CN110721093A - PLGA nano-particle deciduous tooth root canal filling material and preparation method thereof - Google Patents
PLGA nano-particle deciduous tooth root canal filling material and preparation method thereof Download PDFInfo
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Abstract
A PLGA nano-particle deciduous tooth root canal filling material and a preparation method thereof belong to the field of medical materials. The PLGA nano-particle deciduous tooth root canal filling material comprises, by mass, from 50% to 99% of PLGA, from 1% to 50% of a drug, PLGA being a main body scaffold material, and the drug being an adjusting material for adjusting the degradation rate of the material and increasing the therapeutic effect. Adding PLGA into an acetone solution, stirring the solution to obtain a solution A, pouring the solution A into a polyvinyl alcohol solution, stirring, standing until acetone is volatilized, centrifuging, removing supernate, cleaning the precipitate with deionized water, centrifuging to obtain the precipitate again, carrying out ultrasonic mixing treatment, centrifuging again, obtaining the precipitate, and air-drying for later use to obtain the PLGA nano-particle deciduous tooth root canal filling material. Convenient use, degradability and high safety, and can improve the curative effect of the operation.
Description
Technical Field
The invention belongs to the field of medical materials, and particularly relates to an absorbable drug-loaded PLGA nanoparticle deciduous tooth root canal filling material and a preparation method thereof.
Background
Since the first application of PGA (polyglycolic acid) to clinical orthopedics in Rokkane in 1984, experiments and clinical researches on biodegradable absorbable materials have been rapidly developed, and certain results have been achieved. Such materials are currently rapidly developing in trauma orthopedics.
Deciduous teeth, which are a special body component, are gradually absorbed by the tooth root with age and eventually fall off, so that the root canal filling material of the deciduous teeth must be absorbable. The deciduous tooth root tip is absorbed, and the material in the root canal is in direct contact with the bone tissue, so the material must be a biosafety material. The root canal treatment of deciduous teeth is difficult to fill tightly, and therefore, it is preferable that the filled drug sustainably release a bacteriostatic component or a drug containing a curable component. The deciduous tooth root canal therapy is difficult to fill tightly, and the nano particles can well simulate the human tissue structure, thereby showing obvious advantages. Combining the above characteristics, PLGA nanoparticles are a suitable choice.
Disclosure of Invention
The invention aims to provide an absorbable drug-loaded PLGA nanoparticle deciduous tooth root canal filling material and a preparation method thereof.
The PLGA nanoparticle deciduous tooth root canal filling material comprises, by mass, PLGA 50-99% and a carried drug 1-50%, wherein the PLGA is a main stent material, and the carried drug is an adjusting material for adjusting the degradation rate of the material and increasing the treatment effect.
The structure formed by the PLGA and the carried medicine can be that the medicine is wrapped in the PLGA nano-particles or mixed with the PLGA, and the PLGA nano-particles exist independently.
The carried medicine can be powdered medicine, water-soluble medicine, fat-soluble medicine, acid-base liquid dissolved medicine and the like; the carried medicine can be selected from one of ZnO, growth factor, other antibacterial and anti-inflammatory medicines, and the like, and the growth factor can be selected from one of fibroblast growth factor, connective tissue growth factor, platelet-derived growth factor-beta, and the like; the other antibacterial and anti-inflammatory medicines can be nano-scale granular medicines, the nano-scale granular medicines can be one of nano-silver, soluble medicines, Chinese herbal medicines, Chinese patent medicines and the like, and the soluble medicines can be medicines with the effects of promoting tissue healing, inhibiting bacteria and relieving pain and the like, such as vitamin C, lidocaine hydrochloride and the like; the Chinese herbal medicine can be selected from herba Epimedii, radix Zanthoxyli, etc. with effects of promoting blood circulation, inhibiting bacteria and promoting tissue growth; the Chinese patent medicine can be one selected from tetracycline, amoxicillin, metronidazole, emodin, puerarin, hedysarum polybotrys polysaccharide, ferritin and the like.
The method for mixing the loaded drug and the PLGA nanoparticles can be as follows: mixing the loaded medicine with the PLGA solution to form nano particles; or the loaded medicine and the PLGA nano-particles are directly physically mixed; or PLGA soaks the medicine solution, dry to form; the mass fraction of the PLGA solution can be 2-40%, and the mass percentage of the drug solution can be 1-50%.
The PLGA nano-particle deciduous tooth root canal filling material can adopt but not limited to an emulsion solvent volatilization method, a double-emulsion solvent diffusion method, a spray drying method, a double-emulsion solvent volatilization method and the like.
The preparation method of the PLGA nano-particle deciduous tooth root canal filling material comprises the following steps:
1) adding PLGA into an acetone solution, and stirring the solution at 750-1000 r/min to obtain a solution A;
in the step 1), the ratio of the acetone solution to the PLGA may be (3-5 mL): 25-100 mg), wherein the acetone solution is calculated by volume and the PLGA is calculated by mass.
2) Pouring the solution A obtained in the step 1) into 20-80 ml of 1.5% -5% (v/v) polyvinyl alcohol solution, stirring, standing until acetone is volatilized, centrifuging, removing supernate, washing the precipitate with deionized water, centrifuging again, taking the precipitate, ultrasonically mixing uniformly, centrifuging again, taking the precipitate, and air-drying for later use to obtain the PLGA nano-particle deciduous tooth root canal filling material.
In the step 2), the addition amount of the polyvinyl alcohol solution can be 20-80 ml, and the concentration of the polyvinyl alcohol solution can be 1.5-5% (v/v); the stirring time can be 6-10 h, and the mixture can be kept stand until acetone is volatilized and then centrifuged at 12000-20000 rpm for 12-20 min; the time of the ultrasonic uniform mixing treatment can be 15-40 s.
The invention is provided on the basis of fully considering the characteristics of pathogenic mechanism, treatment, prognosis and the like of deciduous tooth root canal filling, and the structure and the components of the material are beneficial to the recovery and the functional recovery of the dental pulp apicitis and the reduction of the recurrence rate.
The invention has the following outstanding technical effects:
1) the PLGA nano-particle deciduous tooth root canal filling material can be absorbed and can not be remained in the body along with the absorption of the deciduous tooth root.
2) The PLGA nano-particle deciduous tooth root canal filling material is nano-particles and can be well filled in deciduous tooth root canals.
3) The PLGA nano-particle deciduous tooth root canal filling material has good biocompatibility, is in contact with bone tissues in the apical area, and does not produce side effects.
4) The PLGA nano-particle deciduous tooth root canal filling material can be combined with a medicament, can be a bacteriostatic medicament, can also be a therapeutic medicament, and can promote the recovery of inflammation.
5) The PLGA nano-particle deciduous tooth root canal filling material is a novel medical drug-carrying biodegradable material, is convenient to use, degradable and high in safety, and can improve the operation curative effect.
Detailed Description
The following examples further illustrate the invention.
Example 1
PLGA50mg was added to 5mL of acetone solution. The solution is placed on a magnetic stirrer and is completely dissolved. The solution was stirred at 800 r/min. The solution was slowly poured into 40ml of 2% (v/v) polyvinyl alcohol solution and magnetic stirring was continued for 8 h. Standing until acetone is volatilized. Centrifuging at 15,000rpm for 15min, removing supernatant, washing the precipitate with deionized water for 3 times, centrifuging, and collecting the precipitate again. Ultrasonically mixing for 20s, centrifuging again, and collecting precipitate. And (5) air-drying for later use. Mixing the powder-dried PLGA and the nano-silver particles to form a PLGA/nano-silver mixture
Example 2
5mL of acetone solution was added with PLGA50mg and erythromycin 40 mg. The solution is placed on a magnetic stirrer and is completely dissolved. The solution was stirred at 800 r/min. The solution was slowly poured into 40ml of 2% (v/v) polyvinyl alcohol solution and magnetic stirring was continued for 8 h. Standing until acetone is volatilized. Centrifuging at 15,000rpm for 15min, removing supernatant, washing the precipitate with deionized water for 3 times, centrifuging, and collecting the precipitate again. Ultrasonically mixing for 20s, centrifuging again, and collecting precipitate. And (5) air-drying for later use. A PLGA/erythromycin mixture was formed.
Example 3
5mL of acetone solution was added with PLGA50mg and 2 g of potassium iodide. The solution is placed on a magnetic stirrer and is completely dissolved. The solution was stirred at 800 r/min. The solution was slowly poured into 40ml of 2% (v/v) polyvinyl alcohol solution and magnetic stirring was continued for 8 h. Standing until acetone is volatilized. Centrifuging at 15,000rpm for 15min, removing supernatant, washing the precipitate with deionized water for 3 times, centrifuging, and collecting the precipitate again. Ultrasonically mixing for 20s, centrifuging again, and collecting precipitate. And (5) air-drying for later use. And mixing the powder-dried PLGA and the nano-silver nanoparticles to form a PLGA/potassium iodide mixture.
Example 4
5mL of acetone solution was added with PLGA50mg and Epimedium 2 g. The solution is placed on a magnetic stirrer and is completely dissolved. The solution was stirred at 800 r/min. The solution was slowly poured into 40ml of 2% (v/v) polyvinyl alcohol solution and magnetic stirring was continued for 8 h. Standing until acetone is volatilized. Centrifuging at 15,000rpm for 15min, removing supernatant, washing the precipitate with deionized water for 3 times, centrifuging, and collecting the precipitate again. Ultrasonically mixing for 20s, centrifuging again, and collecting precipitate. And (5) air-drying for later use. And mixing the powder-dried PLGA and the nano-silver nanoparticles to form a PLGA/potassium iodide mixture.
Example 5
PLGA50mg and metronidazole 2 g were added to 5mL of acetone solution. The solution is placed on a magnetic stirrer and is completely dissolved. The solution was stirred at 800 r/min. The solution was slowly poured into 40ml of 2% (v/v) polyvinyl alcohol solution and magnetic stirring was continued for 8 h. Standing until acetone is volatilized. Centrifuging at 15,000rpm for 15min, removing supernatant, washing the precipitate with deionized water for 3 times, centrifuging, and collecting the precipitate again. Ultrasonically mixing for 20s, centrifuging again, and collecting precipitate. And (5) air-drying for later use. And mixing the powder-dried PLGA and the nano-silver nanoparticles to form a PLGA/potassium iodide mixture.
Example 6
PLGA50mg and 2 grams of lactoferrin were added to 5mL of acetone solution. The solution is placed on a magnetic stirrer and is completely dissolved. The solution was stirred at 800 r/min. The solution was slowly poured into 40ml of 2% (v/v) polyvinyl alcohol solution and magnetic stirring was continued for 8 h. Standing until acetone is volatilized. Centrifuging at 15,000rpm for 15min, removing supernatant, washing the precipitate with deionized water for 3 times, centrifuging, and collecting the precipitate again. Ultrasonically mixing for 20s, centrifuging again, and collecting precipitate. And (5) air-drying for later use. And mixing the powder-dried PLGA and the nano-silver nanoparticles to form a PLGA/potassium iodide mixture.
Example 7
5mL of acetone solution was added with PLGA50mg and 2 g of rambutan. The solution is placed on a magnetic stirrer and is completely dissolved. The solution was stirred at 800 r/min. The solution was slowly poured into 40ml of 2% (v/v) polyvinyl alcohol solution and magnetic stirring was continued for 8 h. Standing until acetone is volatilized. Centrifuging at 15,000rpm for 15min, removing supernatant, washing the precipitate with deionized water for 3 times, centrifuging, and collecting the precipitate again. Ultrasonically mixing for 20s, centrifuging again, and collecting precipitate. And (5) air-drying for later use. And mixing the powder-dried PLGA and the nano-silver nanoparticles to form a PLGA/potassium iodide mixture.
Example 8
PLGA50mg and 2 grams of calcium hydroxide were added to 5mL of acetone solution. The solution is placed on a magnetic stirrer and is completely dissolved. The solution was stirred at 800 r/min. The solution was slowly poured into 40ml of 2% (v/v) polyvinyl alcohol solution and magnetic stirring was continued for 8 h. Standing until acetone is volatilized. Centrifuging at 15,000rpm for 15min, removing supernatant, washing the precipitate with deionized water for 3 times, centrifuging, and collecting the precipitate again. Ultrasonically mixing for 20s, centrifuging again, and collecting precipitate. And (5) air-drying for later use. And mixing the powder-dried PLGA and the nano-silver nanoparticles to form a PLGA/potassium iodide mixture.
Claims (10)
1. The PLGA nanoparticle deciduous tooth root canal filling material is characterized by comprising, by mass, from 50% to 99% of PLGA and from 1% to 50% of a drug, wherein the PLGA is a main stent material, and the drug is a regulating material for regulating the degradation rate of the material and increasing the treatment effect.
2. The PLGA nanoparticle deciduous tooth root canal filling material according to claim 1, wherein the PLGA and the loaded drug form a structure in which the drug is encapsulated in the PLGA nanoparticle or mixed with PLGA, and the PLGA nanoparticle are independently present.
3. The PLGA nanoparticle deciduous tooth root canal filling material according to claim 1, wherein the drug to be loaded is a powdered drug, a water-soluble drug, a fat-soluble drug, or an acid-base liquid-soluble drug.
4. The PLGA nanoparticle deciduous tooth root canal filling material according to claim 1, wherein the loaded drug is selected from one of ZnO, growth factor, and other antibacterial and anti-inflammatory drugs.
5. The PLGA nanoparticle deciduous tooth root canal filling material according to claim 4, wherein the growth factor is selected from the group consisting of fibroblast growth factor, connective tissue growth factor, platelet-derived growth factor- β; the other antibacterial and anti-inflammatory medicines are nano-scale granular medicines, the nano-scale granular medicines can be selected from one of nano-silver, soluble medicines, Chinese herbal medicines and Chinese patent medicines, and the soluble medicines can be selected from vitamin C and lidocaine hydrochloride, so that the antibacterial and analgesic medicines for promoting tissue healing are obtained; the Chinese herbal medicine can be selected from herba Epimedii, radix Zanthoxyli, etc. with effects of promoting blood circulation, inhibiting bacteria and promoting tissue growth; the Chinese patent medicine can be one selected from tetracycline, amoxicillin, metronidazole, emodin, puerarin, hedysarum polybotrys polysaccharide and ferritin.
6. The PLGA nanoparticle deciduous tooth root canal filling material according to claim 1, wherein the loaded drug and the PLGA nanoparticles are mixed by a method comprising: mixing the loaded medicine with the PLGA solution to form nano particles; or the loaded medicine and the PLGA nano-particles are directly physically mixed; or PLGA soaks the medicine solution, dry to form; the mass fraction of the PLGA solution can be 2-40%, and the mass percentage of the drug solution can be 1-50%.
7. The PLGA nanoparticle deciduous tooth root canal filling material according to claim 1, wherein the PLGA nanoparticle deciduous tooth root canal filling material is prepared by but not limited to emulsion solvent evaporation method, multiple emulsion solvent diffusion method, spray drying method, and multiple emulsion solvent evaporation method.
8. The method for preparing a PLGA nanoparticle deciduous tooth root canal filling material according to claim 1, comprising the steps of:
1) adding PLGA into an acetone solution, and stirring the solution at 750-1000 r/min to obtain a solution A;
2) pouring the solution A obtained in the step 1) into a polyvinyl alcohol solution, stirring, standing until acetone is volatilized, centrifuging, removing supernatant, cleaning the precipitate with deionized water, centrifuging again, taking the precipitate, performing ultrasonic mixing treatment, centrifuging again, taking the precipitate, and air-drying for later use to obtain the PLGA nano-particle deciduous tooth root canal filling material.
9. The method for preparing PLGA nanoparticle deciduous tooth root canal filling material according to claim 8, wherein in step 1), the ratio of the acetone solution to PLGA is (3-5 mL): 25-100 mg, wherein the acetone solution is calculated by volume and the PLGA is calculated by mass.
10. The method for preparing a PLGA nanoparticle deciduous tooth root canal filling material according to claim 8, wherein in the step 2), the polyvinyl alcohol solution is added in an amount of 20 to 80ml, and the concentration of the polyvinyl alcohol solution is 1.5 to 5%; the stirring time can be 6-10 h, and the mixture can be kept stand until acetone is volatilized and then centrifuged at 12000-20000 rpm for 12-20 min; the time of the ultrasonic uniform mixing treatment can be 15-40 s.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050079470A1 (en) * | 2003-10-10 | 2005-04-14 | Bruce Rutherford | Methods for treating dental conditions using tissue scaffolds |
| US20100316722A1 (en) * | 2000-07-07 | 2010-12-16 | Laboratorios Farmaceuticos Rovi S.A. | Pharmaceutical Forms for the Release of Active Compounds |
| US20110171607A1 (en) * | 2007-10-25 | 2011-07-14 | The Trustees Of Columbia University In The City Of New York | Biopulp |
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- 2019-10-31 CN CN201911053655.4A patent/CN110721093A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100316722A1 (en) * | 2000-07-07 | 2010-12-16 | Laboratorios Farmaceuticos Rovi S.A. | Pharmaceutical Forms for the Release of Active Compounds |
| US20050079470A1 (en) * | 2003-10-10 | 2005-04-14 | Bruce Rutherford | Methods for treating dental conditions using tissue scaffolds |
| US20110171607A1 (en) * | 2007-10-25 | 2011-07-14 | The Trustees Of Columbia University In The City Of New York | Biopulp |
Non-Patent Citations (2)
| Title |
|---|
| MASUO HOSOKAWA等: "《纳米粒子技术手册II》", 31 March 2015, 哈尔滨工业大学出版社 * |
| 许海燕等: "《纳米生物医学技术》", 30 June 2009 * |
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