CN110772548A - Lysimachia capillipes extract and preparation method and application thereof - Google Patents
Lysimachia capillipes extract and preparation method and application thereof Download PDFInfo
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- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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Abstract
The invention discloses a lysimachia capillipes hemsl extract, which contains 10-35% of total flavonoids and 10-65% of total saponins, and is prepared by the steps of crushing lysimachia capillipes hemsl, placing the crushed lysimachia capillipes hemsl in a container, adding 50-95% ethanol in an amount which is 6-12 times that of the crushed lysimachia capillipes hemsl, and performing ultrasonic extraction for 15-60 minutes or percolation to obtain an ethanol extract; concentrating the obtained ethanol extract to obtain an extract without alcohol smell, diluting with 3-8 times of water to obtain a diluent, standing, centrifuging, adding n-butanol into the centrifugate according to the volume ratio of 1:1 for extraction, separating to obtain n-butanol extract, recovering the solvent from the n-butanol extract, and drying to obtain the lysimachia capillipes hemsl extract. The lysimachia capillipes hemsl extract has relatively clear components, is suitable for industrial production, has high extraction rate and high extraction speed, and has pharmacological activity of analgesia and anti-inflammation.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicine pharmacy, and particularly relates to a lysimachia capillipes hemsl extract, a preparation method thereof and application thereof in preparing analgesic and anti-inflammatory medicines.
Background
Lysimachia capillipes (scientific name: Lysimachia capillipes) is a plant of the genus Phyllanthus of the family Primulaceae. The full-grass of Manshan mountain incense, lysimachia foenum-graecum, lysimachia sikokiana, vanilla and phyllanthus urinaria, has sweet and mild properties, is used as a medicine, has the effects of dispelling wind, relieving cough, regulating menstruation and the like, and is used for treating cold, cough, bronchitis, asthma, irregular menstruation, neurasthenia and the like. The lysimachia capillipes hemsl medicinal material mainly contains flavonoid, saponins, lactones, acids and other components. The single medicinal material preparation prepared from the flavonoid components is used for treating cold; the saponin is another main component of the lysimachia capillipes and can effectively inhibit the proliferation of various cancer cells and show strong antitumor activity. The researches show that the lysimachia capillipes has a plurality of other functions, so that the lysimachia capillipes extract, the preparation method thereof and the application thereof in preparing analgesic and anti-inflammatory medicines are provided.
Disclosure of Invention
The invention aims to provide a lysimachia capillipes extract and a preparation method and application thereof.
The invention has a technical scheme that:
a Lysimachia capillipes extract contains 10-35% of total flavonoids and 10-65% of total saponins.
The other technical scheme of the invention is as follows:
a method for preparing herba Lysimachiae Capillipedis extract comprises:
(1) crushing lysimachia capillipes into powder of 5-30 meshes, placing the powder in a container, adding ethanol, and performing ultrasonic extraction or percolation at 20-100 KHz to obtain an ethanol extracting solution;
(2) concentrating the ethanol extract to obtain an extract without alcohol smell, diluting the extract with water to obtain a diluent, standing and centrifuging to obtain a centrifugal liquid, adding n-butyl alcohol into the centrifugal liquid for extraction, separating to obtain n-butyl alcohol extract and residual liquid, recovering solvent n-butyl alcohol from the n-butyl alcohol extract to obtain an extract, and drying the extract to obtain the lysimachia capillipes hemsl extract.
Further, in the step (1), the amount of the ethanol is 6-12 times of the weight of the lysimachia capillipes coarse powder.
Further, in the step (1), the concentration of the ethanol is 50-95% by volume.
Further, in the step (1), the extraction time is 15-60 minutes.
Further, in the step (2), the amount of the water is 3-8 times of the weight of the extract.
Further, in the step (2), the rotation speed of the centrifugation is 3000-20000 revolutions per minute.
Further, in the step (2), the volume ratio of the centrifugate to the n-butanol is 1: 1.
The lysimachia capillipes hemsl extract prepared in the mode is applied to anti-inflammatory analgesic drugs, and the anti-inflammatory analgesic drugs are oral preparations or external preparations.
The invention provides a lysimachia capillipes extract and a preparation method thereof, and the main advantages are that: the process is simple, convenient and practical; the Lysimachia capillipes extract has analgesic and anti-inflammatory effects, and expands the application value of the Lysimachia capillipes.
Detailed Description
The lysimachia capillipes hemsl extract contains 10-35% of total flavonoids and 10-65% of total saponins.
The preparation method comprises the following steps:
crushing lysimachia capillipes into 5-30 meshes of powder, placing the powder into a container, adding 6-12 times of 50-95% ethanol, and carrying out ultrasonic extraction for 15-60 minutes at 20-100 KHz or percolation to obtain an ethanol extract;
concentrating the ethanol extract to obtain an extract without alcohol smell, diluting with 3-8 times of water to obtain a diluent, standing, centrifuging at 3000-20000 r/min, adding n-butanol into the centrifugate according to the volume ratio of 1:1 for extraction, separating to obtain n-butanol extract and residual liquid, recovering the solvent from the n-butanol extract, and drying to obtain the lysimachia capillipes hemsl extract.
The obtained herba Lysimachiae Foenumgraeci extract can be used in preparing analgesic and anti-inflammatory drugs.
The present invention also provides a formulation prepared with the Lysimachia capillipes extract and a pharmaceutically acceptable carrier or excipient. The following formulations were selected for these formulations: solutions, suspensions, emulsions, tablets, capsules, granules, pills, lozenges, oral liquids, sublingual tablets, effervescent tablets, patches, ointments and the like.
The pharmaceutical preparation containing the lysimachia capillipes hemsl extract can also optionally contain pharmaceutically acceptable carriers during the preparation of the pharmaceutical preparation, wherein the carriers are from the following components: cosolvent, solubilizer, suspending agent, emulsifier, surfactant, correctant, etc.
In order to make the aforementioned objects, features and advantages of the present invention more comprehensible, embodiments accompanying the present invention are further described below. The invention is not limited to the embodiments listed but also comprises any other known variations within the scope of the invention as claimed.
First, reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one implementation of the invention. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.
Example 1
A method for preparing herba Lysimachiae Capillipedis extract comprises:
(1) taking 5kg of lysimachia capillipes and crushing into powder of 10 meshes; adding 9 times of 65% ethanol, performing ultrasonic extraction for 25 minutes, and filtering to obtain ethanol extract;
(2) and (3) distilling the ethanol extract under reduced pressure, concentrating until no alcohol smell exists to obtain an extract, diluting with 6 times of water to obtain a diluent, standing, centrifuging at 6000r/min, adding n-butanol into the centrifuged clear liquid in a volume ratio of 1:1 for extraction, separating to obtain an n-butanol extract, recovering the n-butanol, and drying the extract to obtain 308g of the lysimachia capillipes hemsl extract.
Example 2
A method for preparing herba Lysimachiae Capillipedis extract comprises:
(1) taking 5kg of lysimachia capillipes and crushing into 5-mesh powder; percolating with 6 times of 75% ethanol, and filtering the percolate to obtain ethanol extractive solution;
(2) distilling the ethanol extract under reduced pressure, concentrating until no alcohol smell exists to obtain an extract, diluting with 5 times of water to obtain a diluent, standing, centrifuging at 20000r/min, adding n-butanol with the volume ratio of 1:1 into the centrifuged clear liquid for extraction, separating to obtain an n-butanol extract, recovering the n-butanol, drying the extract to obtain 334g of the lysimachia capillipes hemsl extract.
Example 3
A method for preparing herba Lysimachiae Capillipedis extract comprises:
(1) taking 5kg of lysimachia capillipes and crushing into 15-mesh powder; adding 10 times of 90% ethanol, performing ultrasonic extraction for 45 minutes, and filtering to obtain ethanol extract;
(2) and distilling the ethanol extract under reduced pressure, concentrating until no alcohol smell exists to obtain an extract, diluting with 8 times of water to obtain a diluent, standing, centrifuging at 15000r/min, adding n-butanol with the volume ratio of 1:1 into the centrifuged clear liquid, extracting twice to obtain an n-butanol extract, recovering the n-butanol, and drying the extract to obtain 312g of the lysimachia capillipes hemsl extract.
Example 4
A method for preparing herba Lysimachiae Capillipedis extract comprises:
(1) taking 5kg of lysimachia capillipes and crushing into powder of 30 meshes; percolating with 12 times of 50% ethanol, and filtering to obtain ethanol extractive solution;
(2) distilling the ethanol extract under reduced pressure, concentrating until no alcohol smell exists to obtain an extract, diluting with 7 times of water to obtain a diluent, standing, centrifuging at 10000r/min, adding n-butanol with the volume ratio of 1:1 into the centrifuged clear liquid, extracting for three times to obtain an n-butanol extract, recovering the n-butanol, and drying the extract to obtain 348g of the lysimachia capillipes hemsl extract.
The contents of total flavonoids and total saponins in the lysimachia capillipes hemsl extracts in examples 1-4 were determined by a colorimetric method, and were respectively: example 1 (total flavonoids 15.6%; total saponins 62.3%), example 2 (total flavonoids 26.3%; total saponins 36.9%), example 3 (total flavonoids 34.5%; total saponins 23.3%) and example 4 (total flavonoids 11.7%; total saponins 18.6%).
Example 5
Pharmacological Activity test
1. Experimental study for inhibiting carrageenan-induced foot swelling in rats
60 healthy SD rats, half male and female, weighing 180-.
Feeding conditions of animals: the air ventilation environment is clean, the humidity is 50 +/-5%, the room temperature is 23 +/-1 ℃, the illumination period is 12 hours, and the water and food are taken by oneself.
The rats were continuously administered by gavage for 4 days, 1 time per day, and fasted for 12h before the experiment. The volume before inflammation was measured by a rat foot volume measuring instrument before the last administration, and 30min after the last administration, 0.1ml of carrageenan was subcutaneously injected into the right hind foot sole of each group of rats to cause inflammation, and the volume after the right hind foot toe inflammation was measured by a foot volume measuring instrument 3h after the inflammation to calculate the swelling degree and the inhibition rate, respectively, and the results are shown in table 1.
Swelling degree (ml) is the volume value of postinflammatory foot-volume value of pro-inflammatory foot
The inhibition ratio (%) (average swelling degree of blank group-average swelling degree of administration group)/average swelling degree of blank group × 100%
TABLE 1 inhibition of carrageenan-induced foot swelling in rats
Comparison of P <0.01 with saline group
2. Hot plate method analgesic experimental study
The water bath is filled with water, the water surface is contacted with the hot plate, the thermostat is adjusted, the water temperature is controlled at 55 +/-5 ℃, and the hot plate needs to be preheated for 10min at the room temperature of 20 +/-1 ℃. 70 Kunming mice with the weight of 18-22 g are taken, females are placed on a hot plate one at a time, and the time (seconds) required for the mice to lick the hind feet or jump off the hot plate from the mice placed on the hot plate is used as the pain threshold value of the mice. The patient does not use the traditional Chinese medicine when the foot licking time is less than 5 seconds or more than 30 seconds. Eligible mice were randomly divided into 6 groups, namely, a physiological saline group (0.9% physiological saline, 10ml/kg), an example 1 group (Lysimachia capillipes extract 40mg/kg,10ml/kg), an example 2 group (Lysimachia capillipes extract 40mg/kg,10ml/kg), an example 3 group (Lysimachia capillipes extract 40mg/kg,10ml/kg), an example 4 group (Lysimachia capillipes extract 40mg/kg,10ml/kg) and a positive control group (aspirin, 130mg/kg,10 ml/kg).
Feeding conditions of animals: the air ventilation environment is clean, the humidity is 50 +/-5%, the room temperature is 23 +/-1 ℃, the illumination period is 12 hours, and the water and food are taken by oneself.
The continuous gavage administration is carried out for 3 days, 1 time is carried out every day, and the fasting is not forbidden for 12 hours before the experiment. After the last administration, the pain threshold of each mouse was determined within 30min, and if the mouse had no pain response for 60s on a hot plate, the mouse was immediately taken out as 60s, and the results are shown in Table 2.
TABLE 2 comparison of pain threshold values in hot plate pain test of groups of mice
Comparison of P <0.01 with saline group
3 acetic acid writhing method analgesia experimental research
90 NIH female mice were selected, weighing about 20g, and randomly divided into 6 groups, each of which 15 groups were a physiological saline group (0.9% physiological saline, 10ml/kg), example 1 group (Lysimachia capillipes extract 40mg/kg,10ml/kg), example 2 group (Lysimachia capillipes extract 40mg/kg,10ml/kg), example 3 group (Lysimachia capillipes extract 40mg/kg,10ml/kg), example 4 group (Lysimachia capillipes extract 40mg/kg,10ml/kg) and a positive control group (aspirin 130mg/kg,10 ml/kg).
Feeding conditions of animals: the air ventilation environment is clean, the humidity is 50 +/-5%, the room temperature is 23 +/-1 ℃, the illumination period is 12 hours, and the water and food are taken by oneself.
Continuous gavage was performed for 3 days, 1 time per day, and mice were fasted for 12h before the experiment. 30min after the last administration, 0.7% glacial acetic acid 10ml/kg is injected into the abdominal cavity, and the times of writhing occurring within 15min are observed. The writhing response is manifested by contraction of the abdomen, inward concavity, body distortion, and extension of the hind limbs. The percentage of analgesic inhibition of each drug was calculated by comparing each drug group with the saline group, and the analgesic effect of the drug was observed, and the results are shown in table 3.
The inhibition rate was calculated according to the following formula:
inhibition rate ═ average number of blanks-average number of treatments)/average number of blanks × 100%.
TABLE 3 Effect on writhing frequency after intraperitoneal acetic acid injection in mice
P <0.05 compared to saline group; comparison of P <0.01 with saline group
Compared with the prior art, the invention has the beneficial effects that: the lysimachia capillipes hemsl extract has relatively clear components, is suitable for industrial production, has high extraction rate and high extraction speed, and has pharmacological activity of analgesia and anti-inflammation. It should be noted that the above-mentioned embodiments are only for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, which should be covered by the claims of the present invention.
Claims (10)
1. A Lysimachia capillipes extract characterized in that: the lysimachia capillipes hemsl extract contains 10-35% by weight of total flavonoids and 10-65% by weight of total saponins.
2. A method for preparing a lysimachia capillipes hemsl extract is characterized by comprising the following steps:
(1) crushing lysimachia capillipes into powder of 5-30 meshes, placing the powder in a container, adding ethanol, and performing ultrasonic extraction or percolation at 20-100 KHz to obtain an ethanol extracting solution;
(2) concentrating the ethanol extract to obtain an extract without alcohol smell, diluting the extract with water to obtain a diluent, standing, centrifuging to obtain a centrifugate, adding n-butanol into the centrifugate for extraction, separating to obtain n-butanol extract and a residual solution, recovering the solvent n-butanol from the n-butanol extract to obtain an extract, and drying the extract to obtain the lysimachia capillipes hemsl extract.
3. The method for preparing a lysimachia capillipes extract according to claim 2, wherein: in the step (1), the amount of the ethanol is 6-12 times of the weight of the lysimachia capillipes coarse powder.
4. The method for preparing a lysimachia capillipes extract according to claim 2, wherein: in the step (1), the concentration of the ethanol is 50-95% by volume.
5. The method for preparing a lysimachia capillipes extract according to claim 2, wherein: in the step (1), the extraction time is 15-60 minutes.
6. The method for preparing a lysimachia capillipes extract according to claim 2, wherein: in the step (2), the amount of the water is 3-8 times of the weight of the extract.
7. The method for preparing a lysimachia capillipes extract according to claim 2, wherein: in the step (2), the rotating speed of the centrifugation is 3000-20000 revolutions per minute.
8. The method for preparing a lysimachia capillipes extract according to claim 2, wherein: in the step (2), the volume ratio of the centrifugate to the n-butanol is 1: 1.
9. Application of herba Lysimachiae Capillipedis extract in anti-inflammatory and analgesic medicine is provided.
10. The use of a lysimachia capillipes extract according to claim 9 in an anti-inflammatory analgesic drug, characterized in that: the anti-inflammatory analgesic medicine is an oral preparation or an external preparation.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111184751A (en) * | 2020-02-19 | 2020-05-22 | 常熟求是科技有限公司 | External preparation containing lysimachia capillipes and application thereof |
| CN111254013A (en) * | 2020-02-21 | 2020-06-09 | 福建中烟工业有限责任公司 | Perfume composition containing Lysimachia capillipes extract |
| CN114264763A (en) * | 2021-12-22 | 2022-04-01 | 金绿源(中国)生物科技有限公司 | Method for sampling lysimachia capillipes |
| CN116115657A (en) * | 2023-03-10 | 2023-05-16 | 中国科学院基础医学与肿瘤研究所(筹) | Use of lysimachia capillipes or extracts thereof as medicines |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102180938A (en) * | 2011-03-30 | 2011-09-14 | 浙江大学 | Method for preparing capilliposide |
| CN102228488A (en) * | 2011-06-07 | 2011-11-02 | 南京泽朗农业发展有限公司 | Preparation of Lysimachia capillipes Hemsl total saponin |
| CN104817601A (en) * | 2015-03-31 | 2015-08-05 | 浙江大学 | Mixture containing three flavone components extracted from Lysimachia capillipes, preparation thereof, and preparation method of mixture and preparation |
| CN109512901A (en) * | 2018-12-31 | 2019-03-26 | 广东罗浮山国药股份有限公司 | Radix Wikstroemae extract and its preparing the application in antalgic and inflammation relieving drug |
-
2019
- 2019-11-13 CN CN201911104724.XA patent/CN110772548A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102180938A (en) * | 2011-03-30 | 2011-09-14 | 浙江大学 | Method for preparing capilliposide |
| CN102228488A (en) * | 2011-06-07 | 2011-11-02 | 南京泽朗农业发展有限公司 | Preparation of Lysimachia capillipes Hemsl total saponin |
| CN104817601A (en) * | 2015-03-31 | 2015-08-05 | 浙江大学 | Mixture containing three flavone components extracted from Lysimachia capillipes, preparation thereof, and preparation method of mixture and preparation |
| CN109512901A (en) * | 2018-12-31 | 2019-03-26 | 广东罗浮山国药股份有限公司 | Radix Wikstroemae extract and its preparing the application in antalgic and inflammation relieving drug |
Non-Patent Citations (5)
| Title |
|---|
| XU ZHANG ET AL.: "In Vitro and In Situ Characterization of the Intestinal Absorption of Capilliposide B and Capilliposide C from Lysimachia capillipes Hemsl", 《MOLECULES》 * |
| 朱兆云: "《云南天然药物图鉴 第7卷》", 29 February 2012, 云南科技出版社 * |
| 杨芳等: ""排草香细胞毒性及木犀草素含量的测定"", 《实用药物与临床》 * |
| 梁敬钰: "《天然药物化学实验与指导》", 30 September 2010, 中国医药科技出版社 * |
| 赵长祺等: ""木犀草素的药理作用研究进展"", 《承德医学院学报》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111184751A (en) * | 2020-02-19 | 2020-05-22 | 常熟求是科技有限公司 | External preparation containing lysimachia capillipes and application thereof |
| CN111254013A (en) * | 2020-02-21 | 2020-06-09 | 福建中烟工业有限责任公司 | Perfume composition containing Lysimachia capillipes extract |
| CN111254013B (en) * | 2020-02-21 | 2023-07-21 | 福建中烟工业有限责任公司 | Perfume composition containing extract of lysimachia capillipes |
| CN114264763A (en) * | 2021-12-22 | 2022-04-01 | 金绿源(中国)生物科技有限公司 | Method for sampling lysimachia capillipes |
| CN116115657A (en) * | 2023-03-10 | 2023-05-16 | 中国科学院基础医学与肿瘤研究所(筹) | Use of lysimachia capillipes or extracts thereof as medicines |
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Application publication date: 20200211 |