CN110749593A - Method for rapidly detecting residual quantity of sulfur dioxide in food - Google Patents
Method for rapidly detecting residual quantity of sulfur dioxide in food Download PDFInfo
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- CN110749593A CN110749593A CN201911078739.3A CN201911078739A CN110749593A CN 110749593 A CN110749593 A CN 110749593A CN 201911078739 A CN201911078739 A CN 201911078739A CN 110749593 A CN110749593 A CN 110749593A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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Abstract
The invention discloses a method for rapidly detecting the residual quantity of sulfur dioxide in food, and belongs to the technical field of rapid detection of food safety. And extracting the sample by using an extracting solution, and performing color development treatment, and then comparing the semi-quantitative sulfur dioxide residue with that of a standard colorimetric card. The detection method is rapid, convenient, simple to operate and low in cost, and can meet the requirements of on-site rapid detection and laboratory detection of individuals, enterprises, quality supervision, industrial and commercial departments and non-professionals.
Description
Technical Field
The invention belongs to the technical field of rapid detection of food safety, relates to a rapid detection method of sulfur dioxide residue in food, and particularly relates to a rapid detection method of sulfur dioxide residue in semi-quantitative food by using a standard colorimetric card.
Background
Sulfur dioxide in food is commonly referred to as sulfur dioxide and inorganic sulfites capable of generating sulfur dioxide, and includes sulfur dioxide, potassium metabisulfite, sodium sulfite, sodium bisulfite, sodium hyposulfite, etc., and is a bleaching agent, preservative and antioxidant widely used in the food industry.
However, since the 80's of the 20 th century, the safety of sulfur dioxide in food products has attracted increasing attention. Sulfur dioxide and its derivatives are harmful to the respiratory system of humans, and also cause pathological changes in the brain, liver, spleen and kidney, and even to the reproductive system. Therefore, with the enhancement of food safety awareness of people and the increase of food safety monitoring strength in China, sulfur dioxide becomes one of important items for food safety detection.
The acid dichromate method has been used to determine the sulfur dioxide content of air, but is clearly not suitable for the detection of sulfur dioxide in food products based on this method. Therefore, the invention aims to establish a method for rapidly detecting the residual quantity of sulfur dioxide in semi-quantitative food by using a standard colorimetric card through innovative optimization by using the basic principle of determining sulfur dioxide by using an acid dichromate method.
Disclosure of Invention
The invention provides a method for rapidly detecting the residual quantity of sulfur dioxide in food, which utilizes a standard colorimetric card to rapidly and semi-quantitatively detect the sulfur dioxide in the food, and has the advantages of simple operation and low cost.
In order to achieve the purpose, the technical scheme adopted by the invention comprises the following steps: weighing 1g of a sample to be detected, adding 10mL of the extracting solution, uniformly mixing and standing for 5 min; 1mL of supernatant is taken, and a dichromate solution and a sulfuric acid solution are sequentially added and uniformly mixed; adding a diphenylcarbazide acetone solution, uniformly mixing and standing for 5-10 min; and semi-quantifying the residual quantity of the sulfur dioxide in the sample by comparing with a standard colorimetric card.
Preferably, the extract is a phosphate buffer at pH 1.
Preferably, the concentration of the dichromate solution is 0.25mmol/L, and the addition amount is 1 drop; the concentration of the sulfuric acid solution is 4.5mol/L, and the addition amount is 1 drop; the concentration of the dibenzoyl dihydrazido acetone solution is 5.16mmol/L, and the addition amount is 1 drop.
Preferably, the standard colorimetric card is prepared by taking 1mL of sulfite standard solution diluted by the extracting solution to be 0, 2, 5, 10 and 20mg/L, and treating the solution in the same pretreatment mode as the sample to change the purple color into colorless; wherein the concentration is measured as sulfur dioxide.
Has the advantages that: the invention relates to a method for rapidly detecting the residual quantity of sulfur dioxide in food, which comprises the steps of extracting a sample through an extracting solution, uniformly mixing and standing, adding a color developing agent into supernate for color development, and semiquantifying the sulfur dioxide content in the sample by using a standard colorimetric card. The detection method is rapid, convenient, simple to operate and low in cost, and can meet the requirements of on-site rapid detection and laboratory detection of individuals, enterprises, quality supervision, industrial and commercial departments and non-professionals.
Drawings
FIG. 1 is a standard colorimetric card
Wherein: 1. a colorimetric map; 2. the concentration of sulfur dioxide.
Detailed Description
The technical solutions of the present invention are described in detail below with reference to specific examples, but the following examples are only intended to illustrate the present invention in detail and do not limit the scope of the present invention in any way.
The reagents referred to in the following examples are all commercially available conventional reagents unless otherwise specified.
Example one
a. Selecting soaked ginger, dried black fungus, dried bean curd stick and pickled vegetable as samples to be detected, weighing 1g of the samples, adding 10mL of phosphate buffer solution with the pH value of 1, uniformly mixing and standing for 5 min.
b. Taking 1mL of supernatant, sequentially adding 1 drop of 0.25mmol/L dichromate solution and 1 drop of 4.5mol/L sulfuric acid solution, and uniformly mixing; then adding 1 drop of 5.16mmol/L diphenylcarbodihydrazide acetone solution, uniformly mixing and standing for 5-10 min.
c. And semi-quantifying the residual quantity of the sulfur dioxide in the sample by comparing with a standard colorimetric card.
The samples to be detected are respectively detected according to the method of GB 5009.34-2016 (determination of sulfur dioxide in national food safety standards), the detection results are compared with the detection results of the invention, and the qualification judgment is carried out on the detection results of the samples by combining the limit values of the sulfur dioxide residue of various food matrixes in GB 2760-.
TABLE 1 comparison of the sulfur dioxide residue in the samples of the present invention and the national standard
As can be seen from the above table, the invention can semi-quantitatively detect the sulfur dioxide residue in the food sample to be detected, the detection result is consistent with the detection result of the use national standard method, and the detection range of the invention basically accords with the sulfur dioxide residue limit of various food substrates specified in GB 2760-.
Claims (4)
1. A method for rapidly detecting the residual quantity of sulfur dioxide in food is characterized by comprising the following steps: weighing 1g of a sample to be detected, adding 10mL of the extracting solution, uniformly mixing and standing for 5 min; 1mL of supernatant is taken, and a dichromate solution and a sulfuric acid solution are sequentially added and uniformly mixed; adding a diphenylcarbazide acetone solution, uniformly mixing and standing for 5-10 min; and semi-quantifying the residual quantity of the sulfur dioxide in the sample by comparing with a standard colorimetric card.
2. The method according to claim 1, wherein the extract is phosphate buffer with pH of 1.
3. The method for rapidly detecting the residual amount of sulfur dioxide in food according to claim 1, wherein the concentration of the dichromate solution is 0.25mmol/L, and the addition amount is 1 drop; the concentration of the sulfuric acid solution is 4.5mol/L, and the addition amount is 1 drop; the concentration of the dibenzoyl dihydrazido acetone solution is 5.16mmol/L, and the addition amount is 1 drop.
4. The method according to claim 1, wherein the standard colorimetric card is prepared by diluting 1mL of each of the solutions to a sulfite standard solution with a concentration of 0, 2, 5, 10, or 20mg/L, and treating the solution in the same pretreatment manner as the sample to show a color change from purple to colorless; the concentration is expressed in terms of sulfur dioxide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201911078739.3A CN110749593A (en) | 2019-11-02 | 2019-11-02 | Method for rapidly detecting residual quantity of sulfur dioxide in food |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911078739.3A CN110749593A (en) | 2019-11-02 | 2019-11-02 | Method for rapidly detecting residual quantity of sulfur dioxide in food |
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| CN110749593A true CN110749593A (en) | 2020-02-04 |
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| CN201911078739.3A Pending CN110749593A (en) | 2019-11-02 | 2019-11-02 | Method for rapidly detecting residual quantity of sulfur dioxide in food |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102128862A (en) * | 2010-01-18 | 2011-07-20 | 惠碁生物科技股份有限公司 | Detecting method, detecting test piece and detector for redox materials in food |
| CN107449773A (en) * | 2017-09-18 | 2017-12-08 | 贵州遵义博源环境科技检测服务有限公司 | A kind of sulfur dioxide quick detection suit |
| CN107462575A (en) * | 2017-07-25 | 2017-12-12 | 广东省药品检验所（广东省药品质量研究所、广东省口岸药品检验所） | A kind of sulfur dioxide quick detection kit and detection method |
| CN107764815A (en) * | 2017-09-29 | 2018-03-06 | 郑州欧柯奇仪器制造有限公司 | The method of quick detection Nitrate Content in Food |
-
2019
- 2019-11-02 CN CN201911078739.3A patent/CN110749593A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102128862A (en) * | 2010-01-18 | 2011-07-20 | 惠碁生物科技股份有限公司 | Detecting method, detecting test piece and detector for redox materials in food |
| CN107462575A (en) * | 2017-07-25 | 2017-12-12 | 广东省药品检验所（广东省药品质量研究所、广东省口岸药品检验所） | A kind of sulfur dioxide quick detection kit and detection method |
| CN107449773A (en) * | 2017-09-18 | 2017-12-08 | 贵州遵义博源环境科技检测服务有限公司 | A kind of sulfur dioxide quick detection suit |
| CN107764815A (en) * | 2017-09-29 | 2018-03-06 | 郑州欧柯奇仪器制造有限公司 | The method of quick detection Nitrate Content in Food |
Non-Patent Citations (2)
| Title |
|---|
| 孙成均: ""二氧化硫非汞盐吸收比色分析法研究的进展"", 《预防医学情报杂志》 * |
| 李峰等: ""二苯碳酰二肼分光光度法测定空气中二氧化硫"", 《光谱实验室》 * |
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