CN110713507A - Water extract powder of fresh celery leaf, its preparation, free radical scavenging action and application - Google Patents
Water extract powder of fresh celery leaf, its preparation, free radical scavenging action and application Download PDFInfo
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- CN110713507A CN110713507A CN201810765516.3A CN201810765516A CN110713507A CN 110713507 A CN110713507 A CN 110713507A CN 201810765516 A CN201810765516 A CN 201810765516A CN 110713507 A CN110713507 A CN 110713507A
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- 235000010591 Appio Nutrition 0.000 title claims abstract description 81
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- 230000007760 free radical scavenging Effects 0.000 title abstract description 3
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H17/00—Compounds containing heterocyclic radicals directly attached to hetero atoms of saccharide radicals
- C07H17/04—Heterocyclic radicals containing only oxygen as ring hetero atoms
- C07H17/06—Benzopyran radicals
- C07H17/065—Benzo[b]pyrans
- C07H17/07—Benzo[b]pyran-4-ones
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
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- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C67/00—Preparation of carboxylic acid esters
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- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C69/00—Esters of carboxylic acids; Esters of carbonic or haloformic acids
- C07C69/66—Esters of carboxylic acids having esterified carboxylic groups bound to acyclic carbon atoms and having any of the groups OH, O—metal, —CHO, keto, ether, acyloxy, groups, groups, or in the acid moiety
- C07C69/73—Esters of carboxylic acids having esterified carboxylic groups bound to acyclic carbon atoms and having any of the groups OH, O—metal, —CHO, keto, ether, acyloxy, groups, groups, or in the acid moiety of unsaturated acids
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- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
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- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H13/00—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids
- C07H13/02—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids
- C07H13/04—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids having the esterifying carboxyl radicals attached to acyclic carbon atoms
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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Abstract
The invention discloses a method for preparing fresh celery leaf water extract powder, discloses the structure types and names of all 34 components in the prepared water extract powder, and discloses that the 34 components are all new compounds; discloses that 3 of the 34 new compounds are sucrose compounds, 13 are quinic acid compounds, and 18 are apioside compounds; the invention discloses the free radical scavenging action of the prepared water extract powder, and therefore, the invention discloses the application of the fresh celery leaf water extract powder in preparing a medicament for preventing free radical damage.
Description
Technical Field
The invention relates to a method for preparing powder from an aqueous extract of fresh celery leaves, relating to the reproducibility of a process for preparing powder from the aqueous extract of the fresh celery leaves, which is defined by a characteristic UPLC-mass spectrum total ion current spectrum and 34 peaks thereof, relating to components in the powder, which are defined by the characteristic UPLC-mass spectrum total ion current spectrum and 34 peaks thereof, relating to 3 sucrose structures in the fresh celery leaf aqueous extract powder corresponding to the 34 peaks, relating to 13 quinic acid structures in the fresh celery leaf aqueous extract powder corresponding to the 34 peaks, relating to 18 apigenin structures in the fresh celery leaf aqueous extract powder corresponding to the 34 peaks, and further relating to the radical scavenging action of the fresh celery leaf aqueous extract powder, so that the invention relates to the application of the fresh celery leaf aqueous extract powder in preparing a medicament for preventing radical damage. The invention belongs to the field of biological medicine.
Background
A large number of statistical data indicate that damage caused by free radical accumulation, including damage caused by nitrogen free radical and hydroxyl free radical accumulation, is closely related to aging, low immune function of human body, atherosclerosis, ischemia reperfusion and cancer onset. Effective prevention of damage caused by the accumulation of nitrogen radicals and hydroxyl radicals has become a health concern worldwide. Although some vitamins and traditional Chinese medicines have the effects of clearing nitrogen free radicals and hydroxyl free radicals, the vitamins and the traditional Chinese medicines are still uncertain when being taken for a long time as preventive medicines. The discovery of the medicine which has no toxic side effect and can be used for preventing the damage caused by the accumulation of the nitrogen free radical and the hydroxyl free radical is an important direction of medicine research.
The folk always has a theory of cool nature and taste of the celery leaves, and the celery leaves are considered to have the effects of clearing heat and disinfecting, treating the exuberant liver fire and calming the liver and clearing heat. In folk, vegetable leaves are rich in cellulose, so that the theory of relaxing bowels and treating constipation is provided. In folks, celery leaves are also thought to have the effect of lowering blood pressure and also can treat dizziness, edema and irregular menstruation. Without rigorous pharmacodynamic studies, the basis of these statements is only experience. Furthermore, whether celery leaves in these parnassia are celery leaves or parsley leaves is not defined. Further, these statements are not directly related to vasodilation. In addition, the inventor proposes the liver protection effect of the fresh celery leaf nano juice. However, the fresh celery leaf nano juice is not only mildewed but also precipitated after being placed for 20 days. Mildew may cause disease and precipitation may alter chemical composition. These two fatal problems caused the inventors to abandon the subsequent development of the fresh celery leaf nano-juice.
The inventor conducts long-term research on chemical components and therapeutic effects of the water extract powder of the fresh celery leaves. The inventors have recognized that it is not clear to the skilled person to date which chemical components are contained in the aqueous extract of fresh celery leaves, nor is it clear what the therapeutic effect of the aqueous extract of fresh celery leaves is. In response to this situation, the inventors performed mass spectrometry on an aqueous extract of fresh celery leaves. Analysis of the 34 components in the aqueous extract of fresh celery leaves led the inventors to realise that the aqueous extract of fresh celery leaves scavenges both nitrogen and hydroxyl radicals. The structural analysis of 34 components in the water extract of the fresh celery leaves leads to the discovery of 34 new compounds, thereby embodying the intelligence and creativity of the invention. Compared with all celery related known inventions, the fresh celery leaf water extract powder achieves a brand new height. Based on these findings, the inventors have proposed the present invention.
Disclosure of Invention
The first content of the invention is a method for providing an extract powder of fresh celery leaves, which comprises the following steps:
(1) washing 1000g of fresh celery leaves without silt on the surface with 3000mL of tap water for 2 times, then transferring to a sterile environment, washing with 3000mL of triple-distilled water for 3 times, and filtering water to obtain clean fresh celery leaves;
(2) soaking clean fresh celery leaves in triple distilled water at 60-90 ℃ for 3 times, soaking in 3000mL of triple distilled water for at least 30 minutes each time, and slowly stirring during the soaking period;
(3) after each soaking, the water extract flows out slowly and automatically, and the time for use is not less than 30 minutes;
(4) and concentrating the obtained 9000mL of water extract at 60-90 deg.C under reduced pressure to obtain 24g of fresh celery leaf water extract powder.
The second content of the invention is to provide the ultraviolet spectrum of the fresh celery leaf extract powder.
The third content of the invention is to provide a mass spectrum total ion current spectrum of the fresh celery leaf extract powder.
A fourth aspect of the invention is to provide the chemical structures of the 34 components corresponding to the peaks in the mass spectrum total ion flow spectrum of the fresh celery leaf extract powder.
The fifth content of the invention is to describe the scavenging effect of the fresh celery leaf water extract powder on free radicals.
Drawings
FIG. 1 UPLC chromatogram of water extract powder of fresh celery leaf.
FIG. 2 shows UPLC-mass spectrum of total ion current of fresh celery leaf extract powder.
FIG. 3 shows the cleavage pathway of 6-O- [ E-3- (1,3,5,7, 9-dodecenylcarbonyloxy) -4-hydroxycinnamoyl ] sucrose.
FIG. 4 is a mass spectrum of 6-O- [ E-3- (1,3,5,7, 9-dodecapentaenecarbonyloxy) -4-hydroxycinnamoyl ] sucrose.
FIG. 5 is a schematic representation of the cleavage pathway of 3-O- [ E-3, 5-bis (carboxy-n-acryloyloxy) -4-formyloxy-cinnamoyl ] quinic acid.
FIG. 6 is a mass spectrum of 3-O- [ E-3, 5-bis (carboxy-n-acryloyloxy) -4-formyloxy-cinnamoyl ] quinic acid.
FIG. 7.3-n-hexylcarbonyloxy-n-heptanoyl apigenin cleavage pathway.
FIG. 8.3-Mass Spectrometry of n-hexylcarbonyloxy-n-heptanoyl apigenin.
Figure 9 concentration dependent scavenging of nitrogen radicals by the aqueous extract powder of fresh celery leaves.
Figure 10 concentration dependent scavenging of hydroxyl radicals of an aqueous extract powder of fresh celery leaves.
Detailed Description
To further illustrate the invention, a series of examples are given below. These examples are purely illustrative and are intended to be a detailed description of the invention only and should not be taken as limiting the invention.
Example 1 preparation of an aqueous extract powder of fresh celery leaves
1000g of fresh celery leaves without silt on the surface are washed with 3000mL of tap water for 2 times, and then transferred to a sterile environment and washed with 3000mL of triple-distilled water for 3 times. Filtering off water to obtain clean fresh celery leaves. Soaking clean fresh celery leaves in triple distilled water at 60-90 ℃ for 3 times, soaking in 3000mL of triple distilled water for at least 30 minutes each time, and slowly stirring during soaking. After each soaking, the water extract is allowed to slowly flow out by itself for no less than 30 minutes. About 9000mL of the slowly eluting aqueous extracts were combined and concentrated at 60-90 deg.C under reduced pressure to give 24g of powder of the aqueous extract of fresh celery leaves.
Example 2 determination of chromatography and Mass Spectrometry ion Spectroscopy of fresh celery leaf extract powder
2-1 preparation of sample solution (10mg/mL)
26.7mg of the powder of the water extract of fresh celery leaf was weighed out and dissolved in 2.67mL of ultrapure water. The resulting solution was sonicated for 1 minute, followed by centrifugation at 13000r/min for 10 minutes. The supernatant was taken, filtered through a 0.22 μm filter and placed in a sample vial for chromatographic and mass spectrometric determination.
2-2. chromatographic conditions
Chromatographic column Waters, Acquity
A HSS T3 column (2.1 × 100mm i.d.,1.8 μm);
the sample injection volume is 2 mu L;
PDA detector 210-400 nm;
mobile phase water (0.1% formic acid), acetonitrile;
the mobile phase washed the column according to the gradient of table 1.
TABLE 1 mobile phase gradiometer
2-3 measuring chromatogram
UPLC chromatogram of the water extract powder of fresh celery leaf was determined and recorded according to the above chromatographic determination conditions (see the attached figure 1 of the specification).
2-4. conditions for measuring ion flow spectrum and mass spectrum
Electrospray ionization mode, Positive (PI) and Negative (NI) modes;
the collision energy of the inclined trap is 20-60V;
cone current 40V;
the temperature of the ion source is 100 ℃;
the capillary voltage is 30 kV;
spraying pressure is 6.5 bar;
the m/z range is 100-1500.
2-5, recording ion flow spectrum and mass spectrum
The ion flow spectrum of the water extract powder of fresh celery leaves was determined and recorded according to the above conditions (see the attached figure 2 of the specification).
Example 3 Structure of 34 Components in an extract powder of fresh celery leaves was specified
The UPLC chromatogram of example 2 was coupled to a mass spectrum and the UPLC-mass spectrum of the fresh celery leaf extract powder was determined. The mass spectrometry conditions are two modes of electrospray ionization, positive and negative ions. The inclined trap collision energy is 20-60V. The cone voltage is 40V. The ion source temperature was 100 ℃. The capillary voltage was 30 kV. The pressure of the atomizer was 6.5 bar. The mass/charge ratio ranges from 100 to 1500. 34 independent peaks separated within 14 minutes. According to the mass spectrometry fragmentation law, the peaks (numbered sequentially from left to right for the peaks of the total ion current spectrum) are assigned to the corresponding components of table 2.
TABLE 2 component names corresponding to peaks of the Total ion flux map
Example 4 Mass Spectrometry, chemical Structure and lysis of 34 Components in fresh celery leaf extract powder
The molecular ions and the fragment ions were correlated with each other on the basis of the mass spectrum corresponding to each peak in the total ion current measured in analytical example 2. Thus, the process is completed. The inventors established the cleavage pathway of the 34 components in table 2 of example 3 under the mass spectrometric conditions. These cleavage pathways in turn are the basis for the discovery of the invention that 34 components in the water extract powder of fresh celery leaves have undisputed novelty and inventive step. As the cleavage pathway and mass spectrum of the 34 components under the mass spectrum conditions, FIG. 3 and FIG. 4 are the cleavage pathway and mass spectrum of 6-O- [ E-3- (1,3,5,7, 9-dodecenylcarbonyloxy) -4-hydroxycinnamoyl ] sucrose, FIG. 5 and FIG. 6 are the cleavage pathway and mass spectrum of 3-O- [ E-3, 5-bis (carboxy-n-propionyloxy) -4-formyloxy-cinnamoyl ] quinic acid, and FIG. 7 and FIG. 8 are the cleavage pathway and mass spectrum of 3-n-hexylcarbonyloxy-n-heptanoylapigenin, respectively.
Example 5 determination of nitrogen radical scavenging action of fresh celery leaf extract powder
In order to examine the nitrogen radical scavenging effect of the fresh celery leaf extract powder, the activity of the aqueous solution of the fresh celery leaf extract powder for scavenging the nitrogen radicals, namely 1,1-diphenyl-2- (2,4, 6-trinitrophenyl) hydrazine radical (DPPH) at different concentrations is measured on a paramagnetic resonance instrument according to a standard method. Specific procedures included preparation of a solution of DPPH in dioxane (0.8g/mL) and a solution of fresh celery leaf extract powder in ultrapure water (2mg/mL,4mg/mL,6mg/mL,8mg/mL,10mg/mL and 15 mg/mL). And adding 10 mu L of dioxane solution of the LDPPH and 10 mu L of ultrapure water into a 0.5mL EP tube, and uniformly mixing by vortex to obtain a sample to be detected. Sucking the sample by a capillary tube, adding the sample into a paramagnetic tube, sealing by using a sealing compound, incubating for 10 minutes at room temperature in a dark place, incubating for 10 minutes at room temperature in a light-exposed place, and repeating the incubation for 6 times on a paramagnetic resonance instrument to obtain a nitrogen free radical spectrum with the concentration of the ultrapure water solution of the fresh celery leaf water extract powder being zero. Adding 10 μ L of DPPH dioxane solution and 10 μ L of fresh celery leaf extract powder ultrapure water solution (2mg/mL,4mg/mL,6mg/mL,8mg/mL,10mg/mL and 15mg/mL) into a 0.5mL EP tube, and vortexing and mixing to obtain a sample to be detected. Sucking the sample by a capillary tube, adding the sample into a paramagnetic tube, sealing the tube by using a sealing glue, incubating the tube for 10 minutes at room temperature in a dark place, incubating the tube for 10 minutes at room temperature in a light place, and repeating the incubation for 6 times on a paramagnetic resonance instrument to obtain the nitrogen free radical spectra of the ultrapure water solution of the water extract powder of the fresh celery leaves with the concentrations of 2mg/mL,4mg/mL,6mg/mL,8mg/mL,10mg/mL and 15 mg/mL. The results in figure 9 demonstrate that the concentration of ultra pure aqueous solution of the fresh celery leaf extract powder reduces the nitrogen radical peak intensity dependently. Unlike clinically used vitamins and traditional Chinese medicines with nitrogen radical scavenging effect, fresh celery leaves are vegetables. As the vegetable which can be eaten at will, the water extract powder of the fresh celery leaves has the unexpected technical effect of removing the nitrogen free radical.
Example 6 determination of the hydroxyl radical scavenging action of an aqueous extract powder of fresh celery leaves
In order to examine the effect of removing the hydroxyl radical of the fresh celery leaf water extract powder, the activity of removing the hydroxyl radical of the aqueous solution of the fresh celery leaf water extract powder under different concentrations is measured on a paramagnetic resonance instrument according to a standard method. The specific operation comprises mixing 5 mu LFeSO4.7H2O aqueous solution (2.78mg/mL), 5. mu.L 5, 5-dimethyl-1-pyrroline oxide (5,5-dimethyl-1-pyridine-N-oxide, DMPO) aqueous solution (11.316mg/mL), and 5. mu. L H2O2The aqueous solution (1%) was vortexed and mixed in an EP tube. Sucking the sample by a capillary tube, adding the sample into a paramagnetic tube, sealing by using a sealing compound, incubating for 10 minutes at room temperature in a dark place, incubating for 10 minutes at room temperature in a light-exposed place, and repeating the incubation for 6 times on a paramagnetic resonance instrument to obtain a hydroxyl radical spectrum with the concentration of the ultrapure water solution of the fresh celery leaf water extract powder being zero. 5 mu LFeSO4 ·7H2O aqueous solution (2.78mg/mL), 5. mu.L DMPO aqueous solution (11.316mg/mL), 5. mu.L water solution of fresh celery leaf extract powder (0.5mg/mL,1.0mg/mL,2.0mg/mL and 6.0mg/mL) and 5. mu.LH2O2The aqueous solution (1%) was vortexed and mixed in an EP tube. Sucking the sample by a capillary tube, adding the sample into a paramagnetic tube, sealing the tube by using sealing glue, incubating the tube for 10 minutes at room temperature in a dark place, incubating the tube for 10 minutes at room temperature in a light place, and repeating the incubation for 6 times on a paramagnetic resonance instrument to obtain the hydroxyl radical spectrum of the ultrapure aqueous solution of the fresh celery leaf water extract powder with the concentration of 0.5mg/mL,1.0mg/mL,2.0mg/mL and 6.0 mg/mL. The results in fig. 10 illustrate that the concentration of ultra pure aqueous solution of the fresh celery leaf extract powder reduces the hydroxyl radical peak intensity dependently. Unlike clinically used vitamins and traditional Chinese medicines with hydroxyl radical scavenging effect, fresh celery leaves are vegetables. As a vegetable which can be eaten at will, the water extract powder of the fresh celery leaves has the unexpected technical effect of removing hydroxyl radicals.
Claims (7)
1. A powder of water extract of fresh celery leaf is provided.
2. A powder of an aqueous extract of fresh celery leaf according to claim 1, characterised in that it contains 34 components each of which is a novel compound.
3. 34 novel compounds according to claim 2, characterized in that 3 are 6-O-E-3, 4-dihydroxy-2-n-pentylcarbonyloxy cinnamoyl sucrose, 6-O-E-3-1,3,5,7, 9-dodecapentacenecarbonyloxy-4-hydroxycinnamoyl sucrose and 6-O-E-3, 5-di-n-pentyloxy-4-acryloylcarbonyloxy cinnamoyl sucrose.
4. The 34 novel compounds of claim 2, characterized in that 13 are 3-O-E-3, 5-dicarboxylic n-propylcarbonyloxy-4-acryloyloxy-cinnamoylquinic acid, 3-O-E-3, 5-dicarboxylic-4-n-pentenoyloxy-4-carboxypropenylcarbonyloxy-cinnamoylquinic acid, 3-O-E-3-2 ', 4' -pentadienoyloxy-4-acryloyloxy-cinnamoylquinic acid, 3-O-E-3-carboxylic n-pentanoyloxy-4-carbonylallyloxy-5-carboxylic n-pentenoyloxy-5-carboxylic n-pentenoylquinic acid
-1 ', 3' -acyloxycinnamoylquinic acid, 3-O-E-3, 5-dicarboxy n-hexanoyloxy-4-carboxy n-butyloxycarbonylquinic acid,
3-O-E-3, 5-dicarboxy-2 ', 4' -pentadienocarbonyloxy-4-propenylcarbonylcinnamoylquinic acid, 3-O-E-3, 5-dicarboxy-n-butanoyloxy-4-carboxypropionyloxycinnamoylquinic acid, 3-O-E-3, 5-dicarboxylacryloxy-4-1 '-hydroxy-2', 4 '-hexadienoyloxycinnamic acid, 3-O-E-3, 5-di-1' -carboxy-2 ', 4' -pentadienoyloxy-4-carboxy-n-propionyloxycinnamoylquinic acid, 3-O-E-3, 5-di-1 '-carboxy-1', 3 '-butadienoyloxy-4-carboxy-2', 4' -butadiene carbonyloxy cinnamoyl quinic acid, 3-O-E-3, 5-dicarboxymethylcarbonyloxy-4-hydroxyacetyl cinnamoyl quinic acid, 3-O-E-3, 5-dicarboxymenzyloxy-4-hydroxyacetyl cinnamoyl quinic acid, and 3-O- [ E-3, 5-dicarboxymenzyloxy-4-formyloxy cinnamoyl quinic acid.
5. The 34 novel compounds as claimed in claim 2, characterized in that 18 are 3-n-butyloxycarbonyloxapioside, 3-1 ', 3', 5 ', 7', 9 '-decapentaenecarbonyloxy-1', 3 ', 5', 7 ', 9' -decapentaenoylapioside, 3-2 ', 4', 6 '-heptatrienylcarbonyloxy-3', 5 '-hexadienoylapioside, 3-3' -butenylcarbonyloxypropyloylapioside, 3-acryloylcarbonyloxypapioside, 3-n-pentylcarbonyloxypapioside, 3-n-butylcarbonyloxypropionapioside, 3-2 ', 4', 6 '-heptatrienylcarbonyloxypropionoside, 3-n-propylcarbonyloxypapioside, 3-3', 5 '-hexadienoyloxycarbonyloxapioside, 3-2', 4 ', 6', 8 '-nontetraenylcarbonyloxy apigenin, 3-3', 5 ', 7' -octatrienylcarbonyloxy-3 ', 5' -hexadienyl acyl apigenin, 3-2 ', 4', 6 '-heptatrienylcarbonyloxy n-butyryl apigenin, 3-n-heptacarbonyloxy apigenin, 2, 4-pentadienyl acyl apigenin, 3-acetoxyapigenin, 3-5' -n-hexylenecarbonyloxy apigenin and 3-n-hexylcarbonyloxy n-heptanoyl apigenin.
6. A process for preparing an aqueous extract powder of fresh celery leaves according to claim 1, which process comprises the steps of:
A. washing 1000g of fresh celery leaves without silt on the surface with 3000mL of tap water for 2 times, then transferring to a sterile environment, washing with 3000mL of triple-distilled water for 3 times, and filtering water to obtain clean fresh celery leaves;
B. soaking clean fresh celery leaves in triple distilled water at 60-90 ℃ for 3 times, soaking in 3000mL of triple distilled water for at least 30 minutes each time, and slowly stirring during the soaking period;
C. after each soaking, the water extract flows out slowly and automatically, and the time for use is not less than 30 minutes;
D. concentrating the obtained 9000mL of water extract at 60-90 deg.C under reduced pressure to obtain about 24g of fresh celery leaf water extract powder;
E. the components contained in the aqueous extract powder are defined by the characteristic UPLC-mass spectrum total ion current spectrum and 34 peaks in the aqueous extract powder, and the mass of the aqueous extract powder is also defined by the characteristic UPLC-mass spectrum total ion current spectrum and 34 peaks in the aqueous extract powder.
7. Use of an aqueous extract powder of fresh celery leaf according to claim 1 for the manufacture of a medicament for the prevention of damage to nitrogen radicals and hydroxyl radicals.
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