CN110713911A

CN110713911A - In-situ microorganism collection system

Info

Publication number: CN110713911A
Application number: CN201910877646.0A
Authority: CN
Inventors: 盖英宝; 吴冬华
Original assignee: Hunan Puli Marine Science & Technology Co Ltd; Third Institute of Oceanography MNR
Current assignee: Hunan Puli Marine Science & Technology Co Ltd; Third Institute of Oceanography MNR
Priority date: 2019-09-17
Filing date: 2019-09-17
Publication date: 2020-01-21
Anticipated expiration: 2039-09-17
Also published as: CN110713911B

Abstract

The invention discloses an in-situ microorganism collecting system which comprises a first one-way valve, a pure water tank, a three-way valve, a filtering device, a pipeline, a second one-way valve, a deep water pump, a buffer solution adding device and an energy storage type single-action hydraulic cylinder, wherein one end of the first one-way valve is connected with a seawater inlet end, the other end of the first one-way valve is connected with the pure water tank, two water inlet ends of the three-way valve are respectively connected with the pure water tank and the buffer solution adding device, and the water outlet end of the three-way valve; the water outlet end of the filtering device is respectively connected with the second one-way valve, the energy storage type single-action hydraulic cylinder and the water inlet end of the deep water pump through pipelines, and the energy storage type single-action hydraulic cylinder is connected with the three-way valve and used for switching and controlling one of the two water inlet ends of the three-way valve to be opened. Starting the deep water pump, opening the first one-way valve, communicating the three-way valve with the pure water tank, and allowing seawater to enter the filtering device; and after filtration, the deep water pump is stopped, the first one-way valve is closed, the second one-way valve is opened, and the three-way valve is communicated with the buffer solution adding device so that the buffer solution automatically enters the filtering device.

Description

In-situ microorganism collection system

Technical Field

The invention relates to the field of deep sea microorganism in-situ sampling, in particular to a deep sea in-situ microorganism collecting system.

Background

With the development of deep sea exploration technology, people find that a large number of biological resources with various types and unique properties are stored in deep sea, and microorganisms are the most important type of the biological resources. The deep sea microorganisms survive in the extreme environment of deep sea, form special biological structures, gene types, physiological mechanisms and metabolic products, and have important scientific research value and economic value, so the deep sea microorganism resources are one of the most competitive fields of all countries in the world.

The deep-sea microorganisms exist in various forms in seawater, the types and characteristics of microorganisms in different water layers are greatly different due to the difference of important parameters such as illumination, temperature, pressure and the like, the required deep-sea microorganisms are obtained mainly by adopting a suction filtration mode in the conventional deep-sea microorganism collecting system, and exchange pollution of microorganisms in other water layers is easily caused in the collecting process, so that the cells of the collected microorganisms lack the original state in the seawater and the scientific research and economic values are lost.

Disclosure of Invention

The invention mainly aims to provide an in-situ microorganism collecting system, which solves the problem of exchange pollution of other water layer microorganisms in the deep sea microorganism collecting process, and can add specific buffer solution to the collected microorganism water body and a filtering membrane to preserve the original state of microorganism cells in seawater.

In order to achieve the purpose, the in-situ microorganism collecting system provided by the invention comprises a first one-way valve, a pure water tank, a three-way valve, a filtering device, a pipeline, a second one-way valve, a deep water pump, a buffer solution adding device and an energy storage type single-action hydraulic cylinder, wherein one end of the first one-way valve is connected with a seawater inlet end, the other end of the first one-way valve is connected with the pure water tank, two water inlet ends of the three-way valve are respectively connected with the pure water tank and the buffer solution adding device, and a water outlet end of the three-way valve is connected; the water outlet end of the filtering device is respectively connected with the starting end of the second one-way valve, the liquid inlet of the energy storage type single-action hydraulic cylinder and the water inlet end of the deep water pump through the pipeline; the energy storage type single-action hydraulic cylinder is used for storing energy in a negative pressure environment formed after the deep water pump is started, and after the deep water pump stops and negative pressure disappears, the stored energy is released to enable a piston rod of the energy storage type single-action hydraulic cylinder to push a valve core of the three-way valve, so that the water inlet ends of the pure water tank in the two water inlet ends of the three-way valve are switched and controlled to be closed, and the water inlet end of the buffer solution adding device is opened.

Preferably, the first one-way valve is installed at the top of the pure water tank, and the first one-way valve is a vacuum one-way valve.

Preferably, a container cavity is formed inside the filtering device, the container cavity is respectively communicated with the water inlet end of the filtering device and the water outlet end of the filtering device, a filter membrane is installed in the container cavity, and the filter membrane is arranged between the water inlet end of the filtering device and the water outlet end of the filtering device.

Preferably, the three-way valve comprises a single-action mechanism, and a first valve core and a second valve core which are connected with the single-action mechanism, wherein the first valve core is used for opening and closing the pure water tank inlet, and the second valve core is used for opening and closing the buffer solution adding device inlet; the single-action mechanism is started to drive the first valve core to be opened and the second valve core to be closed; the single-acting mechanism is stopped to drive the first valve core to close and the second valve core to open.

Preferably, the buffer solution adding device comprises a piston plate, a first spring and a piston cylinder for containing a buffer solution, the piston plate is hermetically installed in the piston cylinder to divide an inner cavity of the piston cylinder into a first space and a second space, the first space is connected with one water inlet end of the three-way valve through the pipeline, one end of the first spring is fixed on the wall surface of the second space far away from the first space, the other end of the first spring is connected with one side of the piston plate facing the second space, and the piston plate is in sliding connection with the inner wall of the piston cylinder body; the buffer solution adding device is used for compressing and storing energy through the first spring when buffer solution is added, after the deep water pump stops, the three-way valve is connected with the water inlet end of the buffer solution adding device and is opened, and the first spring extends to release energy to push the piston plate to compress the first space, so that the buffer solution automatically enters the filtering device.

Preferably, the hydraulic cylinder comprises a cylinder body, a second spring and a piston rod, the cylinder body is divided by the piston rod to form a rod cavity and a rodless cavity, and the piston rod is connected with the cylinder body in a sliding manner, so that a rod body of the piston rod extends or retracts towards the direction of the rodless cavity, which is far away from the rod cavity; the second spring is arranged in the rodless cavity along the sliding direction of the piston rod, the rodless cavity is connected with the water outlet end of the filtering device and the water inlet end of the deep water pump through the pipeline, and the piston head of the piston rod is connected with one end of the second spring; a contact is arranged in the moving direction of the piston rod, and the tail end of the rod body of the piston rod contracts away from the contact to start the single-action mechanism of the three-way valve; and the tail end of the rod body of the piston rod extends out of the abutting contact to stop the single-action mechanism of the three-way valve.

Preferably, the second one-way valve is located at the front end of the deep water pump, and the second one-way valve is a vacuum one-way valve.

In order to achieve the above object, the present invention further provides an in-situ microorganism collection method, which is performed by the in-situ microorganism collection system, and the in-situ microorganism collection method includes the following steps:

filling a pure water tank with pure water, filling a buffer solution adding device with buffer solution, and filling an energy storage type single-action hydraulic cylinder with pure water;

starting a deep water pump to pump out pure water in a pure water tank, forming negative pressure in a pipeline device connected to a water inlet end of the deep water pump, controlling a first one-way valve to be opened, closing a second one-way valve, starting energy storage of an energy storage type single-action hydraulic cylinder, and pumping out the pure water in the energy storage type single-action hydraulic cylinder to enable a piston rod of the hydraulic cylinder to be contracted; the water inlet end of the control three-way valve is communicated with the pure water tank so as to enable the seawater to enter the filtering device for filtering;

when a filtering completion signal is received, the deep water pump is controlled to stop working, the negative pressure disappears, the first one-way valve is closed, the energy storage type single-action hydraulic cylinder starts to release energy, the second spring is released, and the piston rod extends out; the pure water tank of three-way valve is intake and is held closed, and the buffer solution of three-way valve adds the device end of intaking and opens, and the end of intaking of three-way valve adds the device intercommunication so that buffer solution gets into filter equipment automatically with the buffer solution, and the second check valve is opened, and unnecessary water of discharge makes the buffer solution be full of the both ends of filter membrane in the filter equipment.

The three-way valve comprises a single-action mechanism, a first valve core and a second valve core, wherein the first valve core and the second valve core are connected with the single-action mechanism; the energy storage type single-action hydraulic cylinder comprises a cylinder body, a second spring and a piston rod, wherein the cylinder body is divided into a rod cavity and a rodless cavity by the piston rod, and the piston rod is in sliding connection with the cylinder body so that a rod body of the piston rod extends out or retracts towards the direction of the rodless cavity, which is far away from the rod cavity; the second spring is arranged in the rodless cavity along the sliding direction of the piston rod, and the rodless cavity is connected with the water outlet end of the filtering device and the water inlet end of the deep water pump through the pipeline;

the step of starting the deep water pump to pure water in the pure water jar is taken out, the deep water pump is intake and is formed the negative pressure in the pipeline spare that the end was taken over, and the first check valve of control is opened, and the second check valve is closed, and energy storage formula single action pneumatic cylinder begins the energy storage, takes out the pure water in the cylinder body of energy storage formula single action pneumatic cylinder so that energy storage formula single action pneumatic cylinder inner piston rod shrink includes:

starting a deep water pump to pump pure water in the pure water tank, and controlling the first one-way valve to be opened and the second one-way valve to be closed;

pure water in the cylinder body of the energy storage type single-action hydraulic cylinder is pumped out to enable a second spring in the cylinder body to compress and store energy, a piston rod is contracted to start a single-action mechanism of a three-way valve, and the single-action mechanism is started to drive a first valve core to be opened and a second valve core to be closed so as to enable seawater to enter a filtering device for filtering.

Preferably, when the filtering completion signal is received, the deep water pump is controlled to stop working, the negative pressure disappears, the first one-way valve is closed, the energy storage type single-action hydraulic cylinder starts to release energy, the second spring is released, and the piston rod extends out; the pure water tank of three-way valve is intake and is held closed, and the buffer solution of three-way valve adds the device and intakes and hold and open, and the end of intaking of three-way valve adds the device intercommunication so that buffer solution gets into filter equipment automatically, and the second check valve is opened, and unnecessary water of discharge makes the buffer solution be full of the step at the both ends of filter membrane in the filter equipment, includes:

when a filtering completion signal is received, controlling the deep water pump to stop working;

the first one-way valve is closed, the second one-way valve is opened, so that seawater enters an inner cavity of a cylinder body of the energy storage type single-action hydraulic cylinder through the three-way valve and the filtering device, a second spring in the cylinder body releases energy to extend, the single-action mechanism stops to drive the first valve core to be closed and the second valve core to be opened, the first spring in a piston cylinder of the buffer solution adding device releases energy to extend, so that buffer solution enters the filtering device and fills two ends of a filter membrane in the filtering device, and redundant buffer solution is discharged through the second one-way valve at the front end of the deep water pump.

According to the technical scheme, one end of the first one-way valve is connected with the seawater inlet end, the other end of the first one-way valve is connected with the pure water tank, when the deep water pump works, the first one-way valve is opened, the second one-way valve is closed, the water inlet end of the three-way valve is communicated with the pure water tank, and pure water in the pure water tank is pumped out, so that seawater enters the filtering device to be filtered, and the filtering device is prevented from being polluted by microbial exchange of other water layers. After the filtration is finished, when the deep water pump stops working, the first one-way valve is closed, the second one-way valve is opened, the filtered seawater enters the hydraulic cylinder to enable the piston rod to extend, the water inlet end of the control three-way valve is communicated with the buffer solution adding device, the buffer solution in the buffer solution adding device is automatically added into the filtering device, a specific buffer solution is added to the collected microbial water body and the filtering filter membrane, and the in-situ microbial collection system for preserving the original state of microbial cells in the seawater is provided.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the structures shown in the drawings without creative efforts.

Fig. 1 is a schematic structural diagram according to an embodiment of the present invention.

FIG. 2 is a schematic diagram of the collection and filtering process according to an embodiment of the present invention (the thick line in the figure represents the flow direction).

FIG. 3 is a schematic diagram of automatic buffer addition according to an embodiment of the present invention (the thick line in the figure represents the flow direction).

The reference numbers illustrate:

reference numerals	Name (R)	Reference numerals	Name (R)
				1	First check valve	2	Pure water tank
3	Three-way valve	31	Single-action mechanism 31
				32	First valve core	33	Second valve core
4	Filter device	41	Filter membrane
				6	Second check valve	7	Buffer solution adding device
71	Piston cylinder	72	Piston plate
				73	First spring	74	The first space
75	Second space	8	Energy storage type single-action hydraulic cylinder
				81	Cylinder body	82	Second spring
83	Piston rod	9	Deep water pump
				10	Flow meter

The implementation, functional features and advantages of the objects of the present invention will be further explained with reference to the accompanying drawings.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

It should be noted that all the directional indicators (such as up, down, left, right, front, and rear … …) in the embodiment of the present invention are only used to explain the relative position relationship between the components, the movement situation, etc. in a specific posture (as shown in the drawing), and if the specific posture is changed, the directional indicator is changed accordingly.

In addition, the descriptions related to "first", "second", etc. in the present invention are only for descriptive purposes and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

In the present invention, unless otherwise expressly stated or limited, the terms "connected," "secured," and the like are to be construed broadly, and for example, "secured" may be a fixed connection, a removable connection, or an integral part; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

In addition, the technical solutions in the embodiments of the present invention may be combined with each other, but it must be based on the realization of those skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination of technical solutions should not be considered to exist, and is not within the protection scope of the present invention.

The invention provides an in-situ microorganism collecting system.

With reference to fig. 1 to 3, the deep sea in-situ microorganism collection system disclosed in this embodiment includes a first check valve 1, a pure water tank 2, a three-way valve 3, a filtering device 4, a pipeline 5, a second check valve 6, a buffer solution adding device 7, an energy storage type single-acting hydraulic cylinder 8 and a deep water pump 9, wherein one end of the first check valve 1 is connected to a seawater inlet end, the other end of the first check valve is connected to the pure water tank 2, two water inlet ends of the three-way valve 3 are respectively connected to the pure water tank and the buffer solution adding device, and a water outlet end of the three-way valve 3 is connected to a water inlet end of the filtering device; the water outlet end of the filtering device 4 is respectively connected with the starting end of the second one-way valve 2, the liquid inlet of the hydraulic cylinder 2 and the water inlet end of the deep water pump 9 through the pipeline 5; the energy storage type single-action hydraulic cylinder 8 is used for storing energy in a negative pressure environment formed after the deep water pump 9 is started, and after the deep water pump 9 stops and negative pressure disappears, the stored energy is released to enable a piston rod 83 of the energy storage type single-action hydraulic cylinder 8 to push a valve core of the three-way valve 3, so that the water inlet ends of the pure water tank 2 in the two water inlet ends of the three-way valve are switched and controlled to be closed, and the water inlet end of the buffer solution adding device 7 is opened.

According to the technical scheme, one end of a first one-way valve 1 is connected with a seawater inlet end, the other end of the first one-way valve is connected with a pure water tank 2, when a deep water pump 9 works, the first one-way valve 1 is opened, a second one-way valve 6 is closed, the water inlet end of a three-way valve 3 is communicated with the pure water tank 2, and pure water in the pure water tank 2 is pumped out, so that seawater enters a filtering device 4 to be filtered, and the filtering device 4 is prevented from being polluted by exchange of microorganisms in other water layers. When the deep water pump 9 stops working after the filtration is completed, the first check valve 1 is closed, the second check valve 6 is opened, the filtered seawater enters the energy storage type single-action hydraulic cylinder 8 to extend the piston rod 83, the water inlet end of the control three-way valve 3 is communicated with the buffer solution adding device 7, and the buffer solution in the buffer solution adding device 7 is automatically added into the filtering device 4.

Preferably, the first check valve 1 is installed at the top of the pure water tank 2, the first check valve 1 is a vacuum check valve, and the first check valve 1 is opened when negative pressure exists in the tank body of the pure water tank 2; when the pure water tank 2 is filled with pure water, negative pressure does not exist in the tank body of the pure water tank 2, and the first check valve 1 is closed under the action of the spring in the first check valve. The inner cavity of the filter device 4 can be prevented from being polluted by other water layer microorganisms through the matching of the first one-way valve 1 and the pure water tank 2.

Preferably, the three-way valve 3 comprises a single-acting mechanism 31 and a first valve core 32 and a second valve core 33 which are connected with the single-acting mechanism 31, wherein the first valve core 32 is used for opening and closing the inlet of the pure water tank 2, and the second valve core 33 is used for opening and closing the inlet of the buffer adding device 7; the single-action mechanism 31 is started to drive the first valve core 32 to open and the second valve core 33 to close; the single-acting mechanism 31 is stopped to bring the first valve spool 32 closed and the second valve spool 33 open. In the initial state before the filtration of the collection system, the pure water tank 2 is filled with pure water, when the deep water pump 9 is started, the single-action mechanism 31 is started, and when the deep water pump 9 is stopped, the single-action mechanism 31 is stopped.

Preferably, a container cavity is formed inside the filtering device 4, the container cavity is respectively communicated with the water inlet end of the filtering device 4 and the water outlet end of the filtering device, a filter membrane 41 is installed in the container cavity, and the filter membrane 41 is arranged between the water inlet end of the filtering device 4 and the water outlet end of the filtering device 4. The seawater in the deep sea passes through the first check valve 1 and then passes through the water inlet end of the filtering device 4, and the microorganisms in the seawater are intercepted on the filter screen 41.

Preferably, the buffer solution adding device 7 comprises a piston plate 72, a first spring 73 and a piston cylinder 71 for containing a buffer solution, wherein the piston plate 72 is hermetically installed in the piston cylinder 71 to divide the inner cavity of the piston cylinder into a first space 74 and a second space 75, the first space 74 is connected with one water inlet end of the three-way valve 3 through the pipeline 5, one end of the first spring 73 is fixed on the wall surface of the second space 75 far away from the first space 74, the other end of the first spring 73 is connected with one side of the piston plate 72 facing the second space 75, and the piston plate 72 is in sliding connection with the wall of the inner cavity of the piston cylinder 71. When the buffer solution adding device 7 adds the buffer solution, the first spring 73 is compressed to store energy, after the deep water pump 9 is stopped, the three-way valve 3 is connected with the water inlet end of the buffer solution adding device 7 to be opened, and the first spring 73 is extended to release energy to push the piston plate 72 to compress the first space 74, so that the buffer solution automatically enters the filtering device 4. In an initial state before filtration of the collection system, the piston cylinder 71 of the buffer solution adding device 7 is filled with buffer solution, the first spring 73 is compressed to store energy, and the outlet of the buffer solution adding device 7 is closed by the second valve core 33 of the three-way valve 3.

Preferably, the energy-storage single-acting hydraulic cylinder 8 comprises a cylinder body 81, a second spring 82 and a piston rod 83, the piston rod 83 divides the cylinder body into a rod cavity and a rodless cavity, and the piston rod 83 is slidably connected with the cylinder body, so that a rod body 832 of the piston rod 83 extends or retracts towards the direction of the rodless cavity, which is far away from the rod cavity; the second spring 82 is arranged in the rodless cavity along the sliding direction of the piston rod 83, the rodless cavity is connected with the water outlet end of the filtering device 4 and the water inlet end of the deep water pump 9 through the pipeline 5, and the piston head 831 of the piston rod 83 is connected with one end of the second spring 82; a contact point F is arranged in the moving direction of the piston rod 83, and the end of the rod body 832 of the piston rod 83 is contracted away from the contact point F to start the single-action mechanism 31 of the three-way valve 3; the end of the rod 832 of the piston rod 83 protrudes beyond the abutment F to stop the single-action mechanism 31 of the three-way valve 3. In an initial state before filtration of the collection system, pure water is filled in a rodless cavity corresponding to the cylinder body 81 of the energy storage type single-action hydraulic cylinder 8, when the deep water pump 9 is started, the pure water in the cylinder body 81 is pumped away, the tail end of the rod body 832 of the piston rod 83 is far away from the contact F, and the single-action mechanism 31 is started.

Preferably, the second check valve 6 is located at the front end of the deep water pump 9, the second check valve 6 is a vacuum check valve, the second check valve 6 is closed under the action of negative pressure, and the second check valve 6 is opened under the action of an internal spring of the second check valve 6 under the condition that negative pressure does not exist.

Further, the in-situ microorganism collecting system of this embodiment further includes a flow meter 10, the flow meter 10 is installed on the water outlet end of the deep water pump 9, the flow meter 10 is used for measuring the flow passing through the deep water pump 9 during the filtration of the collecting system, and the deep water pump 9 stops working after the filtration flow reaches a set value.

The collection method of the in-situ microorganism collection system comprises the following steps:

filling pure water into the pure water tank 2, filling the buffer solution into the piston cylinder 71 of the buffer solution adding device 7, and filling the rodless cavity of the energy storage type single-action hydraulic cylinder 8 with pure water;

starting the deep water pump 9 to pump out pure water in the pure water tank 2, forming negative pressure in a pipeline device connected to the water inlet end of the deep water pump 9, controlling the first one-way valve 1 to be opened, closing the second one-way valve 6, starting energy storage of the energy storage type single-action hydraulic cylinder 8, and pumping out the pure water in the energy storage type single-action hydraulic cylinder 8 to enable a piston rod 83 of the energy storage type single-action hydraulic cylinder 8 to contract; the water inlet end of the control three-way valve 3 is communicated with the pure water tank 2, so that the seawater enters the filtering device 4 for filtering;

when a filtering completion signal is received, the deep water pump 9 is controlled to stop working, the negative pressure disappears, the first one-way valve 1 is closed, the energy storage type single-action hydraulic cylinder 8 starts to release energy, the second spring 82 is released to extend, and the piston rod 83 extends out; the end closure is intake to three-way valve 3's pure water tank 2, and three-way valve 3's buffer solution adds device 9 and intakes the end and opens, and three-way valve 3's the end of intaking adds device 9 intercommunication so that the buffer solution gets into filter equipment 4 automatically, and second check valve 6 is opened, and unnecessary water of discharge makes the buffer solution be full of the both ends of filter membrane 41 in filter equipment 4.

Further, the three-way valve 3 comprises a single-acting mechanism 31 and a first valve core 32 and a second valve core 33 which are connected with the single-acting mechanism 31, wherein the first valve core 32 is used for opening and closing an inlet of the pure water tank 2, and the second valve core 33 is used for opening and closing an inlet of the buffer solution adding device 7; the energy-storage single-action hydraulic cylinder 8 comprises a cylinder body 81, a second spring 82 and a piston rod 83, the cylinder body is divided by the piston rod 83 to form a rod cavity and a rodless cavity, and the piston rod 83 is connected with the cylinder body 82 in a sliding mode, so that a rod body 832 of the piston rod 83 extends or retracts towards the direction of the rodless cavity, which is far away from the rod cavity; the second spring 82 is arranged in the rodless cavity along the sliding direction of the piston rod 83, and the rodless cavity is connected with the water outlet end of the filtering device 4 and the water inlet end of the deep water pump 9 through the pipeline 5;

preferably, the second step includes:

starting a deep water pump 9 to pump pure water out of the pure water tank 2, forming negative pressure in a pipeline device connected to a water inlet end of the deep water pump 9, and controlling the first one-way valve 1 to be opened and the second one-way valve 6 to be closed; the energy storage type single-acting hydraulic cylinder 8 starts to store energy, pure water in a cylinder body 81 of the energy storage type single-acting hydraulic cylinder 8 is pumped out to enable a second spring 82 in the cylinder body 81 to compress and store energy, a piston rod 83 contracts to start a single-acting mechanism 31 of the three-way valve 3, and the single-acting mechanism 31 is started to drive the first valve core 32 to be opened and the second valve core 33 to be closed so as to enable seawater to enter the filtering device 4 for filtering.

Preferably, the third step includes:

when a filtering completion signal sent by the flowmeter 10 is received, the deep water pump 9 is controlled to stop working, and the negative pressure disappears;

the first check valve 1 is closed, the second check valve 6 is opened, so that seawater enters an inner cavity of a cylinder body 81 of the energy storage type single-action hydraulic cylinder 8 through the three-way valve 3 and the filtering device 4, a second spring 82 in the cylinder body 81 releases energy to extend, the single-action mechanism 31 stops to drive the first valve core 32 to be closed and the second valve core 33 to be opened, the first spring 73 in a piston cylinder 71 of the buffer solution adding device 7 releases energy to extend, so that buffer solution enters the filtering device 4 and fills two ends of a filter membrane 41 in the filtering device 4, and redundant buffer solution is discharged through the second check valve 6 at the front end of the deep water pump 9.

The above description is only a preferred embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications and equivalents of the present invention, which are made by the contents of the present specification and the accompanying drawings, or directly/indirectly applied to other related technical fields, are included in the scope of the present invention.

Claims

1. An in-situ microorganism collection system is characterized by comprising a first one-way valve, a pure water tank, a three-way valve, a filtering device, a pipeline, a second one-way valve, a deep water pump, a buffer solution adding device and an energy storage type single-action hydraulic cylinder, wherein one end of the first one-way valve is connected with a seawater inlet end, the other end of the first one-way valve is connected with the pure water tank, two water inlet ends of the three-way valve are respectively connected with the pure water tank and the buffer solution adding device, and a water outlet end of the three-way valve is connected with a water inlet end; the water outlet end of the filtering device is respectively connected with the starting end of the second one-way valve, the liquid inlet of the energy storage type single-action hydraulic cylinder and the water inlet end of the deep water pump through the pipeline; the energy storage type single-action hydraulic cylinder is used for storing energy in a negative pressure environment formed after the deep water pump is started, and after the deep water pump stops and negative pressure disappears, the stored energy is released to enable a piston rod of the energy storage type single-action hydraulic cylinder to push a valve core of the three-way valve, so that the water inlet ends of the pure water tank in the two water inlet ends of the three-way valve are switched and controlled to be closed, and the water inlet end of the buffer solution adding device is opened.

2. The in situ microorganism collection system of claim 1, wherein the first one-way valve is mounted on the top of the purified water tank, the first one-way valve being a vacuum one-way valve.

3. The in situ microorganism collection system of claim 1, wherein the filter device defines a chamber therein, the chamber being in communication with the inlet end of the filter device and the outlet end of the filter device, the chamber having a filter membrane disposed therein, the filter membrane being disposed between the inlet end of the filter device and the outlet end of the filter device.

4. The in situ microorganism collection system of claim 2, wherein the three-way valve comprises a single-acting mechanism and a first valve core and a second valve core connected with the single-acting mechanism, wherein the first valve core is used for opening and closing the pure water tank inlet, and the second valve core is used for opening and closing the buffer solution adding device inlet; the single-action mechanism is started to drive the first valve core to be opened and the second valve core to be closed; the single-acting mechanism is stopped to drive the first valve core to close and the second valve core to open.

5. The in situ microorganism collection system of claim 4, wherein the buffer solution adding device comprises a piston plate, a first spring and a piston cylinder for containing the buffer solution, the piston plate is hermetically mounted in the piston cylinder to divide the inner cavity of the piston cylinder into a first space and a second space, the first space is connected with one water inlet end of the three-way valve through the pipeline, one end of the first spring is fixed on the wall surface of the second space far away from the first space, the other end of the first spring is connected with one side of the piston plate facing the second space, and the piston plate is connected with the inner wall of the piston cylinder in a sliding manner; the buffer solution adding device is used for compressing and storing energy through the first spring when buffer solution is added, after the deep water pump stops, the three-way valve is connected with the water inlet end of the buffer solution adding device and is opened, and the first spring extends to release energy to push the piston plate to compress the first space, so that the buffer solution automatically enters the filtering device.

6. The in situ microorganism collection system of claim 4, wherein the energy accumulating single acting hydraulic cylinder comprises a cylinder body, a second spring and a piston rod, the piston rod divides the cylinder body into a rod cavity and a rodless cavity, the piston rod is slidably connected with the cylinder body, so that a rod body of the piston rod extends or retracts in a direction of the rodless cavity, which is away from the rod cavity; the second spring is arranged in the rodless cavity along the sliding direction of the piston rod, the rodless cavity is connected with the water outlet end of the filtering device and the water inlet end of the deep water pump through the pipeline, and the piston head of the piston rod is connected with one end of the second spring; a contact is arranged in the moving direction of the piston rod, and the tail end of the rod body of the piston rod contracts away from the contact to start the single-action mechanism of the three-way valve; and the tail end of the rod body of the piston rod extends out of the abutting contact to stop the single-action mechanism of the three-way valve.

7. The in situ microorganism collection system of claim 6, wherein the second one-way valve is located at a front end of the deep water pump, the second one-way valve being a vacuum one-way valve.

8. An in situ microorganism collection method applied to the collection system of any one of claims 1 to 7, comprising the steps of:

9. The in-situ microorganism collecting method according to claim 8, wherein the three-way valve comprises a single-acting mechanism and a first valve core and a second valve core which are connected with the single-acting mechanism, the first valve core is used for opening and closing a pure water tank inlet, and the second valve core is used for opening and closing a buffer adding device inlet; the energy storage type single-action hydraulic cylinder comprises a cylinder body, a second spring and a piston rod, wherein the cylinder body is divided into a rod cavity and a rodless cavity by the piston rod, and the piston rod is in sliding connection with the cylinder body so that a rod body of the piston rod extends out or retracts towards the direction of the rodless cavity, which is far away from the rod cavity; the second spring is arranged in the rodless cavity along the sliding direction of the piston rod, and the rodless cavity is connected with the water outlet end of the filtering device and the water inlet end of the deep water pump through the pipeline;

10. The in-situ microorganism collection method according to claim 9, wherein when the filtration completion signal is received, the deep water pump is controlled to stop working, the negative pressure disappears, the first one-way valve is closed, the energy storage type single-action hydraulic cylinder starts to release energy, the second spring is released, and the piston rod extends out; the pure water tank of three-way valve is intake and is held closed, and the buffer solution of three-way valve adds the device and intakes and hold and open, and the end of intaking of three-way valve adds the device intercommunication so that buffer solution gets into filter equipment automatically, and the second check valve is opened, and unnecessary water of discharge makes the buffer solution be full of the step at the both ends of filter membrane in the filter equipment, includes: