CN110693832A - Cinnamomum camphora extract microemulsion gel and preparation method thereof - Google Patents
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Abstract
The invention discloses camphor extract micro-emulsion gel and a preparation method thereof. The preparation method comprises the following steps: the preparation method of the cinnamomum camphora extract microemulsion comprises the following steps: weighing the camphor extract, adding the camphor extract into the oil phase, then adding the emulsifier and the co-emulsifier, and stirring uniformly under a heating condition to obtain an oily mixture; adding water into the oily mixture under stirring for emulsification to obtain Cinnamomum camphora extract microemulsion; the preparation steps of the gel matrix are as follows: weighing carbomer-940, adding glycerol for wetting, adding water, stirring, and standing to swell completely to obtain gel matrix; mixing the cinnamomum camphora extract micro emulsion and a gel matrix: adding the cinnamomum camphora extract micro emulsion into a gel matrix, uniformly stirring, then adjusting the pH value, and centrifuging to obtain the cinnamomum camphora extract micro emulsion gel. The cinnamomum camphora extract micro-emulsion gel has excellent performance, and has obvious anti-inflammatory activity and bacteriostatic action.
Description
Technical Field
The invention relates to the technical field of medicinal preparations, in particular to camphor extract micro-emulsion gel and a preparation method thereof.
Background
Cinnamomum camphora (L.) Presl, also known as Cinnamomum camphora, camphorwood, sassafras, etc., is a evergreen plant widely distributed in subtropical areas such as Guangdong, Guangxi, Hunan, etc. The cinnamomum camphora has unique feeling to cinnamomum camphora from old times, is derived from special fragrance of the cinnamomum camphora, has certain medicinal value, is a traditional Chinese medicinal material in China, is prepared from roots, branches and leaves and the like, and has the effects of resisting thrombus, preventing arteriosclerosis, resisting tumors, resisting oxidative aging, rheumatism, sprain, bronchitis, asthma and the like.
The research shows that the volatile essential oil of the leaves mainly comprises 5 chemical types. Wherein the leaf contains 1.53 to 1.93 percent of borneol type essential oil, and the chemical main component of the leaf is dextroborneol (81.78 percent). Because the branches and leaves of the cinnamomum camphora contain rich d-borneol, the cinnamomum camphora gradually becomes one of the important raw materials for obtaining d-borneol. D-borneol (also called natural borneol), which belongs to monoterpene alcohol in terpenoids, and the molecular formula is C10H18O, which is a valuable medical material, high-grade aromatic flavor, chemical raw material, has been used in food and folk medicine in china and india. It can be used externally or orally, and can clear away heat and toxic material,the oral administration can achieve the effects of inducing resuscitation with aromatics and refreshing brain, and is often used as an adjuvant drug of various compound Chinese medicinal preparations. Many traditional Chinese medicines, such as YUNNANBAIYAO, SHUANGKUIFENG powder, FUFANGDANSHEN dripping pill, ANGONGNIUHUANG pill, etc., contain natural Borneolum Syntheticum. Meanwhile, the natural borneol is used as a high-grade spice, has strong fragrance, is fresh and sharp, is exciting, and is called as plant musk. However, in daily application, people use the borneol as an adjuvant drug, and rarely use the borneol as a main drug to study the percutaneous permeation effect of the borneol in different dosage forms, so that the natural borneol has certain curative effect on certain diseases.
With the large-scale application and development of oral medicines, people find that many oral medicines stimulate gastrointestinal tracts, and adverse reactions caused by the oral medicines are more obvious as the medication time is prolonged, so that the sight of people is gradually transferred to an external administration mode and a preparation thereof, such as an external transdermal administration mode. Compared with the traditional oral administration, the transdermal administration mode avoids the possible first pass effect of the liver and the inactivation of gastrointestinal digestive juice to the medicament on one hand, thereby improving the bioavailability of the medicament, reducing the stimulation of the medicament to the gastrointestinal tract, obtaining better treatment effect, simultaneously ensuring that the medicament can continuously enter the body at a constant speed within a longer time, reducing the administration times and prolonging the administration interval; the medicine can enter the body at a specified rate, so that stable and effective blood concentration is maintained, the peak valley phenomenon of the blood concentration caused by an oral administration mode is avoided, and the occurrence of adverse reaction is further reduced. On the other hand, the use is convenient, the patient can independently use the medicine, and if any discomfort is found, the medicine administration can be interrupted at any time; particularly, as a delivery path of the drug of the biological macromolecule, the drug can effectively solve the problems of volatile activity and short half-life period of the biological macromolecule in the digestive tract. Therefore, research and development of transdermal absorption preparations are receiving much attention. However, transdermal drug delivery is limited in that its absorption area is small, a certain transdermal absorption rate must be secured to ensure the therapeutic effect of the drug, and conventional ointments, gels and other dosage forms have not been satisfactory, and thus, the barrier function of the skin and mucous membranes is overcome and the transdermal permeation of the drug is promoted for a prescribed time, and it is generally required to search for and study a new dosage form for transdermal drug absorption.
Microemulsion gels (MBGs) are a new formulation, which has been gradually developed over the past decade, and are microemulsions prepared from an oil phase, a surfactant, a co-surfactant, and an aqueous phase, and form a transparent, uniform, stable gel network structure containing microemulsion droplets in a gel matrix made of a medicinal polymer material such as a natural polymer, a cellulose derivative, and a block polymer. The microemulsion can increase the solubility and transdermal absorption of the drug, improve the stability of the drug and prolong the action time of the drug. However, microemulsions are inherently liquid, highly fluid, and poorly adhere as a transdermal delivery vehicle. According to the good adhesiveness of the gel, the microemulsion is further prepared into microemulsion gel, and the problems of poor adhesiveness of a microemulsion carrier, poor smearing property on skin and short retention time of the skin can be solved. The microemulsion gel not only inherits the functions of the microemulsion in solubilizing, reducing the diffusion barrier of the skin and increasing the transdermal permeability of the medicament, but also improves the viscosity of the microemulsion, the adhesiveness with the skin and the spreadability, and because the viscosity of the system is increased, the diffusion of the medicament in MBGs is hindered, the effective diffusion coefficient is reduced, and the medicinal effect of the medicament can be kept for a longer time. Compared with the conventional microemulsion, the microemulsion gel is more suitable to be used as a carrier for transdermal drug delivery.
However, at present, no micro-emulsion gel preparation taking camphor volatile oil extract (the main component is natural borneol) as a main drug is available. Therefore, the development of the cinnamomum camphora volatile oil extract micro-emulsion gel preparation, particularly the development of the cinnamomum camphora volatile oil extract micro-emulsion gel preparation with excellent performance, has important significance. However, the cinnamomum camphora volatile oil extract micro-emulsion gel preparation with excellent performance has to have higher scores on the scores of performance indexes such as appearance, viscosity, greasiness, spreadability, moisturizing rate and the like.
Disclosure of Invention
The technical problem to be solved is to provide a preparation method of camphor extract micro-emulsion gel. The camphor extract micro-emulsion gel prepared by the method of the invention has higher scores on the scores of performance indexes such as appearance, viscosity, greasiness, spreadability, moisture retention rate and the like.
The technical problem to be solved by the invention is realized by the following technical scheme:
a preparation method of camphor extract microemulsion gel comprises the following steps:
the preparation method of the cinnamomum camphora extract microemulsion comprises the following steps: weighing the camphor extract, adding the camphor extract into the oil phase, then adding the emulsifier and the co-emulsifier, and stirring uniformly under a heating condition to obtain an oily mixture; adding water into the oily mixture under stirring for emulsification to obtain Cinnamomum camphora extract microemulsion;
the preparation steps of the gel matrix are as follows: weighing carbomer-940, adding glycerol for wetting, adding water, stirring, and standing to swell completely to obtain gel matrix;
mixing the cinnamomum camphora extract micro emulsion and a gel matrix: adding the cinnamomum camphora extract micro emulsion into a gel matrix, uniformly stirring, adjusting the pH value to 5-6, and centrifuging to obtain the cinnamomum camphora extract micro emulsion gel.
Preferably, the oil phase in the preparation step of the cinnamomum camphora extract microemulsion is castor oil.
Preferably, the emulsifier in the preparation step of the cinnamomum camphora extract microemulsion is polyoxyethylene castor oil.
Preferably, the coemulsifier in the preparation step of the cinnamomum camphora extract microemulsion is 1, 2-propylene glycol.
The research of the inventor finds that in the preparation process of the cinnamomum camphora extract microemulsion, the selection of specific types of the oil phase, the emulsifier and the co-emulsifier plays a role in determining the scores of performance indexes such as the appearance property, the viscosity, the greasiness, the spreadability and the moisturizing rate of the cinnamomum camphora extract microemulsion gel; through a large number of experiments, the inventor finds that the micro-emulsion gel of the camphor extract obtained subsequently can be guaranteed to have higher scores on performance indexes such as appearance, viscosity, greasiness, spreadability, moisture retention rate and the like only under a micro-emulsion system consisting of castor oil as an oil phase, polyoxyethylene castor oil as an emulsifier and 1, 2-propylene glycol as an auxiliary emulsifier. The oil phase, the emulsifier and the co-emulsifier are an integral system, and improper selection of any component in the system can result in that the camphor extract micro-emulsion gel obtained by subsequent preparation cannot obtain better performance score.
Preferably, in the preparation step of the cinnamomum camphora extract microemulsion, the mass ratio of the cinnamomum camphora extract to the oil phase to the emulsifier to the co-emulsifier is 0.25-0.35: 0.4-0.6: 1.1-1.4: 0.2 to 0.3.
Most preferably, in the preparation step of the cinnamomum camphora extract microemulsion, the mass ratio of the cinnamomum camphora extract to the oil phase to the emulsifier to the co-emulsifier is 0.3: 0.5: 1.25: 0.25.
the inventor further researches and discovers that in a micro-emulsion system consisting of castor oil serving as an oil phase, polyoxyethylene castor oil serving as an emulsifier and 1, 2-propylene glycol serving as a co-emulsifier, the dosage ratio of the micro-emulsion system to the cinnamomum camphora extract and the dosage ratio of the oil phase, the emulsifier and the co-emulsifier in the system also have important influence on the performance of the micro-emulsion gel of the cinnamomum camphora extract prepared subsequently; the inventor finds out through a large number of experiments that the camphor extract micro-emulsion gel obtained by the subsequent preparation can be guaranteed to have higher scores on performance indexes such as appearance, viscosity, greasiness, spreadability, moisture retention rate and the like only under the condition of the mass and dosage ratio of the camphor extract, the oil phase, the emulsifier and the co-emulsifier.
Preferably, in the preparation step of the gel matrix, the mass ratio of carbomer-940 to glycerin wetting to water is 0.08-0.09: 0.5-0.6: 10.0 to 11.0.
The inventor researches and discovers that the dosage ratio of the components in the gel matrix also has influence on the performance of the camphor extract micro-emulsion gel obtained in the subsequent preparation. The above-mentioned ratio of the amounts of the respective ingredients in the gel base is the optimum ratio found by the inventors.
Preferably, the mass and dosage ratio of the camphor extract microemulsion to the gel matrix in the step of mixing the camphor extract microemulsion with the gel matrix is 3-4: 6 to 7.
Preferably, the mass ratio of the cinnamomum camphora extract microemulsion to the gel matrix in the step of mixing the cinnamomum camphora extract microemulsion and the gel matrix is 4: 6.
the inventor researches and discovers that the dosage ratio of the cinnamomum camphora extract micro-emulsion to the gel matrix has important influence on the performance of the cinnamomum camphora extract micro-emulsion gel prepared subsequently. The above ratio of the amount of the micro emulsion of the cinnamomum camphora extract to the amount of the gel base is the optimum ratio found by the inventors.
The cinnamomum camphora extract refers to volatile oil components extracted from cinnamomum camphora leaves; specifically, the volatile oil component may be distilled by a conventional steam distillation method.
The camphor extract can be prepared by a method comprising the following steps: weighing 150g of camphor leaves, adding 1-2L of water, extracting by using a volatile oil extraction device to obtain an oil-water mixture, and then adding ethyl acetate into the oil-water mixture for extraction; separating an ethyl acetate layer, adding excessive anhydrous sodium sulfate, fully stirring and shaking, standing for 10-30 min, and absorbing residual water in the solvent; and finally, filtering, concentrating the filtrate, and removing ethyl acetate to obtain the camphor wood extract.
The invention also provides the cinnamomum camphora extract micro-emulsion gel prepared by the preparation method.
Has the advantages that: the invention provides a brand-new preparation method of camphor extract micro-emulsion gel, which has excellent performance and must have higher scores on the scores of performance indexes such as appearance, viscosity, greasiness, spreadability, moisture retention rate and the like. In addition, experiments show that the cinnamomum camphora extract micro-emulsion gel prepared by the method has a certain antibacterial effect on staphylococcus aureus, surface staphylococcus and escherichia coli; the camphor extract micro-emulsion gel can obviously inhibit the swelling of the feet of the mice caused by fresh egg white, and has better effect than a positive control drug dexamethasone, which shows that the camphor extract micro-emulsion gel has obvious anti-inflammatory activity and bacteriostatic action.
Drawings
Fig. 1 is a graph showing the influence of the cinnamomum camphora extract microemulsion gel on egg white-induced foot swelling.
Detailed Description
The present invention is further explained below with reference to specific examples, which are not intended to limit the present invention in any way.
The performance scoring standards of the camphor extract microemulsion gel prepared by the invention are shown in table 1:
TABLE 1 Cinnamomum camphora extract microemulsion gel index grading Standard
The moisture retention in table 1 was measured by the following method: taking a proper amount of cinnamomum camphora extract microemulsion gel, precisely weighing, putting into a dry and clean weighing bottle, recording the amount (m1) of the added microemulsion gel and the mass (m0) of the bottle, and putting the weighing bottle into a constant-temperature and constant-humidity sealed dryer, namely, a room temperature (25 +/-2) DEG C and a Relative Humidity (RH) of 40 percent (saturated potassium carbonate solution). After 24h of standing, the samples were taken out, precision weighed, respectively, and the mass of the sample was recorded (m2), according to the formula: the moisture retention rate was m2/(m1-m0), and the moisture retention rate of the sample was calculated.
The cinnamomum camphora extracts in the following examples are all prepared by the following method: weighing 150g of cinnamomum camphora leaves, adding 1.5L of water, extracting by using a volatile oil extraction device to obtain an oil-water mixture, and then adding ethyl acetate into the oil-water mixture for extraction; separating the ethyl acetate layer, adding excessive anhydrous sodium sulfate, stirring, shaking, standing for 20min to absorb residual water in the solvent; and finally, filtering, concentrating the filtrate, and removing ethyl acetate to obtain the camphor wood extract.
Example 1 preparation of Cinnamomum camphora extract microemulsion gel
(1) Weighing 0.3g of cinnamomum camphora extract, adding the cinnamomum camphora extract into 0.5g of castor oil, adding 1.25g of polyoxyethylene castor oil (EL) and 0.25g of 1, 2-propylene glycol, heating in a water bath at 30 ℃ until the materials are completely dissolved, uniformly stirring to obtain an oily mixture, slowly dripping 5mL of distilled water into the oily mixture under the constant-temperature magnetic stirring at 30 ℃ for emulsification to obtain cinnamomum camphora extract microemulsion;
(2) weighing 0.084g of carbomer-940, adding 0.525g of glycerol for wetting, adding distilled water to 10.8g, stirring uniformly, and standing for 24h to fully swell to obtain a gel matrix;
(3) slowly adding the cinnamomum camphora extract micro emulsion into a gel matrix (wherein the mass ratio of the cinnamomum camphora extract micro emulsion to the gel matrix is 4: 6), uniformly stirring, dropwise adding a triethanolamine solution with the volume fraction of 20% to adjust the pH value to 5.43, and centrifuging at 4000r/min for 5min to remove bubbles to obtain the cinnamomum camphora extract micro emulsion gel.
Through evaluation, the cinnamomum camphora extract microemulsion gel prepared in the example 1 has the appearance property score of 20, the viscosity score of 19, the greasiness score of 18, the spreadability score of 20 and the moisture retention rate score of 20; the total fraction of the performance evaluation and preparation is 97 points.
Example 2 preparation of Cinnamomum camphora extract microemulsion gel
(1) Weighing 0.25g of cinnamomum camphora extract, adding the cinnamomum camphora extract into 0.4g of castor oil, adding 1.2g of polyoxyethylene castor oil (EL) and 0.3g of 1, 2-propylene glycol, heating in a water bath at 30 ℃ until the materials are completely dissolved, uniformly stirring to obtain an oily mixture, slowly dripping 5mL of distilled water into the oily mixture under the constant-temperature magnetic stirring at 30 ℃ for emulsification to obtain cinnamomum camphora extract microemulsion;
(2) weighing 0.08g of carbomer-940, adding 0.6g of glycerol for wetting, adding distilled water to 10.8g, stirring uniformly, and standing for 24h to fully swell to obtain a gel matrix;
(3) slowly adding the cinnamomum camphora extract micro emulsion into a gel matrix (wherein the mass ratio of the cinnamomum camphora extract micro emulsion to the gel matrix is 4: 6), uniformly stirring, dropwise adding a triethanolamine solution with the volume fraction of 20% to adjust the pH value to 5.43, and centrifuging at 4000r/min for 5min to remove bubbles to obtain the cinnamomum camphora extract micro emulsion gel.
Evaluation shows that the cinnamomum camphora extract microemulsion gel prepared in the example 2 has 18 points of appearance, 17 points of viscosity, 16 points of greasiness, 18 points of spreadability and 18 points of moisture retention rate; the total preparation fraction for performance evaluation was 87 points.
Example 3 preparation of Cinnamomum camphora extract microemulsion gel
(1) Weighing 0.35g of camphor extract, adding the camphor extract into 0.6g of castor oil, then adding 1.3g of polyoxyethylene castor oil (EL) and 0.2g of 1, 2-propylene glycol, heating in a water bath at 30 ℃ until the polyoxyethylene castor oil (EL) and the 1, 2-propylene glycol are completely dissolved, uniformly stirring to obtain an oily mixture, slowly dripping 5mL of distilled water into the oily mixture under the constant-temperature magnetic stirring at 30 ℃ for emulsification to obtain camphor extract microemulsion;
(2) weighing 0.08g of carbomer-940, adding 0.6g of glycerol for wetting, adding distilled water to 10.8g, stirring uniformly, and standing for 24h to fully swell to obtain a gel matrix;
(3) slowly adding the cinnamomum camphora extract micro emulsion into a gel matrix (wherein the mass ratio of the cinnamomum camphora extract micro emulsion to the gel matrix is 3: 7), uniformly stirring, dropwise adding a triethanolamine solution with the volume fraction of 20% to adjust the pH value to 5.43, and centrifuging at 4000r/min for 5min to remove bubbles to obtain the cinnamomum camphora extract micro emulsion gel.
Evaluation shows that the cinnamomum camphora extract microemulsion gel prepared in the example 3 has 17 points of appearance, 17 points of viscosity, 16 points of greasiness, 17 points of spreadability and 18 points of moisture retention rate; the total preparation fraction for performance evaluation was 85 points.
As can be seen from the performance scores of the camphor extract microemulsion gels prepared in the examples 1-3, the scores are all over 85 points. The ingredients of the cinnamomum camphora extract micro-emulsion and the gel matrix and the proportion of the two are in the range of the invention, the total score of the obtained cinnamomum camphora extract micro-emulsion gel is more than 85 minutes, and the cinnamomum camphora extract micro-emulsion gel with very excellent performance is obtained. The camphor extract microemulsion prepared under the conditions of the example 1 has the best performance.
Comparative example 1 preparation of Cinnamomum camphora extract microemulsion gel
(1) Weighing 0.3g of cinnamomum camphora extract, adding the cinnamomum camphora extract into 0.5g of olive oil, adding 0.25g of tween-801.25 g and 1, 2-propylene glycol, heating in a water bath at 30 ℃ until the materials are completely dissolved, uniformly stirring to obtain an oily mixture, slowly dripping 5mL of distilled water into the oily mixture under the constant-temperature magnetic stirring at 30 ℃ for emulsification to obtain cinnamomum camphora extract microemulsion;
(2) weighing 0.084g of carbomer-940, adding 0.525g of glycerol for wetting, adding distilled water to 10.8g, stirring uniformly, and standing for 24h to fully swell to obtain a gel matrix;
(3) slowly adding the cinnamomum camphora extract micro emulsion into a gel matrix (wherein the mass ratio of the cinnamomum camphora extract micro emulsion to the gel matrix is 4: 6), uniformly stirring, dropwise adding a triethanolamine solution with the volume fraction of 20% to adjust the pH value to 5.43, and centrifuging at 4000r/min for 5min to remove bubbles to obtain the cinnamomum camphora extract micro emulsion gel.
Comparative example 1 differs from example 1 in the composition of the microemulsion system. In comparative example 1, a microemulsion system consisting of oil phase olive oil, emulsifier Tween-80 and co-emulsifier 1, 2-propylene glycol is adopted, while in example 1, a microemulsion system consisting of oil phase castor oil, emulsifier polyoxyethylene castor oil and co-emulsifier 1, 2-propylene glycol is adopted; the other conditions were the same.
Evaluation shows that the cinnamomum camphora extract microemulsion gel prepared in the comparative example 1 has 13 points of appearance, 15 points of viscosity, 12 points of greasiness, 14 points of spreadability and 15 points of moisture retention rate; the total preparation fraction for performance evaluation was 69 points. Therefore, the camphor extract microemulsion gel prepared in the comparative example 1 has the performance score far smaller than 97 points of example 1; the result shows that the system composition of the cinnamomum camphora extract microemulsion in the step (1) has a decisive influence on the performance of the cinnamomum camphora extract microemulsion gel, and the cinnamomum camphora extract microemulsion gel with excellent performance can be prepared only under the microemulsion system consisting of the oil-phase castor oil, the emulsifier polyoxyethylene castor oil and the co-emulsifier 1, 2-propylene glycol.
Comparative example 2 preparation of Cinnamomum camphora extract microemulsion gel
(1) Weighing 0.3g of cinnamomum camphora extract, adding the cinnamomum camphora extract into 0.5g of ethyl oleate, adding 0.25g of tween-801.25 g and 1, 2-propylene glycol, heating in a water bath at 30 ℃ until the materials are completely dissolved, uniformly stirring to obtain an oily mixture, slowly dripping 5mL of distilled water into the oily mixture under the constant-temperature magnetic stirring at 30 ℃ for emulsification to obtain cinnamomum camphora extract microemulsion;
(2) weighing 0.084g of carbomer-940, adding 0.525g of glycerol for wetting, adding distilled water to 10.8g, stirring uniformly, and standing for 24h to fully swell to obtain a gel matrix;
(3) slowly adding the cinnamomum camphora extract micro emulsion into a gel matrix (wherein the mass ratio of the cinnamomum camphora extract micro emulsion to the gel matrix is 4: 6), uniformly stirring, dropwise adding a triethanolamine solution with the volume fraction of 20% to adjust the pH value to 5.43, and centrifuging at 4000r/min for 5min to remove bubbles to obtain the cinnamomum camphora extract micro emulsion gel.
Comparative example 2 differs from example 1 in the composition of the microemulsion system. In comparative example 2, a microemulsion system consisting of oil phase ethyl oleate, emulsifier Tween-80 and co-emulsifier 1, 2-propylene glycol is adopted, while in example 1, a microemulsion system consisting of oil phase castor oil, emulsifier polyoxyethylene castor oil and co-emulsifier 1, 2-propylene glycol is adopted; the other conditions were the same.
Evaluation shows that the cinnamomum camphora extract microemulsion gel prepared in the comparative example 2 has 15 points of appearance, 13 points of viscosity, 12 points of greasiness, 12 points of spreadability and 15 points of moisture retention rate; the total preparation fraction for the performance evaluation was 67 points. Therefore, the cinnamomum camphora extract microemulsion gel prepared in the comparative example 2 has the performance score far smaller than 97 points of the embodiment 1; the result shows that the system composition of the cinnamomum camphora extract microemulsion in the step (1) has a decisive influence on the performance of the cinnamomum camphora extract microemulsion gel, and the cinnamomum camphora extract microemulsion gel with excellent performance can be prepared only under the microemulsion system consisting of the oil-phase castor oil, the emulsifier polyoxyethylene castor oil and the co-emulsifier 1, 2-propylene glycol.
Comparative example 3 preparation of Cinnamomum camphora extract microemulsion gel
(1) Weighing 0.2g of cinnamomum camphora extract, adding the cinnamomum camphora extract into 0.3g of castor oil, then adding 1.5g of polyoxyethylene castor oil (EL) and 0.35g of 1, 2-propylene glycol, heating in a water bath at 30 ℃ until the materials are completely dissolved, uniformly stirring to obtain an oily mixture, slowly dripping 5mL of distilled water into the oily mixture under the constant-temperature magnetic stirring at 30 ℃ for emulsification to obtain cinnamomum camphora extract microemulsion;
(2) weighing 0.084g of carbomer-940, adding 0.525g of glycerol for wetting, adding distilled water to 10.8g, stirring uniformly, and standing for 24h to fully swell to obtain a gel matrix;
(3) slowly adding the cinnamomum camphora extract micro emulsion into a gel matrix (wherein the mass ratio of the cinnamomum camphora extract micro emulsion to the gel matrix is 4: 6), uniformly stirring, dropwise adding a triethanolamine solution with the volume fraction of 20% to adjust the pH value to 5.43, and centrifuging at 4000r/min for 5min to remove bubbles to obtain the cinnamomum camphora extract micro emulsion gel.
The comparative example 3 is different from the example 1 in the mass ratio of the cinnamomum camphora extract, the oil phase, the emulsifier and the co-emulsifier in the step of preparing the cinnamomum camphora extract microemulsion of the step (1).
Evaluation shows that the cinnamomum camphora extract microemulsion gel prepared in the comparative example 3 has 15 points of appearance, 16 points of viscosity, 14 points of greasiness, 15 points of spreadability and 17 points of moisture retention rate; the total preparation fraction for performance evaluation was 77 points. Therefore, the camphor extract micro-emulsion gel prepared in the comparative example 3 has the performance score far smaller than 97 points of example 1; the mass ratio of the cinnamomum camphora extract, the oil phase, the emulsifier and the co-emulsifier in the step (1) of preparing the cinnamomum camphora extract microemulsion plays an important role in the performance of the cinnamomum camphora extract microemulsion gel, and the mass ratio of the cinnamomum camphora extract, the oil phase, the emulsifier and the co-emulsifier in the step (1) of preparing the cinnamomum camphora extract microemulsion gel with excellent performance can be prepared only under the proportion of the invention.
Comparative example 4 preparation of Cinnamomum camphora extract microemulsion gel
(1) Weighing 0.2g of cinnamomum camphora extract, adding the cinnamomum camphora extract into 0.3g of castor oil, then adding 1.5g of polyoxyethylene castor oil (EL) and 0.35g of 1, 2-propylene glycol, heating in a water bath at 30 ℃ until the materials are completely dissolved, uniformly stirring to obtain an oily mixture, slowly dripping 5mL of distilled water into the oily mixture under the constant-temperature magnetic stirring at 30 ℃ for emulsification to obtain cinnamomum camphora extract microemulsion;
(2) weighing 0.07g of carbomer-940, adding 0.65g of glycerol for wetting, adding distilled water to 10.8g, stirring uniformly, standing for 24h to fully swell to obtain a gel matrix;
(3) slowly adding the cinnamomum camphora extract micro emulsion into a gel matrix (wherein the mass ratio of the cinnamomum camphora extract micro emulsion to the gel matrix is 6: 4), uniformly stirring, dropwise adding a triethanolamine solution with the volume fraction of 20% to adjust the pH value to 5.43, and centrifuging at 4000r/min for 5min to remove bubbles to obtain the cinnamomum camphora extract micro emulsion gel.
The comparative example 4 is different from the example 1 in the mass and dosage ratios of the cinnamomum camphora extract, the oil phase, the emulsifier and the co-emulsifier in the step of preparing the cinnamomum camphora extract microemulsion of the step (1); the gel matrix in the step (2) has different component ratios; in the step (3), the mass and dosage ratios of the camphor extract micro-emulsion and the gel matrix are different.
Evaluation shows that the cinnamomum camphora extract microemulsion gel prepared in the comparative example 4 has 12 points of appearance, 14 points of viscosity, 11 points of greasiness, 13 points of spreadability and 15 points of moisture retention rate; the total preparation fraction for performance evaluation was 65 points. Therefore, the camphor extract microemulsion gel prepared in the comparative example 4 has the performance score far smaller than 97 points of example 1; this shows that the amount of all the components in the steps (1), (2) and (3) has an important influence on the performance of the camphor extract microemulsion gel; the camphor extract micro-emulsion gel with excellent performance can be prepared only under the proportion of the components.
Experimental example 1 microemulsion gel antibacterial ability test of Cinnamomum camphora extract
The antimicrobial capacity of the cinnamomum camphora extract microemulsion gel prepared in example 1 was measured by the oxford cup method
Oxford cup method (oxford cup inner diameter 6.0 mm): sucking 100 mul of bacterial suspension of staphylococcus aureus, surface staphylococcus and escherichia coli by using a liquid transfer gun, putting the bacterial suspension on an instant agar plate, uniformly coating, drying at room temperature for 5min, respectively putting 3 oxford cups at marked positions, dripping prepared cinnamomum camphora extract micro-emulsion gel and commercially available gel diluent into the oxford cups according to the number, repeating each sample by 100 mul for two times, placing for 30min, culturing in a constant-temperature incubator at 37 ℃ for 18-24 h, observing the size of a bacteriostatic circle, and averaging. The evaluation of the bacteriostatic effect is as follows: the diameter of the bacteriostatic ring is more than 7mm, which indicates that the product has bacteriostatic action; the diameter of the antibacterial ring is not more than 7mm, which indicates that the product has no antibacterial effect.
The results show that the diameters of the cinnamomum camphora extract microemulsion gel and the commercially available tea tree gel for inhibiting staphylococcus aureus are respectively (7.45 +/-0.03) mm and (7.46 +/-0.03) mm, which are both larger than 7mm, the cinnamomum camphora extract microemulsion gel and the commercially available tea tree gel have an inhibiting effect on staphylococcus aureus, and the cinnamomum camphora extract microemulsion gel is weaker than the commercially available tea tree gel in the staphylococcus aureus resistance. The antibacterial zones of the cinnamomum camphora extract micro-emulsion gel and the commercially available tea tree gel to surface staphylococcus are (7.56 +/-0.03) mm and are larger than 7mm, which shows that the cinnamomum camphora extract micro-emulsion gel has the antibacterial effect consistent with that of the commercially available tea tree gel in the surface staphylococcus resistance. The diameters of the antibacterial zones of the cinnamomum camphora extract micro-emulsion gel and commercially available tea tree gel escherichia coli are respectively (7.46 +/-0.03) and (7.58 +/-0.02), which shows that the cinnamomum camphora extract micro-emulsion gel is weaker than the commercially available tea tree gel in the escherichia coli resistance. The above proves that the cinnamomum camphora extract microemulsion has antibacterial performance which is weak or equivalent to the commercially available tea tree gel.
Experimental example 2 measurement of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of Cinnamomum camphora extract microemulsion gel
The broth microdilution protocol was recommended by the American Committee for standardization in clinical laboratories (CLSI-2012). A disposable sterile 96-well U-shaped bottom plate is prepared by adding 100. mu.l of a common broth to each well and adding 100. mu.l of a medium to the 12 th well in a total of 200. mu.l. Adding 100 μ l of Cinnamomum camphora extract microemulsion gel (prepared in example 1) with content of 4.2% and commercial tea tree gel diluent into the 1 st well of each row, sucking 100 μ l to the 2 nd well, mixing, sucking 100 μ l to the 3 rd well, diluting at equal ratio to the 10 th well, sucking 100 μ l from the 10 th well, discarding, inoculating, and collecting the solution with concentration of 104Bacterial suspensions of CFU/mL of various strains are respectively added into the 1 st to 11 th holes, each hole is 100 mu l of bacterial suspension, the 11 th hole is filled with bacterial liquid and culture medium as positive control, and the 12 th hole is filled with growth control (negative control) without medicine. To this end, the final drug content was 2.1%, 1.05%, 0.53%, 0.26%, 0.13%, 0.066%, 0.033%, 0.016%, 0.008%, 0.004%. The whole operation process is aseptic operation. Culturing in a constant temperature incubator at 37 ℃ for 18-24 h. The solution in the micropores is clear and transparent when observed by naked eyes under a black background, namely, the bacteria are completely inhibitedThe lowest drug concentration for growth is the MIC value of the substance, and the experiment for obvious bacterial growth in the positive control wells is meaningful when the solution in the negative control wells is clear and transparent. Two replicates were repeated.
Preparing blank test tubes 20 tubes, wherein the reference numbers are 1-9 and A-J respectively, adding 1ml of culture medium into each tube, adding 1ml of contrast solution into the tube No. 1, uniformly mixing, adding 1ml of contrast solution into the tube No. 2, sequentially adding the contrast solution into the tube No. 7, discarding 1ml of the tube No. 7, adding blank solution into the tube No. 8, discarding 1ml, adding 0.1ml of bacterial suspension into the tubes 1-9, wherein the tube No. 8 is blank control, the tube No. 9 is positive control, and the tube No. 10 is negative control. A sample solution was added as above. The experiment was performed in a biosafety cabinet, plugged with a rubber stopper and incubated anaerobically at 37 ℃ for 24 hours. And (6) observing the result. The experiment was repeated three times.
And respectively inoculating the broth in which bacteria do not grow in the MIC measurement to an instant common agar plate, marking, culturing at 37 ℃ for 18-24 h, and observing, wherein the concentration of the drug which grows aseptically on the plate is the Minimum Bactericidal Concentration (MBC) of the drug.
The experimental results show that: the cinnamomum camphora extract micro-emulsion gel and the commercially available tea tree gel have bacteriostatic action, but the MIC value and the MBC value of the cinnamomum camphora extract micro-emulsion gel to staphylococcus aureus are larger than those of the commercially available tea tree gel, which indicates that the bacteriostatic action of the cinnamomum camphora extract micro-emulsion gel is more obvious; the MIC value of the commercially available tea tree gel to the staphylococcus epidermidis is smaller than that of the cinnamomum camphora extract micro-emulsion gel, but the MBC value is consistent; the MIC value and the MBC value of the cinnamomum camphora extract micro-emulsion gel to escherichia coli are consistent with those of the commercially available tea tree gel, and the fact that the cinnamomum camphora extract micro-emulsion gel and the commercially available tea tree gel have the same bacteriostatic effect on the escherichia coli is shown. The experiments show that the micro-emulsion gel of the cinnamomum camphora extract has weak or equivalent bacteriostatic performance to the commercially available tea tree gel.
Experimental example 3 Effect of Cinnamomum camphora extract microemulsion gel on egg white-induced foot swelling
30 mice with normal and undamaged toes were randomly divided into a normal saline group (blank control), a dexamethasone group (positive control), and a cinnamomum camphora extract microemulsion gel (prepared in example 1) at low, medium, and high doses, and each group had 6 mice. Before the experiment, the thickness of the right hind limb and the foot sole of each group of mice is measured by a vernier caliper for 3 times respectively,the average was taken as the normal toe volume. The medicine is applied to the right hind instep of a mouse, 2ml of normal saline is applied to a normal saline group, 50mg of medicine is applied to a dexamethasone group, 25mg of medicine is applied to a camphor extract micro-emulsion gel low-dose group, 50mg of medicine is applied to a camphor extract micro-emulsion gel medium-dose group, 100mg of medicine is applied to a camphor extract micro-emulsion gel high-dose group, 1 time of medicine administration is carried out every 1 hour, and 3 times of medicine administration are carried out. 30min after the last administration, 0.1mL of 10% fresh egg white solution (freshly prepared with physiological saline) is injected subcutaneously to the plantar region of the right hind paw of the mouse per mouse, so that inflammation and swelling are caused. The thickness of the right foot sole is measured at 0, 0.5, 1 and 2h after inflammation, the average value is obtained by three measurements, and the difference between the thickness of the right foot and the thickness before and after egg white liquid injection is used as the swelling degree of the foot. Inputting the experimental data of the acute inflammation model influence caused by the cinnamomum camphora extract microemulsion gel into SPSS22.0 statistical analysis software, wherein the mean value plus or minus standard deviation is adopted in each group of data
Showing that the multiple groups of mean comparisons adopt one-factor variance analysis, pairwise comparisons among the groups, LSD test and Duncan test for carrying out significance analysis of differences among the multiple groups of samples, P<A difference of 0.05 is statistically significant. The origin8.0 is used for drawing, the time is used as an abscissa, the mean +/-standard deviation is used as an ordinate, and a curve of the swelling degree of the foot sole of the mouse along with the inflammation-causing time is drawn. The results show that compared with the normal saline group, the cinnamomum camphora extract microemulsion gel can relieve the swelling of the feet of the mice caused by fresh egg white in 1 hour and 2 hours in the low, medium and high groups<0.05) and has a certain dose-dependent relation on the inhibition rate of swelling, and the difference between the dexamethasone control group and the normal saline group has no statistical significance (P)>0.05), wherein the high-dose inhibition effect of the cinnamomum camphora extract microemulsion gel is better than that of a positive drug dexamethasone (P) at 2h<0.01), has significant difference, and the low, medium and high doses have statistical significance on the reduction effect of the foot swelling (P)<0.05), showing that the camphor extract micro-emulsion gel has anti-inflammatory effect in low, medium and high dose groups, and the anti-inflammatory effect and the effect of the micro-emulsion gel are better than those of the dexamethasone group.
From the figure 1, the foot swelling of each group after egg white injection is obvious, and after the camphor extract micro-emulsion gel and dexamethasone are smeared, the foot swelling of the mice is obviously inhibited. Before 0.5h, the camphor extract micro-emulsion gel high-dose group has approximately the same effect as the dexamethasone group, after 0.5h, the foot swelling inhibition effect of the dexamethasone group gradually becomes worse, and the camphor extract micro-emulsion gel low, medium and high-dose groups have gradually better effects than the dexamethasone group; after 1h, the effect of inhibiting egg white-induced paw edema was ordered (from superior to inferior): the camphor tree extract microemulsion gel high-dose group > the medium-dose group > the low-dose group > the dexamethasone group > the normal saline group. The experiments show that the camphor extract microemulsion gel has obvious effects on egg white induced foot swelling in low, medium and high dose groups, and is superior to the dexamethasone group.
Claims (10)
1. The preparation method of the cinnamomum camphora extract microemulsion gel is characterized by comprising the following steps:
the preparation method of the cinnamomum camphora extract microemulsion comprises the following steps: weighing the camphor extract, adding the camphor extract into the oil phase, then adding the emulsifier and the co-emulsifier, and stirring uniformly under a heating condition to obtain an oily mixture; adding water into the oily mixture under stirring for emulsification to obtain Cinnamomum camphora extract microemulsion;
the preparation steps of the gel matrix are as follows: weighing carbomer-940, adding glycerol for wetting, adding water, stirring, and standing to swell completely to obtain gel matrix;
mixing the cinnamomum camphora extract micro emulsion and a gel matrix: adding the cinnamomum camphora extract micro emulsion into a gel matrix, uniformly stirring, adjusting the pH value to 5-6, and centrifuging to obtain the cinnamomum camphora extract micro emulsion gel.
2. The method for preparing the cinnamomum camphora extract microemulsion gel according to claim 1, wherein the oil phase in the step of preparing the cinnamomum camphora extract microemulsion is castor oil.
3. The method for preparing the cinnamomum camphora extract microemulsion gel according to claim 1, wherein the emulsifier in the step of preparing the cinnamomum camphora extract microemulsion is polyoxyethylene castor oil.
4. The method for preparing the cinnamomum camphora extract microemulsion gel according to claim 1, wherein the co-emulsifier in the step of preparing the cinnamomum camphora extract microemulsion is 1, 2-propylene glycol.
5. The preparation method of the cinnamomum camphora extract microemulsion gel according to claim 1, wherein in the preparation step of the cinnamomum camphora extract microemulsion, the mass ratio of the cinnamomum camphora extract to the oil phase to the emulsifier to the co-emulsifier is 0.25-0.35: 0.4-0.6: 1.1-1.4: 0.2 to 0.3.
6. The preparation method of the cinnamomum camphora extract microemulsion gel according to claim 5, wherein in the preparation step of the cinnamomum camphora extract microemulsion, the mass ratio of the cinnamomum camphora extract to the oil phase to the emulsifier to the co-emulsifier is 0.3: 0.5: 1.25: 0.25.
7. the preparation method of the cinnamomum camphora extract microemulsion gel according to claim 1, wherein in the preparation step of the gel matrix, the mass ratio of carbomer-940 to glycerin to water is 0.08-0.09: 0.5-0.6: 10.0 to 11.0.
8. The preparation method of the cinnamomum camphora extract microemulsion gel according to claim 1, wherein the mass and dosage ratio of the cinnamomum camphora extract microemulsion to the gel matrix in the step of mixing the cinnamomum camphora extract microemulsion with the gel matrix is 3-4: 6 to 7.
9. The preparation method of the cinnamomum camphora extract microemulsion gel according to claim 1, wherein the mass ratio of the cinnamomum camphora extract microemulsion to the gel matrix in the step of mixing the cinnamomum camphora extract microemulsion with the gel matrix is 4: 6.
10. the camphor extract microemulsion gel prepared by the preparation method of any one of claims 1 to 9.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115252662A (en) * | 2022-07-19 | 2022-11-01 | 嘉应学院 | A Borneolum extract, its preparation method and application in preparing antibacterial preparation |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102908441A (en) * | 2011-08-02 | 2013-02-06 | 北京中医药大学 | Pain relieving micro-emulsion pharmaceutical composition gel ointment and preparation method thereof |
| CN103655459A (en) * | 2013-12-19 | 2014-03-26 | 中国药科大学 | Multifunctional microemlusion gel preparation and preparation process thereof |
| CN103735620A (en) * | 2014-01-03 | 2014-04-23 | 河南中医学院 | Nano-emulsion in-situ gel for treating haemorrhoids |
| CN105362351A (en) * | 2015-12-16 | 2016-03-02 | 成都昊健生物科技有限责任公司 | Medicine composition for treating cutaneous pruritus disease and application thereof |
| CN107320699A (en) * | 2017-06-30 | 2017-11-07 | 中国中医科学院中药研究所 | A kind of Chinese medicine compound prescription micro emulsion gels and its production and use |
| CN109432384A (en) * | 2018-10-16 | 2019-03-08 | 罗永恒 | A kind of camphor oil micro emulsion gel and preparation method thereof with antitumor antiinflammation |
-
2019
- 2019-10-12 CN CN201910966734.8A patent/CN110693832A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102908441A (en) * | 2011-08-02 | 2013-02-06 | 北京中医药大学 | Pain relieving micro-emulsion pharmaceutical composition gel ointment and preparation method thereof |
| CN103655459A (en) * | 2013-12-19 | 2014-03-26 | 中国药科大学 | Multifunctional microemlusion gel preparation and preparation process thereof |
| CN103735620A (en) * | 2014-01-03 | 2014-04-23 | 河南中医学院 | Nano-emulsion in-situ gel for treating haemorrhoids |
| CN105362351A (en) * | 2015-12-16 | 2016-03-02 | 成都昊健生物科技有限责任公司 | Medicine composition for treating cutaneous pruritus disease and application thereof |
| CN107320699A (en) * | 2017-06-30 | 2017-11-07 | 中国中医科学院中药研究所 | A kind of Chinese medicine compound prescription micro emulsion gels and its production and use |
| CN109432384A (en) * | 2018-10-16 | 2019-03-08 | 罗永恒 | A kind of camphor oil micro emulsion gel and preparation method thereof with antitumor antiinflammation |
Non-Patent Citations (2)
| Title |
|---|
| 叶小玲等: "梅州香樟挥发油成分及其脂质体凝胶的制备", 《山东化工》 * |
| 叶小玲等: "梅州香樟挥发油抗炎活性试验", 《广东化工》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115252662A (en) * | 2022-07-19 | 2022-11-01 | 嘉应学院 | A Borneolum extract, its preparation method and application in preparing antibacterial preparation |
| CN115252662B (en) * | 2022-07-19 | 2023-07-18 | 嘉应学院 | Plum slice extract, preparation method and application thereof in preparation of antibacterial preparation |
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