CN110591916A

CN110591916A - Cell resuscitator

Info

Publication number: CN110591916A
Application number: CN201910957690.2A
Authority: CN
Inventors: 陈龙刚
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-10-10
Filing date: 2019-10-10
Publication date: 2019-12-20

Abstract

The invention discloses a cell resuscitator, which relates to the field of cell culture and comprises a base, an adjusting mechanism, a cryopreservation tube tray, a water bath box and a control box, wherein the adjusting mechanism, the water bath box and the control box are all fixedly arranged on the base, the adjusting mechanism comprises a rotary adjusting component, a lifting adjusting component and an extension adjusting component, the lifting adjusting component is fixedly arranged on the rotary adjusting component, the extension adjusting component is fixedly arranged on the lifting adjusting component, the cryopreservation tube tray is fixedly arranged on the extension adjusting component, a separation water seepage plate, a stirring component and a heater are arranged on the water bath box, the separation water seepage plate is fixedly arranged on the peripheral inner wall of the water bath box, the heater is fixedly arranged at the side of the water bath box above the base, the output end of the heater extends to the inner bottom end of the water bath box, and the cell resuscitator can semi-automatically resuscitate cells in a plurality of cryopreservation tubes simultaneously, saves a large amount of time, and has high efficiency and practicability.

Description

Cell resuscitator

Technical Field

The invention relates to the field of cell culture, in particular to a cell resuscitator.

Background

At present, the important mode for preserving cell biological resources is liquid nitrogen freezing, when cells are preserved in a freezing way, a freezing tube filled with cell suspension is generally sealed by a sealing film and then is placed in a liquid nitrogen tank below 150 ℃ below zero for preservation, when the experiment needs, the cells are taken out of the liquid nitrogen and are recovered, when the cells are recovered, the best operation is to rapidly re-warm the just taken out freezing cells to 37 ℃, so that the cells rapidly pass through the temperature of-5 ℃ to 0 ℃ where the cells are most easily damaged, and the cells are prevented from being damaged by ice crystal recrystallization and die. In practical operation, an experimenter often uses tweezers to clamp a cell cryopreservation tube, and then vigorously shakes the tube in a water bath pan to rapidly thaw cells. However, in this way, only a few cells in the cryopreserved tubes can be thawed and thawed at a time, a large amount of resuscitating operation cannot be performed at one time, a large amount of time is wasted, and if the water temperature in the water bath is too high during manual thawing, the cells can be scalded by the water used for thawing the cells carelessly.

Disclosure of Invention

The invention aims to provide a cell resuscitator, which solves the problems that the manual thawing mode in the background technology can only thaw and resuscitate cells in a plurality of cryopreservation tubes each time, a large amount of time can not be wasted due to the fact that a large amount of resuscitation operation can not be performed at one time, and the cells can be scalded by water used for thawing the cells due to carelessness if the water temperature in a water bath is too high in the manual thawing process.

The invention provides a cell resuscitator, which comprises a base, an adjusting mechanism, a cryopreservation pipe tray, a water bath box and a control box, wherein the adjusting mechanism, the water bath box and the control box are fixedly arranged on the base, the adjusting mechanism can place the cryopreservation pipe tray into the water bath box, the adjusting mechanism comprises a rotary adjusting component, a lifting adjusting component and an extension adjusting component, the rotary adjusting component is fixedly arranged on the base, the lifting adjusting component is fixedly arranged on the rotary adjusting component, the extension adjusting component is fixedly arranged on the lifting adjusting component, the cryopreservation pipe tray is fixedly arranged on the extension adjusting component, a separation water seepage plate, a stirring component and a heater are arranged on the water bath box, the separation water seepage plate is fixedly arranged on the peripheral inner wall of the water bath box, the stirring component is fixedly arranged on the water bath box and is positioned below the separation water seepage plate, the heater is fixed to be set up the side of base top water bath, the output of heater extends to the inside bottom of water bath.

Further, the rotation adjusting assembly comprises an installation box, a rotating motor, a rotating gear, a rotating shaft, a linkage gear and a rotary disc, the installation box is internally of a hollow structure, the installation box is fixedly arranged on the base, the rotating motor is fixedly arranged on the inner bottom wall of the installation box, the rotating gear is fixedly arranged on an output shaft of the rotating motor, the rotating shaft is rotatably arranged on the installation box, the linkage gear is fixedly arranged at the middle section position of the rotating shaft in the installation box, the linkage gear is meshed with the rotating gear, and the rotary disc is fixedly arranged at the top end of the outer portion of the rotating shaft.

Further, the lift adjustment subassembly includes bracing piece, lifter, lift cylinder and lifter plate, the bracing piece is hollow structure, the bracing piece is fixed to be set up the carousel top, the lift cylinder is fixed to be set up on the interior diapire of bracing piece, the lifter is located inside the bracing piece, the lifter with sliding fit between the bracing piece, the bottom of lifter with the output fixed connection of lift cylinder, the lifter is rectangular shape panel, the fixed setting of one end of lifter is in the lifter top.

Further, extend the adjusting part and include electric putter, guide holder, guide bar and U type frame, electric putter is fixed to be set up on the other end of lifter plate, electric putter's output runs through affiliated lifter plate, the guide holder with the guide bar all is equipped with two, two the guide holder is all fixed to be set up on the lifter plate and be located respectively electric putter's both sides, two the guide bar sets up respectively two on the guide holder, two the guide bar respectively with two sliding fit between the guide holder, two the one end of guide bar all runs through the lifter plate, the fixed setting in bottom of U type frame electric putter's output and two guide bar serve.

Further, the cryopreservation pipe bearing tray is fixedly connected with two ends of the U-shaped frame, a plurality of placing grooves are formed in the cryopreservation pipe bearing tray, each placing groove is internally provided with a same limiting component, each limiting component comprises four expansion springs and four limiting blocks, one ends of the four expansion springs are fixedly arranged on the peripheral side walls of the placing grooves, and the four limiting blocks are respectively fixedly arranged at the other ends of the four expansion springs.

Further, the stirring subassembly includes protection casing, agitator motor, stirring belt, two (mixing) shafts, two stirring gears and a plurality of stirring leaf, the protection casing cover is established one side on the water bath, agitator motor is fixed to be set up in the protection casing, two the (mixing) shaft rotates the setting side by side on the water bath, two the both ends of (mixing) shaft all run through the water bath lateral wall is and extend to outside the water bath, two stirring gear is fixed respectively and is set up on one end of two (mixing) shafts, stirring belt cover is established on agitator motor's output shaft and one of them (mixing) shaft serve, a plurality of stirring leaf interval setting respectively is on two (mixing) shafts, two stirring epaxial stirring leaf is crisscross the setting each other.

Furthermore, a plurality of movable idler wheels are fixedly arranged on the peripheral corners below the base and are self-locking universal wheels, two groups of fixing parts with the same structure are symmetrically arranged on two sides of the base, and a push rod is arranged on one side of the base and fixedly connected with the base.

Furthermore, each group of fixing parts comprises a fixing slide rail, a fixing motor, a fixing plate and a screw joint rod, the fixing slide rail is installed on the base, the fixing motor is arranged on the fixing slide rail, the fixing motor is in sliding connection with the fixing slide rail, the screw joint rod is in screw joint with the base, the fixing plate is fixedly arranged at the lower end of the screw joint rod, and the upper end of the screw joint rod is fixedly connected with an output shaft of the fixing motor.

Further, the control box is electrically connected with the rotating motor, the lifting cylinder, the electric push rod, the heater, the stirring motor and the fixing motor.

Compared with the prior art, the invention has the beneficial effects that:

firstly, moving the equipment to a designated point, controlling a rotary adjusting component in an adjusting mechanism to adjust by a control box, moving a cryopreservation tube tray to a storage place for freezing cells by the rotary adjusting component, placing the cryopreservation tubes for storing the cells into the cryopreservation tube tray in sequence by a user, controlling a heater and a stirring component on a water bath box to work when the cryopreservation tube tray is completely filled, rapidly heating the water in the water bath box by the heater, mixing and stirring the water in the water bath box by the stirring component to ensure that the water is uniformly heated, and extending the adjusting component to work to stretch the cryopreservation tube tray into the water bath box when the water temperature is heated to a certain degree, the cell resuscitating device can resuscitate cells in a plurality of cryopreservation tubes at the same time in a semi-automatic mode, saves a large amount of time, has high efficiency and practicability, and can resuscitate the cells after the cells are rapidly heated to 37 ℃ to complete the cell resuscitating operation, and the separation water seepage plate is used for separating the stirring assembly from the cryopreservation tube bearing tray to prevent mistaken collision.

Secondly, the invention is used for adjusting the position of the freezing storage pipe bearing tray through the arrangement of the rotary adjusting component and the lifting adjusting component so as to conveniently take and place the freezing storage pipe, when the rotary adjusting is needed, the rotary motor in the installation box works to drive the rotary gear to rotate, the rotary gear drives the linkage gear meshed with the rotary gear to rotate, the linkage gear drives the rotary shaft to rotate, the rotary shaft rotates on the installation box and simultaneously drives the turntable to rotate, when the height adjusting is needed, the lifting cylinder in the support rod works to push the lifting rod to lift to complete the lifting operation, the arrangement of the extension adjusting component is used for extending the freezing storage pipe bearing tray into the water bath box to perform the resuscitation operation, at the moment, the electric push rod works to drive the U-shaped frame to push forwards, the U-shaped frame pushes the freezing storage pipe bearing tray fixedly connected with the U-shaped frame forwards into the water bath box, the guide seat and the guide rod play a role in assisting in guiding and pushing.

Thirdly, the plurality of placing grooves are formed in the cryopreservation pipe bearing tray and used for placing the plurality of cell cryopreservation pipes at the same time, so that the cells in the plurality of cell cryopreservation pipes can be recovered at the same time, and the limiting component is arranged and used for fixing and limiting the cell cryopreservation pipes, so that the cell cryopreservation pipes are prevented from shaking and influencing the recovery of the cells.

The stirring assembly is arranged to mix and stir water in the water bath tank to enable the water to be uniformly heated, when stirring operation is required, the stirring motor in the protective cover works to drive the stirring belt to rotate, the stirring belt drives one stirring shaft to synchronously rotate, the stirring shaft drives the stirring gear fixedly connected with one end of the stirring shaft to rotate, the stirring gear drives the other stirring gear meshed with the stirring shaft to relatively rotate, the other stirring gear drives the other stirring shaft to rotate, and the two stirring shafts relatively rotate to drive the stirring blades to relatively rotate to mix and stir the water in the water bath tank.

The device is convenient to move through the arrangement of the movable idler wheels below the base, the fixed part is arranged for limiting and fixing the device which moves to the designated position, accidental movement of the device is prevented, the push rod is arranged for more convenient movement adjustment, when the device needs to be fixed, the fixed motor on the fixed slide rail works to drive the threaded rod to rotate, the threaded rod rotates and adjusts on the base because the threaded rod is in threaded connection with the ground base, the threaded rod drives the fixed plate to move downwards, and when the device rotates to a certain degree, the fixed plate is abutted against the ground to complete the fixation of the device.

Drawings

FIG. 1 is a front view of the present invention;

FIG. 2 is a top view of the present invention;

FIG. 3 is a schematic perspective view of the present invention;

FIG. 4 is a top view of the adjustment mechanism of the present invention;

FIG. 5 is a cross-sectional view taken along line A-A of FIG. 4;

FIG. 6 is an enlarged view of FIG. 4 at B;

FIG. 7 is a top view of the water bath of the present invention;

fig. 8 is a cross-sectional view taken along line C-C of fig. 7.

Reference numerals:

the device comprises a base 1, a movable roller 11, a fixed part 12, a fixed slide rail 121, a fixed motor 122, a fixed plate 123, a screw rod 124, a push rod 13, an adjusting mechanism 2, a rotary adjusting component 21, an installation box 211, a rotary motor 212, a rotary gear 213, a rotary shaft 214, a linkage gear 215, a rotary disc 216, a lifting adjusting component 22, a support rod 221, a lifting rod 222, a lifting cylinder 223, a lifting plate 224, an extension adjusting component 23, an electric push rod 231, a guide seat 232, a guide rod 233, a U-shaped frame 234, a cryopreservation tube tray 3, a placing groove 31, a limiting part 32, a telescopic spring 321, a limiting block 322, a water bath box 4, a separation water seepage plate 41, a stirring component 42, a protective cover 421, a stirring motor 422, a stirring belt 423, a stirring shaft 424, a stirring gear 425, a stirring blade 426, a heater 43 and a control.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

Referring to fig. 1 to 8, an embodiment of the invention provides a cell resuscitator, which includes a base 1, an adjusting mechanism 2, a cryopreservation tube tray 3, a water bath tank 4 and a control box 5, wherein the adjusting mechanism 2, the water bath tank 4 and the control box 5 are all fixedly disposed on the base 1, the adjusting mechanism 2 can place the cryopreservation tube tray 3 into the water bath tank 4, the adjusting mechanism 2 includes a rotation adjusting assembly 21, a lifting adjusting assembly 22 and an extension adjusting assembly 23, the rotation adjusting assembly 21 is fixedly disposed on the base 1, the lifting adjusting assembly 22 is fixedly disposed on the rotation adjusting assembly 21, the extension adjusting assembly 23 is fixedly disposed on the lifting adjusting assembly 22, the cryopreservation tube tray 3 is fixedly disposed on the extension adjusting assembly 23, and a separation water seepage plate 41 is disposed on the water bath tank 4, The device comprises a stirring component 42 and a heater 43, wherein the separation water seepage plate 41 is fixedly arranged on the peripheral inner wall of the water bath box 4, the stirring component 42 is fixedly arranged on the water bath box 4 and is positioned below the separation water seepage plate 41, the heater 43 is fixedly arranged at the side of the water bath box 4 above the base 1, and the output end of the heater 43 extends to the inner bottom end of the water bath box 4. when frozen cells need to be revived, the device is firstly moved to a specified point, the control box 5 controls the rotary adjusting component 21 in the adjusting mechanism 2 to adjust, the rotary adjusting component 21 moves the cryopreservation tube bearing tray 3 to a storage position for cell freezing, the cryopreservation tubes for storing cells are sequentially placed into the cryopreservation tube bearing tray 3 by a user, after the cryopreservation tube bearing tray 3 is filled, the rotary adjusting component 21 works again, and the cryopreservation tube bearing tray 3 is adjusted to be above the water bath box 4, the height of the freezing pipe placing table is adjusted by the aid of the height adjusting assembly, people can conveniently take and place the freezing pipe placing table, at the moment, the heater 43 and the stirring assembly 42 on the control box 5 control the water bath box 4 to work, the heater 43 rapidly heats water in the water bath box 4, the stirring assembly 42 mixes and stirs the water in the water bath box 4 to enable the water to be heated uniformly, the adjusting assembly 23 is extended to work to stretch the freezing pipe bearing tray 3 into the water bath box 4 after the water is heated to a certain degree, cells are rapidly heated to 37 degrees and then revived, revived after the cells are completed, the water seepage plate 41 is separated to separate the stirring assembly 42 from the freezing pipe bearing tray 3, and mistaken collision is prevented.

The rotation adjusting assembly 21 comprises an installation box 211, a rotating motor 212, a rotating gear 213, a rotating shaft 214, a linkage gear 215 and a turntable 216, wherein the installation box 211 is hollow, the installation box 211 is fixedly arranged on the base 1, the rotating motor 212 is fixedly arranged on the inner bottom wall of the installation box 211, the rotating gear 213 is fixedly arranged on the output shaft of the rotating motor 212, the rotating shaft 214 is rotatably arranged on the installation box 211, the linkage gear 215 is fixedly arranged at the middle section position of the rotating shaft 214 in the installation box 211, the linkage gear 215 is meshed with the rotating gear 213, the turntable 216 is fixedly arranged at the outer top end of the rotating shaft 214, the position of the freezing tube bearing tray 3 is adjusted through the rotation adjusting assembly 21 so as to conveniently take and place the freezing tube, when rotation adjustment is needed, the rotating motor 212 in the installation box 211 works to drive the rotating gear 213 to rotate, the rotating gear 213 drives the linkage gear 215 engaged with the rotating gear to rotate, the linkage gear 215 drives the rotating shaft 214 to rotate, and the rotating shaft 214 rotates on the installation box 211 and drives the turntable 216 to rotate at the same time.

The elevation adjusting assembly 22 includes a support rod 221, an elevation rod 222, an elevation cylinder 223 and an elevation plate 224, the supporting rod 221 is a hollow structure, the supporting rod 221 is fixedly arranged above the turntable 216, the lifting cylinder 223 is fixedly disposed on the inner bottom wall of the supporting rod 221, the lifting rod 222 is disposed inside the supporting rod 221, the lifting rod 222 is in sliding fit with the support rod 221, the bottom of the lifting rod 222 is fixedly connected with the output end of the lifting cylinder 223, the lifting plate 224 is a strip-shaped plate, one end of the lifting plate 224 is fixedly arranged at the top of the lifting rod 222, the height adjusting assembly is arranged to adjust the height of the freezing storage tube placing table, so that people can take and place the freezing storage tube conveniently, when the height needs to be adjusted, the lifting cylinder 223 in the support rod 221 works to push the lifting rod 222 to lift, thereby completing the lifting operation.

The extension adjusting assembly 23 comprises an electric push rod 231, two guide seats 232, two guide rods 233 and a U-shaped frame 234, the electric push rod 231 is fixedly arranged at the other end of the lifting plate 224, the output end of the electric push rod 231 penetrates through the lifting plate 224, the two guide seats 232 and the two guide rods 233 are arranged, the two guide seats 232 are fixedly arranged on the lifting plate 224 and are respectively positioned at two sides of the electric push rod 231, the two guide rods 233 are respectively arranged on the two guide seats 232, the two guide rods 233 are respectively in sliding fit with the two guide seats 232, one ends of the two guide rods 233 penetrate through the lifting plate 224, the bottom of the U-shaped frame 234 is fixedly arranged at the output end of the electric push rod 231 and one end of the two guide rods 233, the extension adjusting assembly 23 is arranged to extend the cryopreservation tube tray 3 into the water bath tank 4 for resuscitation, at this time, the electric push rod 231 works to drive the U-shaped frame 234 to push forward, the U-shaped frame 234 pushes the cryopreservation pipe tray 3 fixedly connected with the U-shaped frame forward to the inside of the water bath tank 4, and the guide seat 232 and the guide rod 233 play roles in assisting the guiding and pushing operations.

The cryopreservation pipe bearing tray 3 is fixedly connected with two ends of the U-shaped frame 234, a plurality of placing grooves 31 are arranged in the cryopreservation pipe bearing tray 3, each placing groove 31 is internally provided with a same limiting part 32, each limiting part 32 comprises four expansion springs 321 and four limiting blocks 322, one end of each expansion spring 321 is fixedly arranged on the peripheral side wall in the placing groove 31, the four limiting blocks 322 are respectively fixedly arranged on the other ends of the four expansion springs 321, by arranging a plurality of placing grooves 31 in the cryopreservation tube tray 3 for placing a plurality of cell cryopreservation tubes at the same time, can be to the cell of simultaneously in a plurality of cell cryopreserved pipes recovering operation, spacing component 32's setting is used for carrying out fixed fibrous operation to the cell cryopreserved pipe, prevents rocking of cell cryopreserved pipe, influences recovering of cell.

The stirring assembly 42 comprises a protective cover 421, a stirring motor 422, a stirring belt 423, two stirring shafts 424, two stirring gears 425 and a plurality of stirring blades 426, the protective cover 421 covers one side of the water bath tank 4, the stirring motor 422 is fixedly arranged in the protective cover 421, the two stirring shafts 424 are arranged on the water bath tank 4 in a side-by-side rotating manner, two ends of the stirring shafts 424 penetrate through the side wall of the water bath tank 4 and extend out of the water bath tank 4, the two stirring gears 425 are respectively fixedly arranged at one end of the two stirring shafts 424, the stirring belt 423 is sleeved on an output shaft of the stirring motor 422 and one end of one of the stirring shafts 424, the stirring blades 426 are respectively arranged on the two stirring shafts 424 at intervals, the stirring blades 426 on the stirring shafts 424 are arranged in a mutually staggered manner, the stirring assembly 42 is arranged to mix and stir water in the water bath tank 4 to enable the water to be heated uniformly, when the stirring operation is needed, the stirring motor 422 in the protective cover 421 works to drive the stirring belt 423 to rotate, the stirring belt 423 drives one of the stirring shafts 424 to synchronously rotate, the stirring shaft 424 drives the stirring gear 425 fixedly connected with one end of the stirring shaft 424 to rotate, the stirring gear 425 drives the other stirring gear 425 engaged with the stirring gear to relatively rotate, the other stirring gear 425 drives the other stirring shaft 424 to rotate, and the relative rotation of the two stirring shafts 424 drives the plurality of stirring blades 426 to relatively rotate to mix and stir the water in the water bath tank 4.

The fixed a plurality of removal gyro wheel 11 that is provided with, a plurality of on the corner all around of base 1 below remove gyro wheel 11 and be self-locking type universal wheel, bilateral symmetry on the base 1 is provided with two sets of fixed part 12 that the structure is the same, one side of base 1 is provided with push rod 13, push rod 13 with base 1 fixed connection, the setting of removing gyro wheel 11 are used for conveniently removing the operation with equipment, and fixed part 12's setting is used for carrying out spacing fixed with the equipment that moves the assigned position, prevents that equipment from taking place unexpected removal, and push rod 13's setting is used for more convenient removal regulation.

Each set of the fixing parts 12 comprises a fixed slide rail 121, a fixed motor 122, a fixed plate 123 and a screw rod 124, the fixed slide rail 121 is installed on the base 1, the fixed motor 122 is arranged on the fixed slide rail 121, the fixed motor 122 is slidably connected with the fixed slide rail 121, the screw rod 124 is screwed on the base 1, the fixing plate 123 is fixedly disposed at the lower end of the screw rod 124, the upper end of the screw rod 124 is fixedly connected with the output shaft of the fixed motor 122, when the fixing is needed, the fixing motor 122 on the fixing rail 121 works to drive the screw rod 124 to rotate, because the screw rod 124 is screwed with the ground, therefore, the screw rod 124 is adjusted to rotate on the base 1, the screw rod 124 drives the fixing plate 123 to move downwards, and when the screw rod rotates to a certain degree, the fixing plate 123 abuts against the ground to complete the fixing of the equipment.

The control box 5 is electrically connected with the rotating motor 212, the lifting cylinder 223, the electric push rod 231, the heater 43, the stirring motor 422 and the fixed motor 122, and the control box 5 is electrically connected with the rotating motor 212, the lifting cylinder 223, the electric push rod 231, the heater 43, the stirring motor 422 and the fixed motor 122 to control the operation.

The working principle of the invention is as follows: when the invention needs to recover frozen cells, firstly, the device is moved to a designated point, the control box 5 controls the rotary adjusting component 21 in the adjusting mechanism 2 to adjust, the rotary adjusting component 21 moves the cryopreservation tube tray 3 to a storage place for freezing the cells, the cryopreservation tubes for storing the cells are sequentially placed into the cryopreservation tube tray 3 by a user, after the cryopreservation tube tray 3 is completely filled, the rotary adjusting component 21 works again, the cryopreservation tube tray 3 is adjusted to be above the water bath box 4, the height adjusting component is arranged to adjust the height of the placing table of the cryopreservation tubes, so that people can conveniently take and place the cryopreservation tubes, at the moment, the control box 5 controls the heater 43 and the stirring component 42 on the water bath box 4 to work, the heater 43 rapidly heats the water in the water bath box 4, the stirring component 42 mixes and stirs the water in the water bath box 4 to uniformly heat the water, when the water temperature is heated to a certain degree, the extension adjusting component 23 works to extend the cryopreservation tube tray 3 into the water bath box 4, so that the cells are rapidly heated to 37 ℃ and then recovered, the recovery operation of the cells is completed, the separation water seepage plate 41 is used for separating the stirring component 42 from the cryopreservation tube tray 3, and the mistaken collision is prevented.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, those skilled in the art will appreciate that; the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A cell resuscitator, comprising: the device is characterized by comprising a base (1), an adjusting mechanism (2), a cryopreservation pipe bearing tray (3), a water bath box (4) and a control box (5), wherein the adjusting mechanism (2), the water bath box (4) and the control box (5) are fixedly arranged on the base (1), the adjusting mechanism (2) can place the cryopreservation pipe bearing tray (3) into the water bath box (4), the adjusting mechanism (2) comprises a rotary adjusting component (21), a lifting adjusting component (22) and an extension adjusting component (23), the rotary adjusting component (21) is fixedly arranged on the base (1), the lifting adjusting component (22) is fixedly arranged on the rotary adjusting component (21), the extension adjusting component (23) is fixedly arranged on the lifting adjusting component (22), the cryopreservation pipe bearing tray (3) is fixedly arranged on the extension adjusting component (23), be equipped with on water bath (4) and separate infiltration board (41), stirring subassembly (42) and heater (43), separate infiltration board (41) and fix the inner wall that sets up all around at water bath (4), stirring subassembly (42) is fixed to be set up on water bath (4) and is located separate the below of infiltration board (41), heater (43) is fixed to be set up the side of base (1) top water bath (4), the output of heater (43) extends to the inside bottom of water bath (4).

2. A cell resuscitator, according to claim 1, wherein: the rotation adjusting component (21) comprises a mounting box (211), a rotating motor (212), a rotating gear (213), a rotating shaft (214), a linkage gear (215) and a turntable (216), the interior of the installation box (211) is of a hollow structure, the installation box (211) is fixedly arranged on the base (1), the rotating motor (212) is fixedly arranged on the inner bottom wall of the mounting box (211), the rotating gear (213) is fixedly arranged on an output shaft of the rotating motor (212), the rotating shaft (214) is rotatably installed on the installation box (211), the linkage gear (215) is fixedly arranged at the middle section position of the rotating shaft (214) in the installation box (211), the linkage gear (215) is meshed with the rotating gear (213), and the rotary disc (216) is fixedly arranged at the top end of the outer part of the rotating shaft (214).

3. A cell resuscitator according to claim 2, wherein: lifting adjusting subassembly (22) includes bracing piece (221), lifter (222), lift cylinder (223) and lifter plate (224), bracing piece (221) is hollow structure, bracing piece (221) is fixed to be set up carousel (216) top, lift cylinder (223) are fixed to be set up on the interior diapire of bracing piece (221), lifter (222) are located inside bracing piece (221), lifter (222) with sliding fit between bracing piece (221), the bottom of lifter (222) with the output fixed connection of lift cylinder (223), lifter plate (224) are rectangular shape panel, the one end of lifter plate (224) is fixed to be set up lifter (222) top.

4. A cell resuscitator according to claim 3, wherein: the extension adjusting component (23) comprises an electric push rod (231), a guide seat (232), a guide rod (233) and a U-shaped frame (234), the electric push rod (231) is fixedly arranged at the other end of the lifting plate (224), the output end of the electric push rod (231) penetrates through the lifting plate (224), two guide seats (232) and two guide rods (233) are arranged, the two guide seats (232) are fixedly arranged on the lifting plate (224) and are respectively positioned at two sides of the electric push rod (231), the two guide rods (233) are respectively arranged on the two guide seats (232), the two guide rods (233) are respectively in sliding fit with the two guide seats (232), one ends of the two guide rods (233) penetrate through the lifting plate (224), the bottom of the U-shaped frame (234) is fixedly provided with the output end of the electric push rod (231) and one end of the two guide rods (233).

5. A cell resuscitator according to claim 4, wherein: cryopreserved pipe holds tray (3) with both ends fixed connection on U type frame (234), be provided with a plurality of standing groove (31) in cryopreserved pipe holds tray (3), every all be provided with a same spacing part (32) in standing groove (31), every spacing part (32) include four expanding spring (321) and four stopper (322), four the one end of expanding spring (321) is fixed to be set up on the lateral wall all around in standing groove (31), four stopper (322) are fixed respectively to be set up four on the other end of expanding spring (321).

6. A cell resuscitator, according to claim 1, wherein: stirring subassembly (42) includes protection casing (421), agitator motor (422), stirring belt (423), two (mixing) shafts (424), two stirring gear (425) and a plurality of stirring leaf (426), protection casing (421) cover is established one side on water bath (4), agitator motor (422) are fixed to be set up in protection casing (421), two (mixing) shaft (424) rotate side by side and set up on water bath (4), two the both ends of (mixing) shaft (424) all run through water bath (4) lateral wall and extend to outside water bath (4), two stirring gear (425) are fixed respectively to be set up on one of two (mixing) shafts (424), stirring belt (423) cover is established on one of the output shaft of agitator motor (422) and one of them (mixing) shaft (424), a plurality of stirring leaf (426) interval respectively sets up on two (mixing) shafts (424), the stirring blades (426) on the two stirring shafts (424) are arranged in a staggered mode.

7. A cell resuscitator according to claim 6, wherein: the movable trolley is characterized in that a plurality of movable idler wheels (11) and a plurality of movable idler wheels (11) are fixedly arranged on the corners around the lower portion of the base (1), the movable idler wheels (11) are self-locking universal wheels, two groups of fixing parts (12) with the same structure are symmetrically arranged on two sides of the base (1), a push rod (13) is arranged on one side of the base (1), and the push rod (13) is fixedly connected with the base (1).

8. A cell resuscitator, according to claim 7, wherein: each group of the fixing part (12) comprises a fixing slide rail (121), a fixing motor (122), a fixing plate (123) and a bolt joint rod (124), the fixing slide rail (121) is installed on the base (1), the fixing motor (122) is arranged on the fixing slide rail (121), the fixing motor (122) is connected with the fixing slide rail (121) in a sliding mode, the bolt joint rod (124) is arranged on the base (1) in a threaded mode, the fixing plate (123) is fixedly arranged at the lower end of the bolt joint rod (124), and the upper end of the bolt joint rod (124) is fixedly connected with an output shaft of the fixing motor (122).