CN110786334A - Nano pesticide for preventing and controlling monochamus alternatus and pine wood nematodes - Google Patents
Nano pesticide for preventing and controlling monochamus alternatus and pine wood nematodes Download PDFInfo
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- A—HUMAN NECESSITIES
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
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- A—HUMAN NECESSITIES
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- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
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- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/22—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients stabilising the active ingredients
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Abstract
The invention discloses a novel nano pesticide for preventing and treating monochamus alternatus and pine wood nematodes, which is prepared by loading pesticide avermectin on a nano Cu-BTC carrier to obtain a nano pesticide preparation; wherein, the nano Cu-BTC as the carrier has uniform and stable grain diameter, good dispersibility and no agglomeration; and the inside of the nano Cu-BTC is of a porous structure, so that the nano Cu-BTC has a large specific surface area, high drug loading and good sustained and controlled release effect. The nano pesticide preparation prepared by the invention can penetrate through the cell wall barrier of plants due to the nano size, and the medicine is transported into the bark for preventing and controlling Monochamus alternatus and pine wood nematodes.
Description
Technical Field
The invention relates to the technical field of pesticide preparations, in particular to a novel nano pesticide for preventing and treating monochamus alternatus hope and pine wood nematode and a prevention and treatment method thereof.
Background
The pine wood nematode is one of the foreign invasive species with great harm in China and is also the pathogen of pine wilt. The water transportation, photosynthesis, respiration and transpiration of the pine trees infected by the pine wood nematodes are inhibited, the bark of the pine trees becomes dry, the needles begin to fade green and become brown, and the pine trees gradually wither to die, so that huge economic loss is caused to the forestry in China. As a main propagation medium of the pine wood nematodes, monochamus alternatus has also attracted wide attention, and not only can adult monochamus alternatus propagate the pine wood nematodes in the feeding process, but also larvae of the monochamus alternatus can eat cortex and sapwood of weak pine trees to cause irregular flat tunnels and then eat xylem of people, so that the pine trees gradually die. At present, no good control measures are provided for monochamus alternatus larvae, adult monochamus alternatus gnaws leaves, a more advanced treatment method is to spray a microcapsule mixture through an unmanned aerial vehicle, so that a better contact killing effect is achieved, and the problems of unstable microcapsule form, obvious burst release phenomenon and the like bring obstacles to the control of monochamus alternatus adults. The existing control method for the pine wood nematodes is to inject pesticides into a trunk in an injection mode, and most pesticides are hydrophobic, so that the pesticides are difficult to transport in the trunk, and stomach toxicity or contact poisoning of the pine wood nematodes cannot be realized. Therefore, there is an urgent need for an effective method for controlling monochamus alternatus and pine wood nematodes together.
The nano pesticide is a pesticide preparation product which is created by efficiently mixing a raw pesticide, a carrier and an auxiliary agent by utilizing a nano material and a preparation technology. The pesticide particles are reduced from 5 microns to 100nm by utilizing the nanotechnology, and the function of the pesticide is fully exerted. Compared with the traditional pesticide formulation, the nano pesticide has smaller size and larger specific surface area, thereby having better dispersibility, stronger adsorption force and better utilization rate. In addition, the carrier of the nano pesticide can improve the stability of the pesticide molecules in the environment by encapsulating the pesticide molecules. However, since the frictional force between nanoparticles (the frictional force is due to electrostatic force, van der waals force and surface adsorption force) is very significant, the nanoparticles contain a large number of pores and have a large specific surface area, so that they are more prone to agglomeration or coagulation, and therefore, it is extremely difficult to maintain the size of the nanoparticles to be stable, which restricts the research and development of nano-pesticides.
Avermectin (Avermectin) is a hexadecimal macrolide compound separated from Streptomyces avermitilis fermentation liquor separated from soil samples of Kitasato research in Japan, has the characteristics of broad spectrum, high efficiency and low residue, is commonly used as a pesticide, a bactericide and a nematicide, and has stomach toxicity and contact poisoning effects on coleoptera, lepidoptera and other pests. However, abamectin is difficult to dissolve in water, and the light degradation half-life period in water and soil is 12h and 21h respectively, so that abamectin is a substance easy to photolysis. At present, abamectin is generally prepared into microcapsules for preventing and treating pine wood nematodes (CN106035356A), but the microcapsules still have serious agglomeration phenomenon.
Disclosure of Invention
Aiming at the prior art, the invention aims to provide a novel nano pesticide for preventing and treating monochamus alternatus and pine wood nematodes, which effectively solves the problems that the traditional pesticide preparation has low pesticide effect and poor controllability, cannot kill pests in a trunk, is easy to generate drug resistance and the like.
In order to achieve the purpose, the invention adopts the following technical scheme:
in a first aspect of the invention, the use of nano Cu-BTC as a carrier in the preparation of nano pesticide formulations is provided.
In the above application, the size of the nano Cu-BTC is preferably 40-90 nm.
In the above application, preferably, the nano Cu-BTC is prepared by the following method:
dissolving copper acetate and benzoic acid in n-butyl alcohol, and stirring at room temperature until the copper acetate and the benzoic acid are dissolved to obtain a first solution; dissolving trimesic acid in tetrahydrofuran, and stirring at room temperature until the trimesic acid is dissolved to obtain a second solution; dropwise stirring the second solution and adding the second solution into the first solution, and reacting at room temperature for 6-24 h; and after the reaction is finished, centrifuging to obtain a precipitate, washing and drying to obtain the nano Cu-BTC.
More preferably, the concentration of copper acetate in the first solution is 3.0-4.0g/L and the concentration of benzoic acid is 8.0-40.0 g/L.
More preferably, the concentration of trimesic acid in the second solution is 10.0 to 40.0 g/L.
More preferably, the first solution and the second solution are added in a volume ratio of 1:1 to 5: 1.
In a second aspect of the present invention, there is provided a nano pesticide preparation, which is prepared by the steps of:
(1) dispersing the nano Cu-BTC into tetrahydrofuran to obtain nano Cu-BTC dispersion liquid with the concentration of 0.01-0.4 mg/ml;
(2) dissolving abamectin in tetrahydrofuran to obtain an abamectin solution with the concentration of 0.01-0.4 mg/ml;
(3) and stirring the abamectin solution, adding the abamectin solution into the nano Cu-BTC dispersion liquid, continuously stirring for 24-72 hours, centrifuging, collecting the precipitate, and drying to obtain the nano pesticide preparation.
Preferably, in the step (3), the volume ratio of the abamectin solution to the nano Cu-BTC dispersion liquid is 1:100-1: 5.
In a third aspect of the invention, the application of the nano pesticide preparation in controlling monochamus alternatus and pine wood nematodes is provided.
In a fourth aspect of the present invention, there is provided a method for controlling monochamus alternatus and pine wood nematodes, comprising the steps of:
dispersing the nano pesticide preparation into water, and spraying by using an unmanned aerial vehicle to control adult monochamus alternatus; or dispersing the nano pesticide preparation into water, injecting the nano pesticide preparation into pine trees in an injection mode, and preventing and treating the pine cone longicorn larvae and the pine wood nematodes.
The invention has the beneficial effects that:
(1) the invention uses the nano Cu-BTC as the carrier to load the pesticide avermectin for the first time to prepare the nano pesticide preparation; wherein, the nano Cu-BTC as the carrier has uniform and stable grain diameter, good dispersibility and no agglomeration; and the inside of the nano Cu-BTC is of a porous structure, so that the nano Cu-BTC has a large specific surface area, high drug loading and good sustained and controlled release effect.
(2) The nano pesticide preparation prepared by the invention can penetrate through the cell wall barrier of plants due to the nano size, and the medicine is transported into the bark for preventing and controlling Monochamus alternatus and pine wood nematodes.
(3) The synthesis method of the nano Cu-BTC is simple, high in yield, low in equipment requirement, free of environmental pollution and wide in market application prospect, and the used organic solvent can be recycled.
(4) After the abamectin is adsorbed into the nano Cu-BTC, the quick photolysis of the abamectin can be effectively prevented, the utilization rate of the medicament is greatly improved, the application times of the medicament are reduced, the reduction of the pesticide is realized, and the environment-friendly effect is realized.
Drawings
FIG. 1 is a scanning electron microscope topography (perspective view) of the nano Cu-BTC loaded avermectin prepared by the invention.
FIG. 2 is a scanning electron microscope topography (enlarged view) of the nano Cu-BTC loaded avermectin prepared by the invention.
FIG. 3 is a particle size distribution diagram of the nano Cu-BTC loaded avermectin prepared by the invention.
FIG. 4 is a comparison of the sustained release effect of nano Cu-BTC loaded avermectin and microcapsule loaded avermectin prepared by the invention; wherein, Microcapsules represent Microcapsules loaded with avermectin (a commercial product); nano Cu-BTC represents Nano Cu-BTC loaded abamectin.
Detailed Description
It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
Description of terms:
the term "room temperature" as used herein means a temperature of 15 ℃ to 25 ℃.
As described in the background section, pine wood nematodes are transmitted by adult Monochamus alternatus, larvae of Monochamus alternatus feeding on the xylem tissue of pine trees are boring pests. At present, an effective prevention and control method for the pine wood nematodes and the pine cone longicorn larvae is lacked, and the pine wood nematodes and the pine cone longicorn larvae are difficult to kill by applying the pesticide from the outside of pine trees; the pesticide is injected into the trunk in an injection mode, but most of pesticides are hydrophobic, are difficult to transport in the trunk, and cannot realize stomach toxicity or contact killing of pine wood nematodes and pine cone beetle larvae.
The existing research shows that the indoor bioassay method for determining the 5 percent abamectin emulsifiable solution has extremely strong poisoning property on the pine wood nematodes, and the application of the abamectin injection dry-application pesticide for preventing the pine wood nematode disease is an effective and simple method for controlling the pine wood nematode disease, and is an effective means for preventing pine with important value from infecting the pine wood nematode disease. However, the avermectin is hydrophobic and is easy to photolyze, so that the current technical difficulty lies in the preparation of the avermectin into any dosage form for preventing and treating the pine wood nematodes.
In order to solve the problems of avermectin hydrophobicity and easy photolysis, the avermectin is prepared into a microcapsule form in the prior art, but the microcapsule is easy to agglomerate, so that the application of the avermectin in the prevention and treatment of the pine wilt disease is restricted.
Based on the nano pesticide, the nano pesticide takes the nano Cu-BTC as a carrier to load the abamectin, can be effectively dispersed in water and can be transported in a trunk, and stomach toxicity or contact killing of the pine wood nematodes and the pine wood longicorn larvae is realized.
The nano Cu-BTC used in the invention is mainly used as an adsorbent and a catalyst at present, and no research is related to the application of the nano Cu-BTC in the production of pesticide preparations, and no report is provided on how to prevent and control Monochamus alternatus and pine wood nematodes by loading pesticide avermectin on the nano Cu-BTC.
The size, structure and charged property of the nano Cu-BTC influence the drug loading rate, slow release effect and dispersion effect. The preparation method of the nano Cu-BTC is optimized, and the types and the proportions of the reaction raw materials and the reaction solvent influence the yield, the size, the structure and the charge of the prepared nano Cu-BTC, so that the types and the dosage of the raw materials and the solvent for preparing the nano Cu-BTC are inspected, the yield of the nano Cu-BTC prepared by the method is more than 98 percent, the size of the nano Cu-BTC is between 40 and 90nm, and the particle size is uniformly dispersed and stable; the Cu-BTC has negative charges, and each nano particle has the same charge repulsion phenomenon, so that the dispersibility of the Cu-BTC is good and no agglomeration phenomenon exists. The Zeta potential can react and disperse, and the Zeta potential absolute value of the nano Cu-BTC prepared by the invention is more than 30mV, so that the nano Cu-BTC has better dispersion stability.
In addition, the nano Cu-BTC prepared by the invention belongs to one of MOF families and is composed of a metal-organic framework, and the obtained nano particles are more stable and have better dispersibility than the conventional polymer nanospheres due to the framework supporting effect of metal ions. Pores with the size of several nanometers in the interior can accommodate a large amount of drug molecules, and the drug loading is high; the nano Cu-BTC is insensitive to light, is not easy to degrade due to light heat and is sensitive to pH, so that the photosensitive drug abamectin coated by the nano Cu-BTC as a carrier can effectively resist the visible light decomposition of the abamectin.
In order to make the technical solutions of the present application more clearly understood by those skilled in the art, the technical solutions of the present application will be described in detail below with reference to specific embodiments.
The test materials used in the examples of the present invention were all commercially available materials that are conventional in the art and are commercially available.
Example 1:
(1) dissolving 0.72g of copper acetate and 8g of benzoic acid in 200mL of n-butanol, and stirring at room temperature until the copper acetate and the benzoic acid are dissolved to obtain a copper acetate-benzoic acid/n-butanol solution;
(2) dissolving 1.6g of trimesic acid in 40mL of tetrahydrofuran, and stirring at room temperature until the trimesic acid is dissolved to obtain a trimesic acid/tetrahydrofuran solution;
(3) dropwise stirring the trimesic acid/tetrahydrofuran solution prepared in the step (2) and adding the solution into the copper acetate-benzoic acid/n-butyl alcohol solution prepared in the step (1), reacting for 6 hours at room temperature, centrifuging at 8000rpm after the reaction is finished to obtain blue precipitate, washing with tetrahydrofuran for multiple times, centrifuging, collecting the precipitate, and drying in vacuum to obtain nano Cu-BTC; the yield of the obtained nano Cu-BTC is 99.0%.
Yield of nano Cu-BTC ═ (actual yield of nano Cu-BTC/theoretical calculated yield of nano Cu-BTC) × 100%.
(4) Dispersing the nano Cu-BTC prepared in the step (3) into tetrahydrofuran to obtain nano Cu-BTC dispersion liquid with the concentration of 0.01 mg/mL; dissolving abamectin in tetrahydrofuran to obtain an abamectin solution with the concentration of 0.01 mg/mL.
Stirring and adding the abamectin solution into the nano Cu-BTC dispersion liquid, wherein the volume ratio of the abamectin solution to the nano Cu-BTC dispersion liquid is 1:40, continuously stirring for 24 hours, centrifuging, collecting precipitate, repeatedly washing with tetrahydrofuran, and drying in vacuum to obtain the novel pesticide of the nano Cu-BTC loaded abamectin.
The obtained novel pesticide has drug-loading rate of 68.8% and average particle diameter of 65 nm.
Wherein the content of the first and second substances,
the mass of the abamectin embedded in the nano Cu-BTC is measured by an ultraviolet spectrophotometer, then the concentration is calculated by using a standard curve, and the mass is converted by multiplying the solution by the volume.
Scanning electron microscopes of the nano Cu-BTC loaded avermectin prepared in the embodiment are shown in fig. 1 and fig. 2, and particle size distribution diagrams of the nano Cu-BTC loaded avermectin are shown in fig. 3.
The sustained release effect of the nano Cu-BTC loaded avermectin prepared in the embodiment is examined by taking a commercially available avermectin microcapsule suspending agent as a control, and the result is shown in FIG. 4, and as can be seen from FIG. 4, compared with the control, the nano Cu-BTC loaded avermectin prepared in the embodiment can realize the long-acting sustained release of the avermectin.
Example 2:
(1) dissolving 1.44g of copper acetate and 4g of benzoic acid in 400mL of n-butanol, and stirring at room temperature until the copper acetate and the benzoic acid are dissolved to obtain a copper acetate-benzoic acid/n-butanol solution;
(2) dissolving 0.8g of trimesic acid in 80mL of tetrahydrofuran, and stirring at room temperature until the trimesic acid is dissolved to obtain a trimesic acid/tetrahydrofuran solution;
(3) dropwise stirring the trimesic acid/tetrahydrofuran solution prepared in the step (2) and adding the solution into the copper acetate-benzoic acid/n-butyl alcohol solution prepared in the step (1), reacting for 12 hours at room temperature, centrifuging at 8000rpm after the reaction is finished to obtain blue precipitate, washing with tetrahydrofuran for multiple times, centrifuging, collecting the precipitate, and drying in vacuum to obtain nano Cu-BTC; the yield of the obtained nano Cu-BTC is 98.6%.
(4) Dispersing the nano Cu-BTC prepared in the step (3) into tetrahydrofuran to obtain nano Cu-BTC dispersion liquid with the concentration of 0.04 mg/mL; dissolving abamectin in tetrahydrofuran to obtain an abamectin solution with the concentration of 0.04 mg/mL.
Stirring and adding the abamectin solution into the nano Cu-BTC dispersion liquid, wherein the volume ratio of the abamectin solution to the nano Cu-BTC dispersion liquid is 1:60, continuously stirring for 48 hours, centrifuging, collecting precipitate, repeatedly washing with tetrahydrofuran, and drying in vacuum to obtain the novel pesticide of the nano Cu-BTC loaded abamectin.
The obtained novel pesticide has drug loading of 70.2% and average particle diameter of 67 nm.
Example 3:
(1) dissolving 3.6g of copper acetate and 8g of benzoic acid in 1000mL of n-butanol, and stirring at room temperature until the copper acetate and the benzoic acid are dissolved to obtain a copper acetate-benzoic acid/n-butanol solution;
(2) dissolving 8g of trimesic acid in 200mL of tetrahydrofuran, and stirring at room temperature until the trimesic acid is dissolved to obtain a trimesic acid/tetrahydrofuran solution;
(3) dropwise stirring the trimesic acid/tetrahydrofuran solution in the step (2) and adding the solution into copper acetate-benzoic acid/n-butyl alcohol solution, reacting for 24 hours at room temperature, centrifuging at 8000rpm after the reaction is finished to obtain blue precipitate, washing the blue precipitate with tetrahydrofuran for multiple times, centrifuging, collecting the precipitate, and drying in vacuum to obtain the nano Cu-BTC; the yield of the obtained nano Cu-BTC is 98.3 percent.
(4) Dispersing the nano Cu-BTC prepared in the step (3) into tetrahydrofuran to obtain nano Cu-BTC dispersion liquid with the concentration of 0.2 mg/mL; dissolving abamectin in tetrahydrofuran to obtain an abamectin solution with the concentration of 0.4 mg/mL.
Stirring and adding the abamectin solution into the nano Cu-BTC dispersion liquid, wherein the volume ratio of the abamectin solution to the nano Cu-BTC dispersion liquid is 1:80, continuously stirring for 72 hours, centrifuging, collecting precipitate, repeatedly washing with tetrahydrofuran, and drying in vacuum to obtain the novel pesticide of the nano Cu-BTC loaded abamectin.
The obtained novel nano pesticide has the drug loading of 71.5 percent and the average particle size of 72 nm.
Comparative example 1:
(1) dissolving 0.36g of copper acetate and 4g of benzoic acid in 200mL of n-butanol, and stirring at room temperature until the copper acetate and the benzoic acid are dissolved to obtain a copper acetate-benzoic acid/n-butanol solution;
(2) dissolving 4g of trimesic acid in 200ml of the solution of the DMF, and stirring at room temperature until the trimesic acid is dissolved to obtain a solution of the trimesic acid/DMF;
(3) dropwise stirring the trimesic acid/DMF solution prepared in the step (2) and adding the solution into copper acetate-benzoic acid/n-butyl alcohol solution, stirring to ensure that the reaction is complete, centrifuging the product, and washing the product for 2 times by using ethanol to obtain the nano Cu-BTC, wherein the yield is 91.2%, and the average particle size is 100 nm.
(4) Dispersing the nano Cu-BTC prepared in the step (3) into DMF to obtain nano Cu-BTC dispersion liquid with the concentration of 0.2 mg/mL; dissolving abamectin in DMF to obtain an abamectin solution with the concentration of 0.4 mg/mL.
Stirring and adding the abamectin solution into the nano Cu-BTC dispersion liquid, wherein the volume ratio of the abamectin solution to the nano Cu-BTC dispersion liquid is 1:40, continuously stirring for 72 hours, centrifuging, collecting precipitate, repeatedly washing with tetrahydrofuran, and drying in vacuum to obtain the novel pesticide of the nano Cu-BTC loaded abamectin.
The obtained novel nano pesticide has drug loading rate of 52.1% and average particle size of 100 nm.
Comparative example 2:
(1) 2gCu (ClO) will be dissolved at normal temperature and pressure4)215ml ofMixing the seed water with 15ml ethanol solution dissolved with 1g trimesic acid, stirring, reacting for 3h to obtain Cu (C)9H4O6)(H2O)3。
(2) Under normal temperature and pressure, 1g of Cu (C)9H4O6)(H2O)3And immersing the precipitate into 20ml of DMF solvent, stirring and reacting for 3min, and washing, filtering and drying the obtained precipitate to obtain the Cu-BTC with the yield of 91.4 percent.
(3) Immersing 1g of Cu-BTC into 50ml of hydrochloric acid solution with pH value of 1.5, reacting and stirring for 10min, filtering, immersing the solid into 50ml of DMF solution, reacting and stirring for 5min, filtering, washing and drying to obtain the nano Cu-BTC, wherein the yield is 84.2%, and the average size is 550 nm.
(4) Dispersing the nano Cu-BTC prepared in the step (3) into DMF to obtain nano Cu-BTC dispersion liquid with the concentration of 0.2 mg/mL; dissolving abamectin in DMF to obtain an abamectin solution with the concentration of 0.4 mg/mL.
Stirring and adding the abamectin solution into the nano Cu-BTC dispersion liquid, wherein the volume ratio of the abamectin solution to the nano Cu-BTC dispersion liquid is 1:40, continuously stirring for 72 hours, centrifuging, collecting precipitate, repeatedly washing with tetrahydrofuran, and drying in vacuum to obtain the novel pesticide of the nano Cu-BTC loaded abamectin.
The drug-loading rate of the obtained nano novel pesticide is 47.2%, and the average particle size is 550 nm.
The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.
Claims (10)
1. The application of the nano Cu-BTC as a carrier in preparing a nano pesticide preparation.
2. Use according to claim 1, characterized in that the size of the nanocu-BTC is 40-90 nm.
3. Use according to claim 1 or 2, characterized in that the nano Cu-BTC is prepared by a process comprising:
dissolving copper acetate and benzoic acid in n-butyl alcohol, and stirring at room temperature until the copper acetate and the benzoic acid are dissolved to obtain a first solution; dissolving trimesic acid in tetrahydrofuran, and stirring at room temperature until the trimesic acid is dissolved to obtain a second solution; dropwise stirring the second solution and adding the second solution into the first solution, and reacting at room temperature for 6-24 h; and after the reaction is finished, centrifuging to obtain a precipitate, washing and drying to obtain the nano Cu-BTC.
4. Use according to claim 3, wherein the concentration of copper acetate in the first solution is between 3.0 and 4.0g/L and the concentration of benzoic acid is between 8.0 and 40.0 g/L.
5. Use according to claim 3, wherein the concentration of trimesic acid in the second solution is between 10.0 and 40.0 g/L.
6. Use according to claim 3, wherein the first and second solutions are added in a volume ratio of 1:1 to 5: 1.
7. A nano pesticide preparation is characterized by being prepared by the following steps:
(1) dispersing the nano Cu-BTC of any one of claims 1 to 6 in tetrahydrofuran to obtain a nano Cu-BTC dispersion with a concentration of 0.01 to 0.4 mg/ml;
(2) dissolving abamectin in tetrahydrofuran to obtain an abamectin solution with the concentration of 0.01-0.4 mg/ml;
(3) and stirring the abamectin solution, adding the abamectin solution into the nano Cu-BTC dispersion liquid, continuously stirring for 24-72 hours, centrifuging, collecting the precipitate, and drying to obtain the nano pesticide preparation.
8. The nano pesticide preparation as claimed in claim 7, wherein in the step (3), the volume ratio of the abamectin solution to the nano Cu-BTC dispersion liquid is 1:100-1: 5.
9. Use of the nanopesticide formulation of claim 7 or 8 for controlling monochamus alternatus and pine wood nematodes.
10. A method for preventing and controlling Monochamus alternatus and pine wood nematodes is characterized by comprising the following steps:
dispersing the nano pesticide preparation of claim 7 or 8 into water, and spraying by using an unmanned aerial vehicle to control adult monochamus alternatus; or, the nano pesticide preparation of claim 7 or 8 is dispersed in water and injected into pine trees by injection to control the larva of Monochamus alternatus and the pine wood nematode.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113994979A (en) * | 2021-10-25 | 2022-02-01 | 山东农业大学 | CuBTC nano pesticide preparation for preventing and treating pine wood nematode and its vector insect Monochamus alternatus |
| CN114052012A (en) * | 2021-10-25 | 2022-02-18 | 山东农业大学 | Preparation of intelligent nano pesticide for preventing and treating pine wood nematode disease and vector insect Monochamus alternatus |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113994979A (en) * | 2021-10-25 | 2022-02-01 | 山东农业大学 | CuBTC nano pesticide preparation for preventing and treating pine wood nematode and its vector insect Monochamus alternatus |
| CN114052012A (en) * | 2021-10-25 | 2022-02-18 | 山东农业大学 | Preparation of intelligent nano pesticide for preventing and treating pine wood nematode disease and vector insect Monochamus alternatus |
| CN115474614A (en) * | 2021-10-25 | 2022-12-16 | 山东农业大学 | CuBTC nano pesticide preparation for preventing and treating pine wood nematode and its vector insect Monochamus alternatus |
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