CN110780003A - Method for measuring content of cannabidiol and tetrahydrocannabinol in industrial cannabis sativa leaves - Google Patents
Method for measuring content of cannabidiol and tetrahydrocannabinol in industrial cannabis sativa leaves Download PDFInfo
- Publication number
- CN110780003A CN110780003A CN201911103733.7A CN201911103733A CN110780003A CN 110780003 A CN110780003 A CN 110780003A CN 201911103733 A CN201911103733 A CN 201911103733A CN 110780003 A CN110780003 A CN 110780003A
- Authority
- CN
- China
- Prior art keywords
- cannabidiol
- tetrahydrocannabinol
- high performance
- performance liquid
- liquid chromatograph
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N30/14—Preparation by elimination of some components
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N2030/042—Standards
- G01N2030/047—Standards external
Abstract
The invention relates to the technical field of detection, and particularly relates to a quantitative detection method for cannabidiol and tetrahydrocannabinol in industrial cannabis sativa leaves. The method comprises the following steps: pulverizing dried hemp flower and leaf, ultrasonic extracting with methanol, centrifuging, collecting supernatant, repeatedly ultrasonic extracting residue with methanol once, centrifuging, mixing supernatants, concentrating to desired volume to obtain solution, and detecting with high performance liquid chromatograph. The mobile phase of the high performance liquid chromatograph is water and acetonitrile, and isocratic elution is carried out. And (4) quantitatively obtaining the content of the cannabidiol and the tetrahydrocannabinol in the liquid to be detected by adopting an external standard method. The method is simple to operate and good in separation degree, and cannabidiol and tetrahydrocannabinol in the cannabis sativa leaves are accurately quantified.
Description
Technical Field
The invention relates to the technical field of detection, and particularly relates to a method for determining the content of cannabidiol and tetrahydrocannabinol in industrial cannabis sativa leaves.
Background
Industrial cannabis refers to cannabis with the content of Tetrahydrocannabinol (THC) lower than 0.3%, China refers to industrial cannabis as hemp, which is an annual herbaceous plant in cannabis of the cannabinaceae, the main active ingredient of cannabinoids is cannabinoids, at present, 70 natural cannabinoids are known, and the cannabinoids are mainly used for multiple sclerosis, motor nerve diseases, chronic intractable pain and drug induced vomiting in certain nervous system diseases, and have certain effects on glaucoma, asthma and cardiovascular diseases. The most common components are Tetrahydrocannabinol (THC) and Cannabidiol (CBD), with different cannabis flowers and leaves containing different amounts of tetrahydrocannabinol and cannabidiol.
In contrast, cannabidiol has a high medicinal value, tetrahydrocannabinol has a neurohallucinogenic effect, can generate hallucinations, is listed as one of three poisons in parallel with heroin and cocaine by the banning convention of the united nations, and becomes a reason for banning cannabis in many countries. With the intensive research on cannabis, cannabidiol is found to block the influence of tetrahydrocannabinol on the nervous system of a human body, has pharmacological activities of resisting spasm, rheumatic arthritis, anxiety and the like, and can effectively improve the sleep quality, relieve nerves, relax mood, relieve pressure and prevent depression. Cannabidiol has wide action and is not limited to single disease species or organ health care. Under the premise of not causing obvious damage to any cognitive system, the cannabidiol has the functions of relieving stress, resisting anxiety, resisting pressure and the like, and simultaneously has obvious positive effects on preventing various diseases such as senile dementia, epilepsy, infantile autism, cancer, liver cirrhosis and the like. In addition to medicinal value, the cannabidiol product is in diversified development, perfectly adapts to the updating and iteration rate of the health product market, and the cannabidiol oil health product extracted from cannabidiol comprises spraying agent, capsule, concentrated solution, ointment, drops and the like, and can be prepared into cannabidiol nutriments with various tastes or concentrations according to the requirements of consumers.
According to authoritative data statistics, about 1% of the world's population may become potential cannabidiol product users at some point in life in the next decade. At present, Cannabidiol (CBD) becomes a new research hotspot of cannabis, and the cannabidiol is mainly extracted from industrial cannabis leaves, so that the accurate determination of the content of the cannabidiol in the flower and leaf raw materials is very important. The industrial hemp permitted to be planted in China is a hemp variety with the content of the whole tetrahydrocannabinol below 0.3%. However, when cannabidiol is extracted, tender leaves and buds with high active cannabinoid content are generally used, and the content of tetrahydrocannabinol generated in the extraction process is often higher than 0.3%. It is also clear from the' permission of planting and processing hemp in labor saving industry of Yunnan, that the content of tetrahydrocannabinol extracted from the processing of flowers and leaves of industrial hemp is higher than 0.3%, and the method is applicable to laws and regulations of drug control. Therefore, in spite of the variety of industrial cannabis grown, concerns remain regarding the content of tetrahydrocannabinol when used to extract cannabidiol. Therefore, it is necessary to develop a detection method capable of accurately measuring the content of tetrahydrocannabinol and cannabidiol in industrial cannabis sativa leaves.
Disclosure of Invention
According to the method, the cannabidiol and the tetrahydrocannabinol in the industrial cannabis sativa leaves are extracted by using methanol ultrasonic waves, the detection is carried out by using a high performance liquid chromatograph, the quantification is carried out by using an external standard method, and the content of the cannabidiol and the tetrahydrocannabinol in the cannabis sativa leaves can be accurately and rapidly detected.
In order to achieve the purpose, the technical scheme of the invention is as follows:
(1) the invention provides a method for measuring the content of cannabidiol and tetrahydrocannabinol in industrial cannabis sativa leaves, which comprises the following steps:
① drying fresh industrial hemp flower and leaf sample in oven at 60 deg.C for 12 hr (water content less than 5%), pulverizing to 20 mesh, and mixing.
② accurately weighing the flower and leaf sample in a centrifuge tube, adding organic solvent, ultrasonic extracting, centrifuging, collecting supernatant, repeatedly ultrasonic extracting the residue once, centrifuging, mixing the supernatants, and concentrating to desired volume to obtain solution to be tested.
③ detecting the liquid to be detected by high performance liquid chromatograph, wherein the mobile phase of the high performance liquid chromatograph is water and acetonitrile, and the liquid is eluted at equal rate.
④ the method comprises preparing reference solutions from standard reference substances of cannabidiol and tetrahydrocannabinol, and measuring the content of cannabidiol and tetrahydrocannabinol in the solution to be measured.
(2) Further, the hemp flower leaves in step ① are a leaf and bud composition of mature industrial hemp harvested at 9 months.
(3) Further, in the step ②, the organic solvent is a chromatographic pure solvent, and is one or a mixture of methanol, ethanol, n-hexane and ethyl acetate, and the mass ratio of the volume amount of the organic solvent to the flower and leaf sample is 5-20: 1.
(4) Preferably, the organic solvent in step ② is chromatographically pure methanol, and the ratio of methanol volume to cannabis sativa leaf mass is 10: 1.
(5) Further, in the step ②, the ultrasonic time is 5 min-30 min.
(6) Preferably, the ultrasonic time in step ② is 20min, the ultrasonic power is 250W, and the working frequency is 40 kHz.
(7) Further, in the step ②, the centrifugal rotation speed is 5000-10000 rpm, and the time is 5-10 minutes.
(8) Preferably, the centrifugation speed in step ② is 10000 rpm, and the centrifugation time is 5 minutes.
(9) Further, in the step ③, the brand model of the high performance liquid chromatograph is Agilent 1260, the mobile phase acetonitrile is chromatographically pure, the water is deionized water, the ratio of acetonitrile to water is (60% -80%) (20% -40%), isocratic elution is performed, the flow rate is 0.8 mL/min-1.2 mL/min, the sample injection amount of the high performance liquid chromatograph is 8 uL-12 uL, the column temperature of the high performance liquid chromatograph is 20-40 ℃, and the detection wavelength of the high performance liquid chromatograph is 210 nm-230 nm.
(10) Preferably, the ratio of the acetonitrile to the water in the step ③ is 65% to 35%, the flow rate is 1mL/min, the sample amount is 10uL, the chromatographic column is Agilent ZORBAX Eclipse Plus C18, the column temperature is 30 ℃, and the detection wavelength is 220 nm.
(11) Further, in step ④, the cannabidiol and tetrahydrocannabinol standard reference solutions are purchased from Sigma company, usa, and the cannabidiol and tetrahydrocannabinol standard solutions with concentrations of 1mg/mL are respectively diluted with chromatographically pure methanol to be 1ug/mL, 4ug/mL, 10ug/mL, 20ug/mL, 40ug/mL and 100ug/mL of series reference solutions, and are detected by a high performance liquid chromatograph, and a standard curve is drawn by taking the substance concentration as the abscissa and the peak area as the ordinate.
(12) The invention has the advantages that:
① the method uses methanol to carry out ultrasonic extraction of cannabidiol and tetrahydrocannabinol in the cannabis sativa leaves, and has simple experimental operation, short processing time and accurate detection result.
② the invention uses deionized water and acetonitrile as mobile phase, and has good peak shape and separation degree of the target object.
Drawings
Figure 1 cannabidiol standard control chromatogram.
Figure 2 cannabidiol standard curve.
FIG. 3 chromatogram of tetrahydrocannabinol standard control.
Figure 4 tetrahydrocannabinol standard curve.
FIG. 5 example 2 Cannabis mosaic sample chromatogram.
FIG. 6 chromatogram of cannabis mosaic sample in example 3.
FIG. 7 example 4 Cannabis sativa flower and leaf sample chromatogram.
Detailed Description
The following examples are merely illustrative of the present invention and do not limit the scope of the present invention in any way. It will be apparent to those skilled in the art that equivalent embodiments or modifications without departing from the technical spirit of the present invention are within the scope of the present invention.
Example 1
(1) Drying fresh industrial hemp flower and leaf samples at 60 ℃ for 12 hours (the water content is less than 5%), crushing the samples to 20 meshes by a crusher, and uniformly mixing the samples to be tested.
(2) Accurately weighing 0.5g of a flower and leaf sample in a centrifuge tube, adding 10mL of methanol, carrying out ultrasonic extraction for 10 minutes, then centrifuging for 5 minutes by using a centrifuge at 10000 rpm, transferring supernatant into a concentration bottle, carrying out ultrasonic extraction once on residues by using 10mL of methanol repeatedly, centrifuging, combining the supernatants, concentrating by using a rotary evaporator (at the temperature of 40 ℃) to remove part of solvent, transferring concentrated solution into a 10mL volumetric flask, carrying out constant volume on methanol, and passing through a 0.22um organic filter membrane to obtain a solution to be detected.
(3) The instrument conditions of a high performance liquid chromatograph (Agilent 1260) are set as follows: the ratio of mobile phase chromatographic pure acetonitrile to deionized water is 70%: 30 percent, isocratic elution with the flow rate of 0.8 mL/min; the sample size was 10 uL. The chromatographic column is AgilentZORBAX Eclipse Plus C18, the column temperature is 30 ℃; the detection wavelength was 220 nm.
(4) Preparing a series of reference substance solutions: cannabidiol and tetrahydrocannabinol standard solutions are purchased from Sigma company in America, cannabidiol and tetrahydrocannabinol standard solutions with the concentration of 1mg/mL are respectively diluted into series of reference substance solutions with the concentration of 1ug/mL, 4ug/mL, 10ug/mL, 20ug/mL, 40ug/mL and 100ug/mL by chromatographic pure methanol, the high performance liquid chromatograph is used for detection under the chromatographic conditions, and a standard curve is drawn by taking the substance concentration as a horizontal coordinate and taking the peak area as a vertical coordinate.
(5) Quantifying by an external standard method: under the chromatographic conditions, a sample to be detected is detected by liquid chromatography, the corresponding retention time of standard reference substances of cannabidiol and tetrahydrocannabinol is compared, the peak area values of cannabidiol and tetrahydrocannabinol are recorded, the peak areas are brought into an external standard curve, the concentrations of cannabidiol and tetrahydrocannabinol in the liquid to be detected can be obtained, and then the contents of cannabidiol and tetrahydrocannabinol in the hemp flower leaf sample are calculated.
Example 2
(1) Drying fresh industrial hemp flower and leaf samples at 60 ℃ for 12 hours (the water content is less than 5%), crushing the samples to 20 meshes by a crusher, and uniformly mixing the samples to be tested.
(2) Accurately weighing 1.0g of a flower and leaf sample in a centrifuge tube, adding 10mL of methanol, carrying out ultrasonic extraction for 20 minutes, then centrifuging for 5 minutes by using a centrifuge at 10000 rpm, transferring supernatant into a concentration bottle, carrying out ultrasonic extraction on residues once by using 10mL of methanol repeatedly, centrifuging, combining the supernatants, concentrating by using a rotary evaporator (at the temperature of 40 ℃) to remove part of solvent, transferring the concentrated solution into a 10mL volumetric flask, carrying out constant volume on methanol, and passing through a 0.22um organic filter membrane to obtain a solution to be detected.
(3) The instrument conditions of a high performance liquid chromatograph (Agilent 1260) are set as follows: the ratio of mobile phase chromatographically pure acetonitrile to deionized water was 65%: 35 percent, isocratic elution with the flow rate of 1 mL/min; the sample injection amount is 10 uL; the chromatographic column is Agilent ZORBAXeclipse Plus C18, and the column temperature is 30 ℃; the detection wavelength was 220 nm.
(4) The series of control solutions was prepared as in example 1.
(5) External standard method the same as example 1.
Example 3
(1) Drying fresh industrial hemp flower and leaf samples at 60 ℃ for 12 hours (the water content is less than 5%), crushing the samples to 20 meshes by a crusher, and uniformly mixing the samples to be tested.
(2) Accurately weighing 1.0g of a flower and leaf sample in a centrifuge tube, adding 10mL of ethanol, carrying out ultrasonic extraction for 20 minutes, then centrifuging for 5 minutes by using a centrifuge at 10000 rpm, transferring supernatant into a concentration bottle, carrying out ultrasonic extraction on residues once by using 10mL of ethanol, centrifuging, combining the supernatants, concentrating by using a rotary evaporator (at the temperature of 40 ℃) to remove part of solvent, transferring the concentrated solution into a 10mL volumetric flask, carrying out ethanol constant volume, and passing through a 0.22um organic filter membrane to obtain a solution to be detected.
(3) The high performance liquid chromatograph (Agilent 1260) instrument conditions were set up as in example 2.
(4) The series of control solutions was prepared as in example 1.
(5) External standard method the same as example 1.
Example 4
(1) Drying fresh industrial hemp flower and leaf samples at 60 ℃ for 12 hours (the water content is less than 5%), crushing the samples to 20 meshes by a crusher, and uniformly mixing the samples to be tested.
(2) Accurately weighing 1.0g of a flower and leaf sample into a centrifuge tube, adding 10mL of mixed solution of n-hexane and ethyl acetate (the volume ratio is 9: 1), carrying out ultrasonic extraction for 20 minutes, then centrifuging for 5 minutes at 10000 rpm by using a centrifuge, transferring supernatant into a concentration bottle, repeatedly carrying out ultrasonic extraction on residues once by using 10mL of mixed solution of n-hexane and ethyl acetate, centrifuging, combining the supernatant, concentrating by using a rotary evaporator (the temperature is 40 ℃) to remove part of solvent, transferring concentrated solution into a 10mL volumetric flask, fixing the volume of the mixed solution of n-hexane and ethyl acetate, and filtering by using a 0.22um organic filter membrane to obtain a solution to be detected.
(3) The high performance liquid chromatograph (Agilent 1260) instrument conditions were set up as in example 2.
(4) The series of control solutions was prepared as in example 1.
(5) External standard method the same as example 1.
Remarking: as can be seen from fig. 7, tetrahydrocannabinol did not peak at 29.7min with n-hexane-ethyl acetate as the extraction solvent.
Example 5
(1) Drying fresh industrial hemp flower and leaf samples at 60 ℃ for 12 hours (the water content is less than 5%), crushing the samples to 20 meshes by a crusher, and uniformly mixing the samples to be tested.
(2) Accurately weighing 2g of a flower and leaf sample in a centrifuge tube, adding 10mL of methanol, carrying out ultrasonic extraction for 30 minutes, then centrifuging for 5 minutes by using a centrifuge at 10000 rpm, transferring supernatant into a concentration bottle, carrying out ultrasonic extraction on residues once by using 10mL of methanol repeatedly, centrifuging, combining the supernatants, concentrating by using a rotary evaporator (the temperature is 40 ℃) to remove part of solvent, transferring concentrated solution into a 10mL volumetric flask, carrying out constant volume on methanol, and passing through a 0.22um organic filter membrane to obtain a solution to be detected.
(3) The instrument conditions of a high performance liquid chromatograph (Agilent 1260) are set as follows: the ratio of mobile phase chromatographic pure acetonitrile to deionized water is 60%: 40 percent, isocratic elution with the flow rate of 1 mL/min; the sample injection amount is 10 uL; the chromatographic column is Agilent ZorbaxSB-C18, and the column temperature is 30 ℃; the detection wavelength was 220 nm.
(4) The series of control solutions was prepared as in example 1.
(5) External standard method the same as example 1.
Claims (7)
1. A method for measuring the content of cannabidiol and tetrahydrocannabinol in industrial cannabis sativa leaves is characterized by comprising the following steps:
(1) drying a fresh industrial hemp flower and leaf sample in an oven at 60 ℃, crushing by a crusher, and uniformly mixing to be tested;
(2) accurately weighing a flower and leaf sample in a centrifuge tube, adding an organic solvent, carrying out ultrasonic extraction, centrifuging to collect supernatant, repeatedly carrying out ultrasonic extraction on residues once, centrifuging, combining supernatants, and concentrating to a constant volume to obtain a solution to be detected;
(3) detecting a liquid to be detected by using a high performance liquid chromatograph, wherein a mobile phase of the high performance liquid chromatograph is water and acetonitrile, and isocratic elution is carried out;
(4) quantification using external standard: preparing series of reference solutions with cannabidiol and tetrahydrocannabinol standard reference substances, and measuring the content of cannabidiol and tetrahydrocannabinol in the hemp flower and leaf solution by external standard method.
2. The assay method according to claim 1, wherein the hemp flower leaves are a leaf and bud composition of mature industrial hemp picked in 9 months, dried at 60 ℃ until the moisture is less than 5%, and pulverized to 20 mesh by a pulverizer.
3. The determination method according to claim 1, wherein the organic solvent is a chromatographic pure solvent, and is one or a mixture of two of methanol, ethanol, n-hexane and ethyl acetate, and the mass ratio of the volume amount of the organic solvent to the mosaic sample is 5-20: 1.
4. The method according to claim 1, wherein the ultrasonic time is 5 to 30min, the ultrasonic power is 250W, and the operating frequency is 40 kHz.
5. The method according to claim 1, wherein the centrifugation is performed at a rotation speed of 5000 to 10000 rpm for 5 to 10 minutes.
6. The assay method of claim 1, wherein the high performance liquid chromatograph is brand model number agilent 1260; the mobile phase acetonitrile is chromatographically pure, the water is deionized water, and the proportion of the acetonitrile to the water is (60% -80%): (20% -40%), isocratic elution is carried out, and the flow rate is 0.8 mL/min-1.2 mL/min; the sample injection amount of the high performance liquid chromatograph is 8 uL-12 uL; the column temperature of the high performance liquid chromatograph is 20-40 ℃; the detection wavelength of the high performance liquid chromatograph is 210 nm-230 nm.
7. The method as claimed in claim 1, wherein the standard reference solutions of cannabidiol and tetrahydrocannabinol are purchased from Sigma, USA, and prepared into a series of reference solutions, and the standard solutions are detected by HPLC, and a standard curve is drawn by using the substance concentration as abscissa and the peak area as ordinate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911103733.7A CN110780003A (en) | 2019-11-13 | 2019-11-13 | Method for measuring content of cannabidiol and tetrahydrocannabinol in industrial cannabis sativa leaves |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911103733.7A CN110780003A (en) | 2019-11-13 | 2019-11-13 | Method for measuring content of cannabidiol and tetrahydrocannabinol in industrial cannabis sativa leaves |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110780003A true CN110780003A (en) | 2020-02-11 |
Family
ID=69390771
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911103733.7A Withdrawn CN110780003A (en) | 2019-11-13 | 2019-11-13 | Method for measuring content of cannabidiol and tetrahydrocannabinol in industrial cannabis sativa leaves |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110780003A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112730697A (en) * | 2020-09-15 | 2021-04-30 | 中国标准化研究院 | Method for simultaneously detecting 5 cannabinol compounds by using HPLC-MS/MS |
| CN113341008A (en) * | 2021-05-24 | 2021-09-03 | 金华海关综合技术服务中心 | Method and device for detecting cannabidiol and tetrahydrocannabinol in cosmetics |
| CN113340757A (en) * | 2021-05-24 | 2021-09-03 | 金华海关综合技术服务中心 | Method and device for rapidly detecting tetrahydrocannabinol in cosmetics |
| CN114527201A (en) * | 2021-09-01 | 2022-05-24 | 吉林省农业科学院 | Detection method of cannabidiol and tetrahydrocannabinol in cannabis sativa |
| CN115407000A (en) * | 2022-10-17 | 2022-11-29 | 中国计量大学 | Detection method of cannabidiol and tetrahydrocannabinol in industrial cannabis sativa |
| CN115792008A (en) * | 2022-11-29 | 2023-03-14 | 哈尔滨智慧工业大麻产业发展有限公司 | Method for measuring content of cannabidiol in medical-grade high-purity cannabidiol |
-
2019
- 2019-11-13 CN CN201911103733.7A patent/CN110780003A/en not_active Withdrawn
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112730697A (en) * | 2020-09-15 | 2021-04-30 | 中国标准化研究院 | Method for simultaneously detecting 5 cannabinol compounds by using HPLC-MS/MS |
| CN112730696A (en) * | 2020-09-15 | 2021-04-30 | 中国标准化研究院 | Method for detecting 5 cannabinol compounds in cannabis sativa oil by using HPLC (high performance liquid chromatography) method |
| CN113341008A (en) * | 2021-05-24 | 2021-09-03 | 金华海关综合技术服务中心 | Method and device for detecting cannabidiol and tetrahydrocannabinol in cosmetics |
| CN113340757A (en) * | 2021-05-24 | 2021-09-03 | 金华海关综合技术服务中心 | Method and device for rapidly detecting tetrahydrocannabinol in cosmetics |
| CN113340757B (en) * | 2021-05-24 | 2022-12-13 | 金华海关综合技术服务中心 | Quick detection device of tetrahydrocannabinol in cosmetics |
| CN114527201A (en) * | 2021-09-01 | 2022-05-24 | 吉林省农业科学院 | Detection method of cannabidiol and tetrahydrocannabinol in cannabis sativa |
| CN115407000A (en) * | 2022-10-17 | 2022-11-29 | 中国计量大学 | Detection method of cannabidiol and tetrahydrocannabinol in industrial cannabis sativa |
| CN115792008A (en) * | 2022-11-29 | 2023-03-14 | 哈尔滨智慧工业大麻产业发展有限公司 | Method for measuring content of cannabidiol in medical-grade high-purity cannabidiol |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110780003A (en) | Method for measuring content of cannabidiol and tetrahydrocannabinol in industrial cannabis sativa leaves | |
| CN110330409B (en) | Preparation method of industrial hemp extract | |
| CN107337586A (en) | A kind of method of extraction purification cannabidiol in fiber crops from the Chinese | |
| CN110780013A (en) | Method for determining content of Cannabidiol (CBDV) in industrial cannabis sativa leaves | |
| CN104306745A (en) | Quality control method for rhizoma gastrodiae capsule | |
| CN108693257A (en) | The method for directly detecting tealeaves glucosides bound state aroma precursor substance | |
| CN111122744A (en) | Method for detecting content of one or more cannabinoids in industrial hemp extract by using HPLC | |
| CN112034059A (en) | Method for detecting cannabinoids in industrial hemp flowers and leaves and extract thereof by high performance liquid chromatography | |
| Lee et al. | Determination of glucosinolates in traditional Chinese herbs by high-performance liquid chromatography and electrospray ionization mass spectrometry | |
| CN103655844B (en) | The preparation method of Changshan grapefruit peel pomace extract and the preparation containing this extract | |
| CN114010675B (en) | Preparation method and application of Shennong chrysanthemum stem and leaf extract | |
| CN111116323A (en) | Microwave-assisted subcritical technology for extracting cannabidiol and preparation method thereof | |
| CN110092767A (en) | A method of Sync enrichment purification of paclitaxel and 10-DAB III from Chinese yew | |
| CN108299368B (en) | Flavonoid compound and preparation method and application thereof | |
| CN105924420B (en) | The method that Quercetin and phloretin are extracted from Camellia Leaves | |
| CN109884199B (en) | Method for measuring content of flavonoid components in honey | |
| CN110849996A (en) | Method for determining content of cannabichromene (CBC) in industrial cannabis sativa leaves | |
| Charles Dorni et al. | UHPLC–Q-ToF-MS-guided enrichment and purification of triterpenoids from Centella asiatica (L.) extract with macroporous resin | |
| CN110827995B (en) | Characterization method of chromatography and mass spectrum fingerprint spectrum of secondary metabolites of nudiflower purple beautyberry medicinal material | |
| CN107353205A (en) | Ester type compound in nigella glandulifera Freyn seed and its production and use | |
| CN110776541B (en) | Preparation method and application of quercetin-3-gentiobioside | |
| Liu et al. | Phytochemical analysis of Ziziphus jujube leaf at different foliar ages based on widely targeted metabolomics | |
| WO2005087338A1 (en) | Process for extraction of diterpenes and triterpenes from biomaterial | |
| CN107056800A (en) | New component and its separation method and application in a kind of Tsuga longibracteata | |
| CN110954618A (en) | Method for detecting Cannabigerol (CBG) in industrial cannabis sativa by using HPLC |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20200211 |
|
| WW01 | Invention patent application withdrawn after publication |