CN110618115A - Method for defining effective working temperature parameter of fluorescent probe - Google Patents
Method for defining effective working temperature parameter of fluorescent probe Download PDFInfo
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- CN110618115A CN110618115A CN201910789120.7A CN201910789120A CN110618115A CN 110618115 A CN110618115 A CN 110618115A CN 201910789120 A CN201910789120 A CN 201910789120A CN 110618115 A CN110618115 A CN 110618115A
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
Abstract
The invention belongs to the technical field of analysis and test, and relates to a method for defining an effective working temperature parameter of a fluorescent probe; the method specifically comprises three steps of detecting a probe signal under the condition of temperature deviation, analyzing a response rule of a fluorescent probe to the temperature deviation and quantitatively defining the effective working temperature of the probe; according to the invention, by analyzing the response rule of the fluorescent probe signal to positive deviation and negative deviation of the temperature, a positive deviation amplitude function and a negative deviation amplitude function of the fluorescent signal are respectively constructed, so that the accurate definition of the corresponding working temperature parameter of the fluorescent probe in the allowable detection error range is realized, a reference basis can be provided for the effective working temperature range definition of the fluorescent probe in practical application, and the efficient and accurate fluorescence detection of the analyte to be detected is realized.
Description
Technical Field
The invention belongs to the technical field of analysis and test, and relates to a method for defining an effective working temperature parameter of a fluorescent probe.
Background
The fluorescent probe changes the self fluorescent characteristic after reacting with a target analyte to be detected, thereby realizing qualitative or quantitative detection of the analyte to be detected, having the advantages of high analysis sensitivity, low detection cost, simple operation and the like, and being widely applied to the fields of food detection, environmental monitoring and biological imaging. The high sensitivity of the fluorescent probe also means that the fluorescent probe is susceptible to environmental parameters of a detection system, so that the definition of the effective working environment of the fluorescent probe is crucial to the effectiveness, reliability and stability of the actual detection result of the fluorescent probe.
The test environment temperature is one of the important factors influencing the detection effectiveness of the fluorescent probe. In a laboratory environment, the fluorescent probe has high test precision after being optimized by the test environment temperature. In the practical application process of the fluorescent probe, the corresponding test environment temperature cannot be strictly consistent with the optimal test environment temperature. It is important to accurately define the temperature of the operating environment of the fluorescent probe within the allowable detection accuracy error.
The existing method for defining the effective working temperature of the fluorescent probe comprises the steps of measuring a fluorescent signal corresponding to a limited temperature value near the optimal working temperature, and comparing the fluorescent signal intensity of the limited temperature value with the fluorescent signal intensity corresponding to the optimal working temperature so as to determine the effective working temperature range corresponding to the given error. The disadvantage of this method is that the boundary of the resulting effective operating temperature range must be one of a limited number of measured temperature values, the boundary values of which cannot be precisely defined.
Disclosure of Invention
The invention aims to solve one of the problems in the prior art, and provides a method for defining the effective working temperature parameter of a fluorescent probe, thereby providing a reference basis for determining the practical application range of the fluorescent probe.
The invention relates to a method for defining effective working temperature parameters of a fluorescent probe, which specifically comprises the following three steps of detecting probe signals under the condition of temperature deviation, analyzing the response rule of the fluorescent probe to the temperature deviation and quantitatively defining the effective working temperature of the probe:
detection of probe signal under temperature excursion:
for an optimum working temperature of T0The temperature forward deviation amplitude of the fluorescent probe is set to be delta T1DEG C, negative temperature offset amplitude Delta T2DEG C; sequentially measuring the fluorescent signals of the corresponding fluorescent probes at different temperatures in the positive deviation amplitude and the negative deviation amplitude;
the detection method of the fluorescence signal corresponding to the fluorescent probe at the temperature within the positive deviation amplitude and the negative deviation amplitude comprises the following specific steps:
when the temperature is shifted in the positive direction, the detection frequency of the fluorescence signal is n +1, and the single amplification is delta T1N ℃, measuring the temperature T in sequence0，T0+ΔT1/n，T0+2ΔT1/n，……，T0+(n-1)ΔT1/n，T0+nΔT1Fluorescence signal A corresponding to/n DEG C0，A1，A2，……，An-1，An(ii) a When the temperature is shifted negatively, the detection frequency of the fluorescence signal is m, and the single amplitude is reduced by delta T2M, measuring the temperature T in sequence0-ΔT2/m，T0–2ΔT2/m，……，T0-(m-1)ΔT2/m，T0–mΔT2Fluorescence signal B corresponding to m DEG C1，B2，……，Bm-1，Bm(ii) a Wherein n and m are integers greater than 0.
Analyzing the temperature deviation response rule by the fluorescent probe:
corresponding fluorescent signals of the fluorescent probe to the optimal working temperature T at different temperatures within the positive deviation amplitude and the negative deviation amplitude of the temperature0Subtracting the corresponding fluorescence signals at the temperature of DEG C and taking an absolute value to obtain a fluorescence signal offset amplitude; temperature deviation amplitude is used as an independent variable, and fluorescence is usedThe optical signal offset amplitude is used as a dependent variable, and a fluorescence signal forward offset amplitude function Y-F is established1(X) and a negative offset magnitude function V ═ F2(U); simultaneously establishing the inverse function X-F of the forward offset amplitude function of the fluorescence signal1 -1(Y) and the inverse of the negative offset magnitude function U ═ F2 -1(V)；
The fluorescence signal offset amplitude is calculated as follows: when the temperature is shifted in the positive direction, the temperature T is adjusted0+ΔT1/n，T0+2ΔT1/n，……，T0+(n-1)ΔT1/n，T0+nΔT1Fluorescence signal A corresponding to/n DEG C1，A2，……，An-1，AnAnd a temperature of T0Fluorescent signal A of0Subtracting and taking the absolute value to obtain the temperature deviation amplitude delta T1/n，2ΔT1/n，……，(n-1)ΔT1/n，nΔT1Fluorescence signal offset amplitude | A corresponding to/n DEG C1-A0|，|A2-A0|，……，|An-1-A0|，|An-A0L, |; when the temperature is negatively shifted, the temperature T is adjusted0-ΔT2/m，T0–2ΔT2/m，……，T0-(m-1)ΔT2/m，T0–mΔT2Fluorescence signal B corresponding to m DEG C1，B2，……，Bm-1，BmAnd a temperature of T0Fluorescent signal A of0Subtracting and taking the absolute value to obtain the temperature deviation amplitude delta T2/m，2ΔT2/m，……，(m-1)ΔT2/m，mΔT2Fluorescence signal offset amplitude | B corresponding to m DEG C1-A0|，|B2-A0|，……，|Bm-1-A0|，|Bm-A0|；
Wherein n and m are integers greater than 0.
The forward offset magnitude function Y ═ F1(X) and a negative offset magnitude function V ═ F2The establishing method of (U) is specifically operated as follows: when the temperature is shifted in the positive direction, the temperature is shifted by the amplitude 0, Delta T1/n，2ΔT1/n，……，(n-1)ΔT1/n，nΔT1The/n is independent variable X, and the fluorescence signal deviation amplitude is 0, | A1-A0|，|A2-A0|，……，|An-1-A0|，|An-A0I is dependent variable Y, and a fluorescence signal forward deviation amplitude function Y is established as F1(X); when the temperature is shifted in the negative direction, the temperature is shifted by the amplitude 0, delta T2/m，2ΔT2/m，……，(m-1)ΔT2/m，mΔT2M is an independent variable U, and the fluorescence signal shifts the amplitude value 0, | B1-A0|，|B2-A0|，……，|Bm-1-A0|，|Bm-A0L is a dependent variable V, and a negative deviation amplitude function V ═ F of the fluorescence signal is established2(U)。
The positive offset amplitude function and the negative offset amplitude function are obtained through Excel linear fitting.
Quantitative definition of the effective operating temperature of the probe:
setting the positive and negative allowance errors of the fluorescence signal to obtain the optimal working temperature T of the fluorescence probe0Calculating the positive allowable maximum deviation amplitude and the negative allowable maximum deviation amplitude of the fluorescent signal by taking the corresponding fluorescent signal as a reference point;
the calculation method of the positive allowable maximum deviation amplitude and the negative allowable maximum deviation amplitude of the fluorescence signal is as follows: setting a forward allowable error C1% of the optimum operating temperature T of the fluorescent probe0Corresponding fluorescence signal A0Calculating the maximum allowable forward deviation amplitude A of the fluorescent signal as a reference point1＝|A0*C1Percent, |; set negative allowable error-C2% of the optimum operating temperature T of the fluorescent probe0Corresponding fluorescence signal A0Calculating the maximum allowable negative deviation amplitude A of the fluorescence signal as a reference point2＝|-A0*C2|
Forward allowing maximum deviation amplitude A of fluorescent signal1Substituting the inverse of the forward offset magnitude function of the signal X ═ F1 -1(Y) calculating the maximum allowable forward shift amplitude A of the fluorescence signal1Forward direction of time correspondenceOffset temperature T+＝F1-1(ΔA1) (ii) a Negative-going allowable maximum shift amplitude A of fluorescent signal2Substituting the inverse U-F of the forward offset magnitude function of the signal2 -1(V) calculating the maximum allowable negative shift amplitude A of the fluorescence signal2Time corresponding negative offset time temperature T-＝F2 -1(ΔA2) (ii) a From this, the allowable error range-C of the fluorescent probe signal was determined2％～C1% corresponding to an effective operating temperature of T0-T-～T0+T+℃。
The invention has the beneficial effects that:
according to the invention, by analyzing the response rule of the fluorescent probe signal to positive deviation and negative deviation of the temperature, a positive deviation amplitude function and a negative deviation amplitude function of the fluorescent signal are respectively constructed, so that the accurate definition of the corresponding working temperature parameter of the fluorescent probe in the allowable detection error range is realized, a reference basis can be provided for the effective working temperature range definition of the fluorescent probe in practical application, and the efficient and accurate fluorescence detection of the analyte to be detected is realized.
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FIG. 1 is a flow chart of the method of the present invention.
Detailed description of the preferred embodiments
The present invention will be described in detail below with reference to embodiments. These examples are only for illustrating the present invention and are not intended to limit the scope of the present invention. In addition, after reading the contents of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalents also fall within the scope defined by the appended claims of the present application.
In the embodiment, a copper nanocluster (CuNCs) is used as a fluorescent probe to construct a fluorescence signal positive offset amplitude function and a fluorescence signal negative offset amplitude function, and an allowable detection error range of a working temperature parameter corresponding to the copper nanocluster is defined.
Preparing a copper nano-cluster fluorescent probe: 32mg of CuSO was taken4Added to 20g of water, 2mL of NaOH solution (0.5M) and 20mL of ascorbic acid were addedAnd (3) stirring the solution (0.1M) in a water bath for 15h under the conditions that the pH value is adjusted to 8.0-9.0 and the temperature is 50 ℃ to obtain a copper nano-cluster solution stock solution, and dialyzing the solution for 24h in deionized water by using a dialysis bag (1000D) for purification to obtain the copper nano-cluster fluorescent probe with the excitation wavelength of 365nm and the emission wavelength of 445 nm.
The method for defining the effective working temperature parameter of the copper nano-cluster fluorescent probe comprises the following three steps:
step one, detecting a copper nanocluster fluorescence signal under the condition of temperature deviation:
step 1.1, setting an environmental temperature value:
the optimal working temperature T of the known copper nanocluster fluorescent probe0At 25 deg.C, the effective operating temperature range of the probe needs to be determined, and then the temperature forward deviation amplitude Delta T is set1At 15 deg.C, a negative temperature excursion DeltaT2At 25 ℃;
step 1.2, detecting a copper nano-cluster fluorescence signal:
the detection frequency of the fluorescence signal is 4, the single amplification is 5 ℃, and the corresponding fluorescence intensities of 800, 788, 773 and 761 are measured at the temperatures of 25, 30, 35 and 40 ℃ in sequence; when the temperature is shifted negatively, the detection frequency of the fluorescence signal is 5, the single reduction is 5 ℃, and the fluorescence intensities 813, 821, 829, 835 and 842 corresponding to the temperatures of 20, 15, 10, 5 and 0 ℃ are measured sequentially;
step two, analyzing the temperature deviation response rule of the copper nanocluster comprises the following processes:
step 2.1, calculating the fluorescence signal offset amplitude corresponding to the temperature offset amplitude:
when the temperature is shifted forwards, subtracting the fluorescence intensity 788, 773, 761 corresponding to the temperature of 30, 35, 40 ℃ from the fluorescence intensity 800 corresponding to the temperature of 25 ℃ and taking the absolute value to obtain the fluorescence signal shift amplitude values 12, 27, 39 corresponding to the temperature shift amplitudes 5, 10, 15 ℃; when the temperature is negatively shifted, subtracting the fluorescence intensity 800 at 25 ℃ from the fluorescence signals 813, 821, 829, 835, 842 at 20, 15, 10, 5, 0 ℃ and taking the absolute value to obtain the fluorescence signal shift amplitudes 13, 21, 29, 35, 42 at 5, 10, 15, 20, 25 ℃;
step 2.2, regression of the copper nanocluster fluorescence signal offset amplitude function:
when the temperature is positively deviated, taking the temperature deviation amplitude 0, 5, 10, 15 ℃ as an independent variable X and the fluorescence signal deviation amplitude 0, 12, 27, 39 as a dependent variable Y, and performing linear fitting by using Excel, wherein the intercept is set to be 0 to obtain a fluorescence signal forward deviation amplitude function Y ═ F1(X) ═ 2.6143X; when the temperature is negatively deviated, taking the temperature deviation amplitude of 0, 5, 10, 15, 20 and 25 ℃ as an independent variable U, taking the fluorescence signal deviation amplitude of 0, 13, 21, 29, 35 and 42 as a dependent variable V, and performing linear fitting by using Excel, wherein the intercept is set to be 0, and obtaining a fluorescence signal negative deviation amplitude function V ═ F2(U)＝1.7891U；
Step 2.3, calculating the offset amplitude inverse function of the copper nanocluster fluorescent signal:
obtaining the inverse function X-F of the forward shift amplitude function of the optical signal according to the forward shift amplitude function Y-2.6143X of the fluorescent signal, wherein X-Y/2.6143-0.3825Y1 -1(Y) ═ 0.3825Y; according to the negative shift amplitude function V of the fluorescence signal 1.7891U, wherein U is V/1.7891 is 0.5589V, the inverse function U is F of the negative shift amplitude function of the fluorescence signal2 -1(V)＝0.5589V；
Step three, the quantitative definition of the effective working temperature of the copper nanocluster comprises the following steps:
step 3.1, setting of allowable working error of fluorescence signal:
setting a forward allowable error C1% is 0.5%, and the maximum allowable forward deviation amplitude delta A of the fluorescence signal is calculated by taking the fluorescence intensity 800 corresponding to the optimal working temperature of the fluorescence probe of 25 ℃ as a reference point1＝|A0*C1% is 800 × 0.5% | 4; setting a negative allowable error C2% is-0.5%, and the fluorescence intensity 800 corresponding to the optimal working temperature of 25 ℃ of the fluorescence probe is taken as a reference point to calculate the negative allowable maximum amplitude delta A of the fluorescence signal2＝|-A0*C2％|＝|-800×0.5％|＝4；
And 3.2, calculating the effective working temperature of the probe:
forward allowing maximum shift of fluorescent signalAmplitude Δ A1Substituting 4 into the inverse function X F of the temperature at positive offset1 -1(Y) calculating the maximum allowable forward deviation amplitude of the fluorescence signal as Δ A1Forward offset time temperature T corresponding to 4 hours+＝F1-1(ΔA1) 0.3825 × 4 ═ 1.53 ℃; negative-going allowable maximum shift amplitude A of fluorescent signal2Substituting 4 into the inverse function of temperature in negative-going signal, U-F2 -1(V) calculating the maximum allowable negative shift amplitude of the fluorescence signal as Δ A2Negative offset temperature T corresponding to 4-＝F2 -1(ΔA2) 0.5589 × 4 ═ 2.24 ℃; therefore, the allowable error range of the fluorescent probe signal is determined to be-0.5%, and the corresponding effective working temperature is 22.76-26.53 ℃.
Measuring the error of the copper nanocluster within an effective working range of 22.76-26.53 ℃, and obtaining the fluorescence intensity of 803, 800 and 798 under 365nm excitation at 23, 24, 25 and 26 ℃, wherein the measured errors are respectively: 0.375%, 0%, 0.25%, all < 0.5%, indicating that the method for defining the effective operating temperature parameter of the fluorescent probe of the present invention is feasible.
Claims (6)
1. A method for defining effective working temperature parameters of a fluorescent probe is characterized by comprising the following steps:
detection of probe signal under temperature excursion:
for an optimum working temperature of T0The temperature forward deviation amplitude of the fluorescent probe is set to be delta T1DEG C, negative temperature offset amplitude Delta T2DEG C; sequentially measuring the fluorescent signals of the corresponding fluorescent probes at different temperatures in the positive deviation amplitude and the negative deviation amplitude;
analyzing the temperature deviation response rule by the fluorescent probe:
corresponding fluorescent signals of the fluorescent probe to the optimal working temperature T at different temperatures within the positive deviation amplitude and the negative deviation amplitude of the temperature0Subtracting the corresponding fluorescence signals at the temperature of DEG C and taking an absolute value to obtain a fluorescence signal offset amplitude; temperature deviation amplitude is used as an independent variable, and fluorescence signal deviation amplitude is usedThe value is a dependent variable, and a forward shift amplitude function Y ═ F of the fluorescence signal is established1(X) and a negative offset magnitude function V ═ F2(U); simultaneously establishing the inverse function X-F of the forward offset amplitude function of the fluorescence signal1 -1(Y) and the inverse of the negative offset magnitude function U ═ F2 -1(V)；
Quantitative definition of the effective operating temperature of the probe:
setting the positive and negative allowance errors of the fluorescence signal to obtain the optimal working temperature T of the fluorescence probe0Calculating the positive allowable maximum deviation amplitude and the negative allowable maximum deviation amplitude of the fluorescent signal by taking the corresponding fluorescent signal as a reference point;
respectively substituting the positive allowable maximum deviation amplitude and the negative allowable maximum deviation amplitude of the fluorescent signal into the inverse function of the positive deviation amplitude function and the inverse function of the negative deviation amplitude function of the signal, and calculating the corresponding positive deviation temperature T when the positive allowable maximum deviation amplitude of the fluorescent signal is reached+Temperature T at negative offset corresponding to maximum allowable negative offset-(ii) a Thereby determining the corresponding effective working temperature T within the allowable error range of the fluorescent probe signal0-T-～T0+T+℃。
2. The method of claim 1, wherein the method for detecting the fluorescence signal of the fluorescence probe at the temperature within the positive offset amplitude and the negative offset amplitude comprises the following steps:
when the temperature is shifted in the positive direction, the detection frequency of the fluorescence signal is n +1, and the single amplification is delta T1N ℃, measuring the temperature T in sequence0，T0+ΔT1/n，T0+2ΔT1/n，……，T0+(n-1)ΔT1/n，T0+nΔT1Fluorescence signal A corresponding to/n DEG C0，A1，A2，……，An-1，An(ii) a When the temperature is shifted negatively, the detection frequency of the fluorescence signal is m, and the single amplitude is reduced by delta T2M, measuring the temperature T in sequence0-ΔT2/m，T0–2ΔT2/m，……，T0-(m-1)ΔT2/m，T0–mΔT2Fluorescence signal B corresponding to m DEG C1，B2，……，Bm-1，Bm(ii) a Wherein n and m are integers greater than 0.
3. The method of claim 1, wherein the fluorescence signal offset amplitude is calculated as follows:
when the temperature is shifted in the positive direction, the temperature T is adjusted0+ΔT1/n，T0+2ΔT1/n，……，T0+(n-1)ΔT1/n，T0+nΔT1Fluorescence signal A corresponding to/n DEG C1，A2，……，An-1，AnAnd a temperature of T0Fluorescent signal A of0Subtracting and taking the absolute value to obtain the temperature deviation amplitude delta T1/n，2ΔT1/n，……，(n-1)ΔT1/n，nΔT1Fluorescence signal offset amplitude | A corresponding to/n DEG C1-A0|，|A2-A0|，……，|An-1-A0|，|An-A0L, |; when the temperature is negatively shifted, the temperature T is adjusted0-ΔT2/m，T0–2ΔT2/m，……，T0-(m-1)ΔT2/m，T0–mΔT2Fluorescence signal B corresponding to m DEG C1，B2，……，Bm-1，BmAnd a temperature of T0Fluorescent signal A of0Subtracting and taking the absolute value to obtain the temperature deviation amplitude delta T2/m，2ΔT2/m，……，(m-1)ΔT2/m，mΔT2Fluorescence signal offset amplitude | B corresponding to m DEG C1-A0|，|B2-A0|，……，|Bm-1-A0|，|Bm-A0|；
Wherein n and m are integers greater than 0.
4. The effective operating temperature of a fluorescent probe of claim 1Method for defining parameters, characterized in that said forward offset magnitude function Y ═ F1(X) and a negative offset magnitude function V ═ F2The establishing method of (U) is specifically operated as follows:
when the temperature is shifted in the positive direction, the temperature is shifted by the amplitude 0, Delta T1/n，2ΔT1/n，……，(n-1)ΔT1/n，nΔT1The/n is independent variable X, and the fluorescence signal deviation amplitude is 0, | A1-A0|，|A2-A0|，……，|An-1-A0|，|An-A0I is dependent variable Y, and a fluorescence signal forward deviation amplitude function Y is established as F1(X); when the temperature is shifted in the negative direction, the temperature is shifted by the amplitude 0, delta T2/m，2ΔT2/m，……，(m-1)ΔT2/m，mΔT2M is an independent variable U, and the fluorescence signal shifts the amplitude value 0, | B1-A0|，|B2-A0|，……，|Bm-1-A0|，|Bm-A0L is a dependent variable V, and a negative deviation amplitude function V ═ F of the fluorescence signal is established2(U)。
5. The method of claim 1, wherein the positive shift amplitude function and the negative shift amplitude function are obtained by Excel linear fitting.
6. The method of claim 1, wherein the maximum allowable positive and negative offset magnitudes of the fluorescence signal are calculated as follows: setting a forward allowable error C1% of the optimum operating temperature T of the fluorescent probe0Corresponding fluorescence signal A0Calculating the maximum allowable forward deviation amplitude A of the fluorescent signal as a reference point1＝|A0*C1Percent, |; set negative allowable error-C2% of the optimum operating temperature T of the fluorescent probe0Corresponding fluorescence signal A0Calculating the maximum allowable negative deviation amplitude A of the fluorescence signal as a reference point2＝|-A0*C2％|。
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103674927A (en) * | 2013-12-18 | 2014-03-26 | 江苏大学 | Correction method in Raman spectroscopy quantitative detection under temperature fluctuation condition |
| CN109100322A (en) * | 2018-08-17 | 2018-12-28 | 江苏大学 | Food near-infrared spectrum rapid detection method and portable detector based on temperature self-correcting |
-
2019
- 2019-08-26 CN CN201910789120.7A patent/CN110618115B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103674927A (en) * | 2013-12-18 | 2014-03-26 | 江苏大学 | Correction method in Raman spectroscopy quantitative detection under temperature fluctuation condition |
| CN109100322A (en) * | 2018-08-17 | 2018-12-28 | 江苏大学 | Food near-infrared spectrum rapid detection method and portable detector based on temperature self-correcting |
Non-Patent Citations (2)
| Title |
|---|
| 邹小波等: "乙醇水溶液体积分数和温度的近红外激光拉曼光谱研究", 《江苏大学学报》 * |
| 邹小波等: "基于高光谱图像的香醋醋醅均匀性快速判别", 《农业机械学报》 * |
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