CN110591702B - Preparation method and application of aggregation-induced luminescent silver nanocluster - Google Patents

Preparation method and application of aggregation-induced luminescent silver nanocluster Download PDF

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CN110591702B
CN110591702B CN201910888226.2A CN201910888226A CN110591702B CN 110591702 B CN110591702 B CN 110591702B CN 201910888226 A CN201910888226 A CN 201910888226A CN 110591702 B CN110591702 B CN 110591702B
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张彦
吕玫
高鹏飞
李天栋
双少敏
张国梅
董川
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Abstract

The invention discloses a preparation method and application of aggregation-induced luminescent silver nanoclusters, wherein the preparation method comprises the following steps: mixing 5-17.5 parts by volume of 20mmol/L N-acetyl-L-cysteine aqueous solution and 20 parts by volume of 2.5mmol/L silver nitrate solution, uniformly stirring the mixed solution, controlling the heating temperature to be 60-80 ℃, refluxing for 12-48h, cooling, taking out, and freeze-drying to obtain the aggregation-induced luminescent silver nanoclusters, wherein the fluorescence quantum yield can be as high as 0.44. The preparation method is simple in preparation process, simple in reaction condition and environment-friendly, and the prepared aggregation-induced luminescent silver nanocluster has good aggregation-induced luminescent property, high fluorescence quantum yield, microsecond-level long service life and high content of Ag (I). The aggregation-induced luminescent silver nanocluster prepared by the invention can be used for detecting tetracycline.

Description

Preparation method and application of aggregation-induced luminescent silver nanocluster
Technical Field
The invention relates to preparation of silver nanoclusters, in particular to a preparation method and application of aggregation-induced luminescent silver nanoclusters.
Background
Tetracycline (TC) is a broad spectrum antibacterial drug found in the middle of the 20 th century. Because tetracycline has the advantages of various antibacterial properties, low cost, good oral absorption and the like, the tetracycline is widely applied to the industries such as the medical industry, the breeding industry, the meat processing industry and the like. However, as a result of investigation, when tetracycline circulates in the environment, on one hand, ecological pollution is caused, and on the other hand, after the tetracycline is contacted with microorganisms or bacteria in the environment, the microorganisms or bacteria generate resistance genes, so that the inhibition capability of the tetracycline to the bacteria is reduced. More seriously, the microorganisms producing antibiotic resistance can directly or indirectly enter the human body through the ecological cycle, resulting in reduced antibiotic action and threat to human health. Common tetracycline detection methods include Capillary Electrophoresis (CE), High Performance Liquid Chromatography (HPLC), spectrophotometry, and Chemiluminescence (CL). However, these methods have the disadvantages of being time-consuming, expensive in apparatus and cumbersome to operate. In recent years, fluorescence analysis methods have been widely studied in the field of analytical detection due to the advantages of easy operation, high sensitivity, fast signal response, low cost, real-time detection, almost no damage to samples, and the like. Therefore, it is very important to construct a high-selectivity and high-accuracy fluorescence analysis method for detecting tetracycline.
Organic fluorophores with conjugated structures cause quenching effects in the aggregated state, whereas luminescent materials with aggregation-induced emission may emit strong light in the aggregated state or in the solid state. In recent years, the unique properties of such luminescent materials have made them overcome the drawbacks of the aggregation quenching effect of conventional luminescent materials, and such aggregation-induced luminescent materials have attracted attention from a wide range of researchers. Currently, most of the research on aggregation-induced emission phenomenon focuses on organic molecules such as tetraphenylethylene and hexaphenylsilole. In recent years, there have been reports of nanocluster aggregation-induced emission materials, and Jiezhou et al adopted Au3+The prepared silver nanoclusters are induced to obtain aggregation type silver nanoclusters (Talanta,2018,188, 623-629) with strong luminescence, and after the nanoclusters are aggregated, intramolecular vibration and rotation of the ligand can be inhibited, so that the non-radiative relaxation behavior of an excited state related to the ligand is inhibited, and the luminescence performance of the silver nanoclusters is obviously enhanced. However, an aggregate having long-life properties is obtainedInducing luminescent materials is very challenging. In a recent study (Journal of Materials Chemistry B,2018,6, 3927-3933), Saifei Pan et al prepared hydrophobic silver nanoclusters protected by thiosalicylic acid under the protection of nitrogen and self-assembled at 0 ℃ to obtain aggregation-induced emission nanomaterials, the quantum yield was 0.25 and the fluorescence lifetime was 7.65 μ s. Although the aggregation-induced emission material with long service life is obtained, the whole process not only needs the protection of nitrogen, but also needs self-assembly at 0 ℃, and the process is complicated.
Disclosure of Invention
In view of this, the present invention aims to provide a preparation method and an application of aggregation-induced luminescent silver nanoclusters, which aim to overcome the defects of complicated preparation process, low fluorescence quantum yield and short fluorescence lifetime existing in the existing preparation process of silver nanoclusters.
In order to achieve the purpose of the invention, the following technical scheme is adopted:
a preparation method of aggregation-induced luminescent silver nanoclusters comprises the following steps: mixing 5-17.5 parts by volume of 20mmol/L N-acetyl-L-cysteine aqueous solution and 20 parts by volume of 2.5mmol/L silver nitrate solution, uniformly stirring the mixed solution, controlling the heating temperature to be 60-80 ℃, refluxing for 12-48h, cooling, taking out, and freeze-drying to obtain the aggregation-induced luminescent silver nanoclusters.
Preferably, the method comprises the following steps: mixing 5-12.5 parts by volume of 20mmol/L N-acetyl-L-cysteine aqueous solution and 20 parts by volume of 2.5mmol/L silver nitrate solution, uniformly stirring the mixed solution, controlling the heating temperature to be 70-80 ℃, refluxing for 12-36h, cooling, taking out, and freeze-drying to obtain the aggregation-induced luminescent silver nanoclusters.
More preferably, the method comprises the following steps: mixing 5 parts by volume of 20mmol/L N-acetyl-L-cysteine aqueous solution and 20 parts by volume of 2.5mmol/L silver nitrate solution, uniformly stirring the mixed solution, controlling the heating temperature to be 80 ℃ and refluxing for 24 hours, cooling, taking out, and freeze-drying to obtain the aggregation-induced luminescent silver nanocluster.
The aggregation-induced luminescent silver nanocluster prepared by the invention is applied to detection of tetracycline.
The invention has the beneficial effects that:
(1) the invention adopts N-acetyl-L-cysteine as a reducing agent and a ligand protective agent to prepare the aggregation-induced luminescent silver nanocluster with good luminescence property, the preparation process is simple and environment-friendly, the addition of chemical reagents such as common reducing agents of sodium borohydride, ascorbic acid, a surfactant and the like is avoided, the prepared aggregation-induced luminescent silver nanocluster has the characteristics of aggregation-induced luminescence and can generate strong fluorescence, the fluorescence emission peak of the aggregation-induced luminescent silver nanocluster is about 490nm, when the aggregation-induced luminescent silver nanocluster is observed by a black background under ultraviolet light, the strong blue-green fluorescence is presented, and the fluorescence quantum yield can be as high as 0.44.
(2) The aggregation-induced luminescent silver nanocluster prepared by the invention has the advantages of average particle size of 1.36nm, small size, uniform particle size distribution and good photobleaching resistance.
(3) The aggregation-induced luminescent silver nanocluster prepared by the invention has high sensitivity and selectivity on tetracycline, can be applied to tetracycline detection, and has the detection limit of 0.47 mu M.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the invention and, together with the description, serve to explain the invention and not to limit the invention. In the drawings:
fig. 1 is a process of forming aggregation-induced luminescent silver nanoclusters;
fig. 2 is a transmission electron microscope photograph of aggregation-induced luminescent silver nanoclusters of example 8;
fig. 3 is an ultraviolet absorption spectrum and fluorescence excitation and emission spectrum of aggregation-induced luminescent silver nanoclusters of example 8;
fig. 4 is a graph of the photostability of aggregation-induced luminescent silver nanoclusters of example 8;
FIG. 5 is an X-ray photoelectron spectrum of aggregation-induced luminescent silver nanoclusters of example 8;
fig. 6 is a transmission electron microscope photograph of comparative example silver nanoclusters.
Fig. 7(a) is a working curve of the aggregation-induced luminescent silver nanoclusters of example 8 in response to tetracycline;
FIG. 7(b) is a linear relationship between the change value of the fluorescence intensity in logarithm of the aggregation-induced luminescent silver nanocluster of example 8 and the tetracycline concentration;
FIG. 8 is a bar graph of fluorescence of aggregation-induced luminescent silver nanoclusters of example 8 after interaction with various small molecules;
fig. 9 is a bar graph of fluorescence of aggregation-induced luminescent silver nanoclusters of example 8 after interaction with various metal ions.
Detailed Description
It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present invention will be described in detail below with reference to the embodiments with reference to the attached drawings.
In order to make the technical solutions better understood by those skilled in the art, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only partial embodiments of the present application, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
As shown in fig. 1, the formation process of the aggregation-induced luminescent silver nanoclusters of the present invention is: firstly, N-acetyl-L-cysteine (NAC) is coordinated with monovalent silver Ag (I) through sulfydryl (-SH) under stirring at room temperature to form Ag (I) -thiolate complex, or Ag (I) is coordinated with other groups-X (-COOH/NO) in solution2 -) Coordination occurs to form a coordination compound; then under the heating reflux condition of 60-80 ℃, N-acetyl-L-cysteine selectively reduces Ag (I) into Ag (0) due to the high affinity between Ag (I) and zero-valent silver Ag (0), and the Ag (0) is separated from Ag (I) -thiolate complex by NAC to form Ag (0) -Ag (I) -thiolate intermediate; ag (0) -Ag (I) -S when the reflux is carried out under the condition of continuously controlling the heating temperature to be 60-80 DEG CThe alkoxide intermediates are slowly aggregated by Ag (0) collision and fusion to form Ag (0) core and shell structure of Ag (i) -thiolate complex, forming aggregation-induced emission type silver nanoclusters as shown in fig. 2. The luminescence behavior of the silver nanoclusters is caused by charge transfer from a ligand to silver and from the ligand to monovalent silver and then to zero-valent silver, after the silver nanoclusters are aggregated, the vibration and rotation in ligand N-acetyl-L-cysteine molecules are inhibited, so that the excited state non-radiative relaxation behavior related to the ligand is inhibited, the luminescence performance of the silver nanoclusters is obviously enhanced, and after the silver nanoclusters are aggregated, the charge transfer can be carried out among different clusters, so that the fluorescence performance of the silver nanoclusters is further enhanced.
Example 1
A preparation method of aggregation-induced luminescent silver nanoclusters comprises the following steps: mixing 5mL of 20mmol/L N-acetyl-L-cysteine aqueous solution and 20mL of 2.5mmol/L silver nitrate aqueous solution, uniformly stirring the mixed solution, controlling the heating temperature to be 60 ℃ and refluxing for 24h, cooling, taking out, and freeze-drying to obtain the aggregation-induced luminescent silver nanocluster. The fluorescence emission peak of the aggregation-induced luminescent silver nanocluster is about 516nm, and when the aggregation-induced luminescent silver nanocluster is observed on a black background under ultraviolet light, relatively strong green fluorescence is presented, the quantum yield is 0.35, and the fluorescence lifetime is 9.15 mu s.
Example 2
A preparation method of aggregation-induced luminescent silver nanoclusters comprises the following steps: mixing 5mL of 20mmol/L N-acetyl-L-cysteine aqueous solution and 20mL of 2.5mmol/L silver nitrate aqueous solution, uniformly stirring the mixed solution, controlling the heating temperature to be 70 ℃ and refluxing for 24h, cooling, taking out, and freeze-drying to obtain the aggregation-induced luminescent silver nanocluster. The fluorescence emission peak of the aggregation-induced luminescent silver nanocluster is about 494nm, and the aggregation-induced luminescent silver nanocluster presents strong blue-green fluorescence when observed with a black background under ultraviolet light, wherein the quantum yield is 0.40, and the fluorescence lifetime is 10.55 mu s.
Example 3
A preparation method of aggregation-induced luminescent silver nanoclusters comprises the following steps: mixing 17.5mL of 20mmol/L N-acetyl-L-cysteine aqueous solution and 20mL of 2.5mmol/L silver nitrate aqueous solution, uniformly stirring the mixed solution, controlling the heating temperature to be 80 ℃ and refluxing for 48h, cooling, taking out, and freeze-drying to obtain the aggregation-induced luminescent silver nanocluster. The fluorescence emission peak of the aggregation-induced luminescent silver nanocluster is about 492nm, and when the aggregation-induced luminescent silver nanocluster is observed on a black background under ultraviolet light, intense blue-green fluorescence is presented, the quantum yield is 0.39, and the fluorescence lifetime is 9.22 mu s.
Example 4
A preparation method of aggregation-induced luminescent silver nanoclusters comprises the following steps: mixing 15mL of 20mmol/L N-acetyl-L-cysteine aqueous solution and 20mL of 2.5mmol/L silver nitrate aqueous solution, uniformly stirring the mixed solution, controlling the heating temperature to be 80 ℃ and refluxing for 36h, cooling, taking out, and freeze-drying to obtain the aggregation-induced luminescent silver nanocluster. The fluorescence emission peak of the aggregation-induced luminescent silver nanocluster is about 492nm, and when the aggregation-induced luminescent silver nanocluster is observed on a black background under ultraviolet light, intense blue-green fluorescence is presented, the quantum yield is 0.40, and the fluorescence lifetime is 9.85 microseconds.
Example 5
A preparation method of aggregation-induced luminescent silver nanoclusters comprises the following steps: mixing 12.5mL of 20mmol/L N-acetyl-L-cysteine aqueous solution and 20mL of 2.5mmol/L silver nitrate aqueous solution, uniformly stirring the mixed solution, controlling the heating temperature to be 80 ℃ and refluxing for 24h, cooling, taking out, and freeze-drying to obtain the aggregation-induced luminescent silver nanocluster. The fluorescence emission peak of the aggregation-induced luminescent silver nanocluster is about 492nm, and when the aggregation-induced luminescent silver nanocluster is observed on a black background under ultraviolet light, intense blue-green fluorescence is presented, the quantum yield is 0.41, and the fluorescence lifetime is 10.20 mu s.
Example 6
A preparation method of aggregation-induced luminescent silver nanoclusters comprises the following steps: mixing 10mL of 20mmol/L N-acetyl-L-cysteine aqueous solution and 20mL of 2.5mmol/L silver nitrate aqueous solution, uniformly stirring the mixed solution, controlling the heating temperature to be 80 ℃ and refluxing for 24h, cooling, taking out, and freeze-drying to obtain the aggregation-induced luminescent silver nanocluster. The fluorescence emission peak of the aggregation-induced luminescent silver nanocluster is about 490nm, and when the aggregation-induced luminescent silver nanocluster is observed under ultraviolet light on a black background, intense blue-green fluorescence is presented, the quantum yield is 0.42, and the fluorescence lifetime is 10.55 mu s.
Example 7
A preparation method of aggregation-induced luminescent silver nanoclusters comprises the following steps: mixing 7.5mL of 20mmol/L N-acetyl-L-cysteine aqueous solution and 20mL of 2.5mmol/L silver nitrate aqueous solution, uniformly stirring the mixed solution, controlling the heating temperature to be 80 ℃ and refluxing for 12h, cooling, taking out, and freeze-drying to obtain the aggregation-induced luminescent silver nanocluster. The fluorescence emission peak of the aggregation-induced luminescent silver nanocluster is about 490nm, and when the aggregation-induced luminescent silver nanocluster is observed under ultraviolet light and a black background, intense blue-green fluorescence is presented, the quantum yield is 0.42, and the fluorescence lifetime is 10.65 microseconds.
Example 8
A preparation method of aggregation-induced luminescent silver nanoclusters comprises the following steps: mixing 5mL of 20mmol/L N-acetyl-L-cysteine aqueous solution and 20mL of 2.5mmol/L silver nitrate aqueous solution, uniformly stirring the mixed solution, controlling the heating temperature to be 80 ℃ and refluxing for 24h, cooling, taking out, and freeze-drying to obtain the aggregation-induced luminescent silver nanocluster.
The size and shape of the synthesized aggregation-induced luminescent silver nanoclusters (AgNCs) were characterized by a Transmission Electron Microscope (TEM), and as shown in fig. 2, the synthesized aggregation-induced luminescent silver nanoclusters exhibited an aggregated state, and their luminescent aggregates consisted of smaller silver nanoclusters with an average particle size of 1.36nm, indicating that the strong luminescent properties of the synthesized aggregation-induced luminescent silver nanoclusters were caused by aggregation induction.
500. mu.L of BR buffer solution (50mmol/L, pH 5), 500. mu.L of ethanol mixed solution system and 100. mu.L of aggregation-induced luminescent silver nanocluster solution were added to a fluorescence cuvette, and its ultraviolet absorption spectrum and fluorescence excitation and emission spectrum were measured, as shown in FIG. 3, with the fluorescence emission peak of the aggregation-induced luminescent silver nanocluster being 494 nm.
The aggregation-induced luminescent silver nanocluster solution is subjected to a photobleaching resistance experiment, as shown in fig. 4, the aggregation-induced luminescent silver nanocluster solution can maintain good luminescent performance within 30min, which indicates that the photobleaching resistance is good.
The aggregation-induced luminescent silver nanocluster shows strong blue-green fluorescence when observed with a black background under ultraviolet light, the quantum yield is 0.44, and the fluorescence lifetime is 11.08 microseconds.
The chemical valence state of the synthesized aggregation-induced luminescent silver nanoclusters was characterized by X-ray photoelectron spectroscopy (XPS), and fig. 5 is an XPS spectrogram of Ag3d, showing Ag3d5/2And Ag3d3/2The maximum peak values of the binding energy of (a) are 367.68eV and 373.68eV, respectively. 367.68eV is between 367.5eV (Ag (I)) and 368.2eV (Ag (0)), the synthesized aggregation-induced luminescent silver nanoclusters are deduced to be composed of Ag (I) and Ag (0), and the ratio of Ag (I) to Ag (0) of the synthesized aggregation-induced luminescent silver nanoclusters can be deduced to be 2.25:1 by further peak separation of XPS as shown in FIG. 5.
Comparative example:
a method for preparing silver nanoclusters, comprising the steps of:
mixing 20mL of 1mmol/L silver nitrate solution and 80mL of 0.125mmol/L N-acetyl-L-cysteine aqueous solution to form a mixed solution, and then stirring the mixed solution vigorously to obtain 8.8mg of NaBH4Adding into the mixed solution to obtain yellow silver colloid solution. Stirring the obtained yellow silver colloid solution for 2 hours at room temperature in the dark to ensure that the N-acetyl-L-cysteine is self-assembled on the surface of the silver cluster, and then storing the yellow silver colloid solution at 4 ℃ and keeping out of the sun overnight to obtain the silver nanocluster. The silver nanoclusters were prepared as a 0.1mg/mL aqueous solution of silver nanoclusters, and the aqueous solution was dropped on a copper mesh with a carbon film, vacuum-dried, and then subjected to TEM testing, as shown in fig. 6, where the silver nanoclusters had a particle diameter of about 12nm and exhibited no fluorescence when observed on a black background under ultraviolet light.
This comparative example is a comparative example to example 8, which is further illustrated by the use of NaBH in order to further illustrate the advantages of the process of the present invention4AgNO as reducing agent3The monovalent silver Ag (I) is reduced to zero-valent silver Ag (0), and N-acetyl-L-cysteine is used as a ligand to synthesize the silver nanocluster. By comparison, with NaBH4The preparation method of the reducing agent can not obtain the fluorescent nano material.
Example 9
Use of aggregation-induced luminescent silver nanoclusters prepared as in example 8 in the detection of tetracycline.
The aggregation-induced luminescent silver nanoclusters prepared in example 8 were configured into 0.1mg/mL aggregation-induced luminescent silver nanocluster solution, 500 μ L of ethanol, 500 μ L of BR buffer solution (50mmol/L, pH 5), and 100 μ L of aggregation-induced luminescent silver nanocluster solution were injected into a fluorescence cuvette, stirred until uniform mixing was achieved, and after no bubble was generated, the tetracycline solution was added to the fluorescence cuvette in the order of concentration from small to large, and the fluorescence spectra thereof were measured with 386nm as the excitation wavelength, respectively. As shown in fig. 7(a), as the concentration of tetracycline increases, the fluorescence of the silver nanoclusters is gradually quenched; as shown in FIG. 7(b), the logarithmic change in fluorescence intensity was plotted as Log (F) against the concentration of tetracycline0is/F) wherein F0And F respectively represent the fluorescence intensity of the silver nanoclusters in the absence and presence of tetracycline, and the detection limit of tetracycline is 0.47 μ M (calculated according to the formula LOD ═ 3 σ/k, σ is standard deviation of the fluorescence intensity values of the silver nanoclusters for 11 times, and the k value is the slope of the fitted straight line in fig. 7 (b)). The regression equation of the silver nanoclusters obtained by linear fitting is: y is 0.0139+0.004X, and the linear coefficient is R20.997. Based on the method, the aggregation-induced luminescent silver nanocluster can be applied to detection of tetracycline in milk.
Example 10
The aggregation-inducible luminescent silver nanoclusters prepared in example 8 were configured into an aggregation-inducible luminescent silver nanocluster solution of 0.1mg/mL, 500 μ L of ethanol, 500 μ L of BR buffer solution (50mmol/L, pH 5), and 100 μ L of the aggregation-inducible luminescent silver nanocluster solution were injected into a fluorescence cuvette, and first, tetracycline was added thereto to measure the fluorescence intensity thereof as a blank; secondly, respectively adding 20 common anions and cations and 15 common micromolecules (the concentration of the coexisting ions is 20 times that of the tetracycline) and other potential interference substances, measuring and recording the fluorescence intensity value; tetracycline was added to the solution, and the fluorescence intensity was measured and recorded. Measuring fluorescence spectra with 386nm as excitation wavelength, respectively, and drawing a bar graph of fluorescence intensity at 494nm corresponding to different small molecules, as shown in FIG. 8; the different ions were plotted against the intensity of the fluorescence at 494nm as shown in FIG. 9. Experiments prove that other small molecules and ions do not interfere the detection of the system on the tetracycline.
The 15 small molecules are DL-cysteine (DL-Cys), DL-histidine (DL-His), L-glutamine (L-Glu), tyrosine (Tyr), phenylalanine (Phe), Glutathione (GSH), vitamin C, vitamin B1, vitamin B2, vitamin B6, glucose (Glc), sorbic acid (Sorbicic), citric acid (Citricacid), DL-mandelic acid (DL-Mandelicacid) and erythromycin (E), respectively. 20 kinds of anions and cations are respectively PO4 3-、CO3 2-、HCO3 -、F-、SO4 2-、Cl-、NO2 -、Ac-、SCN-、C2O4 2-、Ca2+、Mg2+、K+、Zn2+、Mn2+、Na+、Sn2+、Al3+、Fe3+And Cu2+。
The preparation method is simple in preparation process, simple in reaction condition and environment-friendly, and the prepared aggregation-induced luminescent silver nanocluster has good aggregation-induced luminescent property, high fluorescence quantum yield, microsecond-level long service life and high content of Ag (I). The aggregation-induced luminescent silver nanocluster prepared by the invention can be used for detecting tetracycline.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement or combination made within the spirit and principle of the present invention shall fall within the protection scope of the present invention.

Claims (3)

1. A preparation method of aggregation-induced luminescent silver nanoclusters is characterized by comprising the following steps: mixing 5-17.5 parts by volume of 20mmol/L N-acetyl-L-cysteine aqueous solution and 20 parts by volume of 2.5mmol/L silver nitrate solution, uniformly stirring the mixed solution, controlling the heating temperature to be 60-80 ℃, refluxing for 12-48h, cooling, taking out, and freeze-drying to obtain the aggregation-induced luminescent silver nanoclusters.
2. The method for preparing aggregation-induced luminescent silver nanoclusters according to claim 1, comprising the steps of: mixing 5-12.5 parts by volume of 20mmol/L N-acetyl-L-cysteine aqueous solution and 20 parts by volume of 2.5mmol/L silver nitrate solution, uniformly stirring the mixed solution, controlling the heating temperature to be 70-80 ℃, refluxing for 12-36h, cooling, taking out, and freeze-drying to obtain the aggregation-induced luminescent silver nanoclusters.
3. The method for preparing aggregation-induced luminescent silver nanoclusters according to claim 1 or 2, comprising the steps of: mixing 5 parts by volume of 20mmol/L N-acetyl-L-cysteine aqueous solution and 20 parts by volume of 2.5mmol/L silver nitrate solution, uniformly stirring the mixed solution, controlling the heating temperature to be 80 ℃ and refluxing for 24 hours, cooling, taking out, and freeze-drying to obtain the aggregation-induced luminescent silver nanocluster.
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