CN110699395A - Method for preparing polyaniline by laccase catalysis - Google Patents
Method for preparing polyaniline by laccase catalysis Download PDFInfo
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- CN110699395A CN110699395A CN201911071007.1A CN201911071007A CN110699395A CN 110699395 A CN110699395 A CN 110699395A CN 201911071007 A CN201911071007 A CN 201911071007A CN 110699395 A CN110699395 A CN 110699395A
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- laccase
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/001—Amines; Imines
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Abstract
The invention belongs to the technical field of enzyme catalysis, and particularly relates to a method for preparing polyaniline through laccase catalysis. The invention screens laccase from different fungal species, finds that the crude enzyme solution of the sweet lucid ganoderma containing the laccase can efficiently catalyze aniline polymerization to prepare polyaniline, and obviously improves the catalytic activity of the laccase compared with that of pleurotus eryngii, coprinus comatus, AS bacteria, AC bacteria, russula or oyster mushroom sources.
Description
Technical Field
The invention belongs to the technical field of enzyme catalysis, and particularly relates to a method for preparing polyaniline through laccase catalysis.
Background
Polyaniline is a conductive polymer material and is applied to electronic products, luminescent materials, preservatives, antistatic property and the like. Polyaniline is obtained through the oxidative polymerization reaction of the scheme, generally, an oxidant is added into an acidic aqueous solution of aniline to react to obtain polyaniline precipitate, and the polyaniline product is obtained through subsequent steps of filtering, washing, drying, grinding and the like.
Enzyme catalysis is milder than chemical synthesis conditions, Nanjing university of physiology and engineering CN105244510B discloses a method for electropolymerizing aniline by a potentiostatic method while catalyzing laccase, but the patent does not disclose laccase source. The laccase used for synthesizing polyaniline has different sources and larger difference of the synthesizing effect.
According to the invention, on the basis of CN105244510B, the influence of laccase source on aniline polymerization is researched, so that the laccase with excellent catalytic performance is obtained.
Disclosure of Invention
According to the invention, through screening different fungi, the laccase with a good polyaniline synthesis effect is finally found, and surprisingly, the laccase from ganoderma can efficiently catalyze aniline polymerization.
The invention realizes the aim by the following technical scheme that the laccase is derived from ganoderma; further preferably sweet Ganoderma of Ganoderma.
The method for preparing polyaniline by laccase catalysis has the further technical scheme that: adding aniline, sodium dodecyl benzene sulfonate and crude enzyme solution of sweet lucid ganoderma containing laccase into the aqueous solution, then adding acetic acid buffer solution to adjust the pH value of the system to 3.0 to form a reaction system, and then standing at room temperature for reaction to obtain the ganoderma lucidum polysaccharide.
Preferably, 0.3-0.5mL of crude enzyme solution of sweet lucid ganoderma containing laccase, 10mmol/L aniline concentration and 20 mmol/L Sodium Dodecyl Benzene Sulfonate (SDBS) concentration are added into each 4mL reaction system.
The polyaniline prepared by the laccase-catalyzed aniline polymerization method can be used as an immobilized enzyme carrier.
The invention screens laccase from different fungal species, finds that the crude enzyme solution of the sweet lucid ganoderma containing the laccase can efficiently catalyze aniline polymerization to prepare polyaniline, and obviously improves the catalytic activity of the laccase compared with that of pleurotus eryngii, coprinus comatus, AS bacteria, AC bacteria, russula or oyster mushroom sources.
Drawings
FIG. 1 is a diagram showing the color change of the reaction system of laccase from different fungal species.
FIG. 2 is a full wavelength scan of a laccase catalyzed aniline polymerization system from different fungal species.
Detailed Description
The preparation method of crude laccase enzyme solutions of different fungal species is as follows according to the prior art, 2016 and 39 (8): 1692 to 1695.
Examples
Laccase from different fungal species is adopted to catalyze aniline polymerization, a 5ml centrifuge tube is used as a reactor, as shown in figure 1,
adding aniline, sodium dodecyl benzene sulfonate and laccase crude enzyme liquid 0.4ml from different sources into a centrifugal tube, supplementing purified water to 4ml, then adding acetic acid buffer solution to adjust the pH value of the system to 3.0 to form a reaction system, and standing at room temperature for 24h to obtain the laccase-containing compound enzyme preparation; the aniline concentration in the reaction system is 10mmol/L, and the Sodium Dodecyl Benzene Sulfonate (SDBS) concentration is 20 mmol/L.
In the figure 1, crude enzyme solutions from pleurotus eryngii, coprinus comatus, AS bacteria, AC bacteria, russula vinosa, sweet lucid ganoderma and oyster mushroom are sequentially arranged from left to right, and the results show that the sweet lucid ganoderma becomes dark green after the reaction is finished, the color is the deepest, and the better aniline polymerization effect is proved.
FIG. 2 is a full-wavelength scan of aniline polymerization reaction solution catalyzed by different laccases,
the above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (4)
1. A method for preparing polyaniline by laccase catalysis is characterized in that: the laccase is from Ganoderma.
2. The method of claim 1, wherein: the laccase is derived from sweet Ganoderma of Ganoderma.
3. The method of claim 2, wherein: the specific method comprises the following steps: adding aniline, sodium dodecyl benzene sulfonate and crude enzyme solution of sweet lucid ganoderma containing laccase into the aqueous solution, then adding acetic acid buffer solution to adjust the pH value of the system to 3.0 to form a reaction system, and then standing at room temperature for reaction to obtain the ganoderma lucidum polysaccharide.
4. The method of claim 3, wherein: adding 0.3-0.5mL of crude enzyme solution of sweet lucid ganoderma containing laccase, 10mmol/L aniline concentration and 20 mmol/L sodium dodecyl benzene sulfonate into each 4mL reaction system.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911071007.1A CN110699395A (en) | 2019-11-05 | 2019-11-05 | Method for preparing polyaniline by laccase catalysis |
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| CN201911071007.1A CN110699395A (en) | 2019-11-05 | 2019-11-05 | Method for preparing polyaniline by laccase catalysis |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111321174A (en) * | 2020-04-30 | 2020-06-23 | 福州大学 | Application of laccase in catalyzing polymerization of methacrylate monomer |
| CN113322075A (en) * | 2019-06-03 | 2021-08-31 | 张琦 | Composite biological soil water-retaining agent |
Citations (6)
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|---|---|---|---|---|
| JP2001069973A (en) * | 1999-09-03 | 2001-03-21 | Daiwa Kasei Kk | Alkali laccase and its production |
| CN101638621A (en) * | 2008-09-23 | 2010-02-03 | 广州大学 | Ganoderma strain capable of producing laccase |
| CN101928699A (en) * | 2010-04-20 | 2010-12-29 | 山东省农业科学院土壤肥料研究所 | Fermentation process for improving yield of lucid ganoderma laccase |
| CN103122337A (en) * | 2013-02-05 | 2013-05-29 | 安徽大学 | Method for producing laccase by fermentation of ganoderma lucidum |
| US20150203825A1 (en) * | 2012-05-21 | 2015-07-23 | Niolas Mano | Laccase of Podospora Anserina and Uses of Same |
| CN105244510A (en) * | 2015-10-29 | 2016-01-13 | 南京理工大学 | Laccase catalyzed electrochemical assembly polyaniline / graphene oxide composite modified electrode and preparation method and application thereof |
-
2019
- 2019-11-05 CN CN201911071007.1A patent/CN110699395A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001069973A (en) * | 1999-09-03 | 2001-03-21 | Daiwa Kasei Kk | Alkali laccase and its production |
| CN101638621A (en) * | 2008-09-23 | 2010-02-03 | 广州大学 | Ganoderma strain capable of producing laccase |
| CN101928699A (en) * | 2010-04-20 | 2010-12-29 | 山东省农业科学院土壤肥料研究所 | Fermentation process for improving yield of lucid ganoderma laccase |
| US20150203825A1 (en) * | 2012-05-21 | 2015-07-23 | Niolas Mano | Laccase of Podospora Anserina and Uses of Same |
| CN103122337A (en) * | 2013-02-05 | 2013-05-29 | 安徽大学 | Method for producing laccase by fermentation of ganoderma lucidum |
| CN105244510A (en) * | 2015-10-29 | 2016-01-13 | 南京理工大学 | Laccase catalyzed electrochemical assembly polyaniline / graphene oxide composite modified electrode and preparation method and application thereof |
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| 刘禹等: ""灵芝属不同菌种漆酶活性的比较"", 《中药材》 * |
| 张亚: ""酶促苯胺类单体聚合及应用"", 《中国博士学位论文全文数据库工程科技Ⅰ辑》 * |
| 陶君等: ""灵芝漆酶活性的测定及其产漆酶条件的优化"", 《食品科学》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113322075A (en) * | 2019-06-03 | 2021-08-31 | 张琦 | Composite biological soil water-retaining agent |
| CN111321174A (en) * | 2020-04-30 | 2020-06-23 | 福州大学 | Application of laccase in catalyzing polymerization of methacrylate monomer |
| CN111321174B (en) * | 2020-04-30 | 2022-08-30 | 福州大学 | Application of laccase in catalyzing polymerization of methacrylate monomer |
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