CN110639638B - Kit for pretreating blood sample and using method thereof - Google Patents
Kit for pretreating blood sample and using method thereof Download PDFInfo
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- CN110639638B CN110639638B CN201910912117.XA CN201910912117A CN110639638B CN 110639638 B CN110639638 B CN 110639638B CN 201910912117 A CN201910912117 A CN 201910912117A CN 110639638 B CN110639638 B CN 110639638B
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- placing plate
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/06—Test-tube stands; Test-tube holders
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
- B01F31/20—Mixing the contents of independent containers, e.g. test tubes
- B01F31/26—Mixing the contents of independent containers, e.g. test tubes the containers being submitted to a wobbling movement
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
- B01F31/70—Drives therefor, e.g. crank mechanisms
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/30—Driving arrangements; Transmissions; Couplings; Brakes
- B01F35/32—Driving arrangements
- B01F35/32005—Type of drive
- B01F35/3202—Hand driven
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/30—Driving arrangements; Transmissions; Couplings; Brakes
- B01F35/33—Transmissions; Means for modifying the speed or direction of rotation
- B01F35/332—Transmissions; Means for modifying the speed or direction of rotation alternately changing the direction of rotation
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L1/00—Enclosures; Chambers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
- B01F2101/23—Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
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- General Health & Medical Sciences (AREA)
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Abstract
The invention discloses a kit for pretreating a blood sample and a using method thereof, and particularly relates to the technical field of kits, wherein the kit comprises a box main body, a rotating shaft is movably arranged in the box main body, a test tube placing plate is arranged at the top of the rotating shaft, rotating blocks are fixedly arranged on two sides of the test tube placing plate, and the rotating blocks are movably connected with the rotating shaft; the fixed operating support that is provided with in test tube placing plate surface, the operating support shape sets up to the U font, and the vertical downward setting in both ends, the fixed test tube layer board that is provided with in operating support bottom. According to the test tube placing plate, the selective transmission connection between the test tube placing plate and the rotating shaft is realized through the plug-in clamping connection of the connecting clamping columns and the connecting clamping holes, so that the test tubes placed on the test tube placing plate can be conveniently controlled to oscillate, the sample test tubes on other test tube placing plates are not influenced, the specific sample test tubes can be conveniently preprocessed, and the test efficiency is improved.
Description
Technical Field
The invention relates to the technical field of kits, in particular to a kit for pretreating a blood sample and a using method thereof.
Background
The pretreatment of the blood sample refers to the extraction of the analyte from the blood sample. The content, components and other parameters of the substance to be detected are detected by high performance liquid chromatography subsequently, so as to achieve the purposes of disease examination and health examination, and the pretreatment process of the blood sample generally comprises the following steps: adding solution A and plasma into a test tube, wherein the solution A is used for separating hybrid protein in the plasma; and (3) vortexing to fully mix the solution A in the test tube with the plasma, wherein the impure protein in the plasma forms floccules, and the floccules are formed by extraction.
However, in the using process of the existing kit, because the inside of the kit can contain a plurality of sample test tubes, all the sample test tubes are not necessary to be pretreated, so that the sample test tubes to be pretreated are inconvenient to distinguish from the sample test tubes which are not pretreated, the pretreatment operation of the sample test tubes is inconvenient, and the test is further influenced.
Disclosure of Invention
In order to overcome the above defects in the prior art, embodiments of the present invention provide a kit for blood sample pretreatment and a method for using the same, in which a connecting clip column is inserted into a connecting clip hole to be connected with a rotating shaft in a plug-in manner, so that a test tube placing plate is selectively connected to the rotating shaft in a transmission manner, and further, the test tube placing plate is controlled to oscillate, sample test tubes on other test tube placing plates are not affected, and a specific sample test tube is pretreated conveniently, so that test efficiency is improved, and the problems in the background art are solved.
In order to achieve the purpose, the invention provides the following technical scheme: a kit for pretreating a blood sample comprises a box main body, wherein a rotating shaft is movably arranged in the box main body, a test tube placing plate is arranged at the top of the rotating shaft, rotating blocks are fixedly arranged on two sides of the test tube placing plate, the rotating blocks are movably connected with the rotating shaft, the number of the test tube placing plates is seven, the seven test tube placing plates are sequentially arranged at the top of the rotating shaft, and the seven test tube placing plates and the rotating shaft are arranged in a cross manner;
the test tube placing plate is characterized in that an operation support is fixedly arranged on the surface of the test tube placing plate, the operation support is in a U-shaped shape, two ends of the operation support are vertically arranged downwards, a test tube supporting plate is fixedly arranged at the bottom of the operation support, test tube placing holes are formed in two ends of the surface of the test tube placing plate, grooves are formed in two ends of the surface of the test tube supporting plate, the test tube placing holes and the grooves are arranged in a vertical collinear manner, and sample test tubes are placed between the test tube placing holes and;
the test tube placing rack is characterized in that a movable hole is fixedly formed in the middle of the test tube placing rack, a rubber ring is fixedly sleeved in the movable hole, a lifting clamping column is movably arranged in the rubber ring, a connecting clamping hole is fixedly formed in the surface of the rotating shaft, the lifting clamping column is movably clamped with the connecting clamping hole, a U-shaped clamping sleeve is fixedly arranged at the top end of the lifting clamping column, and the U-shaped clamping sleeve is fastened with an operating support;
box main part one end activity is provided with the drive shaft, the vertical collineation setting of drive shaft and axis of rotation, axis of rotation one end and drive shaft one end are all fixed to be provided with and rotate the wheel, two it all sets up the fixed latch that is provided with, two to rotate the wheel surface third it sets up through latch meshing, the fixed movable positioning pole that is provided with in inside one side of box main part, movable positioning pole surface cover is equipped with a movable section of thick bamboo, it is provided with the rotation support to rotate wheel one side, rotate support top and axis of rotation fixed connection, rotate support bottom and movable section of thick bamboo and fixed connection, movable section of thick bamboo both ends all are provided with reset spring, reset spring cup joints with movable positioning pole.
In a preferred embodiment, the cross-sectional shape of the box body is trapezoidal, the top of the box body is provided with an opening, a box cover is hinged to one side of the surface of the box body, test tube compression cushion blocks are fixedly arranged on two sides inside the box cover, and the test tube compression cushion blocks are matched with the tops of the test tube placing plates.
In a preferred embodiment, the operation bracket is made of stainless steel material, the part of the operation bracket arranged at the bottom of the test tube placing plate is larger than the part arranged at the top of the test tube placing plate, and the shock absorption rubber ring is fixedly sleeved inside the test tube placing hole.
In a preferred embodiment, the lifting clamping column is made of a metal material, the operating bracket and the lifting clamping column are arranged on the same vertical plane, the U-shaped cutting sleeve is made of an elastic metal sheet, and the bending radius of the U-shaped cutting sleeve is the same as the radius of the operating bracket.
In a preferred embodiment, the drive shaft extends outside the cassette body and the outer end is fixedly provided with a turning handle.
In a preferred embodiment, the two rotating wheels are arranged in the same shape, the movable positioning rod is arranged in a circular arc shape, the axis of the circular arc is consistent with the rotating shaft, and the elastic coefficients of the two return springs are arranged in the same manner.
A use method of a kit for blood sample pretreatment is characterized by comprising the following steps: the method specifically comprises the following operation steps:
the method comprises the following steps: opening a box cover on a box main body, firstly putting the collected blood into a sample test tube, then enabling the sample test tube to pass through a test tube placing hole on a test tube placing plate, and placing the bottom end of the sample test tube into a groove on a test tube supporting plate to realize the placement of the sample test tube;
step two: opening a test tube plug on the sample test tube, dropwise adding an acetonitrile solvent into the placed sample test tube, wherein the volume ratio of the blood plasma to the acetonitrile solvent is more than or equal to 1: 3, and then plugging the test tube plug back onto the sample test tube again;
step three: operating a test tube placing plate with a sample test tube dropwise added with an acetonitrile solvent reagent, pressing down a lifting clamping column in a movable hole, inserting the lifting clamping column into a connecting clamping hole in a rotating shaft, and extruding the lifting clamping column by a rubber ring by utilizing the elasticity of the rubber ring to prevent the lifting clamping column from rising back;
step four: operating a test tube placing plate of a sample test tube which is not dripped with an acetonitrile solvent reagent, pulling up a lifting clamping column in a movable hole, fastening a U-shaped clamping sleeve at the top end of the lifting clamping column with an operating bracket, fixing the lifting clamping column, and preventing the lifting clamping column from being inserted into a connecting clamping hole on a rotating shaft;
step five: the rotation handle on the operation drive shaft, make the drive shaft drive the rotation wheel and rotate, the rotation wheel on the drive shaft utilizes the latch meshing to carry out the transmission with the rotation wheel on the axis of rotation, it rotates to drive the axis of rotation, the axis of rotation utilizes the rotation support to drive the movable cylinder and moves on the movable positioning rod, compress the reset spring of direction of motion one side, when two rotation wheel latches are not meshing, the reset spring of compression on the movable positioning rod promotes the restoration of movable cylinder, the reset spring of cooperation opposite side, make the movable cylinder restore to the initial position, and then realize the restoration of axis of rotation, thereby it inserts the test tube placing plate of connecting the card hole on the axis of rotation to drive the lift kelly and swings repeatedly, sample test tube on this test tube placing plate oscillates;
step six: the impurity protein in the plasma in the sample test tube after dropping the acetonitrile solvent reagent and oscillating is separated to form floccule, and the catenin is attached to the floccule, thereby realizing the pretreatment of the blood sample without influencing other samples in the main body of the box.
The invention has the technical effects and advantages that:
1. according to the test tube placing plate, the lifting clamping columns and the connecting clamping holes are arranged, the test tube placing plate is in selective transmission connection with the rotating shaft by means of plug-in clamping of the connecting clamping columns and the connecting clamping holes, so that sample test tubes placed on the test tube placing plate can be conveniently controlled to oscillate, the sample test tubes on other test tube placing plates are not affected, and compared with an existing kit, the test tube placing plate has the advantages that specific sample test tubes can be conveniently preprocessed, manual oscillation operation of the sample test tubes one by one is avoided, and test efficiency is improved;
2. according to the invention, the rotating shaft is arranged, the rotating shaft and the driving shaft are utilized, the rotating wheels with the clamping teeth are rotatably connected, the driving shaft drives the rotating shaft to rotate, the movable barrel at the bottom end of the rotating support moves on the movable positioning rod, and the position restoration of the rotating shaft is realized under the condition that the clamping teeth of the two rotating wheels are not meshed by matching with the return springs at the two ends of the movable barrel, so that the rotating shaft drives the test tube to be placed and rotated back and forth, and the oscillation operation of the sample test tube is realized.
Drawings
Fig. 1 is an overall schematic view of the present invention.
FIG. 2 is a side cross-sectional view of the handling frame of the present invention.
FIG. 3 is a schematic view of portion A of FIG. 2 according to the present invention.
Fig. 4 is an overall schematic view of the handling stand according to the present invention.
FIG. 5 is a schematic top view of the cassette body of the present invention.
FIG. 6 is a schematic view of the portion B of FIG. 5 according to the present invention.
FIG. 7 is a schematic side sectional view of a rotatable wheel of the present invention.
The reference signs are: 1 box main part, 2 axis of rotation, 3 the test tube place the board, 4 turning blocks, 5 operation support, 6 test tube layer boards, 7 test tubes place the hole, 8 recesses, 9 sample test tubes, 10 movable holes, 11 rubber rings, 12 lift ka zhu, 13 connect the card hole, 14U-shaped cutting ferrule, 15 drive shafts, 16 rotation wheels, 17 activity locating levers, 18 activity section of thick bamboo, 19 rotation support, 20 reset spring, 21 lid, 22 test tubes compress tightly the cushion, 23 shock attenuation rubber rings, 24 rotation handles.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a kit for pretreating a blood sample as shown in figures 1-6, which comprises a box main body 1, wherein a rotating shaft 2 is movably arranged in the box main body 1, a test tube placing plate 3 is arranged at the top of the rotating shaft 2, rotating blocks 4 are fixedly arranged on two sides of the test tube placing plate 3, and the rotating blocks 4 are movably connected with the rotating shaft 2;
an operation support 5 is fixedly arranged on the surface of the test tube placing plate 3, the operation support 5 is arranged in a U shape, two ends of the operation support are vertically arranged downwards, a test tube supporting plate 6 is fixedly arranged at the bottom of the operation support 5, test tube placing holes 7 are formed in two ends of the surface of the test tube placing plate 3, grooves 8 are formed in two ends of the surface of the test tube supporting plate 6, the test tube placing holes 7 and the grooves 8 are arranged in a vertical collinear mode, and sample test tubes 9 are placed between the test tube placing holes 7 and the grooves 8;
the test tube placing plate 3 middle part is fixed and has been seted up the activity hole 10, the fixed cover in activity hole 10 is equipped with rubber circle 11, the inside activity of rubber circle 11 is provided with lift card post 12, 2 fixed surface on the axis of rotation have seted up connector hole 13, lift card post 12 and connector hole 13 activity joint, the fixed U-shaped cutting ferrule 14 that is provided with in lift card post 12 top, U-shaped cutting ferrule 14 and 5 lock joints of operation support.
Further, 1 cross sectional shape of box main part sets up to trapezoidal, and the top sets up for the opening, 1 surperficial one side of box main part is articulated to be provided with lid 21, the fixed test tube that is provided with in the inside both sides of lid 21 compresses tightly cushion 22, the test tube compresses tightly cushion 22 and places board 3 top phase-matches with the test tube, and when convenient box main part 1 is sealed to lid 21, it is fixed to utilize the test tube to compress tightly cushion 22 and place the sample test tube 9 on board 3 to the test tube, avoids the vibration in the kit transportation to cause the influence to the sample test tube.
Further, the number of the test tube placing plates 3 is seven, and the seven test tube placing plates 3 are sequentially arranged at the top of the rotating shaft 2 and are arranged in a cross manner with the rotating shaft 2, so that a plurality of sample test tubes can be conveniently placed.
Further, operation support 5 is made by stainless steel material, and operation support 5 sets up that the part that the board 3 bottoms were placed to the test tube is greater than the part that sets up in the board 3 tops is placed to the test tube, the test tube is placed the inside fixed cover in hole 7 and is equipped with shock attenuation rubber ring 23, conveniently utilizes the gravity of operation support 5 self, realizes that the test tube places the level state of board 3 in axis of rotation 2, and cooperation shock attenuation rubber ring 23 makes things convenient for sample test tube 9 to place in hole 7 is placed to the test tube.
Further, lift ka zhu 12 is made by metal material, the operation support 5 is same vertical plane setting with lift ka zhu 12, U-shaped cutting ferrule 14 is made by elastic metal piece, U-shaped cutting ferrule 14 bending radius sets up the same with operation support 5 radius, improves lift ka zhu's 12 intensity, makes things convenient for the test tube to place the fixed connection of board 3 and axis of rotation 2, and the convenience is when lift ka zhu 12 rises the operation, utilizes U-shaped cutting ferrule 14 and 5 tops of operation support to carry out the joint, fixes the lift ka zhu 12 of the state that rises.
The implementation mode is specifically as follows: operating the test tube placing plate 3 with the sample test tube 9 dripped with acetonitrile solvent reagent, pressing down the lifting clamp column 12 in the movable hole 10, inserting the lifting clamp column 12 into the connecting clamp hole 13 on the rotating shaft 2, extruding the lifting clamp column 12 by the rubber ring 11 by utilizing the elasticity of the rubber ring to prevent the lifting clamp column 12 from rising back, operating the test tube placing plate 3 with the sample test tube 9 not dripped with acetonitrile solvent reagent, pulling up the lifting clamp column 12 in the movable hole 10, fastening the U-shaped clamp sleeve 14 at the top end of the lifting clamp column 12 with the operating bracket 5, fixing the lifting clamp column 12, preventing the lifting clamp column 12 from being inserted into the connecting clamp hole 13 on the rotating shaft 2, realizing the selective transmission connection of the test tube placing plate 3 and the rotating shaft 2, further controlling the sample test tube 9 placed on the test tube placing plate 3 to oscillate, not influencing the sample test tubes 9 on other test tube placing plates 3, and facilitating the pretreatment of the specific sample test tube 9, the sample test tubes are prevented from being manually oscillated one by one, so that the test efficiency is improved;
as shown in fig. 1 and 7, a reagent kit for blood sample pretreatment, a driving shaft 15 is movably arranged at one end of a main body 1 of the reagent kit, the driving shaft 15 and the rotating shaft 2 are vertically arranged in a collinear way, one end of the rotating shaft 2 and one end of the driving shaft 15 are both fixedly provided with rotating wheels 16, one third of the surfaces of the two rotating wheels 16 are both fixedly provided with clamping teeth, the two rotating wheels 16 are meshed through the clamping teeth, a movable positioning rod 17 is fixedly arranged on one side inside the box main body 1, a movable cylinder 18 is sleeved on the surface of the movable positioning rod 17, one side of the rotating wheel 16 is provided with a rotating bracket 19, the top end of the rotating bracket 19 is fixedly connected with the rotating shaft 2, the bottom end of the rotating support 19 is fixedly connected with the movable barrel 18, two ends of the movable barrel 18 are respectively provided with a return spring 20, and the return springs 20 are sleeved with the movable positioning rods 17.
The driving shaft 15 extends out of the box main body 1, and a rotating handle 24 is fixedly arranged at the outer end part of the driving shaft 15, so that the driving shaft 15 can be operated conveniently to rotate.
Furthermore, the two rotating wheels 16 are identical in shape, the movable positioning rod 17 is arc-shaped, the axis of the arc is identical to the axis of rotation 2, the elastic coefficient of the two return springs 20 is identical, the driving shaft 15 and the axis of rotation 2 are in transmission connection by means of the latch on the two rotating wheels 16, the return springs 20 are convenient to drive the movable cylinder 18, and the rotary support 19 is matched to drive the position of the axis of rotation 2 to be restored.
The implementation mode is specifically as follows: when the oscillation operation of the sample test tube 9 is performed, the rotating handle 24 on the driving shaft 15 is operated to make the driving shaft 15 drive the rotating wheel 16 to rotate, the rotating wheel 16 on the driving shaft 15 and the rotating wheel 16 on the rotating shaft 2 are engaged to perform transmission by using the latch, the rotating shaft 2 drives the rotating cylinder 18 to move on the movable positioning rod 17 by using the rotating bracket 19, the return spring 20 on one side of the moving direction is compressed, when the latches of the two rotating wheels 16 are not engaged, the compressed return spring 20 on the movable positioning rod 17 pushes the moving cylinder 18 to restore, and the return spring 20 on the other side is matched to restore the moving cylinder 18 to the initial position, so as to realize the restoration of the rotating shaft 2, and no matter the driving shaft 15 is rotated clockwise or counterclockwise, one of the two return springs 20 is compressed, thereby driving the lifting clamping column 12 to be inserted into the test tube placing plate 3 connected with the clamping hole 13 on the rotating shaft 2 to perform the repeated oscillation, the sample tubes 9 on the tube placing plate 3 are oscillated.
A use method of a kit for blood sample pretreatment is characterized by comprising the following steps: the method specifically comprises the following operation steps:
the method comprises the following steps: opening a box cover 21 on a box main body 1, firstly putting the collected blood into a sample test tube 9, then enabling the sample test tube 9 to pass through a test tube placing hole 7 on a test tube placing plate 3, and placing the bottom end of the sample test tube 9 into a groove 8 on a test tube supporting plate 6 to realize the placement of the sample test tube 9;
step two: opening a test tube plug on the sample test tube 9, dropwise adding acetonitrile solvent into the placed sample test tube 9, wherein the volume ratio of the plasma to the acetonitrile solvent is more than or equal to 1: 3, and then plugging the test tube plug back onto the sample test tube 9 again;
step three: operating the test tube placing plate 3 with the sample test tube 9 dropwise added with the acetonitrile solvent reagent, pressing down the lifting clamping column 12 in the movable hole 10, inserting the lifting clamping column 12 into the connecting clamping hole 13 on the rotating shaft 2, and extruding the lifting clamping column 12 by the rubber ring 11 by utilizing the elasticity of the rubber ring to prevent the lifting clamping column 12 from rising back;
step four: operating the test tube placing plate 3 of the sample test tube 9 which is not dripped with the acetonitrile solvent reagent, pulling up the lifting clamping column 12 in the movable hole 10, fastening the U-shaped clamping sleeve 14 at the top end of the lifting clamping column 12 with the operating bracket 5, fixing the lifting clamping column 12, and preventing the lifting clamping column 12 from being inserted into the connecting clamping hole 13 on the rotating shaft 2;
step five: operating a rotating handle 24 on a driving shaft 15 to enable the driving shaft 15 to drive a rotating wheel 16 to rotate, wherein the rotating wheel 16 on the driving shaft 15 and the rotating wheel 16 on the rotating shaft 2 are meshed by using a latch to drive the rotating shaft 2 to rotate, the rotating shaft 2 drives a movable cylinder 18 to move on a movable positioning rod 17 by using a rotating support 19, and a return spring 20 on one side of the moving direction is compressed, when the two rotating wheels 16 are not meshed, the compressed return spring 20 on the movable positioning rod 17 pushes the movable cylinder 18 to restore, and the movable cylinder 18 is restored to an initial position by matching with the return spring 20 on the other side, so that the restoring of the rotating shaft 2 is realized, and a lifting clamping column 12 is driven to be inserted into a test tube placing plate 3 connected with a clamping hole 13 on the rotating shaft 2 to repeatedly swing to oscillate a sample test tube 9 on the test tube placing plate 3;
step six: the impurity protein in the plasma in the sample test tube 9 after dropping the acetonitrile solvent reagent and oscillating is separated to form floccule, and the catenin is attached to the floccule, thereby realizing the pretreatment of the blood sample without influencing other samples in the main body 1 of the box.
The points to be finally explained are: first, in the description of the present application, it should be noted that, unless otherwise specified and limited, the terms "mounted," "connected," and "connected" should be understood broadly, and may be a mechanical connection or an electrical connection, or a communication between two elements, and may be a direct connection, and "upper," "lower," "left," and "right" are only used to indicate a relative positional relationship, and when the absolute position of the object to be described is changed, the relative positional relationship may be changed;
secondly, the method comprises the following steps: in the drawings of the disclosed embodiments of the invention, only the structures related to the disclosed embodiments are referred to, other structures can refer to common designs, and the same embodiment and different embodiments of the invention can be combined with each other without conflict;
and finally: the above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that are within the spirit and principle of the present invention are intended to be included in the scope of the present invention.
Claims (7)
1. The utility model provides a kit for blood sample preliminary treatment, includes box main part (1), its characterized in that: the box comprises a box main body (1) and is characterized in that a rotating shaft (2) is movably arranged in the box main body (1), a test tube placing plate (3) is arranged at the top of the rotating shaft (2), rotating blocks (4) are fixedly arranged on two sides of the test tube placing plate (3), the rotating blocks (4) are movably connected with the rotating shaft (2), the number of the test tube placing plates (3) is seven, the seven test tube placing plates (3) are sequentially arranged at the top of the rotating shaft (2) and are arranged in a cross mode with the rotating shaft (2);
an operation support (5) is fixedly arranged on the surface of the test tube placing plate (3), the operation support (5) is in a U shape, two ends of the operation support are vertically arranged downwards, a test tube supporting plate (6) is fixedly arranged at the bottom of the operation support (5), test tube placing holes (7) are formed in two ends of the surface of the test tube placing plate (3), grooves (8) are formed in two ends of the surface of the test tube supporting plate (6), the test tube placing holes (7) and the grooves (8) are vertically arranged in a collinear manner, and sample test tubes (9) are placed between the test tube placing holes (7) and the grooves (8);
a movable hole (10) is fixedly formed in the middle of the test tube placing rack (3), a rubber ring (11) is fixedly sleeved in the movable hole (10), a lifting clamping column (12) is movably arranged in the rubber ring (11), a connecting clamping hole (13) is fixedly formed in the surface of the rotating shaft (2), the lifting clamping column (12) is movably clamped with the connecting clamping hole (13), a U-shaped clamping sleeve (14) is fixedly arranged at the top end of the lifting clamping column (12), and the U-shaped clamping sleeve (14) is fastened with the operating bracket (5);
the box is characterized in that a driving shaft (15) is movably arranged at one end of the box main body (1), the driving shaft (15) and the rotating shaft (2) are vertically arranged in a collinear manner, a rotating wheel (16) is fixedly arranged at one end of the rotating shaft (2) and one end of the driving shaft (15), two rotating wheels (16) are fixedly arranged at one third of the surface of the rotating wheel (16) and provided with a latch, the rotating wheels (16) are arranged through the latch in a meshed manner, a movable positioning rod (17) is fixedly arranged at one side inside the box main body (1), a movable cylinder (18) is sleeved on the surface of the movable positioning rod (17), a rotating support (19) is arranged at one side of the rotating wheel (16), the top end of the rotating support (19) is fixedly connected with the rotating shaft (2), the bottom end of the rotating support (19) is fixedly connected with the movable cylinder (18), the return spring (20) is sleeved with the movable positioning rod (17).
2. The kit for blood sample pretreatment according to claim 1, wherein: box main part (1) cross sectional shape sets up to trapezoidal, and the top sets up for the opening, box main part (1) surface one side is articulated to be provided with lid (21), the fixed test tube that is provided with in lid (21) inside both sides compresses tightly cushion (22), the test tube compresses tightly cushion (22) and places board (3) top phase-match with the test tube.
3. The kit for blood sample pretreatment according to claim 1, wherein: the operation support (5) are made by stainless steel material, and the operation support (5) set up in the test tube place the part of board (3) bottom be greater than set up in the test tube place the part at board (3) top, the test tube is placed the fixed cover in hole (7) and is equipped with shock attenuation rubber ring (23).
4. The kit for blood sample pretreatment according to claim 1, wherein: the lifting clamping column (12) is made of a metal material, the operating support (5) and the lifting clamping column (12) are arranged on the same vertical plane, the U-shaped clamping sleeve (14) is made of an elastic metal sheet, and the bending radius of the U-shaped clamping sleeve (14) is the same as that of the operating support (5).
5. The kit for blood sample pretreatment according to claim 1, wherein: the driving shaft (15) extends out of the box main body (1), and a rotating handle (24) is fixedly arranged at the outer end part of the driving shaft.
6. The kit for blood sample pretreatment according to claim 1, wherein: the two rotating wheels (16) are arranged in the same shape, the movable positioning rod (17) is arranged in a circular arc shape, the axis of the circular arc is consistent with that of the rotating shaft (2), and the elastic coefficients of the two return springs (20) are arranged in the same manner.
7. A use method of a kit for blood sample pretreatment is characterized by comprising the following steps: the method specifically comprises the following operation steps:
the method comprises the following steps: opening a box cover (21) on a box main body (1), firstly putting collected blood into a sample test tube (9), then enabling the sample test tube (9) to pass through a test tube placing hole (7) on a test tube placing plate (3), and placing the bottom end of the sample test tube (9) into a groove (8) on a test tube supporting plate (6) to realize the placement of the sample test tube (9);
step two: opening a test tube plug on the sample test tube (9), dripping acetonitrile solvent into the placed sample test tube (9), wherein the volume ratio of the blood plasma to the acetonitrile solvent is more than or equal to 1: 3, and then plugging the test tube plug back onto the sample test tube (9);
step three: operating a test tube placing plate (3) of a sample test tube (9) dropwise added with an acetonitrile solvent reagent, pressing down a lifting clamping column (12) in a movable hole (10), inserting the lifting clamping column (12) into a connecting clamping hole (13) on a rotating shaft (2), and extruding the lifting clamping column (12) by a rubber ring (11) by utilizing the elasticity of the rubber ring (11) to prevent the lifting clamping column (12) from rising back;
step four: operating a test tube placing plate (3) of a sample test tube (9) which is not dripped with an acetonitrile solvent reagent, pulling up a lifting clamping column (12) in a movable hole (10), fastening a U-shaped clamping sleeve (14) at the top end of the lifting clamping column (12) with an operating bracket (5), fixing the lifting clamping column (12), and preventing the lifting clamping column (12) from being inserted into a connecting clamping hole (13) on a rotating shaft (2);
step five: operating a rotating handle (24) on a driving shaft (15), enabling the driving shaft (15) to drive a rotating wheel (16) to rotate, enabling the rotating wheel (16) on the driving shaft (15) and the rotating wheel (16) on a rotating shaft (2) to be meshed by using a latch for transmission, driving the rotating shaft (2) to rotate, enabling the rotating shaft (2) to drive a movable cylinder (18) to move on a movable positioning rod (17) by using a rotating support (19), compressing a return spring (20) at one side of the moving direction, enabling the compressed return spring (20) on the movable positioning rod (17) to push the movable cylinder (18) to restore when the latches of the two rotating wheels (16) are not meshed, matching with the return spring (20) at the other side, enabling the movable cylinder (18) to restore to the initial position, further realizing the restoration of the rotating shaft (2), and driving a lifting clamping column (12) to be inserted into a test tube placing plate (3) connected with a clamping hole (13) on the rotating shaft (2, oscillating the sample tubes (9) on the tube holding plate (3);
step six: the impurity protein in the plasma in the sample test tube (9) dripped with the acetonitrile solvent reagent and oscillated is separated to form floccule, and the desmosine is attached to the floccule, thereby realizing the pretreatment of the blood sample without influencing other samples in the box main body (1).
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| CN201910912117.XA CN110639638B (en) | 2019-09-25 | 2019-09-25 | Kit for pretreating blood sample and using method thereof |
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| CN103149349B (en) * | 2013-03-07 | 2014-04-09 | 深圳市理邦精密仪器股份有限公司 | Automatic medical tested object pop-up structure and blood-gas analyzer employing same |
| CN104386629B (en) * | 2014-09-22 | 2016-06-29 | 深圳市奥特库贝科技有限公司 | A kind of novel agent bottle automatically opening and closing mechanism |
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| EP0736481A2 (en) * | 1995-04-06 | 1996-10-09 | Roche Diagnostics GmbH | Device and method for withdrawing or unscrewing closures from containers and gripping mandrell for closures |
| CN204193982U (en) * | 2014-08-27 | 2015-03-11 | 珠海倍健电子科技有限公司 | Band mixes separately the rack for test tube device of function |
| CN107029609A (en) * | 2017-05-19 | 2017-08-11 | 金柯楠 | A kind of medical drug is prepared and uses mechanical oscillation device |
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