CN110653863B - Hard tissue cutting device for schizothorax primary culture cell culture - Google Patents
Hard tissue cutting device for schizothorax primary culture cell culture Download PDFInfo
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- CN110653863B CN110653863B CN201910872732.2A CN201910872732A CN110653863B CN 110653863 B CN110653863 B CN 110653863B CN 201910872732 A CN201910872732 A CN 201910872732A CN 110653863 B CN110653863 B CN 110653863B
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- 238000005520 cutting process Methods 0.000 title claims abstract description 199
- 241001275867 Schizothorax Species 0.000 title claims abstract description 41
- 238000004113 cell culture Methods 0.000 title claims abstract description 20
- 241000016023 Schizothorax biddulphi Species 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 239000007769 metal material Substances 0.000 claims description 3
- 241001275865 Schizothorax prenanti Species 0.000 abstract description 4
- 239000002184 metal Substances 0.000 abstract description 3
- 241000251468 Actinopterygii Species 0.000 description 12
- 241001544485 Cordulegastridae Species 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 4
- 238000013461 design Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000007306 turnover Effects 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 206010015856 Extrasystoles Diseases 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 210000000038 chest Anatomy 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000010297 mechanical methods and process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B26—HAND CUTTING TOOLS; CUTTING; SEVERING
- B26D—CUTTING; DETAILS COMMON TO MACHINES FOR PERFORATING, PUNCHING, CUTTING-OUT, STAMPING-OUT OR SEVERING
- B26D1/00—Cutting through work characterised by the nature or movement of the cutting member or particular materials not otherwise provided for; Apparatus or machines therefor; Cutting members therefor
- B26D1/01—Cutting through work characterised by the nature or movement of the cutting member or particular materials not otherwise provided for; Apparatus or machines therefor; Cutting members therefor involving a cutting member which does not travel with the work
- B26D1/547—Cutting through work characterised by the nature or movement of the cutting member or particular materials not otherwise provided for; Apparatus or machines therefor; Cutting members therefor involving a cutting member which does not travel with the work having a wire-like cutting member
- B26D1/553—Cutting through work characterised by the nature or movement of the cutting member or particular materials not otherwise provided for; Apparatus or machines therefor; Cutting members therefor involving a cutting member which does not travel with the work having a wire-like cutting member with a plurality of wire-like cutting members
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B26—HAND CUTTING TOOLS; CUTTING; SEVERING
- B26D—CUTTING; DETAILS COMMON TO MACHINES FOR PERFORATING, PUNCHING, CUTTING-OUT, STAMPING-OUT OR SEVERING
- B26D5/00—Arrangements for operating and controlling machines or devices for cutting, cutting-out, stamping-out, punching, perforating, or severing by means other than cutting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B26—HAND CUTTING TOOLS; CUTTING; SEVERING
- B26D—CUTTING; DETAILS COMMON TO MACHINES FOR PERFORATING, PUNCHING, CUTTING-OUT, STAMPING-OUT OR SEVERING
- B26D5/00—Arrangements for operating and controlling machines or devices for cutting, cutting-out, stamping-out, punching, perforating, or severing by means other than cutting
- B26D5/08—Means for actuating the cutting member to effect the cut
- B26D5/086—Electric, magnetic, piezoelectric, electro-magnetic means
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B26—HAND CUTTING TOOLS; CUTTING; SEVERING
- B26D—CUTTING; DETAILS COMMON TO MACHINES FOR PERFORATING, PUNCHING, CUTTING-OUT, STAMPING-OUT OR SEVERING
- B26D7/00—Details of apparatus for cutting, cutting-out, stamping-out, punching, perforating, or severing by means other than cutting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B26—HAND CUTTING TOOLS; CUTTING; SEVERING
- B26D—CUTTING; DETAILS COMMON TO MACHINES FOR PERFORATING, PUNCHING, CUTTING-OUT, STAMPING-OUT OR SEVERING
- B26D7/00—Details of apparatus for cutting, cutting-out, stamping-out, punching, perforating, or severing by means other than cutting
- B26D7/08—Means for treating work or cutting member to facilitate cutting
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- Life Sciences & Earth Sciences (AREA)
- Forests & Forestry (AREA)
- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Processing Of Stones Or Stones Resemblance Materials (AREA)
Abstract
A hard tissue cutting device for primary cell culture of schizothorax bivalve comprises a bottom plate, wherein a vertical first groove is formed in the top surface of the left side of the bottom plate, fourth grooves are formed in the two sides of the first groove respectively, a second electric telescopic rod, a first power device, a second power device, a third power device and a transverse cutting device are connected with a power supply and a controller respectively, the vertical and longitudinal cutting of the schizothorax bivalve hard tissue is performed by adopting a metal cutting line, the adhesion of the cutting line to the schizothorax bivalve hard tissue is effectively avoided by utilizing the different rotating directions of the cutting line, fine operation is performed by adopting automatic control, the deviation during manual cutting can be effectively avoided, the schizothorax bivalve hard tissue is always kept in the cutting cylinder by adopting the matching mode of the cutting cylinder, a convex plate and a magnet plate, and the schizothorax bivalve hard tissue is prevented from contacting with the outside, can effectively reduce pollution and provide guarantee for the primary cell culture of the hard tissues of the schizothorax prenanti.
Description
Technical Field
The invention belongs to the field of primary cell culture, and particularly relates to a hard tissue cutting device for schizothorax prenanti primary cell culture.
Background
Primary cell culture refers to a biotechnology in which various tissues of an animal body are taken out of the body, treated by various enzymes, chelating agents or mechanical methods, dispersed into single cells, and cultured in a suitable culture medium to enable the cells to survive, grow and reproduce; with the development and progress of science and technology, the application range of primary cell culture is more and more extensive, and the primary cell culture becomes an important biotechnology; the hard animal tissue needs to be cut before primary cell culture, the cut hard animal tissue is a cube with the volume of one cubic millimeter, manual cutting is mostly adopted for cutting the hard animal tissue in the prior art, the manual cutting easily causes the uneven volume of each cut small block, and the pollution is easily caused, so that the subsequent research is influenced.
Disclosure of Invention
The invention provides a hard tissue cutting device for schizothorax primary cell culture, which is used for overcoming the defects in the prior art.
The invention is realized by the following technical scheme:
a hard tissue cutting device for schizothorax biddulphi primary cell culture comprises a bottom plate, wherein a vertical first groove is formed in the top surface of the left side of the bottom plate, horizontal fourth grooves are formed in the two sides of the first groove respectively, horizontal second electric telescopic rods are fixedly installed in the fourth grooves, the movable ends of the two second electric telescopic rods are opposite, a convex plate which is in contact fit with the first groove and has an inverted T-shaped cross section is arranged in the first groove, the convex plate is made of a magnet material, a plurality of groups of criss-cross third grooves are formed in the top surface of the convex plate, the top surface of the convex plate is divided into a plurality of small blocks with the area of one square millimeter by the third grooves, two first electric telescopic rods which are symmetrical along the horizontal center line of the bottom plate are fixedly installed on the right side of the top surface of the bottom plate, transverse plates are respectively fixedly installed with transverse plates, a cutting cylinder with two ends opened and vertical cross sections of the two transverse plates and is square is, the lower part of the cutting cylinder is provided with two groups of strip-shaped first through grooves which are distributed up and down, each group comprises four first through grooves which are positioned around the cutting cylinder, the first through grooves are communicated with the first annular groove and the outside at the same time, first line rollers which are parallel to the first through grooves are respectively arranged in the first through grooves, two ends of a rotating shaft of each first line roller are respectively connected with the side wall of the corresponding first through groove through bearings, and the side surface of the cutting cylinder is provided with a first power device for driving the first line roller with the axial direction as the left-right direction to rotate and a second power device for driving the first line roller with the axial direction as the front-back direction to rotate; the diameter of the periphery of the first wire roller is larger than the width of the first through groove, the bottom surface of the first wire roller positioned at the lower part is positioned above the bottom surface of the cutting cylinder, and annular first wire guide grooves are formed in the periphery of the first wire roller at equal intervals; the inner wall of the periphery of the lower part in the cutting cylinder is respectively provided with strip-shaped second through grooves, the second through grooves are in one-to-one correspondence with the first through grooves positioned at the bottom of the cutting cylinder, second line rollers parallel to the second through grooves are respectively arranged in the second through grooves, two ends of a rotating shaft of each second line roller are respectively connected with the side walls of the corresponding second through grooves through bearings, the second line rollers are in one-to-one correspondence with the first line rollers positioned at the bottom of the cutting cylinder, and the periphery of each second line roller is provided with second wire grooves in one-to-one correspondence with the first wire grooves; the four first wire rollers and the two second wire rollers which are parallel to each other are positioned at the first wire guide groove and the second wire guide groove of the same vertical surface and are connected through cutting wires, each cutting wire bypasses the lower side of the first wire roller corresponding to the lower side, the upper side of the first wire roller corresponding to the upper side and the lower side of the second wire roller corresponding to the upper side, the cutting wires are positioned in the first wire guide groove and the second wire guide groove corresponding to each other, and the distance between the two parallel cutting wires is one millimeter; a vertical second annular groove is formed in the inner wall of the upper portion of the cutting cylinder, a sleeve with a square cross section and an opening at the lower end is arranged in the second annular groove, the transverse length of the interior of the sleeve is the same as that of the interior of the cutting cylinder, a plurality of horizontal first through holes corresponding to the second annular groove are formed in the upper portion of the left side of the cutting cylinder, second through holes corresponding to the first through holes in a one-to-one mode are formed in the inner wall of the left side of the sleeve, and the second through holes are communicated with the interior of the sleeve and; the cutting cylinder is made of a metal material which can be adsorbed by a magnet, a T-shaped magnet plate which is attracted with the cutting cylinder is arranged above the cutting cylinder, horizontal fifth grooves are respectively formed in two sides of the magnet plate and the convex plate, the movable end of a second electric telescopic rod can be inserted into the corresponding fifth grooves, the front side and the rear side of the cutting cylinder are respectively connected with corresponding transverse plate bearings through a third connecting rod, a third driving device which drives the cutting cylinder to rotate around the third connecting rod is further arranged on the transverse plate, a transverse cutting device which can horizontally move and corresponds to the sleeve is further arranged between the two transverse plates, the transverse cutting device is positioned on the right side of the cutting cylinder, a controller is fixedly mounted on the front side of the transverse plate positioned on the front side, and the second electric telescopic rod, the first power device, the second power device, the third power device and the transverse cutting device are respectively connected with a power supply and the controller.
The third driving device comprises a first bevel gear, a first motor and a second bevel gear, wherein the third connecting rod at the rear side of the cutting cylinder is connected with the inner side of the corresponding transverse plate through a bearing, the front end of the third connecting rod at the front side penetrates through the corresponding transverse plate and is connected with the transverse plate through a bearing, the first bevel gear is fixedly installed at the front end of the third connecting rod at the front side, the first motor is fixedly installed in front of the transverse plate at the front side, the output shaft of the first motor faces right, the second bevel gear is fixedly installed at the output end of the first motor, the second bevel gear is meshed with the first bevel gear, and the first motor is respectively connected with a power supply and a controller.
The hard tissue cutting device for culturing the schizothorax primary cell comprises a first power device, a second power device and a third bevel gear, wherein the first power device comprises a third bevel gear, a second motor and a fourth bevel gear, the left end of a first wire roller rotating shaft positioned in the middle of the rear surface of a cutting cylinder penetrates through the side surface of a corresponding first through groove and is in bearing connection with the first through groove, the left end of the first wire roller rotating shaft positioned in the middle of the left side surface of the cutting cylinder is fixedly provided with the third bevel gear, the left side of the cutting cylinder is fixedly provided with the second motor, an output shaft of the second motor faces backwards, the output shaft of the second motor is fixedly provided with the fourth bevel gear, the fourth bevel gear is meshed with the third bevel gear, and the second motor is respectively connected with a power.
The hard tissue cutting device for schizothorax prenanti primary cell culture comprises a first gear, a second motor and a second gear, wherein the front end of a first wire roller rotating shaft positioned in the middle of the left side of a cutting cylinder penetrates through the corresponding side surface of a first through groove and is connected with the side surface of the first through groove through a bearing, the front end of the first wire roller rotating shaft positioned in the middle of the left side surface of the cutting cylinder is fixedly provided with the first gear, the left side of the cutting cylinder is fixedly provided with a third motor, an output shaft of the second motor faces backwards, an output shaft of the second motor is fixedly provided with the second gear, the second gear is meshed with the first gear, and the third motor is respectively connected with a power supply and a controller.
The transverse cutting device comprises first guide rails, first electric slide blocks, guide rods, second guide rails, second electric slide blocks, vertical plates, guide grooves and blades, wherein the two first guide rails are fixedly installed on the top surface of a bottom plate and are distributed in a front-back parallel manner, the first guide rails are positioned on the inner sides of the two first electric telescopic rods, the upper ends of the first guide rails are respectively provided with the first electric slide blocks which are movably matched with the first guide rails, the top surfaces of the first electric slide blocks are respectively fixedly installed with the guide rods, one sides, opposite to the two transverse plates, of the two transverse plates are respectively fixedly installed with the horizontal second guide rails, the inner sides of the second guide rails are respectively provided with the second electric slide blocks which are movably matched with the second electric slide blocks, the vertical guide grooves are respectively formed in the front side and the back side of the bottom surface of the vertical plates, the upper ends of the guide rods are respectively inserted into the corresponding guide grooves and are in contact fit with the guide grooves, the left side fixed mounting array of riser is the blade of vertical equidistant range, and the interval between two adjacent blades is a millimeter, and first electronic slider, second electronic slider are connected with power, controller respectively.
The invention has the advantages that: before the cutting machine is put into use, a user places prepared schizothorax upon the top surface of a convex plate, when the cutting machine is used, as shown in figure 1, the movable end of a second electric telescopic rod is inserted into a fifth groove corresponding to the convex plate, a controller controls a first electric telescopic rod to move downwards, and simultaneously a first power device and a second electric device respectively drive a corresponding first wire roller to rotate, so that each group of wire rollers with the axial directions of left and right directions and the wire rollers with the axial directions of front and back directions drive corresponding cutting wires to rotate, when the bottom surface of a cutting cylinder contacts with the top surface of the schizothorax upon, the cutting wires gradually contact with the top surface of the schizothorax upon moving downwards along with the cutting cylinder, because each cutting wire is respectively wound on the periphery of the bottom surface of the first wire roller at the bottom of the cutting cylinder, the periphery of the top surface of the first wire roller at the middle of the cutting cylinder and the periphery of the bottom surface of the corresponding second wire roller, the cutting line on the bottom surface of the first line roller at the bottom of the cutting cylinder and the cutting line on the bottom surface of the second line roller in the same group have different rotating directions, and the cutting line cannot be adhered to the schizothorax fish hard tissue in the cutting process, so that the schizothorax fish hard tissue can be cut in the up-down direction and the front-back direction, the original schizothorax fish hard tissue is cut into a cylinder with the vertical length and width equal to one millimeter, and the movable end of the second electric telescopic rod is inserted into the fifth groove corresponding to the convex plate, so that the convex plate is always positioned in the first groove in the front-back direction and the up-down direction during the cutting process; after the cutting is finished, the second electric telescopic rod contracts and is separated from the convex plate, the convex plate is adsorbed on the bottom surface of the cutting cylinder, and a third groove corresponding to the cutting line is formed in the top surface of the convex plate, so that the complete vertical cutting of the hard schizothorax tissue by the cutting line can be ensured, at the moment, the first electric telescopic rod drives the whole cutting cylinder to move upwards, the controller controls the third power device to start working, the third power device enables the cutting cylinder to turn over by one hundred eighty degrees towards the right side around a corresponding third connecting rod, so that the magnet plate turns over to the bottom of the cutting cylinder, the hard schizothorax tissue cut in the previous step is turned over into the sleeve from the lower part of the cutting cylinder, the first electric telescopic rod drives the cutting cylinder to move downwards until the magnet plate is contacted with the bottom surface of the first groove, the second electric telescopic rod works, the movable end of the second electric telescopic rod respectively moves towards the fifth groove corresponding to the magnet plate, and the transverse cutting device, the transverse cutting device transversely cuts the schizothorax hard tissue in the sleeve, and the schizothorax hard tissue column body with the length and the width of one millimeter is cut into schizothorax hard tissue small blocks with the length, the width and the height of one millimeter, so that the schizothorax hard tissue is cut into small blocks with the length of one cubic millimeter, and the operation is finished; the splitting fish hard tissue culture device is reasonable in design, the metal cutting line is used for vertically and longitudinally cutting the splitting fish hard tissue, the cutting line is effectively prevented from being adhered to the splitting fish hard tissue due to the fact that the cutting line rotates in different directions, meanwhile, fine operation is conducted through automatic control, deviation caused by manual cutting can be effectively avoided, the splitting fish hard tissue is always kept in the cutting cylinder due to the fact that the cutting cylinder is matched with the convex plate and the magnet plate, the splitting fish hard tissue is prevented from being in contact with the outside, pollution can be effectively reduced, and primary cell culture of the subsequent splitting fish hard tissue is guaranteed.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic structural view of the present invention; FIG. 2 is a schematic structural view of a cutting drum; FIG. 3 is an enlarged view of the view from the direction A of FIG. 1; FIG. 4 is an enlarged view of a portion of FIG. 1I; FIG. 5 is an enlarged view of the view of FIG. 2 taken along line B; FIG. 6 is an enlarged view of a portion of FIG. 2 II; FIG. 7 is an enlarged view of a portion of FIG. 5 III; FIG. 8 is a schematic structural view of a first power plant; fig. 9 is a schematic structural diagram of the second power unit.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
A hard tissue cutting device for schizothorax prenanti primary cell culture comprises a bottom plate 1, wherein a vertical first groove is formed in the top surface of the left side of the bottom plate 1, horizontal fourth grooves are formed in the two sides of the first groove respectively, horizontal second electric telescopic rods 2 are fixedly installed in the fourth grooves, the movable ends of the two second electric telescopic rods 2 are opposite, a convex plate 3 which is in contact fit with the first groove and has an inverted T-shaped cross section is arranged in the first groove, the convex plate 3 is made of a magnet material, a plurality of groups of criss-cross third grooves are formed in the top surface of the convex plate 3, the top surface of the convex plate 3 is divided into a plurality of small blocks with one square millimeter area by the third grooves, two first electric telescopic rods 4 which are symmetrical along the horizontal center line of the bottom plate 1 are fixedly installed on the right side of the top surface of the bottom plate 1, transverse plates 5 are fixedly installed on the top surface of the first electric telescopic rods 4 respectively, a cutting cylinder 6 with two ends open and vertical, the bottom surface of the cutting cylinder 6 is provided with a vertical first annular groove 7, the lower part of the cutting cylinder 6 is provided with two groups of strip-shaped first through grooves 8 which are distributed up and down, each group comprises four first through grooves 8 positioned around the cutting cylinder 6, the first through grooves 8 are communicated with the first annular groove 7 and the outside at the same time, the first through grooves 8 are internally provided with first line rollers 9 which are parallel to the first through grooves 8 respectively, two ends of a rotating shaft of each first line roller 9 are connected with the side walls of the corresponding first through groove 8 respectively through bearings, and the side surface of the cutting cylinder 6 is provided with a first power device for driving the first line rollers 9 which are axially in the left-right direction to rotate and a second power device for driving the first line rollers 9 which are axially in the front-back direction to rotate; the diameter of the outer periphery of the first wire roller 9 is larger than the width of the first through groove 8, the bottom surface of the first wire roller 9 positioned at the lower part is positioned above the bottom surface of the cutting cylinder 6, and annular first wire guide grooves 13 are formed in the outer periphery of the first wire roller 9 at equal intervals; the inner wall of the periphery of the lower part in the cutting cylinder 6 is respectively provided with strip-shaped second through grooves 10, the second through grooves 10 are in one-to-one correspondence with first through grooves 8 positioned at the bottom of the cutting cylinder 6, second line rollers 11 parallel to the second through grooves 10 are respectively arranged in the second through grooves 10, two ends of a rotating shaft of each second line roller 11 are respectively connected with the side wall of the corresponding second through groove 10 through a bearing, the second line rollers 11 are in one-to-one correspondence with first line rollers 9 positioned at the bottom of the cutting cylinder 6, and the periphery of each second line roller 11 is provided with second wire grooves 14 corresponding to the first wire grooves 13; the four parallel first wire rollers 9 and two second wire rollers 11 are positioned at the first wire guide groove 13 and the second wire guide groove 14 of the same vertical surface and are connected through cutting wires 12, each cutting wire 12 bypasses the lower side of the first wire roller 9 corresponding to the lower side, the upper side of the first wire roller 9 corresponding to the upper side and the lower side of the corresponding second wire roller 11, the cutting wires 12 are positioned in the corresponding first wire guide groove 13 and the second wire guide groove 14, and the distance between the two parallel cutting wires 12 is one millimeter; a vertical second annular groove 15 is formed in the inner wall of the upper portion of the cutting barrel 6, a sleeve 16 with a square cross section and an opening at the lower end is arranged in the second annular groove 15, the transverse length of the interior of the sleeve 16 is the same as that of the interior of the cutting barrel 6, a plurality of horizontal first through holes 17 corresponding to the second annular groove 15 are formed in the upper portion of the left side of the cutting barrel 6, second through holes 18 corresponding to the first through holes 17 in a one-to-one mode are formed in the inner wall of the left side of the sleeve 16, and the second through holes 18 are communicated with the interior of the sleeve 16 and; the cutting cylinder 6 is made of metal material which can be adsorbed by magnet, a T-shaped magnet plate 19 which is attracted with the cutting cylinder 6 is arranged above the cutting cylinder 6, horizontal fifth grooves 20 are respectively arranged on two sides of the magnet plate 19 and the convex plate 3, the movable end of the second electric telescopic rod 2 can be inserted into the corresponding fifth grooves 20, the front side and the rear side of the cutting cylinder 6 are respectively connected with the corresponding transverse plate 5 through a third connecting rod 39 in a bearing mode, a third driving device which drives the cutting cylinder 6 to rotate around the third connecting rod 39 is further arranged on the transverse plate 5, a horizontal cutting device which can horizontally move and corresponds to the sleeve 16 is further arranged between the two transverse plates 5, the horizontal cutting device is positioned on the right side of the cutting cylinder 6, a controller 24 is fixedly arranged on one side of the front face of the transverse plate 5 positioned on the front side, and the second electric telescopic rod 2, the first electric telescopic rod 4, the first power device, the second power device and the third power device are, The transverse cutting device is connected with a power supply and a controller 24 respectively. Before the present invention is put into use, a user places prepared schizothorax upon the top surface of the convex plate 3, and when the present invention is used, as shown in fig. 1, the movable end of the second electric telescopic rod 2 is inserted into the corresponding fifth groove 20 of the convex plate 3, the controller 24 controls the first electric telescopic rod 4 to move downwards, and simultaneously the first power device and the second electric power device respectively drive the corresponding first wire roller 9 to rotate, so that each group of wire rollers with axial directions of left and right directions and the wire rollers with axial directions of front and back directions drive the corresponding cutting wires 12 to rotate, when the bottom surface of the cutting cylinder 6 contacts with the top surface of the schizothorax upon, the cutting wires 12 gradually contact with the top surface of the schizothorax upon the bottom surface of the cutting cylinder 6, because each cutting wire 12 is respectively wound around the wire rollers with left and right directions and the wire rollers with axial directions of front and back directions and is positioned on the outer periphery of the bottom surface of the first wire roller 9 of the cutting cylinder 6, The top surface periphery of the first wire roller 9 positioned in the middle of the cutting cylinder 6 and the bottom surface periphery of the corresponding second wire roller 11 are different in rotating direction of the cutting line 12 positioned on the bottom surface of the first wire roller 9 at the bottom of the cutting cylinder 6 and the cutting line 12 positioned on the bottom surface of the second wire roller 11 in the same group, and the cutting line 12 cannot be adhered to the schizothorax biddy hard tissue in the cutting process, so that the schizothorax biddy hard tissue can be cut in the vertical direction and the front-back direction, the original schizothorax biddy hard tissue is cut into a column body with the vertical length and width equal to one millimeter, and the movable end of the second electric telescopic rod 2 is inserted into the fifth groove 20 corresponding to the convex plate 3, so that the convex plate 3 is always positioned in the first groove in the cutting process in the front-back direction and the up-down direction; after the cutting is finished, the second electric telescopic rod 2 is contracted to be separated from the convex plate 3, the convex plate 3 is adsorbed on the bottom surface of the cutting cylinder 6, and a third groove corresponding to the cutting line 12 is formed in the top surface of the convex plate 3, so that the cutting line 12 can be ensured to completely cut the hard schizothorax tissue vertically, at the moment, the first electric telescopic rod 4 drives the whole cutting cylinder 6 to move upwards, the controller 24 controls the third power device to start working, the third power device enables the cutting cylinder 6 to turn over by one hundred eighty degrees around the corresponding third connecting rod 20 to the right side, so that the magnet plate 19 is turned over to the bottom of the cutting cylinder 6, the hard thorax tissue cut in the previous step is turned over into the sleeve 16 from the lower part of the cutting cylinder 6, the first electric telescopic rod 4 drives the cutting cylinder 6 to move downwards until the magnet plate 19 is contacted with the bottom surface of the first groove, the second electric telescopic rod 2 works, the movable end of the second electric telescopic rod 2 respectively moves towards the fifth groove 20 corresponding to the, meanwhile, the transverse cutting device on the right side of the bottom plate 1 moves to the left side, transversely cuts the schizothorax hard tissue in the sleeve 16, and cuts the schizothorax hard tissue column body with the length and the width of one millimeter into schizothorax hard tissue small blocks with the length, the width and the height of 1 millimeter, so that the schizothorax hard tissue is cut into small blocks with the length, the width and the height of one cubic millimeter, and the operation is finished; the splitting fish hard tissue culture device is reasonable in design, the metal cutting line 12 is used for vertically and longitudinally cutting the splitting fish hard tissue, the cutting line 12 is effectively prevented from being adhered to the splitting fish hard tissue due to the fact that the cutting line 12 rotates in different directions, meanwhile, fine operation is conducted through automatic control, deviation caused by manual cutting can be effectively avoided, the splitting fish hard tissue is always kept in the cutting cylinder 6 due to the fact that the cutting cylinder 6 is matched with the convex plate 3 and the magnet plate 19, the splitting fish hard tissue is prevented from being in contact with the outside, pollution can be effectively reduced, and primary cell culture of the following splitting fish hard tissue is guaranteed.
Specifically, as shown in fig. 1, the third driving device according to this embodiment includes a first bevel gear 21, a first motor 22, and a second bevel gear 23, wherein a third connecting rod 39 located at the rear side of the cutting cylinder 6 is bearing-connected to the inner side of the corresponding transverse plate 5, the front end of the front third connecting rod 39 penetrates through and is bearing-connected to the corresponding transverse plate 5, the front end of the front third connecting rod 39 is fixedly mounted with the first bevel gear 21, the first motor 22 is fixedly mounted in front of the front transverse plate 5, the output shaft of the first motor 22 faces to the right, the output end of the first motor 22 is fixedly mounted with the second bevel gear 23, the second bevel gear 23 is engaged with the first bevel gear 21, and the first motor 22 is respectively connected to the power supply and the controller 24. In the using process, the controller 24 enables the first motor 22 to start to work, and as the second bevel gear 23 is meshed and matched with the first bevel gear 21, the rotation of the output shaft of the first motor 22 drives the corresponding third connecting rod 39 to rotate, and the rotation of the third connecting rod 39 drives the cutting cylinder 6 to rotate by one hundred eighty degrees, so that the hard tissue of the schizothorax fish in the cutting cylinder 6 is overturned by one hundred eighty degrees.
Specifically, as shown in fig. 8, the first power device according to this embodiment includes a third bevel gear 25, a second motor 27, and a fourth bevel gear 26, the left end of the rotating shaft of the first wire roller 9 located at the middle portion of the rear surface of the cutting cylinder 6 penetrates through the side surface of the corresponding first through groove 8 and is connected with the side surface of the corresponding first through groove by a bearing, the third bevel gear 25 is fixedly installed at the left end of the rotating shaft of the first wire roller 9 located at the middle portion of the left side surface of the cutting cylinder 6, the second motor 27 is fixedly installed at the left side of the cutting cylinder 6, the output shaft of the second motor 27 faces backwards, the fourth bevel gear 26 is fixedly installed at the output shaft of the second motor 27, the fourth bevel gear 26 is engaged with the third bevel gear 25, and the second motor 27. In the using process, the controller 24 enables the second motor 27 to work, because the fourth bevel gear 26 is meshed with the third bevel gear 25, the rotation of the output shaft of the second motor 27 drives the first wire roller 9 positioned in the middle of the back of the cutting cylinder 6 to rotate, because all horizontal first wire rollers 9 and second wire rollers 11 on the cutting cylinder 6 are a group of wire rollers with the axial direction being the left and right direction, and each group of wire rollers with the axial direction being the left and right direction are connected by a plurality of cutting lines 12 distributed at equal intervals, all the wire rollers with the axial direction being the left and right direction on the cutting cylinder 6 rotate, and accordingly, the hard schizothorax fish tissues are cut in the front and back direction through the cutting lines 12.
Further, as shown in fig. 9, the second power device described in this embodiment includes a first gear 28, a third motor 30, and a second gear 29, wherein the front end of the rotating shaft of the first thread roller 9 located in the middle of the left side of the cutting drum 6 penetrates through the corresponding side of the first through groove 8 and is connected with the side through a bearing, the front end of the rotating shaft of the first thread roller 9 located in the middle of the left side of the cutting drum 6 is fixedly installed with the first gear 28, the left side of the cutting drum 6 is fixedly installed with the third motor 30, the output shaft of the third motor 30 faces backwards, the output shaft of the third motor 30 is fixedly installed with the second gear 29, the second gear 29 is engaged with the first gear 28, and the third motor 30 is respectively connected with the power supply and the controller 24. In the use, controller 24 makes third motor 30 work, because second gear 29 and the meshing cooperation of first gear 28, the rotation of third motor 30 output shaft drives the first line roller 9 that is located cutting barrel 6 left side middle part and rotates, because first line roller 9 and the second line roller 11 that all axial are fore-and-aft direction on the cutting barrel 6 are a set of axial and are the line roller of fore-and-aft direction, and every group axial is the line roller of cut 12 that the several equidistant distributions were connected for the line roller of cut of fore-and-aft direction, thereby make all axial on the cutting barrel 6 all rotate for the line roller of fore-and-aft direction, thereby realize the cutting of upper and lower direction through line of cut 12 to schizothorax bigeminy hard tissue.
Furthermore, as shown in fig. 1, the transverse cutting device according to this embodiment includes a first guide rail 31, a first electric slider 32, a guide rod 33, a second guide rail 38, a second electric slider 34, a vertical plate 35, a guide groove 36, and a blade 37, wherein two first guide rails 31 are fixedly installed on the top surface of the base plate 1, the two first guide rails 31 are distributed in parallel front and back, the first guide rail 31 is located inside the two first electric telescopic rods 4, the upper end of the first guide rail 31 is respectively provided with the first electric slider 32 movably engaged therewith, the top surface of the first electric slider 32 is respectively fixedly installed with the guide rod 33, the opposite sides of the two transverse plates 5 are respectively fixedly installed with a horizontal second guide rail 38, the inner sides of the second guide rails 38 are respectively provided with the second electric slider 34 movably engaged therewith, the vertical plate 35 is fixedly installed between the second electric sliders 34, the front and back sides of the bottom surface of the vertical plate 35 are respectively provided with a vertical guide groove, the upper ends of the guide rods 33 are respectively inserted into the corresponding guide grooves 36 and are in contact fit with the guide grooves, a plurality of groups of blades 37 which are vertically arranged at equal intervals are fixedly installed on the left side of the vertical plate 35, the interval between every two adjacent blades 37 is one millimeter, and the first electric slide block 32 and the second electric slide block 34 are respectively connected with the power supply and the controller 24. When the hard schizothorax biddulphi tissue needs to be vertically cut, the cutting cylinder 6 is turned over by one hundred eighty degrees, so that the first through hole 17 and the second through hole 18 correspond to the corresponding blade 37, the controller 24 controls the first electric slide block 32 to move leftwards along the corresponding first guide rail 31 and the second electric slide block 34 through controlling the first electric slide block 32 and the corresponding second guide rail 38, so that the blades 37 are sequentially inserted into the corresponding first through hole 17 and the second through hole 18 and transversely cut the hard schizothorax biddi tissue, and the height of the hard schizothorax biddle tissue between the two adjacent blades 37 is one millimeter due to the fact that the distance between the two adjacent blades 37 is one millimeter, and therefore the transverse cutting of the hard schizothorax biddi tissue is completed.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (4)
1. A hard tissue cutting device for schizothorax biddulphi primary cell culture is characterized in that: comprises a bottom plate (1), wherein a vertical first groove is formed in the top surface of the left side of the bottom plate (1), horizontal fourth grooves are respectively formed in two sides of the first groove, a horizontal second electric telescopic rod (2) is fixedly installed in the fourth groove, the movable ends of the two second electric telescopic rods (2) are opposite, a convex plate (3) which is in contact fit with the first groove and has an inverted T-shaped cross section is arranged in the first groove, the convex plate (3) is made of a magnet material, a plurality of groups of criss-cross third grooves are formed in the top surface of the convex plate (3), the top surface of the convex plate (3) is divided into a plurality of small blocks with an area of one square millimeter by the third grooves, two first electric telescopic rods (4) which are symmetrical along the horizontal central line of the bottom plate (1) are fixedly installed on the right side of the top surface of the bottom plate (1), transverse plates (5) are respectively and fixedly installed on the top surface of the first electric telescopic rods (4), a cutting cylinder (6, the bottom surface of the cutting cylinder (6) is provided with a vertical first annular groove (7), the lower part of the cutting cylinder (6) is provided with two groups of strip-shaped first through grooves (8) which are distributed up and down, each group comprises four first through grooves (8) which are positioned around the cutting cylinder (6), the first through grooves (8) are simultaneously communicated with the first annular groove (7) and the outside, the first through grooves (8) are internally provided with first wire rollers (9) which are parallel to the first through grooves, two ends of a rotating shaft of each first wire roller (9) are respectively in bearing connection with the side walls of the corresponding first through groove (8), and the side surface of the cutting cylinder (6) is provided with a first power device for driving the first wire rollers (9) to rotate in the left-right direction in the axial direction and a second power device for driving the first wire rollers (9) to rotate in the front-back direction in the axial direction; the diameter of the outer periphery of the first wire roller (9) is larger than the width of the first through groove (8), the bottom surface of the first wire roller (9) positioned at the lower part is positioned above the bottom surface of the cutting cylinder (6), and annular first wire grooves (13) are formed in the outer periphery of the first wire roller (9) at equal intervals; the inner wall of the periphery of the lower part in the cutting cylinder (6) is respectively provided with strip-shaped second through grooves (10), the second through grooves (10) are in one-to-one correspondence with first through grooves (8) positioned at the bottom of the cutting cylinder (6), second wire rollers (11) parallel to the second through grooves are respectively arranged in the second through grooves (10), two ends of a rotating shaft of each second wire roller (11) are respectively connected with the side wall of the corresponding second through groove (10) through bearings, the second wire rollers (11) are in one-to-one correspondence with first wire rollers (9) positioned at the bottom of the cutting cylinder (6), and the periphery of each second wire roller (11) is provided with second wire grooves (14) corresponding to first wire grooves (13); the four first wire rollers (9) and the two second wire rollers (11) which are parallel to each other are positioned in a first wire guide groove (13) and a second wire guide groove (14) of the same vertical surface and are connected through cutting wires (12), each cutting wire (12) bypasses the lower side of the first wire roller (9) corresponding to the lower side, the upper side of the first wire roller (9) corresponding to the upper side and the lower side of the corresponding second wire roller (11), the cutting wires (12) are positioned in the corresponding first wire guide groove (13) and the corresponding second wire guide groove (14), and the distance between the two parallel cutting wires (12) is one millimeter; a vertical second annular groove (15) is formed in the inner wall of the upper portion of the cutting barrel (6), a sleeve (16) with a square cross section and an opening at the lower end is arranged in the second annular groove (15), the transverse length of the interior of the sleeve (16) is the same as that of the interior of the cutting barrel (6), a plurality of horizontal first through holes (17) corresponding to the second annular groove (15) are formed in the upper portion of the left side of the cutting barrel (6), second through holes (18) corresponding to the first through holes (17) in a one-to-one mode are formed in the inner wall of the left side of the sleeve (16), and the second through holes (18) are communicated with the interior of the sleeve (16) and the interior; the cutting barrel (6) is made of metal materials capable of being adsorbed by magnets, a T-shaped magnet plate (19) capable of being attracted with the cutting barrel is arranged above the cutting barrel (6), horizontal fifth grooves (20) are formed in the two sides of the magnet plate (19) and the convex plate (3) respectively, the movable end of the second electric telescopic rod (2) can be inserted into the corresponding fifth grooves (20), the front side and the rear side of the cutting barrel (6) are connected with corresponding transverse plate (5) through third connecting rods (39) respectively in a bearing mode, a third driving device capable of driving the cutting barrel (6) to rotate around the third connecting rods (39) is further arranged on the transverse plate (5), a transverse cutting device capable of horizontally moving and corresponding to the sleeve (16) is further arranged between the two transverse plates (5), the transverse cutting device is located on the right side of the cutting barrel (6), and comprises a first guide rail (31), a first electric sliding block (32) and a second electric sliding block, The guide device comprises guide rods (33), second guide rails (38), second electric sliders (34), vertical plates (35), guide grooves (36) and blades (37), wherein two first guide rails (31) are fixedly installed on the top surface of a bottom plate (1), the two first guide rails (31) are distributed in parallel front and back, the first guide rails (31) are positioned on the inner sides of the two first electric telescopic rods (4), first electric sliders (32) which are movably matched with the first guide rails are respectively arranged at the upper ends of the first guide rails (31), the guide rods (33) are respectively fixedly installed on the top surfaces of the first electric sliders (32), horizontal second guide rails (38) are respectively fixedly installed on one sides, opposite to the two transverse plates (5), of the second guide rails (38) are respectively provided with second electric sliders (34) which are movably matched with the second guide rails, the vertical plates (35) are fixedly installed between the second electric sliders (34), the guide grooves (36) are respectively formed in the front and back two sides of the bottom surface, the upper end of guide bar (33) inserts corresponding guide way (36) respectively and cooperates with it contact, the left side fixed mounting array of riser (35) is blade (37) of vertical equidistant range, interval between two adjacent blade (37) is a millimeter, first electric slider (32), second electric slider (34) respectively with the power, controller (24) are connected, be located preceding one side fixed mounting controller (24) of diaphragm (5) of front side, second electric telescopic handle (2), first electric telescopic handle (4), first power device, second power device, third power device, horizontal cutting device respectively with the power, controller (24) are connected.
2. The hard tissue cutting device for schizothorax bivalve primary cell culture as claimed in claim 1, wherein: the third driving device comprises a first bevel gear (21), a first motor (22) and a second bevel gear (23), a third connecting rod (39) positioned at the rear side of the cutting cylinder (6) is in bearing connection with the inner side of the corresponding transverse plate (5), the front end of the third connecting rod (39) at the front side penetrates through the corresponding transverse plate (5) and is in bearing connection with the transverse plate, the first bevel gear (21) is fixedly installed at the front end of the third connecting rod (39) at the front side, the first motor (22) is fixedly installed in front of the transverse plate (5) at the front side, the output shaft of the first motor (22) faces right, the second bevel gear (23) is fixedly installed at the output end of the first motor (22), the second bevel gear (23) is in meshing fit with the first bevel gear (21), and the first motor (22) is respectively connected with a power supply and a controller (24).
3. The hard tissue cutting device for schizothorax bivalve primary cell culture as claimed in claim 1, wherein: the first power device comprises a third bevel gear (25), a second motor (27) and a fourth bevel gear (26), the left end of a rotating shaft of a first wire roller (9) positioned in the middle of the rear surface of the cutting cylinder (6) penetrates through the side surface of a corresponding first through groove (8) and is in bearing connection with the first through groove, the third bevel gear (25) is fixedly installed at the left end of the rotating shaft of the first wire roller (9) positioned in the middle of the left side surface of the cutting cylinder (6), the second motor (27) is fixedly installed at the left side of the cutting cylinder (6), an output shaft of the second motor (27) faces backwards, the fourth bevel gear (26) is fixedly installed at an output shaft of the second motor (27), the fourth bevel gear (26) is in meshing fit with the third bevel gear (25), and the second motor (27) is respectively connected with a power supply and a controller (24).
4. The hard tissue cutting device for schizothorax bivalve primary cell culture as claimed in claim 1, wherein: the second power device comprises a first gear (28), a third motor (30) and a second gear (29), wherein the front end of a rotating shaft of a first wire roller (9) positioned in the middle of the left side of the cutting cylinder (6) penetrates through the side face of a corresponding first through groove (8) and is connected with the side face of the corresponding first through groove through a bearing, the front end of the rotating shaft of the first wire roller (9) positioned in the middle of the left side face of the cutting cylinder (6) is fixedly provided with the first gear (28), the left side of the cutting cylinder (6) is fixedly provided with the third motor (30), an output shaft of the third motor (30) faces backwards, the output shaft of the third motor (30) is fixedly provided with the second gear (29), the second gear (29) is meshed with the first gear (28) in a matched mode, and the third motor (30) is respectively connected with a power supply and a controller.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910872732.2A CN110653863B (en) | 2019-09-16 | 2019-09-16 | Hard tissue cutting device for schizothorax primary culture cell culture |
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| CN201910872732.2A CN110653863B (en) | 2019-09-16 | 2019-09-16 | Hard tissue cutting device for schizothorax primary culture cell culture |
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| CN110653863A CN110653863A (en) | 2020-01-07 |
| CN110653863B true CN110653863B (en) | 2021-04-23 |
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| CN201895340U (en) * | 2010-01-14 | 2011-07-13 | 许庆原 | Dicing machine |
| CN103231399A (en) * | 2013-05-10 | 2013-08-07 | 上海电机学院 | Bean curd dicer |
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| CN208592900U (en) * | 2018-08-16 | 2019-03-12 | 湖北十品佳农业发展股份有限公司 | A kind of dicer |
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| US4646602A (en) * | 1985-10-07 | 1987-03-03 | Bleick Robert O | Cheese cutter |
| CN201217225Y (en) * | 2008-07-11 | 2009-04-08 | 段超 | Bean curd cutting machine |
| CN201895340U (en) * | 2010-01-14 | 2011-07-13 | 许庆原 | Dicing machine |
| CN103231399A (en) * | 2013-05-10 | 2013-08-07 | 上海电机学院 | Bean curd dicer |
| CN208446548U (en) * | 2017-12-20 | 2019-02-01 | 浙江金苞谷食品有限公司 | A kind of cutter device producing steamed sponge cake |
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