CN110746332A - Production method of lutein and nostoc composite powder - Google Patents
Production method of lutein and nostoc composite powder Download PDFInfo
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- CN110746332A CN110746332A CN201911100719.1A CN201911100719A CN110746332A CN 110746332 A CN110746332 A CN 110746332A CN 201911100719 A CN201911100719 A CN 201911100719A CN 110746332 A CN110746332 A CN 110746332A
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- 241000192656 Nostoc Species 0.000 title claims abstract description 57
- 239000000843 powder Substances 0.000 title claims abstract description 47
- KBPHJBAIARWVSC-RGZFRNHPSA-N lutein Chemical compound C([C@H](O)CC=1C)C(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\[C@H]1C(C)=C[C@H](O)CC1(C)C KBPHJBAIARWVSC-RGZFRNHPSA-N 0.000 title claims abstract description 43
- 229960005375 lutein Drugs 0.000 title claims abstract description 43
- KBPHJBAIARWVSC-XQIHNALSSA-N trans-lutein Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2C(=CC(O)CC2(C)C)C KBPHJBAIARWVSC-XQIHNALSSA-N 0.000 title claims abstract description 43
- FJHBOVDFOQMZRV-XQIHNALSSA-N xanthophyll Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2C=C(C)C(O)CC2(C)C FJHBOVDFOQMZRV-XQIHNALSSA-N 0.000 title claims abstract description 43
- 235000012680 lutein Nutrition 0.000 title claims abstract description 40
- ORAKUVXRZWMARG-WZLJTJAWSA-N lutein Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=CC(O)CC2(C)C)C ORAKUVXRZWMARG-WZLJTJAWSA-N 0.000 title claims abstract description 40
- 239000001656 lutein Substances 0.000 title claims abstract description 40
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 24
- 239000002131 composite material Substances 0.000 title claims abstract description 19
- 238000000605 extraction Methods 0.000 claims abstract description 19
- 239000002904 solvent Substances 0.000 claims abstract description 12
- 239000000463 material Substances 0.000 claims description 50
- 238000005406 washing Methods 0.000 claims description 44
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 23
- OSWPMRLSEDHDFF-UHFFFAOYSA-N methyl salicylate Chemical compound COC(=O)C1=CC=CC=C1O OSWPMRLSEDHDFF-UHFFFAOYSA-N 0.000 claims description 20
- 235000005881 Calendula officinalis Nutrition 0.000 claims description 18
- 241000736851 Tagetes Species 0.000 claims description 18
- 239000012153 distilled water Substances 0.000 claims description 17
- 239000007788 liquid Substances 0.000 claims description 15
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 claims description 12
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- 239000009637 wintergreen oil Substances 0.000 claims description 12
- PAYRUJLWNCNPSJ-UHFFFAOYSA-N Aniline Chemical compound NC1=CC=CC=C1 PAYRUJLWNCNPSJ-UHFFFAOYSA-N 0.000 claims description 10
- 108010059892 Cellulase Proteins 0.000 claims description 10
- 229940106157 cellulase Drugs 0.000 claims description 10
- 239000000243 solution Substances 0.000 claims description 10
- 229920000742 Cotton Polymers 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 8
- 238000000926 separation method Methods 0.000 claims description 8
- 238000002791 soaking Methods 0.000 claims description 8
- 238000004043 dyeing Methods 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 7
- 240000001131 Nostoc commune Species 0.000 claims description 6
- 235000013817 Nostoc commune Nutrition 0.000 claims description 6
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- 230000008859 change Effects 0.000 claims description 5
- 239000011259 mixed solution Substances 0.000 claims description 5
- 239000008399 tap water Substances 0.000 claims description 5
- 235000020679 tap water Nutrition 0.000 claims description 5
- LCPUDZUWZDSKMX-UHFFFAOYSA-K azane;hydrogen sulfate;iron(3+);sulfate;dodecahydrate Chemical compound [NH4+].O.O.O.O.O.O.O.O.O.O.O.O.[Fe+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O LCPUDZUWZDSKMX-UHFFFAOYSA-K 0.000 claims description 4
- UXTMROKLAAOEQO-UHFFFAOYSA-N chloroform;ethanol Chemical compound CCO.ClC(Cl)Cl UXTMROKLAAOEQO-UHFFFAOYSA-N 0.000 claims description 4
- 239000000084 colloidal system Substances 0.000 claims description 4
- 238000005520 cutting process Methods 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 229960001047 methyl salicylate Drugs 0.000 claims description 4
- 238000004806 packaging method and process Methods 0.000 claims description 4
- 239000002245 particle Substances 0.000 claims description 4
- 239000004576 sand Substances 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 4
- 238000001291 vacuum drying Methods 0.000 claims description 4
- 230000001680 brushing effect Effects 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- 238000003825 pressing Methods 0.000 claims description 3
- 238000010008 shearing Methods 0.000 claims description 3
- 235000008210 xanthophylls Nutrition 0.000 claims description 3
- 241000628997 Flos Species 0.000 claims description 2
- 230000018044 dehydration Effects 0.000 claims description 2
- 238000006297 dehydration reaction Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 8
- 230000005855 radiation Effects 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 3
- 238000002137 ultrasound extraction Methods 0.000 abstract description 3
- 230000001133 acceleration Effects 0.000 abstract description 2
- 230000000149 penetrating effect Effects 0.000 abstract description 2
- 238000003756 stirring Methods 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 6
- 229940037003 alum Drugs 0.000 description 5
- 241000192700 Cyanobacteria Species 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 230000000007 visual effect Effects 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 241000192543 Nostocaceae Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 208000001491 myopia Diseases 0.000 description 2
- 230000004379 myopia Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000008096 xylene Substances 0.000 description 2
- 244000146462 Centella asiatica Species 0.000 description 1
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- 238000000862 absorption spectrum Methods 0.000 description 1
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- 235000006708 antioxidants Nutrition 0.000 description 1
- 235000021466 carotenoid Nutrition 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
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- 235000013399 edible fruits Nutrition 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 125000003690 ionone group Chemical group 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
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- 239000013589 supplement Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C403/00—Derivatives of cyclohexane or of a cyclohexene or of cyclohexadiene, having a side-chain containing an acyclic unsaturated part of at least four carbon atoms, this part being directly attached to the cyclohexane or cyclohexene or cyclohexadiene rings, e.g. vitamin A, beta-carotene, beta-ionone
- C07C403/24—Derivatives of cyclohexane or of a cyclohexene or of cyclohexadiene, having a side-chain containing an acyclic unsaturated part of at least four carbon atoms, this part being directly attached to the cyclohexane or cyclohexene or cyclohexadiene rings, e.g. vitamin A, beta-carotene, beta-ionone having side-chains substituted by six-membered non-aromatic rings, e.g. beta-carotene
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/06—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/30—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
- A23L5/32—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation using phonon wave energy, e.g. sound or ultrasonic waves
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C2601/00—Systems containing only non-condensed rings
- C07C2601/12—Systems containing only non-condensed rings with a six-membered ring
- C07C2601/16—Systems containing only non-condensed rings with a six-membered ring the ring being unsaturated
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Mycology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention relates to the technical field of lutein and nostoc composite powder, and discloses a production method of the lutein and nostoc composite powder, wherein the formula of the lutein and nostoc composite powder is as follows: the production method of the lutein-nostoc composite powder comprises the steps of adopting ultrasonic-assisted extraction during extraction, utilizing multistage effects such as strong cavitation effect, disturbance effect, high acceleration, crushing, stirring and the like generated by ultrasonic radiation pressure to accelerate the molecular motion frequency and speed of a substance, increasing the penetrating force of a solvent, accelerating the entry of a target component into the solvent, and further improving the extraction rate of lutein, thereby further improving the quality of a produced product.
Description
Technical Field
The invention relates to the technical field of lutein and nostoc composite powder, in particular to a production method of lutein and nostoc composite powder.
Background
Nostoc commune (Nostoc commune) belongs to the phylum Cyanophyta (Cyanophyta), the class Cyanophyceae (Cyanophyceae), the order Nostoccales (Nostoccales), the family Nostocaceae (Nostocaceae), Nostoc commune Vauch in Nostoc, and is the Colony type (Colony) in Nostoc. Commonly named as nostoc commune, and centella asiatica.
Lutein (Lutein), also known as Lutein, is widely present in vegetables, fruits, flowers and other plants, contains two ionone rings in its chemical formula, is a carotenoid with vitamin a activity, is a main pigment present in the macular region of human eyes, and is one of fat-soluble vitamins, and its absorption spectrum contains near-blue purple light, which can help the retina of eyes to resist ultraviolet rays. Lutein is an important antioxidant for the eye to play a role. The lutein supplement is beneficial to maintaining the visual endurance, improving the visual response time and reducing the visual injury. For the myopia, the increase of the myopia degree can be delayed by supplementing lutein, and the damage of computer radiation to the human body can be effectively prevented by frequently using the lutein.
However, most of the production methods or preparation processes of lutein and nostoc composite powder in the current market have low extraction rate of lutein, which causes the phenomenon of raw material waste, so that a production method of lutein and nostoc composite powder with high production quality is urgently needed.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a production method of lutein and nostoc composite powder, which has the advantages of high production or product quality and the like.
(II) technical scheme
In order to achieve the purpose, the invention provides the following technical scheme: a production method of lutein and nostoc composite powder comprises the following steps: dried flower powder of marigold and nostoc commune;
the particle size of the marigold dried flower powder is 60 meshes, and the marigold dried flower powder is treated by cellulase for 16 hours.
Extracting the dry marigold flower powder treated by cellulase for 2 times by using chloroform-ethanol (3: 2) mixed solution as an extraction solvent, wherein the material-liquid ratio is 1: 10, and then saponifying with 5% sodium hydroxide solution for 8 hours.
Sequentially filtering, concentrating under reduced pressure, extracting, crystallizing and separating the saponified flos Tagetis Erectae dried flower powder to obtain xanthophyll for use.
The extraction solvent can also be n-hexane, and the material-liquid ratio is 1: 4, extracting for 2 times at the temperature of 40 ℃, extracting for 3 hours for the first time, and extracting for 2 hours for the second time.
Preferably, ultrasonic-assisted extraction is adopted during extraction, and the ultrasonic power is 300W.
Preferably, the common nostoc is soaked in water, when a colloid sheath of the common nostoc expands, mud, sand and sundries are cleaned by a small brush, the common nostoc is cut into small blocks (or sections) with the length of about 3 mm, the flat nostoc small blocks or sections are selected to be placed in the center of a slide, then the material is thinned by another slide, two slides are tied by a cotton thread, the two slides are tied, the tied two slides are put into FAA fixing solution for 2 hours, then the cotton thread is loosened, the slides are separated, the material is dyed and washed by dehydration, and is primarily dyed, the fixed material is washed by 50% alcohol for 2 to 3 times, each time is 10 minutes, then is washed by 1%, 3%, 20% and 10% alcohol, each stage is 10 minutes, finally is washed by distilled water for 2 times, each time is washed by 2 minutes, each time is washed by 4% iron alum for 1 hour by distilled water, then is dyed by Hai's hematoxylin 1 hour distilled water for 1 hour, then the alum is washed by 1% iron with 1% for color separation, immediately pouring out the distilled water of the color separation liquid until the other parts are light gray or colorless, washing for 2 to 3 times, soaking the material in tap water to change the color into blue, washing the dehydrated and counterdyed material with 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80% and 95% alcohol for 10 minutes each stage, adding a plurality of drops of aniline into pure alcohol for counterdyeing until the cells are blue-green, washing off the floating color with the pure alcohol, placing the material in the mixed liquid of wintergreen oil (methyl salicylate) and the pure alcohol for 15 minutes, placing the material in the pure wintergreen oil for 30 minutes, replacing the wintergreen oil once, sealing and cutting out the material, immersing the material in dimethylbenzene for a moment, and finally vacuum drying to prepare powder for later use.
Preferably, the nostoc powder to be used is mixed with lutein according to a ratio of 1: and 1.14, mixing, packaging, inspecting and warehousing.
(III) advantageous effects
Compared with the prior art, the invention provides the display device for the online teaching based on the intelligent control, which has the following beneficial effects:
1. according to the production method of the lutein and nostoc composite powder, the marigold dried flower powder is treated by the cellulase for 16 hours, the cellulase can destroy plant cell walls, so that plants in cells can be dissolved out better, the lutein is prevented from being damaged in the extraction process of a single organic solvent, and the production quality is improved.
2. According to the production method of the lutein and nostoc composite powder, ultrasonic-assisted extraction is adopted during extraction, and the multi-stage effects such as strong cavitation effect, disturbance effect, high acceleration, crushing and stirring action and the like generated by ultrasonic radiation pressure are utilized to accelerate the molecular motion frequency and speed of a substance and increase the penetrating power of a solvent, so that the target component is accelerated to enter the solvent, the extraction rate of the lutein is improved, and the quality of a produced product is further improved.
3. The production method of the lutein and nostoc composite powder comprises the steps of dyeing nostoc nubs, washing the fixed nostoc nubs with 50% alcohol for 2 to 3 times, washing the nostoc nubs for 10 minutes each time, washing the nostoc nubs with 1%, 3%, 20% and 10% alcohol for 10 minutes each time, finally washing the nostoc nubs with distilled water for 2 times each time, washing the nostoc nubs with 4% iron alum for 2 times each time, then washing the nostoc nubs with distilled water for 1 hour to remove floating color, carrying out color separation by the iron alum 1% while carrying out microscopic examination until the content in cells is colored, immediately pouring the distilled water of a color separation solution until the other parts are light gray or colorless, soaking the materials in tap water after washing for 2 to 3 times to change the materials into blue, and washing the dehydrated and redyed materials with 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80% and 95% alcohol for 10 minutes each time, and then adding a plurality of drops of aniline into the pure alcohol for counterstaining until the cells are blue-green, and then washing away the floating color by using the pure alcohol, so that VC contained in the nostoc and a small amount of VB1, VB2, VA and VE can be effectively ensured not to be damaged and lost, and the nutritional value of the nostoc is maximally preserved.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example one
A production method of lutein and nostoc composite powder comprises the following steps: the method comprises the following steps of (1) extracting marigold dried flower powder and common nostoc, wherein the particle size of the marigold dried flower powder is 60 meshes, the marigold dried flower powder is firstly treated by cellulase for 16 hours, the marigold dried flower powder treated by the cellulase is extracted by using chloroform-ethanol (3: 2) mixed solution as an extraction solvent for 2 times, each time is 2 hours, and the material-liquid ratio is 1: and 10, saponifying with 5% sodium hydroxide solution for 8 hours, sequentially filtering, concentrating under reduced pressure, extracting, crystallizing and separating the saponified marigold dried flower powder to obtain lutein for later use, wherein the extraction solvent can also be n-hexane, and the ratio of material to liquid is 1: and 4, extracting for 2 times at the temperature of 40 ℃, extracting for 3 hours for the first time, extracting for 2 hours for the second time, and extracting by adopting ultrasonic wave for assistance, wherein the power of the ultrasonic wave is 300W.
Soaking common nostoc in water, brushing mud and sand impurities with a small pen when a colloid sheath of the common nostoc expands, shearing the common nostoc into small blocks (or sections) with the length of about 3 mm, selecting flat nostoc small blocks or sections to be placed in the center of a slide, then pressing the material with another slide, binding two slides with a cotton thread, binding the two slides, putting the bound two slides into FAA fixing solution for 2 hours, loosening the cotton thread, separating the slides, dyeing, washing the material with dehydrated water and primary dyeing, washing the fixed material with 50% alcohol for 2 to 3 times, 10 minutes each time, washing with 1%, 3%, 20% and 10% alcohol, 10 minutes each stage, washing with distilled water for 2 times, 2 minutes each time, washing with 4% ferric alum for 1 hour, washing with hematoxylin distilled water for 2 times, washing with hematoxylin essence for 1 hour to remove floating color, separating colors, performing microscopic examination while performing microscopic examination on the 1% ferric alum to obtain intracellular content, immediately pouring out the distilled water of the color separation liquid until the other parts are light gray or colorless, washing for 2 to 3 times, soaking the material in tap water to change the color of the material into blue, washing the dehydrated and counterdyed material by 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80% and 95% alcohol for 10 minutes each stage, then adding a plurality of drops of aniline into pure alcohol for counterdyeing until the cells are blue-green, washing off the floating color by using the pure alcohol, placing the material in a mixed solution of wintergreen oil (methyl salicylate) and the pure alcohol for 15 minutes, placing the material in the pure wintergreen oil for 30 minutes, replacing the wintergreen oil once, sealing and cutting the material out, immersing the material in xylene for a moment, finally performing vacuum drying to prepare powder for standby, and mixing the spare nostoc powder and lutein according to the ratio of 1: and 1.14, mixing, packaging, inspecting and warehousing.
Example two
A production method of lutein and nostoc composite powder comprises the following steps: the method comprises the following steps of (1) extracting marigold dried flower powder and common nostoc, wherein the particle size of the marigold dried flower powder is 40 meshes, the marigold dried flower powder is firstly treated by cellulase for 8 hours, the marigold dried flower powder treated by the cellulase is extracted by using chloroform-ethanol (3: 2) mixed solution as an extraction solvent for 2 times, each time is 2 hours, and the material-liquid ratio is 1: 8, saponifying with 5% sodium hydroxide solution for 4 hr, sequentially filtering, concentrating under reduced pressure, extracting, crystallizing and separating to obtain xanthophyll, wherein the extraction solvent may be n-hexane, and the ratio of material to liquid is 1: and 3, extracting for 2 times at the temperature of 50 ℃, extracting for 3 hours for the first time and extracting for 2 hours for the second time, wherein ultrasonic wave is adopted for auxiliary extraction during extraction, and the power of the ultrasonic wave is 300W.
Soaking common nostoc in water, brushing mud and sand impurities with a small pen when a colloid sheath of the common nostoc expands, shearing the common nostoc into small blocks (or sections) with the length of about 4 mm, selecting flat nostoc small blocks or sections to be placed in the center of a slide, then pressing the material with another slide, binding two slides with a cotton thread, binding the two slides, putting the bound two slides into FAA fixing solution for 1 hour, loosening the cotton thread, separating the slides, dyeing, washing the material with dehydrated water and primary dyeing, washing the fixed material with 50% alcohol for 2 to 3 times, 10 minutes each time, washing with 1%, 3%, 20% and 10% alcohol, 10 minutes each stage, washing with distilled water for 2 times, 2 minutes each time, washing with 4% ferric alum for 1 hour, washing with hematoxylin distilled water for 2 times, washing with hematoxylin essence for 1 hour to remove floating color, separating colors, performing microscopic examination while performing microscopic examination on the 1% ferric alum to obtain intracellular content, immediately pouring out the distilled water of the color separation liquid until the other parts are light gray or colorless, washing for 2 to 3 times, soaking the material in tap water to change the color of the material into blue, washing the dehydrated and counterdyed material by 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80% and 95% alcohol for 10 minutes each stage, then adding a plurality of drops of aniline into pure alcohol for counterdyeing until the cells are blue-green, washing off the floating color by using the pure alcohol, placing the material in the mixed liquid of wintergreen oil (methyl salicylate) and the pure alcohol for 15 minutes, placing the material in the pure wintergreen oil for 45 minutes, replacing the wintergreen oil once, sealing and cutting the material out, immersing the material in xylene for a moment, finally performing vacuum drying to prepare powder for standby, and mixing the spare nostoc powder and lutein according to the ratio of 1: 1.26 proportion mixing packaging, inspection and warehousing
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. A production method of lutein and nostoc composite powder is characterized by comprising the following steps: the formula of the lutein and nostoc composite powder is as follows: dried flower powder of marigold and nostoc commune;
the particle size of the marigold dried flower powder is 60 meshes, and the marigold dried flower powder is treated by cellulase for 16 hours.
2. The method for producing lutein and nostoc according to claim 1, wherein the method comprises the following steps: extracting the dry marigold flower powder treated by cellulase for 2 times by using chloroform-ethanol (3: 2) mixed solution as an extraction solvent, wherein the material-liquid ratio is 1: 10, and then saponifying with 5% sodium hydroxide solution for 8 hours.
3. The method for producing lutein and nostoc according to claim 2, wherein the method comprises the following steps: sequentially filtering, concentrating under reduced pressure, extracting, crystallizing and separating the saponified flos Tagetis Erectae dried flower powder to obtain xanthophyll for use.
4. The method for producing lutein and nostoc according to claim 2, wherein the method comprises the following steps: the extraction solvent can also be n-hexane, and the material-liquid ratio is 1: 4, extracting for 2 times at the temperature of 40 ℃, extracting for 3 hours for the first time, and extracting for 2 hours for the second time.
5. The method for producing lutein and nostoc according to claim 3, wherein the method comprises the following steps: and ultrasonic wave is adopted for auxiliary extraction during extraction, and the power of the ultrasonic wave is 300W.
6. The method for producing lutein and nostoc according to claim 1, wherein the method comprises the following steps: soaking common nostoc in water, brushing mud and sand impurities with a small pen when a colloid sheath of the common nostoc expands, shearing the common nostoc into small blocks (or sections) with the length of about 3 millimeters, selecting flat nostoc small blocks or sections to be placed in the center of a slide, then pressing the material with another slide, binding two slides with a cotton thread, binding the two slides, putting the bound two slides into FAA fixing solution for 2 hours, loosening the cotton thread, separating the slides, dyeing, washing the material with dehydration, washing the material with primary dyeing, washing the fixed material with 50% alcohol for 2 to 3 times, washing the material with 10% alcohol for 10 minutes each time, washing the material with 1%, 3%, 20% and 10% alcohol for 10 minutes each time, washing the material with distilled water for 2 times, washing the material with 2 minutes each time, washing the material with 4% hematoxylin distilled water for 1 hour, washing the material with Hai's fine distilled water for 1 hour for color separation, performing microscopic examination on the cell contents while performing microscopic examination on the 1% iron alum, immediately pouring out the distilled water of the color separation liquid until the other parts are light gray or colorless, washing for 2 to 3 times, soaking the material in tap water to change the color into blue, washing the dehydrated and counterdyed material with 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80% and 95% alcohol for 10 minutes each stage, adding a plurality of drops of aniline into pure alcohol for counterdyeing until the cells are blue-green, washing off the floating color with the pure alcohol, placing the material in the mixed liquid of wintergreen oil (methyl salicylate) and the pure alcohol for 15 minutes, placing the material in the pure wintergreen oil for 30 minutes, replacing the wintergreen oil once, sealing and cutting out the material, immersing the material in dimethylbenzene for a moment, and finally vacuum drying to prepare powder for later use.
7. The method for producing lutein and nostoc according to claim 6, wherein the method comprises the following steps: mixing the nostoc powder for standby with lutein according to the ratio of 1: and 1.14, mixing, packaging, inspecting and warehousing.
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| WO2022157805A1 (en) * | 2021-01-20 | 2022-07-28 | Council Of Scientific And Industrial Research | A process for extraction of biomolecules from biomass |
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