CN110656029B

CN110656029B - Culture medium for adipose-derived stem cells

Info

Publication number: CN110656029B
Application number: CN201911107121.5A
Authority: CN
Inventors: 张鹏成
Original assignee: Anhui Kemen Biotechnology Co ltd
Current assignee: Anhui Kemen Biotechnology Co ltd
Priority date: 2019-11-13
Filing date: 2019-11-13
Publication date: 2023-01-24
Anticipated expiration: 2039-11-13
Also published as: CN110656029A

Abstract

The invention discloses a culture medium for adipose-derived stem cells, which comprises a culture frame, a culture dish, a limiting frame, a driving device, a sliding frame and a swinging device, wherein the culture dish is movably arranged on the culture frame, a slot is formed in the interior of the culture frame, the culture dish is movably clamped in the slot, the limiting frame and the driving device are arranged on the outer side of the culture frame, the driving device is movably connected with the limiting frame, and the driving device comprises a screw rod; the culture dish in the scheme is driven by the limiting frame and the driving device to move back and forth in the culture rack, so that the feeding and the stirring of materials in the culture dish are facilitated; pendulous device drives the second and changes board and puddler and carry out periodic rotation in the culture dish, compares with prior art, has improved the stirring scope to make the required material of culture medium stirring more even in the short time.

Description

Culture medium for adipose-derived stem cells

Technical Field

The invention relates to the technical field of culture media, in particular to a culture medium for adipose-derived stem cells.

Background

Adipose stem cells are a kind of stem cells having a multipotentiality, which have been isolated from adipose tissues in recent years. The method mainly recovers the repairing function of tissue cells, promotes the regeneration of cells, recovers the young face, simultaneously fully improves the body function, effectively improves diseases such as sub-health, premature senility and the like, and really and effectively resists aging from inside to outside, so that a culture medium required for preparing the adipose-derived stem cells is very critical; and the culture medium is in the preparation process, usually all mix required material together and stir, when the material is more, manual stirring will lead to mixing inhomogeneous, lead to part material still not to disperse easily, subsequent stem cell culture will lead to the nutrition to distribute unevenly, cause the survival rate to hang down, in addition, very inconvenient when the culture dish of stirring culture medium removes, need the manual work to carry, cause the pollution very easily, for this reason we provide a culture medium for adipose-derived stem cell, with the problem of proposing in solving above-mentioned background art.

Disclosure of Invention

The purpose of the invention can be realized by the following technical scheme: a culture medium for adipose-derived stem cells comprises a culture frame, a culture dish, a limiting frame, a driving device, a sliding frame and a swinging device, wherein the culture dish is movably arranged on the culture frame, a groove is formed in the culture frame, the culture dish is movably clamped in the groove, the limiting frame and the driving device are arranged on the outer side of the culture frame, the driving device is movably connected with the limiting frame, the driving device comprises a lead screw, the lower end of the swinging device is in threaded fit connection with the lead screw, when the culture dish is positioned in a popular science culture frame, the swinging device is positioned right above the culture dish and uniformly stirs the culture medium in the culture dish, symmetrically distributed support frames are arranged on the culture frame, symmetrically arranged limiting seats are arranged at the lower end of each support frame, the lead screw penetrates through the limiting seats, a connecting plate is arranged between the support frames, the upper end of the swinging device is arranged on the connecting plate, bearings are arranged on the parts, and are in contact with the lead screw, and are rotatably connected with the limiting seats, so that the lead screw can conveniently rotate;

a connecting rod is arranged on the outer side of the culture dish, and a push rod is arranged at the end part of the connecting rod; an elastic piece is arranged in the limiting frame, a movable block is arranged at the end part of the elastic piece, an insertion block is welded on one side of the movable block, the insertion block penetrates through the limiting frame, an insertion groove is formed in the limiting frame, the end part of the insertion block penetrates through the insertion groove, and the end part of the insertion block is connected with the tail end of the push rod, so that the movable block can drive the culture dish to move; the driving device further comprises a first motor, a driving gear, a driven gear and a winding roller, wherein the driving gear is rotated at the output end of the first motor, the driving gear is meshed with the driven gear, the driven gear is vertically connected with one end of a screw rod, the winding roller is arranged at the tail end of the screw rod, and a winding belt penetrates through a limiting frame and is wound on the winding roller; therefore, the driving gear is driven to rotate by driving the first motor, so that the winding roller is driven to rotate clockwise, the coiling belt is wound on the winding roller, the culture dish moves outwards along the culture rack at the moment, and otherwise, the culture dish moves inwards the culture rack;

the sliding frame is arranged between the two supporting plates; the swinging device comprises a second motor, a swinging rod, a first rotating plate, a sliding plate, a threaded sleeve, a second rotating plate and a stirring rod, the second motor is arranged on a connecting plate, the output end of the second motor is rotated and provided with the swinging rod, the first motor and the second motor are synchronously started, so that the first motor drives the screw rod to rotate when working, the second motor works and drives the swinging rod to rotate, the swinging rod is located below the connecting plate, the end part of the swinging rod is provided with the first rotating plate, the tail end of the first rotating plate is vertically provided with an insertion rod, the insertion rod penetrates through the sliding plate, the sliding plate is slidably arranged on the sliding frame, the end part of the sliding plate is provided with the threaded sleeve, the screw rod threads penetrate through the threaded sleeve, therefore, under the rotation of the screw rod, the threaded sleeve moves back and forth along the screw rod to drive the sliding plate to move, the tail end of the insertion rod is provided with the second rotating plate, the lower surface of the second rotating plate is provided with the stirring rods distributed in an array, and the stirring rod can stir the culture medium on the culture dish.

Preferably, the spout has been seted up to the inboard of culture shelf, and the lower surface one end of culture dish is equipped with the pulley, makes things convenient for the culture dish to remove, and the lower surface middle part of culture dish is equipped with symmetric distribution's slider, and the slider slides and sets up in the spout, stability when improving the culture dish round trip movement.

Preferably, the outside of culture rack is equipped with fixed block and guide arm respectively, and the second motor setting is on the fixed block, and the guide arm is located one side of spacing, and the guide arm is walked around to the one end of winding, gets into to wind-up roll rolling by smooth guide arm when making the winding come out, avoids winding and spacing direct contact to cause the friction and reduce the life-span.

Preferably, the upper end welding of cultivateing the frame has the backup pad, is equipped with the activity groove between backup pad and the cultivation frame, and the one end and the backup pad of support frame are connected perpendicularly, and the connecting rod setting is at the middle part of culture dish, therefore when the culture dish was located the cultivation frame completely, the tank bottom of establishing the activity groove was inserted to the connecting rod.

Preferably, the connecting block has all been welded at the both ends of balladeur train, and the upper end of connecting block is connected with the lower surface of backup pad, and the wearing groove has been seted up to the inside of balladeur train, and the inserted bar runs through the wearing groove and wears out from the other end of slide.

Preferably, the cross section of the sliding plate is U-shaped, the sliding plate is movably clamped on the sliding frame, and under the limitation of the inserted link and the threaded sleeve, the sliding plate can only move back and forth along the sliding frame, so that the sliding plate is prevented from being separated from the sliding frame.

Preferably, one end of the second rotating plate is flush with one end of the first rotating plate, so that the blockage of stirring caused by overlong second rotating plate is avoided.

Preferably, one side of movable block is equipped with the cutting ferrule, the spacing groove that the cross-section is "T" shape is seted up to the inside of cutting ferrule, the tip of elastic component is equipped with the cardboard, the cardboard can be dismantled the card and establish in the spacing groove of cutting ferrule, when putting into culture medium to the culture dish in, establish the cardboard card on the cutting ferrule, the winding can pull the movable block and remove this moment, and shift out the culture dish, when stirring the culture medium, take out the cardboard from the cutting ferrule, the movable block can not form the restraint to the winding this moment, thereby the position of culture dish can not change.

The invention has the beneficial effects that:

1. the culture dish in the scheme is driven by the limiting frame and the driving device to move back and forth in the culture rack, so that the feeding and the stirring of materials in the culture dish are facilitated;

2. pendulous device drives the second and changes board and puddler and carry out periodic rotation in the culture dish, compares with prior art, has improved the stirring scope to make the required material of culture medium stirring more even in the short time.

Drawings

The invention will be further described with reference to the accompanying drawings.

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of a culture shelf of the present invention;

FIG. 3 is a schematic view of a removable culture dish according to the present invention;

FIG. 4 is a schematic view of a swing apparatus of the present invention;

fig. 5 is a schematic view of the driving device of the present invention.

In the figure: the culture device comprises a culture frame 1, a support plate 11, a sliding chute 12, a support frame 13, a limiting seat 131, a connecting plate 14, a fixed block 15, a guide rod 16, a culture dish 2, a pulley 21, a connecting rod 22, a push rod 23, a sliding block 24, a limiting frame 3, an elastic part 31, a clamping plate 311, a movable block 32, an insertion block 321, a clamping sleeve 322, a winding belt 33, a driving device 4, a first motor 41, a driving gear 42, a driven gear 43, a winding roller 44, a screw rod 45, a sliding frame 5, a connecting block 51, a groove 52 penetrating device 6, a second motor 61, a swing rod 62, a first rotating plate 63, an insertion rod 631, a sliding plate 64, a screw sleeve 641, a second rotating plate 65 and a stirring rod 66.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-5, the present invention provides a technical solution: a culture medium for adipose-derived stem cells comprises a culture frame 1, a culture dish 2, a limiting frame 3, a driving device 4, a sliding frame 5 and a swinging device 6, wherein the culture dish 2 is movably arranged on the culture frame 1, a groove is formed in the culture frame 1, the culture dish 2 is movably clamped in the groove, the limiting frame 3 and the driving device 4 are arranged on the outer side of the culture frame 1, the driving device 4 is movably connected with the limiting frame 3, the driving device 4 comprises a lead screw 45, the lower end of the swinging device 6 is in threaded fit connection with the lead screw 45, when the culture dish 2 is positioned in the popular culture frame 1, the swinging device 6 is positioned right above the culture dish 2 and uniformly stirs the culture medium in the culture dish 2, symmetrically distributed support frames 13 are arranged on the culture frame 1, symmetrically arranged limiting seats 131 are arranged at the lower end of each support frame 13, the lead screw 45 penetrates through the limiting seats 131, a connecting plate 14 is arranged between the support frames 13, the upper end of each swinging device 6 is arranged on the connecting plate 14, a part, where the limiting seat 131 is in contact with each support frame 45, is provided with a bearing, and rotatably connected with the limiting seat 131, so that the lead screw 45 can conveniently rotate;

as shown in fig. 3 and 5, a connecting rod 22 is arranged on the outer side of the culture dish 2, and a push rod 23 is arranged at the end of the connecting rod 22; the inner part of the limiting frame 3 is provided with an elastic part 31, the end part of the elastic part 31 is provided with a movable block 32, one side of the movable block 32 is welded with an inserting block 321, the inserting block 321 penetrates through the limiting frame 3, a slot is formed in the limiting frame 3, the end part of the inserting block 321 penetrates through the slot, and the end part of the inserting block is connected with the tail end of the push rod 23, so that the movable block 32 can drive the culture dish 2 to move, when the elastic part 31 contracts, the culture dish 2 is positioned at the outer side of the culture frame 1, when the elastic part 31 stretches, the culture frame 1 is positioned at the inner side of the culture frame 1, and the other side of the movable block 32 is provided with a coiling belt 33; the driving device 4 further comprises a first motor 41, a driving gear 42, a driven gear 43 and a winding roller 44, the driving gear 42 is rotated at the output end of the first motor 41, the driving gear 42 is meshed with the driven gear 43, the driven gear 43 is vertically connected with one end of a screw rod 45, the winding roller 44 is arranged at the tail end of the screw rod 45, and the winding belt 33 passes through the limiting frame 3 and is wound on the winding roller 44; therefore, the first motor 41 can be driven to drive the driving gear 42 to rotate, so as to drive the winding roller 44 to rotate clockwise, and wind the coiling tape 33 on the winding roller 44, at this time, the culture dish 2 moves outwards along the culture rack 1, otherwise, the culture dish moves inwards the culture rack 1;

as shown in fig. 1 and 4, the carriage 5 is disposed between the two support plates 11; the swinging device 6 comprises a second motor 61, a swinging rod 62, a first rotating plate 63, a sliding plate 64, a screw sleeve 641, a second rotating plate 65 and a stirring rod 66, the second motor 61 is arranged on the connecting plate 14, the output end of the second motor 61 is rotatably provided with the swinging rod 62, the first motor 41 and the second motor 61 are synchronously started, so when the first motor 41 works, the screw rod 45 is driven to rotate, the second motor 61 works and drives the swinging rod 62 to rotate, the swinging rod 62 is positioned below the connecting plate 14, the end part of the swinging rod 62 is provided with the first rotating plate 63, the maximum length of the swinging rod 62 and the first rotating plate 63 is less than half of the length of the sliding frame 5, the tail end of the first rotating plate 63 is vertically provided with an inserting rod 631, the inserting rod 631 penetrates through the sliding plate 64, the sliding plate 64 is arranged on the sliding frame 5 in a sliding manner, the end part of the sliding plate 64 is provided with the screw sleeve, the screw sleeve 45 penetrates through the screw sleeve, so that under the rotation of the screw rod 45, the screw sleeve 641 moves back and forth along the screw rod 45 to drive the screw rod 641 to move, the second rotating plate 631, the tail end of the sliding plate 631 is provided with the second rotating plate 65, the stirring rod 66 distributed on the array of the second rotating plate 65, and the stirring rod 66 can stir the culture medium 641.

As shown in fig. 2 and 3, the inner side of the culture rack 1 is provided with a sliding groove 12, one end of the lower surface of the culture dish 2 is provided with a pulley 21, the culture dish 2 is convenient to move, the middle part of the lower surface of the culture dish 2 is provided with symmetrically distributed sliding blocks 24, the sliding blocks 24 are arranged in the sliding groove 12 in a sliding manner, and the stability of the culture dish 2 during the back and forth movement is improved.

As shown in fig. 1 and 5, the outer side of the culture shelf 1 is provided with a fixing block 15 and a guide rod 16, the second motor 61 is arranged on the fixing block 15, the guide rod 16 is located at one side of the limiting shelf 3, and one end of the tape 33 bypasses the guide rod 16, so that the tape 33 enters the wind-up roller 44 from the smooth guide rod 16 to be wound when coming out, thereby avoiding the reduction of service life caused by friction due to direct contact between the tape 33 and the limiting shelf 3.

As shown in FIG. 1, a support plate 11 is welded to the upper end of the culture shelf 1, a movable groove is formed between the support plate 11 and the culture shelf 1, one end of a support frame 13 is vertically connected with the support plate 11, and a connecting rod 22 is arranged in the middle of the culture dish 2, so that when the culture dish 2 is completely positioned in the culture shelf 1, the connecting rod 22 is inserted into the bottom of the movable groove.

As shown in fig. 4, the connecting blocks 51 are welded at both ends of the carriage 5, the upper end of the connecting block 51 is connected to the lower surface of the supporting plate 11, the through groove 52 is opened in the carriage 5, and the inserting rod 631 penetrates through the through groove 52 and penetrates out from the other end of the sliding plate 64.

As shown in fig. 4, the slide plate 64 has a U-shaped cross section, and the slide plate 64 is movably engaged with the carriage 5 and can only move back and forth along the carriage 5 under the restriction of the insertion rod 631 and the screw 641, so as to avoid being separated from the carriage 5.

As shown in fig. 4, one end of the second rotating plate 65 is flush with one end of the first rotating plate 63, so as to avoid the blockage of stirring caused by the overlong second rotating plate 65.

As shown in fig. 5, one side of movable block 32 is equipped with cutting ferrule 322, the spacing groove that the cross-section is "T" shape is seted up to cutting ferrule 322's inside, the tip of elastic component 31 is equipped with cardboard 311, cardboard 311 can dismantle the card and establish in cutting ferrule 322's spacing groove, when putting into culture medium to culture dish 2, establish cardboard 311 card on cutting ferrule 322, winding 33 can pull movable block 32 and remove this moment, and shift out culture dish 2, when stirring culture medium, take out cardboard 311 from cutting ferrule 322, movable block 32 can not form the restraint to winding 33 this moment, thereby culture dish 2's position can not change.

When the culture medium is used, when various components and materials required by the culture medium are poured onto the culture dish 2, the first motor 41 is started, the first motor 41 drives the driving gear 42 to rotate, the driving gear 42 drives the driven gear 43 to rotate, so that the winding roller 44 arranged on one side of the driving gear is driven to rotate, the winding belt 33 is wound on the winding roller 44, the movable block 32 pushes the push rod 23 to move, the culture dish 2 is driven to move out of the culture rack 1, and material paving is facilitated; after, under the elasticity of elastic component 31, bring culture dish 2 into culture rack 1, open first motor 41 and second motor 61 simultaneously this moment, first motor 41 drives lead screw 45 and rotates, and lead screw 45 drives slide 64 and removes, and second motor 61 drives first commentaries on classics board 63 and second commentaries on classics board 65 and rotates simultaneously for second commentaries on classics board 65 can be periodic motion on culture dish 2, conveniently obtains the culture medium with its inside material misce bene.

In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

Claims

1. A culture medium for adipose-derived stem cells comprises a culture frame (1), a culture dish (2), a limiting frame (3), a driving device (4), a sliding frame (5) and a swinging device (6), and is characterized in that the culture dish (2) is movably arranged on the culture frame (1), the limiting frame (3) and the driving device (4) are arranged on the outer side of the culture frame (1), the driving device (4) is movably connected with the limiting frame (3), the driving device (4) comprises a lead screw (45), the lower end of the swinging device (6) is in threaded fit connection with the lead screw (45), symmetrically distributed support frames (13) are arranged on the culture frame (1), limiting seats (131) are symmetrically arranged at the lower ends of the support frames (13), the lead screw (45) penetrates through the limiting seats (131), a connecting plate (14) is arranged between the support frames (13), and the upper end of the swinging device (6) is arranged on the connecting plate (14);

a connecting rod (22) is arranged on the outer side of the culture dish (2), and a push rod (23) is arranged at the end part of the connecting rod (22); an elastic piece (31) is arranged in the limiting frame (3), a movable block (32) is arranged at the end part of the elastic piece (31), an insertion block (321) is welded at one side of the movable block (32), the insertion block (321) penetrates through the limiting frame (3), the end part of the insertion block is connected with the tail end of the push rod (23), and a winding belt (33) is arranged at the other side of the movable block (32); the driving device (4) further comprises a first motor (41), a driving gear (42), a driven gear (43) and a winding roller (44), the driving gear (42) is rotated at the output end of the first motor (41), the driving gear (42) is meshed with the driven gear (43) to be connected, the driven gear (43) is vertically connected with one end of a screw rod (45), the winding roller (44) is arranged at the tail end of the screw rod (45), and the winding tape (33) penetrates through the limiting frame (3) and is wound on the winding roller (44);

the sliding frame (5) is arranged between the two supporting plates (11); swing device (6) include second motor (61), pendulum rod (62), first commentaries on classics board (63), slide (64), swivel nut (641), second commentaries on classics board (65) and puddler (66), second motor (61) set up on connecting plate (14), the tip that the output rotation of second motor (61) was equipped with pendulum rod (62) is equipped with first commentaries on classics board (63), there is inserted bar (631) perpendicularly at the end of first commentaries on classics board (63), slide (64) are run through slide (64), slide (64) slide and set up on balladeur train (5), the tip of slide (64) is equipped with swivel nut (641), screw rod (45) screw thread runs through swivel nut (641), the end of inserted bar (631) is equipped with second commentaries on classics board (65), the lower surface of second commentaries on classics board (65) is equipped with puddler (66) that the array distributes.

2. The culture medium for the adipose-derived stem cells according to claim 1, wherein a sliding groove (12) is formed in the inner side of the culture frame (1), a pulley (21) is arranged at one end of the lower surface of the culture dish (2), symmetrically distributed sliding blocks (24) are arranged in the middle of the lower surface of the culture dish (2), and the sliding blocks (24) are slidably arranged in the sliding groove (12).

3. The adipose-derived stem cell culture medium according to claim 1, wherein the culture rack (1) is provided with a fixing block (15) and a guide rod (16) at the outer side thereof, the second motor (61) is arranged on the fixing block (15), the guide rod (16) is arranged at one side of the limiting rack (3), and one end of the tape (33) bypasses the guide rod (16).

4. The culture medium for the adipose-derived stem cells, according to the claim 1, is characterized in that a support plate (11) is welded at the upper end of the culture frame (1), a movable groove is arranged between the support plate (11) and the culture frame (1), one end of the support frame (13) is vertically connected with the support plate (11), and the connecting rod (22) is inserted at the bottom of the movable groove.

5. The adipose-derived stem cell culture medium according to claim 1, wherein the two ends of the carriage (5) are welded with connecting blocks (51), the upper ends of the connecting blocks (51) are connected with the lower surface of the supporting plate (11), a through groove (52) is formed inside the carriage (5), and the inserting rod (631) penetrates through the through groove (52) and penetrates out from the other end of the sliding plate (64).

6. The adipose-derived stem cell culture medium of claim 1, wherein the cross-section of the sliding plate (64) is in a U shape, and the sliding plate (64) is movably clamped on the sliding frame (5).

7. The adipose-derived stem cell culture medium according to claim 1, wherein one end of the second rotating plate (65) is flush with one end of the first rotating plate (63).

8. The culture medium for the adipose-derived stem cells as claimed in claim 1, wherein a cutting sleeve (322) is arranged on one side of the movable block (32), a limiting groove with a T-shaped cross section is formed inside the cutting sleeve (322), a clamping plate (311) is arranged at the end of the elastic member (31), and the clamping plate (311) is detachably clamped in the limiting groove of the cutting sleeve (322).