CN110763782B - Content determination method of Qianlieping capsules - Google Patents
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Abstract
The invention relates to a method for measuring the content of a Qianlieping capsule, which adopts liquid chromatography to measure the content of luteolin in a Qianlieping preparation, and increases the high performance liquid chromatography content measurement of luteolin in the Junyao-Baijiang on the basis of the original standard. The invention has strong specificity for detecting the content of the patrinia scabiosaefolia fisch.f, is simple and easy to operate, is rapid and reliable, and can effectively control the quality of the Qianlieping capsule, so that the quality of the Qianlieping capsule is stable and controllable, the curative effect is ensured, and the medical requirement is better met.
Description
Technical Field
The invention belongs to the technical field of pharmacy, and particularly relates to a content determination method of Qianlieping capsules for treating acute and chronic prostatitis.
Background
Prostatitis is a common disease of men, most of which occurs in young and strong years, and can be divided into acute and chronic prostatitis clinically. Acute prostatitis is less common clinically, chronic prostatitis has higher morbidity in adult people, accounts for about 1/5 of urologic outpatients, and chronic prostatitis is also called as prostatic seminal vesiculitis because the chronic prostatitis is accompanied with the seminal vesiculitis.
Qianlieping capsule, standard WS-10459(ZD-0459) -2012Z-2018, whose prescription is 1000 granules prepared from herba patriniae 702g, root of red rooted saliva 234g, red peony root 234g, peach kernel 234g, safflower 234g, eupatorium japonicum 234g, pyrrosia leaf 234g, frankincense 47g, myrrh 47 g. It has effects of clearing heat, promoting diuresis, removing blood stasis and relieving pain, and can be used for treating acute and chronic prostatitis caused by stagnation of damp-heat. Chinese patent 200510096360.7 discloses a capsule for treating acute and chronic prostatitis and its preparation method. The capsule is prepared from nine traditional Chinese medicinal materials including patrinia scabiosaefolia, salvia miltiorrhiza, red paeony root, peach kernel, safflower, herba lycopi, pyrrosia lingua, frankincense and myrrh, has a synergistic effect, accords with the principle of a traditional Chinese medicine formula, and can achieve the purpose of treating both principal and secondary aspects of diseases. In the formula, the patrinia scabiosaefolia fisch has the functions of strongly clearing heat and removing toxicity, promoting blood circulation and expelling pus, and relieving swelling and itching, and the peach kernel and the safflower have strong functions of promoting blood circulation, removing blood stasis, dispelling distension and relieving pain, and are used as monarch drugs together; herba lycopi, pyrrosia lingua and talc help monarch drugs to clear heat and promote diuresis, and promote diuresis and treat stranguria are ministerial drugs; the salvia miltiorrhiza, the red paeony root, the frankincense and the myrrh are used as adjuvant drugs for activating blood circulation, removing blood stasis, promoting qi circulation and relieving pain. The medicines have the functions of clearing heat, promoting diuresis, removing blood stasis and relieving pain, so that the inflammation of the prostatic hyperplasia can be reduced, and the pain of urine can be relieved. The clinical application process of hospitals all over the country produced by the Xian Qian He pharmacy Limited company proves that the medicine has obvious clinical curative effect and is deeply trusted by wide medical workers and patients.
In the existing detection methods of the medicines for treating acute and chronic prostatitis, such as the prostate capsule, only the content detection of the salvia miltiorrhiza and the red paeony root is carried out by a High Performance Liquid Chromatography (HPLC), and the content detection method of other components, particularly the north patrinia scabiosaefolia link, is not established. The quality and the curative effect of the patrinia scabiosaefolia fisch are greatly influenced by being taken as an important raw material medicine. In order to further control the quality of the product and ensure the curative effect, the detection method of the product needs to be perfected. The test items of the herba patriniae medicinal material include caffeic acid and luteolin. Caffeic acid is stable in property, luteolin is a natural flavonoid compound, exists in various plants, and has various pharmacological activities such as anti-inflammation and anti-allergy, and the anti-inflammatory activity of luteolin is considered to be related to the inhibition of the generation of Nitric Oxide (NO) and other inflammatory cytokines such as tumor necrosis factor-alpha (TNF-alpha), leukocyte mediated-6 (IL-6), the inhibition of the phosphorylation of protein tyrosine and the gene expression mediated by nuclear transcription factor KB (NF-KB). In conclusion, the quality of the medicine is more effectively controlled and is stable and controllable by researching the detection method of the luteolin content of the patrinia scabiosaefolia link in the Qianliping capsule, so that the medical needs are better met.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a detection method for determining luteolin in a Qianlieping capsule, and the detection method can effectively detect the content of active ingredients of a medicament, thereby controlling the quality of a product, ensuring the curative effect and better meeting the medical needs.
The purpose of the invention is realized by adopting the following technical scheme:
a method for measuring the content of a Qianlieping capsule adopts liquid chromatography to measure the content of luteolin in North Patrinia, and specifically comprises the following steps:
(1) conditions of liquid chromatography
Octadecylsilane chemically bonded silica gel column is used as a filling agent; the volume ratio is 20: 80-40: 60 acetonitrile-1.0% formic acid solution is used as mobile phase; an ultraviolet detector with the detection wavelength of 250-360 nm; the flow rate is 0.6ml/min-1.2 ml/min; the column temperature is 25-45 ℃; the number of theoretical plates is not less than 4000 calculated according to luteolin peak;
(2) preparation of control solutions
Accurately weighing appropriate amount of luteolin control, drying in phosphorus pentoxide desiccator for 24 hr, adding methanol to obtain solution containing 10 μ g per lml;
(3) preparation of test solution
Precisely weighing 0.8-1.5g of sample under the prostate capsule loading term, Soxhlet extracting with 50-300ml of methanol for 1-3h, filtering, washing residue, mixing filtrates, evaporating to dryness, precisely adding 25ml of methanol, shaking up, taking 10ml of subsequent filtrate, adding 10ml of acetone, standing to obtain supernatant, and filtering with 0.45 μm microporous membrane to obtain sample solution;
(4) measurement of
Precisely sucking 10-20 μ l of each of the reference solution and the sample solution, respectively, injecting into high performance liquid chromatograph, and measuring to obtain measurement result.
Preferably, the volume ratio is 30:70 acetonitrile-1.0% formic acid solution as mobile phase.
Preferably, the detection wavelength is 350 +/-2 nm; the flow rate is 1.0 ml/min; the column temperature was 35 ℃.
Preferably, the preparation method of the test solution is as follows: precisely weighing 1.2g of a sample under the prostate capsule loading term, performing Soxhlet extraction with 200ml of methanol for 2h, filtering, washing residues, combining filtrates, evaporating to dryness, precisely adding 25ml of methanol, shaking uniformly, taking 10ml of subsequent filtrate, adding 10ml of acetone, standing to obtain a supernatant, and filtering with a 0.45 mu m microporous filter membrane to obtain a sample solution.
Compared with the prior art, the invention has the following beneficial effects:
the invention adds the high performance liquid chromatography content determination of luteolin in the monarch drug, namely, North Patrinia, on the basis of the original standard, and the methodology investigation proves that the method has good precision and accuracy, no interference in negative, strong operability in the whole test process, and can be used as one of indexes for controlling the internal quality of the product. The invention has strong specificity for detecting the content of the patrinia scabiosaefolia fisch.f, is simple and easy to operate, is rapid and reliable, and can effectively control the quality of the Qianlieping capsule, so that the quality of the Qianlieping capsule is stable and controllable, the curative effect is ensured, and the medical requirement is better met.
Drawings
FIG. 1 is a chromatogram of the first luteolin content determination method of the invention;
FIG. 2 is a chromatogram of a third method for determining luteolin content of the present invention;
FIG. 3 is a chromatogram of a fourth method for determining luteolin content of the present invention;
FIG. 4 is a chromatogram for the specific study of the fourth method for determining luteolin content of the present invention; in the figure, a, b, c and d are chromatograms of a reference solution, a test solution, a negative test solution and a blank solution respectively;
FIG. 5 is a linear relationship diagram of the fourth method for determining luteolin content of the present invention.
Detailed Description
In order that the above objects, features and advantages of the present invention can be more clearly understood, a detailed description of the present invention will be given below with reference to the accompanying drawings and specific embodiments. In the following description, numerous specific details are set forth to provide a thorough understanding of the present invention, and the described embodiments are merely a subset of the embodiments of the present invention, rather than a complete embodiment. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows: prescription of Qianlieping capsule and preparation method
Prescription:
702g of herba patriniae, 234g of salvia miltiorrhiza, 234g of red peony root, 234g of peach kernel, 234g of safflower
Herba Lycopi 234g folium Pyrrosiae 234g Olibanum 47g Myrrha 47g
The preparation method comprises the following steps: the above nine ingredients, frankincense and myrrh are smashed into fine powder for standby. Pulverizing the red sage root and the red paeony root into coarse powder, adding ethanol for extraction twice, extracting for 2 hours each time, combining extracting solutions, filtering, recovering ethanol from filtrate, and concentrating to obtain thick paste with the relative density of 1.32-1.36 (60 ℃) for later use. Decocting the rest five ingredients such as the patrinia scabiosaefolia link and the dregs of the decoction after alcohol extraction twice with water for 2 hours for the first time and 1.5 hours for the second time, merging the decoction, filtering, concentrating the filtrate under reduced pressure to obtain thick paste with the relative density of 1.32-1.36 (60 ℃), adding the fine powder and the thick paste, mixing uniformly, adding an appropriate amount of auxiliary materials, preparing into granules, drying, and encapsulating to obtain 1000 granules.
The characteristics are as follows: the product is capsule, and the content is brown to brown granules and powder; fragrant, bitter and slightly sour.
Example two: content identification of luteolin in patrinia scabiosaefolia link capsule
1. Optimization test of mobile phase and test article preparation method
Determining the content of luteolin as an effective ingredient of herba Patriniae in the Qianliping capsule by adopting an HPLC method, wherein the chromatographic conditions are as follows:
filling agent: octadecylsilane chemically bonded silica; column temperature: 35 ℃; an ultraviolet detector is adopted, and the detection wavelength is preferably 348 nm; the number of theoretical plates should be not less than 4000, calculated from the luteolin peak. The flow rate was 1 ml/min.
Mobile phase optimization selection comparison:
mobile phase 1: the mobile phase is a methanol-water solution with the volume ratio of 30: 70.
Mobile phase 2: the mobile phase is 30:70 acetonitrile-1.0% (v/v) formic acid solution by volume.
The following sample treatment methods were combined for the experiments:
the invention relates to a method for detecting the content of luteolin in a north patrinia herb in a Qianlieping capsule by high performance liquid chromatography, which comprises the following steps:
1.1 preparation of control solutions
Accurately weighing appropriate amount of luteolin control, drying in phosphorus pentoxide desiccator for 24 hr, and adding methanol to obtain solution containing 10 μ g per lml.
1.2 preparation of test solutions
Precisely weighing 1.2g of a sample under the item of prostate capsule loading, and processing the sample by the following method for a comparison test. 1.2.1 sample treatment methods: adding methanol 25ml precisely, ultrasonic treating for 20min, shaking and filtering to obtain the final product.
1.2.2 sample treatment methods: soxhlet extracting with 200ml methanol for 2h, filtering, washing residue with appropriate amount of methanol, mixing filtrates, evaporating to dryness, adding methanol 25ml precisely, and shaking.
1.2.3 sample treatment methods: soxhlet extracting with methanol 200ml for 2h, filtering, washing residue with appropriate amount of methanol, mixing filtrates, evaporating to dryness, adding methanol 25ml precisely, shaking, collecting filtrate 10ml, adding acetone 10ml, and standing supernatant as sample solution.
1.3 determination of
Precisely sucking 10 μ l of each of the reference solution and the sample solution, injecting into a liquid chromatograph, and measuring;
the method comprises the following steps: the mobile phase 1 and the sample processing method 1.2.1 are selected, and the rest are unchanged, so that the result is shown in figure 1, the separation effect of the sample is poor, and the effective separation of the contents in the sample cannot be proved.
The second method comprises the following steps: the mobile phase 1 and the sample treatment method 1.2.2 are selected for detection, and the result is improved compared with the method for detecting the peak shape, but the improvement is not obvious, and the separation degree of the luteolin sample peak is not good.
The third method comprises the following steps: the results of the mobile phase 1 and the sample processing method 1.2.3 are shown in FIG. 2. The peak shape presents normal distribution, the separation effect is better than that of the method I and the method II, the selection of the mobile phase is judged to have certain influence possibly, and the sample treatment method 1.2.3 is the best sample treatment method.
The method four comprises the following steps: the mobile phase 2 and the sample processing method 1.2.3 are selected, the peak shape is normal, and the required separation degree can be achieved, and a large number of experiments show that the acetone solvent is added into the test sample solution under the condition of using the mobile phase 2, so that the unexpected technical effects are achieved: the peak shape of the luteolin sample peak is remarkably improved, and the separation degree is remarkably improved. Indicating the unexpected improvement of the peak shape by acetone and the ability to increase the separation between two adjacent peaks.
Through research and experiments on the mobile phase and the sample processing method, an optimized scheme can be selected, the method IV is the currently optimal method, and the feasibility of the method can be further confirmed through the following method research.
2. Research on specificity:
2.1 chromatographic conditions and System suitability test
Octadecylsilane chemically bonded silica is used as a filling agent; acetonitrile-1.0% (v/v) formic acid solution (30: 70) is used as a mobile phase; the detection wavelength was 348 nm. The number of theoretical plates should be not less than 4000, calculated from the luteolin peak.
Preparation of control solutions:
accurately weighing appropriate amount of luteolin control, drying in phosphorus pentoxide desiccator for 24 hr, and adding methanol to obtain solution containing 10 μ g per lml.
Preparation of a test solution:
precisely weighing a sample under the prostate capsule loading term, performing Soxhlet extraction for 2h by using methanol, evaporating to dryness, precisely adding 25ml of methanol, shaking uniformly, continuously taking 10ml of filtrate, adding 10ml of acetone, and standing supernatant to be used as a test sample solution.
And (3) determination: precisely sucking 10 μ l of each of the reference solution, the negative sample solution and the sample solution, injecting into a liquid chromatograph, and measuring;
the results show that: in the chromatogram of the test solution, the same chromatographic peak is arranged at the corresponding position of the chromatographic peak of the luteolin reference substance; negative and blank reagents are not interfered.
2.2 the concentration of the luteolin control prepared according to the requirements of item 1.1 is 11.35 μ g/ml
2.3 preparation of test solution:
1.2001g of prostate capsule under the item of the packaging amount of prostate capsule was precisely weighed, and the sample was processed according to the method under the item 1.2.3 of the sample processing method.
2.4 negative test article solution:
weighing other raw materials and adjuvants except herba Patriniae according to the proportion of the formula, making into herba Patriniae negative sample, precisely weighing 1.2011g, and preparing according to the preparation method of test solution under item 1.2.3.
The negative sample prescription and the preparation method are as follows:
salvia miltiorrhiza 234g red peony root 234g peach kernel 234g lycopus 234g
Pyrrosia leaf 234g frankincense 47g myrrh 47g safflower 234g
The preparation method comprises the following steps: pulverizing Olibanum and Myrrha into fine powder. Pulverizing the red sage root and the red paeony root into coarse powder, adding ethanol for extraction twice, extracting for 2 hours each time, combining extracting solutions, filtering, recovering ethanol from filtrate, and concentrating to obtain thick paste with the relative density of 1.32-1.36 (60 ℃) for later use. Decocting the rest four ingredients such as the patrinia scabiosaefolia and the dregs of the decoction after alcohol extraction twice with water for 2 hours for the first time and 1.5 hours for the second time, merging the decoction, filtering, concentrating the filtrate under reduced pressure to form thick paste with the relative density of 1.32-1.36 (60 ℃), adding the fine powder and the thick paste, mixing uniformly, drying, and grinding into fine powder to obtain the traditional Chinese medicine.
2.5 preparation of blank solution: methanol was taken directly as a blank solution. And recording the chromatogram.
2.6 injecting 10 mul of the sample respectively, and the detailed analysis map is shown in figure 4.
2.7 results: in the chromatogram of the test solution, the same chromatographic peak is arranged at the corresponding position of the chromatographic peak of the luteolin reference substance; negative and blank reagents are not interfered.
2.8 conclusion: the specificity is good.
3. Precision of the instrument:
3.1 chromatographic conditions and System suitability test
Octadecylsilane chemically bonded silica is used as a filling agent; in acetonitrile-1.0% (v/v) formic acid solution (30: 70); the detection wavelength was 348 nm. The number of theoretical plates should be not less than 4000, calculated from the luteolin peak.
3.2 taking luteolin reference substance (concentration is 11.35 μ g/ml) solution, precisely measuring 10 μ l, injecting into liquid chromatograph, repeating sample injection for 6 times, and calculating peak area RSD. The results are shown in Table 1.
TABLE 1 precision test data sheet
3.3 the results show that the RSD value of the peak area is 0.3%, less than 2.0%.
3.4 conclusion: the precision of the instrument is good.
4. Linearity:
4.1 chromatographic conditions and System suitability test
Octadecylsilane chemically bonded silica is used as a filling agent; in acetonitrile-1.0% (v/v) formic acid solution (30: 70); the detection wavelength was 348 nm. The number of theoretical plates should be not less than 4000, calculated from the luteolin peak.
4.2 taking a reference solution (with the concentration of 11.35 mu g/ml) of luteolin, and diluting 10%, 20% and 50% of the luteolin respectively according to 100%. A control solution of luteolin (21.35 μ g/ml) was reconstituted at 200% and the results are shown in Table 2.
TABLE 2 Linear relationship test data sheet
| Ratio of | 10％ | 20％ | 50％ | 100％ | 200％ |
| Concentration of | 1.135μg/ml | 2.27μg/ml | 5.675μg/ml | 11.35μg/ml | 22.7μg/ml |
| Peak area | 45.753 | 86.782 | 221.135 | 445.408 | 872.335 |
4.3 taking the linear solutions with different concentration levels, respectively sucking 10 mu l of the linear solutions, injecting the linear solutions into a liquid chromatograph, recording a chromatogram, carrying out linear regression analysis on the concentration (x) by using a peak area (y), drawing a linear relation graph, reporting a linear equation, and obtaining a result shown in an attached figure 5 in detail.
4.4 conclusion: under the condition of the sample amount of 10 mu l, the luteolin has good linear relation with the peak area within the concentration range of 1.135 mu g/ml to 22.7 mu g/ml.
5. Precision degree
5.1 repeatability test: octadecylsilane chemically bonded silica is used as a filler in chromatographic conditions and system applicability tests; in acetonitrile-1.0% (v/v) formic acid solution (30: 70); the detection wavelength was 348 nm. The number of theoretical plates should be not less than 4000, calculated from the luteolin peak.
5.1.1 taking 30 granules of the product, taking about 1.2g of the product per part, precisely weighing, processing 6 parts of samples in parallel under 1.2.3, calculating an average result, and requiring 6 parts of results to be less than 4% by RSD according to the guidelines of the ChP' method for analyzing drug quality Standard (Tong rule 9101) of 2015 edition, wherein the results meet the regulations. (results and map viewing intermediate precision under item 5.1.2)
5.1.2 conclusion demonstrates that the method is well reproducible.
5.2 intermediate precision
5.2.1 chromatographic conditions and system applicability test with octadecylsilane chemically bonded silica as filler; in acetonitrile-1.0% (v/v) formic acid solution (30: 70); the detection wavelength was 348 nm. The number of theoretical plates should be not less than 4000, calculated from the luteolin peak.
5.2.2 in the same laboratory, different experimenters A/B perform repeated experiments on different dates by using different chromatographs, record chromatograms and calculate the content by an external standard method.
TABLE 3 intermediate precision test data sheet
5.2.3 results show that the RSD of the luteolin content measured by 6 parts of test solution is 1.4 percent, and the results of 12 parts of solution with repeatability and intermediate precision are compared statistically
The result of 5.2.4 shows that the RSD value of the detection result of 12 test sample solutions is 1.1 percent and meets the specification.
5.2.5 conclusion: the method has good precision.
6. Accuracy (recovery) test
6.1 chromatographic conditions and system applicability test with octadecylsilane chemically bonded silica as filler; with a solution of formic acid in acetonitrile-1.0% (v/v) (30: 70); the detection wavelength was 348 nm. The number of theoretical plates should be not less than 4000, calculated from the luteolin peak.
6.2 preparation of control solutions: the concentration of the preparation is 1.3521 mg/ml.
Preparation of a test solution, precisely weighing prostate capsule particles, precisely weighing about 1.2g and 9 parts respectively, placing into a 50ml conical flask with a plug, precisely adding luteolin reference solution in different amounts and three parts respectively, and treating according to a method of 1.2.3 to obtain the product;
percent recovery is (C-A)/BX 100%
Wherein A is the measured component content of the test sample; b is the amount of the added reference substance; c is an observed value. The standard requires that: the recovery rate is required to be 90-108%. The recovery rate RSD is less than 3.0 percent
TABLE 4 recovery test data sheet
7. Durability
7.1 chromatographic conditions and system applicability test with octadecylsilane chemically bonded silica as filler; in acetonitrile-1.0% (v/v) formic acid solution (30: 70); the detection wavelength was 348 nm. The number of theoretical plates should be not less than 4000, calculated from the luteolin peak.
7.2 the test solution under the repeatability item is placed at room temperature for 0, 4, 8, 12, 18 and 24 hours respectively, and 10 mul of the test solution is precisely absorbed and injected into a high performance liquid chromatograph.
TABLE 5 repeatability test data sheet
| Sample introduction time point | Luteolin retention time | Luteolin Peak area |
| 0h | 28.28 | 457.553 |
| 4h | 28.327 | 443.304 |
| 8h | 28.2 | 437.699 |
| 12h | 28.06 | 445.108 |
| 18h | 27.873 | 445.118 |
| 24h | 27.927 | 447.149 |
| Mean value of | 28.111 | 445.989 |
| RSD | 0.67％ | 1.46％ |
The results of 7.3 showed that the retention time RSD of the test solution was 0.67% within 24 hours, and the RSD of the peak area was 1.46%, which were less than 2.0%.
7.4 conclusion: the test solution is stable within 24 hours at room temperature.
Example three: content determination and stability test of luteolin in patrinia scabiosaefolia link capsule
Samples from the large-scale production of Qianlieping (lot numbers: 20180101, 20180102, 20180103, manufactured by Seikaga Povida GmbH) were subjected to an accelerated test and a room-temperature standing test. Accelerated experiment, placing the sample in a closed environment with relative humidity of 75% +/-5% and temperature of 40 +/-2 ℃ for 6 months, sampling in 0, 1, 2, 3 and 6 months for character observation and microorganism limit detection; and (3) placing at room temperature for experiment, namely placing in a closed environment with the relative humidity of 60 +/-5% and the temperature of 25 +/-2 ℃ for 3 months, sampling and checking at 0, 3, 6, 12 and 18 months, wherein the experiment result is shown in a table 6, and only the content data of luteolin is reserved in the experiment.
TABLE 6 stability test data sheet for luteolin content in Qianliping Capsule
The experimental results show that the content of the luteolin is not significantly changed after the accelerated test is carried out for 6 months and the luteolin is placed at room temperature for 18 months, the requirements are met, the method for detecting the content of the luteolin is applied, the quality of the medicine is more effectively controlled, and the method is considered to be included in the quality standard in the next step.
The embodiments given above are preferable examples for implementing the present invention, and the present invention is not limited to the above-described embodiments. Any non-essential addition and replacement made by the technical characteristics of the technical scheme of the invention by a person skilled in the art belong to the protection scope of the invention.
Claims (2)
1. A method for measuring the content of a Qianlieping capsule is characterized in that the content of luteolin in the North Patrinia is measured by adopting a liquid chromatography, and the method specifically comprises the following steps:
(1) conditions of liquid chromatography
Octadecylsilane chemically bonded silica gel column is used as a filling agent; the volume ratio is 30:70 acetonitrile-1.0% formic acid solution as mobile phase; an ultraviolet detector with the detection wavelength of 350 +/-2 nm; the flow rate is 1.0 ml/min; the column temperature is 35 ℃; the number of theoretical plates is not less than 4000 calculated according to luteolin peak;
(2) preparation of control solutions
Accurately weighing appropriate amount of luteolin control, drying in phosphorus pentoxide desiccator for 24 hr, adding methanol to obtain solution containing 10 μ g per lml;
(3) preparation of test solution
Precisely weighing 0.8-1.5g of sample under the prostate capsule loading term, Soxhlet extracting with 50-300ml of methanol for 1-3h, filtering, washing residue, mixing filtrates, evaporating to dryness, precisely adding 25ml of methanol, shaking up, taking 10ml of subsequent filtrate, adding 10ml of acetone, standing to obtain supernatant, and filtering with 0.45 μm microporous membrane to obtain sample solution;
(4) measurement of
Precisely sucking 10-20 μ l of each of the reference solution and the sample solution, respectively, injecting into high performance liquid chromatograph, and measuring to obtain measurement result.
2. The method for measuring the content of the prostate capsule according to claim 1, wherein: the preparation method of the test solution comprises the following steps: precisely weighing 1.2g of a sample under the prostate capsule loading term, performing Soxhlet extraction with 200ml of methanol for 2h, filtering, washing residues, combining filtrates, evaporating to dryness, precisely adding 25ml of methanol, shaking uniformly, taking 10ml of subsequent filtrate, adding 10ml of acetone, standing to obtain a supernatant, and filtering with a 0.45 mu m microporous filter membrane to obtain a sample solution.
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| CN201911086535.4A CN110763782B (en) | 2019-11-08 | 2019-11-08 | Content determination method of Qianlieping capsules |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20180001747A (en) * | 2016-06-28 | 2018-01-05 | 건국대학교 산학협력단 | Metabolite marker for identifying raw material of fermented red pepper paste and use thereof |
| CN107998166A (en) * | 2017-12-26 | 2018-05-08 | 湖南知达医药科技有限公司 | The preparation method of dandelion extract |
| CN108132317A (en) * | 2018-02-27 | 2018-06-08 | 吉林农业大学 | A kind of method of flavones ingredient content in measure corn stigma |
| CN109030678A (en) * | 2018-07-18 | 2018-12-18 | 天津中医药大学 | The content assaying method of 5 kinds of chemical components in a kind of northeast Chinese Ixeris |
| CN110075179A (en) * | 2019-05-15 | 2019-08-02 | 青海普兰特药业有限公司 | A kind of spot lip resupinate woodbetony leaf or root granule, preparation method and its detection method |
-
2019
- 2019-11-08 CN CN201911086535.4A patent/CN110763782B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20180001747A (en) * | 2016-06-28 | 2018-01-05 | 건국대학교 산학협력단 | Metabolite marker for identifying raw material of fermented red pepper paste and use thereof |
| CN107998166A (en) * | 2017-12-26 | 2018-05-08 | 湖南知达医药科技有限公司 | The preparation method of dandelion extract |
| CN108132317A (en) * | 2018-02-27 | 2018-06-08 | 吉林农业大学 | A kind of method of flavones ingredient content in measure corn stigma |
| CN109030678A (en) * | 2018-07-18 | 2018-12-18 | 天津中医药大学 | The content assaying method of 5 kinds of chemical components in a kind of northeast Chinese Ixeris |
| CN110075179A (en) * | 2019-05-15 | 2019-08-02 | 青海普兰特药业有限公司 | A kind of spot lip resupinate woodbetony leaf or root granule, preparation method and its detection method |
Non-Patent Citations (2)
| Title |
|---|
| HPLC 同时测定白毛夏枯草中木犀草素与芹菜素的含量;李卫文等;《现代中药研究与实践》;20161231;第30卷(第5期);第56页第2节 * |
| 花生壳提取物中木犀草素及总黄酮含量的测定;李明静等;《化学研究》;20050630;第16卷(第2期);第76,92页第1-2节 * |
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