CN110734864A - Curvularia lunata and application thereof - Google Patents
Curvularia lunata and application thereof Download PDFInfo
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- CN110734864A CN110734864A CN201911269657.7A CN201911269657A CN110734864A CN 110734864 A CN110734864 A CN 110734864A CN 201911269657 A CN201911269657 A CN 201911269657A CN 110734864 A CN110734864 A CN 110734864A
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- hexavalent chromium
- curvularia lunata
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
- C02F2101/22—Chromium or chromium compounds, e.g. chromates
Abstract
The invention discloses Curvularia lunata and application thereof in chromium-containing wastewater treatment, which is Curvularia lunata HT02, wherein the strain is stored in the common microorganism center of China Committee for culture Collection of microorganisms with the preservation number of CGMCC NO. 17597. strains with hexavalent chromium reduction capability are obtained by collecting a water sample of a steel mill wastewater storage tank, domesticating, screening, separating and purifying, and identified as Curvularia lunata with the self-coding number of HT02. the strain has fixed reduction treatment capability on chromium-containing wastewater, particularly has better and obvious effect on low-concentration hexavalent chromium wastewater, and has fixed application prospect and value in the aspect of microbial remediation of water polluted by low-concentration hexavalent chromium.
Description
Technical Field
The invention relates to the technical field of environmental microbial remediation, in particular to Curvularia lunata and application thereof.
Background
With the development of modern industrial economic technology, chromium salt series products are widely applied to industries such as electroplating, steel, electroplating, leather manufacturing and the like, however, due to the non-standardization of the industrial industry and the unsupervised force of a relevant part , a large amount of chromium-containing wastewater and waste residues are discharged and discarded randomly, and serious pollution is caused to the environment.
In recent years, a plurality of researchers write patents about the reduction of hexavalent chromium by microorganisms of different species, such as alcaligenes gtm2(CN201610426026.1), microbacterium celluliginum sp.gtm1(CN201610399636.7), Streptomyces puleus strain LYzzm (CN201610077641.6), Ochrobactrum ochraceum sp.cts-325(CN200710009694.5), Bacillus sp.y04), Bacillus bacterium strain 357, enterobacter strain 2(CN 358625), and the like, so that the high-efficiency reduction capability of hexavalent chromium (CN 2) and the high-efficiency reduction capability of hexavalent chromium (CN 3550) are necessary for the reduction of hexavalent chromium (CN 3550) and the high-efficiency reduction capability of hexavalent chromium (CN 3550/2) and the high-efficiency reduction capability of hexavalent chromium (CN 3550/3550) are necessary for the reduction of hexavalent chromium (CN 3645).
Disclosure of Invention
In view of the above, the present invention aims to provide Curvularia lunata and applications thereof, particularly applications to treatment of low-concentration hexavalent chromium wastewater.
In order to achieve the above object, Curvularia lunata of the present invention, which strain is Latin under the name Curvularia unaataHT 02, depository unit: china general microbiological culture Collection center, preservation date: year 2019, month 4 and day 15, accession number: CGMCC NO. 17597.
To achieve the above object, the microbial agent of the present invention comprises Curvularia lunata as set forth in claim 1.
, the application of the microbial inoculum in preparing and treating hexavalent chromium-containing wastewater products.
, the application of the microbial inoculum in treating the wastewater containing hexavalent chromium.
, the microbial inoculum is applied to the preparation of wastewater products containing low-concentration hexavalent chromium.
, the application of the microbial inoculum in treating wastewater containing low-concentration hexavalent chromium.
In order to achieve the above object, the method for treating wastewater containing hexavalent chromium of the present invention treats wastewater containing hexavalent chromium with the above Curvularia lunata or its bacterial suspension.
In some embodiments of the invention, Cr is included6+Are all prepared by adding potassium dichromate (K)2Cr2O7) Obtaining;
in the embodiment of the invention, the hexavalent chromium is measured by a dibenzoyl dihydrazide spectrophotometric method for measuring the hexavalent chromium in GB-T7467-87 water.
In the examples of the present invention, the removal rate of hexavalent chromium in a water sample is (initial concentration of hexavalent chromium-concentration of hexavalent chromium after treatment) ÷ initial concentration of hexavalent chromium × 100%.
Compared with the prior art, the invention has the following beneficial advantages:
the Curvularia lunata HT02 obtained by screening has certain reduction treatment effect on hexavalent chromium wastewater, particularly has better and obvious effect on low-concentration hexavalent chromium wastewater, and has certain application prospect and value in the aspect of microbial remediation of water bodies polluted by low-concentration hexavalent chromium.
Detailed Description
To further clarify the objects, features and advantages of the present invention, a more particular description of the invention, briefly summarized above, may be had by reference to the embodiment illustrated in the appended drawings, which is now described at .
Curvularia lunata is Curvularia lunata HT02, and the strain is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC NO. 17597.
The invention separates and screens curvularia lunata, re-screens strains and identifies strains
(1) Separation and screening:
collecting a sample: collecting a water sample from a wastewater storage pool of a certain steel plant in Maanshan;
domestication and culture: taking 10mL of water sample, inoculating to sterilized liquid Cnahs medium (containing 10mg/L Cr)6+) Performing shake culture in an incubator at 28 ℃ at the rotation speed of 180 rpm; after 2d of culture, taking 10mL of culture solution for acclimatization culture, and keeping the culture medium and culture conditions unchanged;
coating and screening: continuously acclimatizing and culturing for 3 times, diluting the acclimatized bacterial suspension with a dilution gradient of 10-2、10-3、10-4、10-5、10-6Coating 100. mu.L of diluted bacterial suspension with solid culture medium (containing 10mg/L Cr)6+) (ii) a Transferring the coated flat plate into a constant-temperature incubator at 28 ℃, and standing for culture.
Streaking, when colonies grow on the plate, selecting colonies with appearance, shape and front and back color not , taking edge hypha to transfer into new solid Chaochi culture medium plate (containing 10mg/L Cr6+) Transferring the mixture into a constant-temperature incubator at 28 ℃, and standing and culturing; the above steps are repeated until each new colony on the plate is a pure strain.
6 pure colonies are obtained through the separation screening experiment; respectively inoculating the obtained strains into a flat plate and an inclined plane of a Chao's medium, standing and culturing at 28 ℃ until hyphae grow fully, and then placing in a refrigerator at 4 ℃ for preservation;
(2) bacterial strain rescreening
The 6 strains obtained by screening and separating are screened and selected again by detecting the reduction effect of the strains on hexavalent chromium, and the specific steps are as follows:
inoculating the strains from the plate into sterilized liquid Czochralski culture medium (50mL/250mL conical flask), and culturing at 28 deg.C under shaking at 180 rpm; after 3 days of culture, 1mL of bacterial suspension is taken according to the inoculation amount of 1% (v/v), and inoculated to the medium containing 10mg/L Cr6+In a 50mL/250mL Erlenmeyer flask, and cultured at a rotation speed of 180rpm in an incubator at 28 ℃ with shaking. After 3d, sampling, centrifuging and removing Cr in the water sample6+The concentration was measured and the removal rate was calculated. Finally, the bacterial strain HT02 is determined to have obvious hexavalent chromium removal effect, and the removal rate reaches 43%.
(3) Strain identification
By adopting a molecular biology PCR technology and universal primers ITS1 and ITS4, an ITS1+5.8S + ITS2 sequence of rDNA of the strain is amplified, and the strain is compared with an NCBI GenBank database, has the homology of 99 percent and is identified as curvularia lunata HT 02.
(4) Strain preservation
The inventor names the screened strain with hexavalent chromium reduction effect as Curvularia pinnatifida with the self-coding number of HT02, and sends the strain to the China general microbiological culture Collection center for registration and preservation, wherein the preservation number is CGMCC NO. 17597.
Example 1 reduction experiment of different addition amounts of microbial inoculum on low-concentration hexavalent chromium
(1) Preparation of microbial inoculum containing Curvularia alutata
A part of an agar block with hyphae was picked from a plate or a slant of Curvularia lunata HT02, inoculated into a sterilized liquid medium (50mL/250mL Erlenmeyer flask), and cultured with shaking in an incubator at 28 ℃ at a rotation speed of 180 rpm. Culturing for 3d to obtain seed liquid;
then inoculating the inoculum solution to a sterilized liquid Cnahs medium (100mL/500mL conical flask) according to the inoculation amount of 10% (v/v), and culturing for 5d to prepare a microbial inoculum; after 5 days, the bacterial strain grows well, and mycelium spheres grow in the conical flask, namely the required Curvularia lunata-containing HT02 microbial inoculum.
(2) Preparation of hexavalent chromium wastewater
Preparing a Chao's culture medium solution, and adding a potassium dichromate mother solution to obtain Cr in the culture medium6+The concentration of the chromium is 25mg/L, namely the prepared low-concentration hexavalent chromium wastewater.
(3) Reduction treatment
Adding the prepared microbial inoculum into the prepared hexavalent chromium wastewater according to the addition amount of 0.2%, 1% and 2% (v/v), and carrying out shaking culture at 180rpm at 28 ℃. And sampling every 3d, centrifuging, measuring the concentration of hexavalent chromium in the supernatant, and calculating the removal rate of the hexavalent chromium. The results are given in the following table:
the experimental effect shows that the curvularia lunata microbial inoculum provided by the invention has strong reducing capability on low-concentration hexavalent chromium, and the reducing capability on the hexavalent chromium is gradually increased along with the increase of the addition amount of the microbial inoculum. The treatment lasts for 3d, and the removal rate of hexavalent chromium in the wastewater is about 50%; in the 6d, the concentration of the residual hexavalent chromium in the wastewater is lower than 1 mg/L; at the 9d, under the addition of 1 percent and 2 percent (v/v), the hexavalent chromium is completely reduced, the removal rate can reach 100 percent, and under the addition of 0.2 percent (v/v), the residual hexavalent chromium is only 0.05mg/L and is lower than the limit standard (0.1mg/L) of the hexavalent chromium in the surface V-type water.
Example 2 reduction experiment of different addition amounts of microbial inoculum on high-concentration hexavalent chromium
(1) Preparation of microbial inoculum containing Curvularia HT02
Selecting partial agar block with hypha from flat plate or inclined plane of Curvularia lunata HT02, inoculating into sterilized liquid Chaochou culture medium (50mL/250mL conical flask), culturing at 28 deg.C under shaking at 180rpm for 3d to obtain seed solution;
then inoculating the inoculum solution to a sterilized liquid Cnahs medium (100mL/500mL conical flask) according to the inoculation amount of 10% (v/v), and culturing for 5d to prepare a microbial inoculum; after 5 days, the bacterial strain grows well, and mycelium spheres grow in the conical flask, namely the required Curvularia lunata-containing HT02 microbial inoculum.
(2) Preparation of hexavalent chromium wastewater
Preparing a Chao's culture medium solution, and adding a potassium dichromate mother solution to obtain Cr in the culture medium6+The concentration of the hexavalent chromium is 500mg/L, namely the prepared high-concentration hexavalent chromium wastewater.
(3) Reduction treatment
Adding the prepared microbial inoculum into the prepared hexavalent chromium wastewater according to the addition amount of 0.2%, 1% and 2% (v/v), and carrying out shaking culture at 180rpm at 28 ℃. And sampling every 3d, centrifuging, measuring the concentration of hexavalent chromium in the supernatant, and calculating the removal rate of the hexavalent chromium. The results are given in the following table:
according to the experimental results, the curvularia lunata reduction treatment effect of the curvularia lunata on the high-concentration hexavalent chromium wastewater (500mg/L) is , although the removal rate of hexavalent chromium is increased with the increase of the addition amount of the microbial inoculum, after 9d treatment, the removal rates of hexavalent chromium of two groups of 1% and 2% (v/v) reach about 30%, and compared with the removal rate of 99.9% of the low-concentration hexavalent chromium wastewater, the curvularia lunata reduction treatment effect has a treatment effect difference of .
Therefore, the curvularia lunata newly screened by the invention has -determined reduction treatment effect on hexavalent chromium in wastewater through reduction treatment on low-concentration and high-concentration hexavalent chromium wastewater, particularly has better and obvious effect on low-concentration hexavalent chromium wastewater, and has -determined application prospect and value in the aspect of microbial remediation of water bodies polluted by low-concentration hexavalent chromium.
The above embodiments are illustrative of the above disclosure of the present invention at step , but it should not be understood that the scope of the above subject matter is limited to the above embodiments.
Claims (7)
1, Curvularia lunata, the Latin name of the strain is Curvularia lunata HT02, the preservation unit is China general microbiological culture Collection center, the preservation date is 2019, 4 and 15 days, and the preservation number is CGMCC NO. 17597.
The microbial inoculum of , wherein the microbial inoculum comprises Curvularia lunata as described in claim 1.
3. Use of the bacterial agent of claim 2 in the preparation of a product for treating hexavalent chromium containing wastewater.
4. Use of the bacterial agent according to claim 2 for the treatment of wastewater containing hexavalent chromium.
5. The use of the bacterial formulation of claim 2 in the preparation of a product for treating waste water containing low concentrations of hexavalent chromium.
6. Use of the microbial inoculum according to claim 2 for the treatment of wastewater containing low concentrations of hexavalent chromium.
A method for treating hexavalent chromium-containing waste water by using the Curvularia lunata or its bacterial suspension according to claim 1, in .
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106085900A (en) * | 2016-06-07 | 2016-11-09 | 中国科学院生态环境研究中心 | Fiber germ and the application that chromate waste water is processed thereof |
| WO2017122227A1 (en) * | 2016-01-13 | 2017-07-20 | Shri Amm Murugappa Chettiar Research Centre (Mcrc) | Mrsa and vrsa resistant textile materials |
| CN108555007A (en) * | 2018-03-28 | 2018-09-21 | 中冶华天工程技术有限公司 | The reduction of soil-repairing agent and hexavalent chromium polluted soil stabilizes restorative procedure |
| CN109182178A (en) * | 2018-09-12 | 2019-01-11 | 山西省农业科学院果树研究所 | One plant of bacterial strain and its application in reparation mild or moderate chromium-polluted soil in situ with chromium tolerance and Cr (VI) removal ability |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017122227A1 (en) * | 2016-01-13 | 2017-07-20 | Shri Amm Murugappa Chettiar Research Centre (Mcrc) | Mrsa and vrsa resistant textile materials |
| CN106085900A (en) * | 2016-06-07 | 2016-11-09 | 中国科学院生态环境研究中心 | Fiber germ and the application that chromate waste water is processed thereof |
| CN108555007A (en) * | 2018-03-28 | 2018-09-21 | 中冶华天工程技术有限公司 | The reduction of soil-repairing agent and hexavalent chromium polluted soil stabilizes restorative procedure |
| CN109182178A (en) * | 2018-09-12 | 2019-01-11 | 山西省农业科学院果树研究所 | One plant of bacterial strain and its application in reparation mild or moderate chromium-polluted soil in situ with chromium tolerance and Cr (VI) removal ability |
Non-Patent Citations (3)
| Title |
|---|
| RITA DE CÁSSIA M. DE MIRANDA ET AL.: "Biotreatment of textile effluent in static bioreactor by Curvularia lunata URM 6179 and Phanerochaete chrysosporium URM 6181", 《BIORESOURCE TECHNOLOGY》 * |
| 周芳如等: "高效去除镉污染真菌的筛选与鉴定", 《浙江农业学报》 * |
| 湛方栋等: "丝状真菌耐受重金属的细胞机制研究", 《云南农业大学学报》 * |
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