CN110734900B - Cytosine base editing tool and application thereof - Google Patents

Abstract

The invention relates to the technical field of biology, in particular to a cytosine base editing tool and application thereof. The invention provides a fusion protein which comprises an APOBEC3G fragment and an SpCas9-D10A nickase fragment. The APOBEC3G series cytosine base editing tool provided by the invention has R24A, W94L, Y124A, W127L and P200K amino acid mutations in an APOBEC3G fragment relative to a wild type, wherein four amino acid mutations of R24A, W94L, Y124A and W127L can limit the combination of APOBEC3G and RNA, four amino acid mutations of D128K, P199A, P200K and Q322K can improve the combination of APOBEC3G and DNA, and in the base editing of mutating C at 4-7 position of sgRNA 5' end into T, the editing efficiency can be improved, and the RNA off-target effect of the cytosine base editing tool can be greatly reduced or even eliminated.

Description

Cytosine base editing tool and application thereof

Technical Field

The invention relates to the technical field of biology, in particular to a cytosine base editing tool and application thereof.

Background

CRISPR/Cas9 is currently the most efficient and convenient genome editing technology. Cas9 nuclease, guided by guide RNA (sgRNA), can reach a specific target of the genome, cleave it, thereby generating DNA Double Strand Breaks (DSB), and then achieve editing through endogenous DNA repair mechanisms. DNA Repair mechanisms include Non-Homologous End joining (NHEJ) and Homologous recombination Repair (HDR). Among them, NHEJ repair results in random insertions, deletions, leading to inactivation of genes, which dominates in genome repair. And the HDR can be accurately repaired by using the template, so that the gene mutation is corrected.

However, the odds of HDR-mediated accurate repair are low, typically less than 5%, thus greatly limiting the application of CRISPR/Cas9 in the conversion from scientific research to application. Especially in the aspect of gene precise treatment, the method is always a great problem in the field of gene editing.

Recently, a newly developed Base Editor (BE) has successfully solved the above problems, and the efficiency of correcting gene mutation has been greatly improved. The existing Base editors include a Cytosine Base Editor (CBE) and an Adenine Base Editor (ABE).

CBE and ABE are formed by integrating RuvC domain inactivated Cas9D10Anickase (nCas9) and cytosine deaminase/adenine deaminase, reaching a target site under the guidance of sgRNA and combining with a DNA chain which is complementary to the sgRNA, wherein the cytosine deaminase deaminates a certain range of peripheral cytosine C into uracil U, the U can be complementarily paired with cytosine A, and the U is finally replaced by a complementary pairing base T of A through DNA replication; similarly, adenine deaminase deaminates a peripheral range of adenine A into hypoxanthine I, which can be complementarily paired with cytosine C, and through DNA replication I will eventually be replaced by the complementary pairing base G of C. Thereby achieving the purpose of C-to-T or A-to-G. Both BE3 and ABE, the first inventions at present, have very wide applications. Wherein, the editing window corresponding to BE3 is 4-8 bits of the 5 'end of the sgRNA, and the editing window corresponding to ABE is 4-7 bits of the 5' end of the sgRNA.

The deaminase rAPOBEC1 in BE3 is a rat cytosine deaminase, wherein the endogenous state of rAPOBEC1 can edit single-stranded DNA, but also RNA, changing C to U, in addition to the above representation. Recent research shows that the BE3 base editor has serious RNA off-Target effect (off-Target) during action ⁵ The application of the base editor is greatly limited.

Disclosure of Invention

The invention aims to provide a cytosine base editing tool and application thereof.

In order to achieve the above object, the present invention provides a fusion protein, which comprises, in order from N-terminus to C-terminus, an APOBEC3G (A3G) fragment and a SpCas9-D10A nickase fragment, wherein the APOBEC3G fragment has cytosine deaminase activity; the APOBEC3G fragment has at least one amino acid mutation of R24A, W94L, Y124A, W127L, D128K, P199A, P199W, P200A, P200K and Q322K or the APOBEC3G fragment is a truncated APOBEC3G fragment which is deleted from the first start codon of APOBEC3G to 190 th or 197 th position.

Preferably, the APOBEC3G fragment is derived from human (Homo sapiens).

Preferably, the nucleotide sequence of the APOBEC3G fragment comprises:

a) a nucleotide sequence shown as SEQ ID NO. 27-36; or the like, or, alternatively,

b) a nucleotide sequence having more than 80% sequence similarity with SEQ ID NO.27-36 and having the functions of the nucleotide sequence defined in a).

More preferably, the nucleotide sequence in b) may have more than 80%, 85%, 90%, 93%, 95%, 97%, or 99% similarity to SEQ ID No.27-36 in a).

More preferably, the nucleotide sequence in b) specifically includes: the nucleotide sequence shown as SEQ ID NO.27-36 is obtained by substituting, deleting or adding one or more (specifically, 1-50, 1-30, 1-20, 1-10, 1-5, 1-3, 1, 2, or 3) amino acid codons, or adding one or more (specifically, 1-50, 1-30, 1-20, 1-10, 1-5, 1-3, 1, 2, or 3) amino acid codons at the N-terminal and/or C-terminal.

Preferably, the nucleotide sequence of the SpCas9-D10A nickase fragment comprises:

c) a nucleotide sequence shown as SEQ ID NO. 37-38; or the like, or, alternatively,

d) a nucleotide sequence having more than 80% sequence similarity with SEQ ID No.37-38 and having the function of the nucleotide sequence defined in d).

More preferably, the nucleotide sequence in d) may have more than 80%, 85%, 90%, 93%, 95%, 97%, or 99% similarity to SEQ ID NO. 37-38.

More preferably, the nucleotide sequence in d) specifically includes a nucleotide sequence shown in SEQ ID No.37-38 obtained by substituting, deleting or adding one or more (specifically, 1-50, 1-30, 1-20, 1-10, 1-5, 1-3, 1, 2, or 3) amino acid codons, or adding one or more (specifically, 1-50, 1-30, 1-20, 1-10, 1-5, 1-3, 1, 2, or 3) amino acid codons at the N-terminal and/or C-terminal.

Preferably, the fusion protein further comprises a nuclear localization signal fragment located at the N-terminus of the APOBEC3G fragment or the C-terminus of the SpCas9-D10A nickase fragment.

More preferably, the nucleotide sequence of the nuclear localization signal fragment is shown as SEQ ID NO. 39.

Preferably, the fusion protein further comprises a flexible linker peptide fragment located at the N-terminus of APOBEC3G fragment, between APOBEC3G fragment and SpCas9-D10A nicase, or at the C-terminus of SpCas9-D10A nicase.

More preferably, the nucleotide sequence of the flexibly linked peptide fragment is as set forth in SEQ ID No. 40-41.

The present invention also provides an isolated polynucleotide encoding the above fusion protein.

The invention also provides a construct, which is characterized by being obtained by inserting the isolated polynucleotide into an expression vector; the polynucleotide sequence of the construct is shown in SEQ ID NO. 1-14.

Preferably, the expression vector includes, but is not limited to, a pCMV expression vector, a pSV2 expression vector, a pGL3 expression vector, and the like.

The invention also provides an expression system, which is characterized in that the expression system is a host cell, and the host cell contains the construct or the isolated polynucleotide integrated in the genome. The host cell can express the fusion protein, and the fusion protein can be matched with the sgRNA, so that the fusion protein can be positioned in a target region, and the base editing of the target region is realized.

Preferably, the host cell is selected from a eukaryotic cell or a prokaryotic cell.

More preferably, the host cell is selected from a mouse cell or a human cell.

Further, the host cell is selected from a mouse brain neuroma cell, a human embryonic kidney cell, a human cervical cancer cell, a human colon cancer cell and a human osteosarcoma cell.

Still further, the host cell of the expression system is selected from the group consisting of N2a cells, HEK293FT cells, Hela cells, HCT116 cells, or U2OS cells.

A base editing tool comprising the fusion protein and a sgRNA.

The invention also provides the application of the base editing tool in gene editing of eukaryotes.

Preferably, the gene editing is base editing of C-to-T at positions 4-7 of the 5' end of the sgRNA in the target region.

APOBEC3G is a member of the human APOBEC family, and can bind to single-stranded DNA or RNA, generate deamination, mutate C to U, and play an important role in antiviral processes. Deamination of APOBEC3G tends to occur in the CC sequence. There are two functional domains of APOBEC3G, and earlier studies suggest that the primary role of the amino terminus is RNA binding, and the primary role of the carboxy terminus is DNA binding, as well as deamination, which is also a common feature of all two-domain APOBECs. Of these, it is noteworthy that APOBEC3G was not considered to have RNA editing function by earlier studies. Recent studies have indicated that there is competition between the DNA and RNA binding domains of APOBEC3G, and unlike previous findings, APOBEC3G was overexpressed and found to have RNA deaminase activity.

Compared with the prior art, the invention has the following beneficial effects:

(1) the invention provides a new generation cytosine base editing tool, which is characterized in that APOBEC3G is connected with a spCas9-D10Anickase fragment, and then the functional domain responsible for RNA combination in APOBEC3G is mutated, the RNA deamination function is damaged, and the DNA deamination activity is improved. Subsequently, the deamination domains of several APOBEC family deaminases were aligned and mutated towards APOBEC3G deaminase to increase its activity. The new generation of cytosine editing tools has a narrower editing window compared with BE3, similar editing efficiency, and extremely low or even completely eliminates RNA off-target activity.

(2) The base editing system provided by the invention widens the targeted range of a genome, can use an NGG sequence as PAM, realizes the C-to-T base of 4-7 sites at the 5' end in a sgRNA target region, has high mutation precision, and can greatly reduce or even eliminate RNA off-target effect.

(3) Compared with the wild type, the APOBEC3G fragment of the invention has amino acid mutations of R24A, W94L, Y124A, W127L and the like, can limit the combination of APOBEC3G and RNA, and can greatly reduce or even eliminate the RNA off-target effect of a cytosine base editing tool in the base editing of mutating C at 4-7 sites of the 5' end of sgRNA into T; in addition, the cytosine base editing tool provided by the invention has higher editing efficiency on DNA than a classical cytosine base editing tool (BE3), greatly improves the mutation accuracy on the premise of ensuring the editing efficiency, and has good industrialization prospect.

Drawings

FIG. 1 shows a schematic structure diagram of APOBEC3G-BE3, APOBEC3G-BE4 series plasmids used in the examples of the present invention;

FIG. 2 shows a statistical chart of the editing capacity of the invention A3G-BE3,191-BE3,198-BE3, BE3 to endogenous gene sites in HEK293T cells; wherein a is A3G-BE3,191-BE3,198-BE3, and a C-to-T editing efficiency statistical chart of the BE3 at HEK293Site 3; b is a statistical chart of RNA off-target efficiency of A3G-BE3,191-BE3,198-BE3 and BE 3; c4, C5 represents the position of C at the target site, counts from PAM distal base; 191-BE3,198-BE3 are truncated APOBEC3G-BE3 deleted from the first to 190 th bits and 197 bit of APOBEC3G, respectively;

FIG. 3 is a statistical chart showing the editing ability of the 4M-BE3, BE3, to the endogenous gene locus in HEK293T cells according to the present invention; wherein a is a C-to-T editing efficiency statistical chart of three sites of 4M-BE3, A3G-BE3, BE3 in HEK293Site3, HEK293Site2 and EMX 1; b is a statistical chart of RNA off-target efficiency of 4M-BE3, A3G-BE3 and BE 3; c3, C4, C5, C6, C8 represent the position of C at the target site, counted from the PAM distal base;

FIG. 4 is a statistical chart showing the editing capacity of a series of mutants based on 4M-BE3 of the present invention to endogenous gene loci in HEK293T cells; wherein a is a structural schematic diagram of a mutation site performed on the basis of 4M-BE 3; b is a C-to-T editing efficiency statistical chart of 7 mutant plasmids and 4M-BE3, wherein BE3 is at three sites of HEK293Site2, HEK293Site3 and EMX 1; c is a C-to-T editing efficiency statistical chart of two combined mutant plasmids and 4M-BE3 on the basis of b, wherein BE3 is at three sites of HEK293Site2, HEK293Site3 and EMX 1; d is a statistical chart of RNA off-target efficiency of two plasmids 4M-BE3,4M + P199A + P200K-BE3,4M + D128K + P199A + P200K-BE3 and BE3 with the highest editing efficiency in b and c; c3, C4, C5, C6, C8 represent the position of C at the target site, counted from the PAM distal base;

FIG. 5 is a statistical chart showing the editing capacity of the optimized plasmids 4M + P199A + P200K-BE4,4M + D128K + P199A + P200K-BE4 in HEK293T cells for endogenous gene loci in the present invention; wherein the content of the first and second substances,

a is a structural schematic diagram of 4M + P199A + P200K-BE4,4M + D128K + P199A + P200K-BE 4;

b is a C-to-T editing efficiency statistical chart of 4M + P199A + P200K-BE4,4M + D128K + P199A + P200K-BE4 at HEK293Site 3;

c is a RNA off-target efficiency statistical chart of 4M + P199A + P200K-BE4,4M + D128K + P199A + P200K-BE4BE 3; c4, C5 represents the position of C at the target site, counted from the PAM distal base.

Detailed Description

Before the present embodiments are further described, it is to be understood that the scope of the invention is not limited to the particular embodiments described below; it is also to be understood that the terminology used in the examples is for the purpose of describing particular embodiments, and is not intended to limit the scope of the present invention; in the description and claims of the present application, the singular forms "a", "an" and "the" include plural referents unless the context clearly dictates otherwise.

When numerical ranges are given in the examples, it is understood that both endpoints of each of the numerical ranges and any value therebetween can be selected unless the invention otherwise indicated. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In addition to the specific methods, devices, and materials used in the examples, any methods, devices, and materials similar or equivalent to those described in the examples may be used in the practice of the invention in addition to the specific methods, devices, and materials used in the examples, in keeping with the knowledge of one skilled in the art and with the description of the invention.

Unless otherwise indicated, the experimental methods, detection methods, and preparation methods disclosed herein all employ techniques conventional in the art of molecular biology, biochemistry, chromatin structure and analysis, analytical chemistry, cell culture, recombinant DNA technology, and related arts. These techniques are well described in the literature and may be found in particular in Sambrook et al, Molecular CLONINGG: a LABORATORY MANUAL, Second edition, Cold SpringG Harbor LABORATORY Press, 1989and Third edition, 2001; ausubel et al, Current PROTOCOLS IN MOLECULAR BIOLOGY, John Wiley & Sons, New York, 1987and pharmaceutical updates; the series METHODS IN ENZYMOLOGY, Academic Press, San Diego; wolffe, CHROMATIN STRUCTURE AND FUNCTION, Third edition, Academic Press, San Diego, 1998; METHOD IN ENZYMOLOGY, Vol.304, Chromatin (P.M. Wassarman and A.P. Wolffe, eds.), Academic Press, San Diego, 1999; and METHODS IN MOLECULAR BIOLOGY, Vol.119, chromatography Protocols (P.B.Becker, ed.) Humana Press, Totowa, 1999, etc.

Example 1

In this example, the RNA binding site DNA binding site and the like in the APOBEC3G part were subjected to point mutation (including R24A, W94L, Y124A, W127L, D128K, P199A, P199W, P200A, P200K, Q322K), or the APOBEC3G fragment was deleted from the first start codon of APOBEC3G to the 190 th or 197 th position, respectively, to obtain a truncated APOBEC3G fragment; C-APOBEC3G-BE3 is constructed by truncating the amino terminal of APOBEC3G, or the part of D10Anickase Cas9 in BE3 is replaced by D10Anickase Cas9 with higher expression efficiency (BE 4).

The related plasmid is shown in figure 1, wherein 4M is R24A, W94L, Y124A and W127 four-point mutation, and A3G (OP) is A3G after optimizing codon.

The construction method of the mutant plasmid used in the examples is as follows: amino acid mutations were introduced into the APOBEC3G portion of the A3G-BE3 plasmid or the A3G-BE4 plasmid by the Mut Express II Fast Mutagenesis Kit V2(Vazyme, C214-02) (FIG. 1).

The constructed plasmids C-191-BE3, C-198-BE3, 4M-BE3,4M + D128K-BE3, 4M + P199A-BE3, 4M + P199W-BE3, 4M + P200A-BE3, 4M + P200K-BE3,4M + Q322K-BE3, 4M + D128K + P199A + P200A-BE3, A3G-BE4max, 4M + P199A + P200K-BE4max, 4M + D128K + P199A + P200K-BE4max, A3G (OP) + P199A + P200K-BE4max, and the sequence is shown in SEQ ID NO. 1-14.

Example 2

In this example, the APOBEC3G series of tools was used to edit the endogenous gene loci on HEK293T cells, and the editing efficiency and RNA off-target efficiency were examined.

2.1 construction of sgRNA plasmid

Selecting 3 human endogenous gene loci, designing sgRNAs, wherein the positions of 3 sgRNAs in a genome are NC-000015.10: 107422339-107422361; NC _ 000013.11: 87944780-87944802; NC _ 000014.9: 72917055-72917077.

The upstream and downstream sequences of sgRNA were ligated to pGL3-U6-sgRNA (Addgene #51133) vector linearized with BsaI (NEB: R0539L) by programmed (95 ℃,5 min; 95 ℃ -85 ℃ at-2 ℃/s; 85 ℃ -25 ℃ at-0.1 ℃/s; hold at 4 ℃). The polynucleotide sequence used is shown in SEQ ID NO. 15-20. The linearization system is shown below: pGL3-U6-sgRNA 2. mu.g; buffer (NEB: R0539L) 6. mu.L; BsaI 2. mu.L; ddH2O was replenished to 60. mu.L. The cleavage was carried out overnight at 37 ℃. The linking system is as follows: t4 ligation buffer (NEB: M0202L) 1. mu.L, linearized vector 20NGG, annealed oligo fragment (10. mu.M) 5. mu.L, T4 ligase (NEB: M0202L) 0.5. mu.L, ddH ₂ O was replenished to 10. mu.L.16 ℃ and ligated overnight. The connected vector is transformed, selected and identified. The positive clones were shaken to extract the plasmid (Axygene: AP-MN-P-250G) and the concentration was determined.

2.2 culture transfection and recovery of cells

HEK293T cells (purchased from ATCC) were inoculated in DMEM high-sugar medium (HyClone, SH30022.01B) supplemented with 10% FBS, containing 1% Penicillin Streptomycin (v/v) (Gibco). When the cell concentration is 80%, the medium is replaced with 10% serum DMEM, and the cells are cultured for 2 hours to restore the optimal state. The amount of plasmid transfected per well was 4 μ g of the APOBEC3G series editing tool plasmid (see FIG. 1) and 2 μ g of sgRNA plasmid, respectively, prepared in example 1. The plasmids were mixed in 250. mu.l of Opti-MEM (Gibco,11058021) medium, respectively. Mu.l of Lipofectamine 2000 transfection reagent (Thermo,11668019) was mixed into 250. mu.l of Opti-MEM medium and mixed well, and left to stand for 5 minutes. The plasmid-mixed Opti-MEM was added to the plasmid-mixed Opti-MEM mixed with Lipofectamine 2000, gently whipped, mixed well, and allowed to stand for 20 minutes. Opti-MEM mixed with plasmid and Lipofectamine 2000 was added to each 6cm plate (80% concentration of cells in the plate for transfection). 6 hours after transfection, the cells were replaced with 10% FBS in DMEM. After 48 hours of transfection, 5% of the cells with the highest positive rate were sorted out, 5000 cells were used for detecting DNA editing efficiency, and the remaining 50 ten thousand cells were used for extracting RNA for detecting RNA off-target efficiency.

2.3DNA editing efficiency detection

The DNA was first lysed to obtain the genome, the lysate consisted of 50mM KCl, 1.5mM MgCl2, 10mM Tris pH 8.0, 0.5% Nonidet P-40, 0.5% Tween 20, 100g/ml protease K. And carrying out PCR amplification on a sequence near the target, purifying an amplification product, and identifying by using a SaNGGer sequencing method. The amplification system was as follows: 2Xbuffer (Vazyme, P505) 25. mu.L; dNTP 1 u L; f (10 pmol/. mu.L) 1. mu.L; r (10 pmol/. mu.L) 1. mu.L; 1 mu L of template; 0.5. mu.L of DNA polymerase (Vazyme, P505); ddH2O was made up to 50. mu.L. The amplified PCR product was purified by the following steps: adding PCR-A (Axygen: AP-PCR-250G) with three times of volume to pass through the column, centrifuging, and centrifuging at 12000 r/min for 1 min; 700 μ LW2 was added and centrifuged for 1 min; discarding the waste liquid, adding 700 mu LW2, and centrifuging for 1 minute; discarding the waste liquid, idling for 1 minute; adding 20 μ L water for elution. The PCR amplification primers used are shown in SEQ ID NO. 21-26. And performing Sanger sequencing on the obtained PCR product by using a PCR amplification one-way primer, and then comparing sequencing results and editing efficiency. The results are shown in FIGS. 2A,3A, and 4.

2.4 detection of RNA off-target Effect efficiency

In RNA detection, Trizol (Vazyme, R401-01) is used for extracting total RNA, and the extraction steps are as follows: adding 1ml of Trizol into each hole, uniformly mixing, collecting, adding 200ul of trichloromethane, fully and uniformly mixing by reversing up and down, centrifuging at the temperature of 4 ℃, and centrifuging at 12000 r/min for 15 minutes; sucking 400ul of supernatant, adding isopropanol with the same volume, reversing the supernatant and mixing uniformly, centrifuging at the temperature of 4 ℃, and centrifuging at 12000 r/min for 10 minutes; discarding the supernatant, adding 1mL of 75% ethanol, reversing the upside down, mixing uniformly, centrifuging at the temperature of 4 ℃, and centrifuging at 12000 r/min for 10 minutes; the supernatant was discarded, air-dried and dissolved in water. 2ug was taken for RNA-seq and the off-target effect was analyzed to obtain the specific off-target (i.e., the number of mutations) and the results are shown in FIGS. 2B,3B, and 5.

From the viewpoint of DNA editing efficiency and RNA off-target efficiency, the APOBEC3G series editing tool has the advantages of high editing efficiency, extremely low off-target efficiency, and even elimination.

In conclusion, the present invention effectively overcomes various disadvantages of the prior art and has high industrial utilization value.

The foregoing embodiments are merely illustrative of the principles and utilities of the present invention and are not intended to limit the invention. Those skilled in the art can modify or change the above-described embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which can be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.

SEQUENCE LISTING

<110> Shanghai science and technology university

<120> cytosine base editing tool and application thereof

<160> 41

<170> PatentIn version 3.5

<210> 1

<211> 8430

<212> DNA

<213> Artificial Sequence

<220>

<223> C-191-BE3

<400> 1

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat ggagattctc 420

agacactcga tggatccaaa gacattcact ttcaacttta acaatgaacc ttgggtcaga 480

ggacggcatg agacttacct gtgttatgag gtggagcgca tgcacaatga cacctgggtc 540

ctgctgaacc agcgcagggg ctttctatgc aaccaggctc cacataaaca cggtttcctt 600

gaaggccgcc atgcagagct gtgcttcctg gacgtgattc ccttttggaa gctggacctg 660

gaccaggact acagggttac ctgcttcacc tcctggagcc cctgcttcag ctgtgcccag 720

gaaatggcta aattcatttc aaaaaacaaa cacgtgagcc tgtgcatctt cactgcccgc 780

atctatgatg atcaaggaag atgtcaggag gggctgcgca ccctggccga ggctggggcc 840

aaaatttcaa taatgacata cagtgaattt aagcactgct gggacacctt tgtggaccac 900

cagggatgtc ccttccagcc ctgggatgga ctagatgagc acagccaaga cctgagtggg 960

aggctgcggg ccattctcca gaatcaggaa aacagcggca gcgagactcc cgggacctca 1020

gagtccgcca cacccgaaag tgataaaaag tattctattg gtttagccat cggcactaat 1080

tccgttggat gggctgtcat aaccgatgaa tacaaagtac cttcaaagaa atttaaggtg 1140

ttggggaaca cagaccgtca ttcgattaaa aagaatctta tcggtgccct cctattcgat 1200

agtggcgaaa cggcagaggc gactcgcctg aaacgaaccg ctcggagaag gtatacacgt 1260

cgcaagaacc gaatatgtta cttacaagaa atttttagca atgagatggc caaagttgac 1320

gattctttct ttcaccgttt ggaagagtcc ttccttgtcg aagaggacaa gaaacatgaa 1380

cggcacccca tctttggaaa catagtagat gaggtggcat atcatgaaaa gtacccaacg 1440

atttatcacc tcagaaaaaa gctagttgac tcaactgata aagcggacct gaggttaatc 1500

tacttggctc ttgcccatat gataaagttc cgtgggcact ttctcattga gggtgatcta 1560

aatccggaca actcggatgt cgacaaactg ttcatccagt tagtacaaac ctataatcag 1620

ttgtttgaag agaaccctat aaatgcaagt ggcgtggatg cgaaggctat tcttagcgcc 1680

cgcctctcta aatcccgacg gctagaaaac ctgatcgcac aattacccgg agagaagaaa 1740

aatgggttgt tcggtaacct tatagcgctc tcactaggcc tgacaccaaa ttttaagtcg 1800

aacttcgact tagctgaaga tgccaaattg cagcttagta aggacacgta cgatgacgat 1860

ctcgacaatc tactggcaca aattggagat cagtatgcgg acttattttt ggctgccaaa 1920

aaccttagcg atgcaatcct cctatctgac atactgagag ttaatactga gattaccaag 1980

gcgccgttat ccgcttcaat gatcaaaagg tacgatgaac atcaccaaga cttgacactt 2040

ctcaaggccc tagtccgtca gcaactgcct gagaaatata aggaaatatt ctttgatcag 2100

tcgaaaaacg ggtacgcagg ttatattgac ggcggagcga gtcaagagga attctacaag 2160

tttatcaaac ccatattaga gaagatggat gggacggaag agttgcttgt aaaactcaat 2220

cgcgaagatc tactgcgaaa gcagcggact ttcgacaacg gtagcattcc acatcaaatc 2280

cacttaggcg aattgcatgc tatacttaga aggcaggagg atttttatcc gttcctcaaa 2340

gacaatcgtg aaaagattga gaaaatccta acctttcgca taccttacta tgtgggaccc 2400

ctggcccgag ggaactctcg gttcgcatgg atgacaagaa agtccgaaga aacgattact 2460

ccatggaatt ttgaggaagt tgtcgataaa ggtgcgtcag ctcaatcgtt catcgagagg 2520

atgaccaact ttgacaagaa tttaccgaac gaaaaagtat tgcctaagca cagtttactt 2580

tacgagtatt tcacagtgta caatgaactc acgaaagtta agtatgtcac tgagggcatg 2640

cgtaaacccg cctttctaag cggagaacag aagaaagcaa tagtagatct gttattcaag 2700

accaaccgca aagtgacagt taagcaattg aaagaggact actttaagaa aattgaatgc 2760

ttcgattctg tcgagatctc cggggtagaa gatcgattta atgcgtcact tggtacgtat 2820

catgacctcc taaagataat taaagataag gacttcctgg ataacgaaga gaatgaagat 2880

atcttagaag atatagtgtt gactcttacc ctctttgaag atcgggaaat gattgaggaa 2940

agactaaaaa catacgctca cctgttcgac gataaggtta tgaaacagtt aaagaggcgt 3000

cgctatacgg gctggggacg attgtcgcgg aaacttatca acgggataag agacaagcaa 3060

agtggtaaaa ctattctcga ttttctaaag agcgacggct tcgccaatag gaactttatg 3120

cagctgatcc atgatgactc tttaaccttc aaagaggata tacaaaaggc acaggtttcc 3180

ggacaagggg actcattgca cgaacatatt gcgaatcttg ctggttcgcc agccatcaaa 3240

aagggcatac tccagacagt caaagtagtg gatgagctag ttaaggtcat gggacgtcac 3300

aaaccggaaa acattgtaat cgagatggca cgcgaaaatc aaacgactca gaaggggcaa 3360

aaaaacagtc gagagcggat gaagagaata gaagagggta ttaaagaact gggcagccag 3420

atcttaaagg agcatcctgt ggaaaatacc caattgcaga acgagaaact ttacctctat 3480

tacctacaaa atggaaggga catgtatgtt gatcaggaac tggacataaa ccgtttatct 3540

gattacgacg tcgatcacat tgtaccccaa tcctttttga aggacgattc aatcgacaat 3600

aaagtgctta cacgctcgga taagaaccga gggaaaagtg acaatgttcc aagcgaggaa 3660

gtcgtaaaga aaatgaagaa ctattggcgg cagctcctaa atgcgaaact gataacgcaa 3720

agaaagttcg ataacttaac taaagctgag aggggtggct tgtctgaact tgacaaggcc 3780

ggatttatta aacgtcagct cgtggaaacc cgccaaatca caaagcatgt tgcacagata 3840

ctagattccc gaatgaatac gaaatacgac gagaacgata agctgattcg ggaagtcaaa 3900

gtaatcactt taaagtcaaa attggtgtcg gacttcagaa aggattttca attctataaa 3960

gttagggaga taaataacta ccaccatgcg cacgacgctt atcttaatgc cgtcgtaggg 4020

accgcactca ttaagaaata cccgaagcta gaaagtgagt ttgtgtatgg tgattacaaa 4080

gtttatgacg tccgtaagat gatcgcgaaa agcgaacagg agataggcaa ggctacagcc 4140

aaatacttct tttattctaa cattatgaat ttctttaaga cggaaatcac tctggcaaac 4200

ggagagatac gcaaacgacc tttaattgaa accaatgggg agacaggtga aatcgtatgg 4260

gataagggcc gggacttcgc gacggtgaga aaagttttgt ccatgcccca agtcaacata 4320

gtaaagaaaa ctgaggtgca gaccggaggg ttttcaaagg aatcgattct tccaaaaagg 4380

aatagtgata agctcatcgc tcgtaaaaag gactgggacc cgaaaaagta cggtggcttc 4440

gatagcccta cagttgccta ttctgtccta gtagtggcaa aagttgagaa gggaaaatcc 4500

aagaaactga agtcagtcaa agaattattg gggataacga ttatggagcg ctcgtctttt 4560

gaaaagaacc ccatcgactt ccttgaggcg aaaggttaca aggaagtaaa aaaggatctc 4620

ataattaaac taccaaagta tagtctgttt gagttagaaa atggccgaaa acggatgttg 4680

gctagcgccg gagagcttca aaaggggaac gaactcgcac taccgtctaa atacgtgaat 4740

ttcctgtatt tagcgtccca ttacgagaag ttgaaaggtt cacctgaaga taacgaacag 4800

aagcaacttt ttgttgagca gcacaaacat tatctcgacg aaatcataga gcaaatttcg 4860

gaattcagta agagagtcat cctagctgat gccaatctgg acaaagtatt aagcgcatac 4920

aacaagcaca gggataaacc catacgtgag caggcggaaa atattatcca tttgtttact 4980

cttaccaacc tcggcgctcc agccgcattc aagtattttg acacaacgat agatcgcaaa 5040

cgatacactt ctaccaagga ggtgctagac gcgacactga ttcaccaatc catcacggga 5100

ttatatgaaa ctcggataga tttgtcacag cttgggggtg actctggtgg ttctactaat 5160

ctgtcagata ttattgaaaa ggagaccggt aagcaactgg ttatccagga atccatcctc 5220

atgctcccag aggaggtgga agaagtcatt gggaacaagc cggaaagcga tatactcgtg 5280

cacaccgcct acgacgagag caccgacgag aatgtcatgc ttctgactag cgacgcccct 5340

gaatacaagc cttgggctct ggtcatacag gatagcaacg gtgagaacaa gattaagatg 5400

ctctctggtg gttctcccaa gaagaagagg aaagtctaac cggtcatcat caccatcacc 5460

attgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg 5520

tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct 5580

aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg 5640

gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg 5700

cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctcgata ccgtcgacct 5760

ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 5820

tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctag ggtgcctaat 5880

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 5940

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 6000

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 6060

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 6120

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 6180

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 6240

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 6300

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 6360

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 6420

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 6480

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 6540

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 6600

ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc 6660

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 6720

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 6780

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 6840

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 6900

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 6960

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 7020

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 7080

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 7140

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 7200

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 7260

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 7320

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 7380

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 7440

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 7500

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 7560

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 7620

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 7680

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 7740

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 7800

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 7860

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 7920

cccgaaaagt gccacctgac gtcgacggat cgggagatcg atctcccgat cccctagggt 7980

cgactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 8040

gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 8100

tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 8160

tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 8220

cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 8280

gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 8340

ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 8400

ctgcccactt ggcagtacat caagtgtatc 8430

<210> 2

<211> 8409

<212> DNA

<213> Artificial Sequence

<220>

<223> C-198-BE3

<400> 2

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat ggatccaaag 420

acattcactt tcaactttaa caatgaacct tgggtcagag gacggcatga gacttacctg 480

tgttatgagg tggagcgcat gcacaatgac acctgggtcc tgctgaacca gcgcaggggc 540

tttctatgca accaggctcc acataaacac ggtttccttg aaggccgcca tgcagagctg 600

tgcttcctgg acgtgattcc cttttggaag ctggacctgg accaggacta cagggttacc 660

tgcttcacct cctggagccc ctgcttcagc tgtgcccagg aaatggctaa attcatttca 720

aaaaacaaac acgtgagcct gtgcatcttc actgcccgca tctatgatga tcaaggaaga 780

tgtcaggagg ggctgcgcac cctggccgag gctggggcca aaatttcaat aatgacatac 840

agtgaattta agcactgctg ggacaccttt gtggaccacc agggatgtcc cttccagccc 900

tgggatggac tagatgagca cagccaagac ctgagtggga ggctgcgggc cattctccag 960

aatcaggaaa acagcggcag cgagactccc gggacctcag agtccgccac acccgaaagt 1020

gataaaaagt attctattgg tttagccatc ggcactaatt ccgttggatg ggctgtcata 1080

accgatgaat acaaagtacc ttcaaagaaa tttaaggtgt tggggaacac agaccgtcat 1140

tcgattaaaa agaatcttat cggtgccctc ctattcgata gtggcgaaac ggcagaggcg 1200

actcgcctga aacgaaccgc tcggagaagg tatacacgtc gcaagaaccg aatatgttac 1260

ttacaagaaa tttttagcaa tgagatggcc aaagttgacg attctttctt tcaccgtttg 1320

gaagagtcct tccttgtcga agaggacaag aaacatgaac ggcaccccat ctttggaaac 1380

atagtagatg aggtggcata tcatgaaaag tacccaacga tttatcacct cagaaaaaag 1440

ctagttgact caactgataa agcggacctg aggttaatct acttggctct tgcccatatg 1500

ataaagttcc gtgggcactt tctcattgag ggtgatctaa atccggacaa ctcggatgtc 1560

gacaaactgt tcatccagtt agtacaaacc tataatcagt tgtttgaaga gaaccctata 1620

aatgcaagtg gcgtggatgc gaaggctatt cttagcgccc gcctctctaa atcccgacgg 1680

ctagaaaacc tgatcgcaca attacccgga gagaagaaaa atgggttgtt cggtaacctt 1740

atagcgctct cactaggcct gacaccaaat tttaagtcga acttcgactt agctgaagat 1800

gccaaattgc agcttagtaa ggacacgtac gatgacgatc tcgacaatct actggcacaa 1860

attggagatc agtatgcgga cttatttttg gctgccaaaa accttagcga tgcaatcctc 1920

ctatctgaca tactgagagt taatactgag attaccaagg cgccgttatc cgcttcaatg 1980

atcaaaaggt acgatgaaca tcaccaagac ttgacacttc tcaaggccct agtccgtcag 2040

caactgcctg agaaatataa ggaaatattc tttgatcagt cgaaaaacgg gtacgcaggt 2100

tatattgacg gcggagcgag tcaagaggaa ttctacaagt ttatcaaacc catattagag 2160

aagatggatg ggacggaaga gttgcttgta aaactcaatc gcgaagatct actgcgaaag 2220

cagcggactt tcgacaacgg tagcattcca catcaaatcc acttaggcga attgcatgct 2280

atacttagaa ggcaggagga tttttatccg ttcctcaaag acaatcgtga aaagattgag 2340

aaaatcctaa cctttcgcat accttactat gtgggacccc tggcccgagg gaactctcgg 2400

ttcgcatgga tgacaagaaa gtccgaagaa acgattactc catggaattt tgaggaagtt 2460

gtcgataaag gtgcgtcagc tcaatcgttc atcgagagga tgaccaactt tgacaagaat 2520

ttaccgaacg aaaaagtatt gcctaagcac agtttacttt acgagtattt cacagtgtac 2580

aatgaactca cgaaagttaa gtatgtcact gagggcatgc gtaaacccgc ctttctaagc 2640

ggagaacaga agaaagcaat agtagatctg ttattcaaga ccaaccgcaa agtgacagtt 2700

aagcaattga aagaggacta ctttaagaaa attgaatgct tcgattctgt cgagatctcc 2760

ggggtagaag atcgatttaa tgcgtcactt ggtacgtatc atgacctcct aaagataatt 2820

aaagataagg acttcctgga taacgaagag aatgaagata tcttagaaga tatagtgttg 2880

actcttaccc tctttgaaga tcgggaaatg attgaggaaa gactaaaaac atacgctcac 2940

ctgttcgacg ataaggttat gaaacagtta aagaggcgtc gctatacggg ctggggacga 3000

ttgtcgcgga aacttatcaa cgggataaga gacaagcaaa gtggtaaaac tattctcgat 3060

tttctaaaga gcgacggctt cgccaatagg aactttatgc agctgatcca tgatgactct 3120

ttaaccttca aagaggatat acaaaaggca caggtttccg gacaagggga ctcattgcac 3180

gaacatattg cgaatcttgc tggttcgcca gccatcaaaa agggcatact ccagacagtc 3240

aaagtagtgg atgagctagt taaggtcatg ggacgtcaca aaccggaaaa cattgtaatc 3300

gagatggcac gcgaaaatca aacgactcag aaggggcaaa aaaacagtcg agagcggatg 3360

aagagaatag aagagggtat taaagaactg ggcagccaga tcttaaagga gcatcctgtg 3420

gaaaataccc aattgcagaa cgagaaactt tacctctatt acctacaaaa tggaagggac 3480

atgtatgttg atcaggaact ggacataaac cgtttatctg attacgacgt cgatcacatt 3540

gtaccccaat cctttttgaa ggacgattca atcgacaata aagtgcttac acgctcggat 3600

aagaaccgag ggaaaagtga caatgttcca agcgaggaag tcgtaaagaa aatgaagaac 3660

tattggcggc agctcctaaa tgcgaaactg ataacgcaaa gaaagttcga taacttaact 3720

aaagctgaga ggggtggctt gtctgaactt gacaaggccg gatttattaa acgtcagctc 3780

gtggaaaccc gccaaatcac aaagcatgtt gcacagatac tagattcccg aatgaatacg 3840

aaatacgacg agaacgataa gctgattcgg gaagtcaaag taatcacttt aaagtcaaaa 3900

ttggtgtcgg acttcagaaa ggattttcaa ttctataaag ttagggagat aaataactac 3960

caccatgcgc acgacgctta tcttaatgcc gtcgtaggga ccgcactcat taagaaatac 4020

ccgaagctag aaagtgagtt tgtgtatggt gattacaaag tttatgacgt ccgtaagatg 4080

atcgcgaaaa gcgaacagga gataggcaag gctacagcca aatacttctt ttattctaac 4140

attatgaatt tctttaagac ggaaatcact ctggcaaacg gagagatacg caaacgacct 4200

ttaattgaaa ccaatgggga gacaggtgaa atcgtatggg ataagggccg ggacttcgcg 4260

acggtgagaa aagttttgtc catgccccaa gtcaacatag taaagaaaac tgaggtgcag 4320

accggagggt tttcaaagga atcgattctt ccaaaaagga atagtgataa gctcatcgct 4380

cgtaaaaagg actgggaccc gaaaaagtac ggtggcttcg atagccctac agttgcctat 4440

tctgtcctag tagtggcaaa agttgagaag ggaaaatcca agaaactgaa gtcagtcaaa 4500

gaattattgg ggataacgat tatggagcgc tcgtcttttg aaaagaaccc catcgacttc 4560

cttgaggcga aaggttacaa ggaagtaaaa aaggatctca taattaaact accaaagtat 4620

agtctgtttg agttagaaaa tggccgaaaa cggatgttgg ctagcgccgg agagcttcaa 4680

aaggggaacg aactcgcact accgtctaaa tacgtgaatt tcctgtattt agcgtcccat 4740

tacgagaagt tgaaaggttc acctgaagat aacgaacaga agcaactttt tgttgagcag 4800

cacaaacatt atctcgacga aatcatagag caaatttcgg aattcagtaa gagagtcatc 4860

ctagctgatg ccaatctgga caaagtatta agcgcataca acaagcacag ggataaaccc 4920

atacgtgagc aggcggaaaa tattatccat ttgtttactc ttaccaacct cggcgctcca 4980

gccgcattca agtattttga cacaacgata gatcgcaaac gatacacttc taccaaggag 5040

gtgctagacg cgacactgat tcaccaatcc atcacgggat tatatgaaac tcggatagat 5100

ttgtcacagc ttgggggtga ctctggtggt tctactaatc tgtcagatat tattgaaaag 5160

gagaccggta agcaactggt tatccaggaa tccatcctca tgctcccaga ggaggtggaa 5220

gaagtcattg ggaacaagcc ggaaagcgat atactcgtgc acaccgccta cgacgagagc 5280

accgacgaga atgtcatgct tctgactagc gacgcccctg aatacaagcc ttgggctctg 5340

gtcatacagg atagcaacgg tgagaacaag attaagatgc tctctggtgg ttctcccaag 5400

aagaagagga aagtctaacc ggtcatcatc accatcacca ttgagtttaa acccgctgat 5460

cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt 5520

ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat 5580

cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg 5640

gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg 5700

aggcggaaag aaccagctgg ggctcgatac cgtcgacctc tagctagagc ttggcgtaat 5760

catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 5820

gagccggaag cataaagtgt aaagcctagg gtgcctaatg agtgagctaa ctcacattaa 5880

ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 5940

gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 6000

tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 6060

cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 6120

gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 6180

gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 6240

gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 6300

ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 6360

atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 6420

tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 6480

ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 6540

gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 6600

ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 6660

ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 6720

agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 6780

ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 6840

aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 6900

tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 6960

cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 7020

tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 7080

cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 7140

ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 7200

gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 7260

gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 7320

gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 7380

gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 7440

tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 7500

aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 7560

cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 7620

caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 7680

cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 7740

ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 7800

aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 7860

tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 7920

tcgacggatc gggagatcga tctcccgatc ccctagggtc gactctcagt acaatctgct 7980

ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt 8040

agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga 8100

atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt 8160

gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 8220

catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 8280

acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 8340

ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 8400

aagtgtatc 8409

<210> 3

<211> 8997

<212> DNA

<213> Artificial Sequence

<220>

<223> 4M-BE3

<400> 3

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaagcctcac 420

ttcagaaaca cagtggagcg aatgtatcga gacacattct cctacaactt ttataatgca 480

cccatccttt ctcgtcggaa taccgtctgg ctgtgctacg aagtgaaaac aaagggtccc 540

tcaaggcccc ctttggacgc aaagatcttt cgaggccagg tgtattccga acttaagtac 600

cacccagaga tgagattctt ccactggttc agcaagtgga ggaagctgca tcgtgaccag 660

gagtatgagg tcacctggta catatccttg agcccctgca caaagtgtac aagggatatg 720

gccacgttcc tggccgagga cccgaaggtt accctgacca tctttgttgc ccgcctcgcc 780

tacttccttg acccagatta ccaggaggcg cttcgcagcc tgtgtcagaa aagagacggt 840

ccgcgtgcca ccatgaagat catgaattat gacgaatttc agcactgttg gagcaagttc 900

gtgtacagcc aaagagagct atttgagcct tggaataatc tgcctaaata ttatatatta 960

ctgcacatca tgctggggga gattctcaga cactcgatgg atccacccac attcactttc 1020

aactttaaca atgaaccttg ggtcagagga cggcatgaga cttacctgtg ttatgaggtg 1080

gagcgcatgc acaatgacac ctgggtcctg ctgaaccagc gcaggggctt tctatgcaac 1140

caggctccac ataaacacgg tttccttgaa ggccgccatg cagagctgtg cttcctggac 1200

gtgattccct tttggaagct ggacctggac caggactaca gggttacctg cttcacctcc 1260

tggagcccct gcttcagctg tgcccaggaa atggctaaat tcatttcaaa aaacaaacac 1320

gtgagcctgt gcatcttcac tgcccgcatc tatgatgatc aaggaagatg tcaggagggg 1380

ctgcgcaccc tggccgaggc tggggccaaa atttcaataa tgacatacag tgaatttaag 1440

cactgctggg acacctttgt ggaccaccag ggatgtccct tccagccctg ggatggacta 1500

gatgagcaca gccaagacct gagtgggagg ctgcgggcca ttctccagaa tcaggaaaac 1560

agcggcagcg agactcccgg gacctcagag tccgccacac ccgaaagtga taaaaagtat 1620

tctattggtt tagccatcgg cactaattcc gttggatggg ctgtcataac cgatgaatac 1680

aaagtacctt caaagaaatt taaggtgttg gggaacacag accgtcattc gattaaaaag 1740

aatcttatcg gtgccctcct attcgatagt ggcgaaacgg cagaggcgac tcgcctgaaa 1800

cgaaccgctc ggagaaggta tacacgtcgc aagaaccgaa tatgttactt acaagaaatt 1860

tttagcaatg agatggccaa agttgacgat tctttctttc accgtttgga agagtccttc 1920

cttgtcgaag aggacaagaa acatgaacgg caccccatct ttggaaacat agtagatgag 1980

gtggcatatc atgaaaagta cccaacgatt tatcacctca gaaaaaagct agttgactca 2040

actgataaag cggacctgag gttaatctac ttggctcttg cccatatgat aaagttccgt 2100

gggcactttc tcattgaggg tgatctaaat ccggacaact cggatgtcga caaactgttc 2160

atccagttag tacaaaccta taatcagttg tttgaagaga accctataaa tgcaagtggc 2220

gtggatgcga aggctattct tagcgcccgc ctctctaaat cccgacggct agaaaacctg 2280

atcgcacaat tacccggaga gaagaaaaat gggttgttcg gtaaccttat agcgctctca 2340

ctaggcctga caccaaattt taagtcgaac ttcgacttag ctgaagatgc caaattgcag 2400

cttagtaagg acacgtacga tgacgatctc gacaatctac tggcacaaat tggagatcag 2460

tatgcggact tatttttggc tgccaaaaac cttagcgatg caatcctcct atctgacata 2520

ctgagagtta atactgagat taccaaggcg ccgttatccg cttcaatgat caaaaggtac 2580

gatgaacatc accaagactt gacacttctc aaggccctag tccgtcagca actgcctgag 2640

aaatataagg aaatattctt tgatcagtcg aaaaacgggt acgcaggtta tattgacggc 2700

ggagcgagtc aagaggaatt ctacaagttt atcaaaccca tattagagaa gatggatggg 2760

acggaagagt tgcttgtaaa actcaatcgc gaagatctac tgcgaaagca gcggactttc 2820

gacaacggta gcattccaca tcaaatccac ttaggcgaat tgcatgctat acttagaagg 2880

caggaggatt tttatccgtt cctcaaagac aatcgtgaaa agattgagaa aatcctaacc 2940

tttcgcatac cttactatgt gggacccctg gcccgaggga actctcggtt cgcatggatg 3000

acaagaaagt ccgaagaaac gattactcca tggaattttg aggaagttgt cgataaaggt 3060

gcgtcagctc aatcgttcat cgagaggatg accaactttg acaagaattt accgaacgaa 3120

aaagtattgc ctaagcacag tttactttac gagtatttca cagtgtacaa tgaactcacg 3180

aaagttaagt atgtcactga gggcatgcgt aaacccgcct ttctaagcgg agaacagaag 3240

aaagcaatag tagatctgtt attcaagacc aaccgcaaag tgacagttaa gcaattgaaa 3300

gaggactact ttaagaaaat tgaatgcttc gattctgtcg agatctccgg ggtagaagat 3360

cgatttaatg cgtcacttgg tacgtatcat gacctcctaa agataattaa agataaggac 3420

ttcctggata acgaagagaa tgaagatatc ttagaagata tagtgttgac tcttaccctc 3480

tttgaagatc gggaaatgat tgaggaaaga ctaaaaacat acgctcacct gttcgacgat 3540

aaggttatga aacagttaaa gaggcgtcgc tatacgggct ggggacgatt gtcgcggaaa 3600

cttatcaacg ggataagaga caagcaaagt ggtaaaacta ttctcgattt tctaaagagc 3660

gacggcttcg ccaataggaa ctttatgcag ctgatccatg atgactcttt aaccttcaaa 3720

gaggatatac aaaaggcaca ggtttccgga caaggggact cattgcacga acatattgcg 3780

aatcttgctg gttcgccagc catcaaaaag ggcatactcc agacagtcaa agtagtggat 3840

gagctagtta aggtcatggg acgtcacaaa ccggaaaaca ttgtaatcga gatggcacgc 3900

gaaaatcaaa cgactcagaa ggggcaaaaa aacagtcgag agcggatgaa gagaatagaa 3960

gagggtatta aagaactggg cagccagatc ttaaaggagc atcctgtgga aaatacccaa 4020

ttgcagaacg agaaacttta cctctattac ctacaaaatg gaagggacat gtatgttgat 4080

caggaactgg acataaaccg tttatctgat tacgacgtcg atcacattgt accccaatcc 4140

tttttgaagg acgattcaat cgacaataaa gtgcttacac gctcggataa gaaccgaggg 4200

aaaagtgaca atgttccaag cgaggaagtc gtaaagaaaa tgaagaacta ttggcggcag 4260

ctcctaaatg cgaaactgat aacgcaaaga aagttcgata acttaactaa agctgagagg 4320

ggtggcttgt ctgaacttga caaggccgga tttattaaac gtcagctcgt ggaaacccgc 4380

caaatcacaa agcatgttgc acagatacta gattcccgaa tgaatacgaa atacgacgag 4440

aacgataagc tgattcggga agtcaaagta atcactttaa agtcaaaatt ggtgtcggac 4500

ttcagaaagg attttcaatt ctataaagtt agggagataa ataactacca ccatgcgcac 4560

gacgcttatc ttaatgccgt cgtagggacc gcactcatta agaaataccc gaagctagaa 4620

agtgagtttg tgtatggtga ttacaaagtt tatgacgtcc gtaagatgat cgcgaaaagc 4680

gaacaggaga taggcaaggc tacagccaaa tacttctttt attctaacat tatgaatttc 4740

tttaagacgg aaatcactct ggcaaacgga gagatacgca aacgaccttt aattgaaacc 4800

aatggggaga caggtgaaat cgtatgggat aagggccggg acttcgcgac ggtgagaaaa 4860

gttttgtcca tgccccaagt caacatagta aagaaaactg aggtgcagac cggagggttt 4920

tcaaaggaat cgattcttcc aaaaaggaat agtgataagc tcatcgctcg taaaaaggac 4980

tgggacccga aaaagtacgg tggcttcgat agccctacag ttgcctattc tgtcctagta 5040

gtggcaaaag ttgagaaggg aaaatccaag aaactgaagt cagtcaaaga attattgggg 5100

ataacgatta tggagcgctc gtcttttgaa aagaacccca tcgacttcct tgaggcgaaa 5160

ggttacaagg aagtaaaaaa ggatctcata attaaactac caaagtatag tctgtttgag 5220

ttagaaaatg gccgaaaacg gatgttggct agcgccggag agcttcaaaa ggggaacgaa 5280

ctcgcactac cgtctaaata cgtgaatttc ctgtatttag cgtcccatta cgagaagttg 5340

aaaggttcac ctgaagataa cgaacagaag caactttttg ttgagcagca caaacattat 5400

ctcgacgaaa tcatagagca aatttcggaa ttcagtaaga gagtcatcct agctgatgcc 5460

aatctggaca aagtattaag cgcatacaac aagcacaggg ataaacccat acgtgagcag 5520

gcggaaaata ttatccattt gtttactctt accaacctcg gcgctccagc cgcattcaag 5580

tattttgaca caacgataga tcgcaaacga tacacttcta ccaaggaggt gctagacgcg 5640

acactgattc accaatccat cacgggatta tatgaaactc ggatagattt gtcacagctt 5700

gggggtgact ctggtggttc tactaatctg tcagatatta ttgaaaagga gaccggtaag 5760

caactggtta tccaggaatc catcctcatg ctcccagagg aggtggaaga agtcattggg 5820

aacaagccgg aaagcgatat actcgtgcac accgcctacg acgagagcac cgacgagaat 5880

gtcatgcttc tgactagcga cgcccctgaa tacaagcctt gggctctggt catacaggat 5940

agcaacggtg agaacaagat taagatgctc tctggtggtt ctcccaagaa gaagaggaaa 6000

gtctaaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg 6060

tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg 6120

aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga 6180

gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg 6240

aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa 6300

ccagctgggg ctcgataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc 6360

tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 6420

taaagtgtaa agcctagggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 6480

cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 6540

gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 6600

tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 6660

tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 6720

ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 6780

agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 6840

accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 6900

ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 6960

gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 7020

ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 7080

gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 7140

taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 7200

tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 7260

gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 7320

cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 7380

agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 7440

cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 7500

cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 7560

ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 7620

taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 7680

tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 7740

ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 7800

atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 7860

gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 7920

tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 7980

cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 8040

taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 8100

ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 8160

ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 8220

cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 8280

ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 8340

gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 8400

gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 8460

aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg 8520

gagatcgatc tcccgatccc ctagggtcga ctctcagtac aatctgctct gatgccgcat 8580

agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca 8640

aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg 8700

ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat 8760

tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt 8820

tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 8880

cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 8940

gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatc 8997

<210> 4

<211> 8997

<212> DNA

<213> Artificial Sequence

<220>

<223> 4M+D128K-BE3

<400> 4

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaagcctcac 420

ttcagaaaca cagtggagcg aatgtatcga gacacattct cctacaactt ttataatgca 480

cccatccttt ctcgtcggaa taccgtctgg ctgtgctacg aagtgaaaac aaagggtccc 540

tcaaggcccc ctttggacgc aaagatcttt cgaggccagg tgtattccga acttaagtac 600

cacccagaga tgagattctt ccactggttc agcaagtgga ggaagctgca tcgtgaccag 660

gagtatgagg tcacctggta catatccttg agcccctgca caaagtgtac aagggatatg 720

gccacgttcc tggccgagga cccgaaggtt accctgacca tctttgttgc ccgcctcgcc 780

tacttcctta agccagatta ccaggaggcg cttcgcagcc tgtgtcagaa aagagacggt 840

ccgcgtgcca ccatgaagat catgaattat gacgaatttc agcactgttg gagcaagttc 900

gtgtacagcc aaagagagct atttgagcct tggaataatc tgcctaaata ttatatatta 960

ctgcacatca tgctggggga gattctcaga cactcgatgg atccacccac attcactttc 1020

aactttaaca atgaaccttg ggtcagagga cggcatgaga cttacctgtg ttatgaggtg 1080

gagcgcatgc acaatgacac ctgggtcctg ctgaaccagc gcaggggctt tctatgcaac 1140

caggctccac ataaacacgg tttccttgaa ggccgccatg cagagctgtg cttcctggac 1200

gtgattccct tttggaagct ggacctggac caggactaca gggttacctg cttcacctcc 1260

tggagcccct gcttcagctg tgcccaggaa atggctaaat tcatttcaaa aaacaaacac 1320

gtgagcctgt gcatcttcac tgcccgcatc tatgatgatc aaggaagatg tcaggagggg 1380

ctgcgcaccc tggccgaggc tggggccaaa atttcaataa tgacatacag tgaatttaag 1440

cactgctggg acacctttgt ggaccaccag ggatgtccct tccagccctg ggatggacta 1500

gatgagcaca gccaagacct gagtgggagg ctgcgggcca ttctccagaa tcaggaaaac 1560

agcggcagcg agactcccgg gacctcagag tccgccacac ccgaaagtga taaaaagtat 1620

tctattggtt tagccatcgg cactaattcc gttggatggg ctgtcataac cgatgaatac 1680

aaagtacctt caaagaaatt taaggtgttg gggaacacag accgtcattc gattaaaaag 1740

aatcttatcg gtgccctcct attcgatagt ggcgaaacgg cagaggcgac tcgcctgaaa 1800

cgaaccgctc ggagaaggta tacacgtcgc aagaaccgaa tatgttactt acaagaaatt 1860

tttagcaatg agatggccaa agttgacgat tctttctttc accgtttgga agagtccttc 1920

cttgtcgaag aggacaagaa acatgaacgg caccccatct ttggaaacat agtagatgag 1980

gtggcatatc atgaaaagta cccaacgatt tatcacctca gaaaaaagct agttgactca 2040

actgataaag cggacctgag gttaatctac ttggctcttg cccatatgat aaagttccgt 2100

gggcactttc tcattgaggg tgatctaaat ccggacaact cggatgtcga caaactgttc 2160

atccagttag tacaaaccta taatcagttg tttgaagaga accctataaa tgcaagtggc 2220

gtggatgcga aggctattct tagcgcccgc ctctctaaat cccgacggct agaaaacctg 2280

atcgcacaat tacccggaga gaagaaaaat gggttgttcg gtaaccttat agcgctctca 2340

ctaggcctga caccaaattt taagtcgaac ttcgacttag ctgaagatgc caaattgcag 2400

cttagtaagg acacgtacga tgacgatctc gacaatctac tggcacaaat tggagatcag 2460

tatgcggact tatttttggc tgccaaaaac cttagcgatg caatcctcct atctgacata 2520

ctgagagtta atactgagat taccaaggcg ccgttatccg cttcaatgat caaaaggtac 2580

gatgaacatc accaagactt gacacttctc aaggccctag tccgtcagca actgcctgag 2640

aaatataagg aaatattctt tgatcagtcg aaaaacgggt acgcaggtta tattgacggc 2700

ggagcgagtc aagaggaatt ctacaagttt atcaaaccca tattagagaa gatggatggg 2760

acggaagagt tgcttgtaaa actcaatcgc gaagatctac tgcgaaagca gcggactttc 2820

gacaacggta gcattccaca tcaaatccac ttaggcgaat tgcatgctat acttagaagg 2880

caggaggatt tttatccgtt cctcaaagac aatcgtgaaa agattgagaa aatcctaacc 2940

tttcgcatac cttactatgt gggacccctg gcccgaggga actctcggtt cgcatggatg 3000

acaagaaagt ccgaagaaac gattactcca tggaattttg aggaagttgt cgataaaggt 3060

gcgtcagctc aatcgttcat cgagaggatg accaactttg acaagaattt accgaacgaa 3120

aaagtattgc ctaagcacag tttactttac gagtatttca cagtgtacaa tgaactcacg 3180

aaagttaagt atgtcactga gggcatgcgt aaacccgcct ttctaagcgg agaacagaag 3240

aaagcaatag tagatctgtt attcaagacc aaccgcaaag tgacagttaa gcaattgaaa 3300

gaggactact ttaagaaaat tgaatgcttc gattctgtcg agatctccgg ggtagaagat 3360

cgatttaatg cgtcacttgg tacgtatcat gacctcctaa agataattaa agataaggac 3420

ttcctggata acgaagagaa tgaagatatc ttagaagata tagtgttgac tcttaccctc 3480

tttgaagatc gggaaatgat tgaggaaaga ctaaaaacat acgctcacct gttcgacgat 3540

aaggttatga aacagttaaa gaggcgtcgc tatacgggct ggggacgatt gtcgcggaaa 3600

cttatcaacg ggataagaga caagcaaagt ggtaaaacta ttctcgattt tctaaagagc 3660

gacggcttcg ccaataggaa ctttatgcag ctgatccatg atgactcttt aaccttcaaa 3720

gaggatatac aaaaggcaca ggtttccgga caaggggact cattgcacga acatattgcg 3780

aatcttgctg gttcgccagc catcaaaaag ggcatactcc agacagtcaa agtagtggat 3840

gagctagtta aggtcatggg acgtcacaaa ccggaaaaca ttgtaatcga gatggcacgc 3900

gaaaatcaaa cgactcagaa ggggcaaaaa aacagtcgag agcggatgaa gagaatagaa 3960

gagggtatta aagaactggg cagccagatc ttaaaggagc atcctgtgga aaatacccaa 4020

ttgcagaacg agaaacttta cctctattac ctacaaaatg gaagggacat gtatgttgat 4080

caggaactgg acataaaccg tttatctgat tacgacgtcg atcacattgt accccaatcc 4140

tttttgaagg acgattcaat cgacaataaa gtgcttacac gctcggataa gaaccgaggg 4200

aaaagtgaca atgttccaag cgaggaagtc gtaaagaaaa tgaagaacta ttggcggcag 4260

ctcctaaatg cgaaactgat aacgcaaaga aagttcgata acttaactaa agctgagagg 4320

ggtggcttgt ctgaacttga caaggccgga tttattaaac gtcagctcgt ggaaacccgc 4380

caaatcacaa agcatgttgc acagatacta gattcccgaa tgaatacgaa atacgacgag 4440

aacgataagc tgattcggga agtcaaagta atcactttaa agtcaaaatt ggtgtcggac 4500

ttcagaaagg attttcaatt ctataaagtt agggagataa ataactacca ccatgcgcac 4560

gacgcttatc ttaatgccgt cgtagggacc gcactcatta agaaataccc gaagctagaa 4620

agtgagtttg tgtatggtga ttacaaagtt tatgacgtcc gtaagatgat cgcgaaaagc 4680

gaacaggaga taggcaaggc tacagccaaa tacttctttt attctaacat tatgaatttc 4740

tttaagacgg aaatcactct ggcaaacgga gagatacgca aacgaccttt aattgaaacc 4800

aatggggaga caggtgaaat cgtatgggat aagggccggg acttcgcgac ggtgagaaaa 4860

gttttgtcca tgccccaagt caacatagta aagaaaactg aggtgcagac cggagggttt 4920

tcaaaggaat cgattcttcc aaaaaggaat agtgataagc tcatcgctcg taaaaaggac 4980

tgggacccga aaaagtacgg tggcttcgat agccctacag ttgcctattc tgtcctagta 5040

gtggcaaaag ttgagaaggg aaaatccaag aaactgaagt cagtcaaaga attattgggg 5100

ataacgatta tggagcgctc gtcttttgaa aagaacccca tcgacttcct tgaggcgaaa 5160

ggttacaagg aagtaaaaaa ggatctcata attaaactac caaagtatag tctgtttgag 5220

ttagaaaatg gccgaaaacg gatgttggct agcgccggag agcttcaaaa ggggaacgaa 5280

ctcgcactac cgtctaaata cgtgaatttc ctgtatttag cgtcccatta cgagaagttg 5340

aaaggttcac ctgaagataa cgaacagaag caactttttg ttgagcagca caaacattat 5400

ctcgacgaaa tcatagagca aatttcggaa ttcagtaaga gagtcatcct agctgatgcc 5460

aatctggaca aagtattaag cgcatacaac aagcacaggg ataaacccat acgtgagcag 5520

gcggaaaata ttatccattt gtttactctt accaacctcg gcgctccagc cgcattcaag 5580

tattttgaca caacgataga tcgcaaacga tacacttcta ccaaggaggt gctagacgcg 5640

acactgattc accaatccat cacgggatta tatgaaactc ggatagattt gtcacagctt 5700

gggggtgact ctggtggttc tactaatctg tcagatatta ttgaaaagga gaccggtaag 5760

caactggtta tccaggaatc catcctcatg ctcccagagg aggtggaaga agtcattggg 5820

aacaagccgg aaagcgatat actcgtgcac accgcctacg acgagagcac cgacgagaat 5880

gtcatgcttc tgactagcga cgcccctgaa tacaagcctt gggctctggt catacaggat 5940

agcaacggtg agaacaagat taagatgctc tctggtggtt ctcccaagaa gaagaggaaa 6000

gtctaaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg 6060

tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg 6120

aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga 6180

gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg 6240

aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa 6300

ccagctgggg ctcgataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc 6360

tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 6420

taaagtgtaa agcctagggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 6480

cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 6540

gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 6600

tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 6660

tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 6720

ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 6780

agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 6840

accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 6900

ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 6960

gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 7020

ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 7080

gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 7140

taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 7200

tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 7260

gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 7320

cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 7380

agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 7440

cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 7500

cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 7560

ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 7620

taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 7680

tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 7740

ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 7800

atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 7860

gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 7920

tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 7980

cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 8040

taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 8100

ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 8160

ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 8220

cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 8280

ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 8340

gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 8400

gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 8460

aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg 8520

gagatcgatc tcccgatccc ctagggtcga ctctcagtac aatctgctct gatgccgcat 8580

agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca 8640

aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg 8700

ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat 8760

tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt 8820

tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 8880

cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 8940

gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatc 8997

<210> 5

<211> 8997

<212> DNA

<213> Artificial Sequence

<220>

<223> 4M+P199A-BE3

<400> 5

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaagcctcac 420

ttcagaaaca cagtggagcg aatgtatcga gacacattct cctacaactt ttataatgca 480

cccatccttt ctcgtcggaa taccgtctgg ctgtgctacg aagtgaaaac aaagggtccc 540

tcaaggcccc ctttggacgc aaagatcttt cgaggccagg tgtattccga acttaagtac 600

cacccagaga tgagattctt ccactggttc agcaagtgga ggaagctgca tcgtgaccag 660

gagtatgagg tcacctggta catatccttg agcccctgca caaagtgtac aagggatatg 720

gccacgttcc tggccgagga cccgaaggtt accctgacca tctttgttgc ccgcctcgcc 780

tacttccttg acccagatta ccaggaggcg cttcgcagcc tgtgtcagaa aagagacggt 840

ccgcgtgcca ccatgaagat catgaattat gacgaatttc agcactgttg gagcaagttc 900

gtgtacagcc aaagagagct atttgagcct tggaataatc tgcctaaata ttatatatta 960

ctgcacatca tgctggggga gattctcaga cactcgatgg atgcccccac attcactttc 1020

aactttaaca atgaaccttg ggtcagagga cggcatgaga cttacctgtg ttatgaggtg 1080

gagcgcatgc acaatgacac ctgggtcctg ctgaaccagc gcaggggctt tctatgcaac 1140

caggctccac ataaacacgg tttccttgaa ggccgccatg cagagctgtg cttcctggac 1200

gtgattccct tttggaagct ggacctggac caggactaca gggttacctg cttcacctcc 1260

tggagcccct gcttcagctg tgcccaggaa atggctaaat tcatttcaaa aaacaaacac 1320

gtgagcctgt gcatcttcac tgcccgcatc tatgatgatc aaggaagatg tcaggagggg 1380

ctgcgcaccc tggccgaggc tggggccaaa atttcaataa tgacatacag tgaatttaag 1440

cactgctggg acacctttgt ggaccaccag ggatgtccct tccagccctg ggatggacta 1500

gatgagcaca gccaagacct gagtgggagg ctgcgggcca ttctccagaa tcaggaaaac 1560

agcggcagcg agactcccgg gacctcagag tccgccacac ccgaaagtga taaaaagtat 1620

tctattggtt tagccatcgg cactaattcc gttggatggg ctgtcataac cgatgaatac 1680

aaagtacctt caaagaaatt taaggtgttg gggaacacag accgtcattc gattaaaaag 1740

aatcttatcg gtgccctcct attcgatagt ggcgaaacgg cagaggcgac tcgcctgaaa 1800

cgaaccgctc ggagaaggta tacacgtcgc aagaaccgaa tatgttactt acaagaaatt 1860

tttagcaatg agatggccaa agttgacgat tctttctttc accgtttgga agagtccttc 1920

cttgtcgaag aggacaagaa acatgaacgg caccccatct ttggaaacat agtagatgag 1980

gtggcatatc atgaaaagta cccaacgatt tatcacctca gaaaaaagct agttgactca 2040

actgataaag cggacctgag gttaatctac ttggctcttg cccatatgat aaagttccgt 2100

gggcactttc tcattgaggg tgatctaaat ccggacaact cggatgtcga caaactgttc 2160

atccagttag tacaaaccta taatcagttg tttgaagaga accctataaa tgcaagtggc 2220

gtggatgcga aggctattct tagcgcccgc ctctctaaat cccgacggct agaaaacctg 2280

atcgcacaat tacccggaga gaagaaaaat gggttgttcg gtaaccttat agcgctctca 2340

ctaggcctga caccaaattt taagtcgaac ttcgacttag ctgaagatgc caaattgcag 2400

cttagtaagg acacgtacga tgacgatctc gacaatctac tggcacaaat tggagatcag 2460

tatgcggact tatttttggc tgccaaaaac cttagcgatg caatcctcct atctgacata 2520

ctgagagtta atactgagat taccaaggcg ccgttatccg cttcaatgat caaaaggtac 2580

gatgaacatc accaagactt gacacttctc aaggccctag tccgtcagca actgcctgag 2640

aaatataagg aaatattctt tgatcagtcg aaaaacgggt acgcaggtta tattgacggc 2700

ggagcgagtc aagaggaatt ctacaagttt atcaaaccca tattagagaa gatggatggg 2760

acggaagagt tgcttgtaaa actcaatcgc gaagatctac tgcgaaagca gcggactttc 2820

gacaacggta gcattccaca tcaaatccac ttaggcgaat tgcatgctat acttagaagg 2880

caggaggatt tttatccgtt cctcaaagac aatcgtgaaa agattgagaa aatcctaacc 2940

tttcgcatac cttactatgt gggacccctg gcccgaggga actctcggtt cgcatggatg 3000

acaagaaagt ccgaagaaac gattactcca tggaattttg aggaagttgt cgataaaggt 3060

gcgtcagctc aatcgttcat cgagaggatg accaactttg acaagaattt accgaacgaa 3120

aaagtattgc ctaagcacag tttactttac gagtatttca cagtgtacaa tgaactcacg 3180

aaagttaagt atgtcactga gggcatgcgt aaacccgcct ttctaagcgg agaacagaag 3240

aaagcaatag tagatctgtt attcaagacc aaccgcaaag tgacagttaa gcaattgaaa 3300

gaggactact ttaagaaaat tgaatgcttc gattctgtcg agatctccgg ggtagaagat 3360

cgatttaatg cgtcacttgg tacgtatcat gacctcctaa agataattaa agataaggac 3420

ttcctggata acgaagagaa tgaagatatc ttagaagata tagtgttgac tcttaccctc 3480

tttgaagatc gggaaatgat tgaggaaaga ctaaaaacat acgctcacct gttcgacgat 3540

aaggttatga aacagttaaa gaggcgtcgc tatacgggct ggggacgatt gtcgcggaaa 3600

cttatcaacg ggataagaga caagcaaagt ggtaaaacta ttctcgattt tctaaagagc 3660

gacggcttcg ccaataggaa ctttatgcag ctgatccatg atgactcttt aaccttcaaa 3720

gaggatatac aaaaggcaca ggtttccgga caaggggact cattgcacga acatattgcg 3780

aatcttgctg gttcgccagc catcaaaaag ggcatactcc agacagtcaa agtagtggat 3840

gagctagtta aggtcatggg acgtcacaaa ccggaaaaca ttgtaatcga gatggcacgc 3900

gaaaatcaaa cgactcagaa ggggcaaaaa aacagtcgag agcggatgaa gagaatagaa 3960

gagggtatta aagaactggg cagccagatc ttaaaggagc atcctgtgga aaatacccaa 4020

ttgcagaacg agaaacttta cctctattac ctacaaaatg gaagggacat gtatgttgat 4080

caggaactgg acataaaccg tttatctgat tacgacgtcg atcacattgt accccaatcc 4140

tttttgaagg acgattcaat cgacaataaa gtgcttacac gctcggataa gaaccgaggg 4200

aaaagtgaca atgttccaag cgaggaagtc gtaaagaaaa tgaagaacta ttggcggcag 4260

ctcctaaatg cgaaactgat aacgcaaaga aagttcgata acttaactaa agctgagagg 4320

ggtggcttgt ctgaacttga caaggccgga tttattaaac gtcagctcgt ggaaacccgc 4380

caaatcacaa agcatgttgc acagatacta gattcccgaa tgaatacgaa atacgacgag 4440

aacgataagc tgattcggga agtcaaagta atcactttaa agtcaaaatt ggtgtcggac 4500

ttcagaaagg attttcaatt ctataaagtt agggagataa ataactacca ccatgcgcac 4560

gacgcttatc ttaatgccgt cgtagggacc gcactcatta agaaataccc gaagctagaa 4620

agtgagtttg tgtatggtga ttacaaagtt tatgacgtcc gtaagatgat cgcgaaaagc 4680

gaacaggaga taggcaaggc tacagccaaa tacttctttt attctaacat tatgaatttc 4740

tttaagacgg aaatcactct ggcaaacgga gagatacgca aacgaccttt aattgaaacc 4800

aatggggaga caggtgaaat cgtatgggat aagggccggg acttcgcgac ggtgagaaaa 4860

gttttgtcca tgccccaagt caacatagta aagaaaactg aggtgcagac cggagggttt 4920

tcaaaggaat cgattcttcc aaaaaggaat agtgataagc tcatcgctcg taaaaaggac 4980

tgggacccga aaaagtacgg tggcttcgat agccctacag ttgcctattc tgtcctagta 5040

gtggcaaaag ttgagaaggg aaaatccaag aaactgaagt cagtcaaaga attattgggg 5100

ataacgatta tggagcgctc gtcttttgaa aagaacccca tcgacttcct tgaggcgaaa 5160

ggttacaagg aagtaaaaaa ggatctcata attaaactac caaagtatag tctgtttgag 5220

ttagaaaatg gccgaaaacg gatgttggct agcgccggag agcttcaaaa ggggaacgaa 5280

ctcgcactac cgtctaaata cgtgaatttc ctgtatttag cgtcccatta cgagaagttg 5340

aaaggttcac ctgaagataa cgaacagaag caactttttg ttgagcagca caaacattat 5400

ctcgacgaaa tcatagagca aatttcggaa ttcagtaaga gagtcatcct agctgatgcc 5460

aatctggaca aagtattaag cgcatacaac aagcacaggg ataaacccat acgtgagcag 5520

gcggaaaata ttatccattt gtttactctt accaacctcg gcgctccagc cgcattcaag 5580

tattttgaca caacgataga tcgcaaacga tacacttcta ccaaggaggt gctagacgcg 5640

acactgattc accaatccat cacgggatta tatgaaactc ggatagattt gtcacagctt 5700

gggggtgact ctggtggttc tactaatctg tcagatatta ttgaaaagga gaccggtaag 5760

caactggtta tccaggaatc catcctcatg ctcccagagg aggtggaaga agtcattggg 5820

aacaagccgg aaagcgatat actcgtgcac accgcctacg acgagagcac cgacgagaat 5880

gtcatgcttc tgactagcga cgcccctgaa tacaagcctt gggctctggt catacaggat 5940

agcaacggtg agaacaagat taagatgctc tctggtggtt ctcccaagaa gaagaggaaa 6000

gtctaaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg 6060

tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg 6120

aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga 6180

gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg 6240

aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa 6300

ccagctgggg ctcgataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc 6360

tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 6420

taaagtgtaa agcctagggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 6480

cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 6540

gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 6600

tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 6660

tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 6720

ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 6780

agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 6840

accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 6900

ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 6960

gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 7020

ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 7080

gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 7140

taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 7200

tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 7260

gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 7320

cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 7380

agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 7440

cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 7500

cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 7560

ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 7620

taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 7680

tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 7740

ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 7800

atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 7860

gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 7920

tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 7980

cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 8040

taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 8100

ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 8160

ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 8220

cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 8280

ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 8340

gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 8400

gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 8460

aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg 8520

gagatcgatc tcccgatccc ctagggtcga ctctcagtac aatctgctct gatgccgcat 8580

agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca 8640

aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg 8700

ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat 8760

tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt 8820

tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 8880

cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 8940

gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatc 8997

<210> 6

<211> 8997

<212> DNA

<213> Artificial Sequence

<220>

<223> 4M+P199W-BE3

<400> 6

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaagcctcac 420

ttcagaaaca cagtggagcg aatgtatcga gacacattct cctacaactt ttataatgca 480

cccatccttt ctcgtcggaa taccgtctgg ctgtgctacg aagtgaaaac aaagggtccc 540

tcaaggcccc ctttggacgc aaagatcttt cgaggccagg tgtattccga acttaagtac 600

cacccagaga tgagattctt ccactggttc agcaagtgga ggaagctgca tcgtgaccag 660

gagtatgagg tcacctggta catatccttg agcccctgca caaagtgtac aagggatatg 720

gccacgttcc tggccgagga cccgaaggtt accctgacca tctttgttgc ccgcctcgcc 780

tacttccttg acccagatta ccaggaggcg cttcgcagcc tgtgtcagaa aagagacggt 840

ccgcgtgcca ccatgaagat catgaattat gacgaatttc agcactgttg gagcaagttc 900

gtgtacagcc aaagagagct atttgagcct tggaataatc tgcctaaata ttatatatta 960

ctgcacatca tgctggggga gattctcaga cactcgatgg attggcccac attcactttc 1020

aactttaaca atgaaccttg ggtcagagga cggcatgaga cttacctgtg ttatgaggtg 1080

gagcgcatgc acaatgacac ctgggtcctg ctgaaccagc gcaggggctt tctatgcaac 1140

caggctccac ataaacacgg tttccttgaa ggccgccatg cagagctgtg cttcctggac 1200

gtgattccct tttggaagct ggacctggac caggactaca gggttacctg cttcacctcc 1260

tggagcccct gcttcagctg tgcccaggaa atggctaaat tcatttcaaa aaacaaacac 1320

gtgagcctgt gcatcttcac tgcccgcatc tatgatgatc aaggaagatg tcaggagggg 1380

ctgcgcaccc tggccgaggc tggggccaaa atttcaataa tgacatacag tgaatttaag 1440

cactgctggg acacctttgt ggaccaccag ggatgtccct tccagccctg ggatggacta 1500

gatgagcaca gccaagacct gagtgggagg ctgcgggcca ttctccagaa tcaggaaaac 1560

agcggcagcg agactcccgg gacctcagag tccgccacac ccgaaagtga taaaaagtat 1620

tctattggtt tagccatcgg cactaattcc gttggatggg ctgtcataac cgatgaatac 1680

aaagtacctt caaagaaatt taaggtgttg gggaacacag accgtcattc gattaaaaag 1740

aatcttatcg gtgccctcct attcgatagt ggcgaaacgg cagaggcgac tcgcctgaaa 1800

cgaaccgctc ggagaaggta tacacgtcgc aagaaccgaa tatgttactt acaagaaatt 1860

tttagcaatg agatggccaa agttgacgat tctttctttc accgtttgga agagtccttc 1920

cttgtcgaag aggacaagaa acatgaacgg caccccatct ttggaaacat agtagatgag 1980

gtggcatatc atgaaaagta cccaacgatt tatcacctca gaaaaaagct agttgactca 2040

actgataaag cggacctgag gttaatctac ttggctcttg cccatatgat aaagttccgt 2100

gggcactttc tcattgaggg tgatctaaat ccggacaact cggatgtcga caaactgttc 2160

atccagttag tacaaaccta taatcagttg tttgaagaga accctataaa tgcaagtggc 2220

gtggatgcga aggctattct tagcgcccgc ctctctaaat cccgacggct agaaaacctg 2280

atcgcacaat tacccggaga gaagaaaaat gggttgttcg gtaaccttat agcgctctca 2340

ctaggcctga caccaaattt taagtcgaac ttcgacttag ctgaagatgc caaattgcag 2400

cttagtaagg acacgtacga tgacgatctc gacaatctac tggcacaaat tggagatcag 2460

tatgcggact tatttttggc tgccaaaaac cttagcgatg caatcctcct atctgacata 2520

ctgagagtta atactgagat taccaaggcg ccgttatccg cttcaatgat caaaaggtac 2580

gatgaacatc accaagactt gacacttctc aaggccctag tccgtcagca actgcctgag 2640

aaatataagg aaatattctt tgatcagtcg aaaaacgggt acgcaggtta tattgacggc 2700

ggagcgagtc aagaggaatt ctacaagttt atcaaaccca tattagagaa gatggatggg 2760

acggaagagt tgcttgtaaa actcaatcgc gaagatctac tgcgaaagca gcggactttc 2820

gacaacggta gcattccaca tcaaatccac ttaggcgaat tgcatgctat acttagaagg 2880

caggaggatt tttatccgtt cctcaaagac aatcgtgaaa agattgagaa aatcctaacc 2940

tttcgcatac cttactatgt gggacccctg gcccgaggga actctcggtt cgcatggatg 3000

acaagaaagt ccgaagaaac gattactcca tggaattttg aggaagttgt cgataaaggt 3060

gcgtcagctc aatcgttcat cgagaggatg accaactttg acaagaattt accgaacgaa 3120

aaagtattgc ctaagcacag tttactttac gagtatttca cagtgtacaa tgaactcacg 3180

aaagttaagt atgtcactga gggcatgcgt aaacccgcct ttctaagcgg agaacagaag 3240

aaagcaatag tagatctgtt attcaagacc aaccgcaaag tgacagttaa gcaattgaaa 3300

gaggactact ttaagaaaat tgaatgcttc gattctgtcg agatctccgg ggtagaagat 3360

cgatttaatg cgtcacttgg tacgtatcat gacctcctaa agataattaa agataaggac 3420

ttcctggata acgaagagaa tgaagatatc ttagaagata tagtgttgac tcttaccctc 3480

tttgaagatc gggaaatgat tgaggaaaga ctaaaaacat acgctcacct gttcgacgat 3540

aaggttatga aacagttaaa gaggcgtcgc tatacgggct ggggacgatt gtcgcggaaa 3600

cttatcaacg ggataagaga caagcaaagt ggtaaaacta ttctcgattt tctaaagagc 3660

gacggcttcg ccaataggaa ctttatgcag ctgatccatg atgactcttt aaccttcaaa 3720

gaggatatac aaaaggcaca ggtttccgga caaggggact cattgcacga acatattgcg 3780

aatcttgctg gttcgccagc catcaaaaag ggcatactcc agacagtcaa agtagtggat 3840

gagctagtta aggtcatggg acgtcacaaa ccggaaaaca ttgtaatcga gatggcacgc 3900

gaaaatcaaa cgactcagaa ggggcaaaaa aacagtcgag agcggatgaa gagaatagaa 3960

gagggtatta aagaactggg cagccagatc ttaaaggagc atcctgtgga aaatacccaa 4020

ttgcagaacg agaaacttta cctctattac ctacaaaatg gaagggacat gtatgttgat 4080

caggaactgg acataaaccg tttatctgat tacgacgtcg atcacattgt accccaatcc 4140

tttttgaagg acgattcaat cgacaataaa gtgcttacac gctcggataa gaaccgaggg 4200

aaaagtgaca atgttccaag cgaggaagtc gtaaagaaaa tgaagaacta ttggcggcag 4260

ctcctaaatg cgaaactgat aacgcaaaga aagttcgata acttaactaa agctgagagg 4320

ggtggcttgt ctgaacttga caaggccgga tttattaaac gtcagctcgt ggaaacccgc 4380

caaatcacaa agcatgttgc acagatacta gattcccgaa tgaatacgaa atacgacgag 4440

aacgataagc tgattcggga agtcaaagta atcactttaa agtcaaaatt ggtgtcggac 4500

ttcagaaagg attttcaatt ctataaagtt agggagataa ataactacca ccatgcgcac 4560

gacgcttatc ttaatgccgt cgtagggacc gcactcatta agaaataccc gaagctagaa 4620

agtgagtttg tgtatggtga ttacaaagtt tatgacgtcc gtaagatgat cgcgaaaagc 4680

gaacaggaga taggcaaggc tacagccaaa tacttctttt attctaacat tatgaatttc 4740

tttaagacgg aaatcactct ggcaaacgga gagatacgca aacgaccttt aattgaaacc 4800

aatggggaga caggtgaaat cgtatgggat aagggccggg acttcgcgac ggtgagaaaa 4860

gttttgtcca tgccccaagt caacatagta aagaaaactg aggtgcagac cggagggttt 4920

tcaaaggaat cgattcttcc aaaaaggaat agtgataagc tcatcgctcg taaaaaggac 4980

tgggacccga aaaagtacgg tggcttcgat agccctacag ttgcctattc tgtcctagta 5040

gtggcaaaag ttgagaaggg aaaatccaag aaactgaagt cagtcaaaga attattgggg 5100

ataacgatta tggagcgctc gtcttttgaa aagaacccca tcgacttcct tgaggcgaaa 5160

ggttacaagg aagtaaaaaa ggatctcata attaaactac caaagtatag tctgtttgag 5220

ttagaaaatg gccgaaaacg gatgttggct agcgccggag agcttcaaaa ggggaacgaa 5280

ctcgcactac cgtctaaata cgtgaatttc ctgtatttag cgtcccatta cgagaagttg 5340

aaaggttcac ctgaagataa cgaacagaag caactttttg ttgagcagca caaacattat 5400

ctcgacgaaa tcatagagca aatttcggaa ttcagtaaga gagtcatcct agctgatgcc 5460

aatctggaca aagtattaag cgcatacaac aagcacaggg ataaacccat acgtgagcag 5520

gcggaaaata ttatccattt gtttactctt accaacctcg gcgctccagc cgcattcaag 5580

tattttgaca caacgataga tcgcaaacga tacacttcta ccaaggaggt gctagacgcg 5640

acactgattc accaatccat cacgggatta tatgaaactc ggatagattt gtcacagctt 5700

gggggtgact ctggtggttc tactaatctg tcagatatta ttgaaaagga gaccggtaag 5760

caactggtta tccaggaatc catcctcatg ctcccagagg aggtggaaga agtcattggg 5820

aacaagccgg aaagcgatat actcgtgcac accgcctacg acgagagcac cgacgagaat 5880

gtcatgcttc tgactagcga cgcccctgaa tacaagcctt gggctctggt catacaggat 5940

agcaacggtg agaacaagat taagatgctc tctggtggtt ctcccaagaa gaagaggaaa 6000

gtctaaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg 6060

tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg 6120

aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga 6180

gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg 6240

aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa 6300

ccagctgggg ctcgataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc 6360

tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 6420

taaagtgtaa agcctagggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 6480

cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 6540

gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 6600

tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 6660

tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 6720

ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 6780

agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 6840

accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 6900

ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 6960

gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 7020

ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 7080

gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 7140

taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 7200

tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 7260

gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 7320

cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 7380

agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 7440

cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 7500

cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 7560

ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 7620

taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 7680

tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 7740

ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 7800

atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 7860

gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 7920

tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 7980

cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 8040

taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 8100

ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 8160

ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 8220

cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 8280

ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 8340

gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 8400

gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 8460

aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg 8520

gagatcgatc tcccgatccc ctagggtcga ctctcagtac aatctgctct gatgccgcat 8580

agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca 8640

aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg 8700

ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat 8760

tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt 8820

tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 8880

cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 8940

gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatc 8997

<210> 7

<211> 8997

<212> DNA

<213> Artificial Sequence

<220>

<223> 4M+P200A-BE3

<400> 7

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaagcctcac 420

ttcagaaaca cagtggagcg aatgtatcga gacacattct cctacaactt ttataatgca 480

cccatccttt ctcgtcggaa taccgtctgg ctgtgctacg aagtgaaaac aaagggtccc 540

tcaaggcccc ctttggacgc aaagatcttt cgaggccagg tgtattccga acttaagtac 600

cacccagaga tgagattctt ccactggttc agcaagtgga ggaagctgca tcgtgaccag 660

gagtatgagg tcacctggta catatccttg agcccctgca caaagtgtac aagggatatg 720

gccacgttcc tggccgagga cccgaaggtt accctgacca tctttgttgc ccgcctcgcc 780

tacttccttg acccagatta ccaggaggcg cttcgcagcc tgtgtcagaa aagagacggt 840

ccgcgtgcca ccatgaagat catgaattat gacgaatttc agcactgttg gagcaagttc 900

gtgtacagcc aaagagagct atttgagcct tggaataatc tgcctaaata ttatatatta 960

ctgcacatca tgctggggga gattctcaga cactcgatgg atccagccac attcactttc 1020

aactttaaca atgaaccttg ggtcagagga cggcatgaga cttacctgtg ttatgaggtg 1080

gagcgcatgc acaatgacac ctgggtcctg ctgaaccagc gcaggggctt tctatgcaac 1140

caggctccac ataaacacgg tttccttgaa ggccgccatg cagagctgtg cttcctggac 1200

gtgattccct tttggaagct ggacctggac caggactaca gggttacctg cttcacctcc 1260

tggagcccct gcttcagctg tgcccaggaa atggctaaat tcatttcaaa aaacaaacac 1320

gtgagcctgt gcatcttcac tgcccgcatc tatgatgatc aaggaagatg tcaggagggg 1380

ctgcgcaccc tggccgaggc tggggccaaa atttcaataa tgacatacag tgaatttaag 1440

cactgctggg acacctttgt ggaccaccag ggatgtccct tccagccctg ggatggacta 1500

gatgagcaca gccaagacct gagtgggagg ctgcgggcca ttctccagaa tcaggaaaac 1560

agcggcagcg agactcccgg gacctcagag tccgccacac ccgaaagtga taaaaagtat 1620

tctattggtt tagccatcgg cactaattcc gttggatggg ctgtcataac cgatgaatac 1680

aaagtacctt caaagaaatt taaggtgttg gggaacacag accgtcattc gattaaaaag 1740

aatcttatcg gtgccctcct attcgatagt ggcgaaacgg cagaggcgac tcgcctgaaa 1800

cgaaccgctc ggagaaggta tacacgtcgc aagaaccgaa tatgttactt acaagaaatt 1860

tttagcaatg agatggccaa agttgacgat tctttctttc accgtttgga agagtccttc 1920

cttgtcgaag aggacaagaa acatgaacgg caccccatct ttggaaacat agtagatgag 1980

gtggcatatc atgaaaagta cccaacgatt tatcacctca gaaaaaagct agttgactca 2040

actgataaag cggacctgag gttaatctac ttggctcttg cccatatgat aaagttccgt 2100

gggcactttc tcattgaggg tgatctaaat ccggacaact cggatgtcga caaactgttc 2160

atccagttag tacaaaccta taatcagttg tttgaagaga accctataaa tgcaagtggc 2220

gtggatgcga aggctattct tagcgcccgc ctctctaaat cccgacggct agaaaacctg 2280

atcgcacaat tacccggaga gaagaaaaat gggttgttcg gtaaccttat agcgctctca 2340

ctaggcctga caccaaattt taagtcgaac ttcgacttag ctgaagatgc caaattgcag 2400

cttagtaagg acacgtacga tgacgatctc gacaatctac tggcacaaat tggagatcag 2460

tatgcggact tatttttggc tgccaaaaac cttagcgatg caatcctcct atctgacata 2520

ctgagagtta atactgagat taccaaggcg ccgttatccg cttcaatgat caaaaggtac 2580

gatgaacatc accaagactt gacacttctc aaggccctag tccgtcagca actgcctgag 2640

aaatataagg aaatattctt tgatcagtcg aaaaacgggt acgcaggtta tattgacggc 2700

ggagcgagtc aagaggaatt ctacaagttt atcaaaccca tattagagaa gatggatggg 2760

acggaagagt tgcttgtaaa actcaatcgc gaagatctac tgcgaaagca gcggactttc 2820

gacaacggta gcattccaca tcaaatccac ttaggcgaat tgcatgctat acttagaagg 2880

caggaggatt tttatccgtt cctcaaagac aatcgtgaaa agattgagaa aatcctaacc 2940

tttcgcatac cttactatgt gggacccctg gcccgaggga actctcggtt cgcatggatg 3000

acaagaaagt ccgaagaaac gattactcca tggaattttg aggaagttgt cgataaaggt 3060

gcgtcagctc aatcgttcat cgagaggatg accaactttg acaagaattt accgaacgaa 3120

aaagtattgc ctaagcacag tttactttac gagtatttca cagtgtacaa tgaactcacg 3180

aaagttaagt atgtcactga gggcatgcgt aaacccgcct ttctaagcgg agaacagaag 3240

aaagcaatag tagatctgtt attcaagacc aaccgcaaag tgacagttaa gcaattgaaa 3300

gaggactact ttaagaaaat tgaatgcttc gattctgtcg agatctccgg ggtagaagat 3360

cgatttaatg cgtcacttgg tacgtatcat gacctcctaa agataattaa agataaggac 3420

ttcctggata acgaagagaa tgaagatatc ttagaagata tagtgttgac tcttaccctc 3480

tttgaagatc gggaaatgat tgaggaaaga ctaaaaacat acgctcacct gttcgacgat 3540

aaggttatga aacagttaaa gaggcgtcgc tatacgggct ggggacgatt gtcgcggaaa 3600

cttatcaacg ggataagaga caagcaaagt ggtaaaacta ttctcgattt tctaaagagc 3660

gacggcttcg ccaataggaa ctttatgcag ctgatccatg atgactcttt aaccttcaaa 3720

gaggatatac aaaaggcaca ggtttccgga caaggggact cattgcacga acatattgcg 3780

aatcttgctg gttcgccagc catcaaaaag ggcatactcc agacagtcaa agtagtggat 3840

gagctagtta aggtcatggg acgtcacaaa ccggaaaaca ttgtaatcga gatggcacgc 3900

gaaaatcaaa cgactcagaa ggggcaaaaa aacagtcgag agcggatgaa gagaatagaa 3960

gagggtatta aagaactggg cagccagatc ttaaaggagc atcctgtgga aaatacccaa 4020

ttgcagaacg agaaacttta cctctattac ctacaaaatg gaagggacat gtatgttgat 4080

caggaactgg acataaaccg tttatctgat tacgacgtcg atcacattgt accccaatcc 4140

tttttgaagg acgattcaat cgacaataaa gtgcttacac gctcggataa gaaccgaggg 4200

aaaagtgaca atgttccaag cgaggaagtc gtaaagaaaa tgaagaacta ttggcggcag 4260

ctcctaaatg cgaaactgat aacgcaaaga aagttcgata acttaactaa agctgagagg 4320

ggtggcttgt ctgaacttga caaggccgga tttattaaac gtcagctcgt ggaaacccgc 4380

caaatcacaa agcatgttgc acagatacta gattcccgaa tgaatacgaa atacgacgag 4440

aacgataagc tgattcggga agtcaaagta atcactttaa agtcaaaatt ggtgtcggac 4500

ttcagaaagg attttcaatt ctataaagtt agggagataa ataactacca ccatgcgcac 4560

gacgcttatc ttaatgccgt cgtagggacc gcactcatta agaaataccc gaagctagaa 4620

agtgagtttg tgtatggtga ttacaaagtt tatgacgtcc gtaagatgat cgcgaaaagc 4680

gaacaggaga taggcaaggc tacagccaaa tacttctttt attctaacat tatgaatttc 4740

tttaagacgg aaatcactct ggcaaacgga gagatacgca aacgaccttt aattgaaacc 4800

aatggggaga caggtgaaat cgtatgggat aagggccggg acttcgcgac ggtgagaaaa 4860

gttttgtcca tgccccaagt caacatagta aagaaaactg aggtgcagac cggagggttt 4920

tcaaaggaat cgattcttcc aaaaaggaat agtgataagc tcatcgctcg taaaaaggac 4980

tgggacccga aaaagtacgg tggcttcgat agccctacag ttgcctattc tgtcctagta 5040

gtggcaaaag ttgagaaggg aaaatccaag aaactgaagt cagtcaaaga attattgggg 5100

ataacgatta tggagcgctc gtcttttgaa aagaacccca tcgacttcct tgaggcgaaa 5160

ggttacaagg aagtaaaaaa ggatctcata attaaactac caaagtatag tctgtttgag 5220

ttagaaaatg gccgaaaacg gatgttggct agcgccggag agcttcaaaa ggggaacgaa 5280

ctcgcactac cgtctaaata cgtgaatttc ctgtatttag cgtcccatta cgagaagttg 5340

aaaggttcac ctgaagataa cgaacagaag caactttttg ttgagcagca caaacattat 5400

ctcgacgaaa tcatagagca aatttcggaa ttcagtaaga gagtcatcct agctgatgcc 5460

aatctggaca aagtattaag cgcatacaac aagcacaggg ataaacccat acgtgagcag 5520

gcggaaaata ttatccattt gtttactctt accaacctcg gcgctccagc cgcattcaag 5580

tattttgaca caacgataga tcgcaaacga tacacttcta ccaaggaggt gctagacgcg 5640

acactgattc accaatccat cacgggatta tatgaaactc ggatagattt gtcacagctt 5700

gggggtgact ctggtggttc tactaatctg tcagatatta ttgaaaagga gaccggtaag 5760

caactggtta tccaggaatc catcctcatg ctcccagagg aggtggaaga agtcattggg 5820

aacaagccgg aaagcgatat actcgtgcac accgcctacg acgagagcac cgacgagaat 5880

gtcatgcttc tgactagcga cgcccctgaa tacaagcctt gggctctggt catacaggat 5940

agcaacggtg agaacaagat taagatgctc tctggtggtt ctcccaagaa gaagaggaaa 6000

gtctaaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg 6060

tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg 6120

aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga 6180

gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg 6240

aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa 6300

ccagctgggg ctcgataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc 6360

tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 6420

taaagtgtaa agcctagggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 6480

cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 6540

gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 6600

tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 6660

tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 6720

ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 6780

agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 6840

accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 6900

ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 6960

gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 7020

ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 7080

gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 7140

taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 7200

tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 7260

gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 7320

cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 7380

agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 7440

cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 7500

cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 7560

ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 7620

taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 7680

tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 7740

ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 7800

atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 7860

gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 7920

tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 7980

cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 8040

taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 8100

ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 8160

ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 8220

cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 8280

ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 8340

gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 8400

gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 8460

aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg 8520

gagatcgatc tcccgatccc ctagggtcga ctctcagtac aatctgctct gatgccgcat 8580

agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca 8640

aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg 8700

ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat 8760

tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt 8820

tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 8880

cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 8940

gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatc 8997

<210> 8

<211> 8997

<212> DNA

<213> Artificial Sequence

<220>

<223> 4M+P200K-BE3

<400> 8

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaagcctcac 420

ttcagaaaca cagtggagcg aatgtatcga gacacattct cctacaactt ttataatgca 480

cccatccttt ctcgtcggaa taccgtctgg ctgtgctacg aagtgaaaac aaagggtccc 540

tcaaggcccc ctttggacgc aaagatcttt cgaggccagg tgtattccga acttaagtac 600

cacccagaga tgagattctt ccactggttc agcaagtgga ggaagctgca tcgtgaccag 660

gagtatgagg tcacctggta catatccttg agcccctgca caaagtgtac aagggatatg 720

gccacgttcc tggccgagga cccgaaggtt accctgacca tctttgttgc ccgcctcgcc 780

tacttccttg acccagatta ccaggaggcg cttcgcagcc tgtgtcagaa aagagacggt 840

ccgcgtgcca ccatgaagat catgaattat gacgaatttc agcactgttg gagcaagttc 900

gtgtacagcc aaagagagct atttgagcct tggaataatc tgcctaaata ttatatatta 960

ctgcacatca tgctggggga gattctcaga cactcgatgg atccaaagac attcactttc 1020

aactttaaca atgaaccttg ggtcagagga cggcatgaga cttacctgtg ttatgaggtg 1080

gagcgcatgc acaatgacac ctgggtcctg ctgaaccagc gcaggggctt tctatgcaac 1140

caggctccac ataaacacgg tttccttgaa ggccgccatg cagagctgtg cttcctggac 1200

gtgattccct tttggaagct ggacctggac caggactaca gggttacctg cttcacctcc 1260

tggagcccct gcttcagctg tgcccaggaa atggctaaat tcatttcaaa aaacaaacac 1320

gtgagcctgt gcatcttcac tgcccgcatc tatgatgatc aaggaagatg tcaggagggg 1380

ctgcgcaccc tggccgaggc tggggccaaa atttcaataa tgacatacag tgaatttaag 1440

cactgctggg acacctttgt ggaccaccag ggatgtccct tccagccctg ggatggacta 1500

gatgagcaca gccaagacct gagtgggagg ctgcgggcca ttctccagaa tcaggaaaac 1560

agcggcagcg agactcccgg gacctcagag tccgccacac ccgaaagtga taaaaagtat 1620

tctattggtt tagccatcgg cactaattcc gttggatggg ctgtcataac cgatgaatac 1680

aaagtacctt caaagaaatt taaggtgttg gggaacacag accgtcattc gattaaaaag 1740

aatcttatcg gtgccctcct attcgatagt ggcgaaacgg cagaggcgac tcgcctgaaa 1800

cgaaccgctc ggagaaggta tacacgtcgc aagaaccgaa tatgttactt acaagaaatt 1860

tttagcaatg agatggccaa agttgacgat tctttctttc accgtttgga agagtccttc 1920

cttgtcgaag aggacaagaa acatgaacgg caccccatct ttggaaacat agtagatgag 1980

gtggcatatc atgaaaagta cccaacgatt tatcacctca gaaaaaagct agttgactca 2040

actgataaag cggacctgag gttaatctac ttggctcttg cccatatgat aaagttccgt 2100

gggcactttc tcattgaggg tgatctaaat ccggacaact cggatgtcga caaactgttc 2160

atccagttag tacaaaccta taatcagttg tttgaagaga accctataaa tgcaagtggc 2220

gtggatgcga aggctattct tagcgcccgc ctctctaaat cccgacggct agaaaacctg 2280

atcgcacaat tacccggaga gaagaaaaat gggttgttcg gtaaccttat agcgctctca 2340

ctaggcctga caccaaattt taagtcgaac ttcgacttag ctgaagatgc caaattgcag 2400

cttagtaagg acacgtacga tgacgatctc gacaatctac tggcacaaat tggagatcag 2460

tatgcggact tatttttggc tgccaaaaac cttagcgatg caatcctcct atctgacata 2520

ctgagagtta atactgagat taccaaggcg ccgttatccg cttcaatgat caaaaggtac 2580

gatgaacatc accaagactt gacacttctc aaggccctag tccgtcagca actgcctgag 2640

aaatataagg aaatattctt tgatcagtcg aaaaacgggt acgcaggtta tattgacggc 2700

ggagcgagtc aagaggaatt ctacaagttt atcaaaccca tattagagaa gatggatggg 2760

acggaagagt tgcttgtaaa actcaatcgc gaagatctac tgcgaaagca gcggactttc 2820

gacaacggta gcattccaca tcaaatccac ttaggcgaat tgcatgctat acttagaagg 2880

caggaggatt tttatccgtt cctcaaagac aatcgtgaaa agattgagaa aatcctaacc 2940

tttcgcatac cttactatgt gggacccctg gcccgaggga actctcggtt cgcatggatg 3000

acaagaaagt ccgaagaaac gattactcca tggaattttg aggaagttgt cgataaaggt 3060

gcgtcagctc aatcgttcat cgagaggatg accaactttg acaagaattt accgaacgaa 3120

aaagtattgc ctaagcacag tttactttac gagtatttca cagtgtacaa tgaactcacg 3180

aaagttaagt atgtcactga gggcatgcgt aaacccgcct ttctaagcgg agaacagaag 3240

aaagcaatag tagatctgtt attcaagacc aaccgcaaag tgacagttaa gcaattgaaa 3300

gaggactact ttaagaaaat tgaatgcttc gattctgtcg agatctccgg ggtagaagat 3360

cgatttaatg cgtcacttgg tacgtatcat gacctcctaa agataattaa agataaggac 3420

ttcctggata acgaagagaa tgaagatatc ttagaagata tagtgttgac tcttaccctc 3480

tttgaagatc gggaaatgat tgaggaaaga ctaaaaacat acgctcacct gttcgacgat 3540

aaggttatga aacagttaaa gaggcgtcgc tatacgggct ggggacgatt gtcgcggaaa 3600

cttatcaacg ggataagaga caagcaaagt ggtaaaacta ttctcgattt tctaaagagc 3660

gacggcttcg ccaataggaa ctttatgcag ctgatccatg atgactcttt aaccttcaaa 3720

gaggatatac aaaaggcaca ggtttccgga caaggggact cattgcacga acatattgcg 3780

aatcttgctg gttcgccagc catcaaaaag ggcatactcc agacagtcaa agtagtggat 3840

gagctagtta aggtcatggg acgtcacaaa ccggaaaaca ttgtaatcga gatggcacgc 3900

gaaaatcaaa cgactcagaa ggggcaaaaa aacagtcgag agcggatgaa gagaatagaa 3960

gagggtatta aagaactggg cagccagatc ttaaaggagc atcctgtgga aaatacccaa 4020

ttgcagaacg agaaacttta cctctattac ctacaaaatg gaagggacat gtatgttgat 4080

caggaactgg acataaaccg tttatctgat tacgacgtcg atcacattgt accccaatcc 4140

tttttgaagg acgattcaat cgacaataaa gtgcttacac gctcggataa gaaccgaggg 4200

aaaagtgaca atgttccaag cgaggaagtc gtaaagaaaa tgaagaacta ttggcggcag 4260

ctcctaaatg cgaaactgat aacgcaaaga aagttcgata acttaactaa agctgagagg 4320

ggtggcttgt ctgaacttga caaggccgga tttattaaac gtcagctcgt ggaaacccgc 4380

caaatcacaa agcatgttgc acagatacta gattcccgaa tgaatacgaa atacgacgag 4440

aacgataagc tgattcggga agtcaaagta atcactttaa agtcaaaatt ggtgtcggac 4500

ttcagaaagg attttcaatt ctataaagtt agggagataa ataactacca ccatgcgcac 4560

gacgcttatc ttaatgccgt cgtagggacc gcactcatta agaaataccc gaagctagaa 4620

agtgagtttg tgtatggtga ttacaaagtt tatgacgtcc gtaagatgat cgcgaaaagc 4680

gaacaggaga taggcaaggc tacagccaaa tacttctttt attctaacat tatgaatttc 4740

tttaagacgg aaatcactct ggcaaacgga gagatacgca aacgaccttt aattgaaacc 4800

aatggggaga caggtgaaat cgtatgggat aagggccggg acttcgcgac ggtgagaaaa 4860

gttttgtcca tgccccaagt caacatagta aagaaaactg aggtgcagac cggagggttt 4920

tcaaaggaat cgattcttcc aaaaaggaat agtgataagc tcatcgctcg taaaaaggac 4980

tgggacccga aaaagtacgg tggcttcgat agccctacag ttgcctattc tgtcctagta 5040

gtggcaaaag ttgagaaggg aaaatccaag aaactgaagt cagtcaaaga attattgggg 5100

ataacgatta tggagcgctc gtcttttgaa aagaacccca tcgacttcct tgaggcgaaa 5160

ggttacaagg aagtaaaaaa ggatctcata attaaactac caaagtatag tctgtttgag 5220

ttagaaaatg gccgaaaacg gatgttggct agcgccggag agcttcaaaa ggggaacgaa 5280

ctcgcactac cgtctaaata cgtgaatttc ctgtatttag cgtcccatta cgagaagttg 5340

aaaggttcac ctgaagataa cgaacagaag caactttttg ttgagcagca caaacattat 5400

ctcgacgaaa tcatagagca aatttcggaa ttcagtaaga gagtcatcct agctgatgcc 5460

aatctggaca aagtattaag cgcatacaac aagcacaggg ataaacccat acgtgagcag 5520

gcggaaaata ttatccattt gtttactctt accaacctcg gcgctccagc cgcattcaag 5580

tattttgaca caacgataga tcgcaaacga tacacttcta ccaaggaggt gctagacgcg 5640

acactgattc accaatccat cacgggatta tatgaaactc ggatagattt gtcacagctt 5700

gggggtgact ctggtggttc tactaatctg tcagatatta ttgaaaagga gaccggtaag 5760

caactggtta tccaggaatc catcctcatg ctcccagagg aggtggaaga agtcattggg 5820

aacaagccgg aaagcgatat actcgtgcac accgcctacg acgagagcac cgacgagaat 5880

gtcatgcttc tgactagcga cgcccctgaa tacaagcctt gggctctggt catacaggat 5940

agcaacggtg agaacaagat taagatgctc tctggtggtt ctcccaagaa gaagaggaaa 6000

gtctaaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg 6060

tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg 6120

aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga 6180

gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg 6240

aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa 6300

ccagctgggg ctcgataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc 6360

tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 6420

taaagtgtaa agcctagggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 6480

cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 6540

gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 6600

tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 6660

tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 6720

ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 6780

agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 6840

accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 6900

ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 6960

gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 7020

ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 7080

gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 7140

taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 7200

tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 7260

gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 7320

cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 7380

agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 7440

cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 7500

cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 7560

ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 7620

taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 7680

tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 7740

ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 7800

atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 7860

gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 7920

tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 7980

cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 8040

taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 8100

ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 8160

ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 8220

cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 8280

ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 8340

gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 8400

gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 8460

aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg 8520

gagatcgatc tcccgatccc ctagggtcga ctctcagtac aatctgctct gatgccgcat 8580

agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca 8640

aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg 8700

ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat 8760

tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt 8820

tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 8880

cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 8940

gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatc 8997

<210> 9

<211> 8997

<212> DNA

<213> Artificial Sequence

<220>

<223> 4M+Q322K-BE3

<400> 9

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaagcctcac 420

ttcagaaaca cagtggagcg aatgtatcga gacacattct cctacaactt ttataatgca 480

cccatccttt ctcgtcggaa taccgtctgg ctgtgctacg aagtgaaaac aaagggtccc 540

tcaaggcccc ctttggacgc aaagatcttt cgaggccagg tgtattccga acttaagtac 600

cacccagaga tgagattctt ccactggttc agcaagtgga ggaagctgca tcgtgaccag 660

gagtatgagg tcacctggta catatccttg agcccctgca caaagtgtac aagggatatg 720

gccacgttcc tggccgagga cccgaaggtt accctgacca tctttgttgc ccgcctcgcc 780

tacttccttg acccagatta ccaggaggcg cttcgcagcc tgtgtcagaa aagagacggt 840

ccgcgtgcca ccatgaagat catgaattat gacgaatttc agcactgttg gagcaagttc 900

gtgtacagcc aaagagagct atttgagcct tggaataatc tgcctaaata ttatatatta 960

ctgcacatca tgctggggga gattctcaga cactcgatgg atccacccac attcactttc 1020

aactttaaca atgaaccttg ggtcagagga cggcatgaga cttacctgtg ttatgaggtg 1080

gagcgcatgc acaatgacac ctgggtcctg ctgaaccagc gcaggggctt tctatgcaac 1140

caggctccac ataaacacgg tttccttgaa ggccgccatg cagagctgtg cttcctggac 1200

gtgattccct tttggaagct ggacctggac caggactaca gggttacctg cttcacctcc 1260

tggagcccct gcttcagctg tgcccaggaa atggctaaat tcatttcaaa aaacaaacac 1320

gtgagcctgt gcatcttcac tgcccgcatc tatgatgatc aaggaagatg taaggagggg 1380

ctgcgcaccc tggccgaggc tggggccaaa atttcaataa tgacatacag tgaatttaag 1440

cactgctggg acacctttgt ggaccaccag ggatgtccct tccagccctg ggatggacta 1500

gatgagcaca gccaagacct gagtgggagg ctgcgggcca ttctccagaa tcaggaaaac 1560

agcggcagcg agactcccgg gacctcagag tccgccacac ccgaaagtga taaaaagtat 1620

tctattggtt tagccatcgg cactaattcc gttggatggg ctgtcataac cgatgaatac 1680

aaagtacctt caaagaaatt taaggtgttg gggaacacag accgtcattc gattaaaaag 1740

aatcttatcg gtgccctcct attcgatagt ggcgaaacgg cagaggcgac tcgcctgaaa 1800

cgaaccgctc ggagaaggta tacacgtcgc aagaaccgaa tatgttactt acaagaaatt 1860

tttagcaatg agatggccaa agttgacgat tctttctttc accgtttgga agagtccttc 1920

cttgtcgaag aggacaagaa acatgaacgg caccccatct ttggaaacat agtagatgag 1980

gtggcatatc atgaaaagta cccaacgatt tatcacctca gaaaaaagct agttgactca 2040

actgataaag cggacctgag gttaatctac ttggctcttg cccatatgat aaagttccgt 2100

gggcactttc tcattgaggg tgatctaaat ccggacaact cggatgtcga caaactgttc 2160

atccagttag tacaaaccta taatcagttg tttgaagaga accctataaa tgcaagtggc 2220

gtggatgcga aggctattct tagcgcccgc ctctctaaat cccgacggct agaaaacctg 2280

atcgcacaat tacccggaga gaagaaaaat gggttgttcg gtaaccttat agcgctctca 2340

ctaggcctga caccaaattt taagtcgaac ttcgacttag ctgaagatgc caaattgcag 2400

cttagtaagg acacgtacga tgacgatctc gacaatctac tggcacaaat tggagatcag 2460

tatgcggact tatttttggc tgccaaaaac cttagcgatg caatcctcct atctgacata 2520

ctgagagtta atactgagat taccaaggcg ccgttatccg cttcaatgat caaaaggtac 2580

gatgaacatc accaagactt gacacttctc aaggccctag tccgtcagca actgcctgag 2640

aaatataagg aaatattctt tgatcagtcg aaaaacgggt acgcaggtta tattgacggc 2700

ggagcgagtc aagaggaatt ctacaagttt atcaaaccca tattagagaa gatggatggg 2760

acggaagagt tgcttgtaaa actcaatcgc gaagatctac tgcgaaagca gcggactttc 2820

gacaacggta gcattccaca tcaaatccac ttaggcgaat tgcatgctat acttagaagg 2880

caggaggatt tttatccgtt cctcaaagac aatcgtgaaa agattgagaa aatcctaacc 2940

tttcgcatac cttactatgt gggacccctg gcccgaggga actctcggtt cgcatggatg 3000

acaagaaagt ccgaagaaac gattactcca tggaattttg aggaagttgt cgataaaggt 3060

gcgtcagctc aatcgttcat cgagaggatg accaactttg acaagaattt accgaacgaa 3120

aaagtattgc ctaagcacag tttactttac gagtatttca cagtgtacaa tgaactcacg 3180

aaagttaagt atgtcactga gggcatgcgt aaacccgcct ttctaagcgg agaacagaag 3240

aaagcaatag tagatctgtt attcaagacc aaccgcaaag tgacagttaa gcaattgaaa 3300

gaggactact ttaagaaaat tgaatgcttc gattctgtcg agatctccgg ggtagaagat 3360

cgatttaatg cgtcacttgg tacgtatcat gacctcctaa agataattaa agataaggac 3420

ttcctggata acgaagagaa tgaagatatc ttagaagata tagtgttgac tcttaccctc 3480

tttgaagatc gggaaatgat tgaggaaaga ctaaaaacat acgctcacct gttcgacgat 3540

aaggttatga aacagttaaa gaggcgtcgc tatacgggct ggggacgatt gtcgcggaaa 3600

cttatcaacg ggataagaga caagcaaagt ggtaaaacta ttctcgattt tctaaagagc 3660

gacggcttcg ccaataggaa ctttatgcag ctgatccatg atgactcttt aaccttcaaa 3720

gaggatatac aaaaggcaca ggtttccgga caaggggact cattgcacga acatattgcg 3780

aatcttgctg gttcgccagc catcaaaaag ggcatactcc agacagtcaa agtagtggat 3840

gagctagtta aggtcatggg acgtcacaaa ccggaaaaca ttgtaatcga gatggcacgc 3900

gaaaatcaaa cgactcagaa ggggcaaaaa aacagtcgag agcggatgaa gagaatagaa 3960

gagggtatta aagaactggg cagccagatc ttaaaggagc atcctgtgga aaatacccaa 4020

ttgcagaacg agaaacttta cctctattac ctacaaaatg gaagggacat gtatgttgat 4080

caggaactgg acataaaccg tttatctgat tacgacgtcg atcacattgt accccaatcc 4140

tttttgaagg acgattcaat cgacaataaa gtgcttacac gctcggataa gaaccgaggg 4200

aaaagtgaca atgttccaag cgaggaagtc gtaaagaaaa tgaagaacta ttggcggcag 4260

ctcctaaatg cgaaactgat aacgcaaaga aagttcgata acttaactaa agctgagagg 4320

ggtggcttgt ctgaacttga caaggccgga tttattaaac gtcagctcgt ggaaacccgc 4380

caaatcacaa agcatgttgc acagatacta gattcccgaa tgaatacgaa atacgacgag 4440

aacgataagc tgattcggga agtcaaagta atcactttaa agtcaaaatt ggtgtcggac 4500

ttcagaaagg attttcaatt ctataaagtt agggagataa ataactacca ccatgcgcac 4560

gacgcttatc ttaatgccgt cgtagggacc gcactcatta agaaataccc gaagctagaa 4620

agtgagtttg tgtatggtga ttacaaagtt tatgacgtcc gtaagatgat cgcgaaaagc 4680

gaacaggaga taggcaaggc tacagccaaa tacttctttt attctaacat tatgaatttc 4740

tttaagacgg aaatcactct ggcaaacgga gagatacgca aacgaccttt aattgaaacc 4800

aatggggaga caggtgaaat cgtatgggat aagggccggg acttcgcgac ggtgagaaaa 4860

gttttgtcca tgccccaagt caacatagta aagaaaactg aggtgcagac cggagggttt 4920

tcaaaggaat cgattcttcc aaaaaggaat agtgataagc tcatcgctcg taaaaaggac 4980

tgggacccga aaaagtacgg tggcttcgat agccctacag ttgcctattc tgtcctagta 5040

gtggcaaaag ttgagaaggg aaaatccaag aaactgaagt cagtcaaaga attattgggg 5100

ataacgatta tggagcgctc gtcttttgaa aagaacccca tcgacttcct tgaggcgaaa 5160

ggttacaagg aagtaaaaaa ggatctcata attaaactac caaagtatag tctgtttgag 5220

ttagaaaatg gccgaaaacg gatgttggct agcgccggag agcttcaaaa ggggaacgaa 5280

ctcgcactac cgtctaaata cgtgaatttc ctgtatttag cgtcccatta cgagaagttg 5340

aaaggttcac ctgaagataa cgaacagaag caactttttg ttgagcagca caaacattat 5400

ctcgacgaaa tcatagagca aatttcggaa ttcagtaaga gagtcatcct agctgatgcc 5460

aatctggaca aagtattaag cgcatacaac aagcacaggg ataaacccat acgtgagcag 5520

gcggaaaata ttatccattt gtttactctt accaacctcg gcgctccagc cgcattcaag 5580

tattttgaca caacgataga tcgcaaacga tacacttcta ccaaggaggt gctagacgcg 5640

acactgattc accaatccat cacgggatta tatgaaactc ggatagattt gtcacagctt 5700

gggggtgact ctggtggttc tactaatctg tcagatatta ttgaaaagga gaccggtaag 5760

caactggtta tccaggaatc catcctcatg ctcccagagg aggtggaaga agtcattggg 5820

aacaagccgg aaagcgatat actcgtgcac accgcctacg acgagagcac cgacgagaat 5880

gtcatgcttc tgactagcga cgcccctgaa tacaagcctt gggctctggt catacaggat 5940

agcaacggtg agaacaagat taagatgctc tctggtggtt ctcccaagaa gaagaggaaa 6000

gtctaaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg 6060

tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg 6120

aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga 6180

gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg 6240

aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa 6300

ccagctgggg ctcgataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc 6360

tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 6420

taaagtgtaa agcctagggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 6480

cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 6540

gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 6600

tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 6660

tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 6720

ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 6780

agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 6840

accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 6900

ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 6960

gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 7020

ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 7080

gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 7140

taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 7200

tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 7260

gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 7320

cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 7380

agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 7440

cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 7500

cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 7560

ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 7620

taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 7680

tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 7740

ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 7800

atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 7860

gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 7920

tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 7980

cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 8040

taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 8100

ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 8160

ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 8220

cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 8280

ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 8340

gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 8400

gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 8460

aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg 8520

gagatcgatc tcccgatccc ctagggtcga ctctcagtac aatctgctct gatgccgcat 8580

agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca 8640

aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg 8700

ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat 8760

tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt 8820

tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 8880

cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 8940

gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatc 8997

<210> 10

<211> 8997

<212> DNA

<213> Artificial Sequence

<220>

<223> 4M+D128K+P199A+P200A-BE3

<400> 10

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaagcctcac 420

ttcagaaaca cagtggagcg aatgtatcga gacacattct cctacaactt ttataatgca 480

cccatccttt ctcgtcggaa taccgtctgg ctgtgctacg aagtgaaaac aaagggtccc 540

tcaaggcccc ctttggacgc aaagatcttt cgaggccagg tgtattccga acttaagtac 600

cacccagaga tgagattctt ccactggttc agcaagtgga ggaagctgca tcgtgaccag 660

gagtatgagg tcacctggta catatccttg agcccctgca caaagtgtac aagggatatg 720

gccacgttcc tggccgagga cccgaaggtt accctgacca tctttgttgc ccgcctcgcc 780

tacttcctta agccagatta ccaggaggcg cttcgcagcc tgtgtcagaa aagagacggt 840

ccgcgtgcca ccatgaagat catgaattat gacgaatttc agcactgttg gagcaagttc 900

gtgtacagcc aaagagagct atttgagcct tggaataatc tgcctaaata ttatatatta 960

ctgcacatca tgctggggga gattctcaga cactcgatgg atgccaagac attcactttc 1020

aactttaaca atgaaccttg ggtcagagga cggcatgaga cttacctgtg ttatgaggtg 1080

gagcgcatgc acaatgacac ctgggtcctg ctgaaccagc gcaggggctt tctatgcaac 1140

caggctccac ataaacacgg tttccttgaa ggccgccatg cagagctgtg cttcctggac 1200

gtgattccct tttggaagct ggacctggac caggactaca gggttacctg cttcacctcc 1260

tggagcccct gcttcagctg tgcccaggaa atggctaaat tcatttcaaa aaacaaacac 1320

gtgagcctgt gcatcttcac tgcccgcatc tatgatgatc aaggaagatg tcaggagggg 1380

ctgcgcaccc tggccgaggc tggggccaaa atttcaataa tgacatacag tgaatttaag 1440

cactgctggg acacctttgt ggaccaccag ggatgtccct tccagccctg ggatggacta 1500

gatgagcaca gccaagacct gagtgggagg ctgcgggcca ttctccagaa tcaggaaaac 1560

agcggcagcg agactcccgg gacctcagag tccgccacac ccgaaagtga taaaaagtat 1620

tctattggtt tagccatcgg cactaattcc gttggatggg ctgtcataac cgatgaatac 1680

aaagtacctt caaagaaatt taaggtgttg gggaacacag accgtcattc gattaaaaag 1740

aatcttatcg gtgccctcct attcgatagt ggcgaaacgg cagaggcgac tcgcctgaaa 1800

cgaaccgctc ggagaaggta tacacgtcgc aagaaccgaa tatgttactt acaagaaatt 1860

tttagcaatg agatggccaa agttgacgat tctttctttc accgtttgga agagtccttc 1920

cttgtcgaag aggacaagaa acatgaacgg caccccatct ttggaaacat agtagatgag 1980

gtggcatatc atgaaaagta cccaacgatt tatcacctca gaaaaaagct agttgactca 2040

actgataaag cggacctgag gttaatctac ttggctcttg cccatatgat aaagttccgt 2100

gggcactttc tcattgaggg tgatctaaat ccggacaact cggatgtcga caaactgttc 2160

atccagttag tacaaaccta taatcagttg tttgaagaga accctataaa tgcaagtggc 2220

gtggatgcga aggctattct tagcgcccgc ctctctaaat cccgacggct agaaaacctg 2280

atcgcacaat tacccggaga gaagaaaaat gggttgttcg gtaaccttat agcgctctca 2340

ctaggcctga caccaaattt taagtcgaac ttcgacttag ctgaagatgc caaattgcag 2400

cttagtaagg acacgtacga tgacgatctc gacaatctac tggcacaaat tggagatcag 2460

tatgcggact tatttttggc tgccaaaaac cttagcgatg caatcctcct atctgacata 2520

ctgagagtta atactgagat taccaaggcg ccgttatccg cttcaatgat caaaaggtac 2580

gatgaacatc accaagactt gacacttctc aaggccctag tccgtcagca actgcctgag 2640

aaatataagg aaatattctt tgatcagtcg aaaaacgggt acgcaggtta tattgacggc 2700

ggagcgagtc aagaggaatt ctacaagttt atcaaaccca tattagagaa gatggatggg 2760

acggaagagt tgcttgtaaa actcaatcgc gaagatctac tgcgaaagca gcggactttc 2820

gacaacggta gcattccaca tcaaatccac ttaggcgaat tgcatgctat acttagaagg 2880

caggaggatt tttatccgtt cctcaaagac aatcgtgaaa agattgagaa aatcctaacc 2940

tttcgcatac cttactatgt gggacccctg gcccgaggga actctcggtt cgcatggatg 3000

acaagaaagt ccgaagaaac gattactcca tggaattttg aggaagttgt cgataaaggt 3060

gcgtcagctc aatcgttcat cgagaggatg accaactttg acaagaattt accgaacgaa 3120

aaagtattgc ctaagcacag tttactttac gagtatttca cagtgtacaa tgaactcacg 3180

aaagttaagt atgtcactga gggcatgcgt aaacccgcct ttctaagcgg agaacagaag 3240

aaagcaatag tagatctgtt attcaagacc aaccgcaaag tgacagttaa gcaattgaaa 3300

gaggactact ttaagaaaat tgaatgcttc gattctgtcg agatctccgg ggtagaagat 3360

cgatttaatg cgtcacttgg tacgtatcat gacctcctaa agataattaa agataaggac 3420

ttcctggata acgaagagaa tgaagatatc ttagaagata tagtgttgac tcttaccctc 3480

tttgaagatc gggaaatgat tgaggaaaga ctaaaaacat acgctcacct gttcgacgat 3540

aaggttatga aacagttaaa gaggcgtcgc tatacgggct ggggacgatt gtcgcggaaa 3600

cttatcaacg ggataagaga caagcaaagt ggtaaaacta ttctcgattt tctaaagagc 3660

gacggcttcg ccaataggaa ctttatgcag ctgatccatg atgactcttt aaccttcaaa 3720

gaggatatac aaaaggcaca ggtttccgga caaggggact cattgcacga acatattgcg 3780

aatcttgctg gttcgccagc catcaaaaag ggcatactcc agacagtcaa agtagtggat 3840

gagctagtta aggtcatggg acgtcacaaa ccggaaaaca ttgtaatcga gatggcacgc 3900

gaaaatcaaa cgactcagaa ggggcaaaaa aacagtcgag agcggatgaa gagaatagaa 3960

gagggtatta aagaactggg cagccagatc ttaaaggagc atcctgtgga aaatacccaa 4020

ttgcagaacg agaaacttta cctctattac ctacaaaatg gaagggacat gtatgttgat 4080

caggaactgg acataaaccg tttatctgat tacgacgtcg atcacattgt accccaatcc 4140

tttttgaagg acgattcaat cgacaataaa gtgcttacac gctcggataa gaaccgaggg 4200

aaaagtgaca atgttccaag cgaggaagtc gtaaagaaaa tgaagaacta ttggcggcag 4260

ctcctaaatg cgaaactgat aacgcaaaga aagttcgata acttaactaa agctgagagg 4320

ggtggcttgt ctgaacttga caaggccgga tttattaaac gtcagctcgt ggaaacccgc 4380

caaatcacaa agcatgttgc acagatacta gattcccgaa tgaatacgaa atacgacgag 4440

aacgataagc tgattcggga agtcaaagta atcactttaa agtcaaaatt ggtgtcggac 4500

ttcagaaagg attttcaatt ctataaagtt agggagataa ataactacca ccatgcgcac 4560

gacgcttatc ttaatgccgt cgtagggacc gcactcatta agaaataccc gaagctagaa 4620

agtgagtttg tgtatggtga ttacaaagtt tatgacgtcc gtaagatgat cgcgaaaagc 4680

gaacaggaga taggcaaggc tacagccaaa tacttctttt attctaacat tatgaatttc 4740

tttaagacgg aaatcactct ggcaaacgga gagatacgca aacgaccttt aattgaaacc 4800

aatggggaga caggtgaaat cgtatgggat aagggccggg acttcgcgac ggtgagaaaa 4860

gttttgtcca tgccccaagt caacatagta aagaaaactg aggtgcagac cggagggttt 4920

tcaaaggaat cgattcttcc aaaaaggaat agtgataagc tcatcgctcg taaaaaggac 4980

tgggacccga aaaagtacgg tggcttcgat agccctacag ttgcctattc tgtcctagta 5040

gtggcaaaag ttgagaaggg aaaatccaag aaactgaagt cagtcaaaga attattgggg 5100

ataacgatta tggagcgctc gtcttttgaa aagaacccca tcgacttcct tgaggcgaaa 5160

ggttacaagg aagtaaaaaa ggatctcata attaaactac caaagtatag tctgtttgag 5220

ttagaaaatg gccgaaaacg gatgttggct agcgccggag agcttcaaaa ggggaacgaa 5280

ctcgcactac cgtctaaata cgtgaatttc ctgtatttag cgtcccatta cgagaagttg 5340

aaaggttcac ctgaagataa cgaacagaag caactttttg ttgagcagca caaacattat 5400

ctcgacgaaa tcatagagca aatttcggaa ttcagtaaga gagtcatcct agctgatgcc 5460

aatctggaca aagtattaag cgcatacaac aagcacaggg ataaacccat acgtgagcag 5520

gcggaaaata ttatccattt gtttactctt accaacctcg gcgctccagc cgcattcaag 5580

tattttgaca caacgataga tcgcaaacga tacacttcta ccaaggaggt gctagacgcg 5640

acactgattc accaatccat cacgggatta tatgaaactc ggatagattt gtcacagctt 5700

gggggtgact ctggtggttc tactaatctg tcagatatta ttgaaaagga gaccggtaag 5760

caactggtta tccaggaatc catcctcatg ctcccagagg aggtggaaga agtcattggg 5820

aacaagccgg aaagcgatat actcgtgcac accgcctacg acgagagcac cgacgagaat 5880

gtcatgcttc tgactagcga cgcccctgaa tacaagcctt gggctctggt catacaggat 5940

agcaacggtg agaacaagat taagatgctc tctggtggtt ctcccaagaa gaagaggaaa 6000

gtctaaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg 6060

tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg 6120

aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga 6180

gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg 6240

aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa 6300

ccagctgggg ctcgataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc 6360

tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 6420

taaagtgtaa agcctagggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 6480

cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 6540

gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 6600

tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 6660

tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 6720

ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 6780

agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 6840

accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 6900

ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 6960

gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 7020

ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 7080

gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 7140

taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 7200

tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 7260

gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 7320

cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 7380

agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 7440

cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 7500

cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 7560

ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 7620

taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 7680

tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 7740

ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 7800

atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 7860

gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 7920

tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 7980

cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 8040

taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 8100

ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 8160

ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 8220

cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 8280

ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 8340

gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 8400

gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 8460

aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg 8520

gagatcgatc tcccgatccc ctagggtcga ctctcagtac aatctgctct gatgccgcat 8580

agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca 8640

aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg 8700

ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat 8760

tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt 8820

tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 8880

cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 8940

gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatc 8997

<210> 11

<211> 9429

<212> DNA

<213> Artificial Sequence

<220>

<223> A3G-BE4max

<400> 11

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca 420

gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcatgaa gcctcacttc 480

agaaacacag tggagcgaat gtatcgagac acattctcct acaactttta taatgcaccc 540

atcctttctc gtcggaatac cgtctggctg tgctacgaag tgaaaacaaa gggtccctca 600

aggccccctt tggacgcaaa gatctttcga ggccaggtgt attccgaact taagtaccac 660

ccagagatga gattcttcca ctggttcagc aagtggagga agctgcatcg tgaccaggag 720

tatgaggtca cctggtacat atcctggagc ccctgcacaa agtgtacaag ggatatggcc 780

acgttcctgg ccgaggaccc gaaggttacc ctgaccatct ttgttgcccg cctctactac 840

ttctgggacc cagattacca ggaggcgctt cgcagcctgt gtcagaaaag agacggtccg 900

cgtgccacca tgaagatcat gaattatgac gaatttcagc actgttggag caagttcgtg 960

tacagccaaa gagagctatt tgagccttgg aataatctgc ctaaatatta tatattactg 1020

cacatcatgc tgggggagat tctcagacac tcgatggatc cacccacatt cactttcaac 1080

tttaacaatg aaccttgggt cagaggacgg catgagactt acctgtgtta tgaggtggag 1140

cgcatgcaca atgacacctg ggtcctgctg aaccagcgca ggggctttct atgcaaccag 1200

gctccacata aacacggttt ccttgaaggc cgccatgcag agctgtgctt cctggacgtg 1260

attccctttt ggaagctgga cctggaccag gactacaggg ttacctgctt cacctcctgg 1320

agcccctgct tcagctgtgc ccaggaaatg gctaaattca tttcaaaaaa caaacacgtg 1380

agcctgtgca tcttcactgc ccgcatctat gatgatcaag gaagatgtca ggaggggctg 1440

cgcaccctgg ccgaggctgg ggccaaaatt tcaataatga catacagtga atttaagcac 1500

tgctgggaca cctttgtgga ccaccaggga tgtcccttcc agccctggga tggactagat 1560

gagcacagcc aagacctgag tgggaggctg cgggccattc tccagaatca ggaaaactct 1620

ggaggatcta gcggaggatc ctctggcagc gagacaccag gaacaagcga gtcagcaaca 1680

ccagagagca gtggcggcag cagcggcggc agcgacaaga agtacagcat cggcctggcc 1740

atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt gcccagcaag 1800

aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct gatcggagcc 1860

ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac cgccagaaga 1920

agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag caacgagatg 1980

gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt ggaagaggat 2040

aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc ctaccacgag 2100

aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga caaggccgac 2160

ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca cttcctgatc 2220

gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca gctggtgcag 2280

acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc 2340

atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc ccagctgccc 2400

ggcgagaaga agaatggcct gttcggaaac ctgattgccc tgagcctggg cctgaccccc 2460

aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag caaggacacc 2520

tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc cgacctgttt 2580

ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag agtgaacacc 2640

gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga gcaccaccag 2700

gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta caaagagatt 2760

ttcttcgacc agagcaagaa cggctacgcc ggctacattg acggcggagc cagccaggaa 2820

gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga ggaactgctc 2880

gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc 2940

ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga agatttttac 3000

ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg catcccctac 3060

tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag aaagagcgag 3120

gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgcttc cgcccagagc 3180

ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt gctgcccaag 3240

cacagcctgc tgtacgagta cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg 3300

accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc catcgtggac 3360

ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag 3420

aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc 3480

ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct ggacaatgag 3540

gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga ggacagagag 3600

atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt gatgaagcag 3660

ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat caacggcatc 3720

cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg cttcgccaac 3780

agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga catccagaaa 3840

gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct ggccggcagc 3900

cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg 3960

atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc 4020

cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg catcaaagag 4080

ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca gaacgagaag 4140

ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga actggacatc 4200

aaccggctgt ccgactacga tgtggaccat atcgtgcctc agagctttct gaaggacgac 4260

tccatcgaca acaaggtgct gaccagaagc gacaagaacc ggggcaagag cgacaacgtg 4320

ccctccgaag aggtcgtgaa gaagatgaag aactactggc ggcagctgct gaacgccaag 4380

ctgattaccc agagaaagtt cgacaatctg accaaggccg agagaggcgg cctgagcgaa 4440

ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat tacaaagcac 4500

gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaatga caagctgatc 4560

cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg gaaggatttc 4620

cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc ctacctaaac 4680

gccgtcgtgg gaaccgcact gatcaaaaag taccctaagc tggaaagcga gttcgtgtac 4740

ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca ggaaatcggc 4800

aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa gaccgagatt 4860

accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg 4920

gagatcgtgt gggataaggg ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc 4980

caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa agagtctatc 5040

agacccaaga ggaacagcga taagctgatc gccagaaaga aggactggga ccctaagaag 5100

tacggcggct tcgtgagccc caccgtggcc tattctgtgc tggtggtggc caaagtggaa 5160

aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac catcatggaa 5220

agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta caaagaagtg 5280

aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga aaacggccgg 5340

aagagaatgc tggcctctgc cagattcctg cagaagggaa acgaactggc cctgccctcc 5400

aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg ctcccccgag 5460

gataatgagc agaaacagct gtttgtggaa cagcacaagc actacctgga cgagatcatc 5520

gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct ggacaaagtg 5580

ctgtccgcct acaacaagca ccgggataag cccatcagag agcaggccga gaatatcatc 5640

cacctgttta ccctgaccaa tctgggagcc cctagagcct tcaagtactt tgacaccacc 5700

atcgaccgga aggtgtacag aagcaccaaa gaggtgctgg acgccaccct gatccaccag 5760

agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg tgacagcggc 5820

gggagcggcg ggagcggggg gagcactaat ctgagcgaca tcattgagaa ggagactggg 5880

aaacagctgg tcattcagga gtccatcctg atgctgcctg aggaggtgga ggaagtgatc 5940

ggcaacaagc cagagtctga catcctggtg cacaccgcct acgacgagtc cacagatgag 6000

aatgtgatgc tgctgacctc tgacgccccc gagtataagc cttgggccct ggtcatccag 6060

gattctaacg gcgagaataa gatcaagatg ctgagcggag gatccggagg atctggaggc 6120

agcaccaacc tgtctgacat catcgagaag gagacaggca agcagctggt catccaggag 6180

agcatcctga tgctgcccga agaagtcgaa gaagtgatcg gaaacaagcc tgagagcgat 6240

atcctggtcc ataccgccta cgacgagagt accgacgaaa atgtgatgct gctgacatcc 6300

gacgccccag agtataagcc ctgggctctg gtcatccagg attccaacgg agagaacaaa 6360

atcaaaatgc tgtctggcgg ctcaaaaaga accgccgacg gcagcgaatt cgagcccaag 6420

aagaagagga aagtctaacc ggtcatcatc accatcacca ttgagtttaa acccgctgat 6480

cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt 6540

ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat 6600

cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg 6660

gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg 6720

aggcggaaag aaccagctgg ggctcgatac cgtcgacctc tagctagagc ttggcgtaat 6780

catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 6840

gagccggaag cataaagtgt aaagcctagg atgcctaatg agtgagctaa ctcacattaa 6900

ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 6960

gaatcggcca acgcgcggga agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 7020

tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 7080

cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 7140

gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 7200

gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 7260

gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 7320

ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 7380

atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 7440

tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 7500

ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 7560

gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 7620

ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 7680

ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 7740

agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 7800

ggtctgacac tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 7860

aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 7920

tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 7980

cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 8040

tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 8100

cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 8160

ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 8220

gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 8280

gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 8340

gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 8400

gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 8460

tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 8520

aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 8580

cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 8640

caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 8700

cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 8760

ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 8820

aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 8880

tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 8940

tcgacggatc gggagatcga tctcccgatc ccctagggtc gactctcagt acaatctgct 9000

ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt 9060

agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga 9120

atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt 9180

gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 9240

catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 9300

acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 9360

ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 9420

aagtgtatc 9429

<210> 12

<211> 9429

<212> DNA

<213> Artificial Sequence

<220>

<223> 4M+P199A+P200K-BE4max

<400> 12

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca 420

gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcatgaa gcctcacttc 480

agaaacacag tggagcgaat gtatcgagac acattctcct acaactttta taatgcaccc 540

atcctttctc gtcggaatac cgtctggctg tgctacgaag tgaaaacaaa gggtccctca 600

aggccccctt tggacgcaaa gatctttcga ggccaggtgt attccgaact taagtaccac 660

ccagagatga gattcttcca ctggttcagc aagtggagga agctgcatcg tgaccaggag 720

tatgaggtca cctggtacat atccttgagc ccctgcacaa agtgtacaag ggatatggcc 780

acgttcctgg ccgaggaccc gaaggttacc ctgaccatct ttgttgcccg cctcgcctac 840

ttccttgacc cagattacca ggaggcgctt cgcagcctgt gtcagaaaag agacggtccg 900

cgtgccacca tgaagatcat gaattatgac gaatttcagc actgttggag caagttcgtg 960

tacagccaaa gagagctatt tgagccttgg aataatctgc ctaaatatta tatattactg 1020

cacatcatgc tgggggagat tctcagacac tcgatggatg ccaagacatt cactttcaac 1080

tttaacaatg aaccttgggt cagaggacgg catgagactt acctgtgtta tgaggtggag 1140

cgcatgcaca atgacacctg ggtcctgctg aaccagcgca ggggctttct atgcaaccag 1200

gctccacata aacacggttt ccttgaaggc cgccatgcag agctgtgctt cctggacgtg 1260

attccctttt ggaagctgga cctggaccag gactacaggg ttacctgctt cacctcctgg 1320

agcccctgct tcagctgtgc ccaggaaatg gctaaattca tttcaaaaaa caaacacgtg 1380

agcctgtgca tcttcactgc ccgcatctat gatgatcaag gaagatgtca ggaggggctg 1440

cgcaccctgg ccgaggctgg ggccaaaatt tcaataatga catacagtga atttaagcac 1500

tgctgggaca cctttgtgga ccaccaggga tgtcccttcc agccctggga tggactagat 1560

gagcacagcc aagacctgag tgggaggctg cgggccattc tccagaatca ggaaaactct 1620

ggaggatcta gcggaggatc ctctggcagc gagacaccag gaacaagcga gtcagcaaca 1680

ccagagagca gtggcggcag cagcggcggc agcgacaaga agtacagcat cggcctggcc 1740

atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt gcccagcaag 1800

aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct gatcggagcc 1860

ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac cgccagaaga 1920

agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag caacgagatg 1980

gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt ggaagaggat 2040

aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc ctaccacgag 2100

aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga caaggccgac 2160

ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca cttcctgatc 2220

gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca gctggtgcag 2280

acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc 2340

atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc ccagctgccc 2400

ggcgagaaga agaatggcct gttcggaaac ctgattgccc tgagcctggg cctgaccccc 2460

aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag caaggacacc 2520

tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc cgacctgttt 2580

ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag agtgaacacc 2640

gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga gcaccaccag 2700

gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta caaagagatt 2760

ttcttcgacc agagcaagaa cggctacgcc ggctacattg acggcggagc cagccaggaa 2820

gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga ggaactgctc 2880

gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc 2940

ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga agatttttac 3000

ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg catcccctac 3060

tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag aaagagcgag 3120

gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgcttc cgcccagagc 3180

ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt gctgcccaag 3240

cacagcctgc tgtacgagta cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg 3300

accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc catcgtggac 3360

ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag 3420

aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc 3480

ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct ggacaatgag 3540

gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga ggacagagag 3600

atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt gatgaagcag 3660

ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat caacggcatc 3720

cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg cttcgccaac 3780

agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga catccagaaa 3840

gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct ggccggcagc 3900

cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg 3960

atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc 4020

cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg catcaaagag 4080

ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca gaacgagaag 4140

ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga actggacatc 4200

aaccggctgt ccgactacga tgtggaccat atcgtgcctc agagctttct gaaggacgac 4260

tccatcgaca acaaggtgct gaccagaagc gacaagaacc ggggcaagag cgacaacgtg 4320

ccctccgaag aggtcgtgaa gaagatgaag aactactggc ggcagctgct gaacgccaag 4380

ctgattaccc agagaaagtt cgacaatctg accaaggccg agagaggcgg cctgagcgaa 4440

ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat tacaaagcac 4500

gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaatga caagctgatc 4560

cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg gaaggatttc 4620

cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc ctacctaaac 4680

gccgtcgtgg gaaccgcact gatcaaaaag taccctaagc tggaaagcga gttcgtgtac 4740

ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca ggaaatcggc 4800

aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa gaccgagatt 4860

accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg 4920

gagatcgtgt gggataaggg ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc 4980

caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa agagtctatc 5040

agacccaaga ggaacagcga taagctgatc gccagaaaga aggactggga ccctaagaag 5100

tacggcggct tcgtgagccc caccgtggcc tattctgtgc tggtggtggc caaagtggaa 5160

aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac catcatggaa 5220

agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta caaagaagtg 5280

aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga aaacggccgg 5340

aagagaatgc tggcctctgc cagattcctg cagaagggaa acgaactggc cctgccctcc 5400

aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg ctcccccgag 5460

gataatgagc agaaacagct gtttgtggaa cagcacaagc actacctgga cgagatcatc 5520

gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct ggacaaagtg 5580

ctgtccgcct acaacaagca ccgggataag cccatcagag agcaggccga gaatatcatc 5640

cacctgttta ccctgaccaa tctgggagcc cctagagcct tcaagtactt tgacaccacc 5700

atcgaccgga aggtgtacag aagcaccaaa gaggtgctgg acgccaccct gatccaccag 5760

agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg tgacagcggc 5820

gggagcggcg ggagcggggg gagcactaat ctgagcgaca tcattgagaa ggagactggg 5880

aaacagctgg tcattcagga gtccatcctg atgctgcctg aggaggtgga ggaagtgatc 5940

ggcaacaagc cagagtctga catcctggtg cacaccgcct acgacgagtc cacagatgag 6000

aatgtgatgc tgctgacctc tgacgccccc gagtataagc cttgggccct ggtcatccag 6060

gattctaacg gcgagaataa gatcaagatg ctgagcggag gatccggagg atctggaggc 6120

agcaccaacc tgtctgacat catcgagaag gagacaggca agcagctggt catccaggag 6180

agcatcctga tgctgcccga agaagtcgaa gaagtgatcg gaaacaagcc tgagagcgat 6240

atcctggtcc ataccgccta cgacgagagt accgacgaaa atgtgatgct gctgacatcc 6300

gacgccccag agtataagcc ctgggctctg gtcatccagg attccaacgg agagaacaaa 6360

atcaaaatgc tgtctggcgg ctcaaaaaga accgccgacg gcagcgaatt cgagcccaag 6420

aagaagagga aagtctaacc ggtcatcatc accatcacca ttgagtttaa acccgctgat 6480

cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt 6540

ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat 6600

cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg 6660

gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg 6720

aggcggaaag aaccagctgg ggctcgatac cgtcgacctc tagctagagc ttggcgtaat 6780

catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 6840

gagccggaag cataaagtgt aaagcctagg atgcctaatg agtgagctaa ctcacattaa 6900

ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 6960

gaatcggcca acgcgcggga agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 7020

tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 7080

cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 7140

gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 7200

gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 7260

gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 7320

ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 7380

atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 7440

tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 7500

ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 7560

gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 7620

ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 7680

ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 7740

agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 7800

ggtctgacac tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 7860

aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 7920

tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 7980

cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 8040

tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 8100

cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 8160

ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 8220

gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 8280

gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 8340

gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 8400

gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 8460

tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 8520

aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 8580

cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 8640

caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 8700

cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 8760

ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 8820

aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 8880

tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 8940

tcgacggatc gggagatcga tctcccgatc ccctagggtc gactctcagt acaatctgct 9000

ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt 9060

agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga 9120

atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt 9180

gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 9240

catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 9300

acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 9360

ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 9420

aagtgtatc 9429

<210> 13

<211> 9429

<212> DNA

<213> Artificial Sequence

<220>

<223> 4M+D128K+P199A+P200K-BE4max

<400> 13

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca 420

gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcatgaa gcctcacttc 480

agaaacacag tggagcgaat gtatcgagac acattctcct acaactttta taatgcaccc 540

atcctttctc gtcggaatac cgtctggctg tgctacgaag tgaaaacaaa gggtccctca 600

aggccccctt tggacgcaaa gatctttcga ggccaggtgt attccgaact taagtaccac 660

ccagagatga gattcttcca ctggttcagc aagtggagga agctgcatcg tgaccaggag 720

tatgaggtca cctggtacat atccttgagc ccctgcacaa agtgtacaag ggatatggcc 780

acgttcctgg ccgaggaccc gaaggttacc ctgaccatct ttgttgcccg cctcgcctac 840

ttccttaagc cagattacca ggaggcgctt cgcagcctgt gtcagaaaag agacggtccg 900

cgtgccacca tgaagatcat gaattatgac gaatttcagc actgttggag caagttcgtg 960

tacagccaaa gagagctatt tgagccttgg aataatctgc ctaaatatta tatattactg 1020

cacatcatgc tgggggagat tctcagacac tcgatggatg ccaagacatt cactttcaac 1080

tttaacaatg aaccttgggt cagaggacgg catgagactt acctgtgtta tgaggtggag 1140

cgcatgcaca atgacacctg ggtcctgctg aaccagcgca ggggctttct atgcaaccag 1200

gctccacata aacacggttt ccttgaaggc cgccatgcag agctgtgctt cctggacgtg 1260

attccctttt ggaagctgga cctggaccag gactacaggg ttacctgctt cacctcctgg 1320

agcccctgct tcagctgtgc ccaggaaatg gctaaattca tttcaaaaaa caaacacgtg 1380

agcctgtgca tcttcactgc ccgcatctat gatgatcaag gaagatgtca ggaggggctg 1440

cgcaccctgg ccgaggctgg ggccaaaatt tcaataatga catacagtga atttaagcac 1500

tgctgggaca cctttgtgga ccaccaggga tgtcccttcc agccctggga tggactagat 1560

gagcacagcc aagacctgag tgggaggctg cgggccattc tccagaatca ggaaaactct 1620

ggaggatcta gcggaggatc ctctggcagc gagacaccag gaacaagcga gtcagcaaca 1680

ccagagagca gtggcggcag cagcggcggc agcgacaaga agtacagcat cggcctggcc 1740

atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt gcccagcaag 1800

aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct gatcggagcc 1860

ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac cgccagaaga 1920

agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag caacgagatg 1980

gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt ggaagaggat 2040

aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc ctaccacgag 2100

aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga caaggccgac 2160

ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca cttcctgatc 2220

gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca gctggtgcag 2280

acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc 2340

atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc ccagctgccc 2400

ggcgagaaga agaatggcct gttcggaaac ctgattgccc tgagcctggg cctgaccccc 2460

aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag caaggacacc 2520

tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc cgacctgttt 2580

ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag agtgaacacc 2640

gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga gcaccaccag 2700

gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta caaagagatt 2760

ttcttcgacc agagcaagaa cggctacgcc ggctacattg acggcggagc cagccaggaa 2820

gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga ggaactgctc 2880

gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc 2940

ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga agatttttac 3000

ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg catcccctac 3060

tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag aaagagcgag 3120

gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgcttc cgcccagagc 3180

ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt gctgcccaag 3240

cacagcctgc tgtacgagta cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg 3300

accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc catcgtggac 3360

ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag 3420

aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc 3480

ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct ggacaatgag 3540

gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga ggacagagag 3600

atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt gatgaagcag 3660

ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat caacggcatc 3720

cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg cttcgccaac 3780

agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga catccagaaa 3840

gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct ggccggcagc 3900

cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg 3960

atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc 4020

cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg catcaaagag 4080

ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca gaacgagaag 4140

ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga actggacatc 4200

aaccggctgt ccgactacga tgtggaccat atcgtgcctc agagctttct gaaggacgac 4260

tccatcgaca acaaggtgct gaccagaagc gacaagaacc ggggcaagag cgacaacgtg 4320

ccctccgaag aggtcgtgaa gaagatgaag aactactggc ggcagctgct gaacgccaag 4380

ctgattaccc agagaaagtt cgacaatctg accaaggccg agagaggcgg cctgagcgaa 4440

ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat tacaaagcac 4500

gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaatga caagctgatc 4560

cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg gaaggatttc 4620

cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc ctacctaaac 4680

gccgtcgtgg gaaccgcact gatcaaaaag taccctaagc tggaaagcga gttcgtgtac 4740

ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca ggaaatcggc 4800

aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa gaccgagatt 4860

accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg 4920

gagatcgtgt gggataaggg ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc 4980

caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa agagtctatc 5040

agacccaaga ggaacagcga taagctgatc gccagaaaga aggactggga ccctaagaag 5100

tacggcggct tcgtgagccc caccgtggcc tattctgtgc tggtggtggc caaagtggaa 5160

aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac catcatggaa 5220

agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta caaagaagtg 5280

aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga aaacggccgg 5340

aagagaatgc tggcctctgc cagattcctg cagaagggaa acgaactggc cctgccctcc 5400

aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg ctcccccgag 5460

gataatgagc agaaacagct gtttgtggaa cagcacaagc actacctgga cgagatcatc 5520

gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct ggacaaagtg 5580

ctgtccgcct acaacaagca ccgggataag cccatcagag agcaggccga gaatatcatc 5640

cacctgttta ccctgaccaa tctgggagcc cctagagcct tcaagtactt tgacaccacc 5700

atcgaccgga aggtgtacag aagcaccaaa gaggtgctgg acgccaccct gatccaccag 5760

agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg tgacagcggc 5820

gggagcggcg ggagcggggg gagcactaat ctgagcgaca tcattgagaa ggagactggg 5880

aaacagctgg tcattcagga gtccatcctg atgctgcctg aggaggtgga ggaagtgatc 5940

ggcaacaagc cagagtctga catcctggtg cacaccgcct acgacgagtc cacagatgag 6000

aatgtgatgc tgctgacctc tgacgccccc gagtataagc cttgggccct ggtcatccag 6060

gattctaacg gcgagaataa gatcaagatg ctgagcggag gatccggagg atctggaggc 6120

agcaccaacc tgtctgacat catcgagaag gagacaggca agcagctggt catccaggag 6180

agcatcctga tgctgcccga agaagtcgaa gaagtgatcg gaaacaagcc tgagagcgat 6240

atcctggtcc ataccgccta cgacgagagt accgacgaaa atgtgatgct gctgacatcc 6300

gacgccccag agtataagcc ctgggctctg gtcatccagg attccaacgg agagaacaaa 6360

atcaaaatgc tgtctggcgg ctcaaaaaga accgccgacg gcagcgaatt cgagcccaag 6420

aagaagagga aagtctaacc ggtcatcatc accatcacca ttgagtttaa acccgctgat 6480

cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt 6540

ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat 6600

cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg 6660

gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg 6720

aggcggaaag aaccagctgg ggctcgatac cgtcgacctc tagctagagc ttggcgtaat 6780

catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 6840

gagccggaag cataaagtgt aaagcctagg atgcctaatg agtgagctaa ctcacattaa 6900

ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 6960

gaatcggcca acgcgcggga agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 7020

tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 7080

cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 7140

gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 7200

gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 7260

gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 7320

ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 7380

atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 7440

tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 7500

ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 7560

gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 7620

ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 7680

ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 7740

agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 7800

ggtctgacac tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 7860

aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 7920

tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 7980

cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 8040

tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 8100

cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 8160

ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 8220

gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 8280

gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 8340

gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 8400

gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 8460

tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 8520

aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 8580

cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 8640

caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 8700

cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 8760

ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 8820

aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 8880

tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 8940

tcgacggatc gggagatcga tctcccgatc ccctagggtc gactctcagt acaatctgct 9000

ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt 9060

agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga 9120

atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt 9180

gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 9240

catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 9300

acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 9360

ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 9420

aagtgtatc 9429

<210> 14

<211> 9429

<212> DNA

<213> Artificial Sequence

<220>

<223> A3G(OP)+P199A+P200K-BE4max

<400> 14

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca 420

gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcatgaa gccccacttt 480

cggaacaccg tggagcggat gtacagagat accttcagct acaacttcta taatagacct 540

atcctgtccc ggagaaatac cgtgtggctg tgctatgagg tgaagacaaa gggcccatct 600

cggccccctc tggatgccaa gatctttaga ggccaggtgt acagcgagct gaagtatcac 660

cctgagatga ggttctttca ctggttctcc aagtggagga agctgcaccg cgaccaggag 720

tacgaggtga cctggtatat cagctggtcc ccctgcacca agtgtacacg cgatatggcc 780

acatttctgg ccgaggaccc taaggtgacc ctgacaatct ttgtggccag gctgtactat 840

ttccgggacc cagattacca ggaggccctg cgctctctgt gccagaagcg ggatggcccc 900

agagccacca tgaagatcat gaactacgac gagtttcagc actgttggag caagttcgtg 960

tattcccagc gggagctgtt cgagccttgg aacaatctgc caaagtacta tatcctgctg 1020

cacatcatgc tgggcgagat cctgagacac agcatggatg ccaagacctt caccttcaac 1080

ttcaacaatg agccatgggt gcggggcaga cacgagacct acctgtgcta tgaggtggag 1140

cggatgcaca acgacacatg ggtgctgctg aatcagaggc gcggctttct gtgcaatcag 1200

gcaccacaca agcacggctt cctggagggc aggcacgcag agctgtgctt cctggatgtg 1260

atccctttct ggaagctgga cctggatcag gactaccgcg tgacctgttt tacatcttgg 1320

agcccatgct tctcctgtgc ccaggagatg gccaagttta tctccaagaa taagcacgtg 1380

tctctgtgca tcttcaccgc caggatctac gacgatcagg gcaggtgtca ggagggactg 1440

cgcacactgg cagaggcagg agccaagatc tctatcatga cctatagcga gtttaagcac 1500

tgctgggata cattcgtgga ccaccagggc tgtccattcc agccctggga tggcctggac 1560

gagcactccc aggacctgtc tggcaggctg agggccatcc tgcagaacca ggagaattct 1620

ggaggatcta gcggaggatc ctctggcagc gagacaccag gaacaagcga gtcagcaaca 1680

ccagagagca gtggcggcag cagcggcggc agcgacaaga agtacagcat cggcctggcc 1740

atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt gcccagcaag 1800

aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct gatcggagcc 1860

ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac cgccagaaga 1920

agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag caacgagatg 1980

gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt ggaagaggat 2040

aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc ctaccacgag 2100

aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga caaggccgac 2160

ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca cttcctgatc 2220

gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca gctggtgcag 2280

acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc 2340

atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc ccagctgccc 2400

ggcgagaaga agaatggcct gttcggaaac ctgattgccc tgagcctggg cctgaccccc 2460

aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag caaggacacc 2520

tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc cgacctgttt 2580

ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag agtgaacacc 2640

gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga gcaccaccag 2700

gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta caaagagatt 2760

ttcttcgacc agagcaagaa cggctacgcc ggctacattg acggcggagc cagccaggaa 2820

gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga ggaactgctc 2880

gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc 2940

ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga agatttttac 3000

ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg catcccctac 3060

tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag aaagagcgag 3120

gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgcttc cgcccagagc 3180

ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt gctgcccaag 3240

cacagcctgc tgtacgagta cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg 3300

accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc catcgtggac 3360

ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag 3420

aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc 3480

ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct ggacaatgag 3540

gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga ggacagagag 3600

atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt gatgaagcag 3660

ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat caacggcatc 3720

cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg cttcgccaac 3780

agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga catccagaaa 3840

gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct ggccggcagc 3900

cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg 3960

atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc 4020

cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg catcaaagag 4080

ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca gaacgagaag 4140

ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga actggacatc 4200

aaccggctgt ccgactacga tgtggaccat atcgtgcctc agagctttct gaaggacgac 4260

tccatcgaca acaaggtgct gaccagaagc gacaagaacc ggggcaagag cgacaacgtg 4320

ccctccgaag aggtcgtgaa gaagatgaag aactactggc ggcagctgct gaacgccaag 4380

ctgattaccc agagaaagtt cgacaatctg accaaggccg agagaggcgg cctgagcgaa 4440

ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat tacaaagcac 4500

gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaatga caagctgatc 4560

cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg gaaggatttc 4620

cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc ctacctaaac 4680

gccgtcgtgg gaaccgcact gatcaaaaag taccctaagc tggaaagcga gttcgtgtac 4740

ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca ggaaatcggc 4800

aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa gaccgagatt 4860

accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg 4920

gagatcgtgt gggataaggg ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc 4980

caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa agagtctatc 5040

agacccaaga ggaacagcga taagctgatc gccagaaaga aggactggga ccctaagaag 5100

tacggcggct tcgtgagccc caccgtggcc tattctgtgc tggtggtggc caaagtggaa 5160

aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac catcatggaa 5220

agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta caaagaagtg 5280

aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga aaacggccgg 5340

aagagaatgc tggcctctgc cagattcctg cagaagggaa acgaactggc cctgccctcc 5400

aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg ctcccccgag 5460

gataatgagc agaaacagct gtttgtggaa cagcacaagc actacctgga cgagatcatc 5520

gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct ggacaaagtg 5580

ctgtccgcct acaacaagca ccgggataag cccatcagag agcaggccga gaatatcatc 5640

cacctgttta ccctgaccaa tctgggagcc cctagagcct tcaagtactt tgacaccacc 5700

atcgaccgga aggtgtacag aagcaccaaa gaggtgctgg acgccaccct gatccaccag 5760

agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg tgacagcggc 5820

gggagcggcg ggagcggggg gagcactaat ctgagcgaca tcattgagaa ggagactggg 5880

aaacagctgg tcattcagga gtccatcctg atgctgcctg aggaggtgga ggaagtgatc 5940

ggcaacaagc cagagtctga catcctggtg cacaccgcct acgacgagtc cacagatgag 6000

aatgtgatgc tgctgacctc tgacgccccc gagtataagc cttgggccct ggtcatccag 6060

gattctaacg gcgagaataa gatcaagatg ctgagcggag gatccggagg atctggaggc 6120

agcaccaacc tgtctgacat catcgagaag gagacaggca agcagctggt catccaggag 6180

agcatcctga tgctgcccga agaagtcgaa gaagtgatcg gaaacaagcc tgagagcgat 6240

atcctggtcc ataccgccta cgacgagagt accgacgaaa atgtgatgct gctgacatcc 6300

gacgccccag agtataagcc ctgggctctg gtcatccagg attccaacgg agagaacaaa 6360

atcaaaatgc tgtctggcgg ctcaaaaaga accgccgacg gcagcgaatt cgagcccaag 6420

aagaagagga aagtctaacc ggtcatcatc accatcacca ttgagtttaa acccgctgat 6480

cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt 6540

ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat 6600

cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg 6660

gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg 6720

aggcggaaag aaccagctgg ggctcgatac cgtcgacctc tagctagagc ttggcgtaat 6780

catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 6840

gagccggaag cataaagtgt aaagcctagg atgcctaatg agtgagctaa ctcacattaa 6900

ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 6960

gaatcggcca acgcgcggga agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 7020

tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 7080

cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 7140

gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 7200

gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 7260

gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 7320

ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 7380

atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 7440

tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 7500

ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 7560

gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 7620

ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 7680

ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 7740

agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 7800

ggtctgacac tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 7860

aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 7920

tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 7980

cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 8040

tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 8100

cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 8160

ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 8220

gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 8280

gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 8340

gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 8400

gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 8460

tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 8520

aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 8580

cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 8640

caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 8700

cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 8760

ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 8820

aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 8880

tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 8940

tcgacggatc gggagatcga tctcccgatc ccctagggtc gactctcagt acaatctgct 9000

ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt 9060

agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga 9120

atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt 9180

gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 9240

catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 9300

acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 9360

ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 9420

aagtgtatc 9429

<210> 15

<211> 24

<212> DNA

<213> Artificial Sequence

<220>

<223> Polynucleotide sequences

<400> 15

accgggccca gactgagcac gtga 24

<210> 16

<211> 24

<212> DNA

<213> Artificial Sequence

<220>

<223> Polynucleotide sequences

<400> 16

aaactcacgt gctcagtctg ggcc 24

<210> 17

<211> 24

<212> DNA

<213> Artificial Sequence

<220>

<223> Polynucleotide sequences

<400> 17

accgtgcccc tccctccctg gccc 24

<210> 18

<211> 24

<212> DNA

<213> Artificial Sequence

<220>

<223> Polynucleotide sequences

<400> 18

aaacgggcca gggagggagg ggca 24

<210> 19

<211> 24

<212> DNA

<213> Artificial Sequence

<220>

<223> Polynucleotide sequences

<400> 19

accggaacac aaagcataga ctgc 24

<210> 20

<211> 24

<212> DNA

<213> Artificial Sequence

<220>

<223> Polynucleotide sequences

<400> 20

aaacgcagtc tatgctttgt gttc 24

<210> 21

<211> 27

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 21

gcccatgcaa ttagtctatt tctgctg 27

<210> 22

<211> 22

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 22

gcaggagctg cacatactag cc 22

<210> 23

<211> 22

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 23

ggggccccta accctatgta gc 22

<210> 24

<211> 20

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 24

ccattggcct gcttcgtggc 20

<210> 25

<211> 22

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 25

gttactgcag cccaagcctc ag 22

<210> 26

<211> 23

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 26

gtccagcccc atctgtcaaa ctg 23

<210> 27

<211> 583

<212> DNA

<213> APOBEC3G fragment

<400> 27

ggagattctc agacactcga tggatccaaa gacattcact ttcaacttta acaatgaacc 60

ttgggtcaga ggacggcatg agacttacct gtgttatgag gtggagcgca tgcacaatga 120

cacctgggtc ctgctgaacc agcgcagggg ctttctatgc aaccaggctc cacataaaca 180

cggtttcctt gaaggccgcc atgcagagct gtgcttcctg gacgtgattc ccttttggaa 240

gctggacctg gaccaggact acagggttac ctgcttcacc tcctggagcc cctgcttcag 300

ctgtgcccag gaaatggcta aattcatttc aaaaaacaaa cacgtgagcc tgtgcatctt 360

cactgcccgc atctatgatg atcaaggaag atgtcaggag gggctgcgca ccctggccga 420

ggctggggcc aaaatttcaa taatgacata cagtgaattt aagcactgct gggacacctt 480

tgtggaccac cagggatgtc ccttccagcc ctgggatgga ctagatgagc acagccaaga 540

cctgagtggg aggctgcggg ccattctcca gaatcaggaa aac 583

<210> 28

<211> 564

<212> DNA

<213> Artificial Sequence

<220>

<223> APOBEC3G fragment

<400> 28

atggatccaa agacattcac tttcaacttt aacaatgaac cttgggtcag aggacggcat 60

gagacttacc tgtgttatga ggtggagcgc atgcacaatg acacctgggt cctgctgaac 120

cagcgcaggg gctttctatg caaccaggct ccacataaac acggtttcct tgaaggccgc 180

catgcagagc tgtgcttcct ggacgtgatt cccttttgga agctggacct ggaccaggac 240

tacagggtta cctgcttcac ctcctggagc ccctgcttca gctgtgccca ggaaatggct 300

aaattcattt caaaaaacaa acacgtgagc ctgtgcatct tcactgcccg catctatgat 360

gatcaaggaa gatgtcagga ggggctgcgc accctggccg aggctggggc caaaatttca 420

ataatgacat acagtgaatt taagcactgc tgggacacct ttgtggacca ccagggatgt 480

cccttccagc cctgggatgg actagatgag cacagccaag acctgagtgg gaggctgcgg 540

gccattctcc agaatcagga aaac 564

<210> 29

<211> 1152

<212> DNA

<213> Artificial Sequence

<220>

<223> APOBEC3G fragment

<400> 29

atgaagcctc acttcagaaa cacagtggag cgaatgtatc gagacacatt ctcctacaac 60

ttttataatg cacccatcct ttctcgtcgg aataccgtct ggctgtgcta cgaagtgaaa 120

acaaagggtc cctcaaggcc ccctttggac gcaaagatct ttcgaggcca ggtgtattcc 180

gaacttaagt accacccaga gatgagattc ttccactggt tcagcaagtg gaggaagctg 240

catcgtgacc aggagtatga ggtcacctgg tacatatcct tgagcccctg cacaaagtgt 300

acaagggata tggccacgtt cctggccgag gacccgaagg ttaccctgac catctttgtt 360

gcccgcctcg cctacttcct tgacccagat taccaggagg cgcttcgcag cctgtgtcag 420

aaaagagacg gtccgcgtgc caccatgaag atcatgaatt atgacgaatt tcagcactgt 480

tggagcaagt tcgtgtacag ccaaagagag ctatttgagc cttggaataa tctgcctaaa 540

tattatatat tactgcacat catgctgggg gagattctca gacactcgat ggatccaccc 600

acattcactt tcaactttaa caatgaacct tgggtcagag gacggcatga gacttacctg 660

tgttatgagg tggagcgcat gcacaatgac acctgggtcc tgctgaacca gcgcaggggc 720

tttctatgca accaggctcc acataaacac ggtttccttg aaggccgcca tgcagagctg 780

tgcttcctgg acgtgattcc cttttggaag ctggacctgg accaggacta cagggttacc 840

tgcttcacct cctggagccc ctgcttcagc tgtgcccagg aaatggctaa attcatttca 900

aaaaacaaac acgtgagcct gtgcatcttc actgcccgca tctatgatga tcaaggaaga 960

tgtcaggagg ggctgcgcac cctggccgag gctggggcca aaatttcaat aatgacatac 1020

agtgaattta agcactgctg ggacaccttt gtggaccacc agggatgtcc cttccagccc 1080

tgggatggac tagatgagca cagccaagac ctgagtggga ggctgcgggc cattctccag 1140

aatcaggaaa ac 1152

<210> 30

<211> 1152

<212> DNA

<213> Artificial Sequence

<220>

<223> APOBEC3G fragment

<400> 30

atgaagcctc acttcagaaa cacagtggag cgaatgtatc gagacacatt ctcctacaac 60

ttttataatg cacccatcct ttctcgtcgg aataccgtct ggctgtgcta cgaagtgaaa 120

acaaagggtc cctcaaggcc ccctttggac gcaaagatct ttcgaggcca ggtgtattcc 180

gaacttaagt accacccaga gatgagattc ttccactggt tcagcaagtg gaggaagctg 240

catcgtgacc aggagtatga ggtcacctgg tacatatcct tgagcccctg cacaaagtgt 300

acaagggata tggccacgtt cctggccgag gacccgaagg ttaccctgac catctttgtt 360

gcccgcctcg cctacttcct taagccagat taccaggagg cgcttcgcag cctgtgtcag 420

aaaagagacg gtccgcgtgc caccatgaag atcatgaatt atgacgaatt tcagcactgt 480

tggagcaagt tcgtgtacag ccaaagagag ctatttgagc cttggaataa tctgcctaaa 540

tattatatat tactgcacat catgctgggg gagattctca gacactcgat ggatccaccc 600

acattcactt tcaactttaa caatgaacct tgggtcagag gacggcatga gacttacctg 660

tgttatgagg tggagcgcat gcacaatgac acctgggtcc tgctgaacca gcgcaggggc 720

tttctatgca accaggctcc acataaacac ggtttccttg aaggccgcca tgcagagctg 780

tgcttcctgg acgtgattcc cttttggaag ctggacctgg accaggacta cagggttacc 840

tgcttcacct cctggagccc ctgcttcagc tgtgcccagg aaatggctaa attcatttca 900

aaaaacaaac acgtgagcct gtgcatcttc actgcccgca tctatgatga tcaaggaaga 960

tgtcaggagg ggctgcgcac cctggccgag gctggggcca aaatttcaat aatgacatac 1020

agtgaattta agcactgctg ggacaccttt gtggaccacc agggatgtcc cttccagccc 1080

tgggatggac tagatgagca cagccaagac ctgagtggga ggctgcgggc cattctccag 1140

aatcaggaaa ac 1152

<210> 31

<211> 1152

<212> DNA

<213> Artificial Sequence

<220>

<223> APOBEC3G fragment

<400> 31

atgaagcctc acttcagaaa cacagtggag cgaatgtatc gagacacatt ctcctacaac 60

ttttataatg cacccatcct ttctcgtcgg aataccgtct ggctgtgcta cgaagtgaaa 120

acaaagggtc cctcaaggcc ccctttggac gcaaagatct ttcgaggcca ggtgtattcc 180

gaacttaagt accacccaga gatgagattc ttccactggt tcagcaagtg gaggaagctg 240

catcgtgacc aggagtatga ggtcacctgg tacatatcct tgagcccctg cacaaagtgt 300

acaagggata tggccacgtt cctggccgag gacccgaagg ttaccctgac catctttgtt 360

gcccgcctcg cctacttcct tgacccagat taccaggagg cgcttcgcag cctgtgtcag 420

aaaagagacg gtccgcgtgc caccatgaag atcatgaatt atgacgaatt tcagcactgt 480

tggagcaagt tcgtgtacag ccaaagagag ctatttgagc cttggaataa tctgcctaaa 540

tattatatat tactgcacat catgctgggg gagattctca gacactcgat ggatgccccc 600

acattcactt tcaactttaa caatgaacct tgggtcagag gacggcatga gacttacctg 660

tgttatgagg tggagcgcat gcacaatgac acctgggtcc tgctgaacca gcgcaggggc 720

tttctatgca accaggctcc acataaacac ggtttccttg aaggccgcca tgcagagctg 780

tgcttcctgg acgtgattcc cttttggaag ctggacctgg accaggacta cagggttacc 840

tgcttcacct cctggagccc ctgcttcagc tgtgcccagg aaatggctaa attcatttca 900

aaaaacaaac acgtgagcct gtgcatcttc actgcccgca tctatgatga tcaaggaaga 960

tgtcaggagg ggctgcgcac cctggccgag gctggggcca aaatttcaat aatgacatac 1020

agtgaattta agcactgctg ggacaccttt gtggaccacc agggatgtcc cttccagccc 1080

tgggatggac tagatgagca cagccaagac ctgagtggga ggctgcgggc cattctccag 1140

aatcaggaaa ac 1152

<210> 32

<211> 1152

<212> DNA

<213> Artificial Sequence

<220>

<223> APOBEC3G fragment

<400> 32

atgaagcctc acttcagaaa cacagtggag cgaatgtatc gagacacatt ctcctacaac 60

ttttataatg cacccatcct ttctcgtcgg aataccgtct ggctgtgcta cgaagtgaaa 120

acaaagggtc cctcaaggcc ccctttggac gcaaagatct ttcgaggcca ggtgtattcc 180

gaacttaagt accacccaga gatgagattc ttccactggt tcagcaagtg gaggaagctg 240

catcgtgacc aggagtatga ggtcacctgg tacatatcct tgagcccctg cacaaagtgt 300

acaagggata tggccacgtt cctggccgag gacccgaagg ttaccctgac catctttgtt 360

gcccgcctcg cctacttcct tgacccagat taccaggagg cgcttcgcag cctgtgtcag 420

aaaagagacg gtccgcgtgc caccatgaag atcatgaatt atgacgaatt tcagcactgt 480

tggagcaagt tcgtgtacag ccaaagagag ctatttgagc cttggaataa tctgcctaaa 540

tattatatat tactgcacat catgctgggg gagattctca gacactcgat ggattggccc 600

acattcactt tcaactttaa caatgaacct tgggtcagag gacggcatga gacttacctg 660

tgttatgagg tggagcgcat gcacaatgac acctgggtcc tgctgaacca gcgcaggggc 720

tttctatgca accaggctcc acataaacac ggtttccttg aaggccgcca tgcagagctg 780

tgcttcctgg acgtgattcc cttttggaag ctggacctgg accaggacta cagggttacc 840

tgcttcacct cctggagccc ctgcttcagc tgtgcccagg aaatggctaa attcatttca 900

aaaaacaaac acgtgagcct gtgcatcttc actgcccgca tctatgatga tcaaggaaga 960

tgtcaggagg ggctgcgcac cctggccgag gctggggcca aaatttcaat aatgacatac 1020

agtgaattta agcactgctg ggacaccttt gtggaccacc agggatgtcc cttccagccc 1080

tgggatggac tagatgagca cagccaagac ctgagtggga ggctgcgggc cattctccag 1140

aatcaggaaa ac 1152

<210> 33

<211> 1152

<212> DNA

<213> Artificial Sequence

<220>

<223> APOBEC3G fragment

<400> 33

atgaagcctc acttcagaaa cacagtggag cgaatgtatc gagacacatt ctcctacaac 60

ttttataatg cacccatcct ttctcgtcgg aataccgtct ggctgtgcta cgaagtgaaa 120

acaaagggtc cctcaaggcc ccctttggac gcaaagatct ttcgaggcca ggtgtattcc 180

gaacttaagt accacccaga gatgagattc ttccactggt tcagcaagtg gaggaagctg 240

catcgtgacc aggagtatga ggtcacctgg tacatatcct tgagcccctg cacaaagtgt 300

acaagggata tggccacgtt cctggccgag gacccgaagg ttaccctgac catctttgtt 360

gcccgcctcg cctacttcct tgacccagat taccaggagg cgcttcgcag cctgtgtcag 420

aaaagagacg gtccgcgtgc caccatgaag atcatgaatt atgacgaatt tcagcactgt 480

tggagcaagt tcgtgtacag ccaaagagag ctatttgagc cttggaataa tctgcctaaa 540

tattatatat tactgcacat catgctgggg gagattctca gacactcgat ggatccagcc 600

acattcactt tcaactttaa caatgaacct tgggtcagag gacggcatga gacttacctg 660

tgttatgagg tggagcgcat gcacaatgac acctgggtcc tgctgaacca gcgcaggggc 720

tttctatgca accaggctcc acataaacac ggtttccttg aaggccgcca tgcagagctg 780

tgcttcctgg acgtgattcc cttttggaag ctggacctgg accaggacta cagggttacc 840

tgcttcacct cctggagccc ctgcttcagc tgtgcccagg aaatggctaa attcatttca 900

aaaaacaaac acgtgagcct gtgcatcttc actgcccgca tctatgatga tcaaggaaga 960

tgtcaggagg ggctgcgcac cctggccgag gctggggcca aaatttcaat aatgacatac 1020

agtgaattta agcactgctg ggacaccttt gtggaccacc agggatgtcc cttccagccc 1080

tgggatggac tagatgagca cagccaagac ctgagtggga ggctgcgggc cattctccag 1140

aatcaggaaa ac 1152

<210> 34

<211> 1152

<212> DNA

<213> Artificial Sequence

<220>

<223> APOBEC3G fragment

<400> 34

atgaagcctc acttcagaaa cacagtggag cgaatgtatc gagacacatt ctcctacaac 60

ttttataatg cacccatcct ttctcgtcgg aataccgtct ggctgtgcta cgaagtgaaa 120

acaaagggtc cctcaaggcc ccctttggac gcaaagatct ttcgaggcca ggtgtattcc 180

gaacttaagt accacccaga gatgagattc ttccactggt tcagcaagtg gaggaagctg 240

catcgtgacc aggagtatga ggtcacctgg tacatatcct tgagcccctg cacaaagtgt 300

acaagggata tggccacgtt cctggccgag gacccgaagg ttaccctgac catctttgtt 360

gcccgcctcg cctacttcct tgacccagat taccaggagg cgcttcgcag cctgtgtcag 420

aaaagagacg gtccgcgtgc caccatgaag atcatgaatt atgacgaatt tcagcactgt 480

tggagcaagt tcgtgtacag ccaaagagag ctatttgagc cttggaataa tctgcctaaa 540

tattatatat tactgcacat catgctgggg gagattctca gacactcgat ggatccaaag 600

acattcactt tcaactttaa caatgaacct tgggtcagag gacggcatga gacttacctg 660

tgttatgagg tggagcgcat gcacaatgac acctgggtcc tgctgaacca gcgcaggggc 720

tttctatgca accaggctcc acataaacac ggtttccttg aaggccgcca tgcagagctg 780

tgcttcctgg acgtgattcc cttttggaag ctggacctgg accaggacta cagggttacc 840

tgcttcacct cctggagccc ctgcttcagc tgtgcccagg aaatggctaa attcatttca 900

aaaaacaaac acgtgagcct gtgcatcttc actgcccgca tctatgatga tcaaggaaga 960

tgtcaggagg ggctgcgcac cctggccgag gctggggcca aaatttcaat aatgacatac 1020

agtgaattta agcactgctg ggacaccttt gtggaccacc agggatgtcc cttccagccc 1080

tgggatggac tagatgagca cagccaagac ctgagtggga ggctgcgggc cattctccag 1140

aatcaggaaa ac 1152

<210> 35

<211> 1152

<212> DNA

<213> Artificial Sequence

<220>

<223> APOBEC3G fragment

<400> 35

atgaagcctc acttcagaaa cacagtggag cgaatgtatc gagacacatt ctcctacaac 60

ttttataatg cacccatcct ttctcgtcgg aataccgtct ggctgtgcta cgaagtgaaa 120

acaaagggtc cctcaaggcc ccctttggac gcaaagatct ttcgaggcca ggtgtattcc 180

gaacttaagt accacccaga gatgagattc ttccactggt tcagcaagtg gaggaagctg 240

catcgtgacc aggagtatga ggtcacctgg tacatatcct tgagcccctg cacaaagtgt 300

acaagggata tggccacgtt cctggccgag gacccgaagg ttaccctgac catctttgtt 360

gcccgcctcg cctacttcct tgacccagat taccaggagg cgcttcgcag cctgtgtcag 420

aaaagagacg gtccgcgtgc caccatgaag atcatgaatt atgacgaatt tcagcactgt 480

tggagcaagt tcgtgtacag ccaaagagag ctatttgagc cttggaataa tctgcctaaa 540

tattatatat tactgcacat catgctgggg gagattctca gacactcgat ggatccaccc 600

acattcactt tcaactttaa caatgaacct tgggtcagag gacggcatga gacttacctg 660

tgttatgagg tggagcgcat gcacaatgac acctgggtcc tgctgaacca gcgcaggggc 720

tttctatgca accaggctcc acataaacac ggtttccttg aaggccgcca tgcagagctg 780

tgcttcctgg acgtgattcc cttttggaag ctggacctgg accaggacta cagggttacc 840

tgcttcacct cctggagccc ctgcttcagc tgtgcccagg aaatggctaa attcatttca 900

aaaaacaaac acgtgagcct gtgcatcttc actgcccgca tctatgatga tcaaggaaga 960

tgtaaggagg ggctgcgcac cctggccgag gctggggcca aaatttcaat aatgacatac 1020

agtgaattta agcactgctg ggacaccttt gtggaccacc agggatgtcc cttccagccc 1080

tgggatggac tagatgagca cagccaagac ctgagtggga ggctgcgggc cattctccag 1140

aatcaggaaa ac 1152

<210> 36

<211> 1152

<212> DNA

<213> Artificial Sequence

<220>

<223> APOBEC3G fragment

<400> 36

atgaagcctc acttcagaaa cacagtggag cgaatgtatc gagacacatt ctcctacaac 60

ttttataatg cacccatcct ttctcgtcgg aataccgtct ggctgtgcta cgaagtgaaa 120

acaaagggtc cctcaaggcc ccctttggac gcaaagatct ttcgaggcca ggtgtattcc 180

gaacttaagt accacccaga gatgagattc ttccactggt tcagcaagtg gaggaagctg 240

catcgtgacc aggagtatga ggtcacctgg tacatatcct tgagcccctg cacaaagtgt 300

acaagggata tggccacgtt cctggccgag gacccgaagg ttaccctgac catctttgtt 360

gcccgcctcg cctacttcct taagccagat taccaggagg cgcttcgcag cctgtgtcag 420

aaaagagacg gtccgcgtgc caccatgaag atcatgaatt atgacgaatt tcagcactgt 480

tggagcaagt tcgtgtacag ccaaagagag ctatttgagc cttggaataa tctgcctaaa 540

tattatatat tactgcacat catgctgggg gagattctca gacactcgat ggatgccaag 600

acattcactt tcaactttaa caatgaacct tgggtcagag gacggcatga gacttacctg 660

tgttatgagg tggagcgcat gcacaatgac acctgggtcc tgctgaacca gcgcaggggc 720

tttctatgca accaggctcc acataaacac ggtttccttg aaggccgcca tgcagagctg 780

tgcttcctgg acgtgattcc cttttggaag ctggacctgg accaggacta cagggttacc 840

tgcttcacct cctggagccc ctgcttcagc tgtgcccagg aaatggctaa attcatttca 900

aaaaacaaac acgtgagcct gtgcatcttc actgcccgca tctatgatga tcaaggaaga 960

tgtcaggagg ggctgcgcac cctggccgag gctggggcca aaatttcaat aatgacatac 1020

agtgaattta agcactgctg ggacaccttt gtggaccacc agggatgtcc cttccagccc 1080

tgggatggac tagatgagca cagccaagac ctgagtggga ggctgcgggc cattctccag 1140

aatcaggaaa ac 1152

<210> 37

<211> 4101

<212> DNA

<213> Artificial Sequence

<220>

<223> SpCas9-D10A nickase fragment

<400> 37

gataaaaagt attctattgg tttagccatc ggcactaatt ccgttggatg ggctgtcata 60

accgatgaat acaaagtacc ttcaaagaaa tttaaggtgt tggggaacac agaccgtcat 120

tcgattaaaa agaatcttat cggtgccctc ctattcgata gtggcgaaac ggcagaggcg 180

actcgcctga aacgaaccgc tcggagaagg tatacacgtc gcaagaaccg aatatgttac 240

ttacaagaaa tttttagcaa tgagatggcc aaagttgacg attctttctt tcaccgtttg 300

gaagagtcct tccttgtcga agaggacaag aaacatgaac ggcaccccat ctttggaaac 360

atagtagatg aggtggcata tcatgaaaag tacccaacga tttatcacct cagaaaaaag 420

ctagttgact caactgataa agcggacctg aggttaatct acttggctct tgcccatatg 480

ataaagttcc gtgggcactt tctcattgag ggtgatctaa atccggacaa ctcggatgtc 540

gacaaactgt tcatccagtt agtacaaacc tataatcagt tgtttgaaga gaaccctata 600

aatgcaagtg gcgtggatgc gaaggctatt cttagcgccc gcctctctaa atcccgacgg 660

ctagaaaacc tgatcgcaca attacccgga gagaagaaaa atgggttgtt cggtaacctt 720

atagcgctct cactaggcct gacaccaaat tttaagtcga acttcgactt agctgaagat 780

gccaaattgc agcttagtaa ggacacgtac gatgacgatc tcgacaatct actggcacaa 840

attggagatc agtatgcgga cttatttttg gctgccaaaa accttagcga tgcaatcctc 900

ctatctgaca tactgagagt taatactgag attaccaagg cgccgttatc cgcttcaatg 960

atcaaaaggt acgatgaaca tcaccaagac ttgacacttc tcaaggccct agtccgtcag 1020

caactgcctg agaaatataa ggaaatattc tttgatcagt cgaaaaacgg gtacgcaggt 1080

tatattgacg gcggagcgag tcaagaggaa ttctacaagt ttatcaaacc catattagag 1140

aagatggatg ggacggaaga gttgcttgta aaactcaatc gcgaagatct actgcgaaag 1200

cagcggactt tcgacaacgg tagcattcca catcaaatcc acttaggcga attgcatgct 1260

atacttagaa ggcaggagga tttttatccg ttcctcaaag acaatcgtga aaagattgag 1320

aaaatcctaa cctttcgcat accttactat gtgggacccc tggcccgagg gaactctcgg 1380

ttcgcatgga tgacaagaaa gtccgaagaa acgattactc catggaattt tgaggaagtt 1440

gtcgataaag gtgcgtcagc tcaatcgttc atcgagagga tgaccaactt tgacaagaat 1500

ttaccgaacg aaaaagtatt gcctaagcac agtttacttt acgagtattt cacagtgtac 1560

aatgaactca cgaaagttaa gtatgtcact gagggcatgc gtaaacccgc ctttctaagc 1620

ggagaacaga agaaagcaat agtagatctg ttattcaaga ccaaccgcaa agtgacagtt 1680

aagcaattga aagaggacta ctttaagaaa attgaatgct tcgattctgt cgagatctcc 1740

ggggtagaag atcgatttaa tgcgtcactt ggtacgtatc atgacctcct aaagataatt 1800

aaagataagg acttcctgga taacgaagag aatgaagata tcttagaaga tatagtgttg 1860

actcttaccc tctttgaaga tcgggaaatg attgaggaaa gactaaaaac atacgctcac 1920

ctgttcgacg ataaggttat gaaacagtta aagaggcgtc gctatacggg ctggggacga 1980

ttgtcgcgga aacttatcaa cgggataaga gacaagcaaa gtggtaaaac tattctcgat 2040

tttctaaaga gcgacggctt cgccaatagg aactttatgc agctgatcca tgatgactct 2100

ttaaccttca aagaggatat acaaaaggca caggtttccg gacaagggga ctcattgcac 2160

gaacatattg cgaatcttgc tggttcgcca gccatcaaaa agggcatact ccagacagtc 2220

aaagtagtgg atgagctagt taaggtcatg ggacgtcaca aaccggaaaa cattgtaatc 2280

gagatggcac gcgaaaatca aacgactcag aaggggcaaa aaaacagtcg agagcggatg 2340

aagagaatag aagagggtat taaagaactg ggcagccaga tcttaaagga gcatcctgtg 2400

gaaaataccc aattgcagaa cgagaaactt tacctctatt acctacaaaa tggaagggac 2460

atgtatgttg atcaggaact ggacataaac cgtttatctg attacgacgt cgatcacatt 2520

gtaccccaat cctttttgaa ggacgattca atcgacaata aagtgcttac acgctcggat 2580

aagaaccgag ggaaaagtga caatgttcca agcgaggaag tcgtaaagaa aatgaagaac 2640

tattggcggc agctcctaaa tgcgaaactg ataacgcaaa gaaagttcga taacttaact 2700

aaagctgaga ggggtggctt gtctgaactt gacaaggccg gatttattaa acgtcagctc 2760

gtggaaaccc gccaaatcac aaagcatgtt gcacagatac tagattcccg aatgaatacg 2820

aaatacgacg agaacgataa gctgattcgg gaagtcaaag taatcacttt aaagtcaaaa 2880

ttggtgtcgg acttcagaaa ggattttcaa ttctataaag ttagggagat aaataactac 2940

caccatgcgc acgacgctta tcttaatgcc gtcgtaggga ccgcactcat taagaaatac 3000

ccgaagctag aaagtgagtt tgtgtatggt gattacaaag tttatgacgt ccgtaagatg 3060

atcgcgaaaa gcgaacagga gataggcaag gctacagcca aatacttctt ttattctaac 3120

attatgaatt tctttaagac ggaaatcact ctggcaaacg gagagatacg caaacgacct 3180

ttaattgaaa ccaatgggga gacaggtgaa atcgtatggg ataagggccg ggacttcgcg 3240

acggtgagaa aagttttgtc catgccccaa gtcaacatag taaagaaaac tgaggtgcag 3300

accggagggt tttcaaagga atcgattctt ccaaaaagga atagtgataa gctcatcgct 3360

cgtaaaaagg actgggaccc gaaaaagtac ggtggcttcg atagccctac agttgcctat 3420

tctgtcctag tagtggcaaa agttgagaag ggaaaatcca agaaactgaa gtcagtcaaa 3480

gaattattgg ggataacgat tatggagcgc tcgtcttttg aaaagaaccc catcgacttc 3540

cttgaggcga aaggttacaa ggaagtaaaa aaggatctca taattaaact accaaagtat 3600

agtctgtttg agttagaaaa tggccgaaaa cggatgttgg ctagcgccgg agagcttcaa 3660

aaggggaacg aactcgcact accgtctaaa tacgtgaatt tcctgtattt agcgtcccat 3720

tacgagaagt tgaaaggttc acctgaagat aacgaacaga agcaactttt tgttgagcag 3780

cacaaacatt atctcgacga aatcatagag caaatttcgg aattcagtaa gagagtcatc 3840

ctagctgatg ccaatctgga caaagtatta agcgcataca acaagcacag ggataaaccc 3900

atacgtgagc aggcggaaaa tattatccat ttgtttactc ttaccaacct cggcgctcca 3960

gccgcattca agtattttga cacaacgata gatcgcaaac gatacacttc taccaaggag 4020

gtgctagacg cgacactgat tcaccaatcc atcacgggat tatatgaaac tcggatagat 4080

ttgtcacagc ttgggggtga c 4101

<210> 38

<211> 4101

<212> DNA

<213> Artificial Sequence

<220>

<223> SpCas9-D10A nickase fragment

<400> 38

gacaagaagt acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc 60

accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac 120

agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc 180

acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat 240

ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg 300

gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac 360

atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa 420

ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg 480

atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg 540

gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc 600

aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg 660

ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg 720

attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat 780

gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag 840

atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg 900

ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg 960

atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag 1020

cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc 1080

tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa 1140

aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag 1200

cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc 1260

attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag 1320

aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga 1380

ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg 1440

gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac 1500

ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat 1560

aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc 1620

ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg 1680

aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc 1740

ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc 1800

aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg 1860

accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac 1920

ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg 1980

ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat 2040

ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc 2100

ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac 2160

gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg 2220

aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc 2280

gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg 2340

aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg 2400

gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat 2460

atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc 2520

gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac 2580

aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac 2640

tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc 2700

aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg 2760

gtggaaaccc ggcagattac aaagcacgtg gcacagatcc tggactcccg gatgaacact 2820

aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag 2880

ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac 2940

caccacgccc acgacgccta cctaaacgcc gtcgtgggaa ccgcactgat caaaaagtac 3000

cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg 3060

atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac 3120

atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct 3180

ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc 3240

accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag 3300

acaggcggct tcagcaaaga gtctatcaga cccaagagga acagcgataa gctgatcgcc 3360

agaaagaagg actgggaccc taagaagtac ggcggcttcg tgagccccac cgtggcctat 3420

tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa 3480

gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt 3540

ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac 3600

tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccag attcctgcag 3660

aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac 3720

tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag 3780

cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc 3840

ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc 3900

atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct 3960

agagccttca agtactttga caccaccatc gaccggaagg tgtacagaag caccaaagag 4020

gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac 4080

ctgtctcagc tgggaggtga c 4101

<210> 39

<211> 57

<212> DNA

<213> Artificial Sequence

<220>

<223> Nuclear localization Signal fragment

<400> 39

atgaaacgga cagccgacgg aagcgagttc gagtcaccaa agaagaagcg gaaagtc 57

<210> 40

<211> 96

<212> DNA

<213> Artificial Sequence

<220>

<223> Flexible linker peptide fragment

<400> 40

tctggaggat ctagcggagg atcctctgga agcgagacac caggcacaag cgagtccgcc 60

acaccagaga gctccggcgg ctcctccgga ggatcc 96

<210> 41

<211> 96

<212> DNA

<213> Artificial Sequence

<220>

<223> flexibly linked peptide fragment

<400> 41

tctggaggat ctagcggagg atcctctgga agcgagacac caggcacaag cgagtccgcc 60

acaccagaga gctccggcgg ctcctccgga ggatcc 96

Claims (9)

1. A fusion protein, wherein said fusion protein is encoded by a polynucleotide, and the sequence of said polynucleotide is set forth in any one of SEQ ID NO.3, 12, 13 or 14.

2. A construct constructed by inserting the polynucleotide of claim 1 into an expression vector; the polynucleotide sequence of the construct is shown in any one of SEQ ID NO.3, 12, 13 or 14.

3. The construct of claim 2, wherein the expression vector is one of a pCMV expression vector, a pSV2 expression vector, or a pGL3 expression vector.

4. An expression system which is a host cell comprising the construct of claim 2 or having the polynucleotide of claim 1 integrated into its genome.

5. The expression system of claim 4, wherein the host cell is selected from a mouse cell or a human cell.

6. The expression system of claim 4, wherein the host cell is selected from the group consisting of mouse brain neuroma cells, human embryonic kidney cells, human cervical cancer cells, human colon cancer cells, human osteosarcoma cells.

7. A base editing tool comprising the fusion protein of claim 1 and a sgRNA.

8. Use of the base editing tool of claim 7 in gene editing in eukaryotes.

9. The use of the base editing tool of claim 7 in eukaryotic gene editing, wherein the gene editing is base editing of C-to-T at positions 4-7 of the 5' end within the sgRNA target region.

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