CN110672847A - Cell sensor based on silver coordination polymer assembly and application of cell sensor in circulating tumor cell detection - Google Patents
Cell sensor based on silver coordination polymer assembly and application of cell sensor in circulating tumor cell detection Download PDFInfo
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Abstract
The invention discloses a cell sensor based on a silver coordination polymer assembly and application thereof in circulating tumor cell detection, which comprises the following specific steps: (1) by Ag (I) and-NH2Complexing, Ag (I) -Ag (I) interactions and Ag (I) and-SH interactions synthesize a CRGD-Ag (I) coordination polymer; (2) the polymerization is modified on the surface of a gold electrode through the interaction of Au and-SH, the specific combination of RGD and cell surface integrin is utilized, and the polymer modified electrode captures circulating tumor cells by utilizing the special action, so that the sensor prepared by modification is completed. At 5mM [ Fe (CN)6]3‑/4‑Detecting electrochemical impedance response in a solution containing 0.1M KCl, and detecting the change of the electrochemical response intensity of the sensor by changing the concentration of MCF-7 cells to realize the detection of the concentration. Its advantages are high sensitivity, high speed, accurate and reliable result and low cost.
Description
Technical Field
The invention relates to a cell sensor and a detection method thereof, in particular to a cell sensor based on a silver coordination polymer assembly and application thereof in circulating tumor cell detection, belonging to the technical field of functional biomaterials and biosensing.
Background
Cancer is well known to be one of the biggest killers of human health. The main cause of death of cancer patients is cancer invasion and metastasis. Early detection may help the clinic to take timely measures to inhibit cancer metastasis. Therefore, early monitoring of cancer metastasis is of great clinical significance. An excellent marker is essential for early detection of cancer metastasis. Circulating tumor cells are cancer cells that shed from the tumor in situ into the blood and are considered to be a biomarker with great marker value for tumor metastasis. The detection and analysis of circulating tumor cells are not only of great significance for the early diagnosis of cancer metastasis, but also of great significance for the rapid assessment of prognosis recurrence and the effect of chemotherapeutic drugs. In addition, the collection and analysis of the circulating tumor cells in the blood of the patient are beneficial to individualized treatment, clinical drug screening, drug resistance monitoring, development of new tumor drugs and the like. Therefore, the detection of circulating tumor cells is essential.
Currently, many traditional methods are available at home and abroad for detecting circulating tumor cells, such as cytological detection, fluorescence imaging, magnetic resonance imaging, computed tomography, radiography, and ultrasound. However, most of these methods have more or less drawbacks, such as high cost, long time consumption, low sensitivity and insufficient specificity or the need for professional operators, which limits the clinical application of the conventional methods. The development of electrochemical biosensors has provided new tools and new methods for effectively solving these problems. Electrochemical biosensors consist of two main parts, namely a biorecognition element and a signal converter. The primary function of the signal converter is to convert the biological recognition effect into a detectable signal. The substance to be detected acts on the biological recognition element, then the signal converter converts the substance to be detected into a detectable signal, and finally a corresponding electrochemical signal is displayed on a computer, so that a new direction is provided for the development of the circulating tumor electrochemical sensor.
The coordination polymer takes metal ions as connecting points and is connected through organic ligands to form a porous framework structure with high porosity and high specific surface area. This unique porous structure has attracted considerable attention over the past few decades, and the number of literature reports relating to coordination polymers has also increased dramatically. In recent years, complex compounds have been rapidly developed in various fields as a branch of research of inorganic chemistry. Among them, silver coordination polymers are well studied in the fields of catalysis, photoelectric materials, gas adsorption and desorption, gas storage, etc., and research make internal disorder or usurp of silver coordination polymers in the biomedical fields of drug delivery, drug storage, imaging agents, bioprobes, etc. is still in the exploration stage. So far, the preparation and application of cell sensors based on silver coordination polymers are not found.
The invention designs a cell sensor based on a silver coordination polymer assembly and application thereof in circulating tumor cell detection, wherein Ag (I) and-NH are used2Complexation, Ag (I) -Ag (I) interactions and Ag (I) and-SH interactions synthesize a CRGD-Ag (I) coordination polymer. The polymerization is modified on the surface of a gold electrode through the interaction of Au and-SH, the specific combination of RGD and cell surface integrin is utilized, and the polymer modified electrode captures circulating tumor cells (taking MCF-7 cells as an example) by utilizing the special action, so that the electrochemical impedance signal is obviously increased, and the detection of the circulating tumor cells is realized. At present, no report related to the preparation and application of the circulating tumor cell electrochemical sensor based on the CRGD-Ag (I) coordination polymer is found at home and abroad.
Disclosure of Invention
The invention aims to solve the technical problem of providing a cell sensor based on a silver coordination polymer assembly body, which has high sensitivity, high detection speed, accurate and reliable result and low cost, and an application thereof in circulating tumor cell detection.
The technical scheme adopted by the invention for solving the technical problems is as follows: a cell sensor based on a silver coordination polymer assembly and application thereof in circulating tumor cell detection specifically comprise the following steps:
(1) CRGD-Ag (I) coordination polymer synthesis
Taking AgNO3Adding the solution (0.5-1.5 mM, 2-8 μ L) and CRGD (0.5-1.5 mM, 2-8 μ L) into 50-100 μ L distilled water, and shaking (100-1000 rpm) on a 30 ℃ constant temperature magnetic stirrer for reaction for about 5-15 min. To obtain the synthesis of the CRGD-Ag (I) coordination polymer.
(2) Preparation of the sensor
Preparation of Au
Gold electrodes (Au, diameter 3mm) were first sueded with Al having particle sizes of 1.0, 0.3 and 0.05 μm2O3Polishing the powder, carrying out ultrasonic cleaning on the electrode obtained after polishing in an ultrasonic cleaning instrument by using secondary distilled water for 5-10 min, and using N for the electrode after ultrasonic cleaning2Blow-drying, and marking as Au.
Preparation of CRGD-Ag (I)/Au sensor
And (3) dripping 5-15 mu L of the prepared CRGD-Ag (I) coordination polymer solution onto the surface of Au, and incubating for 3-16 h at room temperature to finish modification, wherein the solution is marked as CRGD-Ag (I)/Au.
Preparation of cells/CRGD-Ag (I)/Au
Taking 5-15 mu L of prepared series of MCF-7 cell suspensions (10-10)8one/mL), dripping on the surface of CRGD-Ag (I)/Au, and incubating for 0.1-1 h at 30-42 ℃. Labeled Cells/CRGD-Ag (I)/Au.
During the preparation of Cells/CRGD-Ag (I)/Au, MCF-7 cell concentration was varied, and a series of cell sensors were prepared to detect electrochemical impedance responses at different MCF-7 cell concentrations.
The cell sensor based on the silver coordination polymer assembly and the application thereof in circulating tumor cell detection adopt an electrochemical impedance method, and the frequency range is as follows: 10-2～105Hz, amplitude: 2mV at 5mM [ Fe (CN)6]3-/4-Obtaining a resistance change curve in a solution containing 0.1MKCl, obtaining a series of electrical impedance sizes with different MCF-7 cell concentrations, establishing a relation between resistance response signal intensity and the MCF-7 cell concentration, and determining the concentration of the MCF-7 cells in the sample to be detected according to the quantitative relation between the resistance response signal intensity and the MCF-7 cell concentration.
The invention principle is as follows: the invention is an electrochemical cell sensor, which is prepared by Ag (I) and-NH2The complexation effect,Ag (I) -Ag (I) interaction and Ag (I) and-SH interaction a CRGD-Ag (I) coordination polymer was synthesized. The polymerization is modified on the surface of a gold electrode through the interaction of Au and-SH, MCF-7 cells are specifically combined with CRGD-Ag (I) coordination polymer and cell surface integrin, and the MCF-7 cells are detected through the change of electrochemical impedance signals. Based on the method, a simple, rapid, high-sensitivity, high-selectivity and label-free circulating tumor cell analysis method is constructed.
Compared with the prior art, the invention has the advantages that: the invention constructs a cell sensor based on a silver coordination polymer assembly and application thereof in circulating tumor cell detection. First, by Ag (I) and-NH2Complexation, Ag (I) -Ag (I) interactions and Ag (I) and-SH interactions synthesize a CRGD-Ag (I) coordination polymer. The polymerization is modified on the surface of a gold electrode through Au and-SH interaction, and then the electrochemical response of the sensor to MCF-7 cells with different concentrations is detected by an electrochemical impedance method. It is clear that the greater the concentration of the target, the more pronounced the resistive response, within a certain range of concentrations. The experimental result shows that the size of the resistor and the concentration of the target object are in a linear relationship in a certain range, and the detection of the target object is realized. The advantages are that:
(1) the sensitivity is high. The linear correlation equation of the resistance response of the sensor to the MCF-7 cell concentration is that y is 678lgCMCF-7-869，R20.9953, detection range: 10 to 5 x 107The limit of cell/mL detection is 2Cells/mL, which indicates that the sensor realizes high-sensitivity detection on MCF-7 Cells.
(2) The accuracy is high. The recovery rate is between 90% and 110%.
(3) The cost is low. The invention can realize high-sensitivity detection of MCF-7 cells by only consuming a small amount of materials and reagents.
In conclusion, the novel electrochemical sensor is constructed for detecting the circulating tumor cells, has the advantages of high sensitivity, good selectivity, simplicity in operation, rapidness in analysis, easiness in operation and the like, can realize detection of MCF-7 cells with different concentrations, and has a good application prospect.
Drawings
FIG. 1 is a comparative illustration of electrode modification for a sensor according to the present invention;
FIG. 2 is a diagram showing the effective binding of the sensor of the present invention to MCF-7 cells;
FIG. 3 is a graph showing the detection of different concentrations of MCF-7 cells by the sensor of the present invention;
FIG. 4 is a standard graph of the detection of MCF-7 cells by the sensor of the present invention;
Detailed Description
The invention is described in further detail below with reference to the accompanying examples.
EXAMPLE 1 preparation of the sensor
(1) CRGD-Ag (I) coordination polymer synthesis
Taking AgNO3The solution (1mM, 8. mu.L) and CRGD (1mM, 8. mu.L) were added to 100. mu.L of distilled water, and reacted for about 5min with shaking (500rpm) on a constant temperature magnetic stirrer at 30 ℃. To obtain the synthesis of the CRGD-Ag (I) coordination polymer.
(2) Preparation of the sensor
Preparation of Au
Gold electrodes (Au, diameter 3mm) were first sueded with Al having particle sizes of 1.0, 0.3 and 0.05 μm2O3Polishing the powder, ultrasonically cleaning the polished electrode with secondary distilled water in an ultrasonic cleaner for 5min, and cleaning with N2Blow-drying, and marking as Au.
Preparation of CRGD-Ag (I)/Au sensor
Dripping 10 mu L of the prepared CRGD-Ag (I) coordination polymer solution on the surface of Au, incubating for 4h at room temperature to finish modification, and marking as CRGD-Ag (I)/Au.
For comparison, we drop CRGD (1mM, 8 μ L) directly onto the Au surface and incubate at room temperature for 4h, completing the modification, labeled CRGD/Au.
Preparation of cells/CRGD-Ag (I)/Au
Taking 10 mu L of a series of MCF-7 cell suspensions (10-10)8one/mL), dropped onto the surface of CRGD-Ag (I)/Au, and incubated at 37 ℃ for 0.5 h. Labeled Cells/CRGD-Ag (I)/Au.
Detecting the above four kinds of electricityPolar pair 5mM [ Fe (CN)6]3-/4-Electrical impedance response of solutions containing 0.1M KCl. As shown in FIG. 1, it can be seen that compared with the other three electrodes, the prepared sensor CRGD/Au has very significant electrochemical impedance response, which indicates that the polymer is stably and disorderly modified on the electrode surface, resulting in very large electrochemical impedance response, while the resistance of CRGD-Ag (I)/Au is smaller than that of CRGD/Au, because the addition of Ag (I) enables the CRGD in the polymer to be orderly arranged, and the Ag (I) with positive charge to a certain extent can reduce the resistance, thus facilitating the preparation of the impedance cell sensor, greatly reducing the background signal, and laying the foundation for the following cell adsorption experiment. As shown in FIG. 2, the electrochemical impedance response further increased as MCF-7 was specifically adsorbed by the polymer. The successful production of the cell sensor was demonstrated and was used to detect MCF-7 cells.
EXAMPLE 2 detection of MCF-7 cells at various concentrations
In the preparation of Cells/CRGD-Ag (I)/Au according to the preparation procedure of the sensor of example 1 above, the concentration of MCF-7 Cells was varied in the order: 0. 10, 1 × 102、1×103、5×103、1×104、5×104、1×105、5×105、1×106、5×106、1×107、5×107Cells/mL. The results are shown in FIG. 3, which illustrates that the electrochemical impedance response of the sensor is more obvious as the MCF-7 cell concentration is increased; as shown in FIG. 4, the linear correlation equation of the electrochemical impedance response of the sensor to the MCF-7 cell concentration is 678lgCMCF-7-869，R20.9953, detection range: 10 to 1 x 107cell/mL, detection limit of 2Cells/mL, shows that the sensor realizes high sensitivity detection on MCF-7 Cells.
Of course, the above description is not intended to limit the present invention, and the present invention is not limited to the above examples. Variations, modifications, additions and substitutions which may occur to those skilled in the art and which fall within the spirit and scope of the invention are also considered to be within the scope of the invention.
Claims (4)
1. Cell sensor based on silver coordination polymer assembly and cell sensorThe application of the probe in circulating tumor cell detection is characterized in that the mechanism is as follows: by Ag (I) and-NH2Complexation, Ag (I) -Ag (I) interactions and Ag (I) and-SH interactions synthesize a CRGD-Ag (I) coordination polymer. The polymerization is modified on the surface of a gold electrode through the interaction of Au and-SH, the specific combination of RGD and cell surface integrin is utilized, and the polymer modified electrode captures circulating tumor cells (taking MCF-7 cells as an example) by utilizing the special action, so that the electrochemical impedance signal is obviously increased, and the detection of the circulating tumor cells is realized. The concentration of the MCF-7 cells is changed to detect the change of the electrochemical response intensity of the sensor, so that the concentration of the MCF-7 cells is detected.
2. A cell sensor based on a silver coordination polymer assembly and application thereof in circulating tumor cell detection specifically comprise the following steps:
(1) CRGD-Ag (I) coordination polymer synthesis
Taking AgNO3Adding the solution (0.5-1.5 mM, 2-8 μ L) and CRGD (0.5-1.5 mM, 2-8 μ L) into 50-100 μ L distilled water, and shaking (100-1000 rpm) on a 30 ℃ constant temperature magnetic stirrer for reaction for about 5-15 min. To obtain the synthesis of the CRGD-Ag (I) coordination polymer.
(2) Preparation of the sensor
Preparation of Au
Gold electrodes (Au, diameter 3mm) were first sueded with Al having particle sizes of 1.0, 0.3 and 0.05 μm2O3Polishing the powder, carrying out ultrasonic cleaning on the electrode obtained after polishing in an ultrasonic cleaning instrument by using secondary distilled water for 5-10 min, and using N for the electrode after ultrasonic cleaning2Blow-drying, and marking as Au.
Preparation of CRGD-Ag (I)/Au sensor
And (3) dripping 5-15 mu L of the prepared CRGD-Ag (I) coordination polymer solution onto the surface of Au, and incubating for 3-16 h at room temperature to finish modification, wherein the solution is marked as CRGD-Ag (I)/Au.
Preparation of cells/CRGD-Ag (I)/Au
Taking 5-15 mu L of prepared series of MCF-7 cell suspensions (10-10)8one/mL) is dripped on the surface of CRGD-Ag (I)/AuAnd incubating for 0.1-1 h at 30-42 ℃. Labeled Cells/CRGD-Ag (I)/Au.
3. An electrochemical sensor according to claims 1-2, wherein: the silver coordination polymer assembly is applied to circulating tumor cell detection for the first time, the method is an electrochemical impedance method, and the frequency range is as follows: 10-2～105Hz, amplitude: 2mV at 5mM [ Fe (CN)6]3-/4Determination of the electrochemical impedance changes caused by MCF-7 cells in a solution containing 0.1M KCl.
4. The electrochemical sensor according to claims 1-3, can realize detection of MCF-7 cells with different concentrations, and the detection range is as follows: 10 to 1 x 107Cells/mL, detection limit of 2 Cells/mL.
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102818826A (en) * | 2012-07-21 | 2012-12-12 | 上海师范大学 | Electrochemical cell-based biosensor based on nanometer Ag@BSA biomimetic interface and preparation method thereof |
| US20130052131A1 (en) * | 2011-08-26 | 2013-02-28 | The Board Of Trustees Of The Leland Stanford Junior University | Nanoparticles, methods of making nanoparticles, and methods of use |
| CN103169982A (en) * | 2011-12-23 | 2013-06-26 | 中国科学院上海药物研究所 | Biological active peptide modified nano-silver and preparation method and applications thereof |
| CN103954663A (en) * | 2014-04-08 | 2014-07-30 | 同济大学 | Method for detecting cell proliferation activity by adopting cell electrode electrochemistry |
| CN104721821A (en) * | 2015-02-16 | 2015-06-24 | 天津大学 | Preparation method of targeted rare earth upconversion Au-core nanoparticle |
| CN105597099A (en) * | 2016-01-14 | 2016-05-25 | 南京邮电大学 | Multifunctional nanoprobe for targeting SERS (surface enhanced Raman scattering) imaging of tumor cells and preparation method thereof |
| CN107064259A (en) * | 2017-03-29 | 2017-08-18 | 宁波大学 | Based on coacetylase Au（I）The preparation method and application of the electrochemica biological sensor of coordination polymer |
| CN108061750A (en) * | 2017-11-30 | 2018-05-22 | 宁波大学 | Hydrogen peroxide and glucose detection are used for based on the proteinoid nano wire structure electrochemica biological sensor with electro catalytic activity |
-
2019
- 2019-09-26 CN CN201910970571.0A patent/CN110672847B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130052131A1 (en) * | 2011-08-26 | 2013-02-28 | The Board Of Trustees Of The Leland Stanford Junior University | Nanoparticles, methods of making nanoparticles, and methods of use |
| CN103169982A (en) * | 2011-12-23 | 2013-06-26 | 中国科学院上海药物研究所 | Biological active peptide modified nano-silver and preparation method and applications thereof |
| CN102818826A (en) * | 2012-07-21 | 2012-12-12 | 上海师范大学 | Electrochemical cell-based biosensor based on nanometer Ag@BSA biomimetic interface and preparation method thereof |
| CN103954663A (en) * | 2014-04-08 | 2014-07-30 | 同济大学 | Method for detecting cell proliferation activity by adopting cell electrode electrochemistry |
| CN104721821A (en) * | 2015-02-16 | 2015-06-24 | 天津大学 | Preparation method of targeted rare earth upconversion Au-core nanoparticle |
| CN105597099A (en) * | 2016-01-14 | 2016-05-25 | 南京邮电大学 | Multifunctional nanoprobe for targeting SERS (surface enhanced Raman scattering) imaging of tumor cells and preparation method thereof |
| CN107064259A (en) * | 2017-03-29 | 2017-08-18 | 宁波大学 | Based on coacetylase Au（I）The preparation method and application of the electrochemica biological sensor of coordination polymer |
| CN108061750A (en) * | 2017-11-30 | 2018-05-22 | 宁波大学 | Hydrogen peroxide and glucose detection are used for based on the proteinoid nano wire structure electrochemica biological sensor with electro catalytic activity |
Non-Patent Citations (1)
| Title |
|---|
| 杨维涛等: "基于蛋白和多肽为模板的贵金属纳米簇合成研究", 《化学学报》 * |
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