CN110788025A - Fluorescence spectrum cocoon selection method - Google Patents
Fluorescence spectrum cocoon selection method Download PDFInfo
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- CN110788025A CN110788025A CN201911112373.7A CN201911112373A CN110788025A CN 110788025 A CN110788025 A CN 110788025A CN 201911112373 A CN201911112373 A CN 201911112373A CN 110788025 A CN110788025 A CN 110788025A
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- cocoon
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B07—SEPARATING SOLIDS FROM SOLIDS; SORTING
- B07C—POSTAL SORTING; SORTING INDIVIDUAL ARTICLES, OR BULK MATERIAL FIT TO BE SORTED PIECE-MEAL, e.g. BY PICKING
- B07C5/00—Sorting according to a characteristic or feature of the articles or material being sorted, e.g. by control effected by devices which detect or measure such characteristic or feature; Sorting by manually actuated devices, e.g. switches
- B07C5/34—Sorting according to other particular properties
- B07C5/342—Sorting according to other particular properties according to optical properties, e.g. colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N21/643—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" non-biological material
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
- G01N2021/6421—Measuring at two or more wavelengths
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- Optics & Photonics (AREA)
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
A fluorescence spectrum cocoon selection method comprises the following steps: (1) wavelength adjustment: flatly paving the fresh cocoons to be sorted on a fluorescence cocoon sorting table, adjusting the excitation wavelength between 345nm and 550nm, and locking the excitation wavelength when each fresh cocoon on the fluorescence cocoon sorting table shows fluorescence; (2) primary cocoon selection: the workers respectively sieve out the fresh cocoons which obviously show yellow, purple and blue colors; (3) enhancing wavelength: and readjusting the excitation wavelength between 480nm and 600nm, and locking the excitation wavelength when each fresh cocoon on the fluorescence cocoon selection table shows obvious fluorescence after irradiating the remaining fresh cocoons after the primary cocoon selection is finished.
Description
Technical Field
The invention relates to the technical field of cocoon selection, in particular to a fluorescence spectrum cocoon selection method.
Background
Cocoon selection refers to a process for selecting, picking and classifying raw material cocoons according to silk making process requirements. Because the silkworm has different constitutions and environments during cocooning, and is influenced by cocoon collection, cocoon drying, transportation and the like, some inferior cocoons which are difficult to reel high-grade raw silk, cocoons which cannot be reeled, double-cocoon cocoons in which two or more silkworm chrysalis are arranged in cocoons, and the like are often found. Even if the cocoons are of the same variety, the cocoon shapes and sizes, the cocoon shells have different thicknesses, colors and the like. Therefore, cocoon selection and classification must be carried out according to different process requirements so as to meet the requirements of reeling silk.
At present, China stipulates that a dry shell quantity method is adopted to test fresh cocoons in cocoon purchasing, but the method is complex and time-consuming, and cocoon cutting measurement is needed, and cocoons with cut edges become secondary cocoons in the silk reeling industry, so that high-quality silk cannot be reeled, and great waste is caused. The common visual hand feeling measurement method is easily influenced by personal experience of an evaluator, and the measurement precision is low.
According to the phenomenon, people have found that the fluorescent color of the silkworm cocoon corresponds to the internal quality and cocoon layer quantity of the silkworm cocoon, the fluorescent color is the best yellow, the second purple and the worst blue, and the quality grade of the silkworm cocoon can be deduced by identifying the fluorescent color of the silkworm cocoon according to the relationship.
Because the traditional silkworm cocoon quality detection technology restricts the improvement of the quality and the benefit of the silk industry in China, a method for rapidly, accurately and nondestructively detecting the cocoon layer quantity of the silkworm cocoons is urgently required to be found.
Disclosure of Invention
The invention aims to provide a fluorescence spectrum cocoon selection method for quickly, accurately and nondestructively detecting cocoon shell quantity of silkworm cocoons.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a fluorescence spectrum cocoon selection method comprises the following steps:
(1) wavelength adjustment: flatly paving the fresh cocoons to be sorted on a fluorescence cocoon sorting table, adjusting the excitation wavelength between 345nm and 550nm, and locking the excitation wavelength when each fresh cocoon on the fluorescence cocoon sorting table shows fluorescence;
(2) primary cocoon selection: the workers respectively sieve out the fresh cocoons which obviously show yellow, purple and blue colors;
(3) enhancing wavelength: readjusting the excitation wavelength between 480nm and 600nm, and locking the excitation wavelength when each fresh cocoon on the fluorescence cocoon selection table shows obvious fluorescence after irradiating the remaining fresh cocoons after the primary cocoon selection is finished;
(4) and (3) secondary cocoon selection: and (4) screening the remaining fresh cocoons according to yellow, purple and blue by the staff according to detection data given by the fluorescence cocoon selection table.
The fluorescence cocoon selection table comprises a cocoon selection glass table top, a cocoon selection table rack, a frequency-adjustable light source, a fluorescence collection device, a beam splitter, optical filters, an amplifying circuit, a detector, a data processor and a display, wherein the cocoon selection glass table top is installed on the cocoon selection table rack, the frequency-adjustable light source is arranged on the bottom surface of the cocoon selection table rack, the beam splitter is positioned above the cocoon selection glass table top, the output ends of the two sides of the beam splitter are respectively provided with the optical filters, the detector is installed behind the optical filters, the output of the detector is connected with the input of the amplifying circuit, the output of the amplifying circuit is connected with the data processor, and the data processor is connected with the.
The passing wavelengths of the optical filter are 440nm and 540nm respectively.
The working principle is as follows:
the light emitted by the light source irradiates the surface of the silkworm cocoon, the fluorescence emitted by the silkworm cocoon is divided into two beams of light by the beam splitter, the light with the wavelength of 440nm and the light with the wavelength of 540nm are respectively filtered by the two optical filters, the two optical filters are respectively used for detection, the signal received by the detector is amplified by the amplifying circuit and then is sent to the data processor for analysis, and finally the signal is displayed on the display for distinguishing by workers.
The invention has the advantages that:
(1) the method and the device have the advantages that the characteristic that the silkworm cocoons display fluorescence under the illumination of the special wavelength is utilized, the adjustable light source is adopted to adjust the frequency of the fresh cocoons needing to be screened at present, and the accuracy is guaranteed.
(2) The fluorescent data that this application adopted the display to show the detector to gather is convenient for operating personnel to judge, eliminates the error that pure people's eye discernment produced, improves staff's work efficiency.
(3) The fluorescence data are divided into two paths by the beam splitter and collected respectively, the two most stable wavelengths of the fluorescence reaction of the silkworm cocoons obtained through experiments are adopted by the optical filter respectively, and the judgment is combined to improve the accuracy.
Drawings
FIG. 1 is a front view of the fluorescence cocoon selection station;
FIG. 2 is a schematic view of the top structure of the cocoon selection table frame of the fluorescence cocoon selection table;
the meanings of the labels in the figure are as follows:
1-selecting cocoon glass table; 2-cocoon selection table rack; 3-a frequency-tunable light source; 4-a beam splitter; 5-a filter; 6-an amplifying circuit; 7-a detector; 8-a data processor; 9-display.
Detailed Description
Example 1
A fluorescence spectrum cocoon selection method comprises the following steps:
(1) wavelength adjustment: flatly paving the fresh cocoons to be sorted on a fluorescence cocoon sorting table, adjusting the excitation wavelength at 345nm, and locking the excitation wavelength when each fresh cocoon on the fluorescence cocoon sorting table shows fluorescence;
(2) primary cocoon selection: the workers respectively sieve out the fresh cocoons which obviously show yellow, purple and blue colors;
(3) enhancing wavelength: readjusting the excitation wavelength to be 480nm, and locking the excitation wavelength when each fresh cocoon on the fluorescence cocoon selection table shows obvious fluorescence after irradiating the remaining fresh cocoons after primary cocoon selection;
(4) and (3) secondary cocoon selection: and (4) screening the remaining fresh cocoons according to yellow, purple and blue by the staff according to detection data given by the fluorescence cocoon selection table.
Preferably, the fluorescence cocoon selection table comprises a cocoon selection glass table top 1, a cocoon selection table rack 2, a frequency-adjustable light source 3, a beam splitter 4, a light filter 5, an amplifying circuit 6, a detector 7, a data processor 8 and a display 9, wherein the cocoon selection glass table top 1 is installed on the cocoon selection table rack 2, the frequency-adjustable light source 3 is arranged on the bottom surface of the cocoon selection table rack 2, the beam splitter 4 is positioned above the cocoon selection glass table top 1, the light filters 5 are respectively arranged at output ends of two sides of the beam splitter 4, the detector 7 is installed behind the light filter 5, the output of the detector 7 is connected with the input of the amplifying circuit 6, the output of the amplifying circuit 6 is connected with the data processor 8, and the data processor 8 is connected with the display 9.
Preferably, the pass wavelengths of the optical filter 5 are 440nm and 540nm, respectively.
Example 2
A fluorescence spectrum cocoon selection method comprises the following steps:
(1) wavelength adjustment: flatly paving the fresh cocoons to be sorted on a fluorescence cocoon sorting table, adjusting the excitation wavelength at 460nm, and locking the excitation wavelength when each fresh cocoon on the fluorescence cocoon sorting table shows fluorescence;
(2) primary cocoon selection: the workers respectively sieve out the fresh cocoons which obviously show yellow, purple and blue colors;
(3) enhancing wavelength: readjusting the excitation wavelength to be 550nm, and locking the excitation wavelength when each fresh cocoon on the fluorescence cocoon selection table shows obvious fluorescence after irradiating the remaining fresh cocoons after primary cocoon selection;
(4) and (3) secondary cocoon selection: and (4) screening the remaining fresh cocoons according to yellow, purple and blue by the staff according to detection data given by the fluorescence cocoon selection table.
Preferably, the fluorescence cocoon selection table comprises a cocoon selection glass table top 1, a cocoon selection table rack 2, a frequency-adjustable light source 3, a beam splitter 4, a light filter 5, an amplifying circuit 6, a detector 7, a data processor 8 and a display 9, wherein the cocoon selection glass table top 1 is installed on the cocoon selection table rack 2, the frequency-adjustable light source 3 is arranged on the bottom surface of the cocoon selection table rack 2, the beam splitter 4 is positioned above the cocoon selection glass table top 1, the light filters 5 are respectively arranged at output ends of two sides of the beam splitter 4, the detector 7 is installed behind the light filter 5, the output of the detector 7 is connected with the input of the amplifying circuit 6, the output of the amplifying circuit 6 is connected with the data processor 8, and the data processor 8 is connected with the display 9.
Preferably, the pass wavelengths of the optical filter 5 are 440nm and 540nm, respectively.
Example 3
A fluorescence spectrum cocoon selection method comprises the following steps:
(1) wavelength adjustment: flatly paving the fresh cocoons to be sorted on a fluorescence cocoon sorting table, adjusting the excitation wavelength at 550nm, and locking the excitation wavelength when each fresh cocoon on the fluorescence cocoon sorting table shows fluorescence;
(2) primary cocoon selection: the workers respectively sieve out the fresh cocoons which obviously show yellow, purple and blue colors;
(3) enhancing wavelength: readjusting the excitation wavelength to be 600nm, and locking the excitation wavelength when each fresh cocoon on the fluorescence cocoon selection table shows obvious fluorescence after irradiating the remaining fresh cocoons after primary cocoon selection;
(4) and (3) secondary cocoon selection: and (4) screening the remaining fresh cocoons according to yellow, purple and blue by the staff according to detection data given by the fluorescence cocoon selection table.
Preferably, the fluorescence cocoon selection table comprises a cocoon selection glass table top 1, a cocoon selection table rack 2, a frequency-adjustable light source 3, a beam splitter 4, a light filter 5, an amplifying circuit 6, a detector 7, a data processor 8 and a display 9, wherein the cocoon selection glass table top 1 is installed on the cocoon selection table rack 2, the frequency-adjustable light source 3 is arranged on the bottom surface of the cocoon selection table rack 2, the beam splitter 4 is positioned above the cocoon selection glass table top 1, the light filters 5 are respectively arranged at output ends of two sides of the beam splitter 4, the detector 7 is installed behind the light filter 5, the output of the detector 7 is connected with the input of the amplifying circuit 6, the output of the amplifying circuit 6 is connected with the data processor 8, and the data processor 8 is connected with the display 9.
Preferably, the pass wavelengths of the optical filter 5 are 440nm and 540nm, respectively.
Claims (3)
1. A fluorescence spectrum cocoon selection method is characterized by comprising the following steps:
(1) wavelength adjustment: flatly paving the fresh cocoons to be sorted on a fluorescence cocoon sorting table, adjusting the excitation wavelength between 345nm and 550nm, and locking the excitation wavelength when each fresh cocoon on the fluorescence cocoon sorting table shows fluorescence;
(2) primary cocoon selection: the workers respectively sieve out the fresh cocoons which obviously show yellow, purple and blue colors;
(3) enhancing wavelength: readjusting the excitation wavelength between 480nm and 600nm, and locking the excitation wavelength when each fresh cocoon on the fluorescence cocoon selection table shows obvious fluorescence after irradiating the remaining fresh cocoons after the primary cocoon selection is finished;
(4) and (3) secondary cocoon selection: and (4) screening the remaining fresh cocoons according to yellow, purple and blue by the staff according to detection data given by the fluorescence cocoon selection table.
2. The fluorescence spectrum cocoon selection method according to claim 1, characterized in that: the fluorescence cocoon selection table comprises a cocoon selection glass table board (1), a cocoon selection table rack (2), a frequency-adjustable light source (3), a beam splitter (4), an optical filter (5), an amplifying circuit (6), a detector (7), a data processor (8) and a display (9), wherein the cocoon selection glass table board (1) is installed on the cocoon selection table rack (2), the frequency-adjustable light source (3) is arranged on the bottom surface of the cocoon selection table rack (2), the beam splitter (4) is located at the output ends of the two sides of the beam splitter (4) above the cocoon selection glass table board (1), the detector (7) is installed behind the optical filter (5), the output of the detector (7) is connected with the input of the amplifying circuit (6), the output of the amplifying circuit (6) is connected with the data processor (8), and the data processor (8) is connected with the display (9).
3. The fluorescence spectrum cocoon selection method according to claim 2, characterized in that: the passing wavelengths of the optical filter (5) are 440nm and 540nm respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911112373.7A CN110788025A (en) | 2019-11-14 | 2019-11-14 | Fluorescence spectrum cocoon selection method |
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| CN201911112373.7A CN110788025A (en) | 2019-11-14 | 2019-11-14 | Fluorescence spectrum cocoon selection method |
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Citations (9)
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2019
- 2019-11-14 CN CN201911112373.7A patent/CN110788025A/en active Pending
Patent Citations (9)
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| TW272150B (en) * | 1994-05-31 | 1996-03-11 | Coca Cola Co | |
| CN1354627A (en) * | 1999-06-08 | 2002-06-19 | 日本烟草产业株式会社 | Apparatus for detecting foreign matter in raw material and method of detecting the same |
| US6734383B1 (en) * | 1999-06-28 | 2004-05-11 | Barco Elbicon, Naamloze Vennootschap | Method and device for sorting products according to emitted light |
| CN101054731A (en) * | 2007-05-21 | 2007-10-17 | 苏州大学 | Method for preparing high grade sexual silk |
| CN102253025A (en) * | 2011-06-22 | 2011-11-23 | 中国人民公安大学 | Composite fluorescence imaging system applied in forensic science evidence testing, and application method thereof |
| CN105874334A (en) * | 2013-11-18 | 2016-08-17 | 硕腾服务有限责任公司 | Non-contact egg identification system for determining egg viability using transmission spectroscopy, and associated method |
| CN104646314A (en) * | 2015-02-02 | 2015-05-27 | 南昌大学 | Method for screening LED core particles |
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