CN110652855A - Compound formaldehyde scavenger containing thuja essential oil and preparation method thereof - Google Patents
Compound formaldehyde scavenger containing thuja essential oil and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a compound formaldehyde scavenger containing thuja essential oil, which comprises the following raw materials in parts by weight: 10-20 parts of soybean protein, 20-40 parts of plant leaf protein, 1-10 parts of compound essential oil, 0.2-1 part of sodium hypochlorite, 0.2-1 part of protease, 0.2-1 part of active carbon, 1-5 parts of nano antibacterial agent and 50-80 parts of purified water; the compound essential oil is arborvitae essential oil, mint essential oil and thyme essential oil according to the mass ratio of (2-4): 1: 1, mixing the mixture; the plant leaf protein is one or two of alfalfa leaf protein and pumpkin leaf protein. Also discloses a preparation method of the product. The product of the invention can actively absorb, absorb and capture formaldehyde, and then react with formaldehyde, well solve the problem of indoor air pollution, simultaneously promote the increase of immune protein, effectively regulate the balance of respiration and central nervous system, protect the mucous membrane of oral cavity and throat, and inhibit the growth of various tumor cells.
Description
Technical Field
The invention relates to the technical field of formaldehyde scavengers, in particular to a compound formaldehyde scavenger containing thuja essential oil and a preparation method thereof.
Background
Formaldehyde is one of the main indoor Volatile Organic Compounds (VOCs) and has a carcinogenic risk, so its removal is important for improving indoor air quality and human health, and has become one of the international concerns. Formaldehyde has been listed as the first carcinogen by the international agency for research on cancer (ARC) in 2004. Formaldehyde causes nasopharyngeal carcinoma, nasal cavity cancer and sinus cancer in humans and can cause leukemia. Along with the enhancement of the environmental awareness of people, the harm of formaldehyde released from the artificial board to human bodies draws more and more attention of people, the formaldehyde in the urea-formaldehyde resin adhesive has irritating smell which is unbearable, and has stimulating effects on nasal mucosa, retina and upper respiratory tract. It can react with protein to produce N-methylene compound to change the property of protein, and can cause chronic poisoning, pathological change and anaphylactic reaction to damage human health. For a long time, the harm of harmful substances such as formaldehyde and the like in decoration pollution is gradually known by people, the indoor formaldehyde is mostly from various artificial boards, the coating and the paint also contain certain formaldehyde, the formaldehyde is a substance with higher toxicity, and the formaldehyde is the second place on the priority control list of toxic chemicals in China. Formaldehyde has been identified by the world health organization as a carcinogenic and teratogenic substance, a recognized source of allergy, and also as one of the potentially strong mutagens.
Currently, in the formaldehyde scavenger products on the market, residual formaldehyde is usually eliminated by a physical adsorption or chemical adsorption treatment method, but the following technical defects exist: (1) physical adsorption is adopted, such as activated carbon, bamboo charcoal and the like, the adsorption capacity is limited, the use is inconvenient, and the secondary pollution is formed because the adsorption needs to be replaced frequently; (2) chemical adsorption is adopted, and although the formaldehyde treatment effect is good, the toxicity of the compound added into the scavenging agent is high, and the scavenging agent is not environment-friendly.
Disclosure of Invention
In view of the defects of the prior art, the invention aims to provide a compound formaldehyde scavenger containing thuja essential oil.
In order to achieve the purpose, the invention adopts the following technical scheme:
a compound formaldehyde scavenger containing thuja essential oil comprises the following raw materials in parts by weight: 10-20 parts of soybean protein, 20-40 parts of plant leaf protein, 1-10 parts of compound essential oil, 0.2-1 part of sodium hypochlorite, 0.2-1 part of protease, 0.2-1 part of active carbon, 1-5 parts of nano antibacterial agent and 50-80 parts of purified water;
preferably, the feed comprises the following raw materials in parts by weight: 15 parts of soybean protein, 30 parts of plant leaf protein, 5 parts of compound essential oil, 0.5 part of sodium hypochlorite, 0.5 part of protease, 0.5 part of activated carbon, 3 parts of nano antibacterial agent and 70 parts of purified water.
Preferably, the feed comprises the following raw materials in parts by weight: 10 parts of soybean protein, 20 parts of plant leaf protein, 1 part of compound essential oil, 0.2 part of sodium hypochlorite, 0.2 part of protease, 0.2 part of activated carbon, 1 part of nano antibacterial agent and 50 parts of purified water.
Preferably, the feed comprises the following raw materials in parts by weight: 20 parts of soybean protein, 40 parts of plant leaf protein, 10 parts of compound essential oil, 1 part of sodium hypochlorite, 1 part of protease, 1 part of activated carbon, 5 parts of nano antibacterial agent and 80 parts of purified water.
Preferably, the compound essential oil is arborvitae essential oil, mint essential oil and thyme essential oil according to a mass ratio of 2-4: 1: 1, mixing the mixture.
Preferably, the protease is an alkaline protease, an acid protease or a neutral protease.
Preferably, the nano antibacterial agent is a nano silver antibacterial agent, a nano titanium dioxide antibacterial agent or a nano zinc oxide antibacterial agent.
Preferably, the plant leaf protein is one or a mixture of two of alfalfa leaf protein and pumpkin leaf protein.
On the other hand, the invention also provides a preparation method of the compound formaldehyde scavenger containing the thuja essential oil, which comprises the following steps:
s1, preparing soybean protein and plant leaf protein into emulsion or suspension of water with concentration of 2-10%, performing heat treatment to denature the protein, and refining to break the protein particles to 0.1-20 μm;
s2, adding protease into the protein solution obtained in the S1, and performing countercurrent ultrasonic-assisted enzymatic hydrolysis to obtain a protein hydrolysate, wherein the parameters of the countercurrent ultrasonic-assisted enzymatic hydrolysis are 5-50kHz of ultrasonic frequency, 50-350W/L of power density, 20-60 ℃ of temperature, 2-10 of pH and 30-180min of ultrasonic-assisted enzymatic hydrolysis;
s3, sterilizing and cooling the protease hydrolysate obtained in the step S2, performing combined fermentation by using yeast, lactic acid bacteria and bacillus subtilis, performing reduced pressure vacuum-pumping fermentation to remove fishy smell, wherein the addition amount of the strain leaven is 105-1010Adding cfu/kg viable count; fermenting for 15-60min at 25-45 deg.C and stirring speed of 20-60 rpm; knotThe vacuum degree of the combined decompression and vacuumizing fermentation is 5 multiplied by 103-5×104pa；
S4, filtering the reaction solution obtained in the step S3 to obtain a clear solution, adding sodium hypochlorite to oxidize for 3-5 hours until the oxidation solution becomes bluish white, decoloring with active carbon at the temperature of 70-100 ℃ for 1.5-2.5 hours, filtering, and collecting filtrate;
s5, adding the compound essential oil, the nano antibacterial agent and the purified water into the filtrate obtained in the step S4, and uniformly stirring to obtain the compound formaldehyde scavenger containing the thuja essential oil.
Preferably, the conditions for heat treatment denaturation in S1 are as follows: heating at 100-135 deg.C for 5-15 min; the refining treatment is carried out by a high-pressure homogenizer at 10-30Mpa and 30-60 deg.C for 1-3 times to make the protein particle size to 0.1-20 μm.
Thuja (scientific name: Thujasumechuensis) is a plant of Thuja of Cupressaceae. As early as in Ben Cao gang mu, the magical efficacy of the herb is highly evaluated. The arborvitae is recorded to be a wood with multiple lives, can be taken, is non-toxic, can remove wind and dampness, can calm five internal organs, can moisten and beautify the color of a human body after being taken for a long time, can improve the hearing and eyesight, cannot hunger or age, can prolong life, moisten the liver, nourish heart-qi, moisten kidney dryness, calm soul and calm mind, benefit intelligence and tranquilize mind, and moisten hair. Based on the theory, researches show that root, stem and leaf of thuja contain terpenoids such as thujoram, alpha-pinene, and thyme, and the terpenoids have good lipid solubility and low molecular weight, and have the effects of resisting bacteria, resisting oxidation, resisting tumor, lowering blood pressure, expelling parasites, resisting inflammation, promoting urination, eliminating phlegm, and strengthening body. The thuja essential oil extracted from thuja has various biological activities, and can promote the increase of immune protein, effectively regulate the balance of respiration and central nervous system, enhance the immunity of human body, culture the vital qi of human body, and achieve the effects of treatment, body building and body building after being absorbed by human body.
The peppermint essential oil has double effects, and can cool when being hot and warm when being cold, so that the peppermint essential oil has excellent effect of treating cold, has good effect on symptoms generated by respiratory tract, and has certain curative effect on dry cough, asthma, bronchitis, pneumonia and phthisis. Is also beneficial to digestive tract diseases, and has the functions of eliminating flatulence, relieving stomachache and heartburn; in addition, it can relieve pain, is also effective in migraine, and can help to reduce fever. Menthol in the mint essential oil also has remarkable anti-tumor activity and can inhibit the growth of various tumor cells.
The thyme essential oil has the effects of resisting infection, stimulating leucocyte hyperplasia and blood circulation, reducing blood pressure, recovering vitality, enhancing cerebral function and the like, and the thymol contained in the thyme essential oil has the effects of sterilizing, resisting oxidation, eliminating phlegm, removing mites and the like, and particularly has obvious curative effects on oral and throat mucous membranes, dermatophytosis, radiation mycosis and otitis. In addition, thymol also shows good anti-tumor pharmacological activity, and thymol can induce the apoptosis of liver cancer cells, prostate cancer cells, colon cancer cells, leukemia cells and stomach cancer cells from aspects of mitochondrial oxidative stress, caspase, mitogen-activated protein kinase (MAPK), extracellular regulatory protein kinase (ERK), apoptosis genes Bax and Bcl-2 and the like, and plays an anti-cancer role through ways of reducing mitochondrial transmembrane potential, inducing the cells to generate ROS, enhancing the expression of caspase, down-regulating the expression of Bcl-2, increasing the phosphorylation of MAPK and ERK and the like.
Pumpkin, also called wheat melon, pumpkin, white gourd, etc, belongs to the cucurbitaceae family of pumpkin genus annual or perennial herb plants. The pumpkin has good cultivation characteristics, strong adaptability to environment, strong stress resistance and strong disease and pest resistance, various varieties, wide geographical distribution, extensive management resistance, low production cost, high yield and long storage life, is an important economic crop in China, has the annual yield of 410 ten thousand t and about 200 thousand t of stems and leaves, and accounts for 30 percent of the total yield of the world. At present, most resources of the stems and leaves of the pumpkins are wasted except for a small amount of the stems and leaves of the pumpkins which are eaten and used as feed, and researches show that the content of crude protein in the pumpkins leaves is about 30 percent, and if the pumpkins can be developed and utilized, the economic benefit generated by the pumpkins is very high.
The alfalfa serving as high-quality leguminous forage has the advantages of strong adaptability, multiple varieties, long cultivation history, wide distribution range, stable productivity, long utilization period, high nutritional value, good palatability, good livestock and poultry feeding effect and the like. In a new historical period, the development of various alfalfa products has been gaining attention, with alfalfa leaf protein concentrate being a popular product with superior amino acid content and composition over other vegetable proteins.
Compared with the prior art, the invention has the beneficial technical effects that:
the invention makes full use of the plant leaf protein (pumpkin leaf protein or alfalfa leaf protein) to replace part of the soybean protein, saves the cost and improves the economic benefit. Products obtained by carrying out enzymolysis, oxidation and other treatments on plant leaf protein and soybean protein in the invention contain a large amount of amino acid derivatives, biological enzymes and the like, amino groups in the amino acid derivatives react with formaldehyde to form hydroxymethyl derivatives of amino acids, the hydroxymethyl derivatives of amino acids have good chemical stability, are nontoxic, tasteless and nonvolatile, cannot cause secondary pollution, and the biological enzymes and the like play a role in removing formaldehyde mainly by adsorbing the formaldehyde; the thuja essential oil, the mint essential oil and the thyme essential oil in the invention can exert the functions of each component and have the synergistic effect, so that the increase of immune protein can be promoted, the balance of respiration and central nervous system can be effectively adjusted, the mucous membrane of oral cavity and throat can be protected, and the growth of various tumor cells can be inhibited; the nano antibacterial agent has strong antibacterial action, and can ensure the antibacterial effect for a long time. The invention adopts countercurrent ultrasonic to assist protease hydrolysis, can improve the hydrolysis degree and shorten the enzymatic hydrolysis time, and can further improve the effects of fermentation and deodorization and odor removal by adopting a decompression and vacuum-pumping fermentation mode.
Detailed Description
The following further describes the embodiments of the present invention. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto. In addition, the technical features involved in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
Example 1
A compound formaldehyde scavenger containing thuja essential oil comprises the following raw materials in parts by weight: 15 parts of soybean protein, 30 parts of pumpkin leaf protein, 5 parts of compound essential oil, 0.5 part of sodium hypochlorite, 0.5 part of neutral protease, 0.5 part of activated carbon, 3 parts of nano-silver antibacterial agent and 70 parts of purified water, wherein the compound essential oil is arborvitae essential oil, mint essential oil and thyme essential oil according to the mass ratio of 3: 1: 1, mixing the mixture.
The preparation method of the compound formaldehyde scavenger containing the thuja essential oil comprises the following steps:
s1, preparing an emulsion or suspension of 6% water from soybean protein and pumpkin leaf protein, and heating at 120 deg.C for 10 min; refining with high pressure homogenizer at 45 deg.C under 20Mpa for 2 times to obtain protein particles of 10 μm;
s2, adding neutral protease into the protein solution obtained in the S1, and performing countercurrent ultrasonic-assisted enzymatic hydrolysis to obtain a protein hydrolysate, wherein the parameters of the countercurrent ultrasonic-assisted enzymatic hydrolysis are ultrasonic frequency 25kHz, power density 200W/L, temperature 40 ℃, pH7 and ultrasonic-assisted enzymatic hydrolysis time 100 min;
s3, sterilizing and cooling the protease hydrolysate obtained in the step S2, performing combined fermentation by using yeast, lactic acid bacteria and bacillus subtilis, performing reduced pressure vacuum-pumping fermentation to remove fishy smell, wherein the addition amount of the strain leaven is 108Adding cfu/kg viable count; fermenting for 30min at 35 deg.C and stirring speed of 40 rpm; the vacuum degree of the fermentation is 5 multiplied by 10 in combination with decompression and vacuum pumping3pa；
S4, filtering the reaction solution obtained in the step S3 to obtain a clear solution, adding sodium hypochlorite to oxidize for 4 hours until the oxidation solution becomes bluish white, decolorizing with activated carbon, keeping the temperature at 80 ℃ for 2 hours, filtering, and collecting filtrate;
s5, adding the compound essential oil, the nano-silver antibacterial agent and the purified water into the filtrate obtained in the step S4, and uniformly stirring to obtain the compound formaldehyde scavenger containing the thuja essential oil.
Example 2
A compound formaldehyde scavenger containing thuja essential oil comprises the following raw materials in parts by weight: 10 parts of soybean protein, 20 parts of alfalfa leaf protein, 1 part of compound essential oil, 0.2 part of sodium hypochlorite, 0.2 part of acid protease, 0.2 part of activated carbon, 1 part of nano titanium dioxide antibacterial agent and 50 parts of purified water, wherein the compound essential oil is arborvitae essential oil, mint essential oil and thyme essential oil according to the mass ratio of 2: 1: 1, mixing the mixture.
The preparation method of the compound formaldehyde scavenger containing the thuja essential oil comprises the following steps:
s1, preparing soybean protein and alfalfa leaf protein into 2% water emulsion or suspension, and heating at 100 deg.C for 5 min; refining with high pressure homogenizer at 10Mpa at 30 deg.C for 1 times to obtain protein particles of 1 μm;
s2, adding acid protease into the protein solution obtained in the S1, and performing countercurrent ultrasonic-assisted enzymatic hydrolysis to obtain a protein hydrolysate, wherein the parameters of the countercurrent ultrasonic-assisted enzymatic hydrolysis are ultrasonic frequency of 5kHz, power density of 50W/L, temperature of 20 ℃, pH of 2, and ultrasonic-assisted enzymatic hydrolysis time of 30 min;
s3, sterilizing and cooling the protease hydrolysate obtained in the step S2, performing combined fermentation by using yeast, lactic acid bacteria and bacillus subtilis, performing reduced pressure vacuum-pumping fermentation to remove fishy smell, wherein the addition amount of the strain leaven is 105Adding cfu/kg viable count; fermenting for 15min at 25 deg.C and stirring speed of 20 rpm; the vacuum degree of the fermentation is 5 multiplied by 10 in combination with decompression and vacuum pumping4pa；
S4, filtering the reaction solution obtained in the step S3 to obtain a clear solution, adding sodium hypochlorite to oxidize for 3 hours until the oxidation solution becomes bluish white, decoloring with activated carbon at 70 ℃ for 1.5 hours, filtering, and collecting filtrate;
s5, adding the compound essential oil, the nano titanium dioxide antibacterial agent and the purified water into the filtrate obtained in the step S4, and uniformly stirring to obtain the compound formaldehyde scavenger containing the thuja essential oil.
Example 3
A compound formaldehyde scavenger containing thuja essential oil comprises the following raw materials in parts by weight: 20 parts of soybean protein, 40 parts of pumpkin leaf protein, 10 parts of compound essential oil, 1 part of sodium hypochlorite, 1 part of alkaline protease, 1 part of activated carbon, 5 parts of nano zinc oxide antibacterial agent and 80 parts of purified water, wherein the compound essential oil is arborvitae essential oil, mint essential oil and thyme essential oil according to the mass ratio of 4: 1: 1, mixing the mixture.
The preparation method of the compound formaldehyde scavenger containing the thuja essential oil comprises the following steps:
s1, preparing 10% water emulsion or suspension from soybean protein and pumpkin leaf protein, and heating at 135 deg.C for 15 min; refining with high pressure homogenizer at 60 deg.C under 30Mpa for 3 times to obtain protein particles of 20 μm;
s2, adding alkaline protease into the protein solution obtained in the S1, and performing countercurrent ultrasonic-assisted enzymatic hydrolysis to obtain a protein hydrolysate, wherein the parameters of the countercurrent ultrasonic-assisted enzymatic hydrolysis are ultrasonic frequency 50kHz, power density 350W/L, temperature 60 ℃, pH10 and ultrasonic-assisted enzymatic hydrolysis time 180 min;
s3, sterilizing and cooling the protease hydrolysate obtained in the step S2, performing combined fermentation by using yeast, lactic acid bacteria and bacillus subtilis, performing reduced pressure vacuum-pumping fermentation to remove fishy smell, wherein the addition amount of the strain leaven is 1010Adding cfu/kg viable count; the fermentation time is 60min, the fermentation temperature is 45 ℃, and the stirring speed is 60 rpm; the vacuum degree of the fermentation is 5 multiplied by 10 in combination with decompression and vacuum pumping4pa；
S4, filtering the reaction solution obtained in the step S3 to obtain a clear solution, adding sodium hypochlorite to oxidize for 5 hours until the oxidation solution becomes bluish white, decoloring with activated carbon, keeping the temperature at 100 ℃ for 2.5 hours, filtering, and collecting filtrate;
s5, adding the compound essential oil, the nano zinc oxide antibacterial agent and the purified water into the filtrate obtained in the step S4, and uniformly stirring to obtain the compound formaldehyde scavenger containing the thuja essential oil.
Example 4
A compound formaldehyde scavenger containing thuja essential oil comprises the following raw materials in parts by weight: 15 parts of soybean protein, 15 parts of pumpkin leaf protein, 15 parts of alfalfa leaf protein, 5 parts of compound essential oil, 0.5 part of sodium hypochlorite, 0.5 part of neutral protease, 0.5 part of active carbon, 3 parts of nano-silver antibacterial agent and 70 parts of purified water, wherein the compound essential oil is arborvitae essential oil, mint essential oil and thyme essential oil according to the mass ratio of (3): 1: 1, mixing the mixture.
The preparation method of the compound formaldehyde scavenger containing the thuja essential oil comprises the following steps:
s1, preparing an emulsion or suspension of 6% water from soybean protein and pumpkin leaf protein, and heating at 120 deg.C for 10 min; refining with high pressure homogenizer at 45 deg.C under 20Mpa for 2 times to obtain protein particles of 10 μm;
s2, adding neutral protease into the protein solution obtained in the S1, and performing countercurrent ultrasonic-assisted enzymatic hydrolysis to obtain a protein hydrolysate, wherein the parameters of the countercurrent ultrasonic-assisted enzymatic hydrolysis are ultrasonic frequency 25kHz, power density 200W/L, temperature 40 ℃, pH7 and ultrasonic-assisted enzymatic hydrolysis time 100 min;
s3, sterilizing and cooling the protease hydrolysate obtained in the step S2, performing combined fermentation by using yeast, lactic acid bacteria and bacillus subtilis, performing reduced pressure vacuum-pumping fermentation to remove fishy smell, wherein the addition amount of the strain leaven is 108Adding cfu/kg viable count; fermenting for 30min at 35 deg.C and stirring speed of 40 rpm; the vacuum degree of the fermentation is 5 multiplied by 10 in combination with decompression and vacuum pumping3pa；
S4, filtering the reaction solution obtained in the step S3 to obtain a clear solution, adding sodium hypochlorite to oxidize for 4 hours until the oxidation solution becomes bluish white, decolorizing with activated carbon, keeping the temperature at 80 ℃ for 2 hours, filtering, and collecting filtrate;
s5, adding the compound essential oil, the nano-silver antibacterial agent and the purified water into the filtrate obtained in the step S4, and uniformly stirring to obtain the compound formaldehyde scavenger containing the thuja essential oil.
And (3) performance testing:
1. physical and chemical index tests were conducted using the composite formaldehyde scavengers obtained in examples 1 to 4 as samples.
Sensory testing: visually observing the properties of the product to see whether the product is abnormal or not;
and (3) heat resistance test: putting the sample into an electric heating constant temperature incubator at the temperature of (40 +/-1) DEG C for 24 hours, and observing whether phenomena such as thinning, discoloration, layering, hardness change and the like exist after the temperature is recovered to judge the heat resistance of the sample;
cold resistance test: putting the sample into a refrigerator at the temperature of minus 5 to minus 10 +/-1 ℃ for 24 hours, and observing whether phenomena such as thinning, color change, layering, hardness change and the like exist after the room temperature is recovered to judge the cold resistance of the sample;
the results are shown in Table 1
TABLE 1 results of physical and chemical tests
| Examples | Sensory testing | Heat resistance test | Cold resistance test |
| Example 1 | No abnormality | Stabilization | Stabilization |
| Example 2 | No abnormality | Stabilization | Stabilization |
| Example 3 | No abnormality | Stabilization | Stabilization |
| Example 4 | No abnormality | Stabilization | Stabilization |
2. And (3) safety testing: referring to the GB/T16886.5 cytotoxicity test, the cytotoxicity of the examples 1 to 4 is 0 grade.
3. And (3) testing formaldehyde removal performance:
(1) formaldehyde removal at Room temperature test (25 ℃ C.)
And (4) constructing an experimental device according to the national standard QB-T2761-2006 method for measuring the purification effect of indoor air purification products, and carrying out experimental tests. A floor of 1 square meter capable of releasing formaldehyde was placed in a 10 square meter empty chamber, the concentration of formaldehyde in the empty chamber was measured after 24 hours, the products of examples 1 to 4 were respectively and uniformly sprayed in each empty chamber, and a light source of 1W power was placed in the empty chamber, and the concentration of formaldehyde in the empty chamber was measured after 12 hours. The formaldehyde removal rate (initial concentration-sample concentration) × 100/initial concentration, and the test results are shown in table 2.
(2) High temperature Formaldehyde removal Performance test (100 ℃ C.)
The temperature of the blank chamber was raised to 100 ℃ and the above test was repeated.
TABLE 2 Formaldehyde removal test results
| Examples | Formaldehyde removal rate (25 ℃ C.) | Formaldehyde removal rate (100 ℃ C.) |
| Example 1 | 100％ | 97％ |
| Example 2 | 97％ | 95％ |
| Example 3 | 96％ | 94％ |
| Example 4 | 97％ | 96％ |
As can be seen from the data in the table 2, the products prepared in the embodiments 1 to 4 of the present invention can effectively remove formaldehyde in the same space, and the removal rate reaches more than 96% at 25 ℃, which indicates that the compound formaldehyde scavenger containing thuja essential oil of the present invention can effectively remove formaldehyde harmful gas and odor generated by decoration of new residence, and well solve the problem of indoor air pollution.
The embodiments of the present invention have been described in detail, but the present invention is not limited to the described embodiments. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, and the scope of protection is still within the scope of the invention.
Claims (10)
1. The compound formaldehyde scavenger containing thuja essential oil is characterized by comprising the following raw materials in parts by weight: 10-20 parts of soybean protein, 20-40 parts of plant leaf protein, 1-10 parts of compound essential oil, 0.2-1 part of sodium hypochlorite, 0.2-1 part of protease, 0.2-1 part of active carbon, 1-5 parts of nano antibacterial agent and 50-80 parts of purified water.
2. The compound formaldehyde scavenger containing thuja essential oil according to claim 1, which is characterized by comprising the following raw materials in parts by weight: 15 parts of soybean protein, 30 parts of plant leaf protein, 5 parts of compound essential oil, 0.5 part of sodium hypochlorite, 0.5 part of protease, 0.5 part of activated carbon, 3 parts of nano antibacterial agent and 70 parts of purified water.
3. The compound formaldehyde scavenger containing thuja essential oil according to claim 1, which is characterized by comprising the following raw materials in parts by weight: 10 parts of soybean protein, 20 parts of plant leaf protein, 1 part of compound essential oil, 0.2 part of sodium hypochlorite, 0.2 part of protease, 0.2 part of activated carbon, 1 part of nano antibacterial agent and 50 parts of purified water.
4. The compound formaldehyde scavenger containing thuja essential oil according to claim 1, which is characterized by comprising the following raw materials in parts by weight: 20 parts of soybean protein, 40 parts of plant leaf protein, 10 parts of compound essential oil, 1 part of sodium hypochlorite, 1 part of protease, 1 part of activated carbon, 5 parts of nano antibacterial agent and 80 parts of purified water.
5. The compound formaldehyde scavenger containing arborvitae essential oil according to claim 1, wherein the compound essential oil is arborvitae essential oil, mint essential oil and thyme essential oil according to a mass ratio of 2-4: 1: 1, mixing the mixture.
6. The compound formaldehyde scavenger containing thuja essential oil according to claim 1, wherein the protease is alkaline protease, acid protease or neutral protease.
7. The compound formaldehyde scavenger containing thuja essential oil according to claim 1, wherein the nano antibacterial agent is a nano silver antibacterial agent, a nano titanium dioxide antibacterial agent or a nano zinc oxide antibacterial agent.
8. The compound formaldehyde scavenger containing thuja essential oil according to claim 1, wherein the plant leaf protein is one or a mixture of two of alfalfa leaf protein and pumpkin leaf protein.
9. The preparation method of the compound formaldehyde scavenger containing the thuja essential oil according to any one of claims 1 to 8, which is characterized by comprising the following steps:
s1, preparing soybean protein and plant leaf protein into emulsion or suspension of water with concentration of 2-10%, performing heat treatment to denature the protein, and refining to break the protein particles to 0.1-20 μm;
s2, adding protease into the protein solution obtained in the S1, and performing countercurrent ultrasonic-assisted enzymatic hydrolysis to obtain a protein hydrolysate, wherein the parameters of the countercurrent ultrasonic-assisted enzymatic hydrolysis are 5-50kHz of ultrasonic frequency, 50-350W/L of power density, 20-60 ℃ of temperature, 2-10 of pH and 30-180min of ultrasonic-assisted enzymatic hydrolysis;
s3, sterilizing and cooling the protease hydrolysate obtained in the step S2, performing combined fermentation by using yeast, lactic acid bacteria and bacillus subtilis, performing reduced pressure vacuum-pumping fermentation to remove fishy smell, wherein the addition amount of the strain leaven is 105-1010Adding cfu/kg viable count; fermenting for 15-60min at 25-45 deg.C and stirring speed of 20-60 rpm; the vacuum degree of the fermentation is 5 multiplied by 10 in combination with decompression and vacuum pumping3-5×104pa；
S4, filtering the reaction solution obtained in the step S3 to obtain a clear solution, adding sodium hypochlorite to oxidize for 3-5 hours until the oxidation solution becomes bluish white, decoloring with active carbon at the temperature of 70-100 ℃ for 1.5-2.5 hours, filtering, and collecting filtrate;
s5, adding the compound essential oil, the nano antibacterial agent and the purified water into the filtrate obtained in the step S4, and uniformly stirring to obtain the compound formaldehyde scavenger containing the thuja essential oil.
10. The method for preparing the compound formaldehyde scavenger containing thuja essential oil according to claim 9, wherein the heat treatment denaturation conditions in S1 are as follows: heating at 100-135 deg.C for 5-15 min; the refining treatment is carried out by a high-pressure homogenizer at 10-30Mpa and 30-60 deg.C for 1-3 times to make the protein particle size to 0.1-20 μm.
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| CN112295401A (en) * | 2020-12-29 | 2021-02-02 | 南京凯创协同纳米技术有限公司 | Preparation method of spray for removing fishy smell of farmer market |
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