CN110632225A - On-line solid phase extraction liquid chromatography for detecting josamycin content in blood - Google Patents
On-line solid phase extraction liquid chromatography for detecting josamycin content in blood Download PDFInfo
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- CN110632225A CN110632225A CN201910711062.6A CN201910711062A CN110632225A CN 110632225 A CN110632225 A CN 110632225A CN 201910711062 A CN201910711062 A CN 201910711062A CN 110632225 A CN110632225 A CN 110632225A
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- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G01N2030/8822—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving blood
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Abstract
The invention belongs to the field of medical inspection, relates to a method for measuring the content of an in-vivo antibacterial drug, and particularly relates to an online solid-phase extraction liquid chromatography for detecting the content of josamycin in blood. The invention relates to an on-line solid phase extraction liquid chromatography for detecting the content of josamycin in blood, which is characterized in that after a blood sample to be detected is pretreated, a high performance liquid chromatograph is used for determining the content of josamycin by adopting a standard curve method.
Description
Technical Field
The invention belongs to the field of medical inspection, relates to a method for measuring the content of an in-vivo antibacterial drug, and particularly relates to an online solid-phase extraction liquid chromatography for detecting the content of josamycin in blood.
Background
Josamycin is a single-component 14-carbon macrolide antibiotic, and the pharmacological characteristics and clinical application of josamycin are similar to those of midecamycin and spiramycin. Mainly combines with bacterial 50S ribosome subunit, and inhibits the synthesis of bacterial protein by blocking transpeptidation and mRNA translocation, thereby playing the role of antibacterium. The antibacterial performance is similar to that of erythromycin. Has antibacterial effect against Staphylococcus, Streptococcus, Clostridium, diphtheria, Bacillus anthracis, Neisseria, Legionella, Brucella, Campylobacter, Mycoplasma, Chlamydia, and Rickettsia. The josamycin has good antibacterial effect on meningococcus, bordetella pertussis, peptococcus, streptococcus digestions, propionibacterium, eubacterium and the like. When the blood concentration of josamycin is more than 5.0mg/L, the toxic and side effect is enhanced. Can cause gastrointestinal tract reaction: nausea, vomiting, loss of appetite, abdominal distension, diarrhea. Because the VCMC has a narrow therapeutic window and large individual difference in the metabolic process in vivo, the monitoring of the blood concentration is an important reference for adjusting the dosage and reducing adverse reactions, and has great significance in clinical medication.
At present, a plurality of methods for measuring the blood concentration of josamycin are commonly used, but the problems of complex pretreatment, high cost, high requirements on experimenters and environment, long analysis time and the like still exist at present.
Disclosure of Invention
The invention aims to provide an online solid-phase extraction liquid chromatography for detecting the content of josamycin in blood aiming at the current situation of the prior art.
The technical scheme adopted by the invention for solving the technical problem is as follows:
an on-line solid phase extraction liquid chromatography for detecting josamycin content in blood is characterized by comprising the following steps:
(1) pretreatment of plasma samples: taking a sample to be detected, and adding a protein precipitator for precipitation;
(2) drawing a standard curve, namely taking 20uL standard working solution, adding blank plasma to 1ml, uniformly mixing to obtain standard solutions with 6 concentrations, respectively adding a protein precipitator into the obtained standard solutions for precipitation, centrifuging at 9000-15000rpm for 10-15min, respectively taking supernate, detecting the supernate by using a high performance liquid chromatograph and a DAD detector to obtain a josamycin spectrogram in the standard solutions, taking the peak area of the josamycin as the ordinate y of a standard curve graph, taking the concentration of the josamycin contained in the standard working solution as the abscissa x of the standard curve graph, performing linear regression on data, fitting to obtain a standard curve equation of y ═ x + b, and obtaining weight coefficients a, b and a correlation coefficient R2(ii) a The standard curve is drawn for at least three times by using three different batches of standard working solution; the standard working solution is a solution containing josamycin;
(3) centrifuging the detected blood, namely centrifuging at least 2ml of the detected blood at the centrifugation speed of 3500-4500rpm for 10-15min to obtain supernatant, and storing the supernatant at-20 ℃ for later use;
(4) and (3) detection of a sample to be detected:
s1, heating the mixture in a water bath (37 ℃), and re-melting the supernatant obtained in the step (3);
s2, using a liquid transfer gun to transfer the supernatant 800-1000uL after the re-melting in the step S1, and performing pretreatment according to the step (1) and centrifuging to obtain the supernatant which is the sample to be detected;
and S3, transferring the sample to be detected 600-800uL in the step (c), detecting the sample to be detected by using a high performance liquid chromatograph and a DAD detector to obtain a josamycin chromatogram of the sample to be detected, substituting the josamycin peak area y in the chromatogram into the standard curve equation y ═ a x + b in the step (2), and calculating to obtain the concentration of the josamycin in the sample to be detected.
The protein precipitant is acetonitrile.
Further, the standard working solution in the step (2) is a josamycin solution with the concentration of 1.0, 2.5, 4.0, 5.5, 7.5 and 9.0 mu g/mL.
Further, the high performance liquid chromatograph in the step (2) comprises a sample injection module, a liquid phase pump module, a column temperature module, a detector module, an extraction pump, a liquid supplement pump, a six-way valve, an analysis column and an SPE column.
Furthermore, the mobile phase of the SPE column is 0.02-0.04% phosphoric acid water solution, the flow rate is 0.4-0.5mL/min, and the SPE column adopts a backflushing mode.
Further, the mobile phase of the analytical column is a 20% organic phase and an 80% aqueous phase, the organic phase is acetonitrile, and the aqueous phase is a 0.02-0.04% phosphoric acid aqueous solution; the flow rate is 1-1.2mL/min, and the analytical column adopts a gradient elution mode.
Further, the detection wavelength of the DAD detector in the step (2) is 242 nm.
The invention has the advantages that: according to the on-line solid-phase extraction liquid phase analysis method for detecting the concentration of the josamycin in blood, plasma is subjected to protein precipitation and then directly subjected to sample injection, so that the operation of personnel is reduced, the accuracy of a quantitative result is improved, the analysis time is greatly shortened, the detection process is simple, convenient and quick, the experiment cost is reduced, the monitoring of the blood concentration of the josamycin in a patient body in clinical treatment is facilitated, and the experiment basis is provided for personalized administration of the josamycin and reduction of toxic and side reactions.
Drawings
FIG. 1 is a schematic diagram of the working flow of on-line solid phase extraction analysis.
FIG. 2 is a chromatogram of josamycin in a standard solution according to the present invention;
FIG. 3 is a chromatogram of josamycin in a spiked plasma sample according to the present invention.
Detailed Description
In order to make the technical means, the original characteristics, the achieved purposes and the effects of the invention easy to understand, the invention is further described with reference to the figures and the specific embodiments.
Example 1
Pretreatment of plasma samples: taking a sample to be detected, and adding a protein precipitator for precipitation.
Drawing of (II) standard curve
Firstly, placing 20uL of standard working solution into a 10mL centrifuge tube by using a pipette, adding blank plasma into 1mL, uniformly mixing to prepare standard solutions with 6 concentrations, respectively adding a protein precipitator into the standard solutions to precipitate, centrifuging for 10-15min by using a centrifuge with the rotation speed of 15000rpm, respectively taking 500uL of the supernatant, detecting the supernatant by using a high performance liquid chromatograph and a DAD detector to obtain a josamycin spectrogram in the standard solutions, taking the peak area of the josamycin as the ordinate y of a standard curve graph, taking the concentration of the josamycin contained in the standard working solution as the abscissa x of the standard curve graph, performing linear regression on data, fitting to obtain a standard curve equation of y a x + b, and obtaining weight coefficients a, b and a correlation coefficient R2. The standard curve is plotted at least three times with three different batches of standard working fluid. The standard working solution is a solution containing josamycin.
Preparing a standard working solution:
(a) accurately weighing 10mg of josamycin standard substance, placing the josamycin standard substance into a 1mL volumetric flask, dissolving the josamycin standard substance with methanol and fixing the volume to obtain a standard stock solution A, wherein the concentration of the josamycin is 10mg/mL, diluting the standard stock solution A with blood plasma, preparing standard working solutions with various concentrations in the range containing 15-600 mu g/mL of josamycin respectively, and storing the standard working solutions at-80 ℃;
(III) centrifugation of test blood
Centrifuging at least 2ml of blood to be detected at a centrifugal speed of 3500rpm for 10min to obtain supernatant, and freezing the supernatant at-20 deg.C for storage until the supernatant is ready for analysis;
(IV) treatment of the sample to be tested
(b) Heating with water bath (37 deg.C), and re-melting the frozen plasma of step (III).
(c) And (c) transferring 1000uL of the plasma under the item (b) into a 10mL centrifuge tube by using a pipette gun, pretreating according to the step (I), and centrifuging to obtain a supernatant, namely the sample to be detected.
(IV) detection of sample to be tested
And (c) transferring 800uL of the sample to be detected in the step (c), detecting the sample to be detected by using a high performance liquid chromatograph and a DAD detector to obtain a josamycin chromatogram of the sample to be detected, substituting the peak area y of the josamycin in the chromatogram into the standard curve equation y ═ a x + b in the step (II), and calculating to obtain the concentration of the josamycin in the sample to be detected.
In the step (II), six standard working solutions with different concentrations are used, and the six standard working solutions with different concentrations are josamycin solutions with concentrations of 1.0, 2.5, 4.0, 5.5, 7.5 and 9.0 mu g/mL respectively;
as shown in fig. 1, the conventional high performance liquid chromatograph includes: advance kind module, liquid phase pump module, column temperature module and detector module, high performance liquid chromatograph still includes: the device comprises an extraction pump, a liquid supplementing pump, a six-way valve and an SPE column, wherein the SPE column is SPENJH 301002; the SPE column mobile phase of the on-line solid phase extractor is 0.02 percent phosphoric acid water solution, and the flow rate is 0.4 mL/min; the mobile phase of the fluid infusion pump is pure water, and the flow rate is 1.0 mL/min; the SPE column uses a backflush mode.
Analytical column mobile phase contains 20:80 ratio of organic phase: an aqueous phase, wherein the organic phase is acetonitrile, the aqueous phase is an aqueous solution containing 0.02% phosphoric acid, the flow rate of an analytical chromatographic column is 1.0mL/min, and the analytical chromatographic column adopts an isocratic elution mode;
the DAD detector is Thermo DAD-3000, and the detection wavelength of the DAD detector is 242 nm.
The technical method in this example is demonstrated as follows:
first, the linear relationship and quantitative limits of the method
Adding 980 mul of blank plasma into the prepared 20 mul of josamycin standard working solution with each concentration, uniformly mixing, diluting, and then injecting a sample, wherein the concentration of the josamycin is in the range of 0.3mg/L to 12mg/L, according to the determination conditions of the embodiment, determining according to the concentration from low to high, and drawing a quantitative chromatographic peak area-concentration graph to obtain a standard curve, and the result shows that the linear range and the quantitative limit of the josamycin are as follows:
(1) limit of detection (LOD): 0.0.5 mg/L.
(2) Limit of quantitation (LOQ): 0.3 mg/L.
(3) Linear range:
the josamycin is in the range of 0.3mg/L to 18mg/L, the linearity is good, and the correlation coefficient R2＞0.99。
Second, the recovery rate and precision of the method
The josamycin standard working solution is prepared into high, medium and low concentrations of 3 for sample adding recovery rate experiments and precision experiments, the determination is carried out according to the method of the embodiment, and the analysis and the determination are repeated for 3 batches, wherein the recovery rate and the precision are respectively shown in the table 1. The average recovery rate of the josamycin is 93.1-103.2% in the range of 3 addition levels of low, medium and high, the relative standard deviation is 1.17-1.64%, and the table 1 shows the standard recovery rate and precision of josamycin.
| Addition amount/(mg/L) | 2.0 | 5.0 | 8.0 |
| Average recovery rate/%) | 93.1 | 103.2 | 96.5 |
| Precision RSD/%) | 1.17 | 1.64 | 1.42 |
TABLE 1 recovery and precision of josamycin spiking
Thirdly, stability of the solution
The prepared precision sample of josamycin was used to determine the stability of the sample solution according to the method of this example. The results of the experiment show that the sample is stable within 7 hours.
By integrating the verification tests, the detection limit, the recovery rate, the precision, the stability and other technical indexes of the embodiment meet the requirements, and the method for detecting the concentration of the josamycin medicine in blood has the advantages of good reproducibility, high sample recovery rate and improvement on the accuracy of the detection result.
The chromatogram of the josamycin in the standard solution is shown in figure 2, the chromatogram of the josamycin in the serum sample is shown in figure 3, the retention time of the josamycin is 8.15min, and as can be seen from figures 2 and 3, the method has the advantages of accurate identification of the target compound, short analysis time, small interference and strong specificity.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are given by way of illustration of the principles of the present invention, and that various changes and modifications may be made without departing from the spirit and scope of the invention as defined by the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (7)
1. An on-line solid phase extraction liquid chromatography for detecting josamycin content in blood is characterized by comprising the following steps:
(1) pretreatment of plasma samples: taking a sample to be detected, and adding a protein precipitator for precipitation;
(2) drawing a standard curve, namely taking 20uL standard working solution, adding blank plasma to 1ml, uniformly mixing to obtain standard solutions with 6 concentrations, respectively adding a protein precipitator into the obtained standard solutions for precipitation, centrifuging at 9000-15000rpm for 10-15min, respectively taking supernate, detecting the supernate by using a high performance liquid chromatograph and a DAD detector to obtain a josamycin spectrogram in the standard solutions, taking the peak area of the josamycin as the ordinate y of a standard curve graph, taking the concentration of the josamycin contained in the standard working solution as the abscissa x of the standard curve graph, performing linear regression on data, fitting to obtain a standard curve equation of y ═ x + b, and obtaining weight coefficients a, b and a correlation coefficient R2(ii) a The standard curve is drawn for at least three times by using three different batches of standard working solution; the standard working solution is a solution containing josamycin;
(3) centrifuging the detected blood, namely centrifuging at least 2ml of the detected blood at the centrifugation speed of 3500-4500rpm for 10-15min to obtain supernatant, and storing the supernatant at-20 ℃ for later use;
(4) and (3) detection of a sample to be detected:
s1, heating the mixture in a water bath (37 ℃), and re-melting the supernatant obtained in the step (3);
s2, using a liquid transfer gun to transfer the supernatant 800-1000uL after the re-melting in the step S1, and performing pretreatment according to the step (1) and centrifuging to obtain the supernatant which is the sample to be detected;
and S3, transferring the sample to be detected 600-800uL in the step (c), detecting the sample to be detected by using a high performance liquid chromatograph and a DAD detector to obtain a josamycin chromatogram of the sample to be detected, substituting the josamycin peak area y in the chromatogram into the standard curve equation y ═ a x + b in the step (2), and calculating to obtain the concentration of the josamycin in the sample to be detected.
2. The liquid chromatography method of claim 1, wherein: the protein precipitant is acetonitrile.
3. The liquid chromatography method of claim 1, wherein: the standard working solution in the step (2) is a josamycin solution with the concentration of 1.0, 2.5, 4.0, 5.5, 7.5 and 9.0 mu g/mL.
4. The liquid chromatography method of claim 1, wherein: the high performance liquid chromatograph in the step (2) comprises a sample injection module, a liquid phase pump module, a column temperature module and a detector module, and further comprises an extraction pump, a liquid supplement pump, a six-way valve, an analysis column and an SPE column.
5. A liquid chromatography method according to claim 4, characterized in that: the mobile phase of the SPE column is 0.02-0.04% phosphoric acid water solution, the flow rate is 0.4-0.5mL/min, and the SPE column adopts a backflushing mode.
6. The liquid chromatography method of claim 4, wherein: the mobile phase of the analytical column is a 20% organic phase and an 80% aqueous phase, the organic phase is acetonitrile, and the aqueous phase is a 0.02-0.04% phosphoric acid aqueous solution; the flow rate is 1-1.2mL/min, and the analytical column adopts a gradient elution mode.
7. A liquid chromatography method as claimed in claim 1, wherein: the detection wavelength of the DAD detector in the step (2) is 242 nm.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101846661A (en) * | 2010-05-24 | 2010-09-29 | 杭州蜂之语蜂业股份有限公司 | Method for simultaneously measuring residual quantities of lincomycin and macrolides in royal jelly |
| CN104990996A (en) * | 2015-05-19 | 2015-10-21 | 江苏出入境检验检疫局动植物与食品检测中心 | Method for detecting antibiotic residues in milk, and application thereof |
| CN106841429A (en) * | 2017-01-04 | 2017-06-13 | 华中农业大学 | The high-throughput screening method of antimicrobial DP finish in a kind of milk |
| CN109633065A (en) * | 2018-12-28 | 2019-04-16 | 沈阳出入境检验检疫局检验检疫综合技术中心 | The detection method of medicament residue in a kind of animal body |
-
2019
- 2019-08-02 CN CN201910711062.6A patent/CN110632225A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101846661A (en) * | 2010-05-24 | 2010-09-29 | 杭州蜂之语蜂业股份有限公司 | Method for simultaneously measuring residual quantities of lincomycin and macrolides in royal jelly |
| CN104990996A (en) * | 2015-05-19 | 2015-10-21 | 江苏出入境检验检疫局动植物与食品检测中心 | Method for detecting antibiotic residues in milk, and application thereof |
| CN106841429A (en) * | 2017-01-04 | 2017-06-13 | 华中农业大学 | The high-throughput screening method of antimicrobial DP finish in a kind of milk |
| CN109633065A (en) * | 2018-12-28 | 2019-04-16 | 沈阳出入境检验检疫局检验检疫综合技术中心 | The detection method of medicament residue in a kind of animal body |
Non-Patent Citations (6)
| Title |
|---|
| HUBERT PO-ON TANG ET AL: "High-throughput screening for multi-class veterinary drug residues in animal muscle using liquid chromatography/tandem mass spectrometry with on-line solid-phase extraction", 《RAPID COMMUNICATIONS IN MASS SPECTROMETRY》 * |
| 吕迁洲 等: "3种交沙霉素片剂的生物等效性研究", 《中国临床药学杂志》 * |
| 吴伟洁 等: "高效液相色谱法测定交沙霉素血药浓度", 《中国临床药学杂志》 * |
| 王茉莉 等: "HPLC法测定交沙霉素原料药中有关物质的含量", 《中国药房》 * |
| 申雪丽 等: "液相色谱-串联质谱法测定蜂蜜中8种大环内酯类药物残留的不确定度评定", 《食品安全质量检测学报》 * |
| 谢丽琪 等: "高效液相色谱串联质谱法测定牛奶中林可酰胺类和大环内酯类抗生素残留量的研究", 《分析试验室》 * |
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Application publication date: 20191231 |