CN110699348B - Phosphorus-containing microbial capsule and preparation method and application thereof - Google Patents
Phosphorus-containing microbial capsule and preparation method and application thereof Download PDFInfo
- Publication number
- CN110699348B CN110699348B CN201910572688.3A CN201910572688A CN110699348B CN 110699348 B CN110699348 B CN 110699348B CN 201910572688 A CN201910572688 A CN 201910572688A CN 110699348 B CN110699348 B CN 110699348B
- Authority
- CN
- China
- Prior art keywords
- phosphorus
- capsule
- phosphate
- lead
- containing compound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
Abstract
The invention discloses a phosphorus-containing microbial capsule and a preparation method and application thereof. The phosphorus-containing microbial capsule provided by the invention can improve the microbial activity, increase the contact area of phosphate-solubilizing microorganisms and phosphorus-containing materials and accelerate PO (phosphorus oxide)4 3‑The dissolution of the composite material improves the repair efficiency. The phosphorus-containing microbial capsule prepared by the invention fully exerts the synergistic repair effect of phosphorus-dissolving microbes and phosphorus-containing materials on heavy metals, shows good passivation effect on lead, can be used for stabilizing lead in soil and bottom mud, and has good application prospect.
Description
Technical Field
The invention belongs to the field of microbial stable remediation of heavy metal contaminated soil and sediment, and relates to a phosphorus-containing microbial capsule, and a preparation method and application thereof.
Background
Lead pollutes the environment, and mainly caused by lead-containing waste water and waste residue which are generated in the smelting process of lead smelting enterprises and are discharged randomly; in addition, lead-containing waste gas generated in industrial production and transportation enters the soil and sediment environment along with the settlement of the atmosphere. The problem of lead pollution of soil and bottom mud is becoming more and more serious because lead itself is difficult to degrade and is continuously accumulated in soil and bottom mud.
At present, two main approaches for treating lead pollution are provided: firstly, the lead content in soil and bottom mud is reduced by methods of soil dressing, soil changing, dredging and the like, so that the lead pollution condition is improved; and secondly, the existence form of lead in soil and bottom sediment is changed, the content of effective lead in the soil and the bottom sediment is reduced, the migration and bioavailability of the lead in the environment are reduced, and the transmission and accumulation of the lead in a food chain are reduced.
The in-situ passivation restoration method is a good choice for restoring medium and light lead polluted soil and bottom mud, meets the strategic requirements of sustainable development in China, and is widely concerned. The phosphate can reduce the toxicity and bioavailability of heavy metals in the environment to a certain extent through chemical action, and many studies at home and abroad show that phosphate materials, such as tricalcium phosphate (TCP), diammonium phosphate (DAP), Hydroxyapatite (HAP), other synthesized phosphate materials and the like can generate the almost most stable substances in the nature with lead, such as the phosphorus-lead-chloride ore and the like, so that the leaching rate of the lead in soil and bottom mud is effectively reduced. Therefore, over the last two decades, researchers have been trying to treat lead pollution using various phosphate materials as chemical stabilizers and have fully studied the mechanism of the interaction of phosphate materials with lead. Wanjia et al (university of Hunan, 2018) prepared modified nano chlorapatite and used for stable repair of lead-polluted bottom sludge, and the results show that the removal rate of toxic leaching extractable state (TCLP) lead of the bottom sludge treated by the nano chlorapatite modified by the surfactant reaches 100 percent in about 23 days. The green environmental engineering, 2017, 35(9):177-180) uses calcium dihydrogen phosphate to repair lead contaminated soil, and the result shows that the lead passivation rate can reach 69.81%.
The phosphate solubilizing microorganism can convert insoluble phosphorus into soluble phosphorus to repair heavy metal lead, and can improve the stabilizing efficiency of lead to a great extent. Metabolites generated in the microbial metabolism process and microorganisms can complex heavy metals in the environment, so that conversion among different heavy metal forms is realized, and the biological effectiveness of the heavy metals is influenced. In addition, the method for restoring the lead polluted soil and the bottom sediment by utilizing the phosphate solubilizing microorganisms does not cause great disturbance to an ecological system, can save phosphate rock resources, and is an economic and green restoration technology. The passivation effect of two strains of phosphate solubilizing bacteria on lead-polluted bottom sediment is researched by the yaoqian and the like (northwest university, 2017), the action mechanism of the phosphate solubilizing bacteria for passivating lead pollution is preliminarily discussed, and the experimental result shows that the phosphate solubilizing bacteria can obviously reduce exchangeable-state and carbonate-bound-state lead in the bottom sediment. However, the soil and the sediment have a large amount of pollutants, are complex in type and high in toxicity, and foreign microorganisms are difficult to adapt to environmental conditions in a short time, so that the growth and metabolism of the foreign microorganisms are influenced and even possibly inactivated.
In conclusion, although the phosphorus-containing material and the phosphate-solubilizing microorganism have certain effects on repairing the lead-polluted soil and the bottom mud, the phosphorus-containing material and the phosphate-solubilizing microorganism have respective disadvantages, and even if the phosphorus-containing material and the bottom mud are superposed and applied mechanically according to a conventional method, the original defects of the phosphorus-containing material and the bottom mud cannot be overcome. Therefore, there is a need to develop a novel lead passivator with good synergistic effect and simple application to effectively solve the problem of lead pollution in soil and bottom mud.
Disclosure of Invention
The invention aims to provide a phosphorus-containing microbial capsule as well as a preparation method and application thereof. The phosphorus-containing microbial capsule is simple in preparation process, can save phosphorite resources, has small influence on an ecosystem, can effectively reduce the potential risk of lead-polluted soil and bottom mud of China to the health of animals, plants and human beings, and can provide theoretical support for microbial remediation of heavy metal pollution of soil and bottom mud.
The phosphorus-containing microbial capsule provided by the invention is a liquid core capsule consisting of a capsule wall and a liquid capsule core;
the material comprising the wall comprises a hydrogel;
the material for forming the liquid capsule core comprises a phosphorus-containing compound and phosphate-solubilizing microorganisms.
In the phosphorus-containing microbial capsule, the hydrogel is selected from at least one of sodium alginate hydrogel and polyvinyl alcohol hydrogel;
the phosphorus-containing compound is selected from at least one of calcium phosphate, hydroxyapatite and calcium hydrophosphate;
the phosphate solubilizing microorganism is at least one selected from the group consisting of non-decarboxylating lecanium, bacillus thuringiensis and pseudomonas putida;
the mass ratio of the hydrogel to the phosphorus-containing compound is 1: 0.005-0.05; specifically, 1: 0.0069, 1: 0.0083, 1: 0.00964, 1: 0.011, 1: 0.012 and 1: 0.014;
the mass ratio of the hydrogel to the phosphate solubilizing microorganism is 1: 0.1-1; specifically 1: 0.12; the mass of the phosphate solubilizing microorganism is the wet weight of the thallus.
The material constituting the capsule wall may further comprise a cross-linking agent;
the cross-linking agent is selected from CaCl2At least one of an aqueous solution of (a) and an aqueous solution of boric acid;
specifically, the CaCl is2In an aqueous solution of (1), CaCl2And the amount ratio of water is 5-15 g: 100 mL; specifically, 10 g: 100 mL;
the dosage ratio of the phosphorus-containing compound to the cross-linking agent is 1g:10-500 mL; specifically, the content is 1g: 230 mL.
The method for preparing the phosphorus-containing microbial capsule comprises the following steps:
and mixing the bacteria liquid containing the phosphate-solubilizing microorganisms with a phosphorus-containing compound, and then reacting with a gelling agent in the presence of a cross-linking agent to obtain the phosphate-containing microbial capsule after the reaction is finished.
In the above method, the crosslinking agent is selected from CaCl2At least one of an aqueous solution of (a) and an aqueous solution of boric acid;
specifically, the CaCl is2In an aqueous solution of (1), CaCl2And the amount ratio of water is 5-15 g: 100 mL; specifically, 10 g: 100 mL;
the gel is selected from at least one of sodium alginate and polyvinyl alcohol hydrogel;
specifically, the sodium alginate or polyvinyl alcohol hydrogel is added into a reaction system in the form of an aqueous solution; the dosage ratio of the sodium alginate or polyvinyl alcohol hydrogel to the water is 0.2-0.5 g: 100 mL; specifically, 0.3 g: 100 mL;
the reaction with the gel is carried out by dripping the reaction product into the gel; in the dripping step, the dripping speed is 1-2 mL/min.
The bacterial liquid containing the phosphate solubilizing microorganism can be prepared according to various conventional methods, for example, the bacterial liquid can be prepared according to the following methods:
1) preparation of bacterial suspension of non-decarboxylated lechlenibacter: inoculating the cultured slant strain of the non-decarboxylated lechlenibacter into a sterile seed culture medium, and performing shake culture at 28 ℃ for 10-12 h to obtain OD600A bacterial suspension of non-decarboxylated lechlenibacter with a value between 0.8 and 1;
2) 50mL of OD600A value of 0.8And (4) centrifuging the bacterial suspension of the non-decarboxylated lechlenibacter, and discarding the supernatant to obtain a lower-layer thallus, namely the bacterial liquid containing the phosphate solubilizing microorganism.
Specifically, the mass ratio of the bacteria liquid containing the phosphate solubilizing microorganisms to the phosphorus-containing compound is 0.264:0.015 to 0.031; specifically, 0.264: 0.0217, 0.264: 0.0155, 0.264: 0.0186, 0.264: 0.0248, 0.264: 0.0279, 0.264: 0.031;
the dosage ratio of the bacteria liquid containing the phosphate solubilizing microorganisms to the cross-linking agent is 0.264: 2-10 mL; specifically, 0.264: 5 mL;
the mass ratio of the gelling agent to the phosphorus-containing compound is 1: 0.005-0.05; specifically, 1:0.01, 1: 0.0069, 1: 0.0083, 1: 0.011, 1: 0.012 or 1: 0.014;
the dosage ratio of the phosphorus-containing compound to the cross-linking agent is 1g:10-500 mL; specifically, the content is 1g: 161mL, 1g: 179mL, 1g: 202mL, 1g: 269mL, 1g: 230mL or 1g: 322 mL.
In addition, the application of the phosphorus-containing microbial capsule provided by the invention in the remediation of heavy metal contaminated soil also belongs to the protection scope of the invention. Specifically, the heavy metal is lead, cadmium or zinc;
the repair is passivation;
the dosage of the phosphorus-containing microbial capsules is 1-10% of the weight of the heavy metal contaminated soil; specifically, it was found to be 2%.
The phosphorus-containing microbial capsule provided by the invention is simple in preparation process and low in production cost, can save phosphorite resources, is easy to realize industrial scale production, has small influence on an ecosystem, can effectively reduce the potential risks of lead-polluted soil and bottom mud of China to the health of animals, plants and human beings, can provide theoretical support for microbial remediation of heavy metal pollution of soil and bottom mud, and has important application value.
Drawings
FIG. 1 is a schematic diagram of the preparation process of the microbial capsules containing phosphorus.
FIG. 2 is a picture of the appearance of the prepared microbial capsules containing phosphorus.
FIG. 3 shows the different Ca contents3(PO4)2The microbial capsule pair of (1) has Pb in liquid culture medium2+The removal effect of (1).
FIG. 4 shows the prepared phosphorus-containing microbial capsules at different initial Pb2+Effect under concentration conditions.
FIG. 5 shows the remediation effect of phosphorus-containing microbial capsules on lead-contaminated substrate sludge.
Detailed Description
The present invention will be further illustrated with reference to the following specific examples, but the present invention is not limited to the following examples. The method is a conventional method unless otherwise specified. The starting materials are commercially available from the open literature unless otherwise specified.
The strain MRP-1 used in the following examples has been deposited in China general microbiological culture Collection center (CGMCC, address: No. 3 Siro 1 Kyoh-West Luo-Yang district, Beijing) at 22.08.2017, has a deposition number of CGMCC No.14561, and is classified and named as non-decarboxylating bacterium (Leclercia adecaboxylata);
the used strain MRP-2 is preserved in China general microbiological culture Collection center (CGMCC for short, the address is No. 3 Siro No.1 of Beijing city Kogyo-Xinyang district), the preservation number is CGMCC NO.15337, and the strain is classified and named as Pseudomonas putida (Pseudomonas putida) in 2018, 02 and 02 days;
the used strain MRP-3 is preserved in China general microbiological culture Collection center (CGMCC for short, the address is No. 3 Siro No.1 of Beijing market open-south district), the preservation number is CGMCC NO.15338, and the strain is classified and named as Bacillus thuringiensis (Bacillus thuringiensis) in 2018, 02 and 02 days.
Example 1 preparation of phosphor-containing microbial capsules.
The method specifically comprises the following steps:
(1) bacterial suspension preparation of non-decarboxylated lechlenibacter: inoculating the cultured slant strain of the non-decarboxylated lechlenibacter into a sterile seed culture medium, and performing shake culture at 28 ℃ for 10-12 h to obtain OD600A bacterial suspension of non-decarboxylated lechlenibacter with a value between 0.8 and 1.
(2) 50mL of OD600Centrifuging a bacterial suspension of the non-decarboxylated lechlenibacter with the value of 0.8-1, and discarding a supernatant to obtain a lower layer of bacteria with the mass of about 0.264g (wt).
(3) Adding 0.0217g of Ca to the thalli obtained in the step (2)3(PO4)2Then, 5mL of a crosslinking agent was added and mixed well.
(4) And (3) dropwise adding the mixed suspension in the step (3) into a continuously stirred sodium alginate aqueous solution by using a 10mL syringe at the dropping speed of 1mL/min, and obtaining the phosphorus-containing microbial capsule after the dropwise adding is finished, wherein the dropping speed is shown in figure 2.
In the step (3), the cross-linking agent is prepared according to the following weight ratio of 10 g: 100mL of anhydrous CaCl2Dissolving in purified water.
The sodium alginate aqueous solution in the step (4) is 0.3 g: dissolving sodium alginate in purified water at a ratio of 100mL, and heating in water bath at 40 deg.C to dissolve. The total mass dosage of the sodium alginate is 2.17 g; i.e. sodium alginate and Ca3(PO4)2The mass ratio of (A) to (B) is 1: 0.01;
the average particle size of the microbial capsules containing phosphorus obtained in this example was 0.5 cm. In the phosphorus-containing microbial capsule, the capsule wall is sodium alginate hydrogel, and the capsule core is composed of non-decarboxylated Lerlachella thallus and Ca3(PO4)2And (4) forming. Wherein, in each capsule, 1g of Ca corresponding to the sodium alginate hydrogel3(PO4)2The mass of (2) was 0.00964g, and the corresponding cell mass (wet weight) was 0.11733 g.
Example 2 phosphorus content in microbial capsules containing phosphorus vs. Pb2+The effect of performance is removed.
The method specifically comprises the following steps:
(1) the Ca obtained in step (3) of example 1 was prepared in the same manner as in example 13(PO4)2The mass of the microbial capsules is replaced by 0.0155g, 0.0186g, 0.0248g, 0.0279g and 0.031g in sequence, and the phosphorus-containing microbial capsules with the average particle size of 0.5cm are obtained. In the above phosphorus-containing microbial capsule, the capsule wall is sodium alginate hydrogel, and the capsule core is composed of non-decarboxylated Leillus and Ca3(PO4)2The liquid capsule core is formed. Wherein, in each capsule, 1g of Ca corresponding to the sodium alginate hydrogel3(PO4)2The mass of (a) was 0.0069, 0.0083, 0.011, 0.012 and 0.014g in this order, and the corresponding cell bodies (wet weight) were all 0.11733 g.
(2) The prepared phosphorus-containing microbial capsules are added into a sterile lead-containing inorganic salt culture medium according to the adding amount of 2 percent.
(3) And (3) then, culturing the culture medium added with the phosphorus-containing microbial capsules in the step (2) for 24 hours in a shaking incubator at 28 ℃ and 120 rpm.
(4) For residual Pb in the culture medium2+The ions were measured and analyzed by ICP-MS (inductively coupled plasma mass spectrometry), and the measurement results are shown in FIG. 3.
The inorganic salt culture medium in the step (2) comprises the following main components: glucose 10.0g/L, NaCl 0.3.3 g/L, (NH)4)2SO40.5g/L、MgSO4·7H2O 0.3g/L、MnSO4·4H2O 0.03g/L、KCl 0.3g/L、FeSO4·7H2O0.03g/L. Adding 1mM Pb2+(by Pb (NO)3)2Provided), the medium was sterilized at 121 ℃ under 110kPa for 30 min.
FIG. 3 shows different Ca3(PO4)2Content of Pb in culture medium of microbial capsule pair2+The removal rate of (3). As can be seen from FIG. 3, Pb increased with the phosphorus content2+The removal rate is increased continuously when Ca is added3(PO4)2When the content is 0.07mM, the removal rate reaches 98 percent, and Ca is continuously increased3(PO4)2The removal rate does not vary much. Thus, when encapsulating 0.07mM Ca3(PO4)2Then, the best phosphorus-containing microbial capsule can be obtained.
Example 3 phosphorous-containing microbial capsules removal performance for different levels of lead.
The method specifically comprises the following steps:
(1) the same procedure as in example 1 was used to prepare the microbial capsules containing phosphorus.
(2) Adding the phosphorus-containing microbial capsules prepared in the step (1) into sterile improved inorganic salt culture media containing lead with different concentrations according to the addition of 2%.
(3) And (3) then, culturing the culture medium added with the phosphorus-containing microbial capsules in the step (2) for 24 hours in a shaking incubator at 28 ℃ and 120 rpm.
(4) For residual Pb in the culture medium2+The measurement of ions was performed by the same method as in example 2, and the measurement results are shown in FIG. 4.
The inorganic salt medium used in step (2) contains the same main components as in example 2, wherein Pb is contained2+The content of (b) is 0.02mM, 0.05mM, 1mM, 2.5mM, 3mM, respectively.
FIG. 4 shows Pb in medium with microbial capsules containing phosphorus versus initial lead concentration2+The removal effect of (1). As can be seen from the graph, the phosphorus-containing microbial capsules were aligned to Pb at an initial lead concentration of 1mM or less2+The removal rate of the catalyst can reach about 96 percent when being Pb2+At concentrations above 1mM, the capsules are aligned to Pb2+The removal rate of (2) is greatly reduced to about 40%, which is probably due to Pb2+The concentration is too high, the toxic effect on the microorganisms is increased, the growth and metabolism of the microorganisms are inhibited, and thus the lead-free antibacterial agent has the effect on Pb2+The removal rate of (2) is decreased. From this, we can conclude that the phosphorus-containing microbial capsules are suitable for Pb of 1mM or less2+Environmental conditions of concentration.
Example 4 simulated remediation of lead contaminated substrate sludge by phosphorus-containing microbial capsules
The method specifically comprises the following steps:
(1) collecting and treating bottom mud: selecting a river sampling area with slow water flow, a distance from a sewage discharge outlet and stable sediment, sampling around the river by adopting a checkerboard-type point distribution method, wherein the sampling depth is 0-20cm, collecting 10 bags of samples, filling the samples into plastic bags, and transporting the plastic bags back to a laboratory. Spreading the bottom mud at different points in shade, air drying for 3-5 days, removing impurities such as large gravel and plant debris, and mixing. Grinding the air-dried bottom mud, passing through a nylon mesh screen with the diameter of 1mm, and measuring the basic physicochemical property of the bottom mud; the rest air-dried bottom mud contains 500mg/L Pb2+The lead nitrate solution is artificially contaminated to stabilize it for later use.
(2) Weighing 4g of the stable air-dried sediment into a 150mL conical flask, adding 40mL of inorganic salt culture medium, and sterilizing at 121 ℃ and 110kPa for 30 min.
(3) The same procedure as in example 1 was used to prepare the microbial capsules containing phosphorus.
(4) And (3) adding the phosphorus-containing microbial capsules prepared in the step (3) into the simulated lead polluted bottom mud in the step (2) according to the addition of 2%.
(5) Then, the muddy water mixture added with the phosphorus-containing microbial capsules in the step (4) is cultured in a shaking incubator at 28 ℃ and 120rpm for 10 days, samples are taken at 0d, 3d, 5d and 10d respectively, and the existence form of lead in the substrate sludge is analyzed, and the determination result is shown in FIG. 5.
The inorganic salt culture medium in the step (2) comprises the following main components: glucose 10.0g/L, NaCl 0.3.3 g/L, (NH)4)2SO40.5g/L、MgSO4·7H2O 0.3g/L、MnSO4·4H2O 0.03g/L、KCl 0.3g/L、FeSO4·7H2O0.03g/L。
FIG. 5 shows the change of lead content in different forms in the process of simulating and repairing lead polluted bottom mud by using the phosphorus-containing microbial capsule. As can be seen from the figure, the content of the weak acid lead in the sediment is continuously reduced along with the prolonging of the time, after 10 days of restoration, the content of the weak acid lead is reduced to 14 percent from the initial 28 percent, and the proportion of the lead in the reducible state, the oxidizable state and the residue state is continuously increased, which shows that the weak acid lead is continuously converted to other more stable forms, so that the phosphorus-containing microbial capsule has a better stabilizing effect on the lead-polluted sediment.
Claims (8)
1. A phosphorus-containing microbial capsule is a liquid core capsule consisting of a capsule wall and a liquid capsule core;
the material comprising the wall comprises a hydrogel;
the material for forming the liquid capsule core comprises a phosphorus-containing compound and phosphate-solubilizing microorganisms;
the hydrogel is selected from at least one of sodium alginate hydrogel and polyvinyl alcohol hydrogel;
the phosphorus-containing compound is calcium phosphate;
the phosphate solubilizing microorganism is at least one selected from the group consisting of non-decarboxylating lecanium, bacillus thuringiensis and pseudomonas putida;
the mass ratio of the hydrogel to the phosphorus-containing compound is 1: 0.005-0.05;
the mass ratio of the hydrogel to the phosphate solubilizing microorganism is 1: 0.1-1;
the material forming the capsule wall also comprises a cross-linking agent;
the cross-linking agent is CaCl2An aqueous solution of (a);
the dosage ratio of the phosphorus-containing compound to the cross-linking agent is 1g:10-500 mL;
the preparation method of the phosphorus-containing microbial capsule comprises the following steps: and mixing the bacteria liquid containing the phosphate-solubilizing microorganisms with a phosphorus-containing compound, and then reacting with a gelling agent in the presence of a cross-linking agent to obtain the phosphate-containing microbial capsule after the reaction is finished.
2. A method of making the phosphorus-containing microbial capsule of claim 1, comprising:
and mixing the bacteria liquid containing the phosphate-solubilizing microorganisms with a phosphorus-containing compound, and then reacting with a gelling agent in the presence of a cross-linking agent to obtain the phosphate-containing microbial capsule after the reaction is finished.
3. The method of claim 2, wherein: the reaction with the gelling agent is carried out by dripping into the gelling agent.
4. The method of claim 3, wherein: in the dripping step, the dripping speed is 1-2 mL/min.
5. The method according to any one of claims 2-4, wherein: the mass ratio of the bacteria liquid containing the phosphate solubilizing microorganisms to the phosphorus-containing compound is 0.264: 0.015-0.031;
the dosage ratio of the bacterial liquid containing the phosphate solubilizing microorganisms to the cross-linking agent is 0.264g to 2-10 mL;
the mass ratio of the gel to the phosphorus-containing compound is 1: 0.005-0.05;
the dosage ratio of the phosphorus-containing compound to the cross-linking agent is 1g:10-500 mL.
6. The phosphorus-containing microbial capsule of claim 1, wherein the capsule is used for repairing heavy metal contaminated soil.
7. Use according to claim 6, characterized in that: the heavy metal is lead, cadmium or zinc;
the repair is passivation;
the dosage of the phosphorus-containing microbial capsules is 1-10% of the weight of the heavy metal contaminated soil.
8. Use according to claim 7, characterized in that: the dosage of the phosphorus-containing microbial capsules is 2 percent of the weight of the heavy metal contaminated soil.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910572688.3A CN110699348B (en) | 2019-06-28 | 2019-06-28 | Phosphorus-containing microbial capsule and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910572688.3A CN110699348B (en) | 2019-06-28 | 2019-06-28 | Phosphorus-containing microbial capsule and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110699348A CN110699348A (en) | 2020-01-17 |
| CN110699348B true CN110699348B (en) | 2021-08-17 |
Family
ID=69193053
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910572688.3A Active CN110699348B (en) | 2019-06-28 | 2019-06-28 | Phosphorus-containing microbial capsule and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110699348B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111394101B (en) * | 2020-03-13 | 2021-04-27 | 江苏和合环保集团有限公司 | Preparation method of biomass long-acting heavy metal passivator |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105018458A (en) * | 2015-07-31 | 2015-11-04 | 湖南大学 | Compound microbial agent as well as preparation method and application thereof |
| CN105315998A (en) * | 2015-10-14 | 2016-02-10 | 济南益邦生物科技有限公司 | Passivating agent for reducing activity of Pb and Cr in alkaline soil and application thereof |
| CN105598158A (en) * | 2016-01-28 | 2016-05-25 | 中南大学 | Phosphorus-based biochar material, and preparation and application thereof |
| CN105647539A (en) * | 2016-01-28 | 2016-06-08 | 中南大学 | Application of lead-contaminated soil restoring fixing agent synthesized based on microorganism assembly |
| CN107557349A (en) * | 2017-10-11 | 2018-01-09 | 华中农业大学 | A kind of method that high vigor phosphate solubilizing bacteria solid fungicide is made using bagasse as carrier |
| CN107746711A (en) * | 2017-10-18 | 2018-03-02 | 河北大学 | A kind of soil heavy metal cadmium passivator and preparation method and application |
| CN107841472A (en) * | 2017-11-10 | 2018-03-27 | 北京林业大学 | A kind of Leclercia adecarboxylata and its application with dissolving P capacity |
| CN108672490A (en) * | 2018-05-17 | 2018-10-19 | 南京工业大学 | A kind of microorganism passivating method for remediating heavy metal lead-contaminated soil |
| CN108752138A (en) * | 2018-07-25 | 2018-11-06 | 美丽国土（北京）生态环境工程技术研究院有限公司 | The complex repairation agent administered for heavy metals in farmland cadmium pollution and restorative procedure and application |
| CN109926435A (en) * | 2019-04-11 | 2019-06-25 | 北京净界新宇环保科技有限公司 | A kind of processing method of heavy metal cadmium |
| CN110064647A (en) * | 2019-06-05 | 2019-07-30 | 湖南双晟科技信息咨询有限公司 | A kind of processing method of cadmium pollution soil |
| CN110586642A (en) * | 2019-09-10 | 2019-12-20 | 铜仁市万山区植保植检站 | Method for repairing lead-cadmium contaminated soil |
-
2019
- 2019-06-28 CN CN201910572688.3A patent/CN110699348B/en active Active
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105018458A (en) * | 2015-07-31 | 2015-11-04 | 湖南大学 | Compound microbial agent as well as preparation method and application thereof |
| CN105315998A (en) * | 2015-10-14 | 2016-02-10 | 济南益邦生物科技有限公司 | Passivating agent for reducing activity of Pb and Cr in alkaline soil and application thereof |
| CN105598158A (en) * | 2016-01-28 | 2016-05-25 | 中南大学 | Phosphorus-based biochar material, and preparation and application thereof |
| CN105647539A (en) * | 2016-01-28 | 2016-06-08 | 中南大学 | Application of lead-contaminated soil restoring fixing agent synthesized based on microorganism assembly |
| CN107557349A (en) * | 2017-10-11 | 2018-01-09 | 华中农业大学 | A kind of method that high vigor phosphate solubilizing bacteria solid fungicide is made using bagasse as carrier |
| CN107746711A (en) * | 2017-10-18 | 2018-03-02 | 河北大学 | A kind of soil heavy metal cadmium passivator and preparation method and application |
| CN107841472A (en) * | 2017-11-10 | 2018-03-27 | 北京林业大学 | A kind of Leclercia adecarboxylata and its application with dissolving P capacity |
| CN108672490A (en) * | 2018-05-17 | 2018-10-19 | 南京工业大学 | A kind of microorganism passivating method for remediating heavy metal lead-contaminated soil |
| CN108752138A (en) * | 2018-07-25 | 2018-11-06 | 美丽国土（北京）生态环境工程技术研究院有限公司 | The complex repairation agent administered for heavy metals in farmland cadmium pollution and restorative procedure and application |
| CN109926435A (en) * | 2019-04-11 | 2019-06-25 | 北京净界新宇环保科技有限公司 | A kind of processing method of heavy metal cadmium |
| CN110064647A (en) * | 2019-06-05 | 2019-07-30 | 湖南双晟科技信息咨询有限公司 | A kind of processing method of cadmium pollution soil |
| CN110586642A (en) * | 2019-09-10 | 2019-12-20 | 铜仁市万山区植保植检站 | Method for repairing lead-cadmium contaminated soil |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110699348A (en) | 2020-01-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Eroglu et al. | Nitrate removal from liquid effluents using microalgae immobilized on chitosan nanofiber mats | |
| Ledin | Accumulation of metals by microorganisms—processes and importance for soil systems | |
| CN107488620B (en) | Cadmium-polluted rice field soil microbial passivator | |
| Liu et al. | Enhanced phytoextraction of heavy metals from contaminated soil by plant co-cropping associated with PGPR | |
| US10457612B1 (en) | Slag bacterial fertilizer and preparation method thereof and method for improving degraded soil | |
| Parameswari et al. | Biosorption and metal tolerance potential of filamentous fungi isolated from metal polluted ecosystem. | |
| CN112474781B (en) | Composite material for repairing heavy metal contaminated soil and preparation method and application thereof | |
| CN110586642B (en) | Method for repairing lead-cadmium contaminated soil | |
| CN111748351B (en) | Conditioner for repairing heavy metal contaminated soil and preparation method and application thereof | |
| Sameera et al. | Role of biosorption in environmental cleanup | |
| CA3035337A1 (en) | Bioremediation of heavy metal contaminated geomaterials by indigenous microorganisms | |
| CN111849501B (en) | Soil conditioner for repairing soil nickel pollution and preparation method and use method thereof | |
| CN105013815A (en) | Biological remediation method for polycyclic aromatic hydrocarbon and heavy metal compound contaminated soil | |
| JP2013068556A (en) | Method for purifying environment contaminated by radioactive cesium | |
| CN110699348B (en) | Phosphorus-containing microbial capsule and preparation method and application thereof | |
| CN110373199B (en) | Composite soil repairing agent and preparation method and repairing method thereof | |
| Hamzah et al. | The influence of rice husk and tobacco waste biochars on soil quality | |
| CN109702007B (en) | In-situ remediation method for moderate farmland heavy metal cadmium pollution | |
| CN110724535A (en) | Novel ecological garden greening soil remediation treatment agent and preparation method thereof | |
| CN106630510B (en) | Modified Nano chlorapatite and preparation method thereof | |
| CN105647539A (en) | Application of lead-contaminated soil restoring fixing agent synthesized based on microorganism assembly | |
| CN111849817B (en) | Phosphate-dissolving bacteria synergistic iron-based phosphorus-containing nano material system and application thereof in passivation of heavy metals | |
| JP2002507114A (en) | Methods for reducing lead bioavailability by lead sequestered soil bacteria. | |
| Stefanescu et al. | Bioremediation perspective of Bacillus megaterium towards heavy metals in environments enriched with phosphogypsum | |
| CN110066658B (en) | Passivator for mercury contaminated soil and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |