CN110794146A - Total protein quantitative detection dry tablet for in vitro diagnosis - Google Patents
Total protein quantitative detection dry tablet for in vitro diagnosis Download PDFInfo
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- CN110794146A CN110794146A CN201911055270.1A CN201911055270A CN110794146A CN 110794146 A CN110794146 A CN 110794146A CN 201911055270 A CN201911055270 A CN 201911055270A CN 110794146 A CN110794146 A CN 110794146A
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- vitro diagnosis
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
Abstract
The invention provides a total protein quantitative detection dry sheet for in vitro diagnosis, which comprises an upper shell with a sample dripping hole, a diffusion layer, a reagent layer, a resin layer, a support layer and a lower shell with a light hole, wherein the upper shell and the lower shell are mutually attached, the sample dripping hole is formed in the center of the upper shell, the light hole is formed in the center of the lower shell, the diffusion layer, the reagent layer, the resin layer and the support layer are sequentially arranged between the upper shell and the lower shell from top to bottom and are sequentially laminated and attached together from top to bottom through a coating process, the reagent layer is a region where a substance to be detected undergoes a chemical reaction, and the reagent layer comprises potassium sodium tartrate, copper salt, an alkaline compound and an alkaline resistant polymer. The invention solves the problems that the prior fluorescence photometry technology has higher requirements on instruments, a spectrophotometer which can be used for detection is expensive in a liquid phase, and the result can not be stored for a long time.
Description
Technical Field
The invention relates to an in vitro diagnosis detection reagent, in particular to a total protein quantitative detection dry sheet for in vitro diagnosis.
Background
So-called "dry-patch reagents" are relatively conventional "wet chemistry". The method is a mode that liquid in a detected sample is used as a reaction medium, and an object to be detected directly reacts with a reagent fixed on a carrier, and the method is different from the traditional wet chemistry in the medium participating in the chemical reaction. With the development of techniques for separation, purification, storage, etc. of enzymes in biochemistry, the progress of sensor, photometer, and electrode techniques, and the popularization of computers, dry chemistry has also rapidly developed.
Compared with wet chemistry, the dry chemistry analysis method has the advantages of corresponding detection instruments, simple operation, short detection time, accurate obtained result and the like. The structure of the reagent dry plate is from qualitative to semi-quantitative two-layer structure to multilayer film structure capable of accurately quantifying from the initial urine to the present whole blood, serum, plasma or other liquid, so that the application range of the dry chemical test paper method in clinical examination is obviously expanded, more and more biochemical examination projects can be carried out, and the inventor of Olson company in America advances
VITORS 350 is suitable for emergency treatment and conventional biochemical full-automatic analysis systems, can detect samples such as serum, plasma, whole blood, urine, cerebrospinal fluid and the like, and detection items comprise combined items such as liver function, kidney function, cardiac myozyme, blood fat, protein, ions and the like or any single item, so that the rapid and accurate diagnosis result is really realized, the clinical accurate, rapid and flexible diagnosis requirements can be fully met, but the matched instrument is too large, and the detection can not be realized at any time and in any place.
With the progress of biotechnology, the immune dry sheet containing enzyme markers and fluorescence markers, especially colloidal gold or selenium-labeled antibodies (or antigens) developed by utilizing technologies such as immunoosmosis, immunochromatography and the like can be used for analytical determination of troponin, special proteins, hormones, certain therapeutic drugs, virus antibodies or antigens and the like, the multilayer dry sheet adopting a fluorescence photometry and a matched instrument thereof are also available and are used more and more, and a vaginitis quintuplet detection reagent of Henan atlas biology company is used for detecting specific biochemical markers by a dry chemical enzyme method and is used for diagnosis of gynecological diseases. After more than 20 years of development, dry chemistry analysis techniques have been widely used to examine various aspects of medicine, including routine biochemistry, endocrine hormone, toxin drug concentration analysis, and special protein immunoassays. However, the requirement of the fluorescence photometry technology on instruments is high, a spectrophotometer capable of being used for detection is expensive in a liquid phase, and the result cannot be stored for a long time; the colloidal gold technology is used as a primary screening diagnostic reagent for the auxiliary treatment of disease diagnosis because the quality of a product is greatly different, the quality cannot be controlled, the quality cannot be ensured, and the phenomenon of the back zone exists, and a specimen needs to be diluted and tested, can only be qualitative and cannot be quantitative.
Disclosure of Invention
The invention provides a total protein quantitative detection dry sheet for in vitro diagnosis, which aims to solve the problems that the prior art has higher requirements on instruments by a fluorescence photometry technology, a spectrophotometer capable of being used for detection is expensive in a liquid phase, and the result cannot be stored for a long time; and the colloidal gold technology has the problems that the product quality is relatively large in difference, the quality cannot be controlled, the quality cannot be ensured, the phenomenon of back zone exists, a sample needs to be diluted and tested, and only qualitative and quantitative measurement can be carried out.
In order to solve the technical problems, the invention provides a total protein quantitative determination dry sheet for in vitro diagnosis, which comprises an upper shell with a sample dripping hole, a diffusion layer, a reagent layer, a resin layer, a support layer and a lower shell with a light hole, wherein the upper shell and the lower shell are mutually attached, the sample dripping hole is formed in the center of the upper shell, the light hole is formed in the center of the lower shell, the diffusion layer, the reagent layer, the resin layer and the support layer are sequentially arranged between the upper shell and the lower shell from top to bottom and are sequentially laminated and attached together from top to bottom through a coating process, the reagent layer is a region where a substance to be detected undergoes a chemical reaction, and the reagent layer comprises potassium sodium tartrate, copper salt, an alkaline compound and an alkaline resistant polymer.
The diffusion layer mainly comprises a hydrophilic high polymer material and a white reflecting material, the hydrophilic high polymer material is cellulose acetate or carboxymethyl cellulose, the white reflecting material is one or a mixture of more than one reflecting material of titanium dioxide, barium sulfate, polystyrene and microcrystalline cellulose, the wet film thickness of the diffusion layer is 300-500 mu m, and the dry film thickness is 70-300 mu m.
The potassium sodium tartrate is tartrate, and the tartrate comprises sodium tartrate, potassium tartrate, ammonium tartrate or lithium tartrate; the copper salt is aqueous copper salt, including copper sulfate, copper nickel triacylate, copper chloride, copper bromide, copper iodide or copper acetate; the pH value of the alkaline compound is more than 12.0, and the alkaline compound comprises sodium hydroxide, potassium hydroxide, lithium hydroxide or calcium hydroxide; the alkali-resistant polymer comprises agarose, starch, polyvinyl pyrrolidone, polyvinyl pyridine, methyl cellulose, methyl hydroxypropyl cellulose or polyvinyl alcohol.
The wet film thickness of the reagent layer is 100-300 mu m, and the dry film thickness is 40-200 mu m.
The material of the supporting layer can be composed of various transparent plastics, the thickness of the supporting layer is 30-200 mu m, the light transmittance of the supporting layer in a visible light range is more than 85%, and both an incident light source and reflected light can be ensured to pass through the supporting layer.
The transparent plastic is made of Polyethylene (PE), polypropylene (PP), Polyester Methyl Methacrylate (PMMA), Polystyrene (PS), Polycarbonate (PC) or polyethylene terephthalate (PET).
The resin layer is arranged between the supporting layer and the reagent layer coating film, the supporting layer and the reagent layer are adhered together by the resin layer with adhesion, and the resin layer is a novel resin system taking water instead of an organic solvent as a dispersion medium.
The material of the resin layer is one of epoxy resin, alkyd resin, polyester resin, polyurethane resin and modified polybutadiene resin, the wet film thickness of the resin layer is 10-100 mu m, and the dry film thickness is 2-50 mu m.
The upper shell and the lower shell are mainly made of plastic materials, and the upper shell layer and the lower shell layer are attached together in an ultrasonic attaching mode.
The invention has the following beneficial effects: the total protein quantitative detection dry tablet for in vitro diagnosis provided by the invention is a dry chemical method multi-layer membrane reagent dry tablet for in vitro diagnosis, has the advantages of rapid diagnosis, simple operation, cost saving and the like, can improve the accuracy, precision and sensitivity of detection, and has traceability, stable reagent and long storage time.
Drawings
FIG. 1 is a schematic structural diagram of a quantitative total protein detection dry plate for in vitro diagnosis according to an embodiment of the present invention.
Wherein, 1-upper shell, 2-diffusion layer, 3-reagent layer, 4-resin layer, 5-support layer, 6-lower shell, 7-sample dropping hole, and 8-light hole.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail below with reference to the accompanying drawings and specific embodiments.
As shown in figure 1, the invention provides a total protein quantitative determination dry plate for in vitro diagnosis, which comprises an upper shell 1 with a sample dripping hole, a diffusion layer 2, a reagent layer 3, a resin layer 4, a support layer 5 and a lower shell 6 with a light hole, wherein the upper shell 1 and the lower shell 6 are mutually attached, the central position of the upper shell is provided with the sample dripping hole 7, the central position of the lower shell is provided with the light hole 8, and the diffusion layer 2, the reagent layer 3, the resin layer 4 and the support layer 5 are sequentially arranged between the upper shell 1 and the lower shell 6 from top to bottom and are sequentially attached together in a layered manner from top to bottom through a coating process.
Further, the invention is comprised of a module containing dry chemical reagents attached to a rigid plastic strip. The color of the module changes due to the reaction between various chemical examination contents in the blood and dry chemical reagents, and the color depth or the color change speed of the module is in proportional relation with the concentration of corresponding chemical components in the blood and urine.
The total protein multilayer film dry sheet can be used for quantitatively determining the total protein concentration in serum and plasma. The total protein dry plate is a multilayer analytical component coated on a polyester substrate. The analytical method is based on biuret reaction. When the protein reacts with cupric ions in an alkaline medium, the biuret reaction produces a purple compound, and the sample is dropped through the sample dropping hole of the upper housing onto the surface of the diffusion layer and is uniformly distributed through the diffusion layer to the underlying reagent layer. As the sample solution passes through the reagent layer, the reagent disperses into the diffusion layer and reacts with the protein. The reaction between the protein and the copper tartrate takes place mainly in the diffusion layer; because the protein has a high molecular weight, the protein is bound in this layer. The amount of colored compound produced is determined using reflectance photometry and is proportional to the total protein concentration in the sample. The specific reaction steps are as follows:
the diffusion layer mainly comprises hydrophilic high polymer materials and white reflecting materials, the hydrophilic high polymer materials comprise cellulose acetate, carboxymethyl cellulose and the like, the white reflecting materials comprise titanium dioxide, barium sulfate, polystyrene, microcrystalline cellulose or mixtures of the above reflecting materials, and the reflecting materials mainly provide a background for reflection measurement; the diffusion layer has the main functions of uniformly diffusing a sample, filtering macromolecular substances and interfering substances in the sample and protecting a reagent layer; the wet film thickness of the diffusion layer is 300-500 μm, and the dry film thickness is 70-300 μm, and the effect is better within 100-250 μm.
The reagent layer is a region where a substance to be detected is subjected to a chemical reaction, and the reagent layer comprises sodium potassium tartrate, copper salt, an alkaline compound having a pH value exceeding about 12.0 under the use condition of the elements, and an alkali-resistant polymer. Potassium sodium tartrate is one of the constituents of the biuret component of the reagent layer and may include other salts of tartaric acid, such as sodium tartrate, potassium tartrate, ammonium tartrate or lithium tartrate; copper salts include aqueous copper salts such as copper sulfate, copper nickel triacylate, copper chloride, copper bromide, copper iodide, copper acetate, and the like. Basic compounds having a pH of more than 12.0 include sodium hydroxide, potassium hydroxide, lithium hydroxide, calcium hydroxide, and the like; examples of the alkali-resistant polymer include agarose, starch, polyvinyl pyrrolidone, polyvinyl pyridine, methyl cellulose, methylhydroxypropyl cellulose, polyvinyl alcohol, and the like. The wet film thickness of the reagent layer is 100-300 μm, the dry film thickness is 40-200 μm, and the effect is better within 50-150 μm.
The supporting layer can be made of various transparent plastics, such as Polyethylene (PE), polypropylene (PP), Polyester Methyl Methacrylate (PMMA), Polystyrene (PS), Polycarbonate (PC), polyethylene terephthalate (PET), and the like, preferably PET; the thickness of the supporting layer is 30-200 μm, preferably 50-150 μm; the light transmittance of the visible light range is more than 85 percent, the optimal light transmittance is more than 95 percent, and the incident light source and the reflected light can both pass through the supporting layer. The supporting layer and the reagent layer coating film can be better adhered together by means of the resin layer with the adhesion function, so that the requirements on the material and the performance of the base material are reduced, and the cost is saved.
The resin layer is a novel resin system which takes water as a dispersion medium instead of an organic solvent, and comprises epoxy resin, alkyd resin, polyester resin, polyurethane resin, modified polybutadiene resin and the like, preferably modified polybutadiene resin; the wet film thickness of the resin layer is 10 to 100 μm, and the dry film thickness is 2 to 50 μm, and the effect is better within 10 to 20 μm. The shell mainly comprises the plastics material, the shell is in the same place through the laminating of supersound laminating mode from top to bottom, be fixed in between the shell from top to bottom with the reagent, it is mainly that the protection reagent is in the transportation, store, the in-process of test is because the deformation that various power leads to, thereby avoid reagent to damage, especially in last quick-witted test, no matter be automatic or semi-automatic send into the detection machine with the dry piece reagent, the dry piece inevitable receives corresponding power, the reagent shell can avoid the reagent dry piece damaged.
The preparation method of the dry tablet reagent comprises the following steps:
1. the resin solution (purchased directly) was uniformly coated on a substrate, dried at 45 ℃ for 10min to form a resin layer having a uniform thickness.
2. The following reagent layer solutions were further applied to the dried resin layer, dried at 45 ° for 30min, and dried to form a reagent layer. The formula of the reagent layer solution is as follows:
3. and continuously coating the following diffusion layer solution on the dried reagent layer, drying for 5 hours at the temperature of 25 ℃, and completely drying to finish the manufacture of the dry powder dry sheet. The formulation of the diffusion layer solution was as follows:
4. cutting the dry powder into a certain size, placing the dry powder into a dry sheet shell matched with a machine, and sealing and storing to finish the manufacture of the dry sheet.
The dry chemical reagent prepared by the method has the advantages of excellent performance, stable reagent, long storage time and convenient carrying, and completely realizes the target of immediate detection.
The dry piece detection operation flow is as follows:
1. taking out the dry slices according to the required amount, and returning the temperature for 1 h;
2. the dried tablets were loaded into the test machine and 10ul of whole blood/plasma/urine/cerebrospinal fluid was added drop wise directly to the sample drop on the upper housing of the dried tablet and tested for 5 minutes to yield a result.
And (5) judging a result: referring to the reference value range, the out-of-range is an abnormal value.
In conclusion, the dry chemical method multi-layer membrane reagent dry tablet for in vitro diagnosis provided by the invention has the advantages of rapid diagnosis, simplicity in operation, cost saving and the like, can improve the accuracy, precision and sensitivity of detection, and has traceability, stable reagent and long storage time.
The above description is only an example of the present invention, and is not intended to limit the present invention, and it is obvious to those skilled in the art that various modifications and variations can be made in the present invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the scope of the claims of the present invention.
Claims (9)
1. A total protein quantitative determination dry plate for in vitro diagnosis comprises an upper shell (1) with a sample dripping hole, a diffusion layer (2), a reagent layer (3), a resin layer (4), a support layer (5) and a lower shell (6) with a light hole, it is characterized in that an upper shell (1) and a lower shell (6) are mutually attached, the central position of the upper shell is provided with a sample dripping hole (7), the central position of the lower shell is provided with a light hole (8), a diffusion layer (2), a reagent layer (3), a resin layer (4) and a support layer (5) are sequentially arranged between the upper shell (1) and the lower shell (6) from top to bottom, the reagent layer (3) is a region where the substances to be detected are subjected to chemical reaction, the reagent layer (3) comprises sodium potassium tartrate, copper salt, an alkaline compound and an alkaline-resistant polymer.
2. The dry sheet for quantitative determination of total protein for in vitro diagnosis according to claim 1, wherein the diffusion layer (2) is mainly composed of hydrophilic polymer material and white reflective material, the hydrophilic polymer material is cellulose acetate or carboxymethyl cellulose, the white reflective material is one or a mixture of titanium dioxide, barium sulfate, polystyrene and microcrystalline cellulose, the wet film thickness of the diffusion layer is 300-500 μm, and the dry film thickness is 70-300 μm.
3. The quantitative total protein detection dry plate for in vitro diagnosis of claim 1, wherein the potassium sodium tartrate is tartrate, and the tartrate comprises sodium tartrate, potassium tartrate, ammonium tartrate or lithium tartrate; the copper salt is aqueous copper salt, including copper sulfate, copper nickel triacylate, copper chloride, copper bromide, copper iodide or copper acetate; the pH value of the alkaline compound is more than 12.0, and the alkaline compound comprises sodium hydroxide, potassium hydroxide, lithium hydroxide or calcium hydroxide; the alkali-resistant polymer comprises agarose, starch, polyvinyl pyrrolidone, polyvinyl pyridine, methyl cellulose, methyl hydroxypropyl cellulose or polyvinyl alcohol.
4. The quantitative determination dry plate for total protein for in vitro diagnosis according to claim 1, wherein the wet film thickness of the reagent layer (3) is 100 to 300 μm, and the dry film thickness is 40 to 200 μm.
5. The dry plate for quantitative determination of total protein in vitro diagnosis according to claim 1, wherein the material of the support layer (5) can be made of various transparent plastics, the thickness of the support layer is 30-200 μm, the visible light transmittance of the support layer is above 85%, and both the incident light source and the reflected light can pass through the support layer.
6. The quantitative determination dry plate for total protein in vitro diagnosis according to claim 5, wherein the transparent plastic is made of polyethylene PE, polypropylene PP, polyester methyl methacrylate PMMA, polystyrene PS, polycarbonate PC or polyethylene terephthalate PET.
7. The dry sheet for quantitative determination of total protein for in vitro diagnosis according to claim 1, wherein the resin layer (4) is disposed between the support layer and the reagent layer coating film, and the support layer and the reagent layer are adhered together by the resin layer having an adhesive effect, wherein the resin layer is a novel resin system using water instead of an organic solvent as a dispersion medium.
8. The dry sheet for quantitative determination of total protein for in vitro diagnosis according to claim 7, wherein the resin layer (4) is made of one of epoxy resin, alkyd resin, polyester resin, polyurethane resin and modified polybutadiene resin, and has a wet film thickness of 10 to 100 μm and a dry film thickness of 2 to 50 μm.
9. The dry plate for quantitative determination of total protein for in vitro diagnosis according to claim 1, wherein said upper housing (1) and said lower housing (6) are mainly made of plastic material, and the upper and lower housing layers are attached together by means of ultrasonic bonding.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911055270.1A CN110794146A (en) | 2019-10-31 | 2019-10-31 | Total protein quantitative detection dry tablet for in vitro diagnosis |
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| CN201911055270.1A CN110794146A (en) | 2019-10-31 | 2019-10-31 | Total protein quantitative detection dry tablet for in vitro diagnosis |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113702465A (en) * | 2020-05-20 | 2021-11-26 | 无锡锦帛诚医疗器械科技有限公司 | Potassium ion quantitative detection dry tablet for in vitro diagnosis and preparation method thereof |
| CN113702468A (en) * | 2020-05-20 | 2021-11-26 | 无锡锦帛诚医疗器械科技有限公司 | Chloride ion quantitative detection dry sheet for in vitro diagnosis |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4435362A (en) * | 1981-11-17 | 1984-03-06 | Fuji Shashin Film Kabushiki Kaisha | Integral multilayer analytical element for the assay of total protein |
| CN103630535A (en) * | 2013-12-03 | 2014-03-12 | 上海科华生物工程股份有限公司 | Dry chemical test paper for quantitatively determining content of human blood albumin |
| CN106645763A (en) * | 2017-01-03 | 2017-05-10 | 长沙中生众捷生物技术有限公司 | Total cholesterol detection reagent and total cholesterol detection paper |
| CN110095592A (en) * | 2019-05-05 | 2019-08-06 | 北京石油化工学院 | A kind of c reactive protein monofilm detection dry plate |
| CN110172496A (en) * | 2019-05-28 | 2019-08-27 | 北京石油化工学院 | A kind of Multilayer film dry plate for surveying hdl concentration |
-
2019
- 2019-10-31 CN CN201911055270.1A patent/CN110794146A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4435362A (en) * | 1981-11-17 | 1984-03-06 | Fuji Shashin Film Kabushiki Kaisha | Integral multilayer analytical element for the assay of total protein |
| CN103630535A (en) * | 2013-12-03 | 2014-03-12 | 上海科华生物工程股份有限公司 | Dry chemical test paper for quantitatively determining content of human blood albumin |
| CN106645763A (en) * | 2017-01-03 | 2017-05-10 | 长沙中生众捷生物技术有限公司 | Total cholesterol detection reagent and total cholesterol detection paper |
| CN110095592A (en) * | 2019-05-05 | 2019-08-06 | 北京石油化工学院 | A kind of c reactive protein monofilm detection dry plate |
| CN110172496A (en) * | 2019-05-28 | 2019-08-27 | 北京石油化工学院 | A kind of Multilayer film dry plate for surveying hdl concentration |
Non-Patent Citations (2)
| Title |
|---|
| 孙艳虹等: "干湿化学法测定血浆总蛋白的方法学比较", 《实用医学杂志》 * |
| 徐晓杰等: "总蛋白干化学法测定消除脂浊的干扰", 《温州医学院学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113702465A (en) * | 2020-05-20 | 2021-11-26 | 无锡锦帛诚医疗器械科技有限公司 | Potassium ion quantitative detection dry tablet for in vitro diagnosis and preparation method thereof |
| CN113702468A (en) * | 2020-05-20 | 2021-11-26 | 无锡锦帛诚医疗器械科技有限公司 | Chloride ion quantitative detection dry sheet for in vitro diagnosis |
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Application publication date: 20200214 |