccgccg tggccgacta caaggacgac 960
gacgacaagc tgaagcttca gtttacaaac gtcagctgca caacaagcaa ggagtgctgg 1020
agcgtgtgcc agagactgca caacacaagc agaggaaagt gcatgaacaa gaagtgcaga 1080
tgctacagcg gtaccgccgc cgccgactac aaggacgacg acgacaagat cgacgccgcc 1140
gccggcggcg ccctgtgcaa cgaattcttg gctactacac ttgaacgtat tgagaagaac 1200
tttgtcatta ctgacccaag attgccagat aatcccatta tattcgcgtc cgatagtttc 1260
ttgcagttga cagaatatag ccgtgaagaa attttgggaa gaaactgcag gtttctacaa 1320
ggtcctgaaa ctgatcgcgc gacagtgaga aaaattagag atgccataga taaccaaaca 1380
gaggtcactg ttcagctgat taattataca aagagtggta aaaagttctg gaacctcttt 1440
cacttgcagc ctatgcgaga tcagaaggga gatgtccagt actttattgg ggttcagttg 1500
gatggaactg agcatgtccg agatgctgcc gagagagagg gagtcatgct gattaagaaa 1560
actgcagaaa atattgatga ggcggcaaaa gaacttagcg gccgcggtac tcgagttgct 1620
gtgggccagg acacgcagga ggtcatcgtg gtgccacact ccttgccctt taaggtggtg 1680
gtgatctcag ccatcctggc cctggtggtg ctcaccatca tctcccttat catcctcatc 1740
atgctttggc agaagaaacc acgtaggatt cgtatggtga gcaagggcga ggaggataac 1800
atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc 1860
cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc 1920
aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag 1980
ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag 2040
ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg 2100
gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg 2160
cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag 2220
gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg 2280
ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag 2340
aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac 2400
aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc 2460
ggcatggacg agctgtacaa gtaatctaga gggcccgttt aaacccgctg atcagcctcg 2520
actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc 2580
ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt 2640
ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat 2700
tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa 2760
agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg 2820
gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 2880
cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 2940
aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 3000
cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 3060
ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 3120
aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg 3180
ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc 3240
agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 3300
tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 3360
aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 3420
ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 3480
atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt 3540
ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg 3600
atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt 3660
ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct 3720
gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga 3780
ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg 3840
ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact 3900
ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg 3960
agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct 4020
gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg 4080
gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt 4140
tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg 4200
cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc 4260
ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag 4320
agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt 4380
cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt 4440
cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc 4500
cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca 4560
gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa 4620
tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 4680
ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac 4740
ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 4800
gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 4860
atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 4920
cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 4980
tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 5040
agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 5100
aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 5160
gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 5220
tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 5280
cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 5340
ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 5400
cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 5460
atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 5520
agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 5580
gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 5640
gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 5700
tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 5760
agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 5820
gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 5880
aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 5940
aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 6000
ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 6060
gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 6120
aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 6180
ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 6240
tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 6300
ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 6360
cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 6420
agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 6480
gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 6540
gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 6600
acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 6660
acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 6720
agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 6780
aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 6840
gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 6900
tccccgaaaa gtgccacctg acgtc 6925
<210> 4
<211> 6814
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 4
gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60
ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120
cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180
ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240
gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300
tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360
cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420
attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480
atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540
atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600
tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660
actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720
aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780
gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840
ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900
atgagcgccc tgctgatcct ggccctggtg ggcgccgccg tggccgacta caaggacgac 960
gacgacaagc tgaagcttgg taccgccgcc gccgactaca aggacgacga cgacaagatc 1020
gacgccgccg ccggcggcgc cctgtgcaac gaattcttgg ctactacact tgaacgtatt 1080
gagaagaact ttgtcattac tgacccaaga ttgccagata atcccattat attcgcgtcc 1140
gatagtttct tgcagttgac agaatatagc cgtgaagaaa ttttgggaag aaactgcagg 1200
tttctacaag gtcctgaaac tgatcgcgcg acagtgagaa aaattagaga tgccatagat 1260
aaccaaacag aggtcactgt tcagctgatt aattatacaa agagtggtaa aaagttctgg 1320
aacctctttc acttgcagcc tatgcgagat cagaagggag atgtccagta ctttattggg 1380
gttcagttgg atggaactga gcatgtccga gatgctgccg agagagaggg agtcatgctg 1440
attaagaaaa ctgcagaaaa tattgatgag gcggcaaaag aacttagcgg ccgcggtact 1500
cgagttgctg tgggccagga cacgcaggag gtcatcgtgg tgccacactc cttgcccttt 1560
aaggtggtgg tgatctcagc catcctggcc ctggtggtgc tcaccatcat ctcccttatc 1620
atcctcatca tgctttggca gaagaaacca cgtaggattc gtatggtgag caagggcgag 1680
gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc 1740
gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc 1800
cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg 1860
tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac 1920
tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac 1980
ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag 2040
gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg 2100
ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc 2160
aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac 2220
aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc 2280
acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac 2340
tccaccggcg gcatggacga gctgtacaag taatctagag ggcccgttta aacccgctga 2400
tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct 2460
tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca 2520
tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag 2580
ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct 2640
gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca 2700
ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 2760
gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 2820
caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 2880
cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 2940
tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 3000
acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 3060
gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga 3120
atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 3180
gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 3240
gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 3300
ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 3360
tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag 3420
gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt 3480
tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc 3540
acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 3600
caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 3660
ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat 3720
cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 3780
gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 3840
ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 3900
cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 3960
tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 4020
ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc 4080
atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 4140
actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 4200
ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 4260
ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac 4320
tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc 4380
caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 4440
gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc 4500
agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 4560
ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat 4620
accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 4680
ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 4740
gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 4800
gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 4860
tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 4920
gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 4980
ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 5040
ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 5100
acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 5160
tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 5220
ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 5280
ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 5340
ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 5400
actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 5460
gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 5520
tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 5580
caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 5640
atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 5700
acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 5760
ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 5820
ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 5880
tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 5940
tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 6000
gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 6060
tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 6120
tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 6180
ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 6240
tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 6300
ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 6360
gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 6420
ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 6480
cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 6540
ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 6600
ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 6660
gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 6720
ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 6780
gcgcacattt ccccgaaaag tgccacctga cgtc 6814
<210> 5
<211> 5520
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 5
cagtttacag atgtggattg cagcgtgagc aaggagtgct ggagcgtgtg caaggatctg 60
tttggagtgg atagaggaaa gtgcatgggc aagaagtgta gatgctacca gtagtctaga 120
taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc 180
ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 240
attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 300
tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta 360
aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 420
ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 480
agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 540
cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 600
atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 660
ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 720
gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 780
acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt 840
gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 900
acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag 960
aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 1020
agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 1080
tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 1140
cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 1200
ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 1260
cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac 1320
aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 1380
ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 1440
cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 1500
cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 1560
cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 1620
gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 1680
catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 1740
ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 1800
tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 1860
caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 1920
gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 1980
ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 2040
cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 2100
cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 2160
gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 2220
aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 2280
ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca 2340
ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt 2400
gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca 2460
ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca 2520
agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc 2580
tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 2640
taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 2700
cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 2760
cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 2820
gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 2880
aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 2940
cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 3000
tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 3060
acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 3120
ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 3180
ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 3240
tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 3300
tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 3360
ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 3420
gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt 3480
tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 3540
accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 3600
aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc 3660
agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 3720
gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 3780
ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg 3840
tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag 3900
tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt 3960
gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt 4020
gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggaggat 4080
aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac 4140
ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc 4200
gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct 4260
cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg 4320
aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc 4380
gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag 4440
ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg 4500
gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag 4560
aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc 4620
aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc 4680
cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc 4740
ggcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc ttcgatgatg 4800
gatcaagcca ggagcgcctt cagcaatctg ttcggcggag agcctctgag ctacaccaga 4860
ttcagcctgg ccaggcaagt ggatggcgat aacagccacg tggagatgaa gctggctgtg 4920
gacgaagaag agaacgccga caacaacacc aaggccaacg tgaccaagcc taagagatgc 4980
agcggcagca tctgctatgg aaccatcgcc gtgatcgtgt tcttcctgat cggcttcatg 5040
atcggctacc tgggctactg tggcggcggc ggcagcggcg gcggcggcag cttggctact 5100
acacttgaac gtattgagaa gaactttgtc attactgacc caagattgcc agataatccc 5160
attatattcg cgtccgatag tttcttgcag ttgacagaat atagccgtga agaaattttg 5220
ggaagaaact gcaggtttct acaaggtcct gaaactgatc gcgcgacagt gagaaaaatt 5280
agagatgcca tagataacca aacagaggtc actgttcagc tgattaatta tacaaagagt 5340
ggtaaaaagt tctggaacct ctttcacttg cagcctatgc gagatcagaa gggagatgtc 5400
cagtacttta ttggggttca gttggatgga actgagcatg tccgagatgc tgccgagaga 5460
gagggagtca tgctgattaa gaaaactgca gaaaatattg atgaggcggc aaaagaactt 5520
<210> 6
<211> 5517
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 6
cagtttacag atgtggattg cagcgtgagc aaggagtgct ggagcgtgtg caaggatctg 60
tttggagtgg atagaggaaa gtgcatgggc aagaagtgta gatgctacca gtagtctaga 120
taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc 180
ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 240
attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 300
tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta 360
aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 420
ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 480
agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 540
cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 600
atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 660
ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 720
gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 780
acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt 840
gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 900
acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag 960
aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 1020
agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 1080
tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 1140
cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 1200
ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 1260
cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac 1320
aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 1380
ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 1440
cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 1500
cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 1560
cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 1620
gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 1680
catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 1740
ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 1800
tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 1860
caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 1920
gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 1980
ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 2040
cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 2100
cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 2160
gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 2220
aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 2280
ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca 2340
ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt 2400
gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca 2460
ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca 2520
agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc 2580
tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 2640
taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 2700
cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 2760
cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 2820
gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 2880
aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 2940
cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 3000
tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 3060
acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 3120
ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 3180
ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 3240
tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 3300
tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 3360
ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 3420
gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt 3480
tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 3540
accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 3600
aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc 3660
agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 3720
gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 3780
ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg 3840
tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag 3900
tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt 3960
gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt 4020
gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggaggat 4080
aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac 4140
ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc 4200
gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct 4260
cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg 4320
aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc 4380
gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag 4440
ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg 4500
gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag 4560
aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc 4620
aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc 4680
cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc 4740
ggcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc ttcgatgatg 4800
gatcaagcca ggagcgcctt cagcaatctg ttcggcggag agcctctgag ctacaccaga 4860
ttcagcctgg ccaggcaagt ggatggcgat aacagccacg tggagatgaa gctggctgtg 4920
gacgaagaag agaacgccga caacaacacc aaggccaacg tgaccaagcc taagagatgc 4980
agcggcagca tctgctatgg aaccatcgcc gtgatcgtgt tcttcctgat cggcttcatg 5040
atcggctacc tgggctactg tggcggcggc ggcagcggcg gcggcggcag cttggctact 5100
acacttgaac gtattgagaa gaactttgtc attactgacc caagattgcc agataatccc 5160
attatattcg cgtccgatag tttcttgcag ttgacagaat atagccgtga agaaattttg 5220
ggaagaaact gcaggtttct acaaggtcct gaaactgatc gcgcgacagt gagaaaaatt 5280
agagatgcca tagataacca aacagaggtc actgttcagc tgattaatta tacaaagagt 5340
ggtaaaaagt tctggaacct ctttcacttg cagcctatgc gagatcagaa gggagatgtc 5400
cagtacttta ttggggttca gttggatgga actgagcatg tccgagatgc tgccgagaga 5460
gagggagtca tgctgattaa gaaaactgca gaaaatattg atgaggcggc aaaagaa 5517
<210> 7
<211> 5514
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 7
cagtttacag atgtggattg cagcgtgagc aaggagtgct ggagcgtgtg caaggatctg 60
tttggagtgg atagaggaaa gtgcatgggc aagaagtgta gatgctacca gtagtctaga 120
taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc 180
ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 240
attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 300
tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta 360
aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 420
ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 480
agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 540
cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 600
atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 660
ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 720
gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 780
acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt 840
gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 900
acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag 960
aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 1020
agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 1080
tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 1140
cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 1200
ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 1260
cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac 1320
aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 1380
ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 1440
cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 1500
cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 1560
cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 1620
gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 1680
catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 1740
ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 1800
tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 1860
caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 1920
gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 1980
ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 2040
cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 2100
cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 2160
gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 2220
aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 2280
ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca 2340
ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt 2400
gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca 2460
ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca 2520
agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc 2580
tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 2640
taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 2700
cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 2760
cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 2820
gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 2880
aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 2940
cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 3000
tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 3060
acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 3120
ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 3180
ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 3240
tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 3300
tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 3360
ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 3420
gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt 3480
tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 3540
accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 3600
aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc 3660
agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 3720
gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 3780
ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg 3840
tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag 3900
tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt 3960
gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt 4020
gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggaggat 4080
aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac 4140
ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc 4200
gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct 4260
cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg 4320
aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc 4380
gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag 4440
ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg 4500
gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag 4560
aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc 4620
aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc 4680
cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc 4740
ggcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc ttcgatgatg 4800
gatcaagcca ggagcgcctt cagcaatctg ttcggcggag agcctctgag ctacaccaga 4860
ttcagcctgg ccaggcaagt ggatggcgat aacagccacg tggagatgaa gctggctgtg 4920
gacgaagaag agaacgccga caacaacacc aaggccaacg tgaccaagcc taagagatgc 4980
agcggcagca tctgctatgg aaccatcgcc gtgatcgtgt tcttcctgat cggcttcatg 5040
atcggctacc tgggctactg tggcggcggc ggcagcggcg gcggcggcag cttggctact 5100
acacttgaac gtattgagaa gaactttgtc attactgacc caagattgcc agataatccc 5160
attatattcg cgtccgatag tttcttgcag ttgacagaat atagccgtga agaaattttg 5220
ggaagaaact gcaggtttct acaaggtcct gaaactgatc gcgcgacagt gagaaaaatt 5280
agagatgcca tagataacca aacagaggtc actgttcagc tgattaatta tacaaagagt 5340
ggtaaaaagt tctggaacct ctttcacttg cagcctatgc gagatcagaa gggagatgtc 5400
cagtacttta ttggggttca gttggatgga actgagcatg tccgagatgc tgccgagaga 5460
gagggagtca tgctgattaa gaaaactgca gaaaatattg atgaggcggc aaaa 5514
<210> 8
<211> 5511
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 8
cagtttacag atgtggattg cagcgtgagc aaggagtgct ggagcgtgtg caaggatctg 60
tttggagtgg atagaggaaa gtgcatgggc aagaagtgta gatgctacca gtagtctaga 120
taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc 180
ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 240
attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 300
tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta 360
aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 420
ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 480
agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 540
cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 600
atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 660
ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 720
gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 780
acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt 840
gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 900
acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag 960
aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 1020
agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 1080
tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 1140
cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 1200
ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 1260
cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac 1320
aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 1380
ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 1440
cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 1500
cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 1560
cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 1620
gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 1680
catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 1740
ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 1800
tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 1860
caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 1920
gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 1980
ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 2040
cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 2100
cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 2160
gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 2220
aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 2280
ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca 2340
ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt 2400
gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca 2460
ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca 2520
agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc 2580
tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 2640
taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 2700
cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 2760
cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 2820
gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 2880
aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 2940
cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 3000
tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 3060
acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 3120
ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 3180
ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 3240
tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 3300
tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 3360
ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 3420
gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt 3480
tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 3540
accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 3600
aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc 3660
agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 3720
gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 3780
ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg 3840
tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag 3900
tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt 3960
gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt 4020
gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggaggat 4080
aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac 4140
ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc 4200
gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct 4260
cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg 4320
aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc 4380
gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag 4440
ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg 4500
gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag 4560
aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc 4620
aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc 4680
cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc 4740
ggcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc ttcgatgatg 4800
gatcaagcca ggagcgcctt cagcaatctg ttcggcggag agcctctgag ctacaccaga 4860
ttcagcctgg ccaggcaagt ggatggcgat aacagccacg tggagatgaa gctggctgtg 4920
gacgaagaag agaacgccga caacaacacc aaggccaacg tgaccaagcc taagagatgc 4980
agcggcagca tctgctatgg aaccatcgcc gtgatcgtgt tcttcctgat cggcttcatg 5040
atcggctacc tgggctactg tggcggcggc ggcagcggcg gcggcggcag cttggctact 5100
acacttgaac gtattgagaa gaactttgtc attactgacc caagattgcc agataatccc 5160
attatattcg cgtccgatag tttcttgcag ttgacagaat atagccgtga agaaattttg 5220
ggaagaaact gcaggtttct acaaggtcct gaaactgatc gcgcgacagt gagaaaaatt 5280
agagatgcca tagataacca aacagaggtc actgttcagc tgattaatta tacaaagagt 5340
ggtaaaaagt tctggaacct ctttcacttg cagcctatgc gagatcagaa gggagatgtc 5400
cagtacttta ttggggttca gttggatgga actgagcatg tccgagatgc tgccgagaga 5460
gagggagtca tgctgattaa gaaaactgca gaaaatattg atgaggcggc a 5511
<210> 9
<211> 5508
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 9
cagtttacag atgtggattg cagcgtgagc aaggagtgct ggagcgtgtg caaggatctg 60
tttggagtgg atagaggaaa gtgcatgggc aagaagtgta gatgctacca gtagtctaga 120
taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc 180
ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 240
attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 300
tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta 360
aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 420
ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 480
agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 540
cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 600
atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 660
ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 720
gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 780
acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt 840
gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 900
acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag 960
aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 1020
agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 1080
tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 1140
cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 1200
ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 1260
cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac 1320
aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 1380
ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 1440
cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 1500
cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 1560
cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 1620
gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 1680
catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 1740
ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 1800
tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 1860
caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 1920
gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 1980
ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 2040
cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 2100
cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 2160
gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 2220
aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 2280
ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca 2340
ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt 2400
gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca 2460
ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca 2520
agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc 2580
tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 2640
taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 2700
cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 2760
cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 2820
gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 2880
aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 2940
cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 3000
tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 3060
acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 3120
ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 3180
ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 3240
tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 3300
tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 3360
ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 3420
gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt 3480
tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 3540
accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 3600
aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc 3660
agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 3720
gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 3780
ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg 3840
tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag 3900
tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt 3960
gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt 4020
gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggaggat 4080
aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac 4140
ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc 4200
gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct 4260
cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg 4320
aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc 4380
gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag 4440
ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg 4500
gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag 4560
aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc 4620
aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc 4680
cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc 4740
ggcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc ttcgatgatg 4800
gatcaagcca ggagcgcctt cagcaatctg ttcggcggag agcctctgag ctacaccaga 4860
ttcagcctgg ccaggcaagt ggatggcgat aacagccacg tggagatgaa gctggctgtg 4920
gacgaagaag agaacgccga caacaacacc aaggccaacg tgaccaagcc taagagatgc 4980
agcggcagca tctgctatgg aaccatcgcc gtgatcgtgt tcttcctgat cggcttcatg 5040
atcggctacc tgggctactg tggcggcggc ggcagcggcg gcggcggcag cttggctact 5100
acacttgaac gtattgagaa gaactttgtc attactgacc caagattgcc agataatccc 5160
attatattcg cgtccgatag tttcttgcag ttgacagaat atagccgtga agaaattttg 5220
ggaagaaact gcaggtttct acaaggtcct gaaactgatc gcgcgacagt gagaaaaatt 5280
agagatgcca tagataacca aacagaggtc actgttcagc tgattaatta tacaaagagt 5340
ggtaaaaagt tctggaacct ctttcacttg cagcctatgc gagatcagaa gggagatgtc 5400
cagtacttta ttggggttca gttggatgga actgagcatg tccgagatgc tgccgagaga 5460
gagggagtca tgctgattaa gaaaactgca gaaaatattg atgaggcg 5508
<210> 10
<211> 5505
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 10
cagtttacag atgtggattg cagcgtgagc aaggagtgct ggagcgtgtg caaggatctg 60
tttggagtgg atagaggaaa gtgcatgggc aagaagtgta gatgctacca gtagtctaga 120
taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc 180
ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 240
attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 300
tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta 360
aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 420
ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 480
agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 540
cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 600
atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 660
ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 720
gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 780
acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt 840
gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 900
acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag 960
aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 1020
agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 1080
tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 1140
cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 1200
ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 1260
cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac 1320
aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 1380
ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 1440
cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 1500
cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 1560
cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 1620
gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 1680
catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 1740
ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 1800
tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 1860
caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 1920
gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 1980
ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 2040
cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 2100
cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 2160
gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 2220
aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 2280
ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca 2340
ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt 2400
gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca 2460
ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca 2520
agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc 2580
tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 2640
taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 2700
cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 2760
cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 2820
gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 2880
aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 2940
cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 3000
tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 3060
acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 3120
ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 3180
ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 3240
tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 3300
tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 3360
ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 3420
gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt 3480
tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 3540
accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 3600
aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc 3660
agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 3720
gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 3780
ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg 3840
tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag 3900
tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt 3960
gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt 4020
gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggaggat 4080
aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac 4140
ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc 4200
gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct 4260
cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg 4320
aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc 4380
gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag 4440
ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg 4500
gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag 4560
aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc 4620
aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc 4680
cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc 4740
ggcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc ttcgatgatg 4800
gatcaagcca ggagcgcctt cagcaatctg ttcggcggag agcctctgag ctacaccaga 4860
ttcagcctgg ccaggcaagt ggatggcgat aacagccacg tggagatgaa gctggctgtg 4920
gacgaagaag agaacgccga caacaacacc aaggccaacg tgaccaagcc taagagatgc 4980
agcggcagca tctgctatgg aaccatcgcc gtgatcgtgt tcttcctgat cggcttcatg 5040
atcggctacc tgggctactg tggcggcggc ggcagcggcg gcggcggcag cttggctact 5100
acacttgaac gtattgagaa gaactttgtc attactgacc caagattgcc agataatccc 5160
attatattcg cgtccgatag tttcttgcag ttgacagaat atagccgtga agaaattttg 5220
ggaagaaact gcaggtttct acaaggtcct gaaactgatc gcgcgacagt gagaaaaatt 5280
agagatgcca tagataacca aacagaggtc actgttcagc tgattaatta tacaaagagt 5340
ggtaaaaagt tctggaacct ctttcacttg cagcctatgc gagatcagaa gggagatgtc 5400
cagtacttta ttggggttca gttggatgga actgagcatg tccgagatgc tgccgagaga 5460
gagggagtca tgctgattaa gaaaactgca gaaaatattg atgag 5505
<210> 11
<211> 5502
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 11
cagtttacag atgtggattg cagcgtgagc aaggagtgct ggagcgtgtg caaggatctg 60
tttggagtgg atagaggaaa gtgcatgggc aagaagtgta gatgctacca gtagtctaga 120
taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc 180
ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 240
attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 300
tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta 360
aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 420
ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 480
agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 540
cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 600
atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 660
ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 720
gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 780
acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt 840
gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 900
acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag 960
aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 1020
agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 1080
tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 1140
cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 1200
ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 1260
cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac 1320
aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 1380
ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 1440
cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 1500
cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 1560
cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 1620
gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 1680
catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 1740
ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 1800
tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 1860
caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 1920
gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 1980
ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 2040
cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 2100
cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 2160
gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 2220
aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 2280
ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca 2340
ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt 2400
gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca 2460
ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca 2520
agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc 2580
tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 2640
taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 2700
cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 2760
cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 2820
gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 2880
aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 2940
cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 3000
tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 3060
acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 3120
ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 3180
ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 3240
tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 3300
tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 3360
ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 3420
gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt 3480
tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 3540
accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 3600
aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc 3660
agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 3720
gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 3780
ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg 3840
tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag 3900
tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt 3960
gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt 4020
gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggaggat 4080
aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac 4140
ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc 4200
gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct 4260
cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg 4320
aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc 4380
gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag 4440
ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg 4500
gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag 4560
aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc 4620
aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc 4680
cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc 4740
ggcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc ttcgatgatg 4800
gatcaagcca ggagcgcctt cagcaatctg ttcggcggag agcctctgag ctacaccaga 4860
ttcagcctgg ccaggcaagt ggatggcgat aacagccacg tggagatgaa gctggctgtg 4920
gacgaagaag agaacgccga caacaacacc aaggccaacg tgaccaagcc taagagatgc 4980
agcggcagca tctgctatgg aaccatcgcc gtgatcgtgt tcttcctgat cggcttcatg 5040
atcggctacc tgggctactg tggcggcggc ggcagcggcg gcggcggcag cttggctact 5100
acacttgaac gtattgagaa gaactttgtc attactgacc caagattgcc agataatccc 5160
attatattcg cgtccgatag tttcttgcag ttgacagaat atagccgtga agaaattttg 5220
ggaagaaact gcaggtttct acaaggtcct gaaactgatc gcgcgacagt gagaaaaatt 5280
agagatgcca tagataacca aacagaggtc actgttcagc tgattaatta tacaaagagt 5340
ggtaaaaagt tctggaacct ctttcacttg cagcctatgc gagatcagaa gggagatgtc 5400
cagtacttta ttggggttca gttggatgga actgagcatg tccgagatgc tgccgagaga 5460
gagggagtca tgctgattaa gaaaactgca gaaaatattg at 5502
<210> 12
<211> 5499
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 12
cagtttacag atgtggattg cagcgtgagc aaggagtgct ggagcgtgtg caaggatctg 60
tttggagtgg atagaggaaa gtgcatgggc aagaagtgta gatgctacca gtagtctaga 120
taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc 180
ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 240
attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 300
tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta 360
aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 420
ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 480
agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 540
cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 600
atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 660
ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 720
gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 780
acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt 840
gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 900
acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag 960
aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 1020
agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 1080
tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 1140
cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 1200
ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 1260
cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac 1320
aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 1380
ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 1440
cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 1500
cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 1560
cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 1620
gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 1680
catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 1740
ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 1800
tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 1860
caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 1920
gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 1980
ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 2040
cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 2100
cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 2160
gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 2220
aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 2280
ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca 2340
ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt 2400
gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca 2460
ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca 2520
agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc 2580
tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 2640
taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 2700
cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 2760
cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 2820
gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 2880
aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 2940
cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 3000
tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 3060
acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 3120
ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 3180
ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 3240
tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 3300
tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 3360
ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 3420
gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt 3480
tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 3540
accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 3600
aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc 3660
agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 3720
gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 3780
ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg 3840
tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag 3900
tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt 3960
gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt 4020
gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggaggat 4080
aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac 4140
ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc 4200
gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct 4260
cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg 4320
aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc 4380
gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag 4440
ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg 4500
gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag 4560
aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc 4620
aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc 4680
cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc 4740
ggcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc ttcgatgatg 4800
gatcaagcca ggagcgcctt cagcaatctg ttcggcggag agcctctgag ctacaccaga 4860
ttcagcctgg ccaggcaagt ggatggcgat aacagccacg tggagatgaa gctggctgtg 4920
gacgaagaag agaacgccga caacaacacc aaggccaacg tgaccaagcc taagagatgc 4980
agcggcagca tctgctatgg aaccatcgcc gtgatcgtgt tcttcctgat cggcttcatg 5040
atcggctacc tgggctactg tggcggcggc ggcagcggcg gcggcggcag cttggctact 5100
acacttgaac gtattgagaa gaactttgtc attactgacc caagattgcc agataatccc 5160
attatattcg cgtccgatag tttcttgcag ttgacagaat atagccgtga agaaattttg 5220
ggaagaaact gcaggtttct acaaggtcct gaaactgatc gcgcgacagt gagaaaaatt 5280
agagatgcca tagataacca aacagaggtc actgttcagc tgattaatta tacaaagagt 5340
ggtaaaaagt tctggaacct ctttcacttg cagcctatgc gagatcagaa gggagatgtc 5400
cagtacttta ttggggttca gttggatgga actgagcatg tccgagatgc tgccgagaga 5460
gagggagtca tgctgattaa gaaaactgca gaaaatatt 5499
<210> 13
<211> 111
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 13
cagtttacaa acgtcagctg cacaacaagc aaggagtgct ggagcgtgtg ccagagactg 60
cacaacacaa gcagaggaaa gtgcatgaac aagaagtgca gatgctacag c 111
<210> 14
<211> 195
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 14
aagcttcagt ttacagatgt ggattgcagc gtgagcaagg agtgctggag cgtgtgcaag 60
gatctgtttg gagtggatag aggaaagtgc atgggcaaga agtgtagatg ctaccagggt 120
accgccgccg ccgactacaa ggacgacgac gacaagatcg acgccgccgc cggcggcgcc 180
ctgtgcaacg aattc 195
<210> 15
<211> 195
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 15
aagcttcagt ttacaaacgt cagctgcaca acaagcaagg agtgctggag cgtgtgccag 60
agactgcaca acacaagcag aggaaagtgc atgaacaaga agtgcagatg ctacagcggt 120
accgccgccg ccgactacaa ggacgacgac gacaagatcg acgccgccgc cggcggcgcc 180
ctgtgcaacg aattc 195
<210> 16
<211> 72
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 16
ggtaccgccg ccgccgacta caaggacgac gacgacaaga tcgacgccgc cgccggcggc 60
gccctgtgca ac 72
<210> 17
<211> 43
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 17
ggacgacgac gacaagctga agcttggtac cgccgccgcc gac 43
<210> 18
<211> 28
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 18
aagcttcagc ttgtcgtcgt cgtccttg 28
<210> 19
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 19
gcggccgctc tagataac 18
<210> 20
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 20
tgaattcgaa gcttgagctc 20
<210> 21
<211> 45
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 21
atctcgagct caagcttcga attcaatgat ggatcaagcc aggag 45
<210> 22
<211> 48
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 22
gctgccgccg ccgccgctgc cgccgccgcc acagtagccc aggtagcc 48
<210> 23
<211> 50
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 23
ggcggcggcg gcagcggcgg cggcggcagc cagtttacag atgtggattg 50
<210> 24
<211> 44
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 24
atgatcagtt atctagagcg gccgcctact ggtagcatct acac 44
<210> 25
<211> 44
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 25
cagtttacag atgtggattg cagcgtgagc aaggagtgct ggag 44
<210> 26
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 26
gctgccgccg ccgccgct 18
<210> 27
<211> 44
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 27
cggcggcagc ggcggcggcg gcagcttggc tactacactt gaac 44
<210> 28
<211> 43
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 28
cgctgcaatc cacatctgta aactgaagtt cttttgccgc ctc 43
<210> 29
<211> 43
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 29
cgctgcaatc cacatctgta aactgttctt ttgccgcctc atc 43
<210> 30
<211> 49
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 30
cgctgcaatc cacatctgta aactgttttg ccgcctcatc aatattttc 49
<210> 31
<211> 46
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 31
cgctgcaatc cacatctgta aactgtgccg cctcatcaat attttc 46
<210> 32
<211> 45
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 32
cgctgcaatc cacatctgta aactgcgcct catcaatatt ttctg 45
<210> 33
<211> 45
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 33
cgctgcaatc cacatctgta aactgctcat caatattttc tgcag 45
<210> 34
<211> 47
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 34
cgctgcaatc cacatctgta aactgatcaa tattttctgc agttttc 47
<210> 35
<211> 50
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 35
cgctgcaatc cacatctgta aactgaatat tttctgcagt tttcttaatc 50

Claims (1)

1. A BK channel light-operated regulating element is characterized by comprising a peptide toxin, a light-sensitive protein LOV structural domain, a membrane localization protein and a fluorescent protein which are sequentially connected; the nucleotide sequence for coding the BK channel light-operated regulating element is shown as SEQ ID NO. 12.
CN201911022935.9A 2019-10-25 2019-10-25 BK channel light-operated adjusting element Active CN110804098B (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102643852B (en) * 2011-02-28 2015-04-08 华东理工大学 Optical controllable gene expression system
US9163094B2 (en) * 2011-09-27 2015-10-20 Massachusetts Institute Of Technology Light-activated fusion proteins and uses therefor
WO2017053629A2 (en) * 2015-09-22 2017-03-30 William Marsh Rice University Light-controlled gene delivery with virus vectors through incorporation of optogenetic proteins and genetic insertion of non-conformationally constrained peptides

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