CN110726654B

CN110726654B - Microbial sensor

Info

Publication number: CN110726654B
Application number: CN201911019937.2A
Authority: CN
Inventors: 费逸; 罗志康; 姜超
Original assignee: Anhui Kangyuan Biotechnology Co ltd
Current assignee: Anhui Kangyuan Biotechnology Co.,Ltd.
Priority date: 2019-10-24
Filing date: 2019-10-24
Publication date: 2022-03-22
Anticipated expiration: 2039-10-24
Also published as: CN110726654A

Abstract

The invention belongs to the technical field of sensors, and particularly relates to a microbial sensor which comprises a substrate, a flushing mechanism, a liquid storage mechanism, a limiting mechanism and a dirt collecting mechanism, wherein the substrate made of ceramics is provided with a detection mechanism used for detecting microbes, the detection mechanism comprises a conducting ring, a conducting rod and a detector, the substrate is provided with two symmetrically distributed grooves, the conducting ring is fixedly embedded in the grooves, the conducting ring is electrically connected with the detector fixedly arranged at the rear end of the substrate through the conducting rod, the liquid storage mechanism is arranged in an installation cavity on the flushing mechanism, the flushing mechanism is arranged on the substrate, the end surface of the substrate close to a liquid drainage spray pipe is provided with two symmetrically distributed clamping grooves, and two vertically arranged liquid injection cylinders are fixedly arranged on the substrate. The microbial sensor provided by the invention has the advantages of cleaning and dirty liquid treatment functions.

Description

Microbial sensor

Technical Field

The invention belongs to the technical field of sensors, and particularly relates to a microbial sensor.

Background

In recent years, food poisoning events caused by pathogenic microorganisms are frequently reported. According to the report of the world health organization, the diarrhea caused by the food-borne pathogenic bacteria pollution is billions of cases every year around the world, and about 170 ten thousand cases of children under 15 years of age die. In a key control point system of food hazard analysis, microorganism detection plays an important role, so that the detection of food-borne pathogenic microorganisms is a key link for preventing and controlling food-borne diseases. Along with the diversification of raw material sources of the current food processing industry, the turnover and running of products are fast, and the urgent requirements of accelerating the detection speed, enhancing the detection specificity and improving the detection sensitivity of food microorganism detection are met.

When detecting escherichia coli in a liquid food processing factory, sampling detection needs to be carried out on various sampling points, the heads of the sensors need to be washed when the microbial sensors are used in a large amount of liquid food, the microbial sensors need to be collected for multiple times in the stirring process when the food needing fermentation is detected, and part of the microbial sensors are difficult to clean when the microbial liquid is detected and the sewage is difficult to treat after the detection. Therefore, it is necessary to provide a microbial sensor to solve the above technical problems.

Disclosure of Invention

In order to solve the technical problems, the invention provides a microbial sensor with functions of cleaning and treating foul solution.

The invention provides a microbial sensor which comprises a substrate, a flushing mechanism, a liquid storage mechanism, a limiting mechanism and a sewage collecting mechanism, wherein the substrate made of ceramics is provided with a detection mechanism for detecting microbes; the washing mechanism is arranged on a substrate and comprises an installation cavity, liquid guide pipes, a first electric control valve, a second electric control valve and a liquid drainage spray pipe, wherein the installation cavity is formed in the middle of the substrate and is respectively communicated with two conducting rings through the two liquid guide pipes; the flushing mechanism further comprises an impeller, a rotating shaft, a torsional spring and a filter screen, the liquid discharge spray pipe is connected with the impeller, the impeller is installed on the rotating shaft, the filter screen is fixed on the rotating shaft, and the torsional spring is installed on the rotating shaft; the liquid storage mechanism is arranged in an installation cavity on the flushing mechanism; the end face, close to the liquid discharge spray pipe, of the base is provided with two clamping grooves which are symmetrically distributed, and limiting mechanisms are arranged in the two clamping grooves; the dirt collecting mechanism is fixedly arranged on the substrate through a limiting mechanism.

Specifically, stock solution mechanism includes the liquid storage ball, annotates liquid pipe and check valve, annotate that the liquid pipe sets up perpendicularly and fixed mounting on the installation cavity, and the top fixed mounting who annotates the liquid pipe has the liquid storage ball, fixed mounting has the check valve on the notes liquid pipe, the inlet has been seted up on the liquid storage ball.

Specifically, stop gear includes joint chamber, slide bar, pull ring, lagging and spring, the joint chamber is seted up in the basement in the joint groove outside, and the joint chamber communicates with the joint groove each other, the slide bar insert establish in the joint intracavity and with joint chamber sliding connection, and the other end of slide bar runs through lateral wall and the fixed mounting of basement and have the pull ring, fixed cover has connect the lagging on the slide bar of joint intracavity, and lagging and joint chamber sliding connection, the cluster has the spring on the slide bar.

The sewage collecting mechanism comprises a sewage collecting box, a liquid inlet, clamping plates and limiting grooves, wherein the liquid inlet is formed in the inner wall of the sewage collecting box, the two symmetrical liquid inlets are formed in the inner wall of the sewage collecting box, the liquid inlet is matched with the liquid discharge spray pipe, the clamping plates are fixedly mounted on the two sides of the inner wall of the sewage collecting box, and the limiting grooves are formed in the side walls, back to back, of the two clamping plates.

Specifically, the lateral wall fixed connection in joint groove is kept away from to the one end of spring and lagging, and the other end of spring and the inner wall fixed connection in joint chamber stop gear.

Specifically, one end of the sliding rod, which is close to the clamping groove, is provided with a transition cambered surface.

Specifically, two perpendicularly arranged liquid injection cylinders are fixedly mounted on the substrate, and the two liquid injection cylinders are respectively communicated with the two conducting rings on the detection mechanism.

Compared with the related art, the microbial sensor provided by the invention has the following beneficial effects:

(1) according to the invention, the conducting ring is flushed by clean water in the liquid storage ball through the flushing mechanism by opening the one-way valve, so that the device can be connected for microorganism detection.

(2) In the process of flushing the conducting ring, the conducting ring is cleaned under the matching action of the flushing mechanism and the dirt collecting mechanism, the liquid discharge spray pipe drives the impeller to rotate, the impeller is arranged on the rotating shaft, the rotating shaft is driven to rotate through the liquid discharge spray pipe, accumulated floccules on the filter screen on the rotating shaft are shaken off, and meanwhile, the rotating shaft returns to the original position under the reset action of the torsion spring.

Drawings

FIG. 1 is a schematic structural view of a preferred embodiment of a microbial sensor according to the present invention;

FIG. 2 is a schematic structural view of the detection mechanism and the flushing mechanism shown in FIG. 1;

FIG. 3 is a schematic structural view of the limiting mechanism shown in FIG. 1;

FIG. 4 is a schematic structural view of the dirt collecting mechanism shown in FIG. 1;

fig. 5 is a schematic view of the internal structure of the dirt collecting box shown in fig. 4.

Reference numbers in the figures: 1. the device comprises a substrate, 2, a detection mechanism, 21, a conducting ring, 22, a conducting rod, 23, a detector, 3, a liquid injection cylinder, 4, a flushing mechanism, 41, an installation cavity, 42, a liquid guide pipe, 43, a first electric control valve, 44, a second electric control valve, 45, a liquid discharge spray pipe, 46, an impeller, 47, a rotating shaft, 48, a torsion spring, 49, a filter screen, 5, a liquid storage mechanism, 51, a liquid storage ball, 52, a liquid injection pipe, 53, a one-way valve, 6, a clamping groove, 7, a limiting mechanism, 71, a clamping cavity, 72, a sliding rod, 73, a pull ring, 74, a sleeve plate, 75, a spring, 8, a dirt collection mechanism, 81, a dirt collection box, 82, a liquid inlet, 83, a clamping plate, 84 and a limiting groove.

Detailed Description

The invention is further described with reference to the following figures and embodiments.

Referring to fig. 1-5, a microbial sensor includes a substrate 1, a washing mechanism 4, a liquid storage mechanism 5, a limiting mechanism 7, and a dirt collecting mechanism 8, wherein the substrate 1 made of ceramic is provided with a detection mechanism 2 for detecting microbes, the detection mechanism 2 includes a conductive ring 21, a conductive rod 22, and a detector 23, two grooves are formed in the substrate 1, the conductive ring 21 is fixed and embedded in the grooves, and the conductive ring 21 is electrically connected with the detector 23 fixed at the rear end of the substrate 1 through the conductive rod 22.

It should be noted that: the microbes to be detected are respectively dripped on the two conductive rings 21, the identification molecules fixed on the surfaces of the conductive rings 21 are combined with a target object to generate impedance signals, the impedance change is related to the concentration of the target object, the change of electrical signals such as resistance, capacitance, inductance and the like is measured by the conductive rod 22 through the detector 23, and then the data is processed, so that the concentration of the target object can be quickly and accurately detected.

Referring to fig. 1, the liquid storage mechanism 5 is installed in the installation cavity 41 of the flushing mechanism 4, the liquid storage mechanism 5 includes a liquid storage ball 51, a liquid injection pipe 52 and a one-way valve 53, the liquid injection pipe 52 is vertically arranged and fixedly installed on the installation cavity 41, the liquid storage ball 51 is fixedly installed at the top of the liquid injection pipe 52, the one-way valve 53 is fixedly installed on the liquid injection pipe 52, and a liquid inlet is opened on the liquid storage ball 51; sufficient clear water is stored in the liquid storage ball 51, and when the conducting ring 21 needs to be cleaned, the one-way valve 53 is opened, so that the clear water in the liquid storage ball 51 washes the conducting ring 21 through the washing mechanism 4, and therefore the equipment can be connected for microorganism detection.

Referring to fig. 2, washing mechanism 4 installs on basement 1, washing mechanism 4 includes installation cavity 41, catheter 42, first electric control valve 43, second electric control valve 44 and flowing back spray tube 45, installation cavity 41 is seted up at basement 1 middle part, and installation cavity 41 communicates each other, two with two conducting rings 21 respectively through two catheters 42 equal fixed mounting has first electric control valve 43 on the catheter 42, two equal fixed mounting has flowing back spray tube 45 on the conducting ring 21, and fixed mounting has second electric control valve 44 on the flowing back spray tube 45, just washing mechanism 4 still includes impeller 46, pivot 47, torsional spring 48 and filter screen 49, flowing back spray tube 45 is connected with impeller 46, just impeller 46 installs in pivot 47, just be fixed with filter screen 49 in the pivot 47, just install torsional spring 48 in the pivot 47.

It should be noted that: the first electric control valve 43 and the second electric control valve 44 are opened, so that the clean water stored in the liquid storage ball 51 enters the conductive ring 21 through the liquid guide tube 42 and washes out the residual microorganism liquid in the conductive ring 21 to be discharged to the sewage collection box 81 through the liquid discharge nozzle 45.

Referring to fig. 3, two symmetrically distributed clamping grooves 6 are formed in the end surface of the substrate 1 close to the liquid discharge nozzle 45, and two clamping grooves 6 are internally provided with a limiting mechanism 7, the limiting mechanism 7 comprises a clamping cavity 71, a sliding rod 72, a pull ring 73, a sleeve plate 74 and a spring 75, the clamping cavity 71 is formed in the substrate 1 outside the clamping groove 6, the clamping cavity 71 is communicated with the clamping grooves 6, the sliding rod 72 is inserted in the clamping cavity 71 and is in sliding connection with the clamping cavity 71, the other end of the sliding rod 72 penetrates through the side wall of the substrate 1 and is fixedly provided with the pull ring 73, one end of the sliding rod 72 close to the clamping groove 6 is provided with an excessive arc surface, the sliding rod 72 in the clamping cavity 71 is fixedly sleeved with the sleeve plate 74, the sleeve plate 74 is in sliding connection with the clamping cavity 71, the sliding rod 72 is stringed with the spring 75, one end of the spring 75 is fixedly connected with the side wall of the sleeve plate 74 far away from the clamping groove 6, the other end of the spring 75 is fixedly connected with the inner wall of the clamping cavity 71 and is connected with the limiting mechanism 7; dirty box 81 of collection in the dirty mechanism 8 of dismantlement basement 1 that can be nimble through stop gear 7 to be convenient for clean dirty box 81 of collection.

Refer to fig. 1 and 4 and show, dirty mechanism 8 of collection passes through stop gear 7 fixed mounting on basement 1, dirty mechanism 8 of collection is including the dirty box 81 of collection, inlet 82, joint board 83 and spacing groove 84, the inlet 82 that two symmetries set up is seted up to the inner wall of the dirty box 81 of collection, and inlet 82 matches each other with flowing back spray tube 45, the equal fixed mounting in both sides of the dirty box 81 inner wall of collection has joint board 83, and two joint board 83 lateral walls that carry on the back mutually have all been seted up spacing groove 84, and the dirty box 81 of collection in the dirty mechanism 8 of collection can be to collecting the microorganism liquid and the flush fluid after the detection, has avoided equipment to be difficult to collect the shortcoming of handling microorganism liquid and flush fluid.

Referring to fig. 1, two vertically arranged liquid injection cylinders 3 are fixedly mounted on the substrate 1, and the two liquid injection cylinders 3 are respectively communicated with two conductive rings 21 on the detection mechanism 2, so as to drip microorganism liquid into the conductive rings 21.

The working principle of the microbial sensor provided by the invention is as follows: before detecting the microbial liquid, inserting two clamping plates 83 on the sewage collection box 81 into two clamping grooves 6 on the substrate 1, compressing the spring 75 through the sleeve plate 74 due to the extrusion action of the clamping plates 83, so that one end of the sliding rod 72, which is provided with a transition arc surface, is clamped into a limiting groove 84 on the outer wall of the clamping plate 83, and simultaneously inserting two liquid drainage spray pipes 45 on the flushing mechanism 4 into the sewage collection box 81 through liquid inlets 82 on the sewage collection box 81 respectively, thereby completing the installation of the sewage collection box 81; when in detection, microorganism liquid is dripped onto the conducting ring 21 through the liquid injection cylinder 3, identification molecules fixed on the surface of the conducting ring 21 are combined with a target object and generate an impedance signal, the impedance change is related to the concentration of the target object, the conducting rod 22 measures the change of electrical signals such as resistance, capacitance, inductance and the like through the detector 23, and then data are processed, so that the concentration of the target object can be quickly and accurately detected, after the detection of the microorganism liquid is completed, the check valve 53, the first electric control valve 43 and the second electric control valve 44 are opened, so that clear water in the liquid storage ball 51 washes the conducting ring 21 through the washing mechanism 4, the clear water stored in the liquid storage ball 51 enters the conducting ring 21 through the liquid guide pipe 42 and washes out the microorganism liquid remained in the conducting ring 21 to be discharged into the sewage collection box 81 through the liquid discharge pipe 45, and the sewage collection box 81 can collect the detected microorganism liquid and washing liquid, the shortcoming that the device is difficult to collect and process microorganism liquid and flushing liquid is avoided, and when dirty liquid needs to be processed, the pulling ring 73 is pulled to enable the sliding rod 72 clamped into the limiting groove 84 to slide out of the limiting groove 84, and the dirt collecting box 81 slides outwards to be capable of unloading the dirt collecting box 81 on the substrate 1 to process the dirty liquid in the dirt collecting box 81.

The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. The microbial sensor is characterized by comprising a substrate (1), a flushing mechanism (4), a liquid storage mechanism (5), a limiting mechanism (7) and a dirt collecting mechanism (8), wherein the substrate (1) is made of ceramic, the detecting mechanism (2) for detecting microbes is arranged on the substrate (1), the detecting mechanism (2) comprises a conducting ring (21), a conducting rod (22) and a detector (23), two symmetrically distributed grooves are formed in the substrate (1), the conducting ring (21) is fixedly embedded in the grooves, and the conducting ring (21) is electrically connected with the detector (23) fixedly arranged at the rear end of the substrate (1) through the conducting rod (22); the washing mechanism (4) is installed on the substrate (1), the washing mechanism (4) comprises an installation cavity (41), liquid guide pipes (42), a first electric control valve (43), a second electric control valve (44) and liquid drainage spray pipes (45), the installation cavity (41) is arranged in the middle of the substrate (1), the installation cavity (41) is communicated with the two conducting rings (21) through the two liquid guide pipes (42), the first electric control valve (43) is fixedly installed on the two liquid guide pipes (42), the liquid drainage spray pipes (45) are fixedly installed on the two conducting rings (21), and the second electric control valve (44) is fixedly installed on the liquid drainage spray pipes (45); the flushing mechanism (4) further comprises an impeller (46), a rotating shaft (47), a torsion spring (48) and a filter screen (49), the liquid discharge spray pipe (45) is connected with the impeller (46), the impeller (46) is installed on the rotating shaft (47), the filter screen (49) is fixed on the rotating shaft (47), and the torsion spring (48) is installed on the rotating shaft (47); the liquid storage mechanism (5) is arranged in an installation cavity (41) on the flushing mechanism (4); two clamping grooves (6) which are symmetrically distributed are formed in the end face, close to the liquid discharge spray pipe (45), of the substrate (1), and limiting mechanisms (7) are mounted in the two clamping grooves (6); the sewage collecting mechanism (8) is fixedly arranged on the substrate (1) through a limiting mechanism (7);

the liquid storage mechanism (5) comprises a liquid storage ball (51), a liquid injection pipe (52) and a one-way valve (53), the liquid injection pipe (52) is vertically arranged and fixedly installed on the installation cavity (41), the top of the liquid injection pipe (52) is fixedly provided with the liquid storage ball (51), the liquid injection pipe (52) is fixedly provided with the one-way valve (53), and the liquid storage ball (51) is provided with a liquid inlet;

dirty mechanism (8) of collection is including dirty box (81), inlet (82), joint board (83) and spacing groove (84) of collection, inlet (82) that two symmetries set up are seted up to the inner wall of the dirty box (81) of collection, and inlet (82) and flowing back spray tube (45) match each other, the equal fixed mounting in both sides of the dirty box (81) inner wall of collection has joint board (83), and two joint board (83) lateral walls that carry on the back mutually have all seted up spacing groove (84).

2. The microbial sensor according to claim 1, wherein the limiting mechanism (7) comprises a clamping cavity (71), a sliding rod (72), a pull ring (73), a sleeve plate (74) and a spring (75), the clamping cavity (71) is formed in the substrate (1) outside the clamping groove (6), the clamping cavity (71) is communicated with the clamping groove (6), the sliding rod (72) is inserted in the clamping cavity (71) and is in sliding connection with the clamping cavity (71), the other end of the sliding rod (72) penetrates through the side wall of the substrate (1) and is fixedly provided with the pull ring (73), the sleeve plate (74) is fixedly sleeved on the sliding rod (72) in the clamping cavity (71), the sleeve plate (74) is in sliding connection with the clamping cavity (71), and the spring (75) is connected on the sliding rod (72) in series.

3. The microbial sensor according to claim 2, wherein one end of the spring (75) is fixedly connected with the side wall of the sleeve plate (74) far away from the clamping groove (6), and the other end of the spring (75) is fixedly connected with the limiting mechanism (7) on the inner wall of the clamping cavity (71).

4. The microorganism sensor according to claim 2, wherein the end of the sliding rod (72) near the clamping groove (6) is provided with an over-arc.

5. A biosensor as claimed in claim 1, wherein two vertically arranged liquid injection cylinders (3) are fixedly mounted on the substrate (1), and the two liquid injection cylinders (3) are respectively communicated with the two conductive rings (21) of the detection mechanism (2).