CN110612979A - Disease-resistant growth-promoting composite bacillus water dispersible granule as well as preparation method and application thereof - Google Patents
Disease-resistant growth-promoting composite bacillus water dispersible granule as well as preparation method and application thereof Download PDFInfo
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- CN110612979A CN110612979A CN201910968886.1A CN201910968886A CN110612979A CN 110612979 A CN110612979 A CN 110612979A CN 201910968886 A CN201910968886 A CN 201910968886A CN 110612979 A CN110612979 A CN 110612979A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
- A01N25/14—Powders or granules wettable
Abstract
The invention discloses a disease-resistant growth-promoting composite bacillus water dispersible granule which comprises composite bacillus, a carrier and an auxiliary agent, wherein the composite bacillus comprises stratospheric bacillus SC-12 and bacillus amyloliquefaciens BZ6-1, and the ratio of the viable count of the stratospheric bacillus SC-12 to the viable count of the bacillus amyloliquefaciens BZ6-1 is 2: 1. The invention also discloses a preparation method of the disease-resistant growth-promoting composite bacillus water dispersible granule, which comprises the steps of centrifuging mother liquor obtained by mixed culture of bacillus amyloliquefaciens BZ6-1 and bacillus stratosphericus SC-12, drying the mixed mother liquor to prepare bacterial powder, adding 1% of bacterial powder into a carrier, a wetting agent, a dispersing agent, a disintegrating agent and a binder, uniformly mixing, adding water, kneading, granulating, screening and drying to obtain the product. The disease-resistant growth-promoting composite bacillus water dispersible granule can be used for salt-resistant growth promotion, biocontrol and disease resistance of plants, and can improve the yield and quality of the plants.
Description
Technical Field
The invention relates to a disease-resistant growth-promoting composite bacillus water dispersible granule as well as a preparation method and application thereof, belonging to the technical field of agricultural microorganisms.
Background
China is a big country of facility agriculture, farmers generally rely on big water to increase crop yield in order to pursue higher economic benefit, and secondary salinization of facility soil and serious occurrence of soil-borne diseases are caused. In recent years, researches on improving crop yield, preventing diseases and improving tolerance to various environments by utilizing rhizosphere salt-tolerant growth-promoting bacteria and biocontrol bacteria are one of the hot spots of researches on agricultural application microorganisms in recent years. The existing biological agent has single action and poor action effect and cannot meet the production requirement.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a disease-resistant growth-promoting composite bacillus water dispersible granule as well as a preparation method and application thereof, wherein a bacillus pumilus SC-12 strain and a bacillus amyloliquefaciens BZ6-1 strain are compositely cultured to construct a composite microbial inoculum to exert the efficacy of the composite microbial inoculum in a synergistic manner, and a multifunctional biological microbial inoculum with double effects of salt resistance, growth promotion, biological control and disease resistance is developed.
In order to solve the technical problems, the invention provides a disease-resistant growth-promoting composite bacillus water dispersible granule which comprises composite bacillus, a carrier and an auxiliary agent, wherein the composite bacillus comprises bacillus pumilus SC-12 and bacillus amyloliquefaciens BZ6-1, and the ratio of the viable count of the bacillus pumilus SC-12 to the viable count of the bacillus amyloliquefaciens BZ6-1 is 2: 1.
Bacillus pumilus SC-12(Bacillus pumilus) is preserved in the China general microbiological culture Collection center of China Committee for culture Collection of microorganisms, and the preservation address is as follows: west road No. 1 of north chen of chaoyang district, beijing, institute of microbiology of china academy of sciences, the date of deposit is No. 5 month 15 in 2013, the number of deposit is: CGMCC NO. 7622.
Bacillus amyloliquefaciens BZ6-1(Bacillus amyloliquefaciens) is deposited in a depositary designated by the national intellectual property office, wherein the deposition date is 2009, 01 and 22 days, and the name of the depositary is as follows: china general microbiological culture Collection center, preservation Address: the microbial research institute of China academy of sciences, university of Inward Yangtze district, Beijing, has a preservation number: CGMCC No. 2892.
Preferably, the auxiliaries include wetting agents, dispersants, disintegrants and binders.
Preferably, the carrier is one or more of talcum powder, calcium carbonate, ammonium sulfate and diatomite.
Preferably, the content of the bacillus compositus is 1%, the content of the wetting agent is 6%, the content of the dispersing agent is 6%, the content of the disintegrating agent is 4%, the content of the binder is 4%, and the balance of the carrier is 100%.
Preferably, the wetting agent is one or more of sodium dodecyl sulfate, sodium dodecyl benzene sulfonate and saponin powder; the dispersing agent is one or more of sodium lignosulphonate, sodium carboxymethyl cellulose and sodium tripolyphosphate; the disintegrating agent is one or more of calcium chloride, ammonium sulfate and sodium chloride; the binder is one or more of polyvinyl alcohol, polyethylene glycol and soluble starch.
Preferably, the carrier is diatomite, the wetting agent is sodium dodecyl sulfate, the dispersing agent is sodium carboxymethyl cellulose, the disintegrating agent is ammonium sulfate, and the binder is polyvinyl alcohol.
The invention also provides a preparation method of the disease-resistant growth-promoting composite bacillus water dispersible granule, which comprises the steps of centrifuging mother liquor obtained by mixed culture of bacillus amyloliquefaciens BZ6-1 and bacillus pumilus SC-12, drying the mixed mother liquor to prepare bacterial powder, adding 1% of bacterial powder into a carrier, a wetting agent, a dispersing agent, a disintegrating agent and a binder, uniformly mixing, adding water, kneading, granulating, screening and drying to obtain the product.
Preferably, the particle size of the screened mesh is 0.8mm, the drying temperature is 40 ℃, and the drying time is 45 min.
The invention also provides application of the disease-resistant growth-promoting composite bacillus water dispersible granule in salt-tolerant growth promotion, biocontrol and disease resistance of plants.
The invention also provides application of the disease-resistant growth-promoting composite bacillus water dispersible granule in improving the yield and quality of plants.
The invention achieves the following beneficial effects:
(1) the ACC deaminase specific activity of the Bacillus pumilus SC-12 strain (Bacillus pumilus) reaches 0.087U/mg, and the Bacillus pumilus SC-12 strain has strong siderophore capacity, and can remarkably promote the germination rate and the growth of buds of plants under the stress conditions of 100mmol/L nitrate and 200 mmol/L nitrate; the bacillus amyloliquefaciens BZ6-1 strain (Bacillus amyloliquefaciens) has stronger inhibiting effect on bacterial diseases such as ralstonia solanacearum and the like and fungal diseases such as rice sheath blight bacteria and rice blast bacteria and the like, and has wider antibacterial spectrum; the invention compositely cultures the Bacillus pumilus SC-12 strain and the Bacillus amyloliquefaciens BZ6-1 strain to construct a composite microbial inoculum to cooperatively exert the efficacy of the composite microbial inoculum and develop a multifunctional biological microbial inoculum which achieves the double effects of salt tolerance, growth promotion, biocontrol and disease resistance.
(2) When the water dispersible granule is used, the water dispersible granule is put into water to be rapidly disintegrated and dispersed to form a high-suspension system, and has the advantages of high dispersibility, high effectiveness, high stability and environmental friendliness.
(3) The addition of the auxiliary agent can enhance the control effect of the water dispersible granule, improve the physicochemical property and the service performance of the water dispersible granule, reduce the dosage of the water dispersible granule, save the cost and protect the environment.
(4) The spore content of the compound water dispersible granule prepared by the invention is 2.5 multiplied by 108cfu·g-1The suspension percentage is 79.3%, the pH value is 6.8, the water content is 4.5%, the wetting time is 19.6s, the disintegration time is 86.4s, the granule strength is moderate, and the application of the water dispersible granule improves the yield and the quality of plants.
Drawings
FIG. 1 is a process flow chart of the disease-resistant growth-promoting composite bacillus water dispersible granule of the invention;
FIG. 2 is a graph showing the effect of different storage modes on the activity of bacillus water dispersible granules.
Detailed Description
The invention is further described below with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and the protection scope of the present invention is not limited thereby.
Example 1
The invention provides a disease-resistant growth-promoting composite bacillus water dispersible granule which comprises composite bacillus, a carrier and an auxiliary agent, wherein the composite bacillus comprises bacillus pumilus SC-12 and bacillus amyloliquefaciens BZ6-1, and the ratio of the viable count of the bacillus pumilus SC-12 to the viable count of the bacillus amyloliquefaciens BZ6-1 is 2: 1.
Bacillus pumilus SC-12(Bacillus pumilus) is preserved in the China general microbiological culture Collection center of China Committee for culture Collection of microorganisms, and the preservation address is as follows: west road No. 1 of north chen of chaoyang district, beijing, institute of microbiology of china academy of sciences, the date of deposit is No. 5 month 15 in 2013, the number of deposit is: CGMCC NO. 7622.
Bacillus amyloliquefaciens BZ6-1(Bacillus amyloliquefaciens) is deposited in a depositary designated by the national intellectual property office, wherein the deposition date is 2009, 01 and 22 days, and the name of the depositary is as follows: china general microbiological culture Collection center, preservation Address: the microbial research institute of China academy of sciences, university of Inward Yangtze district, Beijing, has a preservation number: CGMCC No. 2892.
The auxiliary agent comprises a wetting agent, a dispersing agent, a disintegrating agent and a binder. The wetting agent can be one or more of sodium dodecyl sulfate, sodium dodecyl benzene sulfonate and saponin powder; the dispersant can be one or more of sodium lignosulphonate, sodium carboxymethyl cellulose and sodium tripolyphosphate; the disintegrant can be one or more of calcium chloride, ammonium sulfate and sodium chloride; the binder can be one or more of polyvinyl alcohol, polyethylene glycol and soluble starch. The carrier can be one or more of talcum powder, calcium carbonate, ammonium sulfate and diatomite.
As shown in figure 1, the preparation method of the disease-resistant growth-promoting composite bacillus water dispersible granule comprises the following steps: centrifuging mother liquor obtained by mixed culture of bacillus amyloliquefaciens BZ6-1 and bacillus pumilus SC-12, drying the mixed mother liquor to prepare bacterial powder, adding 1% of bacterial powder into a carrier, a wetting agent, a dispersing agent, a disintegrating agent and a binder, uniformly mixing, adding water, kneading, granulating, screening and drying to obtain the product.
The optimal formula of the disease-resistant growth-promoting composite bacillus water dispersible granule obtained by research is as follows: the carrier is diatomite, the wetting agent is sodium dodecyl sulfate, the dispersing agent is sodium carboxymethyl cellulose, the disintegrating agent is ammonium sulfate, and the binder is polyvinyl alcohol. The content of the composite bacillus is 1%, the content of the wetting agent is 6%, the content of the dispersing agent is 6%, the content of the disintegrating agent is 4%, the content of the binder is 4%, and the balance of the carrier is 100%. The optimum conditions for granulation were: 0.8mm sieve mesh particle size, 40 ℃ drying temperature and 45min drying time. Under these conditions: the spore content of the prepared composite bacillus water dispersible granule is 2.5 multiplied by 108cfu g-1, suspension rate 79.3%, pH value 6.8, water content 4.5%, wetting time 19.6s, disintegration time 86.4s, and moderate particle strength.
Analyzing and determining the quality index of the preparation: the prepared disease-resistant growth-promoting composite bacillus water dispersible granule is determined according to national standard of the people's republic of China. The suspension rate is determined by reference GB/T14825-2006, the pH value is determined by reference GB/T1601-1993, the moisture is determined by reference GB/T1600-2001, and the wetting time is determined by reference GB/T5451-2001.
TABLE 1 determination of quality index of Water dispersible granule
As can be seen from Table 1, the indexes of the composite bacillus water dispersible granule developed in the experiment are in accordance with the national standard, and the wettability, the dispersibility and the disintegrability of the composite bacillus water dispersible granule are far higher than the national standard.
And (3) determination of storage stability: the prepared water dispersible granules are stored under the conditions of refrigeration at 4 ℃ and room temperature (25 ℃), the bacteria content of a sample is measured every 15 days, the measuring time is 90 days, and the storage stability of the preparation is evaluated.
As shown in FIG. 2, the storage stability of the bacillus water dispersible granule is determined by the following steps: when the water dispersible granule is stored in a refrigerator at 4 ℃, the activity of the microbial inoculum is obviously higher than that of the microbial inoculum stored at room temperature, and after the water dispersible granule is stored for 90 days, the survival rate of the microbial inoculum is 29.99% at room temperature; and the spore germination rate is 55.72% under the condition of refrigeration at 4 ℃. Therefore, it can be found from the experiment that: the low temperature is beneficial to the preservation of the strains and the prolonging of the shelf life, so that the bacillus BZ6-1 and SC-12 dispersible granules can be better applied to production.
Example 2
The test is carried out in a plastic greenhouse of a Yangzhou Gaoyou vegetable base in Jiangsu province in 2019 in 3-8 months. By adopting a random block design, setting conventional compound fertilizer treatment (CK), 1 kg/mu (A) of compound fertilizer + composite water dispersible granules, 2 kg/mu (B) of compound fertilizer + composite water dispersible granules, 4 kg/mu (C) of compound fertilizer + composite water dispersible granules and an organic fertilizer (D), carrying out conventional management on the pepper after field planting in 18 days in 3 months, wherein the treatment is repeated for 3 times, and the repeated area is 15m2. Recording the weight of the pepper in the whole growth period of each cell, and respectively measuring the VC, protein, soluble sugar content and other quality related indexes of the pepper by taking three pepper plants in each cell at the later stage of pepper maturation. VC content is determined by 2, 6-dichlorophenol indophenol titration method, protein content is determined by Coomassie brilliant blue method, and soluble sugar content is determined by spectrophotometry.
TABLE 2 determination of quality index of water dispersible granule
As can be seen from Table 2, the yield of the pepper treated by the composite water dispersible granules is obviously higher than the yield of the pepper treated by the single application of the compound fertilizer and the single application of the organic fertilizer, wherein the yield of the compound fertilizer and the composite water dispersible granules is obviously increased by 16.26 percent compared with the conventional compound fertilizer treatment and is obviously increased by 9.06 percent compared with the single application of the organic fertilizer. The VC content, the soluble sugar content and the protein content of the pepper fruits are remarkably increased by the treatment of the composite bacillus water dispersible granule. The content of VC is increased along with the increase of the content of the water dispersible granules, the VC content of 1 kg/mu compound fertilizer and compound water dispersible granules is higher than that of 2 kg/mu compound fertilizer and compound water dispersible granules and 4 kg/mu compound fertilizer and compound water dispersible granules, and the difference is obvious; the protein and the soluble sugar are reduced along with the increase of the water dispersible granules, and the content of the protein and the soluble sugar in the treatment of 1 kg/mu compound fertilizer and compound water dispersible granules is lower than that in the treatment of 2 kg/mu compound fertilizer and compound water dispersible granules and that in the treatment of 4 kg/mu compound fertilizer and compound water dispersible granules, but the difference is not obvious. Comprehensively, the treatment of 1 kg/mu of the compound fertilizer and the compound water dispersible granules is the most suitable treatment, and is beneficial to improving the yield and the quality of the pepper.
The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, several modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.
Claims (10)
1. The disease-resistant growth-promoting composite bacillus water dispersible granule is characterized by comprising composite bacillus, a carrier and an auxiliary agent, wherein the composite bacillus comprises bacillus pumilus SC-12 and bacillus amyloliquefaciens BZ6-1, and the ratio of the viable count of the bacillus pumilus SC-12 to the viable count of the bacillus amyloliquefaciens BZ6-1 is 2: 1.
2. The disease-resistant growth-promoting composite bacillus water dispersible granule as claimed in claim 1, wherein the auxiliary agent comprises a wetting agent, a dispersing agent, a disintegrating agent and a binder.
3. The disease-resistant growth-promoting composite bacillus water dispersible granule according to claim 1, wherein the carrier is one or more of talcum powder, calcium carbonate, ammonium sulfate and diatomite.
4. The disease-resistant growth-promoting composite bacillus water dispersible granule according to claim 2, wherein the content of the composite bacillus is 1%, the content of the wetting agent is 6%, the content of the dispersing agent is 6%, the content of the disintegrating agent is 4%, the content of the binder is 4%, and the balance of the carrier is up to 100%.
5. The disease-resistant growth-promoting composite bacillus water dispersible granule according to claim 2, wherein the wetting agent is one or more of sodium dodecyl sulfate, sodium dodecyl benzene sulfonate and saponin powder; the dispersing agent is one or more of sodium lignosulphonate, sodium carboxymethyl cellulose and sodium tripolyphosphate; the disintegrating agent is one or more of calcium chloride, ammonium sulfate and sodium chloride; the binder is one or more of polyvinyl alcohol, polyethylene glycol and soluble starch.
6. The disease-resistant growth-promoting composite bacillus water dispersible granule according to claim 2, wherein the carrier is diatomite, the wetting agent is sodium dodecyl sulfate, the dispersing agent is sodium carboxymethyl cellulose, the disintegrating agent is ammonium sulfate, and the binder is polyvinyl alcohol.
7. A preparation method of a disease-resistant growth-promoting composite bacillus water dispersible granule is characterized in that mother liquor obtained by mixed culture of bacillus amyloliquefaciens BZ6-1 and bacillus pumilus SC-12 is centrifuged, the mixed mother liquor is dried to prepare bacterial powder, 1% of the bacterial powder is added into a carrier, a wetting agent, a dispersing agent, a disintegrating agent and a binder, the bacterial powder is uniformly mixed and added with water to be kneaded, and the product is obtained through granulation, screening and drying.
8. The preparation method of the disease-resistant growth-promoting composite bacillus water dispersible granule according to claim 7, wherein the particle size of a screened sieve pore is 0.8mm, the drying temperature is 40 ℃, and the drying time is 45 min.
9. The disease-resistant growth-promoting composite bacillus water dispersible granule according to any one of claims 1-6 or the application of the disease-resistant growth-promoting composite bacillus water dispersible granule prepared by any one of claims 7-8 in salt tolerance growth promotion and biocontrol disease resistance of plants.
10. The application of the disease-resistant growth-promoting composite bacillus water dispersible granule according to any one of claims 1 to 6 or the disease-resistant growth-promoting composite bacillus water dispersible granule prepared by the method of any one of claims 7 to 8 in improving the yield and quality of plants.
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| Publication number | Priority date | Publication date | Assignee | Title |
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Application publication date: 20191227 |