CN110800615A - Circular plant tissue culture box - Google Patents
Circular plant tissue culture box Download PDFInfo
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- CN110800615A CN110800615A CN201911282057.4A CN201911282057A CN110800615A CN 110800615 A CN110800615 A CN 110800615A CN 201911282057 A CN201911282057 A CN 201911282057A CN 110800615 A CN110800615 A CN 110800615A
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- base
- upper cover
- tissue culture
- plant tissue
- culture box
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
Abstract
The invention relates to the technical field of plant tissue culture, in particular to a round plant tissue culture box which comprises an upper cover and a base, wherein the upper cover and the base are rotatably connected, and a limiting device which is matched with each other, limits a rotating angle and aligns the position is arranged at the joint of the upper cover and the base. According to the invention, the limiting device is arranged at the joint of the upper cover and the base, the inner thread of the upper cover at the position corresponding to the limiting device is provided with the bulge, the thread at the position corresponding to the base is provided with the notch, the upper cover and the base can be clamped only by rotating less than 180 degrees at the indication position corresponding to the closing position, and the number of turns of the thread at the joint of the cover and the base is reduced.
Description
Technical Field
The invention relates to the technical field of plant tissue culture, in particular to a circular plant tissue culture box.
Background
The tissue culture technique is a technique in which living isolated organs (e.g., roots, stems, leaves, stem segments, protoplasts), tissues or cells are placed in a culture medium under artificially created aseptic conditions and placed in an appropriate environment for continuous culture to obtain cells, tissues or individuals. The technology is widely applied to agriculture and biological and medical research.
The prior art has the following problems that the circular tissue culture container is designed, the number of turns of the thread is too large, the step of screwing the upper cover is long in consumed time, the number of wide-mouth threads is large, the upper part and the lower part are not easy to align, the cover screwing difficulty is easy to cause, and the cover screwing is repeated for a longer time. In addition, the aseptic filter membrane is directly adhered to the position of the ventilating hole in the upper cover of the tissue culture container, so that the cover is prevented from being discarded due to falling off after sterilization. Finally, when the container inoculated with the material is placed on a culture rack for culture, the bottom of the container is in direct contact with the culture rack, condensed water is easily generated in the container, and plants can absorb light more uniformly and reduce pollution caused by the condensed water.
Disclosure of Invention
For this reason, need provide a circular plant group and cultivate box with earth, the device reduces the upper cover closure and opens the obstacle that the in-process met through the rational arrangement and the cooperation function of each structure, and the simple operation nature promotes greatly, improves work efficiency, avoids the upper cover that drops after the filter membrane sterilization and leads to scrapping, reduces the waste of material, practices thrift the cost, and is difficult for producing the comdenstion water in the container, and the plant can more evenly absorb light and reduce the pollution that the comdenstion water caused.
In order to achieve the purpose, the invention provides a circular plant tissue culture box which comprises an upper cover and a base, wherein the upper cover and the base are rotatably connected, and a limiting device which is matched with each other, limits a rotating angle and aligns the rotating angle is arranged at the joint of the upper cover and the base. Stop device's setting reduces the upper cover and closes and open the obstacle that the in-process meets, and the simple operation nature promotes greatly, improves work efficiency.
Furthermore, stop device is including setting up in at least one arch of upper cover inner wall edge and setting up in at least one breach at base outer wall edge, at least one arch and at least one breach shape looks adaptation. The cooperation of arch and breach makes the upper cover can fix on the base, and aims at the arch breach rotation can be more accurate guarantee upper cover and base be connected.
Furthermore, the upper cover and the base are rotatably connected through threads, the protrusions are arranged on internal threads on the edge of the inner wall of the upper cover, and the notches are arranged on external threads on the edge of the outer wall of the base. Threaded connection makes, upper cover and base connection convenient and fast more, and the cooperation setting of arch and breach for only need rotate less than 180 degrees and can fix when rotating at the bottom of the upper cover through the screw thread and connecting.
Furthermore, the width of the internal thread and the external thread is the relative displacement distance of the upper cover and the base which rotate for one circle, and the rotation angle of the bulge relative to the horizontal plane is less than 180 degrees. The thread rotating distance is shortened to one circle, so that the operation convenience is greatly improved, and the working efficiency is improved.
Furthermore, the number of the limiting devices is two, and the two limiting devices are oppositely arranged at the joint of the upper cover and the base. The number of the limiting devices is two, so that the rotating angles of the upper cover and the base are limited to a greater extent.
Furthermore, the number of the limiting devices is four, and the four limiting devices are uniformly distributed at the joint of the upper cover and the base for a circle. The number of the limiting devices is four, so that the rotation angles of the upper cover and the base are limited to a greater extent.
Furthermore, the top of the upper cover is provided with an air hole, and a filter membrane is embedded in the air hole of the upper cover. The arrangement of the air holes enables air to flow in the upper cover, and the filter membrane prevents external moisture and mixed bacteria from entering.
Furthermore, a plurality of positioning bulges are arranged at the bottom of the base. The base can be stabilized in the bellied setting of location, prevents the higher cultivation frame surface of base contact temperature simultaneously to arouse the inside condensation drop that appears of container, the drop of water condenses and can lead to the inside plant of container to receive the illumination inhomogeneous at upper cover internal surface.
Furthermore, the vertical section of the upper cover is a trapezoidal cross section with a small upper part and a large lower part, and the vertical section of the base is a trapezoidal cross section with a large upper part and a small lower part. The arrangement of the structure enables a large operation area to be formed between the upper cover and the base, and then the operation is carried out by personnel.
Furthermore, the cross sections of the upper cover and the base are circular cross sections. The circular cross-section arrangement is such that the upper cover and base facilitate a threaded connection.
Different from the prior art, the technical scheme has the following beneficial effects:
1. according to the invention, the limiting device is arranged at the joint of the upper cover and the base, the inner thread of the upper cover at the position corresponding to the limiting device is provided with the bulge, the thread at the position corresponding to the base is provided with the notch, the upper cover and the base can be clamped only by rotating less than 180 degrees at the indication position corresponding to the closing position, and the number of turns of the thread at the joint of the cover and the base is reduced.
2. According to the invention, the filter membrane is directly embedded on the air holes, so that the upper cover is prevented from being discarded due to the falling off of the filter membrane after sterilization.
3. The bottom of the container base is provided with the positioning bulge, so that the base is prevented from contacting the surface of the culture shelf with higher temperature, condensed water drops are generated in the container, and the condensation of the water drops on the inner surface of the upper cover can cause uneven illumination of plants in the container and easy plant pollution.
Drawings
FIG. 1 is a schematic structural diagram of a circular plant tissue culture box in the background art;
FIG. 2 is a sectional view of the plant tissue culture cassette according to the embodiment;
FIG. 3 is a schematic cross-sectional view of the upper cover according to the embodiment;
FIG. 4 is an enlarged schematic view of the top cover of the preferred embodiment at section A;
description of reference numerals:
1. the device comprises an upper cover, 101, air holes, 2, a base, 201, positioning protrusions, 3, a limiting device, 301, protrusions, 302 and notches.
Detailed Description
To explain technical contents, structural features, and objects and effects of the technical solutions in detail, the following detailed description is given with reference to the accompanying drawings in conjunction with the embodiments.
Referring to fig. 1-4, the circular plant tissue culture box of the present embodiment includes an upper cover 1 and a base 2, wherein a vertical section of the upper cover 1 is a trapezoid cross section with a small top and a large bottom, and a vertical section of the base 2 is a trapezoid cross section with a large top and a small bottom. The cross sections of the upper cover 1 and the base 2 are both circular cross sections. The center position of the top of the upper cover 1 is provided with a round air hole 101, and a filter membrane is embedded in the air hole 101 of the upper cover 1. The bottom of the base 2 is provided with a plurality of positioning protrusions 201. Upper cover 1 and base 2 swivelling joint, the lower edge of the inner wall of upper cover 1 is equipped with the internal thread, the last edge of base 2 outer wall is equipped with the external screw thread, upper cover 1 and base 2 rotate through the screw thread and connect, just upper cover 1 and base 2 junction are equipped with mutually supporting and prescribe a limit to the stop device 3 of rotation angle and alignment position. Specifically, the joint of the upper cover 1 and the base 2 is provided with a fixing ring, and the limiting device 3 is arranged on the fixing ring. The number of the positioning protrusions 201 is four, and the four positioning protrusions 201 are arranged around the bottom of the base 2 at equal intervals.
In this embodiment, the limiting device 3 includes at least one protrusion 3 disposed on the edge of the inner wall of the upper cover 1 and at least one notch 302 disposed on the edge of the outer wall of the base 2, and the protrusion 3 and the notch 302 are adapted in shape. The protrusion 3 is arranged on the internal thread at the edge of the inner wall of the upper cover 1, and the notch 302 is arranged on the external thread at the edge of the outer wall of the base 2. The width of the internal thread and the external thread is the relative displacement distance of the upper cover 1 and the base 2 which rotate for a circle, the rotation angle of the bulge 3 relative to the horizontal plane is less than 180 degrees, and the angle is the angle of the bulge 3 rotating around the central point of the base 2 when the upper cover 1 rotates. Specifically, 3 numbers of stop device are two, and two stop device 3 set up in upper cover 1 and base 2 junction relatively, and two stop device 3 symmetries set up, in other embodiments, 3 numbers of stop device are four, and four stop device 3 evenly distributed set up in upper cover 1 and base 2 junction a week, and four stop device 3 equidistance encircle upper cover 1 and base 2 setting. Wherein each set of stop means 3 comprises a protrusion 3 and a notch 302.
When specifically using, inlay its filter membrane on the bleeder vent 101 of upper cover 1 earlier, then take away high temperature autoclaving with upper cover 1 and base 2 that assemble, then place the plant that will prepare in base 2, then it is empty to aim at on base 2 stop device 3 with the last arch 3 of upper cover 1 stop device 3, then cover 1 at base 2, the external screw thread through upper cover 1 and base 2 rotates a week and is connected, thereby it is fixed in the breach 302 of base 2 to make the lug gomphosis on the upper cover 1, place whole box on the assigned position at last, fix through the protruding 201 fixed stay whole box of location on the base 2.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or terminal that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or terminal. Without further limitation, an element defined by the phrases "comprising … …" or "comprising … …" does not exclude the presence of additional elements in a process, method, article, or terminal that comprises the element. Further, herein, "greater than," "less than," "more than," and the like are understood to exclude the present numbers; the terms "above", "below", "within" and the like are to be understood as including the number.
Although the embodiments have been described, once the basic inventive concept is obtained, other variations and modifications of these embodiments can be made by those skilled in the art, so that the above embodiments are only examples of the present invention, and not intended to limit the scope of the present invention, and all equivalent structures or equivalent processes using the contents of the present specification and drawings, or any other related technical fields, which are directly or indirectly applied thereto, are included in the scope of the present invention.
Claims (10)
1. A circular plant tissue culture box is characterized in that: the rotary table comprises an upper cover and a base, wherein the upper cover is rotatably connected with the base, and a limiting device which is matched with the base and limits a rotating angle and aligns the position is arranged at the joint of the upper cover and the base.
2. The round plant tissue culture box according to claim 1, wherein: the limiting device comprises at least one protrusion arranged on the edge of the inner wall of the upper cover and at least one notch arranged on the edge of the outer wall of the base, and the protrusion is matched with the notch in shape.
3. The round plant tissue culture box according to claim 2, wherein: the upper cover and the base are rotatably connected through threads, the protrusions are arranged on internal threads on the edge of the inner wall of the upper cover, and the notches are arranged on external threads on the edge of the outer wall of the base.
4. The round plant tissue culture box according to claim 3, wherein: the width of the internal thread and the external thread is the relative displacement distance of the upper cover and the base which rotate for a circle, and the rotation angle of the bulge relative to the horizontal plane is less than 180 degrees.
5. The round plant tissue culture box according to claim 1, wherein: the number of the limiting devices is two, and the two limiting devices are oppositely arranged at the joint of the upper cover and the base.
6. The round plant tissue culture box according to claim 1, wherein: the number of the limiting devices is four, and the four limiting devices are uniformly distributed at the joint of the upper cover and the base for a circle.
7. The round plant tissue culture box according to claim 1, wherein: the top of the upper cover is provided with a vent hole, and a filter membrane is embedded in the vent hole. The filter membrane is integrated with the upper cover.
8. The round plant tissue culture box according to claim 1, wherein: the base bottom is equipped with a plurality of location archs.
9. The round plant tissue culture box according to any one of claims 1 to 8, wherein: the upper cover vertical section is a trapezoid cross section with a small upper part and a large lower part, and the base vertical section is a trapezoid cross section with a large upper part and a small lower part.
10. The round plant tissue culture cassette according to any one of claims 1 to 9, wherein: the cross sections of the upper cover and the base are circular cross sections.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911282057.4A CN110800615A (en) | 2019-12-13 | 2019-12-13 | Circular plant tissue culture box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911282057.4A CN110800615A (en) | 2019-12-13 | 2019-12-13 | Circular plant tissue culture box |
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| Publication Number | Publication Date |
|---|---|
| CN110800615A true CN110800615A (en) | 2020-02-18 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911282057.4A Pending CN110800615A (en) | 2019-12-13 | 2019-12-13 | Circular plant tissue culture box |
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Citations (7)
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|---|---|---|---|---|
| DE202013105106U1 (en) * | 2013-11-13 | 2013-11-21 | Wei-Ting Tsai | tool container |
| CN204742124U (en) * | 2015-05-26 | 2015-11-11 | 昆明理工大学 | Plant tissue culture bottle lid of adjustable throughput |
| CN106754359A (en) * | 2016-12-16 | 2017-05-31 | 湖州师范学院 | A kind of plant callus culture vessel |
| DE102016111875B3 (en) * | 2016-06-29 | 2017-12-07 | Beto Engineering and Marketing Co., Ltd. | Pump head with a lathe chuck |
| CN207143237U (en) * | 2017-08-09 | 2018-03-27 | 新乡医学院三全学院 | Preclinical medicine pathogen culture ware |
| CN108464239A (en) * | 2018-03-22 | 2018-08-31 | 福建省中科生物股份有限公司 | A kind of plant tissue culture container |
| CN209499368U (en) * | 2018-11-06 | 2019-10-18 | 武义新喜航工贸有限公司 | A kind of Novel cup |
-
2019
- 2019-12-13 CN CN201911282057.4A patent/CN110800615A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE202013105106U1 (en) * | 2013-11-13 | 2013-11-21 | Wei-Ting Tsai | tool container |
| CN204742124U (en) * | 2015-05-26 | 2015-11-11 | 昆明理工大学 | Plant tissue culture bottle lid of adjustable throughput |
| DE102016111875B3 (en) * | 2016-06-29 | 2017-12-07 | Beto Engineering and Marketing Co., Ltd. | Pump head with a lathe chuck |
| CN106754359A (en) * | 2016-12-16 | 2017-05-31 | 湖州师范学院 | A kind of plant callus culture vessel |
| CN207143237U (en) * | 2017-08-09 | 2018-03-27 | 新乡医学院三全学院 | Preclinical medicine pathogen culture ware |
| CN108464239A (en) * | 2018-03-22 | 2018-08-31 | 福建省中科生物股份有限公司 | A kind of plant tissue culture container |
| CN209499368U (en) * | 2018-11-06 | 2019-10-18 | 武义新喜航工贸有限公司 | A kind of Novel cup |
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