CN110639710A

CN110639710A - Filter tube centrifuge for research of nucleic acid aptamer

Info

Publication number: CN110639710A
Application number: CN201910890846.XA
Authority: CN
Inventors: 张李钰; 武海滨; 王猛; 杨颖�; 车凤玉; 王国霞; 李俏
Original assignee: Xian Childrens Hospital
Current assignee: Xian Childrens Hospital
Priority date: 2019-09-20
Filing date: 2019-09-20
Publication date: 2020-01-03

Abstract

The invention relates to and discloses a filter tube centrifuge for researching a nucleic acid aptamer, which comprises a case, wherein the top of the case is provided with an inlet, one end of the inlet, which is far away from the case, is communicated with a first control valve, one end of the first control valve, which is far away from the inlet, is communicated with a material box, and the top of the case, which is positioned on the right side of the inlet, is provided with a first through hole. This chimney filter is used in aptamer research, through starter motor one, drive the rotary drum and rotate, and then it flows to the upper portion export on right side to drive the object that upper portion density is little, lower part liquid then can flow through the lower part export, the target of liquid separation has been realized, through the fixed of transmission shaft one with the bearing, the supporting role of the object that has reached the rotary drum and carry out direct or indirect connection with the rotary drum, the rotation of rotary drum has been guaranteed simultaneously, the spherical displacement that has restricted transmission shaft two and not restricted the angular displacement in bottom through the recess, make the upper portion export increase relatively through setting up of chamber door, thereby work efficiency has been improved.

Description

Filter tube centrifuge for research of nucleic acid aptamer

Technical Field

The invention relates to the technical field of pure steam generators, in particular to a filter tube centrifuge for researching a nucleic acid aptamer.

Background

The general two exports of filter tube centrifuge for aptamer research on the current market are all realized controlling through the control valve, because the interface of control valve is relevant with the export size to the control of the degree of the size of export has been restricted, thereby has restricted centrifuge's work efficiency, if too big with the control valve preparation, then will increase cost.

Disclosure of Invention

The invention aims to provide a filter tube centrifuge for researching a nucleic acid aptamer, and the aim of improving the working efficiency is fulfilled.

In order to achieve the purpose of improving the working efficiency, the invention provides the following technical scheme: a filter tube centrifuge for nucleic acid aptamer research comprises a case, wherein an inlet is formed in the top of the case, one end, far away from the case, of the inlet is communicated with a first control valve, one end, far away from the inlet, of the first control valve is communicated with a material box, a first through hole is formed in the top of the case and located on the right side of the inlet, a support frame is fixedly connected to the top of the case and located above the first through hole through screws, a first motor is fixedly connected to the bottom of a top plate of the support frame and located above the first through hole through screws, a first transmission shaft is fixedly connected to the output end of the first motor, a rotary drum is sleeved on the cylindrical surface of the first transmission shaft, a first fixing ring is sleeved on the cylindrical surface of the first transmission shaft and located on the inner ring of the rotary drum, the rotary drum is fixedly connected to the, the end, away from the right side inner wall of the case, of the inner support plate in the case is fixedly welded with a ball bearing, the cylindrical surface, away from the drum, of the transmission shaft I is sleeved on an inner ring of the ball bearing, the cylindrical surface, away from the drum, of the transmission shaft I is positioned on the inner ring of the drum, the cylindrical surface, away from the drum, of the transmission shaft I is sleeved on an inner ring of the ball bearing, the cylindrical surface, positioned on the transmission shaft I, of the transmission shaft I is sleeved on a fixed ring II, the transmission shaft I is fixedly connected with the ball bearing through the fixed ring II, the right wall of the case is provided with an upper outlet, the right side outer wall of the case is fixedly connected with a hinge through a screw right behind the upper outlet, the right side outer wall of the case is movably hinged with a case door through a hinge, the inner wall of the second through hole is sleeved with a second transmission shaft, the second transmission shaft penetrates through the second through hole and extends to a second motor fixedly connected above the upper supporting plate, the second motor is fixedly connected with the top of the upper supporting plate through screws, a lower supporting plate is fixedly welded to the front face of the case and is located right below the upper supporting plate, a groove is formed in the top of the lower supporting plate, the bottom of the second transmission shaft is connected to the bottom of the groove in a rolling mode, the cylindrical surfaces, which are recessed into the groove, of the second transmission shaft are tied to ropes, one end, away from the second transmission shaft, of each rope is tied to a fixed block, a lower outlet is formed in the top of the case, one end, away from the case, of the lower outlet is communicated with a second control valve.

Preferably, the blades on the rotary drum are one centimeter thick, the number of the blades is six, and the bottom of the rotary drum is located at a position lower than the bottom of the outlet of the upper object.

Preferably, a projection is arranged on the end face close to the inside of the case after the case door is closed, and the projection is matched with the upper outlet.

Preferably, the sealing ring is made of super-elastic material, and the sealing ring is sleeved on the lug on the box door and then forms sealing with the box door.

Preferably, the groove is formed by superposing a cylinder and a hemisphere, the upper part of the groove is cylindrical, the lower part of the groove is a sphere, and the position of the groove corresponds to that of the second through hole.

Preferably, the shape of the second transmission shaft is two-section, and the lower end surface of the second transmission shaft is spherical matched with the groove.

Preferably, the number of the fixed blocks is two, holes are formed in the same positions of the two fixed blocks, and the rope penetrates through the holes to be knotted and fixed with the fixed blocks.

The invention provides a filter tube centrifuge for researching a nucleic acid aptamer. The method has the following beneficial effects: through starter motor one, drive the rotary drum and rotate, and then drive the upper portion export outflow of the little object of upper portion density to the right side, lower part liquid then can flow through the lower part export, the target of liquid separation has been realized, through transmission shaft one with ball bearing's fixed, the supporting role of the object that carries out direct or indirect connection to the rotary drum and with the rotary drum has been reached, the rotation of rotary drum has been guaranteed simultaneously, the spherical displacement that has restricted transmission shaft two of bottom through the recess does not restrict angular displacement, make the upper portion export increase relatively through the setting of chamber door, thereby work efficiency has been improved.

Drawings

FIG. 1 is a schematic view of the present invention;

FIG. 2 is a right side view of the present invention;

FIG. 3 is a top view of the present invention;

fig. 4 is an enlarged view of the invention at a in fig. 1.

In the figure: the device comprises a case 1, an inlet 2, a control valve I3, a material box 4, a through hole I5, an upper outlet 6, a support frame 7, a motor I8, an in-box support plate 9, a ball bearing 10, a fixed ring II 11, a hinge 12, a box door 13, a sealing ring 14, a fixed ring I15, a rotating cylinder 16, a transmission shaft I17, a lower outlet 18, a control valve II 19, a water outlet pipe 20, an upper support plate 21, a through hole II 22, a motor II 23, a groove 24, a lower support plate 25, a rope 26, a fixed block 27 and a transmission shaft II 28.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the invention and to simplify the description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are therefore not to be considered limiting of the invention.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

Referring to fig. 1-4, the present invention provides a technical solution: a filter tube centrifuge for researching nucleic acid aptamers comprises a case 1, wherein the top of the case 1 is provided with an inlet 2, one end of the inlet 2, far away from the case 1, is communicated with a control valve I3, one end of the control valve I3, far away from the inlet 2, is communicated with a material box 4, the top of the case 1, which is positioned at the right side of the inlet 2, is provided with a through hole I5, the top of the case 1, which is positioned at the upper part of the through hole I5, is fixedly connected with a support frame 7 through a screw, the bottom of the top plate of the support frame 7, which is positioned at the upper part of the through hole I5, is fixedly connected with a motor I8, a transmission shaft I17 is driven to rotate by starting the motor I8, so as to drive a rotary drum 16 to rotate, further drive objects with small upper density to flow out to an upper outlet 6 at the right side, the supporting effect on the rotary drum 16 and objects directly or indirectly connected with the rotary drum 16 is achieved through the fixation of the first transmission shaft 17 and the ball bearings 10, the rotation of the rotary drum 16 is guaranteed, the rotary drum 16 is sleeved on the cylindrical surface of the first transmission shaft 17, the fan blades on the rotary drum 16 are one centimeter in thickness, the number of the fan blades is six, the bottom of the rotary drum 16 is located at a position lower than the bottom surface of an outlet of an upper object, a first fixing ring 15 is sleeved on the cylindrical surface of the first transmission shaft 17 and located at the inner ring of the rotary drum 16, the rotary drum 16 is fixedly connected to the cylindrical surface of the first transmission shaft 17 through the first fixing ring 15, an in-box supporting plate 9 is fixedly welded on the inner wall of the right side of the case 1, the ball bearings 10 are fixedly welded on one end, far away from the inner wall of the right side of the case 1, of the, a fixing ring II 11 is sleeved on the cylindrical surface of a transmission shaft I17 and positioned at the inner ring of the ball bearing 10, the transmission shaft I17 is fixedly connected with the ball bearing 10 through the fixing ring II 11, an upper outlet 6 is arranged on the right wall of the case 1, a hinge 12 is fixedly connected with the right outer wall of the case 1 and positioned right behind the upper outlet 6 through a screw, a case door 13 is movably hinged on the right outer wall of the case 1 through the hinge, a bump is arranged on the end surface close to the inside of the case 1 after the case door 13 is closed, the bump is matched with the upper outlet 6, a sealing ring 14 is fixedly sleeved on the end surface bump close to the inside of the case 1 after the case door 13 is closed, the sealing ring 14 is made of super-elastic material, the sealing ring 14 is sleeved on the bump on the case door 13 to form sealing with the case door 13, two mutually symmetrical fixing blocks 27, the same positions of the two fixing blocks 27 are provided with holes, ropes are knotted through the holes to be fixed with the fixing blocks 27, the front of the case 1 is fixedly welded with an upper supporting plate 21, the top of the upper supporting plate 21 is provided with a through hole II 22, the inner wall of the through hole II 22 is sleeved with a transmission shaft II 28, the transmission shaft II 28 penetrates through the through hole II 22 and extends to the upper part of the upper supporting plate 21 to be fixedly connected with a motor II 23, the motor II 23 is fixedly connected with the top of the upper supporting plate 21 through screws, the transmission shaft II 28 is in a two-section shape, the lower end face of the transmission shaft II 28 is in a spherical shape matched with the groove 24, a lower supporting plate 25 is fixedly welded on the front of the case 1 and is positioned under the upper supporting plate 25, the top of the lower supporting plate 25 is provided with a groove 24, the displacement of the transmission shaft II 28 is, thereby work efficiency has been improved, the shape of recess 24 is cylindrical and hemisphere stack forms, upper portion is cylindrical, the lower part is the ball, and the position of recess 24 corresponds with the position of through-hole two 22, the bottom roll connection of transmission shaft two 28 is in recess 24 bottom, two go up the face of cylinder that the recess was gone up on transmission shaft two 28 have been tied and have been connect rope 26, the one end that transmission shaft two 28 was kept away from to rope 26 is tied and is connect on fixed block 27, lower part export 18 has been seted up at the top of machine case 1, the one end intercommunication that machine case 1 was kept away from to lower part export 18 has control valve two 19, the one end intercommunication that lower part export 18 was kept away from to control.

The working principle is as follows: during the use, through the starter motor 8, drive transmission shaft 17 and rotate, thereby drive rotary drum 16 and carry out the separation work, through the starter motor two 23, drive transmission shaft two 28 and rotate, and then drive rope 26 round joint on transmission shaft two 28, thereby strain chamber door 13 to with the cooperation of upper portion export 6, realize sealedly, the addition of quilt separation thing can be controlled through control valve one 3, can control the outflow speed and the outflow state of lower part quilt separation thing through control valve two 19, restrict transmission shaft one 17 displacement downwards through ball bearing 10, restrict transmission shaft two 28 displacement downwards and do not restrict angular displacement through recess 24.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a filter tube centrifuge is used in research of aptamer, includes quick-witted case (1), its characterized in that: the top of the case (1) is provided with an inlet (2), one end, far away from the case (1), of the inlet (2) is communicated with a first control valve (3), one end, far away from the inlet (2), of the first control valve (3) is communicated with a material box (4), the top of the case (1) and positioned on the right side of the inlet (2) are provided with a first through hole (5), the top of the case (1) and positioned above the first through hole (5) are fixedly connected with a support frame (7) through screws, the bottom of a top plate of the support frame (7) and positioned above the first through hole (5) are fixedly connected with a first motor (8), an output end of the first motor (8) is fixedly connected with a first transmission shaft (17), a cylindrical surface of the first transmission shaft (17) is sleeved with a rotary drum (16), and an inner ring of the cylindrical surface of the first transmission shaft (17) and positioned on the, the rotating cylinder (16) is fixedly connected to the cylindrical surface of the first transmission shaft (17) through a first fixing ring (15), an in-box supporting plate (9) is fixedly welded on the right inner wall of the case (1), a ball bearing (10) is fixedly welded at one end, far away from the right inner wall of the case (1), of the in-box supporting plate (9), the first transmission shaft (17) is far away from the rotating cylinder (16) and is positioned at the cylindrical surface of the rotating cylinder (16) and is sleeved on an inner ring of the ball bearing (10), a second fixing ring (11) is sleeved on the cylindrical surface of the first transmission shaft (17) and is positioned at the inner ring of the ball bearing (10), the first transmission shaft (17) is fixedly connected with the ball bearing (10) through the second fixing ring (11), an upper outlet (6) is formed in the right wall of the case (1), a hinge (12) is fixedly connected with a screw right behind the right outer wall of, the right side outer wall of the case (1) is movably hinged with a case door (13) through a hinge, a sealing ring (14) is fixedly sleeved on an end face convex block close to the inside of the case (1) after the case door (13) is closed, two mutually symmetrical fixed blocks (27) are welded in the up-down direction of one end, far away from the hinge (12), of the case door (13), an upper supporting plate (21) is fixedly welded in the front of the case (1), a second through hole (22) is formed in the top of the upper supporting plate (21), a second transmission shaft (28) is sleeved on the inner wall of the second through hole (22), the second transmission shaft (28) penetrates through the second through hole (22) and extends to the upper portion supporting plate (21), a second motor (23) is fixedly connected with the top of the second motor (23) through a screw, a lower supporting plate (25) is fixedly welded in the front of the case (1) and is positioned, the top of the lower supporting plate (25) is provided with a groove (24), the bottom of the second transmission shaft (28) is connected to the bottom of the groove (24) in a rolling mode, two upper concave cylindrical surfaces of the second transmission shaft (28) are connected with ropes (26), one end, far away from the second transmission shaft (28), of each rope (26) is connected to a fixed block (27) in a fastening mode, the top of the case (1) is provided with a lower outlet (18), one end, far away from the case (1), of the lower outlet (18) is communicated with a second control valve (19), and one end, far away from the lower outlet (18), of the second control valve (19) is communicated with a water outlet pipe (20).

2. The aptamer-based research cartridge centrifuge according to claim 1, wherein: the fan blades are arranged on the rotary drum (16), the thickness of each fan blade is one centimeter, the number of the fan blades is six, and the position of the bottom of the rotary drum (16) is larger than the bottom surface of the outlet of the upper object.

3. The aptamer-based research cartridge centrifuge according to claim 1, wherein: and a convex block is arranged on the end surface close to the inside of the case (1) after the case door (13) is closed, and the convex block is matched with the upper outlet (6).

4. The aptamer-based research cartridge centrifuge according to claim 1, wherein: the sealing ring (14) is made of super-elastic materials, and the sealing ring (14) is sleeved on the convex block on the box door (13) and then forms sealing with the box door (13).

5. The aptamer-based research cartridge centrifuge according to claim 1, wherein: the groove (24) is formed by superposing a cylinder and a hemisphere, the upper part is cylindrical, the lower part is a sphere, and the position of the groove (24) corresponds to the position of the second through hole (22).

6. The aptamer-based research cartridge centrifuge according to claim 1, wherein: the shape of the second transmission shaft (28) is two-section, and the lower end face of the second transmission shaft (28) is spherical matched with the groove (24).

7. The aptamer-based research cartridge centrifuge according to claim 1, wherein: the number of the fixing blocks (27) is two, holes are formed in the same positions of the two fixing blocks (27), and the rope penetrates through the holes to be knotted and fixed with the fixing blocks (27).