CN110672353B - Microcontact slice collection device for thin sections of biological tissue - Google Patents

Abstract

The invention discloses a micro-contact slice collection device for thin slices of biological tissue, which solves the problem that the adhesion and extrusion cause extrusion deformation to the sample during the process of adhesive tape adhering to the surface of the sample slice, resulting in uneven thickness and large deformation of the collected slices. question. The device includes a frame, a pressing rod, an adhesive tape, and an offset adjusting device. The pressing rod is arranged on the frame, the tape is movably wound around the pressing rod, and the offset adjusting device is connected with the pressing rod to adjust the four degrees of freedom. Adjust the space posture of the pressure rod, so as to keep the tape and the slice always constant during the collection process of the sliced sample, and reduce the deformation caused by the adhesive pressure of the adhesive tape as much as possible.

Description

Microcontact slice collection device for thin sections of biological tissue

technical field

The invention belongs to the field of biomedicine, and more particularly relates to a micro-contact slice collection device for thin slices of biological tissues.

Background technique

In the field of biomedicine, such as pathological research, virus marker verification and other applications, biological samples need to be mechanically cut to form thin sections, and then the thin sections are collected by a collection device, and then biological information is obtained through microscopic imaging. The existing tape-type slice collection method is generally to adhere the adhesive tape to the surface of the biological tissue sample first, and then use a cutting device to cut the sample surface. After the cutting is completed, the sample slice is also separated from the sample surface to realize the cutting and collection of the biological tissue sample. However, the problem with this method of collecting slices is that there is adhesion and extrusion in the process of adhering the sample slices with the tape, so the surface of the sample slices is squeezed and deformed, and the thickness of the collected sample slices is also uneven.

SUMMARY OF THE INVENTION

Aiming at the technical problems existing in the prior art that in the process of adhesive tape adhering to sample slicing, the adhesion and extrusion lead to the deformation of the sample, the deformation is large, and the thickness of the collected sample slices is uneven. The present invention provides a micro-contact method for thin sections of biological tissue The slice collection device includes a frame, a pressing rod, an adhesive tape, and an offset adjusting device, the pressing rod is arranged on the frame, and the tape is movably wound around the pressing rod, and the offset adjusting device It is connected with the pressing rod to adjust the spatial posture of the pressing rod in four degrees of freedom.

Preferably, the offset adjustment device includes a flexible adjustment mechanism and a displacement actuator, the flexible adjustment mechanism includes a first direction adjustment member and a second direction adjustment member, and the displacement actuator includes a first direction actuator , a second direction actuator, the first direction adjustment member cooperates with the first direction actuator to eliminate the offset in the first direction, and the second direction adjustment member is actuated with the second direction The device cooperates to eliminate the offset in the second direction.

Preferably, the first direction adjustment member has the same structure as the second direction adjustment member, and the direction is vertical. The first direction adjustment member includes four parallel flexible boards nested inside and outside, and the ends of the flexible boards are fixed to the on the frame so that the flexible board is displaced in a direction perpendicular to the board, but not displaced in a direction parallel to the board.

Preferably, the frame includes a first support and a second support that are symmetrical to each other, and also includes a cutting module, the cutting module includes a tool support rod and a tool, and one end of the tool support rod is fixed to the first support The support and the other end are fixed on the second support, and the cutter is arranged on the cutter support rod.

Preferably, the distance between the tangent line where the tape contacts the pressing rod and the cutter is constant.

Preferably, one or more first direction adjustment members, second direction adjustment members, first direction actuators, and second direction actuators are provided on the first support and the second support.

Preferably, the first support and the second support are provided with two first direction adjusting pieces and second direction adjusting pieces, one first direction actuator and one second direction actuator, two The first direction adjusting member and the second direction adjusting member are respectively arranged symmetrically, and the end of the pressing rod is arranged on the symmetrical point of the two first direction adjusting members or the second direction adjusting member, and the first direction adjusting member and the second direction adjusting member are symmetrical. One end of the direction actuator abuts against the first direction adjustment member, and one end of the second direction actuator abuts against the second direction adjustment member.

Preferably, the end of the tool support rod is detachably arranged on the first support and the second support through a fastening assembly.

Preferably, the fastening component includes a fixing block and a nut, and both the fixing block and the frame are provided with a groove for partially accommodating the tool support rod and a screw hole matched with the nut.

In general, compared with the prior art, the above technical solution conceived by the present invention can adjust the spatial posture of the pressing rod in four degrees of freedom by setting the offset adjusting device, that is, the four degrees of freedom of the pressing rod can be eliminated. In order to achieve precise control of the distance between the tape and the slices around the pressing rod, the slice deformation caused by the adhesive tape adhesion and extrusion process can be reduced as much as possible, and the thickness of the collected slices can also be guaranteed. Consistent.

Description of drawings

1 is a perspective view of a micro-contact slice collection device proposed by the present invention;

Fig. 2 is the structural schematic diagram of the flexible adjustment mechanism proposed by the present invention;

Fig. 3 is the schematic diagram of sample cutting in the present invention;

Fig. 4 is the principle schematic diagram of the flexible adjustment mechanism proposed by the present invention;

FIG. 5 is a schematic diagram of the four-degree-of-freedom direction of the pressure rod.

Throughout the drawings, the same reference numbers are used to refer to the same elements or structures, wherein:

1-frame; 2-pressing rod; 3-tape; 4-offset adjustment device; 5-flexible adjustment mechanism; 51-first direction adjustment member; 52-second direction adjustment member; 61-first direction actuation 62-second direction actuator; 7-first support; 8-second support; 9-cutting module; 10-tool support rod; 11-tool; 12-fixed block; 13-nut.

Detailed ways

In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention, but not to limit the present invention. In addition, the technical features involved in the various embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

As shown in FIG. 1 , this embodiment provides a micro-contact slice collection device for thin slices of biological tissue, which is characterized in that it includes a frame 1, a pressing rod 2, an adhesive tape 3, and an offset adjusting device 4, and the pressing rod 2 is arranged on the On the rack 1, the adhesive tape 3 is movably wound around the pressing rod 2, which is used to collect the sliced biological tissue samples, and the part where the pressing rod 2 contacts the adhesive tape 3 is arc-shaped to ensure that the adhesive tape is smoothly wound around the arc. On the shape part, the offset adjustment device 4 is connected with the pressure rod 2 to adjust the spatial posture of the pressure rod 2 in four degrees of freedom, that is, to eliminate the offset generated by the pressure rod 2 in the four degrees of freedom, that is, the opposite pressure can be realized. The control of the four degrees of freedom of the rod 2 is to realize the precise control of the distance between the tape 3 wound on the pressure rod 2 and the slice, and then the constant extrusion and adhesion, as much as possible to reduce the adhesion of the tape 3 during the extrusion process. deformation of the slices, while ensuring that the thickness of the collected slices is as consistent as possible.

Further, as shown in FIG. 1 and FIG. 2 , the offset adjustment device 4 includes a flexible adjustment mechanism 5 and a displacement actuator 6 , wherein the flexible adjustment mechanism 5 includes a first direction adjustment member 51 and a second direction adjustment member 52 , The displacement actuator 6 includes a first direction actuator 61 and a second direction actuator 62. The first direction adjustment member 51 cooperates with the first direction actuator 61 to eliminate the offset in the first direction, and the second direction The adjustment member 52 cooperates with the second direction actuator 62 to cancel the offset in the second direction.

Further, the first direction adjusting member 51 has the same structure as the second direction adjusting member 52, and the direction is vertical. As shown in FIG. 2, the first direction adjusting member 51 includes four parallel flexible boards that are nested inside and outside horizontally. The end of the flexible board is fixed on the frame 1 so that the flexible board can be displaced in the direction perpendicular to the board, but not in the direction parallel to the board. The second direction adjusting member 52 includes four parallel flexible boards that are nested inside and outside vertically. The ends of the flexible boards are fixed on the frame 1 so that the flexible boards are displaced in the direction parallel to the boards, and the flexible boards are displaced in the direction perpendicular to the boards. No displacement occurs in the direction of the plate.

Further, as shown in FIGS. 1 and 3 , the frame 1 includes a first support 7 and a second support 8 that are symmetrical to each other, and also includes a cutting module 9 , wherein the cutting module 9 includes a tool support rod 10 and a tool 11 , One end of the tool support rod 10 is fixed on the first support 7 and the other end is fixed on the second support 8 , and the tool 11 is arranged on the tool support rod 10 . During cutting, the cutter 11 is fixed with the cutter support 10, the biological tissue sample is placed under the cutting module 9, the biological tissue sample is moved, and the slices produced by cutting are adhered to the tape 3 to realize the collection of slices.

Further, the distance from the tangent line where the tape 3 is in contact with the pressing rod 2 and the cutter 11 is constant. The slicing moves with the tape 3 along the direction of the cutter 11, and the position of the pressing rod 2 around the tape 3 is controlled to keep the interval between the tape 3 and the upper surface of the cutter 11 constant, so that the upper surface of the cutter 11 does not align. The slices adhered to the adhesive tape 3 generate pressure, thereby realizing that the extrusion of the slices when the adhesive tape 3 adheres to the slices is not affected by other factors, so as to reduce the degree of deformation of the slices, while ensuring that the thicknesses of the slices collected by the adhesive tape 3 are consistent.

Further, the first support 7 and the second support 8 are provided with two first direction adjusting members 51 and second direction adjusting members 52 , one first direction actuator 61 and one second direction actuator 62 , wherein the two first direction adjusting members 51 and the second direction adjusting members 52 are respectively arranged symmetrically, and the end of the pressing rod 2 is arranged on the symmetrical points of the two first direction adjusting members 51 . Specifically, one end of the pressure rod 2 is connected to one end of the flexible plate on the first support 7, and the other end of the pressure rod 2 is connected to one end of the flexible plate on the second support 8. In fact, the offset adjustment device 4 is opposite to the pressure rod. 2 The control of the four degrees of freedom can be achieved by adjusting the two ends of the pressure rod 2. One end of the first direction actuator 61 abuts against the first direction adjustment member 51, and one end of the second direction actuator 62 abuts against the second direction adjustment member 52, so as to realize the first direction adjustment member 51 and the first direction actuation The actuator 61 cooperates to eliminate the offset in the first direction, and the second direction adjusting member 52 cooperates with the second direction actuator 62 to eliminate the offset in the second direction.

When adjusting the displacement of the pressing rod 2 in the horizontal direction, the first direction actuator 61 applies pressure to the flexible board arranged horizontally in the first direction adjusting member 51, so that the flexible board is displaced in the horizontal direction, thereby driving the pressing rod 2 Displacement in the horizontal direction. When adjusting the vertical displacement of the pressing rod 2, the second direction actuator 62 applies pressure to the flexible board vertically arranged in the second direction adjusting member 52, so that the flexible board is displaced in the vertical direction, thereby driving the pressing rod 2 produces displacement in the vertical direction. Thereby, the control of the four degrees of freedom of the pressing rod 2 is realized.

Among them, the four degrees of freedom refer to the two translational degrees of freedom a and b and the two rotational degrees of automation m and n as shown in FIG. 5 . Among them, O represents the center of mass of the pressure rod (because the two ends of the pressure rod are fixed, the pressure rod will not move along its axial direction, so the translational degree of freedom refers to the two non-axial displacement directions of the pressure rod). The displacement of the pressing rod can be converted into the displacement of the x-axis and the y-axis, so the offset generated by the pressing rod can be adjusted by an actuator parallel to these two directions.

In this embodiment, the offset adjustment device 4 is used to achieve nanoscale precision adjustment of the four degrees of freedom of the pressure rod 2. The principle of the flexible adjustment mechanism 5 is explained as follows: Fig. 4a is a structural diagram of the beam, one end of the beam is fixed, and the other end is Under the action of the external force F, the deformation displacement δ, ε and the deformation angle θ in the direction of the force will be generated; Figure 4b is the principle explanation diagram of the direction adjustment member, which is composed of a primary platform and a secondary platform. However, due to the existence of the secondary platform, the deformation displacement ε in the non-stressed direction of the primary platform during the stressing process can be eliminated, and then the platform deformation displacement can be moved along the stressing direction. It is based on this principle that the flexible adjustment mechanism 5 in the offset adjustment device 4 is designed to realize the control of the four degrees of freedom of the pressure rod.

Further, as shown in FIG. 1 , the end of the tool support rod 10 is detachably disposed on the first support 7 and the second support 8 through the fastening assembly, and the precise rolling angle of the tool 11 can be achieved through the fastening assembly. adjust.

Further, the fastening component includes a fixing block 12 and a nut 13. The fixing block 12 and the frame 1 are provided with grooves to accommodate the tool support rod 10, and the fixing block 12 and the frame 1 are provided with a nut 13 to match. screw holes.

Those skilled in the art can easily understand that the above are only preferred embodiments of the present invention, and are not intended to limit the present invention. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present invention, etc., All should be included within the protection scope of the present invention.

Claims (8)

1. A micro-contact slice collection device for thin sections of biological tissue, characterized in that, comprising a rack, a pressing rod, an adhesive tape, and an offset adjustment device, and the pressing rod is arranged on the rack, and the adhesive tape movably Winding around the pressing rod, the offset adjusting device is connected with the pressing rod and adjusts the spatial posture of the pressing rod in four degrees of freedom by adjusting the two ends of the pressing rod, wherein the four degrees of freedom are: Refers to 2 translational degrees of freedom and 2 rotational degrees of freedom; The offset adjustment device includes a flexible adjustment mechanism and a displacement actuator, the flexible adjustment mechanism includes a first direction adjustment member and a second direction adjustment member, and the displacement actuator includes a first direction actuator and a second direction adjustment member. A direction actuator, the first direction adjustment member cooperates with the first direction actuator to eliminate the offset of the first direction, and the second direction adjustment member cooperates with the second direction actuator to remove the offset in the second direction. 2 . The micro-contact slice collection device for thin sections of biological tissue according to claim 1 , wherein the first direction adjustment member has the same structure as the second direction adjustment member, and the direction is vertical, and the first direction adjustment member has the same structure. 3 . It includes four parallel flexible boards nested inside and outside, and the end of the flexible board is fixed on the frame so that the flexible board is displaced in the direction perpendicular to the board, and in the direction parallel to the board No displacement occurs. 3. The micro-contact slice collection device for thin sections of biological tissue according to claim 2, wherein the frame comprises a first support and a second support that are symmetrical with each other, and further comprises a cutting module, wherein the cutting The module includes a cutter support rod and a cutter, one end of the cutter support rod is fixed on the first support, the other end is fixed on the second support, and the cutter is arranged on the cutter support rod. 4 . The micro-contact slice collection device for thin sections of biological tissue according to claim 3 , wherein the distance from the tangent line where the tape is in contact with the pressing rod and the cutter is constant. 5 . 5 . The micro-contact slice collection device for thin sections of biological tissue according to claim 3 , wherein the first support and the second support are provided with one or more first direction adjustment members, second A direction adjusting member, a first direction actuator, and a second direction actuator. 6 . The micro-contact slice collection device for thin sections of biological tissue according to claim 3 , wherein the first support and the second support are provided with two first direction adjustment members and a second direction adjustment member. 7 . The adjusting piece, one first-direction actuator and one second-direction actuator, the two first-direction adjusting pieces and the second-direction adjusting piece are respectively arranged symmetrically, and the end of the pressing rod is arranged on the two On the symmetrical points of the first direction adjustment member or the second direction adjustment member, one end of the first direction actuator abuts against the first direction adjustment member, and one end of the second direction actuator abuts against the first direction adjustment member. the second direction adjustment member. 7 . The micro-contact slice collection device for thin sections of biological tissue according to claim 6 , wherein the end of the knife support rod is detachably arranged on the first support and the first support through a fastening assembly. 8 . on the second support. 8 . The micro-contact slice collection device for thin sections of biological tissue according to claim 7 , wherein the fastening component comprises a fixing block and a nut, and both the fixing block and the frame are provided with a part to accommodate the The groove of the tool support rod and the screw hole matched with the nut.

Images