CN110655556A - Preparation and method of immunoregulatory peptide - Google Patents
Preparation and method of immunoregulatory peptide Download PDFInfo
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Abstract
The invention provides a method for separating and purifying immunoregulatory peptide by utilizing radix pseudostellariae, which takes radix pseudostellariae as a raw material, and obtains specific immune polypeptide through protein extraction, pepsin and trypsin double-step enzymolysis, separation, purification and freeze drying, wherein the molecular weight of the specific immune polypeptide is 522 Da, and the amino acid complete sequence is as follows: Arg-Gly-Pro-Pro-Pro. The invention eliminates the defects of the immunoregulation medicament, eliminates the public worry about the artificial immunoregulator, and lays a theoretical foundation for developing the food-source-based immune polypeptide and exploring the wide application of the food-source-based immune polypeptide in food and medicine.
Description
Technical Field
The invention relates to a method for separating and purifying immunoregulatory peptide by utilizing radix pseudostellariae, belonging to the technical field of biology.
Background
Immunity is a specific physiological response that occurs when the body is exposed to "antigenic foreign bodies" or "isohexides," the main objective of which is to maintain homeostasis in the body. The immune system responsible for the immune function of the body is a network of cells, tissues and organs that eliminate potentially harmful substances such as bacteria, viruses, fungi, protozoa and prevent the growth of cancer cells. In the living of the body, the immune system serves as the first line of defense against pathogens, providing protection before bodily functions are impaired. However, the immune system is also affected by many factors, including stress, unhealthy lifestyle, pathogens and antigens that can destroy the body's immune system. Therefore, drugs for regulating the immune response of human body have come into play, such as cyclosporine, tacrolimus, glucocorticoid, phytol, aristolochic acid, graphene, levamisole and the like, which have been successfully applied to the regulation of the immune response of human body. However, the toxic side effects and high cost of these drugs limit their use in patients, and most immunomodulatory drugs are not suitable for chronic or prophylactic use. Therefore, the discovery of novel immunologically active peptides from food proteins is an effective therapeutic and prophylactic approach.
Radix pseudostellariae is the root tuber of the pseudostellaria heterophylla of the dianaceae family, also known as the root tuber of the juvenile sea cucumber, namely the juvenile sea cucumber, the root tuber of the pseudostellaria heterophylla, the root tuber of the rice-leaved ginseng and the like, since the beginning of the Qing Dynasty, the herbal of the New Japan, the herbal of the Japan, the Chinese medical works are recorded. The main planting areas of the radix pseudostellariae comprise Fujian, Jiangsu, Shandong, Anhui and the like, wherein the Fujian Cudrania tricuspidata can be planted from the end of Qing dynasty, and is famous at home and abroad and has the reputation of the country of the radix pseudostellariae. So far, researches on chemical components and medicinal effects of radix pseudostellariae are quite extensive, but no relevant reports on preparation of polypeptides by proteolysis of radix pseudostellariae exist.
Therefore, the invention prepares the high-efficiency immune polypeptide with a specific amino acid sequence from the radix pseudostellariae protein zymolyte. The polypeptide can be applied to the industries of food and health care products.
Disclosure of Invention
In order to solve the problems, the invention provides a method for separating and purifying the immunoregulatory peptide by utilizing radix pseudostellariae, and the polypeptide obtained by the method has a specific amino acid sequence.
In order to realize the purpose, the following technical scheme is adopted:
the amino acid sequence of the immunoregulatory peptide is Arg-Gly-Pro-Pro-Pro (RGPPP), and the preparation method comprises the following steps:
(1) extracting the protein of the radix pseudostellariae: grinding radix Pseudostellariae into powder, and extracting radix Pseudostellariae protein by alkali extraction and acid precipitation;
(2) preparation of radix pseudostellariae protein zymolyte: carrying out double-step enzymolysis on the radix pseudostellariae protein by utilizing pepsin and trypsin to obtain a radix pseudostellariae protein zymolyte;
(3) separating and purifying the radix pseudostellariae protein zymolyte: separating and purifying radix Pseudostellariae protein zymolyte by Sephadex G25 chromatography and RP-HPLC reversed-phase high performance liquid chromatography, collecting fraction with highest immunological activity, freeze drying, and identifying amino acid sequence of fraction by Nano LC-MS/MS.
The radix pseudostellariae protein in the step (1) is extracted by grinding radix pseudostellariae into powder, adding 0.2 ~ 0.3.3 mol/L sodium hydroxide solution (NaOH) with the corresponding volume according to the mass concentration of 4.0 ~ 5.0.0% of the radix pseudostellariae powder, leaching for 1.0 ~ 3.0.0 h in a water bath at 50 ~ 60 ℃, centrifuging to collect supernatant after leaching, adjusting the pH value to 2.5 ~ 3.5.5 by using 1 mol/L hydrochloric acid (HCl), standing for 1 ~ 3 h, centrifuging to collect precipitate, and freeze-drying to obtain the radix pseudostellariae protein.
The preparation method of the radix pseudostellariae protein zymolyte in the step (2) comprises the following steps of enabling the concentration of the radix pseudostellariae protein to be 1.0 ~ 2.0.0 w/v%, enabling the pH to be 1.5 ~ 2.5.5, enabling the temperature to be 30 ~ 40 ℃, enabling the enzymolysis time to be 4.0 ~ 6.0.0 h, adding 8.0 ~ 10.0.0 w/w% of pepsin for enzymolysis, adjusting the pH to be 6.5 ~ 7.5.5 after the reaction is finished, adding 8.0 ~ 10.0.0 w/w% of trypsin, enabling the enzymolysis time to be 2.0 ~ 4.0.0 h, immediately placing the mixture in a boiling water bath for boiling for 10 ~ 20 min after the reaction is finished, stopping the reaction, centrifuging the reacted solution, collecting supernatant, and freeze-drying to obtain the radix pseudostellariae protein zymolyte product.
The specific operation of the separation and purification of the radix pseudostellariae protein zymolyte in the step (3) is that the radix pseudostellariae protein zymolyte liquid in the step (2) is separated and purified, sephadex G25 takes deionized water as eluent, the sample loading amount is 5mL, the flow rate is 0.3 mL/min, the detection wavelength is 214nm, the immunocompetence of the eluent corresponding to each absorption peak is determined, the component with the highest immunocompetence is collected to be subjected to reversed-phase high-performance liquid chromatography separation, the reversed-phase high-performance liquid chromatography separation takes acetonitrile solution with the concentration gradient of 0 ~ 55 v/v% and containing trifluoroacetic acid with the volume fraction of 0.05% as eluent to be subjected to linear elution, the used chromatographic column is Gemini 5 mu C18, the sample loading amount is 100 mu L, the flow rate is 1 mL/min, the detection wavelength is 214nm, the immunocompetence of the eluent corresponding to each absorption peak is determined, the component with the highest immunocompetence is collected to be freeze-dried, the amino acid sequence of the component is identified by NaLC-MS/MS, and the amino acid sequence of the Pro-Pro of the amino acid of the peptide of the amino acid adjusting peptide of the Pro-Pro of the.
The specific operation of the determination of the immunological activity in the step (3) is as follows: the immunological activity was evaluated as the activity of promoting the proliferation of splenic lymphocytes in mice in vitro. The mouse spleen lymphocyte suspension is added into a 96-well plate, phosphate buffer is used as a blank control, and the final concentration of the sample is 10 mug/mL, 50 mug/mL and 100 mug/mL respectively. After adding the sample, the mixture is placed in 5% CO at 37 DEG C2After culturing in an incubator for different times, adding 20 mu L of 3- (4, 5-dimethylthiazole-2) -2, 5-diphenyl tetrazolium bromide (5 mg/mL) into each hole, continuing culturing for 4 h, centrifuging, removing supernatant, adding 200 mu L of dimethyl sulfoxide, shaking at low speed for 10 min, and detecting the light absorption value at 570 nm.
The invention has the beneficial effects that: the invention aims at overcoming the defects of toxic and side effects, high cost and the like of the existing immunoregulation medicaments, aims at searching a natural immunoregulator, takes the ginseng as a starting point, focuses on the process control of the two-step enzymolysis of pepsin and trypsin, and prepares active polypeptide with specific peptide chain length, so that the immunocompetence is realized efficiently. The enzymolysis technology adopted by the invention is simple and efficient, the activity of the enzymolysis product can be tracked so as to achieve directional enzyme digestion, the cost waste is avoided, the obtained polypeptide has immunological activity, and a theoretical basis is provided for the application of the polypeptide in food and health care product industries.
Drawings
FIG. 1 shows the elution pattern (A) of G25 of the protein zymolyte of radix Pseudostellariae and the immunological activity (B) of each component.
FIG. 2 shows the elution profile of RP-HPLC (A) and the immunoreactivity of each fraction (B).
Fig. 3 is a F18 total ion flow diagram.
FIG. 4 is a peptide fingerprint of an immunomodulatory peptide.
FIG. 5 is a graph showing the proliferative activity of immunomodulatory peptides.
Detailed Description
Example 1
The amino acid sequence of the immunoregulation peptide is Arg-Gly-Pro-Pro-Pro.
The preparation method comprises the following steps:
the first step is as follows: pulverizing radix Pseudostellariae into powder with a traditional Chinese medicine pulverizer, and sieving with a 60-mesh sieve; weighing 5.0 g of radix pseudostellariae powder, adding 105 mL of 0.27 mol/L sodium hydroxide solution, leaching for 1 h at 52.1 ℃, centrifuging at 10,000 rpm for 20 min, taking supernatant, adjusting pH to 3.0 by using 1 mol/L hydrochloric acid, standing for 1 h, centrifuging at 5,000 rpm for 20 min, taking precipitate, and freeze-drying to obtain the radix pseudostellariae protein.
The second step is that: weighing 10.0 g of radix pseudostellariae protein, adding 100 mL of deionized water, adjusting the pH value to 2.0, adding 0.8 g of pepsin, placing in a boiling water bath for boiling for 10 min to terminate the reaction after carrying out enzymolysis for 5.0 h at 37 ℃, adjusting the pH value to 7.0, adding 0.8 g of trypsin, placing in a boiling water bath for enzymolysis for 3.0 h, centrifuging for 20 min at 10,000 rpm, taking the supernatant, and freeze-drying to obtain the radix pseudostellariae protein hydrolysate.
The third step: weighing 100 mg of radix pseudostellariae protein zymolyte, adding 5mL of deionized water, stirring until the radix pseudostellariae protein zymolyte is completely dissolved, centrifuging at 8,000 rpm for 20 min, taking supernatant, filtering through a 0.22 mu m filter membrane, and separating and purifying by utilizing sephadex G25: using deionized water as eluent, flow rate of 0.3 mL/min, detection wavelength of 214nm, measuring the immunocompetence of eluent corresponding to each absorption peak, and measuring the elution curve (FIG. 1A) and immunocompetence of each obtained peak (FIG. 1B).
And fourthly, collecting the component G3 with the highest immunocompetence for carrying out reversed-phase high-performance liquid chromatography separation, namely weighing 3200 mg of G, adding 5mL of deionized water, fully dissolving the G, then passing through a 0.22-micron filter membrane, and loading the sample in an amount of 100 mu L, wherein in the reversed-phase high-performance liquid chromatography separation, acetonitrile solution with a concentration gradient of 0 ~ 55 v/v% and containing trifluoroacetic acid with a volume fraction of 0.05% is used as eluent for linear elution, a chromatographic column is Gemini 5 mu C18, the flow rate is 1 mL/min, the detection wavelength is 214nm, the immunocompetence of the eluent corresponding to each absorption peak is determined, and the elution curve (figure 2A) and the immunocompetence (figure 2B) of each obtained peak are determined.
The fifth step: the fraction F18 with the highest immunocompetence was collected, and after freeze-drying, the amino acid sequences of the fractions were identified by Nano LC-MS/MS. The amino acid sequence of the component is identified by using Nano LC-MS/MS, the total ion flow diagram is shown in figure 3, and then the ion peak of 77.35 min is identified by using MS/MS, so that the amino acid complete sequence is obtained (shown in figure 4): Arg-Gly-Pro-Pro-Pro (molecular weight 522 Da) is the immunoregulation peptide.
And a sixth step: the immunological activity was evaluated as the activity of promoting the proliferation of splenic lymphocytes in mice in vitro. The mouse spleen lymphocyte suspension is added into a 96-well plate, phosphate buffer is used as a blank control, and the final concentration of the sample is 10 mug/mL, 50 mug/mL and 100 mug/mL respectively. After adding the sample, the mixture is placed in 5% CO at 37 DEG C2After culturing in an incubator for different times, adding 20 mu L of 3- (4, 5-dimethylthiazole-2) -2, 5-diphenyl tetrazolium bromide (5 mg/mL) into each hole, continuing culturing for 4 h, centrifuging, removing supernatant, adding 200 mu L of dimethyl sulfoxide, shaking at low speed for 10 min, and detecting the light absorption value at 570 nm. The immunomodulatory peptides of the invention reached a maximum spleen cell stimulation index of 1.25 at a stimulation time of 48h at a concentration of 100. mu.g/mL (FIG. 5).
The above description is only a preferred embodiment of the present invention, and all equivalent changes and modifications made in accordance with the claims of the present invention should be covered by the present invention.
SEQUENCE LISTING
<110> Fuzhou university
<120> preparation and method of immunoregulatory peptide
<130> 1
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 5
<212> PRT
<213> Artificial sequence
<400> 1
Arg Gly Pro Pro Pro
1 5
Claims (5)
1. An immunomodulatory peptide, comprising: the amino acid sequence of the immunity polypeptide is Arg-Gly-Pro-Pro-Pro.
2. A method of preparing an immunomodulatory peptide of claim 1, wherein: the method comprises the steps of taking radix pseudostellariae as a raw material, extracting protein, carrying out enzymolysis on the protein, separating, purifying, freezing and drying to obtain the immune polypeptide.
3. The preparation method of the immunoregulatory peptide according to claim 2, wherein the specific steps of protein extraction are grinding radix pseudostellariae into powder, adding 0.2 ~ 0.3.3 mol/L sodium hydroxide solution according to the mass concentration of 4.0 ~ 5.0.0% of the radix pseudostellariae powder, then leaching for 1.0 ~ 3.0.0 h at 50 ~ 60 ℃, centrifuging after leaching, collecting supernatant, adjusting the pH value of the supernatant to 2.5 ~ 3.5.5 by using 1 mol/L hydrochloric acid, settling for 1 ~ 3 h, centrifuging, and freeze-drying to obtain the radix pseudostellariae protein.
4. The preparation method of the immunomodulatory peptide of claim 2, wherein the enzymolysis comprises the steps of hydrolyzing radix Pseudostellariae protein at 1.0 ~ 2.0.0 w/v%, pH 1.5 ~ 2.5.5, temperature 30 ~ 40 ℃, enzymolysis time 4.0 ~ 6.0.0 h, adding 8.0 ~ 10.0.0 w/w% pepsin, adjusting pH to 6.5 ~ 7.5.5, adding 8.0 ~ 10.0.0 w/w% trypsin for 2.0 ~ 4.0.0 h, boiling in boiling water bath for 10 ~ 20 min immediately after the reaction is finished, terminating the reaction, centrifuging and collecting supernatant to obtain immunomodulatory peptidase hydrolysis product.
5. The method for preparing immunomodulatory peptide of claim 2, wherein the specific steps of separation and purification include separating enzymatic hydrolysate by Sephadex G25 chromatography and RP-HPLC reversed-phase HPLC, monitoring immunocompetence of separated components, collecting components with optimal immunocompetence, separating Sephadex G25 with deionized water as eluent, loading 5mL of the eluent, flow rate of 0.3 mL/min, detection wavelength of 214nm, determining immunocompetence of eluent corresponding to each absorption peak, collecting components with highest immunocompetence, performing reversed-phase HPLC, performing linear elution by acetonitrile solution containing 0.05% trifluoroacetic acid by volume fraction and having concentration gradient of 0 ~ 55 v/v%, using Gemini 5 μ C18 as chromatographic column, loading 100 μ L of the eluent, flow rate of 1 mL/min, detection wavelength of 214nm, determining immunocompetence of eluent corresponding to each absorption peak, collecting components with highest immunocompetence, and identifying amino acid sequence of NaMS-no LC after freeze drying.
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| PCT/CN2020/073062 WO2021082294A1 (en) | 2019-10-31 | 2020-01-19 | Preparation of and method for immunomodulatory peptide |
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Cited By (5)
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| CN111825745A (en) * | 2020-07-29 | 2020-10-27 | 中国科学院华南植物园 | Immunoregulatory active heptapeptide and preparation method and application thereof |
| CN111978374A (en) * | 2020-07-29 | 2020-11-24 | 中国科学院华南植物园 | Immunoregulation active hexapeptide and preparation method and application thereof |
| WO2021082294A1 (en) * | 2019-10-31 | 2021-05-06 | 福州大学 | Preparation of and method for immunomodulatory peptide |
| CN114195860A (en) * | 2021-12-16 | 2022-03-18 | 吉林国瑞医药有限公司 | Anti-angiogenesis and anti-tumor active peptide from codonopsis pilosula thunb, and preparation method and application thereof |
| CN114213516A (en) * | 2021-12-16 | 2022-03-22 | 吉林国瑞医药有限公司 | Codonopsis lanceolata-derived lactagogue and breast tumor-resistant active peptide, and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110655556B (en) * | 2019-10-31 | 2021-05-18 | 福州大学 | Preparation and method of immunoregulatory peptide |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021082294A1 (en) * | 2019-10-31 | 2021-05-06 | 福州大学 | Preparation of and method for immunomodulatory peptide |
| CN111825745A (en) * | 2020-07-29 | 2020-10-27 | 中国科学院华南植物园 | Immunoregulatory active heptapeptide and preparation method and application thereof |
| CN111978374A (en) * | 2020-07-29 | 2020-11-24 | 中国科学院华南植物园 | Immunoregulation active hexapeptide and preparation method and application thereof |
| CN111825745B (en) * | 2020-07-29 | 2022-03-04 | 中国科学院华南植物园 | Immunoregulatory active heptapeptide and preparation method and application thereof |
| CN111978374B (en) * | 2020-07-29 | 2022-03-04 | 中国科学院华南植物园 | Immunoregulation active hexapeptide and preparation method and application thereof |
| CN114195860A (en) * | 2021-12-16 | 2022-03-18 | 吉林国瑞医药有限公司 | Anti-angiogenesis and anti-tumor active peptide from codonopsis pilosula thunb, and preparation method and application thereof |
| CN114213516A (en) * | 2021-12-16 | 2022-03-22 | 吉林国瑞医药有限公司 | Codonopsis lanceolata-derived lactagogue and breast tumor-resistant active peptide, and preparation method and application thereof |
| CN114195860B (en) * | 2021-12-16 | 2023-07-25 | 吉林国瑞医药有限公司 | Anti-angiogenesis and anti-tumor active peptide derived from codonopsis pilosula as well as preparation method and application thereof |
| CN114213516B (en) * | 2021-12-16 | 2023-07-25 | 吉林国瑞医药有限公司 | Active peptide derived from radix codonopsis pilosulae for promoting lactation and resisting breast tumor, and preparation method and application thereof |
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| CN110655556B (en) | 2021-05-18 |
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